TY  - JOUR
AU  - Kolaković, Ana
AU  - Bundalo, Maja
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13103
AB  - Background/Aim. The renin-angiotensin system (RAS) is linked to the development of atherosclerosis (As), including its initiation and progression. Besides the well-known angio-tensin-converting enzyme (ACE), two newer RAS family members are related to vascular remodeling - ACE2 as a homolog of ACE and collectrin [transmembrane protein 27 (TMEM27)] as a homolog of ACE2. Up to now, a limited number of studies have examined the expression of these RAS components in advanced carotid plaque (CP) tissue based on the sex of the patients and plaque phenotypes (PPs). There are two ultrasonographically defined PPs - the hypoechogenic plaque (HoP) and the hyperechogenic plaque (HerP) phenotype. The aim of the study was to investigate whether there was a correlation between the expression of RAS components in the CP and the sex and PPs of patients. Methods. We examined 74 patients with advanced CP who underwent carotid endarterectomy. The intraplaque expression of RAS components was determined with the real-time polymerase chain reaction, using the TaqMan® gene expression assays and Western blot. A two-way ANOVA followed by a post-hoc Tukey test was performed for the statistical analysis of results. Results. No interaction was recorded between the sex of the patients and PPs in influencing the relative expression of ACE and TMEM27 messenger RNA (mRNA) (p > 0.05). In 56.06% of plaque samples, no expression of ACE2 mRNA was detected. Among the plaques where ACE2 mRNA expression was detected, its expression level was higher in females with the HoP phenotype compared to females with the HerP phenotype (p < 0.001). In patients with the HoP phenotype, females had higher expression of ACE2 mRNA than males (p < 0.05). In the male study group, ACE protein levels were significantly lower in the HoP phenotype compared to the HerP phenotype (p < 0.001). Fe-males with the HoP phenotype had significantly higher ACE protein levels than males with the HoP phenotype (p < 0.0001). Conclusion. Our results revealed alterations in the expression levels of ACE and ACE2, at the mRNA and protein levels, in advanced carotid As. These alterations are impacted by sex and PP and may indicate a switch from the balanced RAS/ACE/ACE2 axis in the healthy blood vessel to the unbalanced axis in vascular remodeling due to As.
AB  - Renin-angiotenzin sistem (RAS) povezan je sa
razvojem ateroskleroze (As), uključujući njen nastanak i
progresiju. Pored dobro poznatog angiotenzinkonvertujućeg enzima (angiotensin-converting enzyme – ACE),
dva nova člana RAS familije povezana su sa
remodelovanjem zidova krvnih sudova – ACE2 kao
homolog ACE i kolektrin [transmembrane protein 27
(TMEM27)] kao homolog ACE2. Do sada je mali broj
studija ispitivao ekspresiju komponenti RAS sistema u tkivu
uznapredovalog karotidnog plaka (KP) u odnosu na pol
bolesnika i fenotip plaka (FP). Postoje dva tipa KP
definisana primenom ultrazvuka – fenotip hipoehogenog
plaka (HoP) i fenotip hiperehogenog plaka (HerP). Cilj rada
bio je da se ispita da li postoji korelacija između ekspresije
komponenti RAS u KP i pola i FP bolesnika. Metode.
Ispitano je 74 bolesnika sa uznapredovalim KP koji su bili
podvrgnuti operativnoj proceduri karotidne
endarterektomije. Ekspresija komponenti RAS sistema u
tkivu plaka utvrđena je lančanom reakcijom polimeraze u
realnom vremenu (real-time polymerase chain reaction)primenom eseja TaqMan® tehnologije i metode Western blota. Dvosmerna analiza varijanse i Tukey post-hoc test korišćen su za statističku obradu rezultata. Rezultati. Nije utvrđena
interakcija FP i pola bolesnika u uticaju na relativnu
ekspresiju informacione RNK (iRNK) za ACE i TMEM27
(p > 0,05). U 56,06% uzoraka plaka nije detektovana
ekspresija iRNK za ACE2. U plakovima u kojima je
detektovana ekspresija iRNK za ACE2, njen nivo bio je viši
kod žena sa HoP u poređenju sa ženama sa HerP (p <
0,001). U grupi bolesnika sa fenotipom HoP, žene su imale
viši nivo iRNK za ACE2 nego muškarci (p < 0,05). U grupi
muškaraca, nivoi ekspresije ACE proteina bili suznačajno
niži u fenotipu HoP u poređenju sa HerP (p < 0,001). Žene
sa fenotipom HoP imale su značajno viši nivo ACE
proteina u poređenju sa muškarcima sa HoP (p < 0,0001).
Zaključak. Naši rezultati pokazali su da postoje promene
nivoa ekspresije ACE i ACE2, na nivou proteina i iRNK u
uznapredovaloj karotidnoj As. Te promene zavise od pola i
FP i mogu ukazivati na to da balans ose RAS/ACE/ACE2
koji postoji u zdravom krvnom sudu postaje poremećen
tokom remodelovanja zida krvnog suda usled As.
T2  - Vojnosanitetski pregled
T1  - The expression of renin-angiotensin system components in human carotid plaque
T1  - Ekspresija komponenti renin-angiotenzin sistema u humanom karotidnom plaku
DO  - 10.2298/VSP221028014K
ER  - 

@article{
author = "Kolaković, Ana and Bundalo, Maja and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra and Živković, Maja",
year = "2024",
abstract = "Background/Aim. The renin-angiotensin system (RAS) is linked to the development of atherosclerosis (As), including its initiation and progression. Besides the well-known angio-tensin-converting enzyme (ACE), two newer RAS family members are related to vascular remodeling - ACE2 as a homolog of ACE and collectrin [transmembrane protein 27 (TMEM27)] as a homolog of ACE2. Up to now, a limited number of studies have examined the expression of these RAS components in advanced carotid plaque (CP) tissue based on the sex of the patients and plaque phenotypes (PPs). There are two ultrasonographically defined PPs - the hypoechogenic plaque (HoP) and the hyperechogenic plaque (HerP) phenotype. The aim of the study was to investigate whether there was a correlation between the expression of RAS components in the CP and the sex and PPs of patients. Methods. We examined 74 patients with advanced CP who underwent carotid endarterectomy. The intraplaque expression of RAS components was determined with the real-time polymerase chain reaction, using the TaqMan® gene expression assays and Western blot. A two-way ANOVA followed by a post-hoc Tukey test was performed for the statistical analysis of results. Results. No interaction was recorded between the sex of the patients and PPs in influencing the relative expression of ACE and TMEM27 messenger RNA (mRNA) (p > 0.05). In 56.06% of plaque samples, no expression of ACE2 mRNA was detected. Among the plaques where ACE2 mRNA expression was detected, its expression level was higher in females with the HoP phenotype compared to females with the HerP phenotype (p < 0.001). In patients with the HoP phenotype, females had higher expression of ACE2 mRNA than males (p < 0.05). In the male study group, ACE protein levels were significantly lower in the HoP phenotype compared to the HerP phenotype (p < 0.001). Fe-males with the HoP phenotype had significantly higher ACE protein levels than males with the HoP phenotype (p < 0.0001). Conclusion. Our results revealed alterations in the expression levels of ACE and ACE2, at the mRNA and protein levels, in advanced carotid As. These alterations are impacted by sex and PP and may indicate a switch from the balanced RAS/ACE/ACE2 axis in the healthy blood vessel to the unbalanced axis in vascular remodeling due to As., Renin-angiotenzin sistem (RAS) povezan je sa
razvojem ateroskleroze (As), uključujući njen nastanak i
progresiju. Pored dobro poznatog angiotenzinkonvertujućeg enzima (angiotensin-converting enzyme – ACE),
dva nova člana RAS familije povezana su sa
remodelovanjem zidova krvnih sudova – ACE2 kao
homolog ACE i kolektrin [transmembrane protein 27
(TMEM27)] kao homolog ACE2. Do sada je mali broj
studija ispitivao ekspresiju komponenti RAS sistema u tkivu
uznapredovalog karotidnog plaka (KP) u odnosu na pol
bolesnika i fenotip plaka (FP). Postoje dva tipa KP
definisana primenom ultrazvuka – fenotip hipoehogenog
plaka (HoP) i fenotip hiperehogenog plaka (HerP). Cilj rada
bio je da se ispita da li postoji korelacija između ekspresije
komponenti RAS u KP i pola i FP bolesnika. Metode.
Ispitano je 74 bolesnika sa uznapredovalim KP koji su bili
podvrgnuti operativnoj proceduri karotidne
endarterektomije. Ekspresija komponenti RAS sistema u
tkivu plaka utvrđena je lančanom reakcijom polimeraze u
realnom vremenu (real-time polymerase chain reaction)primenom eseja TaqMan® tehnologije i metode Western blota. Dvosmerna analiza varijanse i Tukey post-hoc test korišćen su za statističku obradu rezultata. Rezultati. Nije utvrđena
interakcija FP i pola bolesnika u uticaju na relativnu
ekspresiju informacione RNK (iRNK) za ACE i TMEM27
(p > 0,05). U 56,06% uzoraka plaka nije detektovana
ekspresija iRNK za ACE2. U plakovima u kojima je
detektovana ekspresija iRNK za ACE2, njen nivo bio je viši
kod žena sa HoP u poređenju sa ženama sa HerP (p <
0,001). U grupi bolesnika sa fenotipom HoP, žene su imale
viši nivo iRNK za ACE2 nego muškarci (p < 0,05). U grupi
muškaraca, nivoi ekspresije ACE proteina bili suznačajno
niži u fenotipu HoP u poređenju sa HerP (p < 0,001). Žene
sa fenotipom HoP imale su značajno viši nivo ACE
proteina u poređenju sa muškarcima sa HoP (p < 0,0001).
Zaključak. Naši rezultati pokazali su da postoje promene
nivoa ekspresije ACE i ACE2, na nivou proteina i iRNK u
uznapredovaloj karotidnoj As. Te promene zavise od pola i
FP i mogu ukazivati na to da balans ose RAS/ACE/ACE2
koji postoji u zdravom krvnom sudu postaje poremećen
tokom remodelovanja zida krvnog suda usled As.",
journal = "Vojnosanitetski pregled",
title = "The expression of renin-angiotensin system components in human carotid plaque, Ekspresija komponenti renin-angiotenzin sistema u humanom karotidnom plaku",
doi = "10.2298/VSP221028014K"
}

Kolaković, A., Bundalo, M., Đurić, T., Končar, I., Stanković, A.,& Živković, M.. (2024). The expression of renin-angiotensin system components in human carotid plaque. in Vojnosanitetski pregled.
https://doi.org/10.2298/VSP221028014K

Kolaković A, Bundalo M, Đurić T, Končar I, Stanković A, Živković M. The expression of renin-angiotensin system components in human carotid plaque. in Vojnosanitetski pregled. 2024;.
doi:10.2298/VSP221028014K .

Kolaković, Ana, Bundalo, Maja, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, Živković, Maja, "The expression of renin-angiotensin system components in human carotid plaque" in Vojnosanitetski pregled (2024),
https://doi.org/10.2298/VSP221028014K . .

TY  - CONF
AU  - Kolaković, Ana
AU  - Đurić, Tamara
AU  - Živković, Maja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12700
PB  - Kragujevac : Srpsko biološko društvo „Stevan Jakovljević“
C3  - Prva Konferencija Srpskog Biološkog Društva „Stevan Jakovljević“ : Program i izvodi saopštenja
T1  - Varijante u genima familije sirtuina: potencijal za buduća istraživanja u kardiovaskularnim bolestima
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12700
ER  - 

@conference{
author = "Kolaković, Ana and Đurić, Tamara and Živković, Maja",
year = "2023",
publisher = "Kragujevac : Srpsko biološko društvo „Stevan Jakovljević“",
journal = "Prva Konferencija Srpskog Biološkog Društva „Stevan Jakovljević“ : Program i izvodi saopštenja",
title = "Varijante u genima familije sirtuina: potencijal za buduća istraživanja u kardiovaskularnim bolestima",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12700"
}

Kolaković, A., Đurić, T.,& Živković, M.. (2023). Varijante u genima familije sirtuina: potencijal za buduća istraživanja u kardiovaskularnim bolestima. in Prva Konferencija Srpskog Biološkog Društva „Stevan Jakovljević“ : Program i izvodi saopštenja
Kragujevac : Srpsko biološko društvo „Stevan Jakovljević“..
https://hdl.handle.net/21.15107/rcub_vinar_12700

Kolaković A, Đurić T, Živković M. Varijante u genima familije sirtuina: potencijal za buduća istraživanja u kardiovaskularnim bolestima. in Prva Konferencija Srpskog Biološkog Društva „Stevan Jakovljević“ : Program i izvodi saopštenja. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_12700 .

Kolaković, Ana, Đurić, Tamara, Živković, Maja, "Varijante u genima familije sirtuina: potencijal za buduća istraživanja u kardiovaskularnim bolestima" in Prva Konferencija Srpskog Biološkog Društva „Stevan Jakovljević“ : Program i izvodi saopštenja (2023),
https://hdl.handle.net/21.15107/rcub_vinar_12700 .

