TY  - JOUR
AU  - Knežević, Dragan
AU  - Bošnjaković, Dušan
AU  - Dražić, Slavica
AU  - Nedić, Sreten
AU  - Vujanac, Ivan
AU  - Valčić, Olivera
AU  - Pantelić, Marija
AU  - Stojiljković, Mojca
AU  - Sladojević, Željko
AU  - Kirovski, Danijela
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13027
AB  - The study aimed to determine the concentration of energy-related hormones in cow’s milk and to consider them from a public health perspective. Fourteen Holstein cows were subjected to milk and blood sampling on the following days in lactation (DIL): 10, 30, 60, 90, 150, 180, 210, 250 and 280 to determine milk hormones, fat and protein content and blood biochemical parameters. For the same purpose, bulk-tank milk was sampled and samples of retail m ilk with 1.5% (CM1.5) and 3.2% (CM3.2) fat was purchased. Milk insulin-like growth factor-1 (IGF-1) values were signifi cantly lower at 90, 150, 180, 210 and 250 and signifi cantly higher at 10, 30 and 60 DIL than lactation average (LA). Milk insulin concentrations were signifi cantly lower at 30, 60 and 90 and higher at 210, 250 and 280 DIL than LA. Free thyroxine (fT4) level in the milk was higher at 250 DIL, while milk free triiodothyronine (fT3) concentrations were lower at 30, 60, 90 and 280 DIL, and signifi cantly higher at 10 and 180 DIL than respective LA. Milk cortisol levels were lower at 60 and 280 DIL than LA. All measured milk hormones were signifi cantly lower in CM1.5 compared to CM3.2, bulk-tank milk and LA. An exception was the LA of IGF-1, which was signifi cantly lower than the IGF1 content in CM1.5. Blood biochemical parameters fl uctuated evenly during lactation and were within the reference range. Hormone concentrations in cow’s milk fl uctuate during lactation, giving milk an important role in the context of public health.
AB  - Istraživanje je imalo za cilj određivanje koncentracije hormona u mleku krava u različitim fazama laktacije, mleku iz laktofriza i maloprodajnom mleku. Uzorci mleka i krvi prikupljeni su 10., 30., 60., 90., 150., 180., 210., 250. i 280 dana laktacije od četrnaest krava holštajn rase radi određivanja koncentracije hormona, masti i proteina u mleku i biohemijskih parametara krvi. U iste svrhe uzorkovano je mleko iz laktofriza i nabavljeno je komercijalno mleko sa 1,5% (CM1,5) i 3,2% (CM3,2) mlečne masti. Koncentracije insulinu-sličnog faktora rasta-1 (IGF-1) u mleku bile su značajno niže 90., 150., 180., 210. i 250. dana i značajno više 10., 30. i 60. dana laktacije poredeći sa laktacionim prosekom (LP). Insulin je imao značajno niže koncentracije u mleku 30., 60. i 90. dana i značajno više koncentracije 210., 250. i 280. dana laktacije poredeći sa LP. Koncentracija slobodnog tiroksina (fT4) je bila viša 250. dana, dok je nivo slobodnog trijodotironina (fT3) bio značajno niži 30., 60., 90. i 280. dana i značajno viši 10. i 180. dana u poređenju sa odgovarajućim LP. Nivo kortizola u mleku je bio niži 60. i 280. dana laktacije poredeći sa LP. Svi hormoni su imali značajno niže koncentracije u CM1,5 nego u CM3,2, mleku iz laktofriza i LP. Izuzetak je LP za IGF-1, koji je bio značajno niži nego nivo IGF-1 u CM1,5. Biohemijski parametri krvi su blago fl uktuirali tokom laktacije, ali su ostali u referentnom opsegu. Koncentracije hormona u kravljem mleku fl uktuiraju tokom laktacije, što daje značaj mleku u kontekstu javnog zdravlja.
T2  - Acta Veterinaria
T1  - Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers
T1  - Hormoni u sirovom i maloprodajnom mleku krava i potencijalni rizik po zdravlje potrošača
VL  - 74
IS  - 1
SP  - 1
EP  - 16
DO  - 10.2478/acve-2024-0001
ER  - 

@article{
author = "Knežević, Dragan and Bošnjaković, Dušan and Dražić, Slavica and Nedić, Sreten and Vujanac, Ivan and Valčić, Olivera and Pantelić, Marija and Stojiljković, Mojca and Sladojević, Željko and Kirovski, Danijela",
year = "2024",
abstract = "The study aimed to determine the concentration of energy-related hormones in cow’s milk and to consider them from a public health perspective. Fourteen Holstein cows were subjected to milk and blood sampling on the following days in lactation (DIL): 10, 30, 60, 90, 150, 180, 210, 250 and 280 to determine milk hormones, fat and protein content and blood biochemical parameters. For the same purpose, bulk-tank milk was sampled and samples of retail m ilk with 1.5% (CM1.5) and 3.2% (CM3.2) fat was purchased. Milk insulin-like growth factor-1 (IGF-1) values were signifi cantly lower at 90, 150, 180, 210 and 250 and signifi cantly higher at 10, 30 and 60 DIL than lactation average (LA). Milk insulin concentrations were signifi cantly lower at 30, 60 and 90 and higher at 210, 250 and 280 DIL than LA. Free thyroxine (fT4) level in the milk was higher at 250 DIL, while milk free triiodothyronine (fT3) concentrations were lower at 30, 60, 90 and 280 DIL, and signifi cantly higher at 10 and 180 DIL than respective LA. Milk cortisol levels were lower at 60 and 280 DIL than LA. All measured milk hormones were signifi cantly lower in CM1.5 compared to CM3.2, bulk-tank milk and LA. An exception was the LA of IGF-1, which was signifi cantly lower than the IGF1 content in CM1.5. Blood biochemical parameters fl uctuated evenly during lactation and were within the reference range. Hormone concentrations in cow’s milk fl uctuate during lactation, giving milk an important role in the context of public health., Istraživanje je imalo za cilj određivanje koncentracije hormona u mleku krava u različitim fazama laktacije, mleku iz laktofriza i maloprodajnom mleku. Uzorci mleka i krvi prikupljeni su 10., 30., 60., 90., 150., 180., 210., 250. i 280 dana laktacije od četrnaest krava holštajn rase radi određivanja koncentracije hormona, masti i proteina u mleku i biohemijskih parametara krvi. U iste svrhe uzorkovano je mleko iz laktofriza i nabavljeno je komercijalno mleko sa 1,5% (CM1,5) i 3,2% (CM3,2) mlečne masti. Koncentracije insulinu-sličnog faktora rasta-1 (IGF-1) u mleku bile su značajno niže 90., 150., 180., 210. i 250. dana i značajno više 10., 30. i 60. dana laktacije poredeći sa laktacionim prosekom (LP). Insulin je imao značajno niže koncentracije u mleku 30., 60. i 90. dana i značajno više koncentracije 210., 250. i 280. dana laktacije poredeći sa LP. Koncentracija slobodnog tiroksina (fT4) je bila viša 250. dana, dok je nivo slobodnog trijodotironina (fT3) bio značajno niži 30., 60., 90. i 280. dana i značajno viši 10. i 180. dana u poređenju sa odgovarajućim LP. Nivo kortizola u mleku je bio niži 60. i 280. dana laktacije poredeći sa LP. Svi hormoni su imali značajno niže koncentracije u CM1,5 nego u CM3,2, mleku iz laktofriza i LP. Izuzetak je LP za IGF-1, koji je bio značajno niži nego nivo IGF-1 u CM1,5. Biohemijski parametri krvi su blago fl uktuirali tokom laktacije, ali su ostali u referentnom opsegu. Koncentracije hormona u kravljem mleku fl uktuiraju tokom laktacije, što daje značaj mleku u kontekstu javnog zdravlja.",
journal = "Acta Veterinaria",
title = "Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers, Hormoni u sirovom i maloprodajnom mleku krava i potencijalni rizik po zdravlje potrošača",
volume = "74",
number = "1",
pages = "1-16",
doi = "10.2478/acve-2024-0001"
}

Knežević, D., Bošnjaković, D., Dražić, S., Nedić, S., Vujanac, I., Valčić, O., Pantelić, M., Stojiljković, M., Sladojević, Ž.,& Kirovski, D.. (2024). Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers. in Acta Veterinaria, 74(1), 1-16.
https://doi.org/10.2478/acve-2024-0001

Knežević D, Bošnjaković D, Dražić S, Nedić S, Vujanac I, Valčić O, Pantelić M, Stojiljković M, Sladojević Ž, Kirovski D. Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers. in Acta Veterinaria. 2024;74(1):1-16.
doi:10.2478/acve-2024-0001 .

Knežević, Dragan, Bošnjaković, Dušan, Dražić, Slavica, Nedić, Sreten, Vujanac, Ivan, Valčić, Olivera, Pantelić, Marija, Stojiljković, Mojca, Sladojević, Željko, Kirovski, Danijela, "Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers" in Acta Veterinaria, 74, no. 1 (2024):1-16,
https://doi.org/10.2478/acve-2024-0001 . .

TY  - CONF
AU  - Stanić, Vojislav
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Karakašević, Aleksandar
AU  - Pantelić, Marija
AU  - Gerić, Tamara
AU  - Stanković, Srboljub
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12189
AB  - Malondialdehyde (MDA) is the end product of lipid peroxidation and biomarker of free radicals. The aim of this study is to investigate the effect of honey on concentrations of MDA in plasma exposed to a therapeutic dose of radiation. In this experiment animals were divided into two groups: in CONTROL group the animals were given distilled water orally for 28 days, and HONEY group consisted of animals given 1,5 ml/kg honey orally for 28 days. Blood samples of both groups were collected from a rat's tail. All blood samples were exposed to radiation of 2 Gy. Malondialdehyde concentration in plasma was determined using spectrophotometric method. Honey treatment in the blood plasma exposed to a therapeutic dose of 2 Gy significantly decreased MDA level by 30 % compared with control group. The present results indicated that honey had an effect on the levels of MDA in plasma exposed to radiation.
AB  - Malondialdehid (MDA) je krajnji proizvod peroksidacije lipida i biomarker slobodnih radikala. Cilj ovog istraživanja je bio da se ispita uticaj meda na koncentraciju MDA u krvnoj plazmi izloženoj terapijskoj dozi zračenja. U ovom eksperimentu životinje su podeljene u dve grupe: u kontrolnoj grupi životinje su dobijale destilovanu vodu oralno tokom 28 dana, a drugu grupu činile su životinje koje su tokom 28 dana dobijale oralno 1.5 ml/kg meda. Uzorci krvi obe grupe su sakupljeni iz repa pacova. Svi uzorci krvi bili su izloženi zračenju od 2 Gy. Koncentracija MDA u plazmi određena je spektrofotometrijskom metodom. Tretman medom u krvnoj plazmi izložen terapijskoj dozi od 2 Gy značajno je smanjio nivo MDA (30 %) u poređenju sa kontrolnom grupom.
PB  - Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča"
PB  - Društvo za zaštitu od zračenja Srbije i Crne Gore
C3  - 32. simpozijum DZZSCG : zbornik radova
T1  - The Effect of Honey on Malondialdehyde Level in Plasma Exposed to a Therapeutic Dose of Radiation
T1  - Delovanje meda na nivo malondialdehida u plazmi izloženoj terapijskoj dozi zračenja
SP  - 417
EP  - 423
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12189
ER  - 

@conference{
author = "Stanić, Vojislav and Gavrilović, Ljubica and Stojiljković, Vesna and Karakašević, Aleksandar and Pantelić, Marija and Gerić, Tamara and Stanković, Srboljub",
year = "2023",
abstract = "Malondialdehyde (MDA) is the end product of lipid peroxidation and biomarker of free radicals. The aim of this study is to investigate the effect of honey on concentrations of MDA in plasma exposed to a therapeutic dose of radiation. In this experiment animals were divided into two groups: in CONTROL group the animals were given distilled water orally for 28 days, and HONEY group consisted of animals given 1,5 ml/kg honey orally for 28 days. Blood samples of both groups were collected from a rat's tail. All blood samples were exposed to radiation of 2 Gy. Malondialdehyde concentration in plasma was determined using spectrophotometric method. Honey treatment in the blood plasma exposed to a therapeutic dose of 2 Gy significantly decreased MDA level by 30 % compared with control group. The present results indicated that honey had an effect on the levels of MDA in plasma exposed to radiation., Malondialdehid (MDA) je krajnji proizvod peroksidacije lipida i biomarker slobodnih radikala. Cilj ovog istraživanja je bio da se ispita uticaj meda na koncentraciju MDA u krvnoj plazmi izloženoj terapijskoj dozi zračenja. U ovom eksperimentu životinje su podeljene u dve grupe: u kontrolnoj grupi životinje su dobijale destilovanu vodu oralno tokom 28 dana, a drugu grupu činile su životinje koje su tokom 28 dana dobijale oralno 1.5 ml/kg meda. Uzorci krvi obe grupe su sakupljeni iz repa pacova. Svi uzorci krvi bili su izloženi zračenju od 2 Gy. Koncentracija MDA u plazmi određena je spektrofotometrijskom metodom. Tretman medom u krvnoj plazmi izložen terapijskoj dozi od 2 Gy značajno je smanjio nivo MDA (30 %) u poređenju sa kontrolnom grupom.",
publisher = "Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča", Društvo za zaštitu od zračenja Srbije i Crne Gore",
journal = "32. simpozijum DZZSCG : zbornik radova",
title = "The Effect of Honey on Malondialdehyde Level in Plasma Exposed to a Therapeutic Dose of Radiation, Delovanje meda na nivo malondialdehida u plazmi izloženoj terapijskoj dozi zračenja",
pages = "417-423",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12189"
}

Stanić, V., Gavrilović, L., Stojiljković, V., Karakašević, A., Pantelić, M., Gerić, T.,& Stanković, S.. (2023). The Effect of Honey on Malondialdehyde Level in Plasma Exposed to a Therapeutic Dose of Radiation. in 32. simpozijum DZZSCG : zbornik radova
Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča"., 417-423.
https://hdl.handle.net/21.15107/rcub_vinar_12189

Stanić V, Gavrilović L, Stojiljković V, Karakašević A, Pantelić M, Gerić T, Stanković S. The Effect of Honey on Malondialdehyde Level in Plasma Exposed to a Therapeutic Dose of Radiation. in 32. simpozijum DZZSCG : zbornik radova. 2023;:417-423.
https://hdl.handle.net/21.15107/rcub_vinar_12189 .

