TY  - CONF
AU  - Nešić, M. D.
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Popović, I.
AU  - Soto, J.
AU  - Algarra, M.
AU  - Ralić, V.
AU  - Abu el Rub, Anamarija
AU  - Matijević, M.
AU  - Stepić, Milutin
AU  - Petković, M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11809
AB  - Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
T1  - Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11809
ER  - 

@conference{
author = "Nešić, M. D. and Filipović Tričković, Jelena and Valenta Šobot, Ana and Žakula, Jelena and Korićanac, Lela and Popović, I. and Soto, J. and Algarra, M. and Ralić, V. and Abu el Rub, Anamarija and Matijević, M. and Stepić, Milutin and Petković, M.",
year = "2023",
abstract = "Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade",
title = "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11809"
}

Nešić, M. D., Filipović Tričković, J., Valenta Šobot, A., Žakula, J., Korićanac, L., Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, A., Matijević, M., Stepić, M.,& Petković, M.. (2023). Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
Belgrade : Vinča Institute of Nuclear Sciences., 79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809

Nešić MD, Filipović Tričković J, Valenta Šobot A, Žakula J, Korićanac L, Popović I, Soto J, Algarra M, Ralić V, Abu el Rub A, Matijević M, Stepić M, Petković M. Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade. 2023;:79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

Nešić, M. D., Filipović Tričković, Jelena, Valenta Šobot, Ana, Žakula, Jelena, Korićanac, Lela, Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, Anamarija, Matijević, M., Stepić, Milutin, Petković, M., "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles" in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade (2023):79-79,
https://hdl.handle.net/21.15107/rcub_vinar_11809 .