TY  - JOUR
AU  - Shin, Wook-Geun
AU  - Sakata, Dousatsu
AU  - Lampe, Nathanael
AU  - Belov, Oleg
AU  - Tran, Ngoc Hoang
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Đorđević, Miloš
AU  - Bernal, Mario A.
AU  - Bordage, Marie-Claude
AU  - Francis, Ziad
AU  - Kyriakou, Ioanna
AU  - Perrot, Yann
AU  - Sasaki, Takashi
AU  - Villagrasa, Carmen
AU  - Guatelli, Susanna
AU  - Breton, Vincent
AU  - Emfietzoglou, Dimitris
AU  - Incerti, Sebastien
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9961
AB  - Accurately modeling the radiobiological mechanisms responsible for the induction of DNA damage remains a major scientific challenge, particularly for understanding the effects of low doses of ionizing radiation on living beings, such as the induction of carcinogenesis. A computational approach based on the Monte Carlo technique to simulate track structures in a biological medium is currently the most reliable method for calculating the early effects induced by ionizing radiation on DNA, the primary cellular target of such effects. The Geant4-DNA Monte Carlo toolkit can simulate not only the physical, but also the physico-chemical and chemical stages of water radiolysis. These stages can be combined with simplified geometric models of biological targets, such as DNA, to assess direct and indirect early DNA damage. In this study, DNA damage induced in a human fibroblast cell was evaluated using Geant4-DNA as a function of incident particle type (gammas, protons, and alphas) and energy. The resulting double-strand break yields as a function of linear energy transfer closely reproduced recent experimental data. Other quantities, such as fragment length distribution, scavengeable damage fraction, and time evolution of damage within an analytical repair model also supported the plausibility of predicting DNA damage using Geant4-DNA.The complete simulation chain application “molecularDNA”, an example for users of Geant4-DNA, will soon be distributed through Geant4.
T2  - Cancers
T2  - Cancers
T1  - A Geant4-DNA Evaluation of Radiation-Induced DNA Damage on a Human Fibroblast
VL  - 13
IS  - 19
SP  - 4940
DO  - 10.3390/cancers13194940
ER  - 

@article{
author = "Shin, Wook-Geun and Sakata, Dousatsu and Lampe, Nathanael and Belov, Oleg and Tran, Ngoc Hoang and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Đorđević, Miloš and Bernal, Mario A. and Bordage, Marie-Claude and Francis, Ziad and Kyriakou, Ioanna and Perrot, Yann and Sasaki, Takashi and Villagrasa, Carmen and Guatelli, Susanna and Breton, Vincent and Emfietzoglou, Dimitris and Incerti, Sebastien",
year = "2021",
abstract = "Accurately modeling the radiobiological mechanisms responsible for the induction of DNA damage remains a major scientific challenge, particularly for understanding the effects of low doses of ionizing radiation on living beings, such as the induction of carcinogenesis. A computational approach based on the Monte Carlo technique to simulate track structures in a biological medium is currently the most reliable method for calculating the early effects induced by ionizing radiation on DNA, the primary cellular target of such effects. The Geant4-DNA Monte Carlo toolkit can simulate not only the physical, but also the physico-chemical and chemical stages of water radiolysis. These stages can be combined with simplified geometric models of biological targets, such as DNA, to assess direct and indirect early DNA damage. In this study, DNA damage induced in a human fibroblast cell was evaluated using Geant4-DNA as a function of incident particle type (gammas, protons, and alphas) and energy. The resulting double-strand break yields as a function of linear energy transfer closely reproduced recent experimental data. Other quantities, such as fragment length distribution, scavengeable damage fraction, and time evolution of damage within an analytical repair model also supported the plausibility of predicting DNA damage using Geant4-DNA.The complete simulation chain application “molecularDNA”, an example for users of Geant4-DNA, will soon be distributed through Geant4.",
journal = "Cancers, Cancers",
title = "A Geant4-DNA Evaluation of Radiation-Induced DNA Damage on a Human Fibroblast",
volume = "13",
number = "19",
pages = "4940",
doi = "10.3390/cancers13194940"
}

Shin, W., Sakata, D., Lampe, N., Belov, O., Tran, N. H., Petrović, I. M., Ristić-Fira, A., Đorđević, M., Bernal, M. A., Bordage, M., Francis, Z., Kyriakou, I., Perrot, Y., Sasaki, T., Villagrasa, C., Guatelli, S., Breton, V., Emfietzoglou, D.,& Incerti, S.. (2021). A Geant4-DNA Evaluation of Radiation-Induced DNA Damage on a Human Fibroblast. in Cancers, 13(19), 4940.
https://doi.org/10.3390/cancers13194940

Shin W, Sakata D, Lampe N, Belov O, Tran NH, Petrović IM, Ristić-Fira A, Đorđević M, Bernal MA, Bordage M, Francis Z, Kyriakou I, Perrot Y, Sasaki T, Villagrasa C, Guatelli S, Breton V, Emfietzoglou D, Incerti S. A Geant4-DNA Evaluation of Radiation-Induced DNA Damage on a Human Fibroblast. in Cancers. 2021;13(19):4940.
doi:10.3390/cancers13194940 .

Shin, Wook-Geun, Sakata, Dousatsu, Lampe, Nathanael, Belov, Oleg, Tran, Ngoc Hoang, Petrović, Ivan M., Ristić-Fira, Aleksandra, Đorđević, Miloš, Bernal, Mario A., Bordage, Marie-Claude, Francis, Ziad, Kyriakou, Ioanna, Perrot, Yann, Sasaki, Takashi, Villagrasa, Carmen, Guatelli, Susanna, Breton, Vincent, Emfietzoglou, Dimitris, Incerti, Sebastien, "A Geant4-DNA Evaluation of Radiation-Induced DNA Damage on a Human Fibroblast" in Cancers, 13, no. 19 (2021):4940,
https://doi.org/10.3390/cancers13194940 . .

TY  - JOUR
AU  - Petringa, Giada
AU  - Calvaruso, Marco
AU  - Conte, Valeria
AU  - Bláha, Pavel
AU  - Bravatà, Valentina
AU  - Cammarata, Francesco Paolo
AU  - Cuttone, Giacomo
AU  - Forte, Giusi Irma
AU  - Keta, Otilija
AU  - Manti, Lorenzo
AU  - Minafra, Luigi
AU  - Petković, Vladana
AU  - Petrović, Ivan M.
AU  - Richiusa, Selene
AU  - Ristić-Fira, Aleksandra
AU  - Russo, Giorgio
AU  - Cirrone, Giuseppe Antonio Pablo
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9958
AB  - CATANA (Centro di AdroTerapia ed Applicazioni Nucleari Avanzate) was the first Italian protontherapy facility dedicated to the treatment of ocular neoplastic pathologies. It is in operation at the LNS Laboratories of the Italian Institute for Nuclear Physics (INFN-LNS) and to date, 500 patients have been successfully treated. Even though proton therapy has demonstrated success in clinical settings, there is still a need for more accurate models because they are crucial for the estimation of clinically relevant RBE values. Since RBE can vary depending on several physical and biological parameters, there is a clear need for more experimental data to generate predictions. Establishing a database of cell survival experiments is therefore useful to accurately predict the effects of irradiations on both cancerous and normal tissue. The main aim of this work was to compare RBE values obtained from in-vitro experimental data with predictions made by the LEM II (Local Effect Model), Monte Carlo approaches, and semi-empirical models based on LET experimental measurements. For this purpose, the 92.1 uveal melanoma and ARPE-19 cells derived from normal retinal pigmented epithelium were selected and irradiated in the middle of clinical SOBP of the CATANA proton therapy facility. The remarkable results show the potentiality of using microdosimetric spectrum, Monte Carlo simulations and LEM model to predict not only the RBE but also the survival curves.
T2  - Applied Sciences
T1  - Radiobiological Outcomes, Microdosimetric Evaluations and Monte Carlo Predictions in Eye Proton Therapy
VL  - 11
IS  - 19
SP  - 8822
DO  - 10.3390/app11198822
ER  - 

@article{
author = "Petringa, Giada and Calvaruso, Marco and Conte, Valeria and Bláha, Pavel and Bravatà, Valentina and Cammarata, Francesco Paolo and Cuttone, Giacomo and Forte, Giusi Irma and Keta, Otilija and Manti, Lorenzo and Minafra, Luigi and Petković, Vladana and Petrović, Ivan M. and Richiusa, Selene and Ristić-Fira, Aleksandra and Russo, Giorgio and Cirrone, Giuseppe Antonio Pablo",
year = "2021",
abstract = "CATANA (Centro di AdroTerapia ed Applicazioni Nucleari Avanzate) was the first Italian protontherapy facility dedicated to the treatment of ocular neoplastic pathologies. It is in operation at the LNS Laboratories of the Italian Institute for Nuclear Physics (INFN-LNS) and to date, 500 patients have been successfully treated. Even though proton therapy has demonstrated success in clinical settings, there is still a need for more accurate models because they are crucial for the estimation of clinically relevant RBE values. Since RBE can vary depending on several physical and biological parameters, there is a clear need for more experimental data to generate predictions. Establishing a database of cell survival experiments is therefore useful to accurately predict the effects of irradiations on both cancerous and normal tissue. The main aim of this work was to compare RBE values obtained from in-vitro experimental data with predictions made by the LEM II (Local Effect Model), Monte Carlo approaches, and semi-empirical models based on LET experimental measurements. For this purpose, the 92.1 uveal melanoma and ARPE-19 cells derived from normal retinal pigmented epithelium were selected and irradiated in the middle of clinical SOBP of the CATANA proton therapy facility. The remarkable results show the potentiality of using microdosimetric spectrum, Monte Carlo simulations and LEM model to predict not only the RBE but also the survival curves.",
journal = "Applied Sciences",
title = "Radiobiological Outcomes, Microdosimetric Evaluations and Monte Carlo Predictions in Eye Proton Therapy",
volume = "11",
number = "19",
pages = "8822",
doi = "10.3390/app11198822"
}

Petringa, G., Calvaruso, M., Conte, V., Bláha, P., Bravatà, V., Cammarata, F. P., Cuttone, G., Forte, G. I., Keta, O., Manti, L., Minafra, L., Petković, V., Petrović, I. M., Richiusa, S., Ristić-Fira, A., Russo, G.,& Cirrone, G. A. P.. (2021). Radiobiological Outcomes, Microdosimetric Evaluations and Monte Carlo Predictions in Eye Proton Therapy. in Applied Sciences, 11(19), 8822.
https://doi.org/10.3390/app11198822

Petringa G, Calvaruso M, Conte V, Bláha P, Bravatà V, Cammarata FP, Cuttone G, Forte GI, Keta O, Manti L, Minafra L, Petković V, Petrović IM, Richiusa S, Ristić-Fira A, Russo G, Cirrone GAP. Radiobiological Outcomes, Microdosimetric Evaluations and Monte Carlo Predictions in Eye Proton Therapy. in Applied Sciences. 2021;11(19):8822.
doi:10.3390/app11198822 .

Petringa, Giada, Calvaruso, Marco, Conte, Valeria, Bláha, Pavel, Bravatà, Valentina, Cammarata, Francesco Paolo, Cuttone, Giacomo, Forte, Giusi Irma, Keta, Otilija, Manti, Lorenzo, Minafra, Luigi, Petković, Vladana, Petrović, Ivan M., Richiusa, Selene, Ristić-Fira, Aleksandra, Russo, Giorgio, Cirrone, Giuseppe Antonio Pablo, "Radiobiological Outcomes, Microdosimetric Evaluations and Monte Carlo Predictions in Eye Proton Therapy" in Applied Sciences, 11, no. 19 (2021):8822,
https://doi.org/10.3390/app11198822 . .