TY  - JOUR
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Bošković, Maja
AU  - Dekleva, Milica
AU  - Stanković, Goran
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10617
AB  - Galectin-3 is encoded by LGALS-3, located in a unique haplotype block in Caucasians. According to the Tagger server, rs4040064, rs11628437, and rs7159490 cover 82% (r2 > 0.8) of the genetic variance of this HapBlock. Our aims were to examine the association of their haplotypes with first myocardial infarction (MI), changes in left ventricular echocardiographic parameters over time, and impact on plasma galectin-3 and LGALS-3 mRNA in peripheral blood mononuclear cells, both 6 months post-MI. The study group consisted of 546 MI patients and 323 controls. Gene expression was assessed in 92 patients and plasma galectin-3 in 189 patients. Rs4040064, rs11628437, rs7159490, and LGALS-3 mRNA expression were detected using TaqMan® technology. Plasma galectin-3 concentrations were determined by the ELISA method. We found that the TGC haplotype could have a protective effect against MI (adjusted OR 0.19 [0.05–0.72], p = 0.015) and that the GAC haplotype had significantly higher galectin-3 concentrations (48.3 [37.3–59.4] ng/mL vs. 18.9 [14.5–23.4] ng/mL, p < 0.0001), both in males and compared to the referent haplotype GGC. Higher plasma Gal-3 was also associated with higher NYHA class and systolic dysfunction. Our results suggest that variants tagging LGALS-3 HapBlock could reflect plasma Gal-3 levels 6 months post-MI and may have a potential protective effect against MI in men. Further replication, validation, and functional studies are needed.
T2  - Genes
T1  - Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event
VL  - 14
IS  - 1
SP  - 109
DO  - 10.3390/genes14010109
ER  - 

@article{
author = "Đorđević, Ana and Živković, Maja and Bošković, Maja and Dekleva, Milica and Stanković, Goran and Stanković, Aleksandra and Đurić, Tamara",
year = "2023",
abstract = "Galectin-3 is encoded by LGALS-3, located in a unique haplotype block in Caucasians. According to the Tagger server, rs4040064, rs11628437, and rs7159490 cover 82% (r2 > 0.8) of the genetic variance of this HapBlock. Our aims were to examine the association of their haplotypes with first myocardial infarction (MI), changes in left ventricular echocardiographic parameters over time, and impact on plasma galectin-3 and LGALS-3 mRNA in peripheral blood mononuclear cells, both 6 months post-MI. The study group consisted of 546 MI patients and 323 controls. Gene expression was assessed in 92 patients and plasma galectin-3 in 189 patients. Rs4040064, rs11628437, rs7159490, and LGALS-3 mRNA expression were detected using TaqMan® technology. Plasma galectin-3 concentrations were determined by the ELISA method. We found that the TGC haplotype could have a protective effect against MI (adjusted OR 0.19 [0.05–0.72], p = 0.015) and that the GAC haplotype had significantly higher galectin-3 concentrations (48.3 [37.3–59.4] ng/mL vs. 18.9 [14.5–23.4] ng/mL, p < 0.0001), both in males and compared to the referent haplotype GGC. Higher plasma Gal-3 was also associated with higher NYHA class and systolic dysfunction. Our results suggest that variants tagging LGALS-3 HapBlock could reflect plasma Gal-3 levels 6 months post-MI and may have a potential protective effect against MI in men. Further replication, validation, and functional studies are needed.",
journal = "Genes",
title = "Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event",
volume = "14",
number = "1",
pages = "109",
doi = "10.3390/genes14010109"
}

Đorđević, A., Živković, M., Bošković, M., Dekleva, M., Stanković, G., Stanković, A.,& Đurić, T.. (2023). Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event. in Genes, 14(1), 109.
https://doi.org/10.3390/genes14010109

Đorđević A, Živković M, Bošković M, Dekleva M, Stanković G, Stanković A, Đurić T. Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event. in Genes. 2023;14(1):109.
doi:10.3390/genes14010109 .

Đorđević, Ana, Živković, Maja, Bošković, Maja, Dekleva, Milica, Stanković, Goran, Stanković, Aleksandra, Đurić, Tamara, "Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event" in Genes, 14, no. 1 (2023):109,
https://doi.org/10.3390/genes14010109 . .

TY  - CONF
AU  - Dekleva, Milica
AU  - Martinović, M.
AU  - Stevanović, Angelina
AU  - Živković, Maja
AU  - Đorđević, Ana
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12854
AB  - Background: Right ventricular (RV) dysfunction is recognized as a cardinal prognostic marker in heart failure (HF) patients. Myocardial infarction (MI) is often followed by unrecognized RV dysfunction, which can be associated with worse outcome. It is recently shown that the ratio between TAPSE and PASP (RV/PA) may depict cardiopulmonary hemodynamics better than the two parameters alone. Aim: To evaluate the interactions between left ventricular (LV) and RV function in early phase of MI and to assess the prognostic significance of RV/PA coupling in patients with first MI during 5 years follow up. Methods: The prospective study included 144 patients with the first MI treated with the primary percutaneous coronary intervention (p PCI) who underwent Doppler echocardiography within 2±1 days of MI. LV function analysis included: LV ejection fraction (EF), ratio between early diastolic velocity and tissue annular velocity (E/e) and global longitudinal strain (GLS). RV function and RV-PA interaction was expressed as ratio between TAPSE and PASP. During the five-year follow-up, major cardiovascular events and especially hospitalization for HF were analyzed. Results: Progressive RV/PA uncoupling was associated with higher degree of LV impairment and dysfunction (EF p<0.001, E/e p=0.002, GLS p<0.001) and severity of mitral regurgitation (p=0.013). Lower baseline RV/PA coupling significantly reflects the frequency of hospitalizations for HF in the population of patients with first MI during five-year follow-up (0.62 v.s.0.51, p=0.021). After multivariate adjustment RV/PA remained an independent predictor of all major cardiac events (MACE) after five years (OR 14.0 [1.5–130.8], p=0.019). Conclusion: A lower baseline RV-PA coupling, reflecting a higher degree of LV-induced pulmonary hypertension and secondary RV-dysfunction, is associated with decline of LV function in early phase of MI, and is independently associated with worse prognosis after five years. The value of RV-PA ratio as an prognstic marker warrants further investigation.
C3  - European Heart Journal
T1  - Right ventricular-pulmonary artery coupling in the early stages of infarction could optimize patient risk stratification over five years
VL  - 44
IS  - Supplement 2
SP  - ehad655.067
DO  - 10.1093/eurheartj/ehad655.067
ER  - 

@conference{
author = "Dekleva, Milica and Martinović, M. and Stevanović, Angelina and Živković, Maja and Đorđević, Ana and Đurić, Tamara and Stanković, Aleksandra",
year = "2023",
abstract = "Background: Right ventricular (RV) dysfunction is recognized as a cardinal prognostic marker in heart failure (HF) patients. Myocardial infarction (MI) is often followed by unrecognized RV dysfunction, which can be associated with worse outcome. It is recently shown that the ratio between TAPSE and PASP (RV/PA) may depict cardiopulmonary hemodynamics better than the two parameters alone. Aim: To evaluate the interactions between left ventricular (LV) and RV function in early phase of MI and to assess the prognostic significance of RV/PA coupling in patients with first MI during 5 years follow up. Methods: The prospective study included 144 patients with the first MI treated with the primary percutaneous coronary intervention (p PCI) who underwent Doppler echocardiography within 2±1 days of MI. LV function analysis included: LV ejection fraction (EF), ratio between early diastolic velocity and tissue annular velocity (E/e) and global longitudinal strain (GLS). RV function and RV-PA interaction was expressed as ratio between TAPSE and PASP. During the five-year follow-up, major cardiovascular events and especially hospitalization for HF were analyzed. Results: Progressive RV/PA uncoupling was associated with higher degree of LV impairment and dysfunction (EF p<0.001, E/e p=0.002, GLS p<0.001) and severity of mitral regurgitation (p=0.013). Lower baseline RV/PA coupling significantly reflects the frequency of hospitalizations for HF in the population of patients with first MI during five-year follow-up (0.62 v.s.0.51, p=0.021). After multivariate adjustment RV/PA remained an independent predictor of all major cardiac events (MACE) after five years (OR 14.0 [1.5–130.8], p=0.019). Conclusion: A lower baseline RV-PA coupling, reflecting a higher degree of LV-induced pulmonary hypertension and secondary RV-dysfunction, is associated with decline of LV function in early phase of MI, and is independently associated with worse prognosis after five years. The value of RV-PA ratio as an prognstic marker warrants further investigation.",
journal = "European Heart Journal",
title = "Right ventricular-pulmonary artery coupling in the early stages of infarction could optimize patient risk stratification over five years",
volume = "44",
number = "Supplement 2",
pages = "ehad655.067",
doi = "10.1093/eurheartj/ehad655.067"
}

Dekleva, M., Martinović, M., Stevanović, A., Živković, M., Đorđević, A., Đurić, T.,& Stanković, A.. (2023). Right ventricular-pulmonary artery coupling in the early stages of infarction could optimize patient risk stratification over five years. in European Heart Journal, 44(Supplement 2), ehad655.067.
https://doi.org/10.1093/eurheartj/ehad655.067

Dekleva M, Martinović M, Stevanović A, Živković M, Đorđević A, Đurić T, Stanković A. Right ventricular-pulmonary artery coupling in the early stages of infarction could optimize patient risk stratification over five years. in European Heart Journal. 2023;44(Supplement 2):ehad655.067.
doi:10.1093/eurheartj/ehad655.067 .

Dekleva, Milica, Martinović, M., Stevanović, Angelina, Živković, Maja, Đorđević, Ana, Đurić, Tamara, Stanković, Aleksandra, "Right ventricular-pulmonary artery coupling in the early stages of infarction could optimize patient risk stratification over five years" in European Heart Journal, 44, no. Supplement 2 (2023):ehad655.067,
https://doi.org/10.1093/eurheartj/ehad655.067 . .

TY  - CONF
AU  - Kuveljić, Jovana
AU  - Životić, Ivan
AU  - Dekleva, Milica
AU  - Živković, Maja
AU  - Đurić, Tamara
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12648
AB  - Introduction: Myocardial infarction (MI) and consequential ischemia with cardiomyocyte loss are followed by left ventricular (LV) remodeling. LV remodeling is crucial process for cardiac function preservation, although when prolonged it can become maladaptive and lead to impaired systolic function and further cardiovascular complications. Echocardiographic parameters are used as a measure of LV structure and function. ADAM17 (a disintegrin and metalloprotease domain) and CDKN1A (cyclindependent kinase inhibitor 1A) have shown regulating role in DNA repair, inflammation, remodeling and fibrosis. The aim of this preliminary study was to investigate the potential effect of CDKN1 and ADAM17 mRNA in post MI heart remodeling. Methods: Sixty four patients with the first MI were prospectively followed-up 6 months after MI. Change (Δ) of echocardiographic parameters within 6 months was calculated as a difference between the value at 6-month follow-up and value at admission. Relative gene expression was detected using the TaqMan® technology. Statistical analyses were done by Statistica 8 software. Results: We have observed correlation between CDKN1A mRNA expression and change of LV enddiastolic diameter (ΔLVEDD, R=0.3, p=0.01) and LV end-systolic diameter (ΔLVESD, R=0.3, p=0.02), but not with LV ejection fraction and stroke volume. ADAM17 expression was not in correlation with analyzed parameters of LV remodeling. However, CDKN1A and ADAM17 mRNA expression in PBMC six months after MI were positively correlated (R=0.6, p<0.001). Conclusion: Preliminary resultssuggest that CDKN1 has a role in post MI LV remodeling, correlating with changes in echocardiographic parameters of LV structure. The validation on a larger sample size is required.
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts
T1  - Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction
SP  - 53
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12648
ER  - 

@conference{
author = "Kuveljić, Jovana and Životić, Ivan and Dekleva, Milica and Živković, Maja and Đurić, Tamara",
year = "2023",
abstract = "Introduction: Myocardial infarction (MI) and consequential ischemia with cardiomyocyte loss are followed by left ventricular (LV) remodeling. LV remodeling is crucial process for cardiac function preservation, although when prolonged it can become maladaptive and lead to impaired systolic function and further cardiovascular complications. Echocardiographic parameters are used as a measure of LV structure and function. ADAM17 (a disintegrin and metalloprotease domain) and CDKN1A (cyclindependent kinase inhibitor 1A) have shown regulating role in DNA repair, inflammation, remodeling and fibrosis. The aim of this preliminary study was to investigate the potential effect of CDKN1 and ADAM17 mRNA in post MI heart remodeling. Methods: Sixty four patients with the first MI were prospectively followed-up 6 months after MI. Change (Δ) of echocardiographic parameters within 6 months was calculated as a difference between the value at 6-month follow-up and value at admission. Relative gene expression was detected using the TaqMan® technology. Statistical analyses were done by Statistica 8 software. Results: We have observed correlation between CDKN1A mRNA expression and change of LV enddiastolic diameter (ΔLVEDD, R=0.3, p=0.01) and LV end-systolic diameter (ΔLVESD, R=0.3, p=0.02), but not with LV ejection fraction and stroke volume. ADAM17 expression was not in correlation with analyzed parameters of LV remodeling. However, CDKN1A and ADAM17 mRNA expression in PBMC six months after MI were positively correlated (R=0.6, p<0.001). Conclusion: Preliminary resultssuggest that CDKN1 has a role in post MI LV remodeling, correlating with changes in echocardiographic parameters of LV structure. The validation on a larger sample size is required.",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts",
title = "Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction",
pages = "53-53",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12648"
}

Kuveljić, J., Životić, I., Dekleva, M., Živković, M.,& Đurić, T.. (2023). Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts, 53-53.
https://hdl.handle.net/21.15107/rcub_vinar_12648

Kuveljić J, Životić I, Dekleva M, Živković M, Đurić T. Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts. 2023;:53-53.
https://hdl.handle.net/21.15107/rcub_vinar_12648 .

Kuveljić, Jovana, Životić, Ivan, Dekleva, Milica, Živković, Maja, Đurić, Tamara, "Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts (2023):53-53,
https://hdl.handle.net/21.15107/rcub_vinar_12648 .