Stanić, Vojislav, Gavrilović, Ljubica, Stojiljković, Vesna, Karakašević, Aleksandar, Pantelić, Marija, Gerić, Tamara, Stanković, Srboljub, "The Effect of Honey on Malondialdehyde Level in Plasma Exposed to a Therapeutic Dose of Radiation" in 32. simpozijum DZZSCG : zbornik radova (2023):417-423,
https://hdl.handle.net/21.15107/rcub_vinar_12189 .

TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Romić, Snježana
AU  - Zec, Manja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca
AU  - Ivković, Tamara
AU  - Pantelić, Marija
AU  - Kostić, Milan
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11987
AB  - The transport of cations in the cardiomyocytes, crucial for the functioning of the heart, can be affected by walnut diet due to the high content of polyunsaturated fatty acids. Healthy and metabolically compromised rats (drinking 10% fructose solution) were subjected to a diet supplemented with 2.4 g of walnuts for 6 weeks to investigate the effect on proteins involved in cation transport in the heart cells. Fructose increased the level of the a1 subunit of Na+/K+-ATPase and the phosphorylation of extracellular signal-regulated kinase 1/2 in the heart of control and walnut-eating rats, while elevated L-type calcium channel a (LTCCa), sodium–calcium exchanger 1 (NCX1), and Maxi Ka level were observed only in rats that did not consume walnuts. However, walnuts significantly increased the cardiac content of LTCC, NCX1, and Maxi Ka, as well as Kir6.1 and SUR2B subunits of KATP channel, but only in fructose-naive rats. In animals that drank fructose, a significant increasing effect of walnuts was observed only in Akt kinase phosphorylation, which may be a part of the antiarrhythmic mechanism of decreasing cation currents in cardiomyocytes. The walnut diet-induced increase in LTCC and NCX1 expression in healthy rats may indicate intense cardiac calcium turnover, whereas the effect on Kir6.1 and SUR2B subunits suggests stimulation of KATP channel transport in the cardiac vasculature. The effects of walnuts on the cation-handling proteins in the heart, mostly limited to healthy animals, suggest the possible use of a walnut-supplemented diet in the prevention rather than the treatment of cardiological channelopathies.
T2  - Journal of Medicinal Food
T1  - Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats
VL  - 26
IS  - 11
SP  - 849
EP  - 857
DO  - 10.1089/jmf.2022.0157
ER  - 

@article{
author = "Tepavčević, Snežana and Romić, Snježana and Zec, Manja and Ćulafić, Tijana and Stojiljković, Mojca and Ivković, Tamara and Pantelić, Marija and Kostić, Milan and Stanišić, Jelena and Korićanac, Goran",
year = "2023",
abstract = "The transport of cations in the cardiomyocytes, crucial for the functioning of the heart, can be affected by walnut diet due to the high content of polyunsaturated fatty acids. Healthy and metabolically compromised rats (drinking 10% fructose solution) were subjected to a diet supplemented with 2.4 g of walnuts for 6 weeks to investigate the effect on proteins involved in cation transport in the heart cells. Fructose increased the level of the a1 subunit of Na+/K+-ATPase and the phosphorylation of extracellular signal-regulated kinase 1/2 in the heart of control and walnut-eating rats, while elevated L-type calcium channel a (LTCCa), sodium–calcium exchanger 1 (NCX1), and Maxi Ka level were observed only in rats that did not consume walnuts. However, walnuts significantly increased the cardiac content of LTCC, NCX1, and Maxi Ka, as well as Kir6.1 and SUR2B subunits of KATP channel, but only in fructose-naive rats. In animals that drank fructose, a significant increasing effect of walnuts was observed only in Akt kinase phosphorylation, which may be a part of the antiarrhythmic mechanism of decreasing cation currents in cardiomyocytes. The walnut diet-induced increase in LTCC and NCX1 expression in healthy rats may indicate intense cardiac calcium turnover, whereas the effect on Kir6.1 and SUR2B subunits suggests stimulation of KATP channel transport in the cardiac vasculature. The effects of walnuts on the cation-handling proteins in the heart, mostly limited to healthy animals, suggest the possible use of a walnut-supplemented diet in the prevention rather than the treatment of cardiological channelopathies.",
journal = "Journal of Medicinal Food",
title = "Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats",
volume = "26",
number = "11",
pages = "849-857",
doi = "10.1089/jmf.2022.0157"
}

Tepavčević, S., Romić, S., Zec, M., Ćulafić, T., Stojiljković, M., Ivković, T., Pantelić, M., Kostić, M., Stanišić, J.,& Korićanac, G.. (2023). Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats. in Journal of Medicinal Food, 26(11), 849-857.
https://doi.org/10.1089/jmf.2022.0157

Tepavčević S, Romić S, Zec M, Ćulafić T, Stojiljković M, Ivković T, Pantelić M, Kostić M, Stanišić J, Korićanac G. Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats. in Journal of Medicinal Food. 2023;26(11):849-857.
doi:10.1089/jmf.2022.0157 .

Tepavčević, Snežana, Romić, Snježana, Zec, Manja, Ćulafić, Tijana, Stojiljković, Mojca, Ivković, Tamara, Pantelić, Marija, Kostić, Milan, Stanišić, Jelena, Korićanac, Goran, "Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats" in Journal of Medicinal Food, 26, no. 11 (2023):849-857,
https://doi.org/10.1089/jmf.2022.0157 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Đorđević, Ana D.
AU  - Romić, Snježana Đ.
AU  - Pantelić, Marija
AU  - Korićanac, Goran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10356
AB  - A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance, though it is evident that dry cow muscles may contain increased triglyceride content. The current study was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ), glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows. Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin 1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to this disposition.
T2  - Research in Veterinary Science
T1  - Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle
VL  - 150
SP  - 189
EP  - 194
DO  - 10.1016/j.rvsc.2022.04.012
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Vujanac, Ivan and Đorđević, Ana D. and Romić, Snježana Đ. and Pantelić, Marija and Korićanac, Goran",
year = "2022",
abstract = "A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance, though it is evident that dry cow muscles may contain increased triglyceride content. The current study was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ), glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows. Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin 1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to this disposition.",
journal = "Research in Veterinary Science",
title = "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle",
volume = "150",
pages = "189-194",
doi = "10.1016/j.rvsc.2022.04.012"
}

Prodanović, R., Kirovski, D., Vujanac, I., Đorđević, A. D., Romić, S. Đ., Pantelić, M.,& Korićanac, G.. (2022). Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science, 150, 189-194.
https://doi.org/10.1016/j.rvsc.2022.04.012

Prodanović R, Kirovski D, Vujanac I, Đorđević AD, Romić SĐ, Pantelić M, Korićanac G. Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science. 2022;150:189-194.
doi:10.1016/j.rvsc.2022.04.012 .

Prodanović, Radiša, Kirovski, Danijela, Vujanac, Ivan, Đorđević, Ana D., Romić, Snježana Đ., Pantelić, Marija, Korićanac, Goran, "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle" in Research in Veterinary Science, 150 (2022):189-194,
https://doi.org/10.1016/j.rvsc.2022.04.012 . .

TY  - CONF
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stanišić, Jelena
AU  - Ivković, Tamara
AU  - Pantelić, Marija
AU  - Stojiljković, Mojca D.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11009
AB  - Excessive dietary fructose consumption in parallel with limited physical activity contributes to the global increase in prevalence of metabolic disorders. Metabolic syndrome represents a collection of cardiometabolic risk factors that includes obesity, insulin resistance, hypertension, and dyslipidemia, and it is undoubtedly linked to increased risk for two global maladies, type 2 diabetes, and cardiovascular diseases. Fructose-rich diet is accompanied by the development of insulin resistance in the heart, and it could change the use of cardiac energy substrates towards increased fatty acid (FA) uptake, and catabolism. Exercise may be beneficial in prevention and treatment of the metabolic syndrome. The aim of this study was to analyse the impact of low-intensity exercise on protein expression of nuclear transcription factors involved in regulating FA β- oxidation in a heart of fructose fed rats. Male Wistar rats were divided into control group, and two groups that received 10% fructose for 9 weeks, one which was sedentary and one which was additionally exposed to low intensity exercise. The protein expression of important transcriptional regulators of fatty acid β-oxidation PPARα, and FOXO1, and coregulators Lipin1, PGC-1, and SIRT1 are analyzed in cardiac lysate and/or nuclear fraction by Western blot. Gene expression of ACADL, the enzyme that catalyzes the initial step of mitochondrial β-oxidation, was quantified by real-time PCR. Fructose-rich diet decreased nuclear PPARα compared to control. Exercise increased nuclear PPARα, nuclear FOXO1, lysate PGC1, and nuclear Lipin1 in fructose-fed rats compared to sedentary fructose-fed rats. Exercise increased lysate PPARα, lysate and nuclear FOXO1, lysate PGC1, lysate and nuclear SIRT1, and nuclear Lipin1 in fructose-fed rats compared to control. In conclusion, running at low intensity is accompanied by increased expression of key regulators of fatty acid oxidation. The results indicate that exercise achieves its effect by increasing the nuclear content of PPARα, Lipin1, and FOXO1.
PB  - Belgrade : Faculty of Chemistry : Serbian Biochemical Society
C3  - Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
T1  - Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11009
ER  - 

@conference{
author = "Kostić, Milan and Korićanac, Goran and Tepavčević, Snežana and Ćulafić, Tijana and Romić, Snježana Đ. and Stanišić, Jelena and Ivković, Tamara and Pantelić, Marija and Stojiljković, Mojca D.",
year = "2022",
abstract = "Excessive dietary fructose consumption in parallel with limited physical activity contributes to the global increase in prevalence of metabolic disorders. Metabolic syndrome represents a collection of cardiometabolic risk factors that includes obesity, insulin resistance, hypertension, and dyslipidemia, and it is undoubtedly linked to increased risk for two global maladies, type 2 diabetes, and cardiovascular diseases. Fructose-rich diet is accompanied by the development of insulin resistance in the heart, and it could change the use of cardiac energy substrates towards increased fatty acid (FA) uptake, and catabolism. Exercise may be beneficial in prevention and treatment of the metabolic syndrome. The aim of this study was to analyse the impact of low-intensity exercise on protein expression of nuclear transcription factors involved in regulating FA β- oxidation in a heart of fructose fed rats. Male Wistar rats were divided into control group, and two groups that received 10% fructose for 9 weeks, one which was sedentary and one which was additionally exposed to low intensity exercise. The protein expression of important transcriptional regulators of fatty acid β-oxidation PPARα, and FOXO1, and coregulators Lipin1, PGC-1, and SIRT1 are analyzed in cardiac lysate and/or nuclear fraction by Western blot. Gene expression of ACADL, the enzyme that catalyzes the initial step of mitochondrial β-oxidation, was quantified by real-time PCR. Fructose-rich diet decreased nuclear PPARα compared to control. Exercise increased nuclear PPARα, nuclear FOXO1, lysate PGC1, and nuclear Lipin1 in fructose-fed rats compared to sedentary fructose-fed rats. Exercise increased lysate PPARα, lysate and nuclear FOXO1, lysate PGC1, lysate and nuclear SIRT1, and nuclear Lipin1 in fructose-fed rats compared to control. In conclusion, running at low intensity is accompanied by increased expression of key regulators of fatty acid oxidation. The results indicate that exercise achieves its effect by increasing the nuclear content of PPARα, Lipin1, and FOXO1.",
publisher = "Belgrade : Faculty of Chemistry : Serbian Biochemical Society",
journal = "Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia",
title = "Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11009"
}

Kostić, M., Korićanac, G., Tepavčević, S., Ćulafić, T., Romić, S. Đ., Stanišić, J., Ivković, T., Pantelić, M.,& Stojiljković, M. D.. (2022). Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
Belgrade : Faculty of Chemistry : Serbian Biochemical Society., 86.
https://hdl.handle.net/21.15107/rcub_vinar_11009

Kostić M, Korićanac G, Tepavčević S, Ćulafić T, Romić SĐ, Stanišić J, Ivković T, Pantelić M, Stojiljković MD. Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia. 2022;:86.
https://hdl.handle.net/21.15107/rcub_vinar_11009 .