TY  - JOUR
AU  - Keta, Otilija
AU  - Petković, Vladana
AU  - Cirrone, Pablo
AU  - Petringa, Giada
AU  - Cuttone, Giacomo
AU  - Sakata, Dousatsu
AU  - Shin, Wook-Geun
AU  - Incerti, Sebastien
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9875
AB  - Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation and disappearance of DNA double-strand breaks (DNA DSB) as a function of LET and time. Comparisons with conventional γ-rays and high LET carbon ions were also performed.Materials and Methods In the present work, we performed immunofluorescence-based assay to determine the amount of DNA DSB induced by different LET values along the 62 MeV therapeutic proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma and HTB177 non-small lung cancer cells. Time dependence of foci formation was followed as well. To determine irradiation positions, corresponding to the desired LET values, numerical simulations were carried out using Geant4 toolkit. We compared γ-H2AX foci persistence after irradiations with protons to that of γ-rays and carbon ions.Results With the rise of LET values along the therapeutic proton SOBP, the increase of γ-H2AX foci number is detected in the three cell lines up to the distal end of the SOBP, while there is a decrease on its distal fall-off part. With the prolonged incubation time, the number of foci gradually drops tending to attain the residual level. For the maximum number of DNA DSB, irradiation with protons attain higher level than that of γ-rays. Carbon ions produce more DNA DSB than protons but not substantially. The number of residual foci produced by γ-rays is significantly lower than that of protons and particularly carbon ions. Carbon ions do not produce considerably higher number of foci than protons, as it could be expected due to their physical properties.Conclusions In situ visualization of γ-H2AX foci reveal creation of more lesions in the three cell lines by clinically relevant proton SOBP than γ-rays. The lack of significant differences in the number of γ-H2AX foci between the proton and carbon ion-irradiated samples suggests an increased complexity of DNA lesions and slower repair kinetics after carbon ions compared to protons. For all three irradiation types, there is no major difference between the three cell lines shortly after irradiations, while later on, the formation of residual foci starts to express the inherent nature of tested cells, therefore increasing discrepancy between them.
T2  - International Journal of Radiation Biology
T1  - DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time
VL  - 97
IS  - 9
SP  - 1229
EP  - 1240
DO  - 10.1080/09553002.2021.1948140
ER  - 

@article{
author = "Keta, Otilija and Petković, Vladana and Cirrone, Pablo and Petringa, Giada and Cuttone, Giacomo and Sakata, Dousatsu and Shin, Wook-Geun and Incerti, Sebastien and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2021",
abstract = "Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation and disappearance of DNA double-strand breaks (DNA DSB) as a function of LET and time. Comparisons with conventional γ-rays and high LET carbon ions were also performed.Materials and Methods In the present work, we performed immunofluorescence-based assay to determine the amount of DNA DSB induced by different LET values along the 62 MeV therapeutic proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma and HTB177 non-small lung cancer cells. Time dependence of foci formation was followed as well. To determine irradiation positions, corresponding to the desired LET values, numerical simulations were carried out using Geant4 toolkit. We compared γ-H2AX foci persistence after irradiations with protons to that of γ-rays and carbon ions.Results With the rise of LET values along the therapeutic proton SOBP, the increase of γ-H2AX foci number is detected in the three cell lines up to the distal end of the SOBP, while there is a decrease on its distal fall-off part. With the prolonged incubation time, the number of foci gradually drops tending to attain the residual level. For the maximum number of DNA DSB, irradiation with protons attain higher level than that of γ-rays. Carbon ions produce more DNA DSB than protons but not substantially. The number of residual foci produced by γ-rays is significantly lower than that of protons and particularly carbon ions. Carbon ions do not produce considerably higher number of foci than protons, as it could be expected due to their physical properties.Conclusions In situ visualization of γ-H2AX foci reveal creation of more lesions in the three cell lines by clinically relevant proton SOBP than γ-rays. The lack of significant differences in the number of γ-H2AX foci between the proton and carbon ion-irradiated samples suggests an increased complexity of DNA lesions and slower repair kinetics after carbon ions compared to protons. For all three irradiation types, there is no major difference between the three cell lines shortly after irradiations, while later on, the formation of residual foci starts to express the inherent nature of tested cells, therefore increasing discrepancy between them.",
journal = "International Journal of Radiation Biology",
title = "DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time",
volume = "97",
number = "9",
pages = "1229-1240",
doi = "10.1080/09553002.2021.1948140"
}

Keta, O., Petković, V., Cirrone, P., Petringa, G., Cuttone, G., Sakata, D., Shin, W., Incerti, S., Petrović, I. M.,& Ristić-Fira, A.. (2021). DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time. in International Journal of Radiation Biology, 97(9), 1229-1240.
https://doi.org/10.1080/09553002.2021.1948140

Keta O, Petković V, Cirrone P, Petringa G, Cuttone G, Sakata D, Shin W, Incerti S, Petrović IM, Ristić-Fira A. DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time. in International Journal of Radiation Biology. 2021;97(9):1229-1240.
doi:10.1080/09553002.2021.1948140 .

Keta, Otilija, Petković, Vladana, Cirrone, Pablo, Petringa, Giada, Cuttone, Giacomo, Sakata, Dousatsu, Shin, Wook-Geun, Incerti, Sebastien, Petrović, Ivan M., Ristić-Fira, Aleksandra, "DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time" in International Journal of Radiation Biology, 97, no. 9 (2021):1229-1240,
https://doi.org/10.1080/09553002.2021.1948140 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Deljanin, Milena
AU  - Petković, Vladana
AU  - Zdunić, Gordana
AU  - Janković, Teodora
AU  - Živković, Jelena
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Šavikin, Katarina
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8996
AB  - The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.
T2  - Records of Natural Products
T1  - Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines
VL  - 14
IS  - 5
SP  - 326
EP  - 339
DO  - 10.25135/rnp.170.19.11.1477
ER  - 

@article{
author = "Keta, Otilija D. and Deljanin, Milena and Petković, Vladana and Zdunić, Gordana and Janković, Teodora and Živković, Jelena and Ristić-Fira, Aleksandra and Petrović, Ivan M. and Šavikin, Katarina",
year = "2020",
abstract = "The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.",
journal = "Records of Natural Products",
title = "Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines",
volume = "14",
number = "5",
pages = "326-339",
doi = "10.25135/rnp.170.19.11.1477"
}

Keta, O. D., Deljanin, M., Petković, V., Zdunić, G., Janković, T., Živković, J., Ristić-Fira, A., Petrović, I. M.,& Šavikin, K.. (2020). Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines. in Records of Natural Products, 14(5), 326-339.
https://doi.org/10.25135/rnp.170.19.11.1477

Keta OD, Deljanin M, Petković V, Zdunić G, Janković T, Živković J, Ristić-Fira A, Petrović IM, Šavikin K. Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines. in Records of Natural Products. 2020;14(5):326-339.
doi:10.25135/rnp.170.19.11.1477 .

Keta, Otilija D., Deljanin, Milena, Petković, Vladana, Zdunić, Gordana, Janković, Teodora, Živković, Jelena, Ristić-Fira, Aleksandra, Petrović, Ivan M., Šavikin, Katarina, "Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines" in Records of Natural Products, 14, no. 5 (2020):326-339,
https://doi.org/10.25135/rnp.170.19.11.1477 . .

TY  - JOUR
AU  - Sakata, Dousatsu
AU  - Belov, Oleg
AU  - Bordage, Marie-Claude
AU  - Emfietzoglou, Dimitris
AU  - Guatelli, Susanna
AU  - Inaniwa, Taku
AU  - Ivanchenko, Vladimir
AU  - Karamitros, Mathieu
AU  - Kyriakou, Ioanna
AU  - Lampe, Nathanael
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Shin, Wook-Geun
AU  - Incerti, Sebastien
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9751
AB  - Ionising radiation induced DNA damage and subsequent biological responses to it depend on the radiation's track-structure and its energy loss distribution pattern. To investigate the underlying biological mechanisms involved in such complex system, there is need of predicting biological response by integrated Monte Carlo (MC) simulations across physics, chemistry and biology. Hence, in this work, we have developed an application using the open source Geant4-DNA toolkit to propose a realistic "fully integrated" MC simulation to calculate both early DNA damage and subsequent biological responses with time. We had previously developed an application allowing simulations of radiation induced early DNA damage on a naked cell nucleus model. In the new version presented in this work, we have developed three additional important features: (1) modeling of a realistic cell geometry, (2) inclusion of a biological repair model, (3) refinement of DNA damage parameters for direct damage and indirect damage scoring. The simulation results are validated with experimental data in terms of Single Strand Break (SSB) yields for plasmid and Double Strand Break (DSB) yields for plasmid/human cell. In addition, the yields of indirect DSBs are compatible with the experimental scavengeable damage fraction. The simulation application also demonstrates agreement with experimental data of gamma -H2AX yields for gamma ray irradiation. Using this application, it is now possible to predict biological response along time through track-structure MC simulations.
T2  - Scientific Reports
T1  - Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA
VL  - 10
IS  - 1
SP  - 20788
DO  - 10.1038/s41598-020-75982-x
ER  - 

@article{
author = "Sakata, Dousatsu and Belov, Oleg and Bordage, Marie-Claude and Emfietzoglou, Dimitris and Guatelli, Susanna and Inaniwa, Taku and Ivanchenko, Vladimir and Karamitros, Mathieu and Kyriakou, Ioanna and Lampe, Nathanael and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Shin, Wook-Geun and Incerti, Sebastien",
year = "2020",
abstract = "Ionising radiation induced DNA damage and subsequent biological responses to it depend on the radiation's track-structure and its energy loss distribution pattern. To investigate the underlying biological mechanisms involved in such complex system, there is need of predicting biological response by integrated Monte Carlo (MC) simulations across physics, chemistry and biology. Hence, in this work, we have developed an application using the open source Geant4-DNA toolkit to propose a realistic "fully integrated" MC simulation to calculate both early DNA damage and subsequent biological responses with time. We had previously developed an application allowing simulations of radiation induced early DNA damage on a naked cell nucleus model. In the new version presented in this work, we have developed three additional important features: (1) modeling of a realistic cell geometry, (2) inclusion of a biological repair model, (3) refinement of DNA damage parameters for direct damage and indirect damage scoring. The simulation results are validated with experimental data in terms of Single Strand Break (SSB) yields for plasmid and Double Strand Break (DSB) yields for plasmid/human cell. In addition, the yields of indirect DSBs are compatible with the experimental scavengeable damage fraction. The simulation application also demonstrates agreement with experimental data of gamma -H2AX yields for gamma ray irradiation. Using this application, it is now possible to predict biological response along time through track-structure MC simulations.",
journal = "Scientific Reports",
title = "Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA",
volume = "10",
number = "1",
pages = "20788",
doi = "10.1038/s41598-020-75982-x"
}

Sakata, D., Belov, O., Bordage, M., Emfietzoglou, D., Guatelli, S., Inaniwa, T., Ivanchenko, V., Karamitros, M., Kyriakou, I., Lampe, N., Petrović, I. M., Ristić-Fira, A., Shin, W.,& Incerti, S.. (2020). Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA. in Scientific Reports, 10(1), 20788.
https://doi.org/10.1038/s41598-020-75982-x

Sakata D, Belov O, Bordage M, Emfietzoglou D, Guatelli S, Inaniwa T, Ivanchenko V, Karamitros M, Kyriakou I, Lampe N, Petrović IM, Ristić-Fira A, Shin W, Incerti S. Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA. in Scientific Reports. 2020;10(1):20788.
doi:10.1038/s41598-020-75982-x .

Sakata, Dousatsu, Belov, Oleg, Bordage, Marie-Claude, Emfietzoglou, Dimitris, Guatelli, Susanna, Inaniwa, Taku, Ivanchenko, Vladimir, Karamitros, Mathieu, Kyriakou, Ioanna, Lampe, Nathanael, Petrović, Ivan M., Ristić-Fira, Aleksandra, Shin, Wook-Geun, Incerti, Sebastien, "Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA" in Scientific Reports, 10, no. 1 (2020):20788,
https://doi.org/10.1038/s41598-020-75982-x . .