TY  - JOUR
AU  - Đorđević, Ana D.
AU  - Živković, Maja
AU  - Končar, Igor
AU  - Stanković, Aleksandra
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10046
AB  - ObjectivesGalectin-3 affects a variety of biological processes. It is encoded by LGALS-3, located in unique haplotype block in Caucasians. Most of the studies regarding the gal-3 role in atherosclerosis are focused exclusively on protein/mRNA levels. Genetic analyses of LGALS-3 are scarce. We sought to thoroughly examine the genetic background of gal-3 and to analyze tag variants that cover more than 80% variability of the LGALS-3 containing hap-block in association with carotid plaque presence (CPP). According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T cover 82% (r2 > 0.8) of the genetic variance of this hap-block. Our aims were to investigate possible association of rs4040064, rs11628437 and rs7159490 haplotypes with CPP in patients with advanced carotid atherosclerosis (CA) and to analyze their possible effect on LGALS-3 mRNA expression in carotid plaques.Materials and methodsStudy group consisted of 468 patients and 296 controls. Rs4040064, rs11628437, rs7159490 and LGALS-3 mRNA expression were detected by TaqMan® technology.ResultsWe have found that haplotype TAC was associated with the cerebrovascular insult (CVI) occurrence (OR = 1.68, 95% CI = 1.09-2.58, p = 0.02), compared to the referent haplotype. OR was adjusted for hypertension, age and BMI. TAC also showed higher, but not statistically significant, LGALS-3 expression in carotid plaques.ConclusionsOur results suggest that rs4040064, rs11628437 and rs7159490 bear no association with CPP, neither they affect LGALS-3 mRNA in carotid plaques. However, we showed a significant association of haplotype TAC with the CVI occurrence in CA patients from Serbia. Replication and validation of our results are required.
T2  - Journal of Stroke and Cerebrovascular Diseases
T2  - Journal of Stroke and Cerebrovascular DiseasesJournal of Stroke and Cerebrovascular Diseases
T1  - Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis
VL  - 31
IS  - 1
SP  - 106212
DO  - 10.1016/j.jstrokecerebrovasdis.2021.106212
ER  - 

@article{
author = "Đorđević, Ana D. and Živković, Maja and Končar, Igor and Stanković, Aleksandra and Kuveljić, Jovana and Đurić, Tamara",
year = "2022",
abstract = "ObjectivesGalectin-3 affects a variety of biological processes. It is encoded by LGALS-3, located in unique haplotype block in Caucasians. Most of the studies regarding the gal-3 role in atherosclerosis are focused exclusively on protein/mRNA levels. Genetic analyses of LGALS-3 are scarce. We sought to thoroughly examine the genetic background of gal-3 and to analyze tag variants that cover more than 80% variability of the LGALS-3 containing hap-block in association with carotid plaque presence (CPP). According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T cover 82% (r2 > 0.8) of the genetic variance of this hap-block. Our aims were to investigate possible association of rs4040064, rs11628437 and rs7159490 haplotypes with CPP in patients with advanced carotid atherosclerosis (CA) and to analyze their possible effect on LGALS-3 mRNA expression in carotid plaques.Materials and methodsStudy group consisted of 468 patients and 296 controls. Rs4040064, rs11628437, rs7159490 and LGALS-3 mRNA expression were detected by TaqMan® technology.ResultsWe have found that haplotype TAC was associated with the cerebrovascular insult (CVI) occurrence (OR = 1.68, 95% CI = 1.09-2.58, p = 0.02), compared to the referent haplotype. OR was adjusted for hypertension, age and BMI. TAC also showed higher, but not statistically significant, LGALS-3 expression in carotid plaques.ConclusionsOur results suggest that rs4040064, rs11628437 and rs7159490 bear no association with CPP, neither they affect LGALS-3 mRNA in carotid plaques. However, we showed a significant association of haplotype TAC with the CVI occurrence in CA patients from Serbia. Replication and validation of our results are required.",
journal = "Journal of Stroke and Cerebrovascular Diseases, Journal of Stroke and Cerebrovascular DiseasesJournal of Stroke and Cerebrovascular Diseases",
title = "Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis",
volume = "31",
number = "1",
pages = "106212",
doi = "10.1016/j.jstrokecerebrovasdis.2021.106212"
}

Đorđević, A. D., Živković, M., Končar, I., Stanković, A., Kuveljić, J.,& Đurić, T.. (2022). Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases, 31(1), 106212.
https://doi.org/10.1016/j.jstrokecerebrovasdis.2021.106212

Đorđević AD, Živković M, Končar I, Stanković A, Kuveljić J, Đurić T. Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases. 2022;31(1):106212.
doi:10.1016/j.jstrokecerebrovasdis.2021.106212 .

Đorđević, Ana D., Živković, Maja, Končar, Igor, Stanković, Aleksandra, Kuveljić, Jovana, Đurić, Tamara, "Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis" in Journal of Stroke and Cerebrovascular Diseases, 31, no. 1 (2022):106212,
https://doi.org/10.1016/j.jstrokecerebrovasdis.2021.106212 . .

TY  - CONF
AU  - Dekleva, Milica
AU  - Đurić, Tamara
AU  - Đorđević, Ana
AU  - Soldatović, Ivan A.
AU  - Stanković, Aleksandra
AU  - Stevanović, Angelina
AU  - Živković, Maja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10710
AB  - Cardiac remodeling after the first myocardial infarction (MI) appears to be more successful in women than in men, but more frequently associated with heart failure (HF) development. Galectin-3 expression is upregulated in remodeling and failing myocardium and circulatory level is activated in hypertrophy, fibrosis and inflammation.This study aimed to investigate the potential role of sex differences in the following: risk factors, structural and functional left ventricle (LV) changes, coronary angiography, expression of Galectin-3 and it's circulating level for HF occurrence during 6 months in patients after first MI.The prospective study included patients with the first MI treated with the primary percutaneous coronary intervention (p PCI) who underwent Doppler echocardiography within 2±1 days of MI and after 6 months. Relative LGALS-3 mRNA expression in peripheral blood were detected by TaqMan® technology. Expression and concentration of Galectin-3 was obtained by ELISA method. Presence of HF was confirmed by clinical examination and Doppler echocardiography. Assessment of p PCI and description of coronary angiography was performed at the patient's admission time.The study included 137 men and 44 women, who were significantly older (57.8 vs. 54.4, p=0.034), with higher LDL cholesterol (3.54±0.93 vs. 4.03±1.27, p=0.027) without differences among angiographic characteristics and medications. In the acute phase of MI, the significantly lower indexed LV volumes were found in women compared to men (EDLVI: 58.3 vs. 49.6, p<0.001, ESLVI: 33.84 vs. 26.83, p<0.001), but the grade of LV remodeling (delta LVDVI, delta LVESVI) during 6 months and changes in LV ejection fraction (deltaLVEF) were similar (p=ns). Incidence of LV hypertrophy and HF development was significantly higher in women 70% vs. 44.6%, p=0.034, 37.5% vs.19.3%, p=0.02). Females have had a higher degree of LV diastolic dysfunction (DD) in the early and late phase after MI (p=0.038, p=0.027). There were significant correlations between grade of DD and level of Gal-3 expression (p=0.001). The relative expression of LGALS-3 mRNA in peripheral blood was higher in females (p=0.007) with upregulation of circulating Gal-3 in females (44.66 vs. 16.30, p<0.001) and in HF patients (31.1 vs. 21.2, p=0.025).Sex specific actions such as LV hypertrophy, diastolic dysfunction, upregulation of Galectin-3 expression and higher circulating level may explain more incidence of HF in female. Difference in model and determinants of HF between men and women can be important for further therapy including Gelectin-3 inhibition.Type of funding sources: None.
C3  - European Heart Journal
T1  - Sex related difference in heart failure development in patients after first myocardial infarction; role of galectine-3
VL  - 43
IS  - Supplement 2
SP  - ehac544.816
DO  - 10.1093/eurheartj/ehac544.816
ER  - 

@conference{
author = "Dekleva, Milica and Đurić, Tamara and Đorđević, Ana and Soldatović, Ivan A. and Stanković, Aleksandra and Stevanović, Angelina and Živković, Maja",
year = "2022",
abstract = "Cardiac remodeling after the first myocardial infarction (MI) appears to be more successful in women than in men, but more frequently associated with heart failure (HF) development. Galectin-3 expression is upregulated in remodeling and failing myocardium and circulatory level is activated in hypertrophy, fibrosis and inflammation.This study aimed to investigate the potential role of sex differences in the following: risk factors, structural and functional left ventricle (LV) changes, coronary angiography, expression of Galectin-3 and it's circulating level for HF occurrence during 6 months in patients after first MI.The prospective study included patients with the first MI treated with the primary percutaneous coronary intervention (p PCI) who underwent Doppler echocardiography within 2±1 days of MI and after 6 months. Relative LGALS-3 mRNA expression in peripheral blood were detected by TaqMan® technology. Expression and concentration of Galectin-3 was obtained by ELISA method. Presence of HF was confirmed by clinical examination and Doppler echocardiography. Assessment of p PCI and description of coronary angiography was performed at the patient's admission time.The study included 137 men and 44 women, who were significantly older (57.8 vs. 54.4, p=0.034), with higher LDL cholesterol (3.54±0.93 vs. 4.03±1.27, p=0.027) without differences among angiographic characteristics and medications. In the acute phase of MI, the significantly lower indexed LV volumes were found in women compared to men (EDLVI: 58.3 vs. 49.6, p<0.001, ESLVI: 33.84 vs. 26.83, p<0.001), but the grade of LV remodeling (delta LVDVI, delta LVESVI) during 6 months and changes in LV ejection fraction (deltaLVEF) were similar (p=ns). Incidence of LV hypertrophy and HF development was significantly higher in women 70% vs. 44.6%, p=0.034, 37.5% vs.19.3%, p=0.02). Females have had a higher degree of LV diastolic dysfunction (DD) in the early and late phase after MI (p=0.038, p=0.027). There were significant correlations between grade of DD and level of Gal-3 expression (p=0.001). The relative expression of LGALS-3 mRNA in peripheral blood was higher in females (p=0.007) with upregulation of circulating Gal-3 in females (44.66 vs. 16.30, p<0.001) and in HF patients (31.1 vs. 21.2, p=0.025).Sex specific actions such as LV hypertrophy, diastolic dysfunction, upregulation of Galectin-3 expression and higher circulating level may explain more incidence of HF in female. Difference in model and determinants of HF between men and women can be important for further therapy including Gelectin-3 inhibition.Type of funding sources: None.",
journal = "European Heart Journal",
title = "Sex related difference in heart failure development in patients after first myocardial infarction; role of galectine-3",
volume = "43",
number = "Supplement 2",
pages = "ehac544.816",
doi = "10.1093/eurheartj/ehac544.816"
}

Dekleva, M., Đurić, T., Đorđević, A., Soldatović, I. A., Stanković, A., Stevanović, A.,& Živković, M.. (2022). Sex related difference in heart failure development in patients after first myocardial infarction; role of galectine-3. in European Heart Journal, 43(Supplement 2), ehac544.816.
https://doi.org/10.1093/eurheartj/ehac544.816

Dekleva M, Đurić T, Đorđević A, Soldatović IA, Stanković A, Stevanović A, Živković M. Sex related difference in heart failure development in patients after first myocardial infarction; role of galectine-3. in European Heart Journal. 2022;43(Supplement 2):ehac544.816.
doi:10.1093/eurheartj/ehac544.816 .

Dekleva, Milica, Đurić, Tamara, Đorđević, Ana, Soldatović, Ivan A., Stanković, Aleksandra, Stevanović, Angelina, Živković, Maja, "Sex related difference in heart failure development in patients after first myocardial infarction; role of galectine-3" in European Heart Journal, 43, no. Supplement 2 (2022):ehac544.816,
https://doi.org/10.1093/eurheartj/ehac544.816 . .

TY  - JOUR
AU  - Đurić, Tamara
AU  - Kuveljić, Jovana
AU  - Đorđević, Ana
AU  - Dekleva, Milica
AU  - Stanković, Goran
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10382
AB  - Background Myocardial infarction (MI) leads to ischemia and afterward to left ventricular (LV) remodeling. Matrix metalloproteinase−1 (MMP1) and −3 (MMP3) belong to the family of endopeptidases and together they can dissolve most of the components of the extracellular matrix. MMP1 and MMP3 variants have been investigated solely in association with ischemic heart disease and LV dysfunction, but not in haplotype. The aims of this study were to investigate the association of haplotypes inferred from MMP1 rs1799750 (−1607 1G/2G; NC_000011.9:g.102670497del) and MMP3 rs35068180 (−1612 5A/6A; NC_000011.9:g.102715952dup) with MI and their effect on the change in echocardiographic parameters of LV structure and function in patients within 6 months after MI. Methods The study included 325 patients with the first MI and 283 healthy controls. Gene variants were detected by PCR-RFLP method. Parameters of LV structure and function were assessed by conventional 2D echocardiography, 3–5 days and 6 months after the first MI, on a subgroup of 160 patients. Haplotype analysis was performed with Thesias software. Results Haplotypes 2G-5A and 1G-6A were significantly and independently associated with MI compared with the reference haplotype 2G-6A (adjusted, p = 0.009 and p = 0.026, respectively). After Bonferroni correction for multiple testing, MMP1 and MMP3 haplotypes lost their association with the change in LV long diameter and stroke volume within 6 months after MI. Conclusion MMP1 and MMP3 haplotypes are strongly associated with MI. Further studies are needed to validate this result and to examine their association with echocardiographic parameters of LV structure and function after MI.
T2  - Molecular Genetics & Genomic Medicine
T1  - Association of MMP1 and MMP3 haplotypes with myocardial infarction and echocardiographic parameters of the left ventricle
VL  - 10
IS  - 9
SP  - e2022
DO  - 10.1002/mgg3.2022
ER  - 

@article{
author = "Đurić, Tamara and Kuveljić, Jovana and Đorđević, Ana and Dekleva, Milica and Stanković, Goran and Stanković, Aleksandra and Živković, Maja",
year = "2022",
abstract = "Background Myocardial infarction (MI) leads to ischemia and afterward to left ventricular (LV) remodeling. Matrix metalloproteinase−1 (MMP1) and −3 (MMP3) belong to the family of endopeptidases and together they can dissolve most of the components of the extracellular matrix. MMP1 and MMP3 variants have been investigated solely in association with ischemic heart disease and LV dysfunction, but not in haplotype. The aims of this study were to investigate the association of haplotypes inferred from MMP1 rs1799750 (−1607 1G/2G; NC_000011.9:g.102670497del) and MMP3 rs35068180 (−1612 5A/6A; NC_000011.9:g.102715952dup) with MI and their effect on the change in echocardiographic parameters of LV structure and function in patients within 6 months after MI. Methods The study included 325 patients with the first MI and 283 healthy controls. Gene variants were detected by PCR-RFLP method. Parameters of LV structure and function were assessed by conventional 2D echocardiography, 3–5 days and 6 months after the first MI, on a subgroup of 160 patients. Haplotype analysis was performed with Thesias software. Results Haplotypes 2G-5A and 1G-6A were significantly and independently associated with MI compared with the reference haplotype 2G-6A (adjusted, p = 0.009 and p = 0.026, respectively). After Bonferroni correction for multiple testing, MMP1 and MMP3 haplotypes lost their association with the change in LV long diameter and stroke volume within 6 months after MI. Conclusion MMP1 and MMP3 haplotypes are strongly associated with MI. Further studies are needed to validate this result and to examine their association with echocardiographic parameters of LV structure and function after MI.",
journal = "Molecular Genetics & Genomic Medicine",
title = "Association of MMP1 and MMP3 haplotypes with myocardial infarction and echocardiographic parameters of the left ventricle",
volume = "10",
number = "9",
pages = "e2022",
doi = "10.1002/mgg3.2022"
}

Đurić, T., Kuveljić, J., Đorđević, A., Dekleva, M., Stanković, G., Stanković, A.,& Živković, M.. (2022). Association of MMP1 and MMP3 haplotypes with myocardial infarction and echocardiographic parameters of the left ventricle. in Molecular Genetics & Genomic Medicine, 10(9), e2022.
https://doi.org/10.1002/mgg3.2022

Đurić T, Kuveljić J, Đorđević A, Dekleva M, Stanković G, Stanković A, Živković M. Association of MMP1 and MMP3 haplotypes with myocardial infarction and echocardiographic parameters of the left ventricle. in Molecular Genetics & Genomic Medicine. 2022;10(9):e2022.
doi:10.1002/mgg3.2022 .