Kostić, Milan, Korićanac, Goran, Tepavčević, Snežana, Ćulafić, Tijana, Romić, Snježana Đ., Stanišić, Jelena, Ivković, Tamara, Pantelić, Marija, Stojiljković, Mojca D., "Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats" in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia (2022):86,
https://hdl.handle.net/21.15107/rcub_vinar_11009 .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Pantelić, Marija
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9959
AB  - Data about the dynamics of noradrenaline (NA) transmission, storage and degradation may be very important for understanding the reduction of functional deficiency of NA and maintaining the stability of NA levels in animals with depressive-like behavior treated with lithium. This study aimed to investigate the effects of mood stabilizer lithium on concentrations of NA in the prefrontal cortex (PFC), as well as behavior rats exposed to chronic restraint stress (CRS). In addition, this study examined the effects of lithium on protein levels of noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and catechol-O-methyltransferase (COMT), as well as the enzyme activity of monoamine oxidase A (MOA) in the PFC of chronically stressed rats. METHODS: The investigated parameters were quantified by Western blot analysis, CAT Research ELISA kits, and an assay of enzyme activity. Also, the forced swim test (FST) was used to assess the behavior of animals. RESULTS: We found that lithium treatment decreased high protein levels of NET and VMAT2, as well as the enzyme activity of MOA in chronically stressed rats to the levels found in unstressed animals. In addition, lithium treatment decreased the concentration of NA (24%) and immobility in animals exposed to CRS. CONCLUSION: Our data confirm that lithium-induced modulation of prefrontal noradrenergic turnover and stabilized the behavior of chronically stressed rats.
T2  - Neuroendocrinology Letters
T1  - Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats
VL  - 42
IS  - 3
SP  - 171
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9959
ER  - 

@article{
author = "Gavrilović, Ljubica and Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pantelić, Marija and Pajović, Snežana B.",
year = "2021",
abstract = "Data about the dynamics of noradrenaline (NA) transmission, storage and degradation may be very important for understanding the reduction of functional deficiency of NA and maintaining the stability of NA levels in animals with depressive-like behavior treated with lithium. This study aimed to investigate the effects of mood stabilizer lithium on concentrations of NA in the prefrontal cortex (PFC), as well as behavior rats exposed to chronic restraint stress (CRS). In addition, this study examined the effects of lithium on protein levels of noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and catechol-O-methyltransferase (COMT), as well as the enzyme activity of monoamine oxidase A (MOA) in the PFC of chronically stressed rats. METHODS: The investigated parameters were quantified by Western blot analysis, CAT Research ELISA kits, and an assay of enzyme activity. Also, the forced swim test (FST) was used to assess the behavior of animals. RESULTS: We found that lithium treatment decreased high protein levels of NET and VMAT2, as well as the enzyme activity of MOA in chronically stressed rats to the levels found in unstressed animals. In addition, lithium treatment decreased the concentration of NA (24%) and immobility in animals exposed to CRS. CONCLUSION: Our data confirm that lithium-induced modulation of prefrontal noradrenergic turnover and stabilized the behavior of chronically stressed rats.",
journal = "Neuroendocrinology Letters",
title = "Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats",
volume = "42",
number = "3",
pages = "171-176",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9959"
}

Gavrilović, L., Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I., Pantelić, M.,& Pajović, S. B.. (2021). Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats. in Neuroendocrinology Letters, 42(3), 171-176.
https://hdl.handle.net/21.15107/rcub_vinar_9959

Gavrilović L, Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Pantelić M, Pajović SB. Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats. in Neuroendocrinology Letters. 2021;42(3):171-176.
https://hdl.handle.net/21.15107/rcub_vinar_9959 .

Gavrilović, Ljubica, Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pantelić, Marija, Pajović, Snežana B., "Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats" in Neuroendocrinology Letters, 42, no. 3 (2021):171-176,
https://hdl.handle.net/21.15107/rcub_vinar_9959 .

TY  - JOUR
AU  - Zec, Manja M.
AU  - Krga, Irena S.
AU  - Takić, Marija M.
AU  - Debeljak-Martačić, Jasmina D.
AU  - Korićanac, Goran
AU  - Ranković, Slavica G.
AU  - Popović, Tamara B.
AU  - Pantelić, Marija
AU  - Glibetić, Maria D.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9731
AB  - Increased dietary, blood, and tissue n-6/n-3 fatty acid ratios are associated with obesity and metabolic syndrome. Due to Westernized dietary patterns, the increasing n-6/n-3 ratio is of growing concern worldwide, and dietary strategies aimed at its lowering are of public health importance. Walnuts are rich in dietary fats, and their consumption promotes cardiometabolic health. This study aimed to examine the effect of 6-week walnut consumption on tissue-specific n-6/n-3 ratio and fatty acid metabolic conversion in fructose-fed rats with a cluster of metabolic disorders. Male Wistar rats were fed a standard diet with or without 10% fructose in drinking water for 9 weeks. Diets of half of the animals were then supplemented with walnuts (2.4 g/day) for 6 weeks, upon which fatty acid profiles were determined in plasma, liver, adipose tissue, and kidney total lipids. Results showed that walnuts induced significant decreases in the n-6/n-3 content of total lipid pool in plasma and examined tissues, irrespective of metabolic burden. Walnut intervention decreased plasma and liver palmitoleic/palmitic, arachidonic/linoleic, and docosahexaenoic/alpha-linolenic acid ratios. It also modulated individual fatty acid levels by reducing arachidonic and palmitic acid and increasing alpha-linolenic, eicosapentaenoic, and docosapentaenoic acid in plasma and most tissues. Our study demonstrated that 6-week consumption of walnuts favorably modulated n-6/n-3 plasma and tissue ratio in male Wistar rats regardless of high-fructose feeding, underscoring the promising potential of walnuts in both prevention and treatment of the metabolic syndrome.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats
VL  - 5
IS  - 43
SP  - 28136
EP  - 28145
DO  - 10.1021/acsomega.0c03784
ER  - 

@article{
author = "Zec, Manja M. and Krga, Irena S. and Takić, Marija M. and Debeljak-Martačić, Jasmina D. and Korićanac, Goran and Ranković, Slavica G. and Popović, Tamara B. and Pantelić, Marija and Glibetić, Maria D.",
year = "2020",
abstract = "Increased dietary, blood, and tissue n-6/n-3 fatty acid ratios are associated with obesity and metabolic syndrome. Due to Westernized dietary patterns, the increasing n-6/n-3 ratio is of growing concern worldwide, and dietary strategies aimed at its lowering are of public health importance. Walnuts are rich in dietary fats, and their consumption promotes cardiometabolic health. This study aimed to examine the effect of 6-week walnut consumption on tissue-specific n-6/n-3 ratio and fatty acid metabolic conversion in fructose-fed rats with a cluster of metabolic disorders. Male Wistar rats were fed a standard diet with or without 10% fructose in drinking water for 9 weeks. Diets of half of the animals were then supplemented with walnuts (2.4 g/day) for 6 weeks, upon which fatty acid profiles were determined in plasma, liver, adipose tissue, and kidney total lipids. Results showed that walnuts induced significant decreases in the n-6/n-3 content of total lipid pool in plasma and examined tissues, irrespective of metabolic burden. Walnut intervention decreased plasma and liver palmitoleic/palmitic, arachidonic/linoleic, and docosahexaenoic/alpha-linolenic acid ratios. It also modulated individual fatty acid levels by reducing arachidonic and palmitic acid and increasing alpha-linolenic, eicosapentaenoic, and docosapentaenoic acid in plasma and most tissues. Our study demonstrated that 6-week consumption of walnuts favorably modulated n-6/n-3 plasma and tissue ratio in male Wistar rats regardless of high-fructose feeding, underscoring the promising potential of walnuts in both prevention and treatment of the metabolic syndrome.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats",
volume = "5",
number = "43",
pages = "28136-28145",
doi = "10.1021/acsomega.0c03784"
}

Zec, M. M., Krga, I. S., Takić, M. M., Debeljak-Martačić, J. D., Korićanac, G., Ranković, S. G., Popović, T. B., Pantelić, M.,& Glibetić, M. D.. (2020). Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats. in ACS Omega
Amer Chemical Soc, Washington., 5(43), 28136-28145.
https://doi.org/10.1021/acsomega.0c03784

Zec MM, Krga IS, Takić MM, Debeljak-Martačić JD, Korićanac G, Ranković SG, Popović TB, Pantelić M, Glibetić MD. Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats. in ACS Omega. 2020;5(43):28136-28145.
doi:10.1021/acsomega.0c03784 .

Zec, Manja M., Krga, Irena S., Takić, Marija M., Debeljak-Martačić, Jasmina D., Korićanac, Goran, Ranković, Slavica G., Popović, Tamara B., Pantelić, Marija, Glibetić, Maria D., "Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats" in ACS Omega, 5, no. 43 (2020):28136-28145,
https://doi.org/10.1021/acsomega.0c03784 . .

TY  - JOUR
AU  - Pantelić, Marija
AU  - Jovanović, Ljubomir J.
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Ćulafić, Tijana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jpn.12655
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7804
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 

@article{
author = "Pantelić, Marija and Jovanović, Ljubomir J. and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Ćulafić, Tijana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}

Pantelić, M., Jovanović, L. J., Prodanović, R., Vujanac, I., Đurić, M., Ćulafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition, 102(1), 41-55.
https://doi.org/10.1111/jpn.12655

Pantelić M, Jovanović LJ, Prodanović R, Vujanac I, Đurić M, Ćulafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55.
doi:10.1111/jpn.12655 .

Pantelić, Marija, Jovanović, Ljubomir J., Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Ćulafić, Tijana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" in Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 . .

TY  - JOUR
AU  - Pantelić, Marija
AU  - Jovanović, Ljubomir J.
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Ćulafić, Tijana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jpn.12655
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7835
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. © 2017 Blackwell Verlag GmbH
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 

@article{
author = "Pantelić, Marija and Jovanović, Ljubomir J. and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Ćulafić, Tijana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. © 2017 Blackwell Verlag GmbH",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}

Pantelić, M., Jovanović, L. J., Prodanović, R., Vujanac, I., Đurić, M., Ćulafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition, 102(1), 41-55.
https://doi.org/10.1111/jpn.12655

Pantelić M, Jovanović LJ, Prodanović R, Vujanac I, Đurić M, Ćulafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55.
doi:10.1111/jpn.12655 .

Pantelić, Marija, Jovanović, Ljubomir J., Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Ćulafić, Tijana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" in Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 . .

TY  - CONF
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Stojiljković, Mirjana
AU  - Ćulafić, Tijana
AU  - Kostić, Mirjana M.
AU  - Romić, Snježana Đ.
AU  - Pantelić, Marija
AU  - Tepavčević, Snežana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7920
C3  - FEBS OPEN BIO
T1  - Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats
VL  - 8
IS  - Supplement 1
SP  - 224
DO  - 10.1002/2211-5463.12453
ER  - 

@conference{
author = "Stanišić, Jelena and Korićanac, Goran and Stojiljković, Mirjana and Ćulafić, Tijana and Kostić, Mirjana M. and Romić, Snježana Đ. and Pantelić, Marija and Tepavčević, Snežana",
year = "2018",
journal = "FEBS OPEN BIO",
title = "Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats",
volume = "8",
number = "Supplement 1",
pages = "224",
doi = "10.1002/2211-5463.12453"
}

Stanišić, J., Korićanac, G., Stojiljković, M., Ćulafić, T., Kostić, M. M., Romić, S. Đ., Pantelić, M.,& Tepavčević, S.. (2018). Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats. in FEBS OPEN BIO, 8(Supplement 1), 224.
https://doi.org/10.1002/2211-5463.12453

Stanišić J, Korićanac G, Stojiljković M, Ćulafić T, Kostić MM, Romić SĐ, Pantelić M, Tepavčević S. Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats. in FEBS OPEN BIO. 2018;8(Supplement 1):224.
doi:10.1002/2211-5463.12453 .

Stanišić, Jelena, Korićanac, Goran, Stojiljković, Mirjana, Ćulafić, Tijana, Kostić, Mirjana M., Romić, Snježana Đ., Pantelić, Marija, Tepavčević, Snežana, "Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats" in FEBS OPEN BIO, 8, no. Supplement 1 (2018):224,
https://doi.org/10.1002/2211-5463.12453 . .