TY  - JOUR
AU  - Scholz, Michael
AU  - Friedrich, Thomas
AU  - Magrin, Giulio
AU  - Colautti, Paolo
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9614
AB  - The specific advantages of ion beams for application in tumor therapy are attributed to their different macroscopic and microscopic energy deposition pattern as compared to conventional photon radiation. On the macroscopic scale, the inverted dose profile with a Bragg peak and small lateral scattering allow a better conformation of the dose to the tumor. On the microscopic scale, the localized energy deposition around the trajectory of the particles leads to an enhanced biological effectiveness, typically expressed in terms of the relative biological effectiveness (RBE). Experimental investigations reveal complex dependencies of RBE on many physical and biological parameters, as e.g., ion species, dose, position in the field and cell or tissue type. In order to complement the experimental work, different approaches are used for the characterization of the specific physical and biological properties of ion beams. In a set of two papers, which are linked by activities within a European HORIZON 2020 project about nuclear science and application (ENSAR2), we describe recent developments in two fields playing a key role in characterizing the increased biological effectiveness. These comprise the biophysical modeling of RBE and the microdosimetric measurements in complex radiation fields. This first paper gives a brief introduction into these fields and then focuses on aspects of biophysical modeling of RBE, specifically on semi-empirical approaches that are currently used in treatment planning for ion beam therapy. It summarizes the status and recent developments of the Local Effect Model (LEM) and its conceptual framework and shows examples of model validation using recent experimental data. The model is compared to other approaches, e.g., to the Microdosimetric-Kinetic Model (MKM), that builds the bridge to the experimental microdosimetric work. © Copyright © 2020 Scholz, Friedrich, Magrin, Colautti, Ristić-Fira and Petrović.
T2  - Frontiers in Physics
T1  - Characterizing Radiation Effectiveness in Ion Beam Therapy Part I: Introduction and Biophysical Modeling of RBE Using the LEMIV
VL  - 8
DO  - 10.3389/fphy.2020.00272
ER  - 

@article{
author = "Scholz, Michael and Friedrich, Thomas and Magrin, Giulio and Colautti, Paolo and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2020",
abstract = "The specific advantages of ion beams for application in tumor therapy are attributed to their different macroscopic and microscopic energy deposition pattern as compared to conventional photon radiation. On the macroscopic scale, the inverted dose profile with a Bragg peak and small lateral scattering allow a better conformation of the dose to the tumor. On the microscopic scale, the localized energy deposition around the trajectory of the particles leads to an enhanced biological effectiveness, typically expressed in terms of the relative biological effectiveness (RBE). Experimental investigations reveal complex dependencies of RBE on many physical and biological parameters, as e.g., ion species, dose, position in the field and cell or tissue type. In order to complement the experimental work, different approaches are used for the characterization of the specific physical and biological properties of ion beams. In a set of two papers, which are linked by activities within a European HORIZON 2020 project about nuclear science and application (ENSAR2), we describe recent developments in two fields playing a key role in characterizing the increased biological effectiveness. These comprise the biophysical modeling of RBE and the microdosimetric measurements in complex radiation fields. This first paper gives a brief introduction into these fields and then focuses on aspects of biophysical modeling of RBE, specifically on semi-empirical approaches that are currently used in treatment planning for ion beam therapy. It summarizes the status and recent developments of the Local Effect Model (LEM) and its conceptual framework and shows examples of model validation using recent experimental data. The model is compared to other approaches, e.g., to the Microdosimetric-Kinetic Model (MKM), that builds the bridge to the experimental microdosimetric work. © Copyright © 2020 Scholz, Friedrich, Magrin, Colautti, Ristić-Fira and Petrović.",
journal = "Frontiers in Physics",
title = "Characterizing Radiation Effectiveness in Ion Beam Therapy Part I: Introduction and Biophysical Modeling of RBE Using the LEMIV",
volume = "8",
doi = "10.3389/fphy.2020.00272"
}

Scholz, M., Friedrich, T., Magrin, G., Colautti, P., Ristić-Fira, A.,& Petrović, I. M.. (2020). Characterizing Radiation Effectiveness in Ion Beam Therapy Part I: Introduction and Biophysical Modeling of RBE Using the LEMIV. in Frontiers in Physics, 8.
https://doi.org/10.3389/fphy.2020.00272

Scholz M, Friedrich T, Magrin G, Colautti P, Ristić-Fira A, Petrović IM. Characterizing Radiation Effectiveness in Ion Beam Therapy Part I: Introduction and Biophysical Modeling of RBE Using the LEMIV. in Frontiers in Physics. 2020;8.
doi:10.3389/fphy.2020.00272 .

Scholz, Michael, Friedrich, Thomas, Magrin, Giulio, Colautti, Paolo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Characterizing Radiation Effectiveness in Ion Beam Therapy Part I: Introduction and Biophysical Modeling of RBE Using the LEMIV" in Frontiers in Physics, 8 (2020),
https://doi.org/10.3389/fphy.2020.00272 . .

TY  - JOUR
AU  - Petković, Vladana
AU  - Keta, Otilija D.
AU  - Vidosavljević, Marija Z.
AU  - Incerti, Sebastien
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8414
AB  - Purpose: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high linear energy transfer (LET) radiations used in hadron therapy, i.e. protons and carbon ions. Materials and methods: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers. Biological response to irradiation is evaluated through clonogenic cell survival, immunocytochemical and cell cycle analysis, as well as expression of proteins involved in DNA damage response. Results: Examined cell lines reveal similar level of radioresistance. Application of scavengers leads to the rise of cell survival and decreases the number of DNA double strand breaks in irradiated cells. Differences in cell cycle and protein expression between the two cell lines are probably caused by different DNA damage repair mechanisms that are activated. Conclusion: The obtained results show that DMSO and glycerol have good scavenging capacity, and may be used to minimize DNA damage induced by free radicals. Therefore, they will be used as the reference for comparison with high LET irradiations, as well as good experimental data suitable for validation of numerical simulations. © 2019, © 2019 Taylor & Francis Group, LLC.
T2  - International Journal of Radiation Biology
T1  - Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers
VL  - 95
IS  - 3
SP  - 274
EP  - 285
DO  - 10.1080/09553002.2019.1549753
ER  - 

@article{
author = "Petković, Vladana and Keta, Otilija D. and Vidosavljević, Marija Z. and Incerti, Sebastien and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2019",
abstract = "Purpose: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high linear energy transfer (LET) radiations used in hadron therapy, i.e. protons and carbon ions. Materials and methods: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers. Biological response to irradiation is evaluated through clonogenic cell survival, immunocytochemical and cell cycle analysis, as well as expression of proteins involved in DNA damage response. Results: Examined cell lines reveal similar level of radioresistance. Application of scavengers leads to the rise of cell survival and decreases the number of DNA double strand breaks in irradiated cells. Differences in cell cycle and protein expression between the two cell lines are probably caused by different DNA damage repair mechanisms that are activated. Conclusion: The obtained results show that DMSO and glycerol have good scavenging capacity, and may be used to minimize DNA damage induced by free radicals. Therefore, they will be used as the reference for comparison with high LET irradiations, as well as good experimental data suitable for validation of numerical simulations. © 2019, © 2019 Taylor & Francis Group, LLC.",
journal = "International Journal of Radiation Biology",
title = "Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers",
volume = "95",
number = "3",
pages = "274-285",
doi = "10.1080/09553002.2019.1549753"
}

Petković, V., Keta, O. D., Vidosavljević, M. Z., Incerti, S., Ristić-Fira, A.,& Petrović, I. M.. (2019). Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers. in International Journal of Radiation Biology, 95(3), 274-285.
https://doi.org/10.1080/09553002.2019.1549753

Petković V, Keta OD, Vidosavljević MZ, Incerti S, Ristić-Fira A, Petrović IM. Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers. in International Journal of Radiation Biology. 2019;95(3):274-285.
doi:10.1080/09553002.2019.1549753 .

Petković, Vladana, Keta, Otilija D., Vidosavljević, Marija Z., Incerti, Sebastien, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers" in International Journal of Radiation Biology, 95, no. 3 (2019):274-285,
https://doi.org/10.1080/09553002.2019.1549753 . .

TY  - JOUR
AU  - Sakata, Dousatsu
AU  - Lampe, Nathanael
AU  - Karamitros, Mathieu
AU  - Kyriakou, Ioanna
AU  - Belov, Oleg
AU  - Bernal, Mario A
AU  - Bolst, David
AU  - Bordage, Marie-Claude
AU  - Breton, Vincent
AU  - Brown, Jeremy M.C.
AU  - Francis, Ziad
AU  - Ivanchenko, Vladimir
AU  - Meylan, Sylvain
AU  - Murakami, Koichi
AU  - Okada, Shogo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Santin, Giovanni
AU  - Sarramia, David
AU  - Sasaki, Takashi
AU  - Shin, Wook-Geun
AU  - Tang, Nicolas
AU  - Tran, Hoang N
AU  - Villagrasa, Carmen
AU  - Emfietzoglou, Dimitris
AU  - Nieminen, Petteri
AU  - Guatelli, Susanna
AU  - Incerti, Sebastien
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1120179719300882
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8206
AB  - The advancement of multidisciplinary research fields dealing with ionising radiation induced biological damage – radiobiology, radiation physics, radiation protection and, in particular, medical physics – requires a clear mechanistic understanding of how cellular damage is induced by ionising radiation. Monte Carlo (MC)simulations provide a promising approach for the mechanistic simulation of radiation transport and radiation chemistry, towards the in silico simulation of early biological damage. We have recently developed a fully integrated MC simulation that calculates early single strand breaks (SSBs)and double strand breaks (DSBs)in a fractal chromatin based human cell nucleus model. The results of this simulation are almost equivalent to past MC simulations when considering direct/indirect strand break fraction, DSB yields and fragment distribution. The simulation results agree with experimental data on DSB yields within 13.6% on average and fragment distributions agree within an average of 34.8%. © 2019 Associazione Italiana di Fisica Medica
T2  - Physica Medica
T1  - Evaluation of early radiation DNA damage in a fractal cell nucleus model using Geant4-DNA
VL  - 62
SP  - 152
EP  - 157
DO  - 10.1016/j.ejmp.2019.04.010
ER  - 

@article{
author = "Sakata, Dousatsu and Lampe, Nathanael and Karamitros, Mathieu and Kyriakou, Ioanna and Belov, Oleg and Bernal, Mario A and Bolst, David and Bordage, Marie-Claude and Breton, Vincent and Brown, Jeremy M.C. and Francis, Ziad and Ivanchenko, Vladimir and Meylan, Sylvain and Murakami, Koichi and Okada, Shogo and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Santin, Giovanni and Sarramia, David and Sasaki, Takashi and Shin, Wook-Geun and Tang, Nicolas and Tran, Hoang N and Villagrasa, Carmen and Emfietzoglou, Dimitris and Nieminen, Petteri and Guatelli, Susanna and Incerti, Sebastien",
year = "2019",
abstract = "The advancement of multidisciplinary research fields dealing with ionising radiation induced biological damage – radiobiology, radiation physics, radiation protection and, in particular, medical physics – requires a clear mechanistic understanding of how cellular damage is induced by ionising radiation. Monte Carlo (MC)simulations provide a promising approach for the mechanistic simulation of radiation transport and radiation chemistry, towards the in silico simulation of early biological damage. We have recently developed a fully integrated MC simulation that calculates early single strand breaks (SSBs)and double strand breaks (DSBs)in a fractal chromatin based human cell nucleus model. The results of this simulation are almost equivalent to past MC simulations when considering direct/indirect strand break fraction, DSB yields and fragment distribution. The simulation results agree with experimental data on DSB yields within 13.6% on average and fragment distributions agree within an average of 34.8%. © 2019 Associazione Italiana di Fisica Medica",
journal = "Physica Medica",
title = "Evaluation of early radiation DNA damage in a fractal cell nucleus model using Geant4-DNA",
volume = "62",
pages = "152-157",
doi = "10.1016/j.ejmp.2019.04.010"
}

Sakata, D., Lampe, N., Karamitros, M., Kyriakou, I., Belov, O., Bernal, M. A., Bolst, D., Bordage, M., Breton, V., Brown, J. M.C., Francis, Z., Ivanchenko, V., Meylan, S., Murakami, K., Okada, S., Petrović, I. M., Ristić-Fira, A., Santin, G., Sarramia, D., Sasaki, T., Shin, W., Tang, N., Tran, H. N., Villagrasa, C., Emfietzoglou, D., Nieminen, P., Guatelli, S.,& Incerti, S.. (2019). Evaluation of early radiation DNA damage in a fractal cell nucleus model using Geant4-DNA. in Physica Medica, 62, 152-157.
https://doi.org/10.1016/j.ejmp.2019.04.010

Sakata D, Lampe N, Karamitros M, Kyriakou I, Belov O, Bernal MA, Bolst D, Bordage M, Breton V, Brown JM, Francis Z, Ivanchenko V, Meylan S, Murakami K, Okada S, Petrović IM, Ristić-Fira A, Santin G, Sarramia D, Sasaki T, Shin W, Tang N, Tran HN, Villagrasa C, Emfietzoglou D, Nieminen P, Guatelli S, Incerti S. Evaluation of early radiation DNA damage in a fractal cell nucleus model using Geant4-DNA. in Physica Medica. 2019;62:152-157.
doi:10.1016/j.ejmp.2019.04.010 .

Sakata, Dousatsu, Lampe, Nathanael, Karamitros, Mathieu, Kyriakou, Ioanna, Belov, Oleg, Bernal, Mario A, Bolst, David, Bordage, Marie-Claude, Breton, Vincent, Brown, Jeremy M.C., Francis, Ziad, Ivanchenko, Vladimir, Meylan, Sylvain, Murakami, Koichi, Okada, Shogo, Petrović, Ivan M., Ristić-Fira, Aleksandra, Santin, Giovanni, Sarramia, David, Sasaki, Takashi, Shin, Wook-Geun, Tang, Nicolas, Tran, Hoang N, Villagrasa, Carmen, Emfietzoglou, Dimitris, Nieminen, Petteri, Guatelli, Susanna, Incerti, Sebastien, "Evaluation of early radiation DNA damage in a fractal cell nucleus model using Geant4-DNA" in Physica Medica, 62 (2019):152-157,
https://doi.org/10.1016/j.ejmp.2019.04.010 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Bulat, Tanja M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1573
AB  - The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied.
T2  - Experimental Biology and Medicine
T1  - Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions
VL  - 242
IS  - 10
SP  - 1015
EP  - 1024
DO  - 10.1177/1535370216669611
ER  - 

@article{
author = "Keta, Otilija D. and Todorović, Danijela V. and Bulat, Tanja M. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2017",
abstract = "The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied.",
journal = "Experimental Biology and Medicine",
title = "Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions",
volume = "242",
number = "10",
pages = "1015-1024",
doi = "10.1177/1535370216669611"
}

Keta, O. D., Todorović, D. V., Bulat, T. M., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2017). Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. in Experimental Biology and Medicine, 242(10), 1015-1024.
https://doi.org/10.1177/1535370216669611

Keta OD, Todorović DV, Bulat TM, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. in Experimental Biology and Medicine. 2017;242(10):1015-1024.
doi:10.1177/1535370216669611 .