Đurić, Tamara, Kuveljić, Jovana, Đorđević, Ana, Dekleva, Milica, Stanković, Goran, Stanković, Aleksandra, Živković, Maja, "Association of MMP1 and MMP3 haplotypes with myocardial infarction and echocardiographic parameters of the left ventricle" in Molecular Genetics & Genomic Medicine, 10, no. 9 (2022):e2022,
https://doi.org/10.1002/mgg3.2022 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Bubić, Maja
AU  - Kolaković, Ana
AU  - Dekleva, Milica
AU  - Stanković, Goran
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9842
AB  - Glutathione S-transferases (GSTs) are the family of enzymes involved in the second line of defense against oxidative stress (OS). The lack of GSTT1/GSTM1 enzyme quantity or activity, due to the presence of homozygous deletion compromises antioxidative defense resulting in OS. OS is the critical mechanism in the pathophysiology of atherosclerosis, coronary artery disease, and myocardial infarction (MI). The increase in reactive oxygen species together with the process of apoptosis plays a role in left ventricular remodeling (LVR) after MI. The associations of GSTT1 and GSTM1 gene polymorphisms with the risk of MI are inconsistent. The aim was to analyze the association of GSTT1/GSTM1 null genotypes with first MI and LVR 8 months after the MI. The study involved 330 controls and 438 consecutive patients with symptoms and signs of first MI. The subgroup of 150 MI patients was prospectively followed up for 6 months. Evidence of maladaptive LVR was obtained by 2D Doppler echocardiography 3-5 days and 6 months after the MI. A multiplex polymerase chain reaction was used to detect the deletion in GSTT1 and GSTM1 genes. GSTM1 null genotype was significantly and independently associated with first MI (adjusted OR = 1.45 95% CI 1.03-2.03, p = 0.03). Association of double null genotypes with maladaptive LVR in patients 6 months after the first MI was no longer significant after adjustment for factors that differed significantly between patients with and without maladaptive LVR. This study demonstrated the association of GSTM1 null genotypes with the risk of MI in the Serbian population.
T2  - Free Radical Research
T1  - The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction
VL  - 55
IS  - 3
SP  - 267
EP  - 274
DO  - 10.1080/10715762.2021.1931166
ER  - 

@article{
author = "Živković, Maja and Bubić, Maja and Kolaković, Ana and Dekleva, Milica and Stanković, Goran and Stanković, Aleksandra and Đurić, Tamara",
year = "2021",
abstract = "Glutathione S-transferases (GSTs) are the family of enzymes involved in the second line of defense against oxidative stress (OS). The lack of GSTT1/GSTM1 enzyme quantity or activity, due to the presence of homozygous deletion compromises antioxidative defense resulting in OS. OS is the critical mechanism in the pathophysiology of atherosclerosis, coronary artery disease, and myocardial infarction (MI). The increase in reactive oxygen species together with the process of apoptosis plays a role in left ventricular remodeling (LVR) after MI. The associations of GSTT1 and GSTM1 gene polymorphisms with the risk of MI are inconsistent. The aim was to analyze the association of GSTT1/GSTM1 null genotypes with first MI and LVR 8 months after the MI. The study involved 330 controls and 438 consecutive patients with symptoms and signs of first MI. The subgroup of 150 MI patients was prospectively followed up for 6 months. Evidence of maladaptive LVR was obtained by 2D Doppler echocardiography 3-5 days and 6 months after the MI. A multiplex polymerase chain reaction was used to detect the deletion in GSTT1 and GSTM1 genes. GSTM1 null genotype was significantly and independently associated with first MI (adjusted OR = 1.45 95% CI 1.03-2.03, p = 0.03). Association of double null genotypes with maladaptive LVR in patients 6 months after the first MI was no longer significant after adjustment for factors that differed significantly between patients with and without maladaptive LVR. This study demonstrated the association of GSTM1 null genotypes with the risk of MI in the Serbian population.",
journal = "Free Radical Research",
title = "The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction",
volume = "55",
number = "3",
pages = "267-274",
doi = "10.1080/10715762.2021.1931166"
}

Živković, M., Bubić, M., Kolaković, A., Dekleva, M., Stanković, G., Stanković, A.,& Đurić, T.. (2021). The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction. in Free Radical Research, 55(3), 267-274.
https://doi.org/10.1080/10715762.2021.1931166

Živković M, Bubić M, Kolaković A, Dekleva M, Stanković G, Stanković A, Đurić T. The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction. in Free Radical Research. 2021;55(3):267-274.
doi:10.1080/10715762.2021.1931166 .

Živković, Maja, Bubić, Maja, Kolaković, Ana, Dekleva, Milica, Stanković, Goran, Stanković, Aleksandra, Đurić, Tamara, "The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction" in Free Radical Research, 55, no. 3 (2021):267-274,
https://doi.org/10.1080/10715762.2021.1931166 . .

TY  - CONF
AU  - Đorđević, Ana M.
AU  - Dekleva, Milica
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12729
AB  - After myocardial infarction (MI) and consequential ischemia, the heart undergoes a set of geometric and functional changes. In the early days after injury, these changes, defined as cardiac remodeling, are the powerful factor that preserves cardiac function and promotes survival. However, it may continue for months after MI and eventually lead to adverse remodeling with impaired systolic function and reduced myocardial contractility and further cardiovascular complications, such as heart failure (HF). Left ventricular (LV) ejection fraction (EF) is widely used as an index of systolic function in cardiac patients. However, global myocardial strain has been found to be superior to the conventional parameters, such as LVEF, in terms of assessment of cardiac performance after MI. Galectin-3 (gal-3) is a multifunctional protein involved in a variety of physiological and pathological processes, affecting the entire cardiovascular continuum of MI. Gal-3 is encoded by a LGALS-3 gene, located in a unique, 300 kb long haplotype block in Caucasians. Gal-3 serum level has been approved as a diagnostic marker for risk stratification and prognosis evaluation of HF patients according to the ACC/AHA/HFSA Guideline for the management of HF. The purpose of the present prospective study was to analyze the possible association of tag genetic variants of the haplotype block containing LGALS-3 with changes in cardiac parameters, LVEF and global radial strain (GRS), within 6 months post-MI. The study enrolled 120 patients with first acute MI that were prospectively followed-up 6 months after MI. According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T variants cover 82% (r2>0.8) of phenotypic variance of the aforementioned haplotype block. Tag variants were detected and genotyped by commercially available assays for allelic discrimination. Echocardiography examinations were performed at admission and 6 months post-MI. Change (Δ) of cardiac parameters was calculated as a difference between the value at 6-month follow-up and baseline value (at admission). The referent haplotype is set by the software for carrying haplotype association analysis and represents the most frequent haplotype in the studied groups. Bonferroni correction for multiple testing was performed and p values <0.025 were considered as statistically significant.We found that, compared to the reference GGC haplotype, GAT haplotype had significantly higher expected phenotypic mean [95% CI] of ΔGRS (3.77 [1.28 - 6.25] vs. −5.34 [−12.69 - 2.01], respectively, p=0.025) and ΔLVEF (0.84 [−1.88 - 3.56] vs. −12.91 [−17.30 - −8.53], respectively, p=0.00001), in the direction of decrease of GRS and LVEF 6 months after MI in patients bearing GAT haplotype. Our findings suggest that GAT haplotype bears the risk for diminished LV transmural contractility and radial systolic function: In order to reach a definitive conclusion, our exploratory results should be further validated on a larger sample
C3  - European Journal of Hear Failure
T1  - LGALS-3 containing haplotype block tag variants in association with cardiac parameters changes within six months after the first acute myocardial infarction
VL  - 23
IS  - Suppl. S2
SP  - 311
EP  - 311
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12729
ER  - 

@conference{
author = "Đorđević, Ana M. and Dekleva, Milica and Živković, Maja and Stanković, Aleksandra and Kuveljić, Jovana and Đurić, Tamara",
year = "2021",
abstract = "After myocardial infarction (MI) and consequential ischemia, the heart undergoes a set of geometric and functional changes. In the early days after injury, these changes, defined as cardiac remodeling, are the powerful factor that preserves cardiac function and promotes survival. However, it may continue for months after MI and eventually lead to adverse remodeling with impaired systolic function and reduced myocardial contractility and further cardiovascular complications, such as heart failure (HF). Left ventricular (LV) ejection fraction (EF) is widely used as an index of systolic function in cardiac patients. However, global myocardial strain has been found to be superior to the conventional parameters, such as LVEF, in terms of assessment of cardiac performance after MI. Galectin-3 (gal-3) is a multifunctional protein involved in a variety of physiological and pathological processes, affecting the entire cardiovascular continuum of MI. Gal-3 is encoded by a LGALS-3 gene, located in a unique, 300 kb long haplotype block in Caucasians. Gal-3 serum level has been approved as a diagnostic marker for risk stratification and prognosis evaluation of HF patients according to the ACC/AHA/HFSA Guideline for the management of HF. The purpose of the present prospective study was to analyze the possible association of tag genetic variants of the haplotype block containing LGALS-3 with changes in cardiac parameters, LVEF and global radial strain (GRS), within 6 months post-MI. The study enrolled 120 patients with first acute MI that were prospectively followed-up 6 months after MI. According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T variants cover 82% (r2>0.8) of phenotypic variance of the aforementioned haplotype block. Tag variants were detected and genotyped by commercially available assays for allelic discrimination. Echocardiography examinations were performed at admission and 6 months post-MI. Change (Δ) of cardiac parameters was calculated as a difference between the value at 6-month follow-up and baseline value (at admission). The referent haplotype is set by the software for carrying haplotype association analysis and represents the most frequent haplotype in the studied groups. Bonferroni correction for multiple testing was performed and p values <0.025 were considered as statistically significant.We found that, compared to the reference GGC haplotype, GAT haplotype had significantly higher expected phenotypic mean [95% CI] of ΔGRS (3.77 [1.28 - 6.25] vs. −5.34 [−12.69 - 2.01], respectively, p=0.025) and ΔLVEF (0.84 [−1.88 - 3.56] vs. −12.91 [−17.30 - −8.53], respectively, p=0.00001), in the direction of decrease of GRS and LVEF 6 months after MI in patients bearing GAT haplotype. Our findings suggest that GAT haplotype bears the risk for diminished LV transmural contractility and radial systolic function: In order to reach a definitive conclusion, our exploratory results should be further validated on a larger sample",
journal = "European Journal of Hear Failure",
title = "LGALS-3 containing haplotype block tag variants in association with cardiac parameters changes within six months after the first acute myocardial infarction",
volume = "23",
number = "Suppl. S2",
pages = "311-311",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12729"
}

Đorđević, A. M., Dekleva, M., Živković, M., Stanković, A., Kuveljić, J.,& Đurić, T.. (2021). LGALS-3 containing haplotype block tag variants in association with cardiac parameters changes within six months after the first acute myocardial infarction. in European Journal of Hear Failure, 23(Suppl. S2), 311-311.
https://hdl.handle.net/21.15107/rcub_vinar_12729

Đorđević AM, Dekleva M, Živković M, Stanković A, Kuveljić J, Đurić T. LGALS-3 containing haplotype block tag variants in association with cardiac parameters changes within six months after the first acute myocardial infarction. in European Journal of Hear Failure. 2021;23(Suppl. S2):311-311.
https://hdl.handle.net/21.15107/rcub_vinar_12729 .

Đorđević, Ana M., Dekleva, Milica, Živković, Maja, Stanković, Aleksandra, Kuveljić, Jovana, Đurić, Tamara, "LGALS-3 containing haplotype block tag variants in association with cardiac parameters changes within six months after the first acute myocardial infarction" in European Journal of Hear Failure, 23, no. Suppl. S2 (2021):311-311,
https://hdl.handle.net/21.15107/rcub_vinar_12729 .