TY  - CONF
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stanišić, Jelena
AU  - Pantelić, Marija
AU  - Stojiljković, Mirjana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7922
C3  - FEBS OPEN BIO
T1  - Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet
VL  - 8
IS  - Supplement 1
SP  - 223
DO  - 10.1002/2211-5463.12453
ER  - 

@conference{
author = "Kostić, Milan and Korićanac, Goran and Tepavčević, Snežana and Ćulafić, Tijana and Romić, Snježana Đ. and Stanišić, Jelena and Pantelić, Marija and Stojiljković, Mirjana",
year = "2018",
journal = "FEBS OPEN BIO",
title = "Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet",
volume = "8",
number = "Supplement 1",
pages = "223",
doi = "10.1002/2211-5463.12453"
}

Kostić, M., Korićanac, G., Tepavčević, S., Ćulafić, T., Romić, S. Đ., Stanišić, J., Pantelić, M.,& Stojiljković, M.. (2018). Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet. in FEBS OPEN BIO, 8(Supplement 1), 223.
https://doi.org/10.1002/2211-5463.12453

Kostić M, Korićanac G, Tepavčević S, Ćulafić T, Romić SĐ, Stanišić J, Pantelić M, Stojiljković M. Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet. in FEBS OPEN BIO. 2018;8(Supplement 1):223.
doi:10.1002/2211-5463.12453 .

Kostić, Milan, Korićanac, Goran, Tepavčević, Snežana, Ćulafić, Tijana, Romić, Snježana Đ., Stanišić, Jelena, Pantelić, Marija, Stojiljković, Mirjana, "Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet" in FEBS OPEN BIO, 8, no. Supplement 1 (2018):223,
https://doi.org/10.1002/2211-5463.12453 . .

TY  - THES
AU  - Pantelić, Marija
PY  - 2018
UR  - http://nardus.mpn.gov.rs/handle/123456789/10606
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6482
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:19217/bdef:Content/download
UR  - https://plus.sr.cobiss.net/opac7/bib/50895375
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8729
AB  - Циљ ове докторске дисертације је био да се испита утицај органског комплекса тровалентног хрома на високомлечне краве у периоду касног гравидитета и ране лактације, као и његов утицај на млечност. Тридесет дана пре очекиваног термина тељења је одабрано 20 крава холштајн расе које су подељене у две једнаке групе контролну (–Cr) и огледну (+Cr). У периоду од 25. дана пре очекиваног термина тељења до 30. дана после тељења, +Cr група крава је свакодневно перорално са јутарњим оброком добијала 10 mg, односно 5 g препарата са органски везаним хромом (0,2 % органског препарата тровалентног хрома везаног у квасцу (Co-Factor III Cr Yeast, Alltech).Интравенски тест толеранције глукозе (IVGTT) је урађен на укупно 12 крава (n=6 крава по групи) четири пута током испитиваног периода и то 28. и 7. дана пре очекиваног термина тељења као и 10. и 30. дана после тељења. Крв је узимана 0., 15., 30., 60., 90., 120. минута теста за одређивање концентрација глукозе и инсулина.Коришћењем података добијених током IVGTT урађена је и кинетика глукозе и инсулина. У узорцима крви узетим 0. минута IVGTT одређена је, поред концентрација глукозе и инсулина, концентрација NEFA (non-esterified fatty acids), BHBА (beta hydroxybutyric acid), укупних протеина, албумина, урее, холестерола, триглицерида (TG), укупног билирубина (TB), Mg, Ca, P и кортизола, као и активности AST и GGT. У циљу испитивања инсулинске резистенције периферних ткива обрачунат је RQUICKI (Revised Quantitative Insulin Sensitivity Check Index) који је показатељ осетљивости ткива на инсулин. Узорци мишићног и масног ткива су узимани биопсијом четири пута у току огледног периода и то 30. и 10. дана пре очекиваног термина тељења као и 7. и 28. дана после тељења, када је урађена ипроцена телесне кондиције (BCS-body condition score)...
AB  - The objective of this study was to investigate the effects of peroral chromium administration on the on high-yielding cows metabolism during the period of late gestation and early lactation, as well as its impact on milk yield. Thirty days before the expected time of parturition, 20 Holstein cows were chosen and divided into two equal groups: control (–Cr) and experimental (+Cr). From day 25 before expected time of parturition up to day 30 after parturition, +Cr received 10 mg of Cr or 5 g per day in the form of chromium-enriched yeast (0.2 % Cr3+ Co-factor III chromium yeast, Alltech).Intravenous glucose tolerance test (IVGTT) was performed on a 12 cows (n = 6 cows per group) four times during the examined period, on days 28th and 7th before the expected time of parturition, as well as on the 10th and 30th day after calving. The blood was taken at 0., 15., 30., 60., 90., 120. minutes of the test to determine the glucose and insulin levels.Using the data obtained during IVGTT, kinetics of glucose and insulin were also performed. The concentrations of non-esterified fatty acids (NEFA), BHBA (beta hydroxybutyric acid), total proteins, albumin, urea, cholesterol, triglycerides (TG), total bilirubin (ТВ), Mg, Ca, P and cortisol, as well as activities of AST and GGT were measured in blood samples taken at minute 0. of IVGTT. In order to examine the peripheral tissue insulin resistance, RQUICKI (Revised Quantitative Insulin Sensitivity Check Index) was calculated. Muscle and adipose tissue samples were taken by biopsy four times during the trial period, 30 and 10 days before the expected time of parturition as well as on the day 7th and 28th day after calving, in time when the body condition score (BCS) was also determined. Hepatic tissue samples were taken twice during the trial period, 10 days before expected time of parturition, and 7 days after calving in order to determine the protein expression of the insulin signal pathway molecules. Milk yield wasrecorded during the first 14 weeks of lactation...
PB  - Универзитет у Београду, Факултет ветеринарске медицине
T2  - Универзитет у Београду
T1  - Утицај пероралне апликације хрома на ендокрини и метаболички статус крава холштајн расе
UR  - https://hdl.handle.net/21.15107/rcub_nardus_10606
ER  - 

@phdthesis{
author = "Pantelić, Marija",
year = "2018",
abstract = "Циљ ове докторске дисертације је био да се испита утицај органског комплекса тровалентног хрома на високомлечне краве у периоду касног гравидитета и ране лактације, као и његов утицај на млечност. Тридесет дана пре очекиваног термина тељења је одабрано 20 крава холштајн расе које су подељене у две једнаке групе контролну (–Cr) и огледну (+Cr). У периоду од 25. дана пре очекиваног термина тељења до 30. дана после тељења, +Cr група крава је свакодневно перорално са јутарњим оброком добијала 10 mg, односно 5 g препарата са органски везаним хромом (0,2 % органског препарата тровалентног хрома везаног у квасцу (Co-Factor III Cr Yeast, Alltech).Интравенски тест толеранције глукозе (IVGTT) је урађен на укупно 12 крава (n=6 крава по групи) четири пута током испитиваног периода и то 28. и 7. дана пре очекиваног термина тељења као и 10. и 30. дана после тељења. Крв је узимана 0., 15., 30., 60., 90., 120. минута теста за одређивање концентрација глукозе и инсулина.Коришћењем података добијених током IVGTT урађена је и кинетика глукозе и инсулина. У узорцима крви узетим 0. минута IVGTT одређена је, поред концентрација глукозе и инсулина, концентрација NEFA (non-esterified fatty acids), BHBА (beta hydroxybutyric acid), укупних протеина, албумина, урее, холестерола, триглицерида (TG), укупног билирубина (TB), Mg, Ca, P и кортизола, као и активности AST и GGT. У циљу испитивања инсулинске резистенције периферних ткива обрачунат је RQUICKI (Revised Quantitative Insulin Sensitivity Check Index) који је показатељ осетљивости ткива на инсулин. Узорци мишићног и масног ткива су узимани биопсијом четири пута у току огледног периода и то 30. и 10. дана пре очекиваног термина тељења као и 7. и 28. дана после тељења, када је урађена ипроцена телесне кондиције (BCS-body condition score)..., The objective of this study was to investigate the effects of peroral chromium administration on the on high-yielding cows metabolism during the period of late gestation and early lactation, as well as its impact on milk yield. Thirty days before the expected time of parturition, 20 Holstein cows were chosen and divided into two equal groups: control (–Cr) and experimental (+Cr). From day 25 before expected time of parturition up to day 30 after parturition, +Cr received 10 mg of Cr or 5 g per day in the form of chromium-enriched yeast (0.2 % Cr3+ Co-factor III chromium yeast, Alltech).Intravenous glucose tolerance test (IVGTT) was performed on a 12 cows (n = 6 cows per group) four times during the examined period, on days 28th and 7th before the expected time of parturition, as well as on the 10th and 30th day after calving. The blood was taken at 0., 15., 30., 60., 90., 120. minutes of the test to determine the glucose and insulin levels.Using the data obtained during IVGTT, kinetics of glucose and insulin were also performed. The concentrations of non-esterified fatty acids (NEFA), BHBA (beta hydroxybutyric acid), total proteins, albumin, urea, cholesterol, triglycerides (TG), total bilirubin (ТВ), Mg, Ca, P and cortisol, as well as activities of AST and GGT were measured in blood samples taken at minute 0. of IVGTT. In order to examine the peripheral tissue insulin resistance, RQUICKI (Revised Quantitative Insulin Sensitivity Check Index) was calculated. Muscle and adipose tissue samples were taken by biopsy four times during the trial period, 30 and 10 days before the expected time of parturition as well as on the day 7th and 28th day after calving, in time when the body condition score (BCS) was also determined. Hepatic tissue samples were taken twice during the trial period, 10 days before expected time of parturition, and 7 days after calving in order to determine the protein expression of the insulin signal pathway molecules. Milk yield wasrecorded during the first 14 weeks of lactation...",
publisher = "Универзитет у Београду, Факултет ветеринарске медицине",
journal = "Универзитет у Београду",
title = "Утицај пероралне апликације хрома на ендокрини и метаболички статус крава холштајн расе",
url = "https://hdl.handle.net/21.15107/rcub_nardus_10606"
}

Pantelić, M.. (2018). Утицај пероралне апликације хрома на ендокрини и метаболички статус крава холштајн расе. in Универзитет у Београду
Универзитет у Београду, Факултет ветеринарске медицине..
https://hdl.handle.net/21.15107/rcub_nardus_10606

Pantelić M. Утицај пероралне апликације хрома на ендокрини и метаболички статус крава холштајн расе. in Универзитет у Београду. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_10606 .

Pantelić, Marija, "Утицај пероралне апликације хрома на ендокрини и метаболички статус крава холштајн расе" in Универзитет у Београду (2018),
https://hdl.handle.net/21.15107/rcub_nardus_10606 .

TY  - JOUR
AU  - Stanisavljević, Andrijana
AU  - Perić, Ivana
AU  - Pantelić, Marija
AU  - Filipović, Dragana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1592
AB  - Olanzapine, an antipsychotic drug, is used to treat depressive disorder, but its effects on the liver, the main site of drug metabolism, still remain elusive. We studied the effects of 3 weeks of olanzapine treatment (7.5 mg/kg per day) on the malondialdehyde (MDA) and protein carbonyl (PCO) contents, protein expression of copper/zinc superoxide dismutase (CuZnSOD), and activity of total superoxide dismutase (SOD), as well as catalase (CAT) protein expression and activity levels in the liver cytosol of rats exposed to 6 weeks of chronic social isolation (CSIS), which causes depressive- and anxiety-like behaviors. Increased cytosolic MDA in CSIS rats (vehicle-or olanzapine-treated) indicated hepatic oxidative stress. Increase in PCO and CAT activity associated with unchanged total SOD activity following CSIS also confirm the presence of oxidative stress. Chronic olanzapine treatment in CSIS prevented increase in PCO without an effect on MDA content. Increased SOD activity in olanzapine-treated (controls and CSIS) groups compared with corresponding vehicle-treated groups and decreased CAT activity in olanzapine-treated CSIS rats compared with vehicle-treated CSIS group was found. The data suggest that chronic olanzapine treatment has a protective effect on hepatic protein oxidation and improves antioxidant defense. The beneficial effects of olanzapine may be due to its free radical scavenging properties and antioxidant activity.
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Olanzapine alleviates oxidative stress in the liver of socially isolated rats
VL  - 95
IS  - 6
SP  - 634
EP  - 640
DO  - 10.1139/cjpp-2016-0598
ER  - 

@article{
author = "Stanisavljević, Andrijana and Perić, Ivana and Pantelić, Marija and Filipović, Dragana",
year = "2017",
abstract = "Olanzapine, an antipsychotic drug, is used to treat depressive disorder, but its effects on the liver, the main site of drug metabolism, still remain elusive. We studied the effects of 3 weeks of olanzapine treatment (7.5 mg/kg per day) on the malondialdehyde (MDA) and protein carbonyl (PCO) contents, protein expression of copper/zinc superoxide dismutase (CuZnSOD), and activity of total superoxide dismutase (SOD), as well as catalase (CAT) protein expression and activity levels in the liver cytosol of rats exposed to 6 weeks of chronic social isolation (CSIS), which causes depressive- and anxiety-like behaviors. Increased cytosolic MDA in CSIS rats (vehicle-or olanzapine-treated) indicated hepatic oxidative stress. Increase in PCO and CAT activity associated with unchanged total SOD activity following CSIS also confirm the presence of oxidative stress. Chronic olanzapine treatment in CSIS prevented increase in PCO without an effect on MDA content. Increased SOD activity in olanzapine-treated (controls and CSIS) groups compared with corresponding vehicle-treated groups and decreased CAT activity in olanzapine-treated CSIS rats compared with vehicle-treated CSIS group was found. The data suggest that chronic olanzapine treatment has a protective effect on hepatic protein oxidation and improves antioxidant defense. The beneficial effects of olanzapine may be due to its free radical scavenging properties and antioxidant activity.",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Olanzapine alleviates oxidative stress in the liver of socially isolated rats",
volume = "95",
number = "6",
pages = "634-640",
doi = "10.1139/cjpp-2016-0598"
}

Stanisavljević, A., Perić, I., Pantelić, M.,& Filipović, D.. (2017). Olanzapine alleviates oxidative stress in the liver of socially isolated rats. in Canadian Journal of Physiology and Pharmacology, 95(6), 634-640.
https://doi.org/10.1139/cjpp-2016-0598

Stanisavljević A, Perić I, Pantelić M, Filipović D. Olanzapine alleviates oxidative stress in the liver of socially isolated rats. in Canadian Journal of Physiology and Pharmacology. 2017;95(6):634-640.
doi:10.1139/cjpp-2016-0598 .