Keta, Otilija D., Todorović, Danijela V., Bulat, Tanja M., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions" in Experimental Biology and Medicine, 242, no. 10 (2017):1015-1024,
https://doi.org/10.1177/1535370216669611 . .

TY  - JOUR
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1290
AB  - Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.
T2  - Indian Journal of Medical Research
T1  - Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells
VL  - 143
SP  - 120
EP  - 128
DO  - 10.4103/0971-5916.191811
ER  - 

@article{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Todorović, Danijela V. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.",
journal = "Indian Journal of Medical Research",
title = "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells",
volume = "143",
pages = "120-128",
doi = "10.4103/0971-5916.191811"
}

Žakula, J., Korićanac, L., Keta, O. D., Todorović, D. V., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2016). Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research, 143, 120-128.
https://doi.org/10.4103/0971-5916.191811

Žakula J, Korićanac L, Keta OD, Todorović DV, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research. 2016;143:120-128.
doi:10.4103/0971-5916.191811 .

Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Todorović, Danijela V., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells" in Indian Journal of Medical Research, 143 (2016):120-128,
https://doi.org/10.4103/0971-5916.191811 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Golic, Igor
AU  - Incerti, Sebastien
AU  - Korać, Aleksandra
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1044
AB  - In most patients with lung cancer radiation treatment is used either as single agent or in combination with radiosensitizing drugs. However, the mechanisms underlying combined therapy and its impact on different modes of cell death have not yet been fully elucidated. We aimed to examine effects of single and combined treatments with gamma-rays and erlotinib on radioresistant CRL-5876 human lung adenocarcinoma cells with particular emphasis on cell death. CRL-5876 cells were treated with gamma-rays and/or erlotinib and changes in cell cycle, DNA repair dynamics, ultrastructure, nuclear morphology and protein expression were monitored at different time points. To reveal the relationship between types of cell death that arise after these treatments, autophagy was blocked with chloroquine. We found that higher dose of gamma-rays causes G2/M arrest while adding of erlotinib to this treatment decreases the number of cells in S phase. Impact of erlotinib on kinetics of disappearance of irradiation-induced DNA double strand breaks is reflected in the increase of residual gamma-H2AX foci after 24 h. gamma-rays provoke cytoprotective autophagy which precedes development of senescence. Erlotinib predominantly induces apoptosis and enlarges the number of apoptotic cells in the irradiated CRL-5876 cells. Chloroquine improved cytotoxicity induced by radiation and erlotinib, increased apoptosis and decreased senescence in the CRL-5876 cells. The results obtained on CRL-5876 cells indicate significant radiosensitizing effect of erlotinib and suggest that chloroquine in the combination with the above treatments may have an additional antitumor effect in lung adenocarcinoma.
PB  - Springer
T2  - Cell Biology and Toxicology
T1  - The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib
VL  - 32
IS  - 2
SP  - 83
EP  - 101
DO  - 10.1007/s10565-016-9319-z
ER  - 

@article{
author = "Keta, Otilija D. and Bulat, Tanja M. and Golic, Igor and Incerti, Sebastien and Korać, Aleksandra and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "In most patients with lung cancer radiation treatment is used either as single agent or in combination with radiosensitizing drugs. However, the mechanisms underlying combined therapy and its impact on different modes of cell death have not yet been fully elucidated. We aimed to examine effects of single and combined treatments with gamma-rays and erlotinib on radioresistant CRL-5876 human lung adenocarcinoma cells with particular emphasis on cell death. CRL-5876 cells were treated with gamma-rays and/or erlotinib and changes in cell cycle, DNA repair dynamics, ultrastructure, nuclear morphology and protein expression were monitored at different time points. To reveal the relationship between types of cell death that arise after these treatments, autophagy was blocked with chloroquine. We found that higher dose of gamma-rays causes G2/M arrest while adding of erlotinib to this treatment decreases the number of cells in S phase. Impact of erlotinib on kinetics of disappearance of irradiation-induced DNA double strand breaks is reflected in the increase of residual gamma-H2AX foci after 24 h. gamma-rays provoke cytoprotective autophagy which precedes development of senescence. Erlotinib predominantly induces apoptosis and enlarges the number of apoptotic cells in the irradiated CRL-5876 cells. Chloroquine improved cytotoxicity induced by radiation and erlotinib, increased apoptosis and decreased senescence in the CRL-5876 cells. The results obtained on CRL-5876 cells indicate significant radiosensitizing effect of erlotinib and suggest that chloroquine in the combination with the above treatments may have an additional antitumor effect in lung adenocarcinoma.",
publisher = "Springer",
journal = "Cell Biology and Toxicology",
title = "The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib",
volume = "32",
number = "2",
pages = "83-101",
doi = "10.1007/s10565-016-9319-z"
}

Keta, O. D., Bulat, T. M., Golic, I., Incerti, S., Korać, A., Petrović, I. M.,& Ristić-Fira, A.. (2016). The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib. in Cell Biology and Toxicology
Springer., 32(2), 83-101.
https://doi.org/10.1007/s10565-016-9319-z

Keta OD, Bulat TM, Golic I, Incerti S, Korać A, Petrović IM, Ristić-Fira A. The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib. in Cell Biology and Toxicology. 2016;32(2):83-101.
doi:10.1007/s10565-016-9319-z .

Keta, Otilija D., Bulat, Tanja M., Golic, Igor, Incerti, Sebastien, Korać, Aleksandra, Petrović, Ivan M., Ristić-Fira, Aleksandra, "The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib" in Cell Biology and Toxicology, 32, no. 2 (2016):83-101,
https://doi.org/10.1007/s10565-016-9319-z . .

TY  - JOUR
AU  - Bulat, Tanja M.
AU  - Keta, Otilija D.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/970
AB  - Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (gamma H2AX). Immunofluorescent staining visualizes formation of gamma H2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of gamma H2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to gamma-rays, in the dose range from 1 to 16 Gy. Radiation effects on the DNA level were analyzed at different time intervals after irradiation by Western blot analysis and immunofluorescence microscopy. Immunochemically stained cells were visualized with two types of microscopes: AxioVision (Zeiss, Germany) microscope, comprising an ApoTome software, and AxioImagerA1 microscope (Zeiss, Germany). Obtained results show that the level of gamma H2AX is time and dose dependent. Immunofluorescence microscopy provided better detection of DSBs for lower irradiation doses, while Western blot analysis was more reliable for higher irradiation doses. AxioVision microscope containing ApoTome software was more suitable for the detection of gamma H2AX foci.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Radiation dose determines the method for quantification of DNA double strand breaks
VL  - 88
IS  - 1
SP  - 127
EP  - 136
DO  - 10.1590/0001-3765201620140553
ER  - 

@article{
author = "Bulat, Tanja M. and Keta, Otilija D. and Korićanac, Lela and Žakula, Jelena and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V.",
year = "2016",
abstract = "Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (gamma H2AX). Immunofluorescent staining visualizes formation of gamma H2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of gamma H2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to gamma-rays, in the dose range from 1 to 16 Gy. Radiation effects on the DNA level were analyzed at different time intervals after irradiation by Western blot analysis and immunofluorescence microscopy. Immunochemically stained cells were visualized with two types of microscopes: AxioVision (Zeiss, Germany) microscope, comprising an ApoTome software, and AxioImagerA1 microscope (Zeiss, Germany). Obtained results show that the level of gamma H2AX is time and dose dependent. Immunofluorescence microscopy provided better detection of DSBs for lower irradiation doses, while Western blot analysis was more reliable for higher irradiation doses. AxioVision microscope containing ApoTome software was more suitable for the detection of gamma H2AX foci.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Radiation dose determines the method for quantification of DNA double strand breaks",
volume = "88",
number = "1",
pages = "127-136",
doi = "10.1590/0001-3765201620140553"
}

Bulat, T. M., Keta, O. D., Korićanac, L., Žakula, J., Petrović, I. M., Ristić-Fira, A.,& Todorović, D. V.. (2016). Radiation dose determines the method for quantification of DNA double strand breaks. in Anais de Academia Brasileira de Ciencias, 88(1), 127-136.
https://doi.org/10.1590/0001-3765201620140553

Bulat TM, Keta OD, Korićanac L, Žakula J, Petrović IM, Ristić-Fira A, Todorović DV. Radiation dose determines the method for quantification of DNA double strand breaks. in Anais de Academia Brasileira de Ciencias. 2016;88(1):127-136.
doi:10.1590/0001-3765201620140553 .

Bulat, Tanja M., Keta, Otilija D., Korićanac, Lela, Žakula, Jelena, Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., "Radiation dose determines the method for quantification of DNA double strand breaks" in Anais de Academia Brasileira de Ciencias, 88, no. 1 (2016):127-136,
https://doi.org/10.1590/0001-3765201620140553 . .

TY  - JOUR
AU  - Allison, J
AU  - Amako, K
AU  - Apostolakis, J
AU  - Arce, P
AU  - Asai, M
AU  - Aso, T
AU  - Bagli, E
AU  - Bagulya, A
AU  - Banerjee, S
AU  - Barrand, G
AU  - Beck, B.R.
AU  - Bogdanov, A.G.
AU  - Brandt, D
AU  - Brown, J.M.C.
AU  - Burkhardt, H
AU  - Canal, Ph.
AU  - Cano-Ott, D
AU  - Chauvie, S
AU  - Cho, K
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cooperman, G
AU  - Cortés-Giraldo, M.A.
AU  - Cosmo, G
AU  - Cuttone, Giacomo
AU  - Depaola, G
AU  - Desorgher, L
AU  - Dong, X
AU  - Dotti, A
AU  - Elvira, V.D.
AU  - Folger, G
AU  - Francis, Z
AU  - Galoyan, A
AU  - Garnier, L
AU  - Gayer, M
AU  - Genser, K.L.
AU  - Grichine, V.M.
AU  - Guatelli, S
AU  - Guèye, P
AU  - Gumplinger, P
AU  - Howard, A.S.
AU  - Hřivnáčová, I
AU  - Hwang, S
AU  - Incerti, S
AU  - Ivanchenko, A.
AU  - Ivanchenko, V.N.
AU  - Jones, F.W.
AU  - Jun, S.Y.
AU  - Kaitaniemi, P
AU  - Karakatsanis, N
AU  - Karamitros, M
AU  - Kelsey, M
AU  - Kimura, A
AU  - Koi, T
AU  - Kurashige, H
AU  - Lechner, A
AU  - Lee, S.B.
AU  - Longo, F
AU  - Maire, M
AU  - Mancusi, D
AU  - Mantero, A
AU  - Mendoza, E
AU  - Morgan, B
AU  - Murakami, K
AU  - Nikitina, T
AU  - Pandola, L
AU  - Paprocki, P
AU  - Perl, J
AU  - Petrović, Ivan M.
AU  - Pia, M.G.
AU  - Pokorski, W
AU  - Quesada, J.M.
AU  - Raine, M
AU  - Reis, M.A.
AU  - Ribon, A
AU  - Ristić-Fira, Aleksandra
AU  - Romano, Francesco
AU  - Russo, G
AU  - Santin, G
AU  - Sasaki, T
AU  - Sawkey, D
AU  - Shin, J.I.
AU  - Strakovsky, I.I.
AU  - Taborda, A
AU  - Tanaka, S
AU  - Tomé, B
AU  - Toshito, T
AU  - Tran, H.N.
AU  - Truscott, P.R.
AU  - Urban, L
AU  - Uzhinsky, V
AU  - Verbeke, J.M.
AU  - Verderi, M
AU  - Wendt, B.L.
AU  - Wenzel, H
AU  - Wright, D.H.
AU  - Wright, D.M.
AU  - Yamashita, T
AU  - Yarba, J
AU  - Yoshida, H
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8645
AB  - Geant4 is a software toolkit for the simulation of the passage of particles through matter. It is used by a large number of experiments and projects in a variety of application domains, including high energy physics, astrophysics and space science, medical physics and radiation protection. Over the past several years, major changes have been made to the toolkit in order to accommodate the needs of these user communities, and to efficiently exploit the growth of computing power made available by advances in technology. The adaptation of Geant4 to multithreading, advances in physics, detector modeling and visualization, extensions to the toolkit, including biasing and reverse Monte Carlo, and tools for physics and release validation are discussed here.
T2  - Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
T1  - Recent developments in Geant4
VL  - 835
SP  - 186
EP  - 225
DO  - 10.1016/j.nima.2016.06.125
ER  - 

