TY  - CONF
AU  - Nešić, M. D.
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Popović, I.
AU  - Soto, J.
AU  - Algarra, M.
AU  - Ralić, V.
AU  - Abu el Rub, Anamarija
AU  - Matijević, M.
AU  - Stepić, Milutin
AU  - Petković, M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11809
AB  - Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
T1  - Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11809
ER  - 

@conference{
author = "Nešić, M. D. and Filipović Tričković, Jelena and Valenta Šobot, Ana and Žakula, Jelena and Korićanac, Lela and Popović, I. and Soto, J. and Algarra, M. and Ralić, V. and Abu el Rub, Anamarija and Matijević, M. and Stepić, Milutin and Petković, M.",
year = "2023",
abstract = "Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade",
title = "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11809"
}

Nešić, M. D., Filipović Tričković, J., Valenta Šobot, A., Žakula, J., Korićanac, L., Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, A., Matijević, M., Stepić, M.,& Petković, M.. (2023). Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
Belgrade : Vinča Institute of Nuclear Sciences., 79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809

Nešić MD, Filipović Tričković J, Valenta Šobot A, Žakula J, Korićanac L, Popović I, Soto J, Algarra M, Ralić V, Abu el Rub A, Matijević M, Stepić M, Petković M. Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade. 2023;:79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

Nešić, M. D., Filipović Tričković, Jelena, Valenta Šobot, Ana, Žakula, Jelena, Korićanac, Lela, Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, Anamarija, Matijević, M., Stepić, Milutin, Petković, M., "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles" in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade (2023):79-79,
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

TY  - CONF
AU  - Algarra, M.
AU  - Nešić, M. D.
AU  - Soto, J.
AU  - Urrutia, A.
AU  - Imas, J. J.
AU  - Dučić, T.
AU  - Petković, M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11818
AB  - The promising strategy for targeted cancer treatment is to employ light as an external activator of a drug accumulated in tumor tissue. This approach is so-called photodynamic therapy (PDT) and can be used for diagnostic purposes. A photosensitizer, molecular oxygen, and laser light are the three significant components of Type II PDT, and the mechanism is the catalysis of the production of reactive oxygen species that lead to the oxidative damage of cellular molecules inducing cancer cell death [1]. Fluorescent carbon dots (CDs), spherical nanoparticles with size < 10 nm that can function as bioimaging agents and photosensitizers, have demonstrated significant potential in cancer theranostics [2]. Here, we have created Nitrogen co-doped carbon dots (N-CDs) surface decorated with organometallics compound, based on the Ru complex (Ru@N-CDs) [3,4] that were active in inducing biomolecular changes in ovarian cancer cell line upon illumination. Upon illumination, the most significant structural changes occurred in ovarian cancer cells and were detected in the protein region; we postulate interference with signaling pathways involved in regulating cancer cell growth and tumor progression. However, the limitation of light is the depth of penetration through the tissues, which prevents significant therapeutic effects on deep tumors. A strategy to overcome this is to use optical fibers that have coatings fabricated from the N-CDs, thus developing a so-called lab-on fiber system. The light propagating through the fiber [4] can activate the overall coating on the optical fiber surface with the presence of Ru@N-CDs. We hypothesize that the activation by the light results in the locally increased ROS production combined with enhanced release of the Ru complex from the surface, similar to the Ru@TiO2 NCS [5]. Our preliminary results demonstrate a high potential of the lab-on-fiber system in therapy against ovarian cancer that can resist traditional chemotherapeutic approaches.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
T1  - Carbon dots nanoparticles as an effective gate for PDT
SP  - 121
EP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11818
ER  - 

@conference{
author = "Algarra, M. and Nešić, M. D. and Soto, J. and Urrutia, A. and Imas, J. J. and Dučić, T. and Petković, M.",
year = "2023",
abstract = "The promising strategy for targeted cancer treatment is to employ light as an external activator of a drug accumulated in tumor tissue. This approach is so-called photodynamic therapy (PDT) and can be used for diagnostic purposes. A photosensitizer, molecular oxygen, and laser light are the three significant components of Type II PDT, and the mechanism is the catalysis of the production of reactive oxygen species that lead to the oxidative damage of cellular molecules inducing cancer cell death [1]. Fluorescent carbon dots (CDs), spherical nanoparticles with size < 10 nm that can function as bioimaging agents and photosensitizers, have demonstrated significant potential in cancer theranostics [2]. Here, we have created Nitrogen co-doped carbon dots (N-CDs) surface decorated with organometallics compound, based on the Ru complex (Ru@N-CDs) [3,4] that were active in inducing biomolecular changes in ovarian cancer cell line upon illumination. Upon illumination, the most significant structural changes occurred in ovarian cancer cells and were detected in the protein region; we postulate interference with signaling pathways involved in regulating cancer cell growth and tumor progression. However, the limitation of light is the depth of penetration through the tissues, which prevents significant therapeutic effects on deep tumors. A strategy to overcome this is to use optical fibers that have coatings fabricated from the N-CDs, thus developing a so-called lab-on fiber system. The light propagating through the fiber [4] can activate the overall coating on the optical fiber surface with the presence of Ru@N-CDs. We hypothesize that the activation by the light results in the locally increased ROS production combined with enhanced release of the Ru complex from the surface, similar to the Ru@TiO2 NCS [5]. Our preliminary results demonstrate a high potential of the lab-on-fiber system in therapy against ovarian cancer that can resist traditional chemotherapeutic approaches.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade",
title = "Carbon dots nanoparticles as an effective gate for PDT",
pages = "121-121",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11818"
}

Algarra, M., Nešić, M. D., Soto, J., Urrutia, A., Imas, J. J., Dučić, T.,& Petković, M.. (2023). Carbon dots nanoparticles as an effective gate for PDT. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
Belgrade : Vinča Institute of Nuclear Sciences., 121-121.
https://hdl.handle.net/21.15107/rcub_vinar_11818

Algarra M, Nešić MD, Soto J, Urrutia A, Imas JJ, Dučić T, Petković M. Carbon dots nanoparticles as an effective gate for PDT. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade. 2023;:121-121.
https://hdl.handle.net/21.15107/rcub_vinar_11818 .

Algarra, M., Nešić, M. D., Soto, J., Urrutia, A., Imas, J. J., Dučić, T., Petković, M., "Carbon dots nanoparticles as an effective gate for PDT" in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade (2023):121-121,
https://hdl.handle.net/21.15107/rcub_vinar_11818 .