Stanisavljević, Andrijana, Perić, Ivana, Pantelić, Marija, Filipović, Dragana, "Olanzapine alleviates oxidative stress in the liver of socially isolated rats" in Canadian Journal of Physiology and Pharmacology, 95, no. 6 (2017):634-640,
https://doi.org/10.1139/cjpp-2016-0598 . .

TY  - JOUR
AU  - Nedić, Sreten
AU  - Pantelić, Marija
AU  - Vranješ-Đurić, Sanja
AU  - Nedić, Drago
AU  - Jovanović, Ljubomir J.
AU  - Cebulj-Kadunc, Nina
AU  - Kobal, Silvestra
AU  - Snoj, Tomaž
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1894
AB  - Cortisol levels were measured in hair, blood and milk in two different cattle breeds, kept under different breeding conditions and with different genetic merit for milk production. Cows and heifers of Holstein and Busha breeds were selected for the study. Cortisol concentration was determined by immunoassays. Cortisol accumulation was determined in proximal (close to the skin) and distal (far from the skin) segments of the hair shaft. The influence of hair colour and washing prior to extraction and analysis was also examined in order to establish additional factors that may have an impact on hair cortisol concentrations. Concentrations of cortisol determined in the proximal and distal segments of the shaft were significantly higher in Holstein than Busha cows and heifers (P LT 0.05 and P LT 0.01, respectively). In Holstein cows, no significant difference was found between concentrations in black and white hair. In hair washed with isopropanol, cortisol concentration was significantly lower compared to unwashed hair (P LT 0.01). Thus, cortisol concentration in hair varies with the technique of hair processing (washing), but not with colour in Holstein cows. Blood serum cortisol concentrations in Holstein cows and heifers were significantly higher than in Busha cows and heifers, (P LT 0.01 and P LT 0.05, respectively). Milk cortisol in Holstein cows was significantly higher than in Busha cows (P LT 0.05). The higher cortisol concentrations in Holstein cows are assumed to be the result of intensive breeding and physiological adaptation to high milk production.
T2  - Slovenian Veterinary Research
T1  - Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle
VL  - 54
IS  - 4
SP  - 163
EP  - 172
DO  - 10.26873/SVR-398-2017
ER  - 

@article{
author = "Nedić, Sreten and Pantelić, Marija and Vranješ-Đurić, Sanja and Nedić, Drago and Jovanović, Ljubomir J. and Cebulj-Kadunc, Nina and Kobal, Silvestra and Snoj, Tomaž and Kirovski, Danijela",
year = "2017",
abstract = "Cortisol levels were measured in hair, blood and milk in two different cattle breeds, kept under different breeding conditions and with different genetic merit for milk production. Cows and heifers of Holstein and Busha breeds were selected for the study. Cortisol concentration was determined by immunoassays. Cortisol accumulation was determined in proximal (close to the skin) and distal (far from the skin) segments of the hair shaft. The influence of hair colour and washing prior to extraction and analysis was also examined in order to establish additional factors that may have an impact on hair cortisol concentrations. Concentrations of cortisol determined in the proximal and distal segments of the shaft were significantly higher in Holstein than Busha cows and heifers (P LT 0.05 and P LT 0.01, respectively). In Holstein cows, no significant difference was found between concentrations in black and white hair. In hair washed with isopropanol, cortisol concentration was significantly lower compared to unwashed hair (P LT 0.01). Thus, cortisol concentration in hair varies with the technique of hair processing (washing), but not with colour in Holstein cows. Blood serum cortisol concentrations in Holstein cows and heifers were significantly higher than in Busha cows and heifers, (P LT 0.01 and P LT 0.05, respectively). Milk cortisol in Holstein cows was significantly higher than in Busha cows (P LT 0.05). The higher cortisol concentrations in Holstein cows are assumed to be the result of intensive breeding and physiological adaptation to high milk production.",
journal = "Slovenian Veterinary Research",
title = "Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle",
volume = "54",
number = "4",
pages = "163-172",
doi = "10.26873/SVR-398-2017"
}

Nedić, S., Pantelić, M., Vranješ-Đurić, S., Nedić, D., Jovanović, L. J., Cebulj-Kadunc, N., Kobal, S., Snoj, T.,& Kirovski, D.. (2017). Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle. in Slovenian Veterinary Research, 54(4), 163-172.
https://doi.org/10.26873/SVR-398-2017

Nedić S, Pantelić M, Vranješ-Đurić S, Nedić D, Jovanović LJ, Cebulj-Kadunc N, Kobal S, Snoj T, Kirovski D. Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle. in Slovenian Veterinary Research. 2017;54(4):163-172.
doi:10.26873/SVR-398-2017 .

Nedić, Sreten, Pantelić, Marija, Vranješ-Đurić, Sanja, Nedić, Drago, Jovanović, Ljubomir J., Cebulj-Kadunc, Nina, Kobal, Silvestra, Snoj, Tomaž, Kirovski, Danijela, "Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle" in Slovenian Veterinary Research, 54, no. 4 (2017):163-172,
https://doi.org/10.26873/SVR-398-2017 . .

TY  - JOUR
AU  - Jovanović, Ljubomir J.
AU  - Pantelić, Marija
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Tepavčević, Snežana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1808
AB  - The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.
T2  - Biological Trace Element Research
T1  - Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves
VL  - 180
IS  - 2
SP  - 223
EP  - 232
DO  - 10.1007/s12011-017-1007-1
ER  - 

@article{
author = "Jovanović, Ljubomir J. and Pantelić, Marija and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Tepavčević, Snežana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2017",
abstract = "The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.",
journal = "Biological Trace Element Research",
title = "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves",
volume = "180",
number = "2",
pages = "223-232",
doi = "10.1007/s12011-017-1007-1"
}

Jovanović, L. J., Pantelić, M., Prodanović, R., Vujanac, I., Đurić, M., Tepavčević, S., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2017). Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. in Biological Trace Element Research, 180(2), 223-232.
https://doi.org/10.1007/s12011-017-1007-1

Jovanović LJ, Pantelić M, Prodanović R, Vujanac I, Đurić M, Tepavčević S, Vranješ-Đurić S, Korićanac G, Kirovski D. Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. in Biological Trace Element Research. 2017;180(2):223-232.
doi:10.1007/s12011-017-1007-1 .

Jovanović, Ljubomir J., Pantelić, Marija, Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Tepavčević, Snežana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves" in Biological Trace Element Research, 180, no. 2 (2017):223-232,
https://doi.org/10.1007/s12011-017-1007-1 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Vujanac, Ivan
AU  - Đorđević, Ana D.
AU  - Pantelić, Marija
AU  - Romić, Snježana Đ.
AU  - Stanimirovic, Zoran
AU  - Kirovski, Danijela
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1211
AB  - We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 LT = BCS LT = 3.5) and high (4.0 LT = BCS LT = 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Research in Veterinary Science
T1  - Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
VL  - 107
SP  - 16
EP  - 19
DO  - 10.1016/j.rvsc.2016.04.007
ER  - 

@article{
author = "Prodanović, Radiša and Korićanac, Goran and Vujanac, Ivan and Đorđević, Ana D. and Pantelić, Marija and Romić, Snježana Đ. and Stanimirovic, Zoran and Kirovski, Danijela",
year = "2016",
abstract = "We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 LT = BCS LT = 3.5) and high (4.0 LT = BCS LT = 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Research in Veterinary Science",
title = "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression",
volume = "107",
pages = "16-19",
doi = "10.1016/j.rvsc.2016.04.007"
}

Prodanović, R., Korićanac, G., Vujanac, I., Đorđević, A. D., Pantelić, M., Romić, S. Đ., Stanimirovic, Z.,& Kirovski, D.. (2016). Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science, 107, 16-19.
https://doi.org/10.1016/j.rvsc.2016.04.007

Prodanović R, Korićanac G, Vujanac I, Đorđević AD, Pantelić M, Romić SĐ, Stanimirovic Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science. 2016;107:16-19.
doi:10.1016/j.rvsc.2016.04.007 .

Prodanović, Radiša, Korićanac, Goran, Vujanac, Ivan, Đorđević, Ana D., Pantelić, Marija, Romić, Snježana Đ., Stanimirovic, Zoran, Kirovski, Danijela, "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression" in Research in Veterinary Science, 107 (2016):16-19,
https://doi.org/10.1016/j.rvsc.2016.04.007 . .

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Pantelić, Marija
AU  - Tepavčević, Snežana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/913
AB  - Increase in fructose consumption together with decrease in physical activity contributes to the development of metabolic syndrome and consequently cardiovascular diseases. The current study examined the preventive role of exercise on defects in cardiac insulin signaling and function of endothelial nitric oxide synthase (eNOS) in fructose fed rats. Male Wistar rats were divided into control, sedentary fructose (received 10% fructose for 9 weeks) and exercise fructose (additionally exposed to low intensity exercise) groups. Concentration of triglycerides, glucose, insulin and visceral adipose tissue weight were determined to estimate metabolic syndrome development. Expression and/or phosphorylation of cardiac insulin receptor (IR), insulin receptor substrate 1 (IRS1), tyrosine-specific protein phosphatase 1B (PTP1B), Akt, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and eNOS were evaluated. Fructose overload increased visceral adipose tissue, insulin concentration and homeostasis model assessment index. Exercise managed to decrease visceral adiposity and insulin level and to increase insulin sensitivity. Fructose diet increased level of cardiac PTP1B and pIRS1 (Ser307), while levels of IR and ERK1/2, as well as pIRS1 (Tyr 632), pAkt (Ser473, Thr308) and pERK1/2 were decreased. These disturbances were accompanied by reduced phosphorylation of eNOS at Ser1177. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. Low intensity exercise could be considered as efficient treatment of cardiac insulin resistance induced by fructose diet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Molecular and Cellular Endocrinology
T1  - Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet
VL  - 420
IS  - C
SP  - 97
EP  - 104
DO  - 10.1016/j.mce.2015.11.032
ER  - 

@article{
author = "Stanišić, Jelena and Korićanac, Goran and Ćulafić, Tijana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Pantelić, Marija and Tepavčević, Snežana",
year = "2016",
abstract = "Increase in fructose consumption together with decrease in physical activity contributes to the development of metabolic syndrome and consequently cardiovascular diseases. The current study examined the preventive role of exercise on defects in cardiac insulin signaling and function of endothelial nitric oxide synthase (eNOS) in fructose fed rats. Male Wistar rats were divided into control, sedentary fructose (received 10% fructose for 9 weeks) and exercise fructose (additionally exposed to low intensity exercise) groups. Concentration of triglycerides, glucose, insulin and visceral adipose tissue weight were determined to estimate metabolic syndrome development. Expression and/or phosphorylation of cardiac insulin receptor (IR), insulin receptor substrate 1 (IRS1), tyrosine-specific protein phosphatase 1B (PTP1B), Akt, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and eNOS were evaluated. Fructose overload increased visceral adipose tissue, insulin concentration and homeostasis model assessment index. Exercise managed to decrease visceral adiposity and insulin level and to increase insulin sensitivity. Fructose diet increased level of cardiac PTP1B and pIRS1 (Ser307), while levels of IR and ERK1/2, as well as pIRS1 (Tyr 632), pAkt (Ser473, Thr308) and pERK1/2 were decreased. These disturbances were accompanied by reduced phosphorylation of eNOS at Ser1177. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. Low intensity exercise could be considered as efficient treatment of cardiac insulin resistance induced by fructose diet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Molecular and Cellular Endocrinology",
title = "Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet",
volume = "420",
number = "C",
pages = "97-104",
doi = "10.1016/j.mce.2015.11.032"
}

Stanišić, J., Korićanac, G., Ćulafić, T., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Pantelić, M.,& Tepavčević, S.. (2016). Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet. in Molecular and Cellular Endocrinology
Elsevier., 420(C), 97-104.
https://doi.org/10.1016/j.mce.2015.11.032

Stanišić J, Korićanac G, Ćulafić T, Romić SĐ, Stojiljković MD, Kostić M, Pantelić M, Tepavčević S. Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet. in Molecular and Cellular Endocrinology. 2016;420(C):97-104.
doi:10.1016/j.mce.2015.11.032 .