@article{
author = "Allison, J and Amako, K and Apostolakis, J and Arce, P and Asai, M and Aso, T and Bagli, E and Bagulya, A and Banerjee, S and Barrand, G and Beck, B.R. and Bogdanov, A.G. and Brandt, D and Brown, J.M.C. and Burkhardt, H and Canal, Ph. and Cano-Ott, D and Chauvie, S and Cho, K and Cirrone, Giuseppe Antonio Pablo and Cooperman, G and Cortés-Giraldo, M.A. and Cosmo, G and Cuttone, Giacomo and Depaola, G and Desorgher, L and Dong, X and Dotti, A and Elvira, V.D. and Folger, G and Francis, Z and Galoyan, A and Garnier, L and Gayer, M and Genser, K.L. and Grichine, V.M. and Guatelli, S and Guèye, P and Gumplinger, P and Howard, A.S. and Hřivnáčová, I and Hwang, S and Incerti, S and Ivanchenko, A. and Ivanchenko, V.N. and Jones, F.W. and Jun, S.Y. and Kaitaniemi, P and Karakatsanis, N and Karamitros, M and Kelsey, M and Kimura, A and Koi, T and Kurashige, H and Lechner, A and Lee, S.B. and Longo, F and Maire, M and Mancusi, D and Mantero, A and Mendoza, E and Morgan, B and Murakami, K and Nikitina, T and Pandola, L and Paprocki, P and Perl, J and Petrović, Ivan M. and Pia, M.G. and Pokorski, W and Quesada, J.M. and Raine, M and Reis, M.A. and Ribon, A and Ristić-Fira, Aleksandra and Romano, Francesco and Russo, G and Santin, G and Sasaki, T and Sawkey, D and Shin, J.I. and Strakovsky, I.I. and Taborda, A and Tanaka, S and Tomé, B and Toshito, T and Tran, H.N. and Truscott, P.R. and Urban, L and Uzhinsky, V and Verbeke, J.M. and Verderi, M and Wendt, B.L. and Wenzel, H and Wright, D.H. and Wright, D.M. and Yamashita, T and Yarba, J and Yoshida, H",
year = "2016",
abstract = "Geant4 is a software toolkit for the simulation of the passage of particles through matter. It is used by a large number of experiments and projects in a variety of application domains, including high energy physics, astrophysics and space science, medical physics and radiation protection. Over the past several years, major changes have been made to the toolkit in order to accommodate the needs of these user communities, and to efficiently exploit the growth of computing power made available by advances in technology. The adaptation of Geant4 to multithreading, advances in physics, detector modeling and visualization, extensions to the toolkit, including biasing and reverse Monte Carlo, and tools for physics and release validation are discussed here.",
journal = "Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment",
title = "Recent developments in Geant4",
volume = "835",
pages = "186-225",
doi = "10.1016/j.nima.2016.06.125"
}

Allison, J., Amako, K., Apostolakis, J., Arce, P., Asai, M., Aso, T., Bagli, E., Bagulya, A., Banerjee, S., Barrand, G., Beck, B.R., Bogdanov, A.G., Brandt, D., Brown, J.M.C., Burkhardt, H., Canal, Ph., Cano-Ott, D., Chauvie, S., Cho, K., Cirrone, G. A. P., Cooperman, G., Cortés-Giraldo, M.A., Cosmo, G., Cuttone, G., Depaola, G., Desorgher, L., Dong, X., Dotti, A., Elvira, V.D., Folger, G., Francis, Z., Galoyan, A., Garnier, L., Gayer, M., Genser, K.L., Grichine, V.M., Guatelli, S., Guèye, P., Gumplinger, P., Howard, A.S., Hřivnáčová, I., Hwang, S., Incerti, S., Ivanchenko, A., Ivanchenko, V.N., Jones, F.W., Jun, S.Y., Kaitaniemi, P., Karakatsanis, N., Karamitros, M., Kelsey, M., Kimura, A., Koi, T., Kurashige, H., Lechner, A., Lee, S.B., Longo, F., Maire, M., Mancusi, D., Mantero, A., Mendoza, E., Morgan, B., Murakami, K., Nikitina, T., Pandola, L., Paprocki, P., Perl, J., Petrović, I. M., Pia, M.G., Pokorski, W., Quesada, J.M., Raine, M., Reis, M.A., Ribon, A., Ristić-Fira, A., Romano, F., Russo, G., Santin, G., Sasaki, T., Sawkey, D., Shin, J.I., Strakovsky, I.I., Taborda, A., Tanaka, S., Tomé, B., Toshito, T., Tran, H.N., Truscott, P.R., Urban, L., Uzhinsky, V., Verbeke, J.M., Verderi, M., Wendt, B.L., Wenzel, H., Wright, D.H., Wright, D.M., Yamashita, T., Yarba, J.,& Yoshida, H.. (2016). Recent developments in Geant4. in Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 835, 186-225.
https://doi.org/10.1016/j.nima.2016.06.125

Allison J, Amako K, Apostolakis J, Arce P, Asai M, Aso T, Bagli E, Bagulya A, Banerjee S, Barrand G, Beck B, Bogdanov A, Brandt D, Brown J, Burkhardt H, Canal P, Cano-Ott D, Chauvie S, Cho K, Cirrone GAP, Cooperman G, Cortés-Giraldo M, Cosmo G, Cuttone G, Depaola G, Desorgher L, Dong X, Dotti A, Elvira V, Folger G, Francis Z, Galoyan A, Garnier L, Gayer M, Genser K, Grichine V, Guatelli S, Guèye P, Gumplinger P, Howard A, Hřivnáčová I, Hwang S, Incerti S, Ivanchenko A, Ivanchenko V, Jones F, Jun S, Kaitaniemi P, Karakatsanis N, Karamitros M, Kelsey M, Kimura A, Koi T, Kurashige H, Lechner A, Lee S, Longo F, Maire M, Mancusi D, Mantero A, Mendoza E, Morgan B, Murakami K, Nikitina T, Pandola L, Paprocki P, Perl J, Petrović IM, Pia M, Pokorski W, Quesada J, Raine M, Reis M, Ribon A, Ristić-Fira A, Romano F, Russo G, Santin G, Sasaki T, Sawkey D, Shin J, Strakovsky I, Taborda A, Tanaka S, Tomé B, Toshito T, Tran H, Truscott P, Urban L, Uzhinsky V, Verbeke J, Verderi M, Wendt B, Wenzel H, Wright D, Wright D, Yamashita T, Yarba J, Yoshida H. Recent developments in Geant4. in Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. 2016;835:186-225.
doi:10.1016/j.nima.2016.06.125 .

Allison, J, Amako, K, Apostolakis, J, Arce, P, Asai, M, Aso, T, Bagli, E, Bagulya, A, Banerjee, S, Barrand, G, Beck, B.R., Bogdanov, A.G., Brandt, D, Brown, J.M.C., Burkhardt, H, Canal, Ph., Cano-Ott, D, Chauvie, S, Cho, K, Cirrone, Giuseppe Antonio Pablo, Cooperman, G, Cortés-Giraldo, M.A., Cosmo, G, Cuttone, Giacomo, Depaola, G, Desorgher, L, Dong, X, Dotti, A, Elvira, V.D., Folger, G, Francis, Z, Galoyan, A, Garnier, L, Gayer, M, Genser, K.L., Grichine, V.M., Guatelli, S, Guèye, P, Gumplinger, P, Howard, A.S., Hřivnáčová, I, Hwang, S, Incerti, S, Ivanchenko, A., Ivanchenko, V.N., Jones, F.W., Jun, S.Y., Kaitaniemi, P, Karakatsanis, N, Karamitros, M, Kelsey, M, Kimura, A, Koi, T, Kurashige, H, Lechner, A, Lee, S.B., Longo, F, Maire, M, Mancusi, D, Mantero, A, Mendoza, E, Morgan, B, Murakami, K, Nikitina, T, Pandola, L, Paprocki, P, Perl, J, Petrović, Ivan M., Pia, M.G., Pokorski, W, Quesada, J.M., Raine, M, Reis, M.A., Ribon, A, Ristić-Fira, Aleksandra, Romano, Francesco, Russo, G, Santin, G, Sasaki, T, Sawkey, D, Shin, J.I., Strakovsky, I.I., Taborda, A, Tanaka, S, Tomé, B, Toshito, T, Tran, H.N., Truscott, P.R., Urban, L, Uzhinsky, V, Verbeke, J.M., Verderi, M, Wendt, B.L., Wenzel, H, Wright, D.H., Wright, D.M., Yamashita, T, Yarba, J, Yoshida, H, "Recent developments in Geant4" in Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 835 (2016):186-225,
https://doi.org/10.1016/j.nima.2016.06.125 . .

TY  - CONF
AU  - Petković, Vladana
AU  - Keta, Otilija D.
AU  - Vojinović, N.
AU  - Incerti, S.
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9207
AB  - Direct  effects  of  radiation  affect  the  DNA  molecule,  causing  DNAdamage and finally  cell death. We examined the role of DMSO and glycerol as free-radical scavengers in HTB177 cells irradiated with gamma rays. Direct effects of   radiation   were   estimated   through   DNA   double   strand   break   (DSB) quantification  and  cell  survival.  Results  of  this  work  revealed  that  chosen concentration   of   DMSO   exhibit   higher   protective   effect   comparing   to glycerol.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
T1  - Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays
SP  - 447
EP  - 450
ER  - 

@conference{
author = "Petković, Vladana and Keta, Otilija D. and Vojinović, N. and Incerti, S. and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "Direct  effects  of  radiation  affect  the  DNA  molecule,  causing  DNAdamage and finally  cell death. We examined the role of DMSO and glycerol as free-radical scavengers in HTB177 cells irradiated with gamma rays. Direct effects of   radiation   were   estimated   through   DNA   double   strand   break   (DSB) quantification  and  cell  survival.  Results  of  this  work  revealed  that  chosen concentration   of   DMSO   exhibit   higher   protective   effect   comparing   to glycerol.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry",
title = "Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays",
pages = "447-450"
}

Petković, V., Keta, O. D., Vojinović, N., Incerti, S., Petrović, I. M.,& Ristić-Fira, A.. (2016). Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 447-450.

Petković V, Keta OD, Vojinović N, Incerti S, Petrović IM, Ristić-Fira A. Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry. 2016;:447-450..

Petković, Vladana, Keta, Otilija D., Vojinović, N., Incerti, S., Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays" in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry (2016):447-450.

TY  - JOUR
AU  - Bernal, Mario A.
AU  - Bordage, Marie Claude
AU  - Brown, Jeremy Michael Cooney
AU  - Davídková, Marie
AU  - Delage, E.
AU  - El Bitar, Ziad Ei
AU  - Enger, Shirin A.
AU  - Francis, Ziad
AU  - Guatelli, Susanna
AU  - Ivanchenko, Vladimir N.
AU  - Karamitros, Mathieu
AU  - Kyriakou, Ioanna
AU  - Maigne, Lydia
AU  - Meylan, S.
AU  - Murakami, Kouichi
AU  - Okada, Shogo
AU  - Payno, H.
AU  - Perrot, Yann
AU  - Petrović, Ivan M.
AU  - Pham, Q. T.
AU  - Ristić-Fira, Aleksandra
AU  - Sasaki, Takashi
AU  - Stepan, Vaclav
AU  - Tran, Hoang Ngoc
AU  - Villagrasa, Carmen
AU  - Incerti, Sebastien
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/859
AB  - Understanding the fundamental mechanisms involved in the induction of biological damage by ionizing radiation remains a major challenge of todays radiobiology research. The Monte Carlo simulation of physical, physicochemical and chemical processes involved may provide a powerful tool for the simulation of early damage induction. The Geant4-DNA extension of the general purpose Monte Carlo Geant4 simulation toolkit aims to provide the scientific community with an open source access platform for the mechanistic simulation of such early damage. This paper presents the most recent review of the Geant4-DNA extension, as available to Geant4 users since June 2015 (release 10.2 Beta). In particular, the review includes the description of new physical models for the description of electron elastic and inelastic interactions in liquid water, as well as new examples dedicated to the simulation of physicochemical and chemical stages of water radiolysis. Several implementations of geometrical models of biological targets are presented as well, and the list of Geant4-DNA examples is described. (C) 2015 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.
PB  - Elsevier
T2  - Physica Medica -European Journal of Medical Physics
T1  - Track structure modeling in liquid water: A review of the Geant4-DNA very low energy extension of the Geant4 Monte Carlo simulation toolkit
VL  - 31
IS  - 8
SP  - 861
EP  - 874
DO  - 10.1016/j.ejmp.2015.10.087
ER  - 

