Vasić, Vesna M.

Link to this page

Authority KeyName Variants
orcid::0000-0003-1268-2363
  • Vasić, Vesna M. (126)
Projects
Studies of enzyme interactions with toxic and pharmacologically active molecules Istraživanje mehanizma interakcija biološki aktivnih jedinjenja sa biomolekulima
COST Action [MP1302] Electroconducting and redox-active polymers and oligomers: synthesis, structure, properties and applications
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) [1991]
[2700PL309/2003] AIRC [IG-12085], Beneficentia Stiftung Vaduz
Beneficentia Stiftung (Vaduz), ITT (Istituto Toscano Tumori), Fondazione Cassa Risparmio Firenze (CRF), AIRC [IG-16049], AIRC-FIRC (Fondazione Italiana per la Ricerca sul Cancro) [18044] Beneficentia Stiftung (Vaduz, Liechtenstein), AIRC [IG-16049], ITT, Fondazione CRF, CIRCMSB
Carl Tryggers Foundation for Scientific Research CNCSIS [69/2006]
CNCSIS [ET 3264/2005], Ministry of Science and Environmental Protection of Serbia [142051b] COST Action [MP1302 - Nanospectroscopy]
CSC, Wuhan Applied Basic Research Program [2014010101010020] European Cooperation in Science and Technology through COST Action [MP1302]
European Union (EU) [POPW.01.03.00-20-034/09-00] Impact of agents with potential use in functional foods on biomarkers for induction of age related diseases
Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials Structure-properties relationships of natural and synthetic molecules and their metal complexes
Effects of laser radiation and plasma on novel materials in their synthesis, modification, and analysis Hydrogen Energy - Research and Development of New Materials: Electrolytic Hydrogen Production, Hydrogen Fuel Cells, Isotope Effects
Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites Hormonal regulation of expression and activity of the nitric oxide synthase and sodium-potassium pump in experimental models of insulin resistance, diabetes and cardiovascular disorders
Microbial diversity study and characterization of beneficial environmental microorganisms Application of low temperature plasmas in biomedicine, environmental protection and nanotechnologies
Cellular and molecular basis of neuroinflamation: potential targets for translational medicine and therapy Studying climate change and its influence on environment: impacts, adaptation and mitigation
Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety Status, trends and possibilities to increase the fertility of agricultural land in the Vojvodina Province

Author's Bibliography

An insight into the efficient dimethoate adsorption on graphene-based materials—a combined experimental and dft study

Anićijević, Vladan J.; Lazarević-Pašti, Tamara; Vasić, Vesna M.; Vasić-Anićijević, Dragana D.

(2021)