TY  - JOUR
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
AU  - Stanković, Goran
AU  - Dekleva, Milica
AU  - Stanković, Aleksandra
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9541
AB  - Background: Myocardial infarction (MI) and underlining atherosclerosis are the main causes of death worldwide. Phosphatase and actin regulator 1 (PHACTR1) variants have been associated with early onset MI, coronary artery disease and carotid dissection. PHACTR1 mRNA expression has been detected in tissues and cells related to atherosclerosis. Nonetheless, the true effect of PHACTR1 on vascular diseases is still unknown. Our aim was to examine the association of PHACTR1 intronic variants, rs9349379, rs2026458 and rs2876300, with MI and multi-vessel disease (MVD) and to assess their effect on PHACTR1 and EDN1 mRNA expression in PBMCs of patients six months after MI. Methods: The study enrolled 537 patients with the first MI and 310 controls. Gene expression was assessed in 74 patients six months after MI and 37 healthy controls. Rs9349379, rs2026458, rs2876300 and relative mRNA expressions were detected by TaqMan® technology. Results: The significant association between PHACTR1 variants and MI was not found, either individually or in haplotype. A higher frequency of rs2876300G-allele in MVD was rendered not significant after Bonferroni correction. PHACTR1 mRNA was significantly increased in PBMCs of patients six months after MI compared to controls (p = 0.02). Patients that carry ACG haplotype have increased PHACTR1 mRNA expression in PBMCs (p = 0.04). There was no effect of PHACTR1 variants on EDN1 mRNA expression. Conclusion: Our findings suggest that PHACTR1 intronic variants may have a role in severity and progression of coronary atherosclerosis. Future research is needed to clarify the mechanism underlying the role of PHACTR1 in coronary atherosclerosis and MI. © 2021 Elsevier B.V.
T2  - Gene
T1  - Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs
VL  - 775
SP  - 145428
DO  - 10.1016/j.gene.2021.145428
ER  - 

@article{
author = "Kuveljić, Jovana and Đurić, Tamara and Stanković, Goran and Dekleva, Milica and Stanković, Aleksandra and Alavantić, Dragan and Živković, Maja",
year = "2021",
abstract = "Background: Myocardial infarction (MI) and underlining atherosclerosis are the main causes of death worldwide. Phosphatase and actin regulator 1 (PHACTR1) variants have been associated with early onset MI, coronary artery disease and carotid dissection. PHACTR1 mRNA expression has been detected in tissues and cells related to atherosclerosis. Nonetheless, the true effect of PHACTR1 on vascular diseases is still unknown. Our aim was to examine the association of PHACTR1 intronic variants, rs9349379, rs2026458 and rs2876300, with MI and multi-vessel disease (MVD) and to assess their effect on PHACTR1 and EDN1 mRNA expression in PBMCs of patients six months after MI. Methods: The study enrolled 537 patients with the first MI and 310 controls. Gene expression was assessed in 74 patients six months after MI and 37 healthy controls. Rs9349379, rs2026458, rs2876300 and relative mRNA expressions were detected by TaqMan® technology. Results: The significant association between PHACTR1 variants and MI was not found, either individually or in haplotype. A higher frequency of rs2876300G-allele in MVD was rendered not significant after Bonferroni correction. PHACTR1 mRNA was significantly increased in PBMCs of patients six months after MI compared to controls (p = 0.02). Patients that carry ACG haplotype have increased PHACTR1 mRNA expression in PBMCs (p = 0.04). There was no effect of PHACTR1 variants on EDN1 mRNA expression. Conclusion: Our findings suggest that PHACTR1 intronic variants may have a role in severity and progression of coronary atherosclerosis. Future research is needed to clarify the mechanism underlying the role of PHACTR1 in coronary atherosclerosis and MI. © 2021 Elsevier B.V.",
journal = "Gene",
title = "Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs",
volume = "775",
pages = "145428",
doi = "10.1016/j.gene.2021.145428"
}

Kuveljić, J., Đurić, T., Stanković, G., Dekleva, M., Stanković, A., Alavantić, D.,& Živković, M.. (2021). Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs. in Gene, 775, 145428.
https://doi.org/10.1016/j.gene.2021.145428

Kuveljić J, Đurić T, Stanković G, Dekleva M, Stanković A, Alavantić D, Živković M. Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs. in Gene. 2021;775:145428.
doi:10.1016/j.gene.2021.145428 .

Kuveljić, Jovana, Đurić, Tamara, Stanković, Goran, Dekleva, Milica, Stanković, Aleksandra, Alavantić, Dragan, Živković, Maja, "Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs" in Gene, 775 (2021):145428,
https://doi.org/10.1016/j.gene.2021.145428 . .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Zec, Manja
AU  - Đurić, Tamara
AU  - Životić, Ivan
AU  - Kuveljić, Jovana
AU  - Kolaković, Ana
AU  - Kolić, Ivana
AU  - Đorđević, Ana
AU  - Glibetić, Marija
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8683
AB  - Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.
T2  - Nutrients
T1  - The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
VL  - 12
IS  - 5
SP  - 1484
DO  - 10.3390/nu12051484
ER  - 

@article{
author = "Stojković, Ljiljana S. and Jovanović, Ivan G. and Živković, Maja and Zec, Manja and Đurić, Tamara and Životić, Ivan and Kuveljić, Jovana and Kolaković, Ana and Kolić, Ivana and Đorđević, Ana and Glibetić, Marija and Alavantić, Dragan and Stanković, Aleksandra",
year = "2020",
abstract = "Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.",
journal = "Nutrients",
title = "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk",
volume = "12",
number = "5",
pages = "1484",
doi = "10.3390/nu12051484"
}

Stojković, L. S., Jovanović, I. G., Živković, M., Zec, M., Đurić, T., Životić, I., Kuveljić, J., Kolaković, A., Kolić, I., Đorđević, A., Glibetić, M., Alavantić, D.,& Stanković, A.. (2020). The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients, 12(5), 1484.
https://doi.org/10.3390/nu12051484

Stojković LS, Jovanović IG, Živković M, Zec M, Đurić T, Životić I, Kuveljić J, Kolaković A, Kolić I, Đorđević A, Glibetić M, Alavantić D, Stanković A. The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients. 2020;12(5):1484.
doi:10.3390/nu12051484 .

Stojković, Ljiljana S., Jovanović, Ivan G., Živković, Maja, Zec, Manja, Đurić, Tamara, Životić, Ivan, Kuveljić, Jovana, Kolaković, Ana, Kolić, Ivana, Đorđević, Ana, Glibetić, Marija, Alavantić, Dragan, Stanković, Aleksandra, "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk" in Nutrients, 12, no. 5 (2020):1484,
https://doi.org/10.3390/nu12051484 . .

TY  - JOUR
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Stojković, Ljiljana S.
AU  - Ješić, Snežana
AU  - Stanković, Aleksandra
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8684
AB  - Objectives/Hypothesis: To establish comprehensive transcriptomic profiles of cholesteatoma perimatrix tissue and granulation tissue from chronic otitis media (COM) that did not develop cholesteatoma, which can indicate molecular pathways involved in the cholesteatoma perimatrix pathology and invasiveness. Study Design: Retrospective Case Series. Methods: Transcriptome data were obtained from cholesteatoma perimatrix tissue and COM granulation tissue by an Illumina iScan microarray. Differentially expressed genes (DEGs) were subsequently analyzed using both bioinformatical functional annotation and network analysis. Expression of candidate genes (MMP9 and LCN2) was validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) on a larger group of samples. Results: Analysis of the transcriptome led to the identification of 169 differentially expressed genes between investigated tissues. Bioinformatic analysis suggested that most significant biological processes involving DEGs were previously described in cholesteatoma pathology. Network analysis identified ERBB2, TFAP2A, and TP63 as major hubs of the DEGs molecular network. Furthermore, it was observed that the cellular component most significantly enriched in DEGs was extracellular space containing 47 DEGs. Using qRT-PCR, it was confirmed that mRNA levels of the major extracellular hub (MMP9) are increased, whereas its interacting molecule (LCN2) mRNA levels were decreased in cholesteatoma perimatrix tissue compared to COM granulation tissue. Conclusions: The current study approach offers an overall look at molecular mechanisms that describe the cholesteatoma entity by focusing exclusively on the perimatrix processes in comparison to COM granulation tissue. The observed differences in gene expression between cholesteatoma perimatrix and COM granulation tissue could suggest novel markers potentially influenced by the perimatrix–matrix molecular interplay, which is not present in COM without cholesteatoma. Level of Evidence: NA. Laryngoscope, 130:E220–E227, 2020. © 2019 The American Laryngological, Rhinological and Otological Society, Inc.
T2  - The Laryngoscope
T1  - Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature
VL  - 130
IS  - 4
SP  - E220
EP  - E227
DO  - 10.1002/lary.28084
ER  - 

@article{
author = "Jovanović, Ivan G. and Živković, Maja and Đurić, Tamara and Stojković, Ljiljana S. and Ješić, Snežana and Stanković, Aleksandra",
year = "2020",
abstract = "Objectives/Hypothesis: To establish comprehensive transcriptomic profiles of cholesteatoma perimatrix tissue and granulation tissue from chronic otitis media (COM) that did not develop cholesteatoma, which can indicate molecular pathways involved in the cholesteatoma perimatrix pathology and invasiveness. Study Design: Retrospective Case Series. Methods: Transcriptome data were obtained from cholesteatoma perimatrix tissue and COM granulation tissue by an Illumina iScan microarray. Differentially expressed genes (DEGs) were subsequently analyzed using both bioinformatical functional annotation and network analysis. Expression of candidate genes (MMP9 and LCN2) was validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) on a larger group of samples. Results: Analysis of the transcriptome led to the identification of 169 differentially expressed genes between investigated tissues. Bioinformatic analysis suggested that most significant biological processes involving DEGs were previously described in cholesteatoma pathology. Network analysis identified ERBB2, TFAP2A, and TP63 as major hubs of the DEGs molecular network. Furthermore, it was observed that the cellular component most significantly enriched in DEGs was extracellular space containing 47 DEGs. Using qRT-PCR, it was confirmed that mRNA levels of the major extracellular hub (MMP9) are increased, whereas its interacting molecule (LCN2) mRNA levels were decreased in cholesteatoma perimatrix tissue compared to COM granulation tissue. Conclusions: The current study approach offers an overall look at molecular mechanisms that describe the cholesteatoma entity by focusing exclusively on the perimatrix processes in comparison to COM granulation tissue. The observed differences in gene expression between cholesteatoma perimatrix and COM granulation tissue could suggest novel markers potentially influenced by the perimatrix–matrix molecular interplay, which is not present in COM without cholesteatoma. Level of Evidence: NA. Laryngoscope, 130:E220–E227, 2020. © 2019 The American Laryngological, Rhinological and Otological Society, Inc.",
journal = "The Laryngoscope",
title = "Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature",
volume = "130",
number = "4",
pages = "E220-E227",
doi = "10.1002/lary.28084"
}

Jovanović, I. G., Živković, M., Đurić, T., Stojković, L. S., Ješić, S.,& Stanković, A.. (2020). Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature. in The Laryngoscope, 130(4), E220-E227.
https://doi.org/10.1002/lary.28084

Jovanović IG, Živković M, Đurić T, Stojković LS, Ješić S, Stanković A. Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature. in The Laryngoscope. 2020;130(4):E220-E227.
doi:10.1002/lary.28084 .

Jovanović, Ivan G., Živković, Maja, Đurić, Tamara, Stojković, Ljiljana S., Ješić, Snežana, Stanković, Aleksandra, "Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature" in The Laryngoscope, 130, no. 4 (2020):E220-E227,
https://doi.org/10.1002/lary.28084 . .

TY  - JOUR
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Končar, Igor
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8348
AB  - Background: Carotid plaque is a hallmark of advanced carotid atherosclerosis and there is evidence of phosphatase and actin regulator 1 (PHACTR1) involvement in the processes that lead to atherosclerosis. PHACTR1 intronic variants have been associated with coronary artery disease and carotid dissection. Up to date the PHACTR1 haplotypes were not investigated in association with carotid plaque presence (CPP). So, the aims of this study were to investigate possible association of PHACTR1 haplotypes inferred from the intronic variants rs9349379, rs2026458 and rs2876300 with CPP in patients with advanced carotid atherosclerosis and to analyze their possible effect on PHACTR1 relative mRNA expression in carotid plaque tissue specimens. Methods: The study group consisted of 501 patients with evidence of carotid plaque presence admitted for carotid endarterectomy and 310 healthy controls. PHACTR1 rs9349379, rs2026458, rs2876300 and relative mRNA expression were detected by TaqMan® technology. Results: We have found significant and independent association of haplotype ACA with the CPP, compared to the referent haplotype GTA (adjusted OR = 1.54 95% CI = 1.07–2.21, p = 0.02). The OR was adjusted for gender, age, BMI, hypertension and total cholesterol. The relative expression of PHACTR1 mRNA in carotid plaque tissue proved to be significantly higher in carriers of the ACG haplotype compared to the referent haplotype GTA (p = 0.03). Conclusion: Our results suggest that PHACTR1 haplotypes inferred from the variants rs9349379, rs2026458 and rs2876300 affect PHACTR1 mRNA and bear the risk for CPP in patients with advanced carotid atherosclerosis. Further replication and validation studies are inevitable. © 2019 Elsevier B.V.
T2  - Gene
T1  - PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue
VL  - 710
SP  - 273
EP  - 278
DO  - 10.1016/j.gene.2019.06.020
ER  - 

@article{
author = "Kuveljić, Jovana and Đurić, Tamara and Stanković, Aleksandra and Končar, Igor and Alavantić, Dragan and Živković, Maja",
year = "2019",
abstract = "Background: Carotid plaque is a hallmark of advanced carotid atherosclerosis and there is evidence of phosphatase and actin regulator 1 (PHACTR1) involvement in the processes that lead to atherosclerosis. PHACTR1 intronic variants have been associated with coronary artery disease and carotid dissection. Up to date the PHACTR1 haplotypes were not investigated in association with carotid plaque presence (CPP). So, the aims of this study were to investigate possible association of PHACTR1 haplotypes inferred from the intronic variants rs9349379, rs2026458 and rs2876300 with CPP in patients with advanced carotid atherosclerosis and to analyze their possible effect on PHACTR1 relative mRNA expression in carotid plaque tissue specimens. Methods: The study group consisted of 501 patients with evidence of carotid plaque presence admitted for carotid endarterectomy and 310 healthy controls. PHACTR1 rs9349379, rs2026458, rs2876300 and relative mRNA expression were detected by TaqMan® technology. Results: We have found significant and independent association of haplotype ACA with the CPP, compared to the referent haplotype GTA (adjusted OR = 1.54 95% CI = 1.07–2.21, p = 0.02). The OR was adjusted for gender, age, BMI, hypertension and total cholesterol. The relative expression of PHACTR1 mRNA in carotid plaque tissue proved to be significantly higher in carriers of the ACG haplotype compared to the referent haplotype GTA (p = 0.03). Conclusion: Our results suggest that PHACTR1 haplotypes inferred from the variants rs9349379, rs2026458 and rs2876300 affect PHACTR1 mRNA and bear the risk for CPP in patients with advanced carotid atherosclerosis. Further replication and validation studies are inevitable. © 2019 Elsevier B.V.",
journal = "Gene",
title = "PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue",
volume = "710",
pages = "273-278",
doi = "10.1016/j.gene.2019.06.020"
}

Kuveljić, J., Đurić, T., Stanković, A., Končar, I., Alavantić, D.,& Živković, M.. (2019). PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue. in Gene, 710, 273-278.
https://doi.org/10.1016/j.gene.2019.06.020

Kuveljić J, Đurić T, Stanković A, Končar I, Alavantić D, Živković M. PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue. in Gene. 2019;710:273-278.
doi:10.1016/j.gene.2019.06.020 .