@article{
author = "Bernal, Mario A. and Bordage, Marie Claude and Brown, Jeremy Michael Cooney and Davídková, Marie and Delage, E. and El Bitar, Ziad Ei and Enger, Shirin A. and Francis, Ziad and Guatelli, Susanna and Ivanchenko, Vladimir N. and Karamitros, Mathieu and Kyriakou, Ioanna and Maigne, Lydia and Meylan, S. and Murakami, Kouichi and Okada, Shogo and Payno, H. and Perrot, Yann and Petrović, Ivan M. and Pham, Q. T. and Ristić-Fira, Aleksandra and Sasaki, Takashi and Stepan, Vaclav and Tran, Hoang Ngoc and Villagrasa, Carmen and Incerti, Sebastien",
year = "2015",
abstract = "Understanding the fundamental mechanisms involved in the induction of biological damage by ionizing radiation remains a major challenge of todays radiobiology research. The Monte Carlo simulation of physical, physicochemical and chemical processes involved may provide a powerful tool for the simulation of early damage induction. The Geant4-DNA extension of the general purpose Monte Carlo Geant4 simulation toolkit aims to provide the scientific community with an open source access platform for the mechanistic simulation of such early damage. This paper presents the most recent review of the Geant4-DNA extension, as available to Geant4 users since June 2015 (release 10.2 Beta). In particular, the review includes the description of new physical models for the description of electron elastic and inelastic interactions in liquid water, as well as new examples dedicated to the simulation of physicochemical and chemical stages of water radiolysis. Several implementations of geometrical models of biological targets are presented as well, and the list of Geant4-DNA examples is described. (C) 2015 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Physica Medica -European Journal of Medical Physics",
title = "Track structure modeling in liquid water: A review of the Geant4-DNA very low energy extension of the Geant4 Monte Carlo simulation toolkit",
volume = "31",
number = "8",
pages = "861-874",
doi = "10.1016/j.ejmp.2015.10.087"
}

Bernal, M. A., Bordage, M. C., Brown, J. M. C., Davídková, M., Delage, E., El Bitar, Z. E., Enger, S. A., Francis, Z., Guatelli, S., Ivanchenko, V. N., Karamitros, M., Kyriakou, I., Maigne, L., Meylan, S., Murakami, K., Okada, S., Payno, H., Perrot, Y., Petrović, I. M., Pham, Q. T., Ristić-Fira, A., Sasaki, T., Stepan, V., Tran, H. N., Villagrasa, C.,& Incerti, S.. (2015). Track structure modeling in liquid water: A review of the Geant4-DNA very low energy extension of the Geant4 Monte Carlo simulation toolkit. in Physica Medica -European Journal of Medical Physics
Elsevier., 31(8), 861-874.
https://doi.org/10.1016/j.ejmp.2015.10.087

Bernal MA, Bordage MC, Brown JMC, Davídková M, Delage E, El Bitar ZE, Enger SA, Francis Z, Guatelli S, Ivanchenko VN, Karamitros M, Kyriakou I, Maigne L, Meylan S, Murakami K, Okada S, Payno H, Perrot Y, Petrović IM, Pham QT, Ristić-Fira A, Sasaki T, Stepan V, Tran HN, Villagrasa C, Incerti S. Track structure modeling in liquid water: A review of the Geant4-DNA very low energy extension of the Geant4 Monte Carlo simulation toolkit. in Physica Medica -European Journal of Medical Physics. 2015;31(8):861-874.
doi:10.1016/j.ejmp.2015.10.087 .

Bernal, Mario A., Bordage, Marie Claude, Brown, Jeremy Michael Cooney, Davídková, Marie, Delage, E., El Bitar, Ziad Ei, Enger, Shirin A., Francis, Ziad, Guatelli, Susanna, Ivanchenko, Vladimir N., Karamitros, Mathieu, Kyriakou, Ioanna, Maigne, Lydia, Meylan, S., Murakami, Kouichi, Okada, Shogo, Payno, H., Perrot, Yann, Petrović, Ivan M., Pham, Q. T., Ristić-Fira, Aleksandra, Sasaki, Takashi, Stepan, Vaclav, Tran, Hoang Ngoc, Villagrasa, Carmen, Incerti, Sebastien, "Track structure modeling in liquid water: A review of the Geant4-DNA very low energy extension of the Geant4 Monte Carlo simulation toolkit" in Physica Medica -European Journal of Medical Physics, 31, no. 8 (2015):861-874,
https://doi.org/10.1016/j.ejmp.2015.10.087 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Popović, Nataša M.
AU  - Korićanac, Lela
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5447
AB  - Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.
T2  - Archives of Medical Science
T1  - Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons
VL  - 10
IS  - 3
SP  - 578
EP  - 586
DO  - 10.5114/aoms.2014.43751
ER  - 

@article{
author = "Keta, Otilija D. and Todorović, Danijela V. and Popović, Nataša M. and Korićanac, Lela and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2014",
abstract = "Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.",
journal = "Archives of Medical Science",
title = "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons",
volume = "10",
number = "3",
pages = "578-586",
doi = "10.5114/aoms.2014.43751"
}

Keta, O. D., Todorović, D. V., Popović, N. M., Korićanac, L., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2014). Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. in Archives of Medical Science, 10(3), 578-586.
https://doi.org/10.5114/aoms.2014.43751

Keta OD, Todorović DV, Popović NM, Korićanac L, Cuttone G, Petrović IM, Ristić-Fira A. Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. in Archives of Medical Science. 2014;10(3):578-586.
doi:10.5114/aoms.2014.43751 .

Keta, Otilija D., Todorović, Danijela V., Popović, Nataša M., Korićanac, Lela, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons" in Archives of Medical Science, 10, no. 3 (2014):578-586,
https://doi.org/10.5114/aoms.2014.43751 . .

TY  - CONF
AU  - Schillaci, F.
AU  - Anzalone, A.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Carpinelli, M.
AU  - Cuttone, Giacomo
AU  - Cutroneo, M.
AU  - De Martinis, C.
AU  - Giove, D.
AU  - Korn, G.
AU  - Maggiore, M.
AU  - Manti, L.
AU  - Margarone, D.
AU  - Musumarra, A.
AU  - Perozziello, F. M.
AU  - Petrović, Ivan M.
AU  - Pisciotta, P.
AU  - Renis, M.
AU  - Ristić-Fira, Aleksandra
AU  - Romano, Francesco
AU  - Romano, Francesco
AU  - Schettino, G.
AU  - Scuderi, V.
AU  - Torrisi, L.
AU  - Tramontana, A.
AU  - Tudisco, S.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7029
AB  - ELI-Beamlines is one of the pillars of the pan-European project ELI (Extreme Light Infrastructure). It will be an ultra high-intensity, high repetition-rate, femtosecond laser facility whose main goal is generation and applications of high-brightness X-ray sources and accelerated charged particles in different fields. Particular care will be devoted to the potential applicability of laser-driven ion beams for medical treatments of tumors. Indeed, such kind of beams show very interesting peculiarities and, moreover, laser-driven based accelerators can really represent a competitive alternative to conventional machines since they are expected to be more compact in size and less expensive. The ELIMED project was launched thanks to a collaboration established between FZU-ASCR (ELI-Beamlines) and INFN-LNS researchers. Several European institutes have already shown a great interest in the project aiming to explore the possibility to use laser-driven ion (mostly proton) beams for several applications with a particular regard for medical ones. To reach the project goal several tasks need to be fulfilled, starting from the optimization of laser-target interaction to dosimetric studies at the irradiation point at the end of a proper designed transport beam-line. Researchers from LNS have already developed and successfully tested a high-dispersive power Thomson Parabola Spectrometer, which is the first prototype of a more performing device to be used within the ELIMED project. Also a Magnetic Selection System able to produce a small pencil beam out of a wide energy distribution of ions produced in laser-target interaction has been realized and some preliminary work for its testing and characterization is in progress. In this contribution the status of the project will be reported together with a short description of the of the features of device recently developed.
C3  - Journal of Physics: Conference Series
T1  - ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines
VL  - 508
DO  - 10.1088/1742-6596/508/1/012010
ER  - 

@conference{
author = "Schillaci, F. and Anzalone, A. and Cirrone, Giuseppe Antonio Pablo and Carpinelli, M. and Cuttone, Giacomo and Cutroneo, M. and De Martinis, C. and Giove, D. and Korn, G. and Maggiore, M. and Manti, L. and Margarone, D. and Musumarra, A. and Perozziello, F. M. and Petrović, Ivan M. and Pisciotta, P. and Renis, M. and Ristić-Fira, Aleksandra and Romano, Francesco and Romano, Francesco and Schettino, G. and Scuderi, V. and Torrisi, L. and Tramontana, A. and Tudisco, S.",
year = "2014",
abstract = "ELI-Beamlines is one of the pillars of the pan-European project ELI (Extreme Light Infrastructure). It will be an ultra high-intensity, high repetition-rate, femtosecond laser facility whose main goal is generation and applications of high-brightness X-ray sources and accelerated charged particles in different fields. Particular care will be devoted to the potential applicability of laser-driven ion beams for medical treatments of tumors. Indeed, such kind of beams show very interesting peculiarities and, moreover, laser-driven based accelerators can really represent a competitive alternative to conventional machines since they are expected to be more compact in size and less expensive. The ELIMED project was launched thanks to a collaboration established between FZU-ASCR (ELI-Beamlines) and INFN-LNS researchers. Several European institutes have already shown a great interest in the project aiming to explore the possibility to use laser-driven ion (mostly proton) beams for several applications with a particular regard for medical ones. To reach the project goal several tasks need to be fulfilled, starting from the optimization of laser-target interaction to dosimetric studies at the irradiation point at the end of a proper designed transport beam-line. Researchers from LNS have already developed and successfully tested a high-dispersive power Thomson Parabola Spectrometer, which is the first prototype of a more performing device to be used within the ELIMED project. Also a Magnetic Selection System able to produce a small pencil beam out of a wide energy distribution of ions produced in laser-target interaction has been realized and some preliminary work for its testing and characterization is in progress. In this contribution the status of the project will be reported together with a short description of the of the features of device recently developed.",
journal = "Journal of Physics: Conference Series",
title = "ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines",
volume = "508",
doi = "10.1088/1742-6596/508/1/012010"
}

Schillaci, F., Anzalone, A., Cirrone, G. A. P., Carpinelli, M., Cuttone, G., Cutroneo, M., De Martinis, C., Giove, D., Korn, G., Maggiore, M., Manti, L., Margarone, D., Musumarra, A., Perozziello, F. M., Petrović, I. M., Pisciotta, P., Renis, M., Ristić-Fira, A., Romano, F., Romano, F., Schettino, G., Scuderi, V., Torrisi, L., Tramontana, A.,& Tudisco, S.. (2014). ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines. in Journal of Physics: Conference Series, 508.
https://doi.org/10.1088/1742-6596/508/1/012010

Schillaci F, Anzalone A, Cirrone GAP, Carpinelli M, Cuttone G, Cutroneo M, De Martinis C, Giove D, Korn G, Maggiore M, Manti L, Margarone D, Musumarra A, Perozziello FM, Petrović IM, Pisciotta P, Renis M, Ristić-Fira A, Romano F, Romano F, Schettino G, Scuderi V, Torrisi L, Tramontana A, Tudisco S. ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines. in Journal of Physics: Conference Series. 2014;508.
doi:10.1088/1742-6596/508/1/012010 .

Schillaci, F., Anzalone, A., Cirrone, Giuseppe Antonio Pablo, Carpinelli, M., Cuttone, Giacomo, Cutroneo, M., De Martinis, C., Giove, D., Korn, G., Maggiore, M., Manti, L., Margarone, D., Musumarra, A., Perozziello, F. M., Petrović, Ivan M., Pisciotta, P., Renis, M., Ristić-Fira, Aleksandra, Romano, Francesco, Romano, Francesco, Schettino, G., Scuderi, V., Torrisi, L., Tramontana, A., Tudisco, S., "ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines" in Journal of Physics: Conference Series, 508 (2014),
https://doi.org/10.1088/1742-6596/508/1/012010 . .