TY  - JOUR
AU  - Anićijević, Vladan J.
AU  - Lazarević-Pašti, Tamara
AU  - Vasić, Vesna M.
AU  - Vasić-Anićijević, Dragana D.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9811
AB  - The development of highly efficient methods for removing hazardous substances from the environment attracts increasing attention. Understanding the basic principles of the removal processes using graphene materials is equally essential to confirm their application efficiency and safety. (2) Methods: In this contribution, adsorption of pesticide dimethoate (DMT) on graphene-based materials has been investigated on the molecular level. (3) Results: The experimental results’ analysis revealed a cooperative binding mechanism of the DMT on the adsorption sites of investigated materials—graphene oxide (GO) and industrial graphene (IG). The adsorption data were analyzed using various adsorption isotherms to determine the thermodynamics of the adsorption process. The experimental results were correlated with Density Functional Theory (DFT) calculations of DMT adsorption on the model surfaces that appropriately describe the graphene materials’ reactive features. (4) Conclusions: Considering experimental results, calculated adsorption energies, optimized adsorption geometries, and electronic structure, it was proposed that the dispersive interactions determine the adsorption properties of DMT on plain graphene sites (physisorption). Additionally, it was shown that the existence of vacancy-type defect sites on the surfaces could induce strong and dissociative adsorption (chemisorption) of DMT. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
T2  - Applied Sciences (Switzerland)
T1  - An insight into the efficient dimethoate adsorption on graphene-based materials—a combined experimental and dft study
VL  - 11
IS  - 9
DO  - 10.3390/app11094014
ER  - 
@article{
author = "Anićijević, Vladan J. and Lazarević-Pašti, Tamara and Vasić, Vesna M. and Vasić-Anićijević, Dragana D.",
year = "2021",
abstract = "The development of highly efficient methods for removing hazardous substances from the environment attracts increasing attention. Understanding the basic principles of the removal processes using graphene materials is equally essential to confirm their application efficiency and safety. (2) Methods: In this contribution, adsorption of pesticide dimethoate (DMT) on graphene-based materials has been investigated on the molecular level. (3) Results: The experimental results’ analysis revealed a cooperative binding mechanism of the DMT on the adsorption sites of investigated materials—graphene oxide (GO) and industrial graphene (IG). The adsorption data were analyzed using various adsorption isotherms to determine the thermodynamics of the adsorption process. The experimental results were correlated with Density Functional Theory (DFT) calculations of DMT adsorption on the model surfaces that appropriately describe the graphene materials’ reactive features. (4) Conclusions: Considering experimental results, calculated adsorption energies, optimized adsorption geometries, and electronic structure, it was proposed that the dispersive interactions determine the adsorption properties of DMT on plain graphene sites (physisorption). Additionally, it was shown that the existence of vacancy-type defect sites on the surfaces could induce strong and dissociative adsorption (chemisorption) of DMT. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
journal = "Applied Sciences (Switzerland)",
title = "An insight into the efficient dimethoate adsorption on graphene-based materials—a combined experimental and dft study",
volume = "11",
number = "9",
doi = "10.3390/app11094014"
}
Anićijević, V. J., Lazarević-Pašti, T., Vasić, V. M.,& Vasić-Anićijević, D. D.. (2021). An insight into the efficient dimethoate adsorption on graphene-based materials—a combined experimental and dft study. in Applied Sciences (Switzerland), 11(9).
https://doi.org/10.3390/app11094014
Anićijević VJ, Lazarević-Pašti T, Vasić VM, Vasić-Anićijević DD. An insight into the efficient dimethoate adsorption on graphene-based materials—a combined experimental and dft study. in Applied Sciences (Switzerland). 2021;11(9).
doi:10.3390/app11094014 .
Anićijević, Vladan J., Lazarević-Pašti, Tamara, Vasić, Vesna M., Vasić-Anićijević, Dragana D., "An insight into the efficient dimethoate adsorption on graphene-based materials—a combined experimental and dft study" in Applied Sciences (Switzerland), 11, no. 9 (2021),
https://doi.org/10.3390/app11094014 . .

Drug delivery systems based on nanoparticles and related nanostructures

Vujačić Nikezić, Ana V.; Bondžić, Aleksandra M.; Vasić, Vesna M.

(2020)