Stanišić, Jelena, Korićanac, Goran, Ćulafić, Tijana, Romić, Snježana Đ., Stojiljković, Mojca D., Kostić, Milan, Pantelić, Marija, Tepavčević, Snežana, "Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet" in Molecular and Cellular Endocrinology, 420, no. C (2016):97-104,
https://doi.org/10.1016/j.mce.2015.11.032 . .

TY  - JOUR
AU  - Ganesan, Minu Karthika
AU  - Jovanović, Miloš
AU  - Šećerov, Bojana Lj.
AU  - Ignjatović, Marija
AU  - Bilban, Martin
AU  - Anđus, Pavle R.
AU  - El Refaei, Amal
AU  - Jung, Gangsoo
AU  - Li, Lin
AU  - Sase, Ajinkya
AU  - Chen, Weiqiang
AU  - Bačić, Goran
AU  - Lubec, Gert
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/133
T2  - Amino Acids
T1  - Erratum to: Radiation protection from whole-body gamma irradiation (6.7 Gy): behavioural effects and brain protein-level changes by an aminothiol compound GL2011 in the Wistar rat (vol 46, pg 1681, 2014)
VL  - 46
IS  - 10
SP  - 2445
EP  - 2445
DO  - 10.1007/s00726-014-1791-2
ER  - 

@article{
author = "Ganesan, Minu Karthika and Jovanović, Miloš and Šećerov, Bojana Lj. and Ignjatović, Marija and Bilban, Martin and Anđus, Pavle R. and El Refaei, Amal and Jung, Gangsoo and Li, Lin and Sase, Ajinkya and Chen, Weiqiang and Bačić, Goran and Lubec, Gert",
year = "2014",
journal = "Amino Acids",
title = "Erratum to: Radiation protection from whole-body gamma irradiation (6.7 Gy): behavioural effects and brain protein-level changes by an aminothiol compound GL2011 in the Wistar rat (vol 46, pg 1681, 2014)",
volume = "46",
number = "10",
pages = "2445-2445",
doi = "10.1007/s00726-014-1791-2"
}

Ganesan, M. K., Jovanović, M., Šećerov, B. Lj., Ignjatović, M., Bilban, M., Anđus, P. R., El Refaei, A., Jung, G., Li, L., Sase, A., Chen, W., Bačić, G.,& Lubec, G.. (2014). Erratum to: Radiation protection from whole-body gamma irradiation (6.7 Gy): behavioural effects and brain protein-level changes by an aminothiol compound GL2011 in the Wistar rat (vol 46, pg 1681, 2014). in Amino Acids, 46(10), 2445-2445.
https://doi.org/10.1007/s00726-014-1791-2

Ganesan MK, Jovanović M, Šećerov BL, Ignjatović M, Bilban M, Anđus PR, El Refaei A, Jung G, Li L, Sase A, Chen W, Bačić G, Lubec G. Erratum to: Radiation protection from whole-body gamma irradiation (6.7 Gy): behavioural effects and brain protein-level changes by an aminothiol compound GL2011 in the Wistar rat (vol 46, pg 1681, 2014). in Amino Acids. 2014;46(10):2445-2445.
doi:10.1007/s00726-014-1791-2 .

Ganesan, Minu Karthika, Jovanović, Miloš, Šećerov, Bojana Lj., Ignjatović, Marija, Bilban, Martin, Anđus, Pavle R., El Refaei, Amal, Jung, Gangsoo, Li, Lin, Sase, Ajinkya, Chen, Weiqiang, Bačić, Goran, Lubec, Gert, "Erratum to: Radiation protection from whole-body gamma irradiation (6.7 Gy): behavioural effects and brain protein-level changes by an aminothiol compound GL2011 in the Wistar rat (vol 46, pg 1681, 2014)" in Amino Acids, 46, no. 10 (2014):2445-2445,
https://doi.org/10.1007/s00726-014-1791-2 . .

TY  - JOUR
AU  - Okić-Đorđević, Ivana
AU  - Trivanović, Drenka
AU  - Jovanović, Miloš
AU  - Ignjatović, Marija
AU  - Šećerov, Bojana Lj.
AU  - Mojovic, Milos
AU  - Bugarski, Diana
AU  - Bačić, Goran
AU  - Anđus, Pavle R.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5935
AB  - Aim To investigate the survival of laboratory rats after irradiation and to study the cellularity of their bone marrow and the multipotential mesenchymal stem cells (BM-MSCs) in groups treated with or without a new thiol-based radioprotector (GM2011) Methods Animals were irradiated by a Cobalt gamma source at 6.7 Gy. Treated animals were given i.p. GM2011 30 minutes before and 3 and 7 hours after irradiation. Controls consisted of sham irradiated animals without treatment and animals treated without irradiation. After 30 days post-irradiation, animals were sacrificed and bone marrow cells were prepared from isolated femurs. A colony forming unit-fibroblast (CFU-F) assay was performed to obtain the number of BM-MSCs. Results In the treated group, 87% of animals survived, compared to only 30% in the non-treated irradiated group. Irradiation induced significant changes in the bone marrow of the treated rats (total bone marrow cellularity was reduced by similar to 60% - from 63 to 28 cells x10(6)/femur and the frequency of the CFU-F per femur by similar to 70% - from 357 to 97), however GL2011 almost completely prevented the suppressive effect observed on day 30 post-irradiation (71 cells x 10(6)/femur and 230 CFU-F/femur). Conclusion Although the irradiation dosage was relatively high, GL2011 acted as a very effective new radioprotector. The recovery of the BN-MSCs and their counts support the effectiveness of the studied radioprotector.
T2  - Croatian Medical Journal
T1  - Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector
VL  - 55
IS  - 1
SP  - 45
EP  - 49
DO  - 10.3325/cmj.2014.55.45
ER  - 

@article{
author = "Okić-Đorđević, Ivana and Trivanović, Drenka and Jovanović, Miloš and Ignjatović, Marija and Šećerov, Bojana Lj. and Mojovic, Milos and Bugarski, Diana and Bačić, Goran and Anđus, Pavle R.",
year = "2014",
abstract = "Aim To investigate the survival of laboratory rats after irradiation and to study the cellularity of their bone marrow and the multipotential mesenchymal stem cells (BM-MSCs) in groups treated with or without a new thiol-based radioprotector (GM2011) Methods Animals were irradiated by a Cobalt gamma source at 6.7 Gy. Treated animals were given i.p. GM2011 30 minutes before and 3 and 7 hours after irradiation. Controls consisted of sham irradiated animals without treatment and animals treated without irradiation. After 30 days post-irradiation, animals were sacrificed and bone marrow cells were prepared from isolated femurs. A colony forming unit-fibroblast (CFU-F) assay was performed to obtain the number of BM-MSCs. Results In the treated group, 87% of animals survived, compared to only 30% in the non-treated irradiated group. Irradiation induced significant changes in the bone marrow of the treated rats (total bone marrow cellularity was reduced by similar to 60% - from 63 to 28 cells x10(6)/femur and the frequency of the CFU-F per femur by similar to 70% - from 357 to 97), however GL2011 almost completely prevented the suppressive effect observed on day 30 post-irradiation (71 cells x 10(6)/femur and 230 CFU-F/femur). Conclusion Although the irradiation dosage was relatively high, GL2011 acted as a very effective new radioprotector. The recovery of the BN-MSCs and their counts support the effectiveness of the studied radioprotector.",
journal = "Croatian Medical Journal",
title = "Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector",
volume = "55",
number = "1",
pages = "45-49",
doi = "10.3325/cmj.2014.55.45"
}

Okić-Đorđević, I., Trivanović, D., Jovanović, M., Ignjatović, M., Šećerov, B. Lj., Mojovic, M., Bugarski, D., Bačić, G.,& Anđus, P. R.. (2014). Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector. in Croatian Medical Journal, 55(1), 45-49.
https://doi.org/10.3325/cmj.2014.55.45

Okić-Đorđević I, Trivanović D, Jovanović M, Ignjatović M, Šećerov BL, Mojovic M, Bugarski D, Bačić G, Anđus PR. Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector. in Croatian Medical Journal. 2014;55(1):45-49.
doi:10.3325/cmj.2014.55.45 .

Okić-Đorđević, Ivana, Trivanović, Drenka, Jovanović, Miloš, Ignjatović, Marija, Šećerov, Bojana Lj., Mojovic, Milos, Bugarski, Diana, Bačić, Goran, Anđus, Pavle R., "Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector" in Croatian Medical Journal, 55, no. 1 (2014):45-49,
https://doi.org/10.3325/cmj.2014.55.45 . .

TY  - JOUR
AU  - Ganesan, Minu Karthika
AU  - Jovanović, Miloš
AU  - Šećerov, Bojana Lj.
AU  - Ignjatović, Marija
AU  - Bilban, Martin
AU  - Anđus, Pavle R.
AU  - El Refaei, Amal
AU  - Jung, Gangsoo
AU  - Li, Lin
AU  - Sase, Ajinkya
AU  - Chen, Weiqiang
AU  - Bačić, Goran
AU  - Lubec, Gert
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6052
AB  - GL2011 is a naturally occurring thiol compound and a series of thiol compounds have been proposed as radioprotectors. Radioprotective efficacy of a triple intraperitoneal dose of GL2011 of 100 mg/kg body weight of Wistar rats, 30 min prior to and 3 and 6 h following irradiation (6.7 Gy) was evaluated. Four groups of animals were used, vehicle-treated non-irradiated (VN), GL2011-treated and irradiated (GI), GL2011-treated and non-irradiated (GN) and vehicle-treated and irradiated (VI) (n = 30 per group). The radioprotective efficacy of GL2011 was determined by measuring 28-day survival and intestinal crypt cell survival. Neuroprotection in terms of behaviour was evaluated using the behavioural observational battery, open field test and elevated plus maze paradigm. An RNA microarray was carried out in order to show differences at the RNA level between VI and VN groups. Brain protein changes were identified using a gel-based proteomics method and major brain receptor complex levels were determined by blue-native gels followed by immunoblotting. 28-Day survival rate in VI was 30 %, in GI survival was 93 %, survival of VN and GN was 100 %. Jejunal crypt cell survival was significantly enhanced in GI. Protein-level changes of peroxiredoxin-5, Mn-superoxide dismutase 2, voltage-dependent anion-selective channel protein 1, septin 5 and dopamine D2 receptor complex levels were paralleling radiation damage and protection. Taken together, the findings demonstrate that GL2011 improves survival rates and jejunal crypt survival, provides partial neuroprotection at the behavioural level and modulates proteins known to be involved in protection against oxidative stress-mediated cell damage.
T2  - Amino Acids
T1  - Radiation protection from whole-body gamma irradiation (6.7 Gy): behavioural effects and brain protein-level changes by an aminothiol compound GL2011 in the Wistar rat
VL  - 46
IS  - 7
SP  - 1681
EP  - 1696
DO  - 10.1007/s00726-014-1728-9
ER  - 

@article{
author = "Ganesan, Minu Karthika and Jovanović, Miloš and Šećerov, Bojana Lj. and Ignjatović, Marija and Bilban, Martin and Anđus, Pavle R. and El Refaei, Amal and Jung, Gangsoo and Li, Lin and Sase, Ajinkya and Chen, Weiqiang and Bačić, Goran and Lubec, Gert",
year = "2014",
abstract = "GL2011 is a naturally occurring thiol compound and a series of thiol compounds have been proposed as radioprotectors. Radioprotective efficacy of a triple intraperitoneal dose of GL2011 of 100 mg/kg body weight of Wistar rats, 30 min prior to and 3 and 6 h following irradiation (6.7 Gy) was evaluated. Four groups of animals were used, vehicle-treated non-irradiated (VN), GL2011-treated and irradiated (GI), GL2011-treated and non-irradiated (GN) and vehicle-treated and irradiated (VI) (n = 30 per group). The radioprotective efficacy of GL2011 was determined by measuring 28-day survival and intestinal crypt cell survival. Neuroprotection in terms of behaviour was evaluated using the behavioural observational battery, open field test and elevated plus maze paradigm. An RNA microarray was carried out in order to show differences at the RNA level between VI and VN groups. Brain protein changes were identified using a gel-based proteomics method and major brain receptor complex levels were determined by blue-native gels followed by immunoblotting. 28-Day survival rate in VI was 30 %, in GI survival was 93 %, survival of VN and GN was 100 %. Jejunal crypt cell survival was significantly enhanced in GI. Protein-level changes of peroxiredoxin-5, Mn-superoxide dismutase 2, voltage-dependent anion-selective channel protein 1, septin 5 and dopamine D2 receptor complex levels were paralleling radiation damage and protection. Taken together, the findings demonstrate that GL2011 improves survival rates and jejunal crypt survival, provides partial neuroprotection at the behavioural level and modulates proteins known to be involved in protection against oxidative stress-mediated cell damage.",
journal = "Amino Acids",
title = "Radiation protection from whole-body gamma irradiation (6.7 Gy): behavioural effects and brain protein-level changes by an aminothiol compound GL2011 in the Wistar rat",
volume = "46",
number = "7",
pages = "1681-1696",
doi = "10.1007/s00726-014-1728-9"
}

Ganesan, M. K., Jovanović, M., Šećerov, B. Lj., Ignjatović, M., Bilban, M., Anđus, P. R., El Refaei, A., Jung, G., Li, L., Sase, A., Chen, W., Bačić, G.,& Lubec, G.. (2014). Radiation protection from whole-body gamma irradiation (6.7 Gy): behavioural effects and brain protein-level changes by an aminothiol compound GL2011 in the Wistar rat. in Amino Acids, 46(7), 1681-1696.
https://doi.org/10.1007/s00726-014-1728-9

Ganesan MK, Jovanović M, Šećerov BL, Ignjatović M, Bilban M, Anđus PR, El Refaei A, Jung G, Li L, Sase A, Chen W, Bačić G, Lubec G. Radiation protection from whole-body gamma irradiation (6.7 Gy): behavioural effects and brain protein-level changes by an aminothiol compound GL2011 in the Wistar rat. in Amino Acids. 2014;46(7):1681-1696.
doi:10.1007/s00726-014-1728-9 .