Kuveljić, Jovana, Đurić, Tamara, Stanković, Aleksandra, Končar, Igor, Alavantić, Dragan, Živković, Maja, "PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue" in Gene, 710 (2019):273-278,
https://doi.org/10.1016/j.gene.2019.06.020 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Milovanović, Branislav
AU  - Stanković, Aleksandra
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12682
AB  - Introduction: The autonomic nervous system (ANS), plays an essential role in the regulation of vascular wall contractility, heart rate and blood pressure (BP) variability. The symptoms of ANS dysfunction were strongly associated with vasovagal syncope (VVS). The renin-angiotensin system (RAS) is a powerful feedback system responsible for long-term control of cardiovascular hemodynamic values, heart rate and BP homeostasis. All components of the RAS have been detected in the brain as well as in the cardiovascular system, with central and peripheral actions of angiotensin II main effector molecule synthesized by angiotensin converting enzyme (ACE). Opposite influences of angiotensin II (AngII) receptor type 1 (AT1R) and angiotensin II receptor type 2 (ATR2) on sympathetic tone have been documented. Genetic studies have shown that individuals with VVS usually have a positive family history. Still, the genetic basis of VVS is unclear. The aim of the study was to investigate the association of the RAS gene variants, ACE I/D, AT1R A1166C and ATR2 -1332 A/G with the VVS in Serbian population. Methods: This case-control designed study included 215 VVS patients and 439 controls (C) matched by age (mean±SD, VVS: 38,25±13,52 years and C: 36,63±11,69 years). Genotyping was done by PCR-RFLP and allele specific PCR methods. Results: There were no significant differences in the genotype frequency distributions of ACE I/D and AT1R A1166C variants between cases and controls (p=0.4 and p=0.2, respectively). With regard to X-linked ATR2 -1332 A/G variant the frequency of A/- hemizygotes was significantly higher in VVS than in controls, in males (p=0.01). In females there were no differences in genotype frequency distributions between cases and controls (p=0.73). Conclusions: Our results suggest further investigation of AT2R hemodynamic effect on AngII in VVS males. To accurately elucidate given association, replication of the results in a larger sample is inevitable.
C3  - ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts
T1  - Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12682
ER  - 

@conference{
author = "Životić, Ivan and Kolić, Ivana and Đurić, Tamara and Živković, Maja and Milovanović, Branislav and Stanković, Aleksandra",
year = "2019",
abstract = "Introduction: The autonomic nervous system (ANS), plays an essential role in the regulation of vascular wall contractility, heart rate and blood pressure (BP) variability. The symptoms of ANS dysfunction were strongly associated with vasovagal syncope (VVS). The renin-angiotensin system (RAS) is a powerful feedback system responsible for long-term control of cardiovascular hemodynamic values, heart rate and BP homeostasis. All components of the RAS have been detected in the brain as well as in the cardiovascular system, with central and peripheral actions of angiotensin II main effector molecule synthesized by angiotensin converting enzyme (ACE). Opposite influences of angiotensin II (AngII) receptor type 1 (AT1R) and angiotensin II receptor type 2 (ATR2) on sympathetic tone have been documented. Genetic studies have shown that individuals with VVS usually have a positive family history. Still, the genetic basis of VVS is unclear. The aim of the study was to investigate the association of the RAS gene variants, ACE I/D, AT1R A1166C and ATR2 -1332 A/G with the VVS in Serbian population. Methods: This case-control designed study included 215 VVS patients and 439 controls (C) matched by age (mean±SD, VVS: 38,25±13,52 years and C: 36,63±11,69 years). Genotyping was done by PCR-RFLP and allele specific PCR methods. Results: There were no significant differences in the genotype frequency distributions of ACE I/D and AT1R A1166C variants between cases and controls (p=0.4 and p=0.2, respectively). With regard to X-linked ATR2 -1332 A/G variant the frequency of A/- hemizygotes was significantly higher in VVS than in controls, in males (p=0.01). In females there were no differences in genotype frequency distributions between cases and controls (p=0.73). Conclusions: Our results suggest further investigation of AT2R hemodynamic effect on AngII in VVS males. To accurately elucidate given association, replication of the results in a larger sample is inevitable.",
journal = "ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts",
title = "Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12682"
}

Životić, I., Kolić, I., Đurić, T., Živković, M., Milovanović, B.,& Stanković, A.. (2019). Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12682

Životić I, Kolić I, Đurić T, Živković M, Milovanović B, Stanković A. Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts. 2019;.
https://hdl.handle.net/21.15107/rcub_vinar_12682 .

Životić, Ivan, Kolić, Ivana, Đurić, Tamara, Živković, Maja, Milovanović, Branislav, Stanković, Aleksandra, "Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population" in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts (2019),
https://hdl.handle.net/21.15107/rcub_vinar_12682 .

TY  - CONF
AU  - Kolić, Ivana
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Alavantić, Dragan
AU  - Jovanović, Dušica
AU  - Milovanović, Branislav
AU  - Stanković, Aleksandra
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12683
AB  - Background: Disturbance in sympathovagal balance were strongly associated withoccurrence of syncope.The renin-angiotensin system (RAS) interacts with the autonomous nervous system (ANS) in the regulation of blood pressure and cardiovascular function. By now, several genetic variants in the RAS have been identified as factors in alteration of HRV parameters, haemodynamic values,heart rate, and BP.The aim of our study was to investigate the association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants and ANS function. Methods: This study included 215 syncope patients (age (mean±SD)=38.15±13.52 years, 79% females and 21% males) whose ANS function was evaluated by power spectral analysis of heart rate variability (HRV) before and during Tilt test (TT). Genotyping was done by PCR-RFLP and allele specific PCR methods. Statistical analysis was performed using STATISTICA data analysis software system (StatSoft, Inc. (2007). STATISTICA, version 8.0. www.statsoft.com). Results: There were no significant associations of ACE I/D or AGTR2 -1332A/G gene variants with the level of HRV parameters. We found that homozygotic carriers of both AGTR1 +1166A/C alleles have significantly increased LF component in supine position before TT, compared to heterozygote carriers (p=0,04, Mann-Whitney U test). During the TT there were no significant diferences in level of LF component with regard to AGTR1 +1166A/C genotypes. Conclusions: The present study suggest association of AGTR1 +1166A/C variant with LF component adrressing predisposition to syncopal event during TT. This association need to be confirmed in further genetic association studies on a larger sample.
C3  - ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts
T1  - Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12683
ER  - 

@conference{
author = "Kolić, Ivana and Životić, Ivan and Đurić, Tamara and Živković, Maja and Alavantić, Dragan and Jovanović, Dušica and Milovanović, Branislav and Stanković, Aleksandra",
year = "2019",
abstract = "Background: Disturbance in sympathovagal balance were strongly associated withoccurrence of syncope.The renin-angiotensin system (RAS) interacts with the autonomous nervous system (ANS) in the regulation of blood pressure and cardiovascular function. By now, several genetic variants in the RAS have been identified as factors in alteration of HRV parameters, haemodynamic values,heart rate, and BP.The aim of our study was to investigate the association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants and ANS function. Methods: This study included 215 syncope patients (age (mean±SD)=38.15±13.52 years, 79% females and 21% males) whose ANS function was evaluated by power spectral analysis of heart rate variability (HRV) before and during Tilt test (TT). Genotyping was done by PCR-RFLP and allele specific PCR methods. Statistical analysis was performed using STATISTICA data analysis software system (StatSoft, Inc. (2007). STATISTICA, version 8.0. www.statsoft.com). Results: There were no significant associations of ACE I/D or AGTR2 -1332A/G gene variants with the level of HRV parameters. We found that homozygotic carriers of both AGTR1 +1166A/C alleles have significantly increased LF component in supine position before TT, compared to heterozygote carriers (p=0,04, Mann-Whitney U test). During the TT there were no significant diferences in level of LF component with regard to AGTR1 +1166A/C genotypes. Conclusions: The present study suggest association of AGTR1 +1166A/C variant with LF component adrressing predisposition to syncopal event during TT. This association need to be confirmed in further genetic association studies on a larger sample.",
journal = "ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts",
title = "Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12683"
}

Kolić, I., Životić, I., Đurić, T., Živković, M., Alavantić, D., Jovanović, D., Milovanović, B.,& Stanković, A.. (2019). Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12683

Kolić I, Životić I, Đurić T, Živković M, Alavantić D, Jovanović D, Milovanović B, Stanković A. Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts. 2019;.
https://hdl.handle.net/21.15107/rcub_vinar_12683 .

Kolić, Ivana, Životić, Ivan, Đurić, Tamara, Živković, Maja, Alavantić, Dragan, Jovanović, Dušica, Milovanović, Branislav, Stanković, Aleksandra, "Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients" in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts (2019),
https://hdl.handle.net/21.15107/rcub_vinar_12683 .

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Milašinović, Dejan
AU  - Stanković, Goran
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8432
AB  - Background: Chromosomal region 9p21.3 is most robustly associated with coronary artery disease (CAD) in western European populations. However, heterogeneity in CAD phenotypes leads to uncertainty whether 9p21.3 is associated with stable and/or acute clinical presentations of CAD. 9p21.3 is rich in regulatory elements, but the underlying mechanisms of its actions in CAD remain unclear. We investigate the association of 9p21.3 two haplotype blocks lead variants (rs10757278 and rs518394) with first-ever non-fatal myocardial infarction (MI) in CAD patients and their association with CDKN2B mRNA expression in peripheral blood mononuclear cells 6 months after the event. Methods: We included CAD patients with sustained first MI (n = 523) and controls (n = 583). Gene expression was assessed in 72 patients 6 months after MI and 43 healthy controls. TaqMan® technology was used for the gene expression and genotyping analysis. Results: CDKN2B mRNA was significantly lower in MI patients compared with the controls (p = 0.002) and in patients carrying the rs10757278 G risk allele versus AA homozygotes (p = 0.012) 6 months after the event. While we confirmed the association of rs10757278 with CDKN2B expression in MI patients, we failed to find an association between the investigated variants and MI or disease burden. Conclusions: We suggest a dysregulation of gene expression in the 9p21.3 region six months after acute MI, which is affected by a genetic variant in patients. The rs10757278 rare allele is one factor that might lead to prolonged risk for proatherogenic complications. © 2019 The Canadian Society of Clinical Chemists
T2  - Clinical Biochemistry
T1  - CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI
VL  - 73
SP  - 70
EP  - 76
DO  - 10.1016/j.clinbiochem.2019.08.003
ER  - 

@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Milašinović, Dejan and Stanković, Goran and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Živković, Maja",
year = "2019",
abstract = "Background: Chromosomal region 9p21.3 is most robustly associated with coronary artery disease (CAD) in western European populations. However, heterogeneity in CAD phenotypes leads to uncertainty whether 9p21.3 is associated with stable and/or acute clinical presentations of CAD. 9p21.3 is rich in regulatory elements, but the underlying mechanisms of its actions in CAD remain unclear. We investigate the association of 9p21.3 two haplotype blocks lead variants (rs10757278 and rs518394) with first-ever non-fatal myocardial infarction (MI) in CAD patients and their association with CDKN2B mRNA expression in peripheral blood mononuclear cells 6 months after the event. Methods: We included CAD patients with sustained first MI (n = 523) and controls (n = 583). Gene expression was assessed in 72 patients 6 months after MI and 43 healthy controls. TaqMan® technology was used for the gene expression and genotyping analysis. Results: CDKN2B mRNA was significantly lower in MI patients compared with the controls (p = 0.002) and in patients carrying the rs10757278 G risk allele versus AA homozygotes (p = 0.012) 6 months after the event. While we confirmed the association of rs10757278 with CDKN2B expression in MI patients, we failed to find an association between the investigated variants and MI or disease burden. Conclusions: We suggest a dysregulation of gene expression in the 9p21.3 region six months after acute MI, which is affected by a genetic variant in patients. The rs10757278 rare allele is one factor that might lead to prolonged risk for proatherogenic complications. © 2019 The Canadian Society of Clinical Chemists",
journal = "Clinical Biochemistry",
title = "CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI",
volume = "73",
pages = "70-76",
doi = "10.1016/j.clinbiochem.2019.08.003"
}

Životić, I., Đurić, T., Stanković, A., Milašinović, D., Stanković, G., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Živković, M.. (2019). CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI. in Clinical Biochemistry, 73, 70-76.
https://doi.org/10.1016/j.clinbiochem.2019.08.003

Životić I, Đurić T, Stanković A, Milašinović D, Stanković G, Dekleva M, Marković-Nikolić N, Alavantić D, Živković M. CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI. in Clinical Biochemistry. 2019;73:70-76.
doi:10.1016/j.clinbiochem.2019.08.003 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Milašinović, Dejan, Stanković, Goran, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Živković, Maja, "CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI" in Clinical Biochemistry, 73 (2019):70-76,
https://doi.org/10.1016/j.clinbiochem.2019.08.003 . .

TY  - CONF
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Stanković, Aleksandra
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S002191501830707X
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7952
C3  - Atherosclerosis
T1  - The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype
VL  - 275
SP  - e135
DO  - 10.1016/j.atherosclerosis.2018.06.395
ER  - 

@conference{
author = "Kolaković, Ana and Živković, Maja and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra",
year = "2018",
journal = "Atherosclerosis",
title = "The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype",
volume = "275",
pages = "e135",
doi = "10.1016/j.atherosclerosis.2018.06.395"
}

Kolaković, A., Živković, M., Đurić, T., Končar, I.,& Stanković, A.. (2018). The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype. in Atherosclerosis, 275, e135.
https://doi.org/10.1016/j.atherosclerosis.2018.06.395

Kolaković A, Živković M, Đurić T, Končar I, Stanković A. The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype. in Atherosclerosis. 2018;275:e135.
doi:10.1016/j.atherosclerosis.2018.06.395 .

Kolaković, Ana, Živković, Maja, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, "The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype" in Atherosclerosis, 275 (2018):e135,
https://doi.org/10.1016/j.atherosclerosis.2018.06.395 . .