TY  - JOUR
AU  - Vujačić Nikezić, Ana V.
AU  - Bondžić, Aleksandra M.
AU  - Vasić, Vesna M.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9049
AB  - A new approach to drug design based on nanoparticles and related nanostructures for effective drug delivery, is of great importance in future medical treatment, especially for cancer therapy. Nanomaterials hold tremendous potential for increasing the efficiency of drug delivery, with a high degree of biocompatibility. Additionally, for biomedical applications, they must be biodegradable, have prolonged circulation half-life, not tend to aggregate or cause an inflammatory response in the body and to be cost-effective. The efficacy of such structures is highly dependent on their chemical properties as well as on shape, charge, size, surface modifications and loading method. Here we focused on the potential of using different kinds of nanoparticles and similar nanostructures loaded with various drugs in order to achieve specific targeting and controlled drug release. Thereby, computational modeling on NPs-based drug delivery could help in providing a better understanding of all parts of the delivery system. This review emphasizes recent advances in the usage of various types of nanoparticles and similar nanostructures for drug delivery, aiming to provide a critical review of less toxic and more effective treatment.
T2  - European Journal of Pharmaceutical Sciences
T1  - Drug delivery systems based on nanoparticles and related nanostructures
VL  - 151
SP  - 105412
DO  - 10.1016/j.ejps.2020.105412
ER  - 
@article{
author = "Vujačić Nikezić, Ana V. and Bondžić, Aleksandra M. and Vasić, Vesna M.",
year = "2020",
abstract = "A new approach to drug design based on nanoparticles and related nanostructures for effective drug delivery, is of great importance in future medical treatment, especially for cancer therapy. Nanomaterials hold tremendous potential for increasing the efficiency of drug delivery, with a high degree of biocompatibility. Additionally, for biomedical applications, they must be biodegradable, have prolonged circulation half-life, not tend to aggregate or cause an inflammatory response in the body and to be cost-effective. The efficacy of such structures is highly dependent on their chemical properties as well as on shape, charge, size, surface modifications and loading method. Here we focused on the potential of using different kinds of nanoparticles and similar nanostructures loaded with various drugs in order to achieve specific targeting and controlled drug release. Thereby, computational modeling on NPs-based drug delivery could help in providing a better understanding of all parts of the delivery system. This review emphasizes recent advances in the usage of various types of nanoparticles and similar nanostructures for drug delivery, aiming to provide a critical review of less toxic and more effective treatment.",
journal = "European Journal of Pharmaceutical Sciences",
title = "Drug delivery systems based on nanoparticles and related nanostructures",
volume = "151",
pages = "105412",
doi = "10.1016/j.ejps.2020.105412"
}
Vujačić Nikezić, A. V., Bondžić, A. M.,& Vasić, V. M.. (2020). Drug delivery systems based on nanoparticles and related nanostructures. in European Journal of Pharmaceutical Sciences, 151, 105412.
https://doi.org/10.1016/j.ejps.2020.105412
Vujačić Nikezić AV, Bondžić AM, Vasić VM. Drug delivery systems based on nanoparticles and related nanostructures. in European Journal of Pharmaceutical Sciences. 2020;151:105412.
doi:10.1016/j.ejps.2020.105412 .
Vujačić Nikezić, Ana V., Bondžić, Aleksandra M., Vasić, Vesna M., "Drug delivery systems based on nanoparticles and related nanostructures" in European Journal of Pharmaceutical Sciences, 151 (2020):105412,
https://doi.org/10.1016/j.ejps.2020.105412 . .
1
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16

Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles

Laban, Bojana B.; Ralević, Uroš; Petrović, Sandra; Leskovac, Andreja; Vasić Anićijević, Dragana D.; Marković, Mirjana; Vasić, Vesna M.

(2020)

TY  - JOUR
AU  - Laban, Bojana B.
AU  - Ralević, Uroš
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Vasić Anićijević, Dragana D.
AU  - Marković, Mirjana
AU  - Vasić, Vesna M.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8673
AB  - The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019
T2  - Journal of Inorganic Biochemistry
T1  - Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles
VL  - 204
SP  - 110958
DO  - 10.1016/j.jinorgbio.2019.110958
ER  - 
@article{
author = "Laban, Bojana B. and Ralević, Uroš and Petrović, Sandra and Leskovac, Andreja and Vasić Anićijević, Dragana D. and Marković, Mirjana and Vasić, Vesna M.",
year = "2020",
abstract = "The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019",
journal = "Journal of Inorganic Biochemistry",
title = "Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles",
volume = "204",
pages = "110958",
doi = "10.1016/j.jinorgbio.2019.110958"
}
Laban, B. B., Ralević, U., Petrović, S., Leskovac, A., Vasić Anićijević, D. D., Marković, M.,& Vasić, V. M.. (2020). Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles. in Journal of Inorganic Biochemistry, 204, 110958.
https://doi.org/10.1016/j.jinorgbio.2019.110958
Laban BB, Ralević U, Petrović S, Leskovac A, Vasić Anićijević DD, Marković M, Vasić VM. Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles. in Journal of Inorganic Biochemistry. 2020;204:110958.
doi:10.1016/j.jinorgbio.2019.110958 .
Laban, Bojana B., Ralević, Uroš, Petrović, Sandra, Leskovac, Andreja, Vasić Anićijević, Dragana D., Marković, Mirjana, Vasić, Vesna M., "Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles" in Journal of Inorganic Biochemistry, 204 (2020):110958,
https://doi.org/10.1016/j.jinorgbio.2019.110958 . .
14
10
12

Non-thermal plasma needle as an effective tool in dimethoate removal from water

Mitrović, Tatjana; Lazović, Saša; Nastasijević, Branislav J.; Pašti, Igor A.; Vasić, Vesna M.; Lazarević-Pašti, Tamara