Ganesan, Minu Karthika, Jovanović, Miloš, Šećerov, Bojana Lj., Ignjatović, Marija, Bilban, Martin, Anđus, Pavle R., El Refaei, Amal, Jung, Gangsoo, Li, Lin, Sase, Ajinkya, Chen, Weiqiang, Bačić, Goran, Lubec, Gert, "Radiation protection from whole-body gamma irradiation (6.7 Gy): behavioural effects and brain protein-level changes by an aminothiol compound GL2011 in the Wistar rat" in Amino Acids, 46, no. 7 (2014):1681-1696,
https://doi.org/10.1007/s00726-014-1728-9 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Korićanac, Goran
AU  - Vranješ-Đurić, Sanja
AU  - Ignjatović, Marija
AU  - Samanc, H.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5701
AB  - The aim of this study was to compare insulin responsevness to acute glucose infusion in cows of Holstein Friesian (HF) and Busa breeds during the peripartal period. Eight cows per each group (HF and Busa), were chosen. At day 7 prior to calving (ante partum) and day 14 after calving (post partum) animals were subjected to a glucose tolerance test (GTT). Blood samples were taken immediately before infusion and 15, 30, 60, 120 and 180 min thereafter Glucose and insulin concentrations were measured in each blood sample, while BHBA and NEFA were measured only in samples taken before the infusion. QUICKY an indicator of insulin resistance in cows was calculated. Basal glycemia did not significantly differ between the breeds. Basal insulinemia was significantly higher in Buga than in HF cows in both examined periods (p LT 0.001, respectively). Basal NEFA levels tended (p =0.06) to be higher in Buga cows compared with those of HF ante partum, and was significantly higher (p LT 0.001) post partum. Basal BHBA was significantly lower in Buga than HF cows in both examined periods (p LT 0.01; p LT 0.001). QUICKI was significantly lower in Buga compared to HF cows both ante partum and post partum periods (p LT 0.001, respectively). Glycemia determined during GTT were higher in Buga than HF cows, both ante partum and post partum, but significantly starting from minute 15 ante partum Le. minute 30 post partum. Insulinemia determined during GTT was significantly lower at min 15, and significantly higher starting from min 90 in Buga than HF cows, both ante partum and postpartum. Results obtained in this study indicate on difference in insulin responsevness to acute glucose infusion between the examined breeds, which is probably a consequence of the difference in the degree of negative energy balance rather than of selection on high milk production. Namely, decreased insulin tissues sensitivity and decreased insulin responsiveness in Buga compared to HF cows is probably the consequence of inadequate energy intake from alimentary sources which leads to enhanced usage of energy from body reserves.
T2  - Acta Veterinaria, Beograd
T1  - Insulin Responses to Acute Glucose Infusions in Busa and Holstein-Friesian Cattle Breed During the Peripartum Period: Comparative Study
VL  - 63
IS  - 4
SP  - 373
EP  - 384
DO  - 10.2298/AVB1304373P
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Vujanac, Ivan and Đurić, Miloje and Korićanac, Goran and Vranješ-Đurić, Sanja and Ignjatović, Marija and Samanc, H.",
year = "2013",
abstract = "The aim of this study was to compare insulin responsevness to acute glucose infusion in cows of Holstein Friesian (HF) and Busa breeds during the peripartal period. Eight cows per each group (HF and Busa), were chosen. At day 7 prior to calving (ante partum) and day 14 after calving (post partum) animals were subjected to a glucose tolerance test (GTT). Blood samples were taken immediately before infusion and 15, 30, 60, 120 and 180 min thereafter Glucose and insulin concentrations were measured in each blood sample, while BHBA and NEFA were measured only in samples taken before the infusion. QUICKY an indicator of insulin resistance in cows was calculated. Basal glycemia did not significantly differ between the breeds. Basal insulinemia was significantly higher in Buga than in HF cows in both examined periods (p LT 0.001, respectively). Basal NEFA levels tended (p =0.06) to be higher in Buga cows compared with those of HF ante partum, and was significantly higher (p LT 0.001) post partum. Basal BHBA was significantly lower in Buga than HF cows in both examined periods (p LT 0.01; p LT 0.001). QUICKI was significantly lower in Buga compared to HF cows both ante partum and post partum periods (p LT 0.001, respectively). Glycemia determined during GTT were higher in Buga than HF cows, both ante partum and post partum, but significantly starting from minute 15 ante partum Le. minute 30 post partum. Insulinemia determined during GTT was significantly lower at min 15, and significantly higher starting from min 90 in Buga than HF cows, both ante partum and postpartum. Results obtained in this study indicate on difference in insulin responsevness to acute glucose infusion between the examined breeds, which is probably a consequence of the difference in the degree of negative energy balance rather than of selection on high milk production. Namely, decreased insulin tissues sensitivity and decreased insulin responsiveness in Buga compared to HF cows is probably the consequence of inadequate energy intake from alimentary sources which leads to enhanced usage of energy from body reserves.",
journal = "Acta Veterinaria, Beograd",
title = "Insulin Responses to Acute Glucose Infusions in Busa and Holstein-Friesian Cattle Breed During the Peripartum Period: Comparative Study",
volume = "63",
number = "4",
pages = "373-384",
doi = "10.2298/AVB1304373P"
}

Prodanović, R., Kirovski, D., Vujanac, I., Đurić, M., Korićanac, G., Vranješ-Đurić, S., Ignjatović, M.,& Samanc, H.. (2013). Insulin Responses to Acute Glucose Infusions in Busa and Holstein-Friesian Cattle Breed During the Peripartum Period: Comparative Study. in Acta Veterinaria, Beograd, 63(4), 373-384.
https://doi.org/10.2298/AVB1304373P

Prodanović R, Kirovski D, Vujanac I, Đurić M, Korićanac G, Vranješ-Đurić S, Ignjatović M, Samanc H. Insulin Responses to Acute Glucose Infusions in Busa and Holstein-Friesian Cattle Breed During the Peripartum Period: Comparative Study. in Acta Veterinaria, Beograd. 2013;63(4):373-384.
doi:10.2298/AVB1304373P .

Prodanović, Radiša, Kirovski, Danijela, Vujanac, Ivan, Đurić, Miloje, Korićanac, Goran, Vranješ-Đurić, Sanja, Ignjatović, Marija, Samanc, H., "Insulin Responses to Acute Glucose Infusions in Busa and Holstein-Friesian Cattle Breed During the Peripartum Period: Comparative Study" in Acta Veterinaria, Beograd, 63, no. 4 (2013):373-384,
https://doi.org/10.2298/AVB1304373P . .

TY  - JOUR
AU  - Milošević, Maja
AU  - Petrovic, Snjezana
AU  - Velickovic, Natasa
AU  - Grković, Ivana
AU  - Ignjatović, Marija
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5131
AB  - Extracellular nucleotides affect female reproductive functions, fertilization, and pregnancy. The aim of this study was to investigate biochemical characteristics of ATP and ADP hydrolysis and identify E-NTPDases in myometrial cell membranes from Wistar albino rats. The apparent K (m) values were 506.4 +/- A 62.1 and 638.8 +/- A 31.3 mu M, with a calculated V (max) (app) of 3,973.0 +/- A 279.5 and 2,853.9 +/- A 79.8 nmol/min/mg for ATP and ADP, respectively. The enzyme activity described here has common properties characteristic for NTPDases: divalent cation dependence; alkaline pH optimum for both substrates, insensitivity to some of classical ATPase inhibitors (ouabain, oligomycine, theophylline, levamisole) and significant inhibition by suramine and high concentration of sodium azides (5 mM). According to similar apparent K-m values for both substrates, the ATP/ADP hydrolysis ratio, and Chevillard competition plot, NTPDase1 is dominant ATP/ADP hydrolyzing enzyme in myometrial cell membranes. RT-PCR analysis revealed expression of three members of ectonucleoside triphosphate diphosphohydrolase family (NTPDase 1, 2, and 8) in rat uterus. These findings may further elucidate the role of NTPDases and ATP in reproductive physiology.
T2  - Molecular and Cellular Biochemistry
T1  - ATP and ADP hydrolysis in cell membranes from rat myometrium
VL  - 371
IS  - 1-2
SP  - 199
EP  - 208
DO  - 10.1007/s11010-012-1436-2
ER  - 

@article{
author = "Milošević, Maja and Petrovic, Snjezana and Velickovic, Natasa and Grković, Ivana and Ignjatović, Marija and Horvat, Anica",
year = "2012",
abstract = "Extracellular nucleotides affect female reproductive functions, fertilization, and pregnancy. The aim of this study was to investigate biochemical characteristics of ATP and ADP hydrolysis and identify E-NTPDases in myometrial cell membranes from Wistar albino rats. The apparent K (m) values were 506.4 +/- A 62.1 and 638.8 +/- A 31.3 mu M, with a calculated V (max) (app) of 3,973.0 +/- A 279.5 and 2,853.9 +/- A 79.8 nmol/min/mg for ATP and ADP, respectively. The enzyme activity described here has common properties characteristic for NTPDases: divalent cation dependence; alkaline pH optimum for both substrates, insensitivity to some of classical ATPase inhibitors (ouabain, oligomycine, theophylline, levamisole) and significant inhibition by suramine and high concentration of sodium azides (5 mM). According to similar apparent K-m values for both substrates, the ATP/ADP hydrolysis ratio, and Chevillard competition plot, NTPDase1 is dominant ATP/ADP hydrolyzing enzyme in myometrial cell membranes. RT-PCR analysis revealed expression of three members of ectonucleoside triphosphate diphosphohydrolase family (NTPDase 1, 2, and 8) in rat uterus. These findings may further elucidate the role of NTPDases and ATP in reproductive physiology.",
journal = "Molecular and Cellular Biochemistry",
title = "ATP and ADP hydrolysis in cell membranes from rat myometrium",
volume = "371",
number = "1-2",
pages = "199-208",
doi = "10.1007/s11010-012-1436-2"
}

Milošević, M., Petrovic, S., Velickovic, N., Grković, I., Ignjatović, M.,& Horvat, A.. (2012). ATP and ADP hydrolysis in cell membranes from rat myometrium. in Molecular and Cellular Biochemistry, 371(1-2), 199-208.
https://doi.org/10.1007/s11010-012-1436-2

Milošević M, Petrovic S, Velickovic N, Grković I, Ignjatović M, Horvat A. ATP and ADP hydrolysis in cell membranes from rat myometrium. in Molecular and Cellular Biochemistry. 2012;371(1-2):199-208.
doi:10.1007/s11010-012-1436-2 .

Milošević, Maja, Petrovic, Snjezana, Velickovic, Natasa, Grković, Ivana, Ignjatović, Marija, Horvat, Anica, "ATP and ADP hydrolysis in cell membranes from rat myometrium" in Molecular and Cellular Biochemistry, 371, no. 1-2 (2012):199-208,
https://doi.org/10.1007/s11010-012-1436-2 . .

