TY  - JOUR
AU  - Vujić, Dragana
AU  - Petrović, Sandra
AU  - Lazić, Emilija
AU  - Kuzmanović, Miloš
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Mićić, Dragan
AU  - Jovanović, Ankica
AU  - Zečević, Željko
AU  - Guć-Šćekić, Marija
AU  - Ćirković, Sanja
AU  - Joksić, Gordana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5928
AB  - To investigate genetic subtypes of inherited bone marrow failure syndrome Fanconi anemia (FA) in Sebia. FA-D2 subtype was found to be the most frequent genetic subtype among investigated FA patients; specific observations of FA-D2 phenotype are pointed out. Several biological endpoints of FA cells in vitro such as radiation-induced level of lymphocyte micronuclei (radiosensitivity), base line and radiation induced level of the DNA double strand breaks (DSBs), leukocyte apoptosis, and telomere capping function were assessed. The results indicate that all FA-D2 patients display radioresistant in vitro response, which is seen as significantly reduced yield of radiation-induced micronuclei. On the contrary, FA-A patients display radiosensitive in vitro response seen as increased number of radiation-induced micronuclei (MN). A massive elimination of irradiated cells via apoptosis is found in both FA-A and FA-D2 subtypes. In FA-A subtype apoptosis positively relates with the yield of radiation-induced MN, whereas in FA-D2 subtype apoptosis relates with a high percentage of cells carrying dysfunctional telomeres. The present results unequivocally demonstrate that cytokinesis-block micronucleus (CBMN) assay and analyses of telomere capping function can be used to distinguish FA-D2 and FA-A complementation groups. Considering all biological endpoints were analyzed, it can be concluded that all FA patients are radiosensitive, regardless of their complementation group. Thus, using CBMN test and telomere capping function analysis can discriminate FA-A from FA-D2 complementation groups, which could be important for assessment the conditioning regimens prior to bone marrow transplantation.
T2  - Indian Journal of Pediatrics
T1  - Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia
VL  - 81
IS  - 3
SP  - 260
EP  - 265
DO  - 10.1007/s12098-013-1284-4
ER  - 

@article{
author = "Vujić, Dragana and Petrović, Sandra and Lazić, Emilija and Kuzmanović, Miloš and Leskovac, Andreja and Joksić, Ivana and Mićić, Dragan and Jovanović, Ankica and Zečević, Željko and Guć-Šćekić, Marija and Ćirković, Sanja and Joksić, Gordana",
year = "2014",
abstract = "To investigate genetic subtypes of inherited bone marrow failure syndrome Fanconi anemia (FA) in Sebia. FA-D2 subtype was found to be the most frequent genetic subtype among investigated FA patients; specific observations of FA-D2 phenotype are pointed out. Several biological endpoints of FA cells in vitro such as radiation-induced level of lymphocyte micronuclei (radiosensitivity), base line and radiation induced level of the DNA double strand breaks (DSBs), leukocyte apoptosis, and telomere capping function were assessed. The results indicate that all FA-D2 patients display radioresistant in vitro response, which is seen as significantly reduced yield of radiation-induced micronuclei. On the contrary, FA-A patients display radiosensitive in vitro response seen as increased number of radiation-induced micronuclei (MN). A massive elimination of irradiated cells via apoptosis is found in both FA-A and FA-D2 subtypes. In FA-A subtype apoptosis positively relates with the yield of radiation-induced MN, whereas in FA-D2 subtype apoptosis relates with a high percentage of cells carrying dysfunctional telomeres. The present results unequivocally demonstrate that cytokinesis-block micronucleus (CBMN) assay and analyses of telomere capping function can be used to distinguish FA-D2 and FA-A complementation groups. Considering all biological endpoints were analyzed, it can be concluded that all FA patients are radiosensitive, regardless of their complementation group. Thus, using CBMN test and telomere capping function analysis can discriminate FA-A from FA-D2 complementation groups, which could be important for assessment the conditioning regimens prior to bone marrow transplantation.",
journal = "Indian Journal of Pediatrics",
title = "Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia",
volume = "81",
number = "3",
pages = "260-265",
doi = "10.1007/s12098-013-1284-4"
}

Vujić, D., Petrović, S., Lazić, E., Kuzmanović, M., Leskovac, A., Joksić, I., Mićić, D., Jovanović, A., Zečević, Ž., Guć-Šćekić, M., Ćirković, S.,& Joksić, G.. (2014). Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia. in Indian Journal of Pediatrics, 81(3), 260-265.
https://doi.org/10.1007/s12098-013-1284-4

Vujić D, Petrović S, Lazić E, Kuzmanović M, Leskovac A, Joksić I, Mićić D, Jovanović A, Zečević Ž, Guć-Šćekić M, Ćirković S, Joksić G. Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia. in Indian Journal of Pediatrics. 2014;81(3):260-265.
doi:10.1007/s12098-013-1284-4 .

Vujić, Dragana, Petrović, Sandra, Lazić, Emilija, Kuzmanović, Miloš, Leskovac, Andreja, Joksić, Ivana, Mićić, Dragan, Jovanović, Ankica, Zečević, Željko, Guć-Šćekić, Marija, Ćirković, Sanja, Joksić, Gordana, "Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia" in Indian Journal of Pediatrics, 81, no. 3 (2014):260-265,
https://doi.org/10.1007/s12098-013-1284-4 . .