(2019)

TY  - JOUR
AU  - Mitrović, Tatjana
AU  - Lazović, Saša
AU  - Nastasijević, Branislav J.
AU  - Pašti, Igor A.
AU  - Vasić, Vesna M.
AU  - Lazarević-Pašti, Tamara
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0301479719307716
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8337
AB  - Intensive use of pesticides requires innovative approaches for their removal from the environment. Here we report the method for degradation of dimethoate in water using non-thermal plasma needle and analyze kinetics of dimethoate removal and possible degradation pathways. The effects of dimethoate initial concentration, plasma treatment time, Argon flow rate and the presence of radical promoters on the effectiveness of proposed method are evaluated. With argon flow rate of 0.5 slm (standard litres per minute) 1 × 10−4 M dimethoate can be removed within 30 min of treatment. Using UPLC analysis it was confirmed that one of the decomposition products is dimethoate oxo-analogue omethoate, which is in fact more toxic than dimethoate. However, the overall toxicity of contaminated water was reduced upon the treatment. The addition of H2O2 as a free radical promoter enhances dimethoate removal, while K2S2O8 results with selective conversion to omethoate. Using mass spectrometry in combination with the theoretical calculations, possible degradation pathways were proposed. The feasibility of the proposed method for dimethoate degradation in real water samples is confirmed. The proposed method is demonstrated as a highly effective approach for dimethoate removal without significant accumulation of undesirable toxic products and secondary waste. © 2019 Elsevier Ltd
T2  - Journal of Environmental Management
T1  - Non-thermal plasma needle as an effective tool in dimethoate removal from water
VL  - 246
SP  - 63
EP  - 70
DO  - 10.1016/j.jenvman.2019.05.143
ER  - 
@article{
author = "Mitrović, Tatjana and Lazović, Saša and Nastasijević, Branislav J. and Pašti, Igor A. and Vasić, Vesna M. and Lazarević-Pašti, Tamara",
year = "2019",
abstract = "Intensive use of pesticides requires innovative approaches for their removal from the environment. Here we report the method for degradation of dimethoate in water using non-thermal plasma needle and analyze kinetics of dimethoate removal and possible degradation pathways. The effects of dimethoate initial concentration, plasma treatment time, Argon flow rate and the presence of radical promoters on the effectiveness of proposed method are evaluated. With argon flow rate of 0.5 slm (standard litres per minute) 1 × 10−4 M dimethoate can be removed within 30 min of treatment. Using UPLC analysis it was confirmed that one of the decomposition products is dimethoate oxo-analogue omethoate, which is in fact more toxic than dimethoate. However, the overall toxicity of contaminated water was reduced upon the treatment. The addition of H2O2 as a free radical promoter enhances dimethoate removal, while K2S2O8 results with selective conversion to omethoate. Using mass spectrometry in combination with the theoretical calculations, possible degradation pathways were proposed. The feasibility of the proposed method for dimethoate degradation in real water samples is confirmed. The proposed method is demonstrated as a highly effective approach for dimethoate removal without significant accumulation of undesirable toxic products and secondary waste. © 2019 Elsevier Ltd",
journal = "Journal of Environmental Management",
title = "Non-thermal plasma needle as an effective tool in dimethoate removal from water",
volume = "246",
pages = "63-70",
doi = "10.1016/j.jenvman.2019.05.143"
}
Mitrović, T., Lazović, S., Nastasijević, B. J., Pašti, I. A., Vasić, V. M.,& Lazarević-Pašti, T.. (2019). Non-thermal plasma needle as an effective tool in dimethoate removal from water. in Journal of Environmental Management, 246, 63-70.
https://doi.org/10.1016/j.jenvman.2019.05.143
Mitrović T, Lazović S, Nastasijević BJ, Pašti IA, Vasić VM, Lazarević-Pašti T. Non-thermal plasma needle as an effective tool in dimethoate removal from water. in Journal of Environmental Management. 2019;246:63-70.
doi:10.1016/j.jenvman.2019.05.143 .
Mitrović, Tatjana, Lazović, Saša, Nastasijević, Branislav J., Pašti, Igor A., Vasić, Vesna M., Lazarević-Pašti, Tamara, "Non-thermal plasma needle as an effective tool in dimethoate removal from water" in Journal of Environmental Management, 246 (2019):63-70,
https://doi.org/10.1016/j.jenvman.2019.05.143 . .
16
11
17

Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue

Bondžić, Aleksandra M.; Leskovac, Andreja; Petrović, Sandra; Vasić Anićijević, Dragana D.; Luce, Marco; Massai, Lara; Generosi, Amanda; Paci, Barbara; Cricenti, Antonio; Messori, Luigi; Vasić, Vesna M.