TY  - JOUR
AU  - Incerti, Sebastien
AU  - Psaltaki, M.
AU  - Gillet, P.
AU  - Barberet, Ph.
AU  - Bardies, M.
AU  - Bernal, Mario A.
AU  - Bordage, M. -C.
AU  - Breton, V.
AU  - Davídková, Marie
AU  - Delage, E.
AU  - El Bitar, Ziad Ei
AU  - Francis, Ziad
AU  - Guatelli, Susanna
AU  - Ivanchenko, A.
AU  - Ivanchenko, V.
AU  - Karamitros, Mathieu
AU  - Lee, S. B.
AU  - Maigne, Lydia
AU  - Meylan, S.
AU  - Murakami, Kouichi
AU  - Nieminen, P.
AU  - Payno, H.
AU  - Perrot, Yann
AU  - Petrović, Ivan M.
AU  - Pham, Q. T.
AU  - Ristić-Fira, Aleksandra
AU  - Santin, G.
AU  - Sasaki, Takashi
AU  - Seznec, H.
AU  - Shin, J. I.
AU  - Stepan, Vaclav
AU  - Tran, Hoang Ngoc
AU  - Villagrasa, Carmen
AU  - Geant4-DNA Collaboration
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6073
AB  - An accurate modeling of radial energy deposition around ion tracks is a key requirement of radiation transport software used for simulations in radiobiology at the sub-cellular scale. The work presented in this paper is part of the on-going benchmarking of the Geant4-DNA physics processes and models, which are available in the Geant4 Monte Carlo simulation toolkit for the low energy transport of particles in liquid water. We present for the first time radial dose distributions of incident ion tracks simulated with Geant4-DNA. Simulation results are compared to other results available in the literature, obtained from analytical calculations, step-by-step Monte Carlo simulations and measurements. They show a reasonable agreement with reference data. (C) 2014 Elsevier B.V. All rights reserved.
T2  - Nuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
T1  - Simulating radial dose of ion tracks in liquid water simulated with Geant4-DNA: A comparative study
VL  - 333
SP  - 92
EP  - 98
DO  - 10.1016/j.nimb.2014.04.025
ER  - 

@article{
author = "Incerti, Sebastien and Psaltaki, M. and Gillet, P. and Barberet, Ph. and Bardies, M. and Bernal, Mario A. and Bordage, M. -C. and Breton, V. and Davídková, Marie and Delage, E. and El Bitar, Ziad Ei and Francis, Ziad and Guatelli, Susanna and Ivanchenko, A. and Ivanchenko, V. and Karamitros, Mathieu and Lee, S. B. and Maigne, Lydia and Meylan, S. and Murakami, Kouichi and Nieminen, P. and Payno, H. and Perrot, Yann and Petrović, Ivan M. and Pham, Q. T. and Ristić-Fira, Aleksandra and Santin, G. and Sasaki, Takashi and Seznec, H. and Shin, J. I. and Stepan, Vaclav and Tran, Hoang Ngoc and Villagrasa, Carmen and Geant4-DNA Collaboration",
year = "2014",
abstract = "An accurate modeling of radial energy deposition around ion tracks is a key requirement of radiation transport software used for simulations in radiobiology at the sub-cellular scale. The work presented in this paper is part of the on-going benchmarking of the Geant4-DNA physics processes and models, which are available in the Geant4 Monte Carlo simulation toolkit for the low energy transport of particles in liquid water. We present for the first time radial dose distributions of incident ion tracks simulated with Geant4-DNA. Simulation results are compared to other results available in the literature, obtained from analytical calculations, step-by-step Monte Carlo simulations and measurements. They show a reasonable agreement with reference data. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Nuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms",
title = "Simulating radial dose of ion tracks in liquid water simulated with Geant4-DNA: A comparative study",
volume = "333",
pages = "92-98",
doi = "10.1016/j.nimb.2014.04.025"
}

Incerti, S., Psaltaki, M., Gillet, P., Barberet, Ph., Bardies, M., Bernal, M. A., Bordage, M. -C., Breton, V., Davídková, M., Delage, E., El Bitar, Z. E., Francis, Z., Guatelli, S., Ivanchenko, A., Ivanchenko, V., Karamitros, M., Lee, S. B., Maigne, L., Meylan, S., Murakami, K., Nieminen, P., Payno, H., Perrot, Y., Petrović, I. M., Pham, Q. T., Ristić-Fira, A., Santin, G., Sasaki, T., Seznec, H., Shin, J. I., Stepan, V., Tran, H. N., Villagrasa, C.,& Geant4-DNA Collaboration. (2014). Simulating radial dose of ion tracks in liquid water simulated with Geant4-DNA: A comparative study. in Nuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms, 333, 92-98.
https://doi.org/10.1016/j.nimb.2014.04.025

Incerti S, Psaltaki M, Gillet P, Barberet P, Bardies M, Bernal MA, Bordage M-, Breton V, Davídková M, Delage E, El Bitar ZE, Francis Z, Guatelli S, Ivanchenko A, Ivanchenko V, Karamitros M, Lee SB, Maigne L, Meylan S, Murakami K, Nieminen P, Payno H, Perrot Y, Petrović IM, Pham QT, Ristić-Fira A, Santin G, Sasaki T, Seznec H, Shin JI, Stepan V, Tran HN, Villagrasa C, Geant4-DNA Collaboration. Simulating radial dose of ion tracks in liquid water simulated with Geant4-DNA: A comparative study. in Nuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms. 2014;333:92-98.
doi:10.1016/j.nimb.2014.04.025 .

Incerti, Sebastien, Psaltaki, M., Gillet, P., Barberet, Ph., Bardies, M., Bernal, Mario A., Bordage, M. -C., Breton, V., Davídková, Marie, Delage, E., El Bitar, Ziad Ei, Francis, Ziad, Guatelli, Susanna, Ivanchenko, A., Ivanchenko, V., Karamitros, Mathieu, Lee, S. B., Maigne, Lydia, Meylan, S., Murakami, Kouichi, Nieminen, P., Payno, H., Perrot, Yann, Petrović, Ivan M., Pham, Q. T., Ristić-Fira, Aleksandra, Santin, G., Sasaki, Takashi, Seznec, H., Shin, J. I., Stepan, Vaclav, Tran, Hoang Ngoc, Villagrasa, Carmen, Geant4-DNA Collaboration, "Simulating radial dose of ion tracks in liquid water simulated with Geant4-DNA: A comparative study" in Nuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms, 333 (2014):92-98,
https://doi.org/10.1016/j.nimb.2014.04.025 . .

TY  - JOUR
AU  - Romano, Francesco
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Di Rosa, F.
AU  - Mazzaglia, S. E.
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Varisano, A.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6037
AB  - Fluence, depth absorbed dose and linear energy transfer (LET) distributions of proton and carbon ion beams have been investigated using the Monte Carlo code Geant4 (GEometry ANd Tracking). An open source application was developed with the aim to simulate two typical transport beam lines, one used for ocular therapy and cell irradiations with protons and the other for cell irradiations with carbon ions. This tool allows evaluation of the primary and total dose averaged LET and predict their spatial distribution in voxelized or sliced geometries. In order to reproduce the LET distributions in a realistic way, and also the secondary particles contributions due to nuclear interactions were considered in the computations. Pristine and spread-out Bragg peaks were taken into account both for proton and carbon ion beams, with the maximum energy of 62 MeV/n. Depth dose distributions were compared with experimental data, showing good agreement. Primary and total LET distributions were analysed in order to study the influence of contributions of secondary particles in regions at different depths. A non-negligible influence of high-LET components was found in the entrance channel for proton beams, determining the total dose averaged LET by the factor 3 higher than the primary one. A completely different situation was obtained for carbon ions. In this case, secondary particles mainly contributed in the tail that is after the peak. The results showed how the weight of light and heavy secondary ions can considerably influence the computation of LET depth distributions. This has an important role in the interpretation of results coming from radiobiological experiments and, therefore, in hadron treatment planning procedures.
T2  - Physics in Medicine and Biology
T1  - A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line
VL  - 59
IS  - 12
SP  - 2863
EP  - 2882
DO  - 10.1088/0031-9155/59/12/2863
ER  - 

@article{
author = "Romano, Francesco and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Di Rosa, F. and Mazzaglia, S. E. and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Varisano, A.",
year = "2014",
abstract = "Fluence, depth absorbed dose and linear energy transfer (LET) distributions of proton and carbon ion beams have been investigated using the Monte Carlo code Geant4 (GEometry ANd Tracking). An open source application was developed with the aim to simulate two typical transport beam lines, one used for ocular therapy and cell irradiations with protons and the other for cell irradiations with carbon ions. This tool allows evaluation of the primary and total dose averaged LET and predict their spatial distribution in voxelized or sliced geometries. In order to reproduce the LET distributions in a realistic way, and also the secondary particles contributions due to nuclear interactions were considered in the computations. Pristine and spread-out Bragg peaks were taken into account both for proton and carbon ion beams, with the maximum energy of 62 MeV/n. Depth dose distributions were compared with experimental data, showing good agreement. Primary and total LET distributions were analysed in order to study the influence of contributions of secondary particles in regions at different depths. A non-negligible influence of high-LET components was found in the entrance channel for proton beams, determining the total dose averaged LET by the factor 3 higher than the primary one. A completely different situation was obtained for carbon ions. In this case, secondary particles mainly contributed in the tail that is after the peak. The results showed how the weight of light and heavy secondary ions can considerably influence the computation of LET depth distributions. This has an important role in the interpretation of results coming from radiobiological experiments and, therefore, in hadron treatment planning procedures.",
journal = "Physics in Medicine and Biology",
title = "A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line",
volume = "59",
number = "12",
pages = "2863-2882",
doi = "10.1088/0031-9155/59/12/2863"
}

Romano, F., Cirrone, G. A. P., Cuttone, G., Di Rosa, F., Mazzaglia, S. E., Petrović, I. M., Ristić-Fira, A.,& Varisano, A.. (2014). A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line. in Physics in Medicine and Biology, 59(12), 2863-2882.
https://doi.org/10.1088/0031-9155/59/12/2863

Romano F, Cirrone GAP, Cuttone G, Di Rosa F, Mazzaglia SE, Petrović IM, Ristić-Fira A, Varisano A. A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line. in Physics in Medicine and Biology. 2014;59(12):2863-2882.
doi:10.1088/0031-9155/59/12/2863 .

Romano, Francesco, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Di Rosa, F., Mazzaglia, S. E., Petrović, Ivan M., Ristić-Fira, Aleksandra, Varisano, A., "A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line" in Physics in Medicine and Biology, 59, no. 12 (2014):2863-2882,
https://doi.org/10.1088/0031-9155/59/12/2863 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cuttone, Giacomo
AU  - Privitera, Giuseppe
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/167
AB  - Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of the epidermal growth factor receptor in cell proliferation and survival, there are indications that targeted agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance the antitumor treatment by radiation. The aim of this study is to analyze the inactivation effects of gamma-rays and to test the radiosensitizing potential of erlotinib on human lung adenocarcinoma cells in vitro. Irradiations were performed with doses ranging from 1 Gy to 8 Gy. In order to increase the radiosensitivity of CRL-5876 lung adenocarcinoma cells, the cells were treated with a clinically relevant concentration of 2 mu M erlotinib. The effects of single and combined treatments were monitored using clonogenic survival, cell viability and proliferation assays at different time points. For the detection and visualization of the phosphorylated histone H2AX (gamma-H2AX), an important biological marker of DNA double-strand break formation, fluorescence inununocytochemistry, was performed. The response to the treatment was monitored at four time points: 30 min, 2, 6, and 24 h. Irradiations with gamma-rays resulted in significant cell inactivation regarding all analyzed biological endpoints. Combined treatments revealed consistent cell inactivation. Moreover, compared to gamma-rays alone, elevated levels of gamma-H2AX foci were observed after pretreatment with erlotinib, indicating radiosensitization through impaired DNA repair.
T2  - Nuclear technology and radiation protection
T1  - Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition
VL  - 29
IS  - 3
SP  - 233
EP  - 241
DO  - 10.2298/NTRP1403233K
ER  - 

@article{
author = "Keta, Otilija D. and Bulat, Tanja M. and Korićanac, Lela and Žakula, Jelena and Cuttone, Giacomo and Privitera, Giuseppe and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2014",
abstract = "Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of the epidermal growth factor receptor in cell proliferation and survival, there are indications that targeted agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance the antitumor treatment by radiation. The aim of this study is to analyze the inactivation effects of gamma-rays and to test the radiosensitizing potential of erlotinib on human lung adenocarcinoma cells in vitro. Irradiations were performed with doses ranging from 1 Gy to 8 Gy. In order to increase the radiosensitivity of CRL-5876 lung adenocarcinoma cells, the cells were treated with a clinically relevant concentration of 2 mu M erlotinib. The effects of single and combined treatments were monitored using clonogenic survival, cell viability and proliferation assays at different time points. For the detection and visualization of the phosphorylated histone H2AX (gamma-H2AX), an important biological marker of DNA double-strand break formation, fluorescence inununocytochemistry, was performed. The response to the treatment was monitored at four time points: 30 min, 2, 6, and 24 h. Irradiations with gamma-rays resulted in significant cell inactivation regarding all analyzed biological endpoints. Combined treatments revealed consistent cell inactivation. Moreover, compared to gamma-rays alone, elevated levels of gamma-H2AX foci were observed after pretreatment with erlotinib, indicating radiosensitization through impaired DNA repair.",
journal = "Nuclear technology and radiation protection",
title = "Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition",
volume = "29",
number = "3",
pages = "233-241",
doi = "10.2298/NTRP1403233K"
}

Keta, O. D., Bulat, T. M., Korićanac, L., Žakula, J., Cuttone, G., Privitera, G., Petrović, I. M.,& Ristić-Fira, A.. (2014). Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition. in Nuclear technology and radiation protection, 29(3), 233-241.
https://doi.org/10.2298/NTRP1403233K

Keta OD, Bulat TM, Korićanac L, Žakula J, Cuttone G, Privitera G, Petrović IM, Ristić-Fira A. Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition. in Nuclear technology and radiation protection. 2014;29(3):233-241.
doi:10.2298/NTRP1403233K .