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Ivancevic, Ilija
AU  - Končar, Igor
AU  - Milašinović, Dejan
AU  - Stanković, Goran
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1843
AB  - The 9p21.3 region is rich in regulatory elements and the variants in this region had been robustly associated with carotid plaque (CP) and coronary artery disease (CAD). Recently, the HACD4 was detected as one of the six 9p21.3 differentially expressed genes associated with accelerated atherosclerosis and greater mean lesion area in the Athsq1 congenic mice. We aimed to investigate association of two potentially regulatory HACD4 variants (rs36212560 I/D, rs2275888 T/C) and their haplotypes with CP occurrence and the level of HACD4 and FOCAD mRNA in human CP tissue. Association study was replicated in CAD patients who suffered the first myocardial infarction. Study included 477 CP patients, 303 healthy controls and replication sample of 224 CAD males from the population of Serbia. Genotypes were determined by polymerase chain reaction (PCR) and real-time PCR using TaqMan (R) technology. The gene expression was detected with TaqMan (R) technology. We have found significant and independent association of DT haplotype with CP presence in men (adjusted OR = 1.64 CI = 1.12-2.42, p = 0.011). The result was replicated in CAD males (adjusted OR = 1.84 CI = 1.21-2.80, p = 0.004). We have found significant effect of the HACD4 rs2275888 on FOCAD mRNA level in human CP tissue. Correction for multiple testing was performed. Independent association of HACD4 haplotypes with atherosclerotic phenotypes connotes a further validation and replication in larger cohorts as well as functional studies to enlighten the potential mechanism of its action in pathophysiology of atherosclerosis.
T2  - Gene
T1  - The HACD4 haplotype as a risk factor for atherosclerosis in males
VL  - 641
SP  - 35
EP  - 40
DO  - 10.1016/j.gene.2017.10.030
ER  - 

@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Ivancevic, Ilija and Končar, Igor and Milašinović, Dejan and Stanković, Goran and Alavantić, Dragan and Živković, Maja",
year = "2018",
abstract = "The 9p21.3 region is rich in regulatory elements and the variants in this region had been robustly associated with carotid plaque (CP) and coronary artery disease (CAD). Recently, the HACD4 was detected as one of the six 9p21.3 differentially expressed genes associated with accelerated atherosclerosis and greater mean lesion area in the Athsq1 congenic mice. We aimed to investigate association of two potentially regulatory HACD4 variants (rs36212560 I/D, rs2275888 T/C) and their haplotypes with CP occurrence and the level of HACD4 and FOCAD mRNA in human CP tissue. Association study was replicated in CAD patients who suffered the first myocardial infarction. Study included 477 CP patients, 303 healthy controls and replication sample of 224 CAD males from the population of Serbia. Genotypes were determined by polymerase chain reaction (PCR) and real-time PCR using TaqMan (R) technology. The gene expression was detected with TaqMan (R) technology. We have found significant and independent association of DT haplotype with CP presence in men (adjusted OR = 1.64 CI = 1.12-2.42, p = 0.011). The result was replicated in CAD males (adjusted OR = 1.84 CI = 1.21-2.80, p = 0.004). We have found significant effect of the HACD4 rs2275888 on FOCAD mRNA level in human CP tissue. Correction for multiple testing was performed. Independent association of HACD4 haplotypes with atherosclerotic phenotypes connotes a further validation and replication in larger cohorts as well as functional studies to enlighten the potential mechanism of its action in pathophysiology of atherosclerosis.",
journal = "Gene",
title = "The HACD4 haplotype as a risk factor for atherosclerosis in males",
volume = "641",
pages = "35-40",
doi = "10.1016/j.gene.2017.10.030"
}

Životić, I., Đurić, T., Stanković, A., Ivancevic, I., Končar, I., Milašinović, D., Stanković, G., Alavantić, D.,& Živković, M.. (2018). The HACD4 haplotype as a risk factor for atherosclerosis in males. in Gene, 641, 35-40.
https://doi.org/10.1016/j.gene.2017.10.030

Životić I, Đurić T, Stanković A, Ivancevic I, Končar I, Milašinović D, Stanković G, Alavantić D, Živković M. The HACD4 haplotype as a risk factor for atherosclerosis in males. in Gene. 2018;641:35-40.
doi:10.1016/j.gene.2017.10.030 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Ivancevic, Ilija, Končar, Igor, Milašinović, Dejan, Stanković, Goran, Alavantić, Dragan, Živković, Maja, "The HACD4 haplotype as a risk factor for atherosclerosis in males" in Gene, 641 (2018):35-40,
https://doi.org/10.1016/j.gene.2017.10.030 . .

TY  - JOUR
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Kostić, Mirjana M.
AU  - Krstić, Zoran
AU  - Đurić, Tamara
AU  - Licastro, Danilo
AU  - Meroni, Germana
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0024320518305940
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7891
AB  - Aims: (1) to identify the most dysregulated genes in ureter tissue affected by congenital anomalies of the kidney and urinary tract (CAKUT) and to extract the biological meaning of these markers; (2) to describe the key molecular networks in CAKUT and to provide expression validation of the genes selected from these networks. Main methods: Transcriptome data was obtained from ureter samples of CAKUT patients and controls by Illumina iScan microarray. Identification of differentially expressed genes was coupled with subsequent bioinformatics analyses. Expression of candidate genes was validated by qRT-PCR. Key findings: Analysis of the transcriptome led to the identification of 78 commonly dysregulated genes in CAKUT tissue compared to controls. Integrative bioinformatic analyses of differentially expressed genes identified 7 major networks. The targets for qRT-PCR validation were selected as members of the major molecular networks in CAKUT, which had both, the significant high fold change and biological relevance for CAKUT. By qRT-PCR the substantial increase of LCN2, PROM1, SOSTDC1, and decrease of INA, RASD1 and TAC3 mRNA levels was confirmed. Significance: Since CAKUT is a leading cause of end-stage renal disease in children, the search for molecular targets for postnatal therapy is of particular interest. Data described in this study represents the gene expression profile and significant molecular networks specific to human ureter affected by CAKUT. The discovery of impaired molecular factors and processes is the step towards the uncovering of the key mechanisms that reflect CAKUT postnatally and could lead to the affected tissue deterioration and end organ damage. © 2018 Elsevier Inc.
T2  - Life Sciences
T1  - Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT
VL  - 212
SP  - 1
EP  - 8
DO  - 10.1016/j.lfs.2018.09.042
ER  - 

@article{
author = "Jovanović, Ivan G. and Živković, Maja and Kostić, Mirjana M. and Krstić, Zoran and Đurić, Tamara and Licastro, Danilo and Meroni, Germana and Alavantić, Dragan and Stanković, Aleksandra",
year = "2018",
abstract = "Aims: (1) to identify the most dysregulated genes in ureter tissue affected by congenital anomalies of the kidney and urinary tract (CAKUT) and to extract the biological meaning of these markers; (2) to describe the key molecular networks in CAKUT and to provide expression validation of the genes selected from these networks. Main methods: Transcriptome data was obtained from ureter samples of CAKUT patients and controls by Illumina iScan microarray. Identification of differentially expressed genes was coupled with subsequent bioinformatics analyses. Expression of candidate genes was validated by qRT-PCR. Key findings: Analysis of the transcriptome led to the identification of 78 commonly dysregulated genes in CAKUT tissue compared to controls. Integrative bioinformatic analyses of differentially expressed genes identified 7 major networks. The targets for qRT-PCR validation were selected as members of the major molecular networks in CAKUT, which had both, the significant high fold change and biological relevance for CAKUT. By qRT-PCR the substantial increase of LCN2, PROM1, SOSTDC1, and decrease of INA, RASD1 and TAC3 mRNA levels was confirmed. Significance: Since CAKUT is a leading cause of end-stage renal disease in children, the search for molecular targets for postnatal therapy is of particular interest. Data described in this study represents the gene expression profile and significant molecular networks specific to human ureter affected by CAKUT. The discovery of impaired molecular factors and processes is the step towards the uncovering of the key mechanisms that reflect CAKUT postnatally and could lead to the affected tissue deterioration and end organ damage. © 2018 Elsevier Inc.",
journal = "Life Sciences",
title = "Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT",
volume = "212",
pages = "1-8",
doi = "10.1016/j.lfs.2018.09.042"
}

Jovanović, I. G., Živković, M., Kostić, M. M., Krstić, Z., Đurić, T., Licastro, D., Meroni, G., Alavantić, D.,& Stanković, A.. (2018). Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT. in Life Sciences, 212, 1-8.
https://doi.org/10.1016/j.lfs.2018.09.042

Jovanović IG, Živković M, Kostić MM, Krstić Z, Đurić T, Licastro D, Meroni G, Alavantić D, Stanković A. Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT. in Life Sciences. 2018;212:1-8.
doi:10.1016/j.lfs.2018.09.042 .

Jovanović, Ivan G., Živković, Maja, Kostić, Mirjana M., Krstić, Zoran, Đurić, Tamara, Licastro, Danilo, Meroni, Germana, Alavantić, Dragan, Stanković, Aleksandra, "Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT" in Life Sciences, 212 (2018):1-8,
https://doi.org/10.1016/j.lfs.2018.09.042 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Stanković, Goran
AU  - Milašinović, Dejan
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018309699
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7953
AB  - Aim: Myocardial infarction (MI) incidence is still a major burden in the
terms of premature death and disability world-wide. Genetic studies up to
date explained a limited portion of disease inheritance. Recently, the DT
haplotype of variants rs36212560 I/D (insertion/deletion) and 2275888 T/C
in HACD gene (9p21.3) have been significantly associated with the risk of
carotid plaque occurrence among males.
Methods: We aimed to validate these results by investigating 518 MI patients (366 males) and 303 (167 males) healthy controls from Serbia. Also, he HACD4 gene expression analysis has been conducted in the peripheral
blood mononuclear cells of 72 MI patients, 6 months after the MI. Results
were obtained using ABI-Prism 7500 RT-PCR for rs2275888 allelic
discrimination and HACD4 mRNA relative quantitation. PCR and acrylamide gel electrophoresis were used to distinguish 5 base pairs
rs36212560 insertion deletion polymorphisms.
Results: Haplotype analysis (using Thesias software) showed that DT
haplotype carriers had significantly higher risk for MI (OR ¼ 1.42, CI 1.08-
1.85, p¼0.01) compared with most frequent IT haplotype. In gender
separated groups association remained significant only among males (OR
¼ 1.76, 1.266-2.46, p¼0.0008). Results were adjusted for MI traditional risk
factors (Age, BMI, HDLC, LDLC, Tg, hypertension and smoking). Investigated
genetic variants were not associated with HACD4 expression. Significant
correlation was found between HACD4 mRNA level and age (r¼0.36,
p¼0.001).
Conclusions: We have shown that rs36212560 and rs2275888 DT haplotype from HACD4 gene is significantly and independently associated with
the MI occurrence in males. Additional studies are needed to confirm these
results.
C3  - Atherosclerosis
T1  - HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia
VL  - 275
SP  - e210
EP  - e211
DO  - 10.1016/j.atherosclerosis.2018.06.657
ER  - 

@conference{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Stanković, Goran and Milašinović, Dejan and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Živković, Maja",
year = "2018",
abstract = "Aim: Myocardial infarction (MI) incidence is still a major burden in the
terms of premature death and disability world-wide. Genetic studies up to
date explained a limited portion of disease inheritance. Recently, the DT
haplotype of variants rs36212560 I/D (insertion/deletion) and 2275888 T/C
in HACD gene (9p21.3) have been significantly associated with the risk of
carotid plaque occurrence among males.
Methods: We aimed to validate these results by investigating 518 MI patients (366 males) and 303 (167 males) healthy controls from Serbia. Also, he HACD4 gene expression analysis has been conducted in the peripheral
blood mononuclear cells of 72 MI patients, 6 months after the MI. Results
were obtained using ABI-Prism 7500 RT-PCR for rs2275888 allelic
discrimination and HACD4 mRNA relative quantitation. PCR and acrylamide gel electrophoresis were used to distinguish 5 base pairs
rs36212560 insertion deletion polymorphisms.
Results: Haplotype analysis (using Thesias software) showed that DT
haplotype carriers had significantly higher risk for MI (OR ¼ 1.42, CI 1.08-
1.85, p¼0.01) compared with most frequent IT haplotype. In gender
separated groups association remained significant only among males (OR
¼ 1.76, 1.266-2.46, p¼0.0008). Results were adjusted for MI traditional risk
factors (Age, BMI, HDLC, LDLC, Tg, hypertension and smoking). Investigated
genetic variants were not associated with HACD4 expression. Significant
correlation was found between HACD4 mRNA level and age (r¼0.36,
p¼0.001).
Conclusions: We have shown that rs36212560 and rs2275888 DT haplotype from HACD4 gene is significantly and independently associated with
the MI occurrence in males. Additional studies are needed to confirm these
results.",
journal = "Atherosclerosis",
title = "HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia",
volume = "275",
pages = "e210-e211",
doi = "10.1016/j.atherosclerosis.2018.06.657"
}

Životić, I., Đurić, T., Stanković, A., Stanković, G., Milašinović, D., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Živković, M.. (2018). HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia. in Atherosclerosis, 275, e210-e211.
https://doi.org/10.1016/j.atherosclerosis.2018.06.657

Životić I, Đurić T, Stanković A, Stanković G, Milašinović D, Dekleva M, Marković-Nikolić N, Alavantić D, Živković M. HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia. in Atherosclerosis. 2018;275:e210-e211.
doi:10.1016/j.atherosclerosis.2018.06.657 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Stanković, Goran, Milašinović, Dejan, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Živković, Maja, "HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia" in Atherosclerosis, 275 (2018):e210-e211,
https://doi.org/10.1016/j.atherosclerosis.2018.06.657 . .

