TY  - JOUR
AU  - Đurić, Dragan M.
AU  - Drndarevic, V
AU  - Vujic, D
PY  - 1999
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2290
AB  - A freezing device for cryopreservation of blood manonuclear cells has been developed. The device is microcontroller operated, allowing cell freezing by a fully automatic, unattended process. To ensure optimum preservation, the temperature in the cell suspension uniformly decreases from room temperature to -100 degrees C and then the samples are transferred to long-term storage. The performance of the device has been tested using both physiological solution and a sample of cell suspension. The control of temperature variation of cell suspension in the entire temperature range has been realised with an accuracy better than +/-0.1%. The viability of cells recovered from the frozen samples was 95%. The nitrogen consumption for one cycle of cryopreservation was 1.51. In addition to the fully automatic mode, the manual and semi-automatic modes are available for research purposes. The device has been designed using low cost and widely used electronic components and materials, it is compact and simple to operate.
T2  - Bio-Medical Materials and Engineering
T1  - Microprocessor-controlled freezing device for cryopreservation of cell samples
VL  - 9
IS  - 3
SP  - 171
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2290
ER  - 

@article{
author = "Đurić, Dragan M. and Drndarevic, V and Vujic, D",
year = "1999",
abstract = "A freezing device for cryopreservation of blood manonuclear cells has been developed. The device is microcontroller operated, allowing cell freezing by a fully automatic, unattended process. To ensure optimum preservation, the temperature in the cell suspension uniformly decreases from room temperature to -100 degrees C and then the samples are transferred to long-term storage. The performance of the device has been tested using both physiological solution and a sample of cell suspension. The control of temperature variation of cell suspension in the entire temperature range has been realised with an accuracy better than +/-0.1%. The viability of cells recovered from the frozen samples was 95%. The nitrogen consumption for one cycle of cryopreservation was 1.51. In addition to the fully automatic mode, the manual and semi-automatic modes are available for research purposes. The device has been designed using low cost and widely used electronic components and materials, it is compact and simple to operate.",
journal = "Bio-Medical Materials and Engineering",
title = "Microprocessor-controlled freezing device for cryopreservation of cell samples",
volume = "9",
number = "3",
pages = "171-178",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2290"
}

Đurić, D. M., Drndarevic, V.,& Vujic, D.. (1999). Microprocessor-controlled freezing device for cryopreservation of cell samples. in Bio-Medical Materials and Engineering, 9(3), 171-178.
https://hdl.handle.net/21.15107/rcub_vinar_2290

Đurić DM, Drndarevic V, Vujic D. Microprocessor-controlled freezing device for cryopreservation of cell samples. in Bio-Medical Materials and Engineering. 1999;9(3):171-178.
https://hdl.handle.net/21.15107/rcub_vinar_2290 .

Đurić, Dragan M., Drndarevic, V, Vujic, D, "Microprocessor-controlled freezing device for cryopreservation of cell samples" in Bio-Medical Materials and Engineering, 9, no. 3 (1999):171-178,
https://hdl.handle.net/21.15107/rcub_vinar_2290 .