Keta, Otilija D., Bulat, Tanja M., Korićanac, Lela, Žakula, Jelena, Cuttone, Giacomo, Privitera, Giuseppe, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition" in Nuclear technology and radiation protection, 29, no. 3 (2014):233-241,
https://doi.org/10.2298/NTRP1403233K . .

TY  - CONF
AU  - Ristić-Fira, Aleksandra
AU  - Bulat, Tanja M.
AU  - Keta, Otilija D.
AU  - Romano, Francesco
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7005
AB  - The aim of this study is to investigate the behavior of radio-resistant human malignant cells, thus enabling better understanding of radiobiological effects of ions in such a case. Radiation sources such as accelerated continuous ion beams and laser technology-based ultra short radiation sources with energy of around 10 MeV will be used. The HTB140 melanoma cells are chosen since it has been shown that they represent the limit case of cellular radio-resistance among the studied tumour cell lines. These cells are particularly interesting as they provide data on the very edge of inactivation capacity of each beam line that is tested. After exposing the cell monolayers to continuous radiations of low (gamma-rays) and high (protons) linear energy transfer, the kinetics of disappearance of the phosphorylated histone H2AX (gamma-H2AX) foci per cell will be determined. The same procedure will be performed with the pulsed high dose rate protons. Detection and quantification of gamma-H2AX foci will be performed by immunohistochemical 3D time-dependent imaging analyses using laser scanning confocal microscopy. Immunoblotting will enable the follow-up of the relation between gamma-H2AX and cell cycle arrest via the p53/p21 pathway. In such a way the spatio-temporal changes on sub-cellular level will be visualized, quantified and compared. These results will show whether there is a difference in the effects on cells between continuous and pulsed irradiation mode. Therefore, they will contribute to the database that might promote pulsed sources for medical treatments of malignant growths.
C3  - AIP Conference Proceedings
T1  - Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED
VL  - 1546
SP  - 101
EP  - 104
DO  - 10.1063/1.4816616
ER  - 

@conference{
author = "Ristić-Fira, Aleksandra and Bulat, Tanja M. and Keta, Otilija D. and Romano, Francesco and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2013",
abstract = "The aim of this study is to investigate the behavior of radio-resistant human malignant cells, thus enabling better understanding of radiobiological effects of ions in such a case. Radiation sources such as accelerated continuous ion beams and laser technology-based ultra short radiation sources with energy of around 10 MeV will be used. The HTB140 melanoma cells are chosen since it has been shown that they represent the limit case of cellular radio-resistance among the studied tumour cell lines. These cells are particularly interesting as they provide data on the very edge of inactivation capacity of each beam line that is tested. After exposing the cell monolayers to continuous radiations of low (gamma-rays) and high (protons) linear energy transfer, the kinetics of disappearance of the phosphorylated histone H2AX (gamma-H2AX) foci per cell will be determined. The same procedure will be performed with the pulsed high dose rate protons. Detection and quantification of gamma-H2AX foci will be performed by immunohistochemical 3D time-dependent imaging analyses using laser scanning confocal microscopy. Immunoblotting will enable the follow-up of the relation between gamma-H2AX and cell cycle arrest via the p53/p21 pathway. In such a way the spatio-temporal changes on sub-cellular level will be visualized, quantified and compared. These results will show whether there is a difference in the effects on cells between continuous and pulsed irradiation mode. Therefore, they will contribute to the database that might promote pulsed sources for medical treatments of malignant growths.",
journal = "AIP Conference Proceedings",
title = "Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED",
volume = "1546",
pages = "101-104",
doi = "10.1063/1.4816616"
}

Ristić-Fira, A., Bulat, T. M., Keta, O. D., Romano, F., Cirrone, G. A. P., Cuttone, G.,& Petrović, I. M.. (2013). Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED. in AIP Conference Proceedings, 1546, 101-104.
https://doi.org/10.1063/1.4816616

Ristić-Fira A, Bulat TM, Keta OD, Romano F, Cirrone GAP, Cuttone G, Petrović IM. Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED. in AIP Conference Proceedings. 2013;1546:101-104.
doi:10.1063/1.4816616 .

Ristić-Fira, Aleksandra, Bulat, Tanja M., Keta, Otilija D., Romano, Francesco, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Petrović, Ivan M., "Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED" in AIP Conference Proceedings, 1546 (2013):101-104,
https://doi.org/10.1063/1.4816616 . .

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5593
AB  - This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation.
T2  - Nuclear technology and radiation protection
T1  - Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells
VL  - 28
IS  - 2
SP  - 195
EP  - 203
DO  - 10.2298/NTRP1302195K
ER  - 

@article{
author = "Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2013",
abstract = "This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation.",
journal = "Nuclear technology and radiation protection",
title = "Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells",
volume = "28",
number = "2",
pages = "195-203",
doi = "10.2298/NTRP1302195K"
}

Korićanac, L., Žakula, J., Keta, O. D., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2013). Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells. in Nuclear technology and radiation protection, 28(2), 195-203.
https://doi.org/10.2298/NTRP1302195K

Korićanac L, Žakula J, Keta OD, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells. in Nuclear technology and radiation protection. 2013;28(2):195-203.
doi:10.2298/NTRP1302195K .

Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells" in Nuclear technology and radiation protection, 28, no. 2 (2013):195-203,
https://doi.org/10.2298/NTRP1302195K . .

TY  - CONF
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Todorović, Dragana
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8642
C3  - Radiotherapy and Oncology
T1  - Response of human lung adenocarcinoma cells to proton radiation and erlotinib
VL  - 102
SP  - S106
EP  - S107
DO  - 10.1016/S0167-8140(12)70182-2
ER  - 

@conference{
author = "Ristić-Fira, Aleksandra and Petrović, Ivan M. and Todorović, Dragana and Korićanac, Lela and Keta, Otilija D. and Bulat, Tanja M. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo",
year = "2012",
journal = "Radiotherapy and Oncology",
title = "Response of human lung adenocarcinoma cells to proton radiation and erlotinib",
volume = "102",
pages = "S106-S107",
doi = "10.1016/S0167-8140(12)70182-2"
}

Ristić-Fira, A., Petrović, I. M., Todorović, D., Korićanac, L., Keta, O. D., Bulat, T. M., Cirrone, G. A. P., Romano, F.,& Cuttone, G.. (2012). Response of human lung adenocarcinoma cells to proton radiation and erlotinib. in Radiotherapy and Oncology, 102, S106-S107.
https://doi.org/10.1016/S0167-8140(12)70182-2

Ristić-Fira A, Petrović IM, Todorović D, Korićanac L, Keta OD, Bulat TM, Cirrone GAP, Romano F, Cuttone G. Response of human lung adenocarcinoma cells to proton radiation and erlotinib. in Radiotherapy and Oncology. 2012;102:S106-S107.
doi:10.1016/S0167-8140(12)70182-2 .

Ristić-Fira, Aleksandra, Petrović, Ivan M., Todorović, Dragana, Korićanac, Lela, Keta, Otilija D., Bulat, Tanja M., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, "Response of human lung adenocarcinoma cells to proton radiation and erlotinib" in Radiotherapy and Oncology, 102 (2012):S106-S107,
https://doi.org/10.1016/S0167-8140(12)70182-2 . .

TY  - CONF
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Dragana
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8643
C3  - Radiotherapy and Oncology
T1  - Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET
VL  - 102
SP  - S108
EP  - S109
DO  - 10.1016/S0167-8140(12)70185-8
ER  - 

@conference{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Dragana and Korićanac, Lela and Žakula, Jelena and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo",
year = "2012",
journal = "Radiotherapy and Oncology",
title = "Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET",
volume = "102",
pages = "S108-S109",
doi = "10.1016/S0167-8140(12)70185-8"
}

Petrović, I. M., Ristić-Fira, A., Todorović, D., Korićanac, L., Žakula, J., Cirrone, G. A. P., Romano, F.,& Cuttone, G.. (2012). Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET. in Radiotherapy and Oncology, 102, S108-S109.
https://doi.org/10.1016/S0167-8140(12)70185-8

Petrović IM, Ristić-Fira A, Todorović D, Korićanac L, Žakula J, Cirrone GAP, Romano F, Cuttone G. Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET. in Radiotherapy and Oncology. 2012;102:S108-S109.
doi:10.1016/S0167-8140(12)70185-8 .

Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Dragana, Korićanac, Lela, Žakula, Jelena, Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, "Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET" in Radiotherapy and Oncology, 102 (2012):S108-S109,
https://doi.org/10.1016/S0167-8140(12)70185-8 . .

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8644
AB  - The effects of combined treatments with fotemustine and proton radiation on cell proliferation and induction of apoptosis have been analyzed in this study. HTB140 human melanoma cells were treated with fotemustine (100, 250 M) 24 h prior to irradiation (12, 16 Gy). The cells were irradiated in the middle of a therapeutic 62 MeV proton spread-out Bragg peak. An efficiency of applied treatments was observed throughout the evaluation of the cell proliferation 7 days after proton irradiation. The combined treatments with fotemustine and protons resulted in a greater antiproliferative response than each treatment alone. The number of apoptotic cells was estimated after 6 or 48 h using flow cytometry. The highest percentage of apoptotic cells was obtained 48 h after treatment with 250 M fotemustine and protons. Western blot analysis showed that induction of apoptosis was associated with p53 and Bax up regulation, and Bcl-2 down regulation. The induction of a caspase-3 activity and cleavage of PARP were clearly observed. These data indicate that a combined application of FM and proton irradiation is more effective in reducing melanoma cell proliferation and the induction of apoptosis, suggesting that FM can increase the radio-sensitivity of HTB140 melanoma cells.
T2  - Advanced Science Letters
T1  - Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line
VL  - 5
IS  - 2
SP  - 552
EP  - 559
DO  - 10.1166/asl.2012.2150
ER  - 

@article{
author = "Korićanac, Lela and Žakula, Jelena and Cirrone, Giuseppe Antonio Pablo and Privitera, Giuseppe and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2012",
abstract = "The effects of combined treatments with fotemustine and proton radiation on cell proliferation and induction of apoptosis have been analyzed in this study. HTB140 human melanoma cells were treated with fotemustine (100, 250 M) 24 h prior to irradiation (12, 16 Gy). The cells were irradiated in the middle of a therapeutic 62 MeV proton spread-out Bragg peak. An efficiency of applied treatments was observed throughout the evaluation of the cell proliferation 7 days after proton irradiation. The combined treatments with fotemustine and protons resulted in a greater antiproliferative response than each treatment alone. The number of apoptotic cells was estimated after 6 or 48 h using flow cytometry. The highest percentage of apoptotic cells was obtained 48 h after treatment with 250 M fotemustine and protons. Western blot analysis showed that induction of apoptosis was associated with p53 and Bax up regulation, and Bcl-2 down regulation. The induction of a caspase-3 activity and cleavage of PARP were clearly observed. These data indicate that a combined application of FM and proton irradiation is more effective in reducing melanoma cell proliferation and the induction of apoptosis, suggesting that FM can increase the radio-sensitivity of HTB140 melanoma cells.",
journal = "Advanced Science Letters",
title = "Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line",
volume = "5",
number = "2",
pages = "552-559",
doi = "10.1166/asl.2012.2150"
}

Korićanac, L., Žakula, J., Cirrone, G. A. P., Privitera, G., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2012). Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line. in Advanced Science Letters, 5(2), 552-559.
https://doi.org/10.1166/asl.2012.2150

Korićanac L, Žakula J, Cirrone GAP, Privitera G, Cuttone G, Petrović IM, Ristić-Fira A. Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line. in Advanced Science Letters. 2012;5(2):552-559.
doi:10.1166/asl.2012.2150 .

Korićanac, Lela, Žakula, Jelena, Cirrone, Giuseppe Antonio Pablo, Privitera, Giuseppe, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line" in Advanced Science Letters, 5, no. 2 (2012):552-559,
https://doi.org/10.1166/asl.2012.2150 . .