TY  - JOUR
AU  - Đorđević, Ana D.
AU  - Dekleva, Milica
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Đurić, Tamara
PY  - 2018
UR  - http://link.springer.com/10.1007/s11033-018-4384-4
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7958
AB  - Post-infarct left ventricular remodeling (LVR) process increases the risk of heart failure (HF). Circulating galectin-3 has been associated with fibrosis, inflammation and cardiac dysfunction during the remodeling process after myocardial infarction (MI). The aims of this prospective case study were to investigate the association of potentially functional variants in the vicinity of LGALS-3 locus, rs2274273 and rs17128183 with maladaptive LVR and whether these variants could affect LGALS-3 mRNA expression in peripheral blood mononuclear cells of patients 6 months after the first MI. This study encompassed 167 patients with acute MI that were followed up for 6 months. Evidence of LVR was obtained by repeated 2D Doppler echocardiography. Rs2274273, rs17128183 and LGALS-3 mRNA expression were detected by TaqMan® technology. Rs2274273 and rs17128183 rare allele bearing genotypes, according to the dominant model (CT+TT vs. CC and AG+GG vs. AA, respectively), were significantly and independently associated with maladaptive LVR (adjusted OR = 3.02, P = 0.016; adjusted OR = 3.14, P = 0.019, respectively) and higher LGALS-3 mRNA expression (fold induction 1.203, P = 0.03 and 1.214, P = 0.03, respectively). Our exploratory results suggest that rs2274273 and rs17128183 variants affect LGALS-3 mRNA and bear the risk for maladaptive LVR post-MI remodeling. Further replication and validation in a larger group of patients is inevitable. © 2018, Springer Nature B.V.
T2  - Molecular Biology Reports
T1  - Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study
VL  - 45
IS  - 6
SP  - 2227
EP  - 2236
DO  - 10.1007/s11033-018-4384-4
ER  - 

@article{
author = "Đorđević, Ana D. and Dekleva, Milica and Živković, Maja and Stanković, Aleksandra and Marković-Nikolić, Nataša and Alavantić, Dragan and Đurić, Tamara",
year = "2018",
abstract = "Post-infarct left ventricular remodeling (LVR) process increases the risk of heart failure (HF). Circulating galectin-3 has been associated with fibrosis, inflammation and cardiac dysfunction during the remodeling process after myocardial infarction (MI). The aims of this prospective case study were to investigate the association of potentially functional variants in the vicinity of LGALS-3 locus, rs2274273 and rs17128183 with maladaptive LVR and whether these variants could affect LGALS-3 mRNA expression in peripheral blood mononuclear cells of patients 6 months after the first MI. This study encompassed 167 patients with acute MI that were followed up for 6 months. Evidence of LVR was obtained by repeated 2D Doppler echocardiography. Rs2274273, rs17128183 and LGALS-3 mRNA expression were detected by TaqMan® technology. Rs2274273 and rs17128183 rare allele bearing genotypes, according to the dominant model (CT+TT vs. CC and AG+GG vs. AA, respectively), were significantly and independently associated with maladaptive LVR (adjusted OR = 3.02, P = 0.016; adjusted OR = 3.14, P = 0.019, respectively) and higher LGALS-3 mRNA expression (fold induction 1.203, P = 0.03 and 1.214, P = 0.03, respectively). Our exploratory results suggest that rs2274273 and rs17128183 variants affect LGALS-3 mRNA and bear the risk for maladaptive LVR post-MI remodeling. Further replication and validation in a larger group of patients is inevitable. © 2018, Springer Nature B.V.",
journal = "Molecular Biology Reports",
title = "Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study",
volume = "45",
number = "6",
pages = "2227-2236",
doi = "10.1007/s11033-018-4384-4"
}

Đorđević, A. D., Dekleva, M., Živković, M., Stanković, A., Marković-Nikolić, N., Alavantić, D.,& Đurić, T.. (2018). Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study. in Molecular Biology Reports, 45(6), 2227-2236.
https://doi.org/10.1007/s11033-018-4384-4

Đorđević AD, Dekleva M, Živković M, Stanković A, Marković-Nikolić N, Alavantić D, Đurić T. Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study. in Molecular Biology Reports. 2018;45(6):2227-2236.
doi:10.1007/s11033-018-4384-4 .

Đorđević, Ana D., Dekleva, Milica, Živković, Maja, Stanković, Aleksandra, Marković-Nikolić, Nataša, Alavantić, Dragan, Đurić, Tamara, "Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study" in Molecular Biology Reports, 45, no. 6 (2018):2227-2236,
https://doi.org/10.1007/s11033-018-4384-4 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Jovanović, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Dekleva, Milica
AU  - Marković Nikolić, Nevena
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12816
AB  - Introduction: Recently, rs2275888 eQTL in PTPLAD2 gene has been associated with expression of several loci, during inflammatory stimulation in monocytes. Myocardial infarction (MI) triggers an intense inflammatory response that is essential for cardiac repair. We aimed to perform data scouting in appropriate rs2275888 genotype model to identify differentially expressed genes (DEGs), their biological meaning, and key miRs potentially associated with rs2275888 eQTL in peripheral blood mononuclear leucocytes (PBML) of MI patients 6 months after first MI. Methods: Transcriptome data was obtained from PBMLs of 21 patients, who suffered ischemic MI, by employing Illumina iScan microarray technology. Genotyping for rs2275888 was conducted with real-time PCR, using TaqMan® assay. Preprocessing and identification of DEGs was done using limma package of R/Bioconductor software. The online tool DAVID v6.8 was employed for functional enrichment analysis. Most important miRs were selected using NetworkAnalyst web tool, based on the degree centrality value. Results: Transcriptome analysis in recessive model TT+TC (n=19) vs. CC (n=2) identified 68 DEGs. Top significant biological processes involving DEGs cover vascular physiology, cell growth and signaling. Pathway analysis associated DEGs with adherens junction, Rap1 and Ras signaling. Network analysis identified hsa-miR335-5p, -26b-5p, -93-5p, -16-5p, -124-3p, -20b-5p, -17-5p and -218-5p as miRs with top centrality degree in our DEGs list. Conclusion: Seven of eight detected miRs have already been described in MI pathology or suggested as potential biomarkers for MI. Our result suggest the importance of integration of eQTLs, biological processes and pathway analysis coupled with miR activity for further research in MI pathology.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach
SP  - 176
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12816
ER  - 

@conference{
author = "Životić, Ivan and Jovanović, Ivan and Đurić, Tamara and Stanković, Aleksandra and Dekleva, Milica and Marković Nikolić, Nevena and Alavantić, Dragan and Živković, Maja",
year = "2017",
abstract = "Introduction: Recently, rs2275888 eQTL in PTPLAD2 gene has been associated with expression of several loci, during inflammatory stimulation in monocytes. Myocardial infarction (MI) triggers an intense inflammatory response that is essential for cardiac repair. We aimed to perform data scouting in appropriate rs2275888 genotype model to identify differentially expressed genes (DEGs), their biological meaning, and key miRs potentially associated with rs2275888 eQTL in peripheral blood mononuclear leucocytes (PBML) of MI patients 6 months after first MI. Methods: Transcriptome data was obtained from PBMLs of 21 patients, who suffered ischemic MI, by employing Illumina iScan microarray technology. Genotyping for rs2275888 was conducted with real-time PCR, using TaqMan® assay. Preprocessing and identification of DEGs was done using limma package of R/Bioconductor software. The online tool DAVID v6.8 was employed for functional enrichment analysis. Most important miRs were selected using NetworkAnalyst web tool, based on the degree centrality value. Results: Transcriptome analysis in recessive model TT+TC (n=19) vs. CC (n=2) identified 68 DEGs. Top significant biological processes involving DEGs cover vascular physiology, cell growth and signaling. Pathway analysis associated DEGs with adherens junction, Rap1 and Ras signaling. Network analysis identified hsa-miR335-5p, -26b-5p, -93-5p, -16-5p, -124-3p, -20b-5p, -17-5p and -218-5p as miRs with top centrality degree in our DEGs list. Conclusion: Seven of eight detected miRs have already been described in MI pathology or suggested as potential biomarkers for MI. Our result suggest the importance of integration of eQTLs, biological processes and pathway analysis coupled with miR activity for further research in MI pathology.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach",
pages = "176-176",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12816"
}

Životić, I., Jovanović, I., Đurić, T., Stanković, A., Dekleva, M., Marković Nikolić, N., Alavantić, D.,& Živković, M.. (2017). Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 176-176.
https://hdl.handle.net/21.15107/rcub_vinar_12816

Životić I, Jovanović I, Đurić T, Stanković A, Dekleva M, Marković Nikolić N, Alavantić D, Živković M. Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:176-176.
https://hdl.handle.net/21.15107/rcub_vinar_12816 .

Životić, Ivan, Jovanović, Ivan, Đurić, Tamara, Stanković, Aleksandra, Dekleva, Milica, Marković Nikolić, Nevena, Alavantić, Dragan, Živković, Maja, "Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):176-176,
https://hdl.handle.net/21.15107/rcub_vinar_12816 .

TY  - CONF
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7177
C3  - Atherosclerosis
T1  - Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results
VL  - 263
SP  - E180
EP  - E180
DO  - 10.1016/j.atherosclerosis.2017.06.578
ER  - 

@conference{
author = "Đorđević, Ana and Živković, Maja and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Stanković, Aleksandra and Đurić, Tamara",
year = "2017",
journal = "Atherosclerosis",
title = "Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results",
volume = "263",
pages = "E180-E180",
doi = "10.1016/j.atherosclerosis.2017.06.578"
}

Đorđević, A., Živković, M., Dekleva, M., Marković-Nikolić, N., Alavantić, D., Stanković, A.,& Đurić, T.. (2017). Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results. in Atherosclerosis, 263, E180-E180.
https://doi.org/10.1016/j.atherosclerosis.2017.06.578

Đorđević A, Živković M, Dekleva M, Marković-Nikolić N, Alavantić D, Stanković A, Đurić T. Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results. in Atherosclerosis. 2017;263:E180-E180.
doi:10.1016/j.atherosclerosis.2017.06.578 .

Đorđević, Ana, Živković, Maja, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Stanković, Aleksandra, Đurić, Tamara, "Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results" in Atherosclerosis, 263 (2017):E180-E180,
https://doi.org/10.1016/j.atherosclerosis.2017.06.578 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Đorđević, Ana
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nevena
AU  - Alavantić, Dragan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7178
AB  - Aim: Myocardial infarction (MI) is the clinical complication predominately
caused by coronary plaque buildup and rupture during the process of
atherosclerosis. In the 9p21 locus two important risk haplotype blocks
have been identified. The one that carries the risk for MI with the lead
variant rs10757278 and another characterized to influence progression of
the MI, with the lead variant rs518394. We have investigated association of
the two genetic variants with the ST-elevated MI in the gender specific
manner. We have also tested variants effect on p15 mRNA level as one of
the possible mechanisms of the variants effect.Methods: The study group included 147 patients (72 females) with angiographically assessed MI, and 240 healthy controls (90 females). DNA
and RNA (n¼28) where isolated from peripheral blood mono nuclear
leukocytes. Genotypes for rs10757278 A/G and rs518394 C/G, and relative
mRNA level for p15 were determined using commercial TaqMan® assays
on 7500 ABI Real-Time PCR.
Results: We have found significant association of rs10757278 GG with STelevated MI occurrence, with OR of 2.2 (CI¼1.07-4.5, p¼0.03) in females.
P15 mRNA was significantly down-regulated in G allele carriers (AG+GG vs
AA) by a mean factor of 0.449 (S.E. range is 0.188-1.059), p¼0.019 in the
whole group. The genetic variant rs518394 was not significantly associated
either with MI or p15 mRNA level.
Conclusions: Genotype GG of rs10757278 is significantly associated with
MI occurrence in females in Serbian population. On
C3  - Atherosclerosis
T1  - RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia
VL  - 263
SP  - e188
EP  - e188
DO  - 10.1016/j.atherosclerosis.2017.06.603
ER  - 

@conference{
author = "Životić, Ivan and Živković, Maja and Đurić, Tamara and Stanković, Aleksandra and Đorđević, Ana and Dekleva, Milica and Marković-Nikolić, Nevena and Alavantić, Dragan",
year = "2017",
abstract = "Aim: Myocardial infarction (MI) is the clinical complication predominately
caused by coronary plaque buildup and rupture during the process of
atherosclerosis. In the 9p21 locus two important risk haplotype blocks
have been identified. The one that carries the risk for MI with the lead
variant rs10757278 and another characterized to influence progression of
the MI, with the lead variant rs518394. We have investigated association of
the two genetic variants with the ST-elevated MI in the gender specific
manner. We have also tested variants effect on p15 mRNA level as one of
the possible mechanisms of the variants effect.Methods: The study group included 147 patients (72 females) with angiographically assessed MI, and 240 healthy controls (90 females). DNA
and RNA (n¼28) where isolated from peripheral blood mono nuclear
leukocytes. Genotypes for rs10757278 A/G and rs518394 C/G, and relative
mRNA level for p15 were determined using commercial TaqMan® assays
on 7500 ABI Real-Time PCR.
Results: We have found significant association of rs10757278 GG with STelevated MI occurrence, with OR of 2.2 (CI¼1.07-4.5, p¼0.03) in females.
P15 mRNA was significantly down-regulated in G allele carriers (AG+GG vs
AA) by a mean factor of 0.449 (S.E. range is 0.188-1.059), p¼0.019 in the
whole group. The genetic variant rs518394 was not significantly associated
either with MI or p15 mRNA level.
Conclusions: Genotype GG of rs10757278 is significantly associated with
MI occurrence in females in Serbian population. On",
journal = "Atherosclerosis",
title = "RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia",
volume = "263",
pages = "e188-e188",
doi = "10.1016/j.atherosclerosis.2017.06.603"
}

Životić, I., Živković, M., Đurić, T., Stanković, A., Đorđević, A., Dekleva, M., Marković-Nikolić, N.,& Alavantić, D.. (2017). RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia. in Atherosclerosis, 263, e188-e188.
https://doi.org/10.1016/j.atherosclerosis.2017.06.603

Životić I, Živković M, Đurić T, Stanković A, Đorđević A, Dekleva M, Marković-Nikolić N, Alavantić D. RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia. in Atherosclerosis. 2017;263:e188-e188.
doi:10.1016/j.atherosclerosis.2017.06.603 .

Životić, Ivan, Živković, Maja, Đurić, Tamara, Stanković, Aleksandra, Đorđević, Ana, Dekleva, Milica, Marković-Nikolić, Nevena, Alavantić, Dragan, "RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia" in Atherosclerosis, 263 (2017):e188-e188,
https://doi.org/10.1016/j.atherosclerosis.2017.06.603 . .