TY  - JOUR
AU  - Vujanac, Ivan
AU  - Kirovski, Danijela
AU  - Šamanc, Horea
AU  - Prodanović, Radiša
AU  - Adamović, Milan
AU  - Ignjatović, Marija
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11247
AB  - The objective of this work was to examine the effect of heat stress on glucose and pH values in blood of high-yielding dairy cows in the early stage of lactation, as well as to determine whether the changes in these parameters are interdependent under such conditions. An experiment was performed on high-yielding dairy cows during the summer and the spring periods. Forty cows were selected, twenty each for the two periods under investigation. In the course of the experiment, the temperature humidity index (THI) was determined for the entire period of investigations, and then also the average daily THI, nightmorning THI (average value of hourly THI measured from 22h on the previous day until 10h of the current day), as well as the day-night THI (average value of hourly THI measured during the period from 10h to 22h of the current day). The pH and glucose concentration were determined in blood samples taken in the morning and afternoon of days 30, 60, and 90 of lactation during the spring and summer periods of the investigations. Based on the results for the THI, it was established that the animals were not exposed to the effect of extreme heat stress during the spring period of investigations, while they were periodically exposed to moderate but also extreme heat stress during the summer, in particular in the afternoon hours. It can be concluded from the results obtained for the blood pH that the cows were in respiratory alkalosis during the summer in the morning and afternoon hours on day 30, in the afternoon hours of days 60 and 90 of lactation, as well as in the afternoon on day 90 of lactation during the spring period of investigations. During the summer period, there were no statistically significant differences between the pH value determined in the morning and afternoon hours on day 30 of lactation, while the pH value was significantly higher in the afternoon hours than in the morning hours on days 60 and 90 of lactation. There were no significant differences between the average values for glucose during the spring period of investigations. During the summer, the average afternoon values for glucose on days 60 and 90 of lactation were significantly lower against the morning values. Glucose concentration measured on day 90 was statistically significantly lower than those for days 30 and 60 of lactation, both in the morning and in the afternoon. Glucose concentration on day 90 of lactation during the summer period was significantly lower against glucose concentration during the spring, both in the morning and in the afternoon hours. During the spring period, there was no significant correlation between blood glucemia and pH, while there was a negative correlation between the electrochemical reaction of blood and glucemia during the summer, but which was significant only in the afternoon hours. The decrease in glucemia in the afternoon hours of days 60 and 90 of lactation during the summer period can be explained by the strong influence of alkalosis on insulin secretion that leads to the increased utilization of glucose in peripheral tissues, which is why its concentration in blood is significantly decreased at that time. This is a form of the metabolism shifting under conditions of heat stress, when it uses glucose predominantly as a source of energy in the cells, as its oxidation releases significantly less heat energy in comparison with the combustion of other metabolites.
AB  - Cilj ovog istraživanja je bio da se ispita uticaj toplotnog stresa na vrednosti glikemije i pH krvi kod visokomlečnih krava u ranoj fazi laktacije, kao i da se utvrdi da li su promene ovih parametara u takvim uslovima međusobno zavisne. Ogled je izveden na visokomlečnim kravama tokom leta i proleća. Odabrano je četrdeset krava, po dvadeset za oba perioda ispitivanja. Tokom ogleda računati su satni toplotni indeksi (THI) za ceo period ispitivanja, a zatim i prosečan dnevni THI, noćnojutarnji THI (prosečna vrednost satnih THI izmerenih u periodu od 22h prethodnog dana do 10h ujutro tekućeg dana), kao i dnevnovečernji THI (prosečna vrednost satnih THI izmerenih u periodu od 10 h do 22 h tekućeg dana). U uzorcima krvi uzetim ujutru i posle podne 30, 60. i 90. dana laktacije tokom prolećnog i letnjeg perioda ispitivanja određivan je pH i koncentracija glukoze. Na osnovu rezultata za THI utvrđeno je da životinje u prolećnom periodu ispitivanja nisu bile izložene delovanju izrazitog toplotnog stresa, dok su tokom leta bile periodič no izložene umerenom, ali i izrazitom toplotnom stresu, posebno u poslepodnevnim satima. Iz dobijenih rezultata za pH krvi se može zaključiti da su krave tokom leta bile u respiratornoj alkalozi 30. dana u jutarnjim i popodnevnim, a 60. i 90. dana laktacije u popodnevnim satima, kao i 90. dana laktacije posle podne tokom prolećnog perioda ispitivanja. Tokom leta nije bilo statistički značajne razlike između pH vrednosti određene u jutarnjim i popodnevnim satima 30. dana laktacije, dok je 60. i 90. dana laktacije popodnevna vrednost za pH bila značajno veća u odnosu na jutarnju. Nije bilo značajnih razlika između prosečnih vrednosti glikemija tokom prolećnog perioda ispitivanja. Tokom leta prosečne popodnevne vrednosti glikemija 60. i 90. dana laktacije bile su značajno niže u odnosu na jutarnje. Glikemije izmerene 90. dana bile su statistički značajno niže od glikemija 30. i 60. dana laktacije, kako jutarnje tako i poslepodnevne. Glikemija je 90. dana laktacije u letnjem periodu bila značajno niža u odnosu na glikemiju tokom proleća, kako u jutarnjim, tako i u popodnevnim satima. Tokom proleć nog perioda nije bilo značajne korelacije između glikemije i pH krvi, dok je tokom leta postojala negativna korelacija između elektrohemijske reakcije krvi i glikemije, a koja je bila značajna samo u popodnevnim satima. Smanjenje glikemije u popodnevnim satima 60. i 90. dana laktacije u letnjem periodu može se objasniti snažnim uticajem alkaloze na lučenje insulina, što dovodi do povećanog iskorišćavanja glukoze u perifernim tkivima, zbog čega se njena koncentracija u krvi tada značajno smanjuje. To je vid prestrojavanja metabolizma u uslovima toplotnog stresa kada organizam prevashodno koristi glukozu kao izvor energije u ćelijama jer se njenom oksidacijom oslobađa značajno manje toplotne energije u odnosu na sagorevanje drugih metabolita.
T2  - Veterinarski Glasnik
T1  - Glucose concentration and blood acid-basis status in high-yielding dairy cows during heat stress
T1  - Koncentracija glukoze i acido-bazni status krvi visokomlečnih krava u uslovima toplotnog stresa
VL  - 65
IS  - 5-6
SP  - 297
EP  - 312
DO  - 10.2298/VETGL1106297V
ER  - 

@article{
author = "Vujanac, Ivan and Kirovski, Danijela and Šamanc, Horea and Prodanović, Radiša and Adamović, Milan and Ignjatović, Marija",
year = "2011",
abstract = "The objective of this work was to examine the effect of heat stress on glucose and pH values in blood of high-yielding dairy cows in the early stage of lactation, as well as to determine whether the changes in these parameters are interdependent under such conditions. An experiment was performed on high-yielding dairy cows during the summer and the spring periods. Forty cows were selected, twenty each for the two periods under investigation. In the course of the experiment, the temperature humidity index (THI) was determined for the entire period of investigations, and then also the average daily THI, nightmorning THI (average value of hourly THI measured from 22h on the previous day until 10h of the current day), as well as the day-night THI (average value of hourly THI measured during the period from 10h to 22h of the current day). The pH and glucose concentration were determined in blood samples taken in the morning and afternoon of days 30, 60, and 90 of lactation during the spring and summer periods of the investigations. Based on the results for the THI, it was established that the animals were not exposed to the effect of extreme heat stress during the spring period of investigations, while they were periodically exposed to moderate but also extreme heat stress during the summer, in particular in the afternoon hours. It can be concluded from the results obtained for the blood pH that the cows were in respiratory alkalosis during the summer in the morning and afternoon hours on day 30, in the afternoon hours of days 60 and 90 of lactation, as well as in the afternoon on day 90 of lactation during the spring period of investigations. During the summer period, there were no statistically significant differences between the pH value determined in the morning and afternoon hours on day 30 of lactation, while the pH value was significantly higher in the afternoon hours than in the morning hours on days 60 and 90 of lactation. There were no significant differences between the average values for glucose during the spring period of investigations. During the summer, the average afternoon values for glucose on days 60 and 90 of lactation were significantly lower against the morning values. Glucose concentration measured on day 90 was statistically significantly lower than those for days 30 and 60 of lactation, both in the morning and in the afternoon. Glucose concentration on day 90 of lactation during the summer period was significantly lower against glucose concentration during the spring, both in the morning and in the afternoon hours. During the spring period, there was no significant correlation between blood glucemia and pH, while there was a negative correlation between the electrochemical reaction of blood and glucemia during the summer, but which was significant only in the afternoon hours. The decrease in glucemia in the afternoon hours of days 60 and 90 of lactation during the summer period can be explained by the strong influence of alkalosis on insulin secretion that leads to the increased utilization of glucose in peripheral tissues, which is why its concentration in blood is significantly decreased at that time. This is a form of the metabolism shifting under conditions of heat stress, when it uses glucose predominantly as a source of energy in the cells, as its oxidation releases significantly less heat energy in comparison with the combustion of other metabolites., Cilj ovog istraživanja je bio da se ispita uticaj toplotnog stresa na vrednosti glikemije i pH krvi kod visokomlečnih krava u ranoj fazi laktacije, kao i da se utvrdi da li su promene ovih parametara u takvim uslovima međusobno zavisne. Ogled je izveden na visokomlečnim kravama tokom leta i proleća. Odabrano je četrdeset krava, po dvadeset za oba perioda ispitivanja. Tokom ogleda računati su satni toplotni indeksi (THI) za ceo period ispitivanja, a zatim i prosečan dnevni THI, noćnojutarnji THI (prosečna vrednost satnih THI izmerenih u periodu od 22h prethodnog dana do 10h ujutro tekućeg dana), kao i dnevnovečernji THI (prosečna vrednost satnih THI izmerenih u periodu od 10 h do 22 h tekućeg dana). U uzorcima krvi uzetim ujutru i posle podne 30, 60. i 90. dana laktacije tokom prolećnog i letnjeg perioda ispitivanja određivan je pH i koncentracija glukoze. Na osnovu rezultata za THI utvrđeno je da životinje u prolećnom periodu ispitivanja nisu bile izložene delovanju izrazitog toplotnog stresa, dok su tokom leta bile periodič no izložene umerenom, ali i izrazitom toplotnom stresu, posebno u poslepodnevnim satima. Iz dobijenih rezultata za pH krvi se može zaključiti da su krave tokom leta bile u respiratornoj alkalozi 30. dana u jutarnjim i popodnevnim, a 60. i 90. dana laktacije u popodnevnim satima, kao i 90. dana laktacije posle podne tokom prolećnog perioda ispitivanja. Tokom leta nije bilo statistički značajne razlike između pH vrednosti određene u jutarnjim i popodnevnim satima 30. dana laktacije, dok je 60. i 90. dana laktacije popodnevna vrednost za pH bila značajno veća u odnosu na jutarnju. Nije bilo značajnih razlika između prosečnih vrednosti glikemija tokom prolećnog perioda ispitivanja. Tokom leta prosečne popodnevne vrednosti glikemija 60. i 90. dana laktacije bile su značajno niže u odnosu na jutarnje. Glikemije izmerene 90. dana bile su statistički značajno niže od glikemija 30. i 60. dana laktacije, kako jutarnje tako i poslepodnevne. Glikemija je 90. dana laktacije u letnjem periodu bila značajno niža u odnosu na glikemiju tokom proleća, kako u jutarnjim, tako i u popodnevnim satima. Tokom proleć nog perioda nije bilo značajne korelacije između glikemije i pH krvi, dok je tokom leta postojala negativna korelacija između elektrohemijske reakcije krvi i glikemije, a koja je bila značajna samo u popodnevnim satima. Smanjenje glikemije u popodnevnim satima 60. i 90. dana laktacije u letnjem periodu može se objasniti snažnim uticajem alkaloze na lučenje insulina, što dovodi do povećanog iskorišćavanja glukoze u perifernim tkivima, zbog čega se njena koncentracija u krvi tada značajno smanjuje. To je vid prestrojavanja metabolizma u uslovima toplotnog stresa kada organizam prevashodno koristi glukozu kao izvor energije u ćelijama jer se njenom oksidacijom oslobađa značajno manje toplotne energije u odnosu na sagorevanje drugih metabolita.",
journal = "Veterinarski Glasnik",
title = "Glucose concentration and blood acid-basis status in high-yielding dairy cows during heat stress, Koncentracija glukoze i acido-bazni status krvi visokomlečnih krava u uslovima toplotnog stresa",
volume = "65",
number = "5-6",
pages = "297-312",
doi = "10.2298/VETGL1106297V"
}

Vujanac, I., Kirovski, D., Šamanc, H., Prodanović, R., Adamović, M.,& Ignjatović, M.. (2011). Glucose concentration and blood acid-basis status in high-yielding dairy cows during heat stress. in Veterinarski Glasnik, 65(5-6), 297-312.
https://doi.org/10.2298/VETGL1106297V

Vujanac I, Kirovski D, Šamanc H, Prodanović R, Adamović M, Ignjatović M. Glucose concentration and blood acid-basis status in high-yielding dairy cows during heat stress. in Veterinarski Glasnik. 2011;65(5-6):297-312.
doi:10.2298/VETGL1106297V .

Vujanac, Ivan, Kirovski, Danijela, Šamanc, Horea, Prodanović, Radiša, Adamović, Milan, Ignjatović, Marija, "Glucose concentration and blood acid-basis status in high-yielding dairy cows during heat stress" in Veterinarski Glasnik, 65, no. 5-6 (2011):297-312,
https://doi.org/10.2298/VETGL1106297V . .