(2019)

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Vasić Anićijević, Dragana D.
AU  - Luce, Marco
AU  - Massai, Lara
AU  - Generosi, Amanda
AU  - Paci, Barbara
AU  - Cricenti, Antonio
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8747
AB  - Citrate-capped gold nanoparticles (AuNPs) were functionalized with three distinct antitumor gold(III) complexes, e.g., [Au(N,N)(OH)2][PF6], where (N,N)=2,2′-bipyridine; [Au(C,N)(AcO)2], where (C,N)=deprotonated 6-(1,1-dimethylbenzyl)-pyridine; [Au(C,N,N)(OH)][PF6], where (C,N,N)=deprotonated 6-(1,1-dimethylbenzyl)-2,2′-bipyridine, to assess the chance of tracking their subcellular distribution by atomic force microscopy (AFM), and surface enhanced Raman spectroscopy (SERS) techniques. An extensive physicochemical characterization of the formed conjugates was, thus, carried out by applying a variety of methods (density functional theory—DFT, UV/Vis spectrophotometry, AFM, Raman spectroscopy, and SERS). The resulting gold(III) complexes/AuNPs conjugates turned out to be pretty stable. Interestingly, they exhibited a dramatically increased resonance intensity in the Raman spectra induced by AuNPs. For testing the use of the functionalized AuNPs for biosensing, their distribution in the nuclear, cytosolic, and membrane cell fractions obtained from human lymphocytes was investigated by AFM and SERS. The conjugates were detected in the membrane and nuclear cell fractions but not in the cytosol. The AFM method confirmed that conjugates induced changes in the morphology and nanostructure of the membrane and nuclear fractions. The obtained results point out that the conjugates formed between AuNPs and gold(III) complexes may be used as a tool for tracking metallodrug distribution in the different cell fractions.
T2  - International Journal of Molecular Sciences
T1  - Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue
VL  - 20
IS  - 24
SP  - 6306
DO  - 10.3390/ijms20246306
ER  - 
@article{
author = "Bondžić, Aleksandra M. and Leskovac, Andreja and Petrović, Sandra and Vasić Anićijević, Dragana D. and Luce, Marco and Massai, Lara and Generosi, Amanda and Paci, Barbara and Cricenti, Antonio and Messori, Luigi and Vasić, Vesna M.",
year = "2019",
abstract = "Citrate-capped gold nanoparticles (AuNPs) were functionalized with three distinct antitumor gold(III) complexes, e.g., [Au(N,N)(OH)2][PF6], where (N,N)=2,2′-bipyridine; [Au(C,N)(AcO)2], where (C,N)=deprotonated 6-(1,1-dimethylbenzyl)-pyridine; [Au(C,N,N)(OH)][PF6], where (C,N,N)=deprotonated 6-(1,1-dimethylbenzyl)-2,2′-bipyridine, to assess the chance of tracking their subcellular distribution by atomic force microscopy (AFM), and surface enhanced Raman spectroscopy (SERS) techniques. An extensive physicochemical characterization of the formed conjugates was, thus, carried out by applying a variety of methods (density functional theory—DFT, UV/Vis spectrophotometry, AFM, Raman spectroscopy, and SERS). The resulting gold(III) complexes/AuNPs conjugates turned out to be pretty stable. Interestingly, they exhibited a dramatically increased resonance intensity in the Raman spectra induced by AuNPs. For testing the use of the functionalized AuNPs for biosensing, their distribution in the nuclear, cytosolic, and membrane cell fractions obtained from human lymphocytes was investigated by AFM and SERS. The conjugates were detected in the membrane and nuclear cell fractions but not in the cytosol. The AFM method confirmed that conjugates induced changes in the morphology and nanostructure of the membrane and nuclear fractions. The obtained results point out that the conjugates formed between AuNPs and gold(III) complexes may be used as a tool for tracking metallodrug distribution in the different cell fractions.",
journal = "International Journal of Molecular Sciences",
title = "Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue",
volume = "20",
number = "24",
pages = "6306",
doi = "10.3390/ijms20246306"
}
Bondžić, A. M., Leskovac, A., Petrović, S., Vasić Anićijević, D. D., Luce, M., Massai, L., Generosi, A., Paci, B., Cricenti, A., Messori, L.,& Vasić, V. M.. (2019). Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue. in International Journal of Molecular Sciences, 20(24), 6306.
https://doi.org/10.3390/ijms20246306
Bondžić AM, Leskovac A, Petrović S, Vasić Anićijević DD, Luce M, Massai L, Generosi A, Paci B, Cricenti A, Messori L, Vasić VM. Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue. in International Journal of Molecular Sciences. 2019;20(24):6306.
doi:10.3390/ijms20246306 .
Bondžić, Aleksandra M., Leskovac, Andreja, Petrović, Sandra, Vasić Anićijević, Dragana D., Luce, Marco, Massai, Lara, Generosi, Amanda, Paci, Barbara, Cricenti, Antonio, Messori, Luigi, Vasić, Vesna M., "Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue" in International Journal of Molecular Sciences, 20, no. 24 (2019):6306,
https://doi.org/10.3390/ijms20246306 . .
3
1
3

Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles

Bondžić, Aleksandra M.; Vujačić Nikezić, Ana V.; Klekotka, Urszula; Marković, Mirjana; Vodnik, Vesna; Kalska, B.; Vasić, Vesna M.

(2019)

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Vujačić Nikezić, Ana V.
AU  - Klekotka,  Urszula
AU  - Marković, Mirjana
AU  - Vodnik, Vesna
AU  - Kalska, B.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8812
AB  - Abstract: This paper presents the study of the interaction between gold nanoparticles (AuNPs) and antitumor gold complexes, [Au(OH)2(bipy)][PF6], [Au(CH3COO)2(pydmb-H)], and [Au(bipydmb-H)(OH)][PF6], in order to estimate the possibility for metal complex tracking in cells using nanospectroscopy approach. Decrease of intensity of the surface plasmon absorption band at 524 nm and the appearance of a new broad band at ∼640 nm, followed by their red shift were observed in the presence of the complexes. TEM images showed that the average size and shape of AuNPs did not change after the addition of gold complexes. DLS and zeta potential measurements pointed out to the metal complex adsorption on the surface of AuNPs followed by flocculation process. In addition, kinetic studies indicated strong bond formation between these complexes and AuNPs. Accordingly, AuNPs/complex conjugates have the potential to be applied for the tracking of these metal complexes in the cells using nanospectroscopy approach. © 2019, Pleiades Publishing, Ltd.
T2  - Russian Journal of Physical Chemistry A
T1  - Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles
VL  - 93
IS  - 13
SP  - 2765
EP  - 2770
DO  - 10.1134/S0036024419130065
ER  - 
@article{
author = "Bondžić, Aleksandra M. and Vujačić Nikezić, Ana V. and Klekotka,  Urszula and Marković, Mirjana and Vodnik, Vesna and Kalska, B. and Vasić, Vesna M.",
year = "2019",
abstract = "Abstract: This paper presents the study of the interaction between gold nanoparticles (AuNPs) and antitumor gold complexes, [Au(OH)2(bipy)][PF6], [Au(CH3COO)2(pydmb-H)], and [Au(bipydmb-H)(OH)][PF6], in order to estimate the possibility for metal complex tracking in cells using nanospectroscopy approach. Decrease of intensity of the surface plasmon absorption band at 524 nm and the appearance of a new broad band at ∼640 nm, followed by their red shift were observed in the presence of the complexes. TEM images showed that the average size and shape of AuNPs did not change after the addition of gold complexes. DLS and zeta potential measurements pointed out to the metal complex adsorption on the surface of AuNPs followed by flocculation process. In addition, kinetic studies indicated strong bond formation between these complexes and AuNPs. Accordingly, AuNPs/complex conjugates have the potential to be applied for the tracking of these metal complexes in the cells using nanospectroscopy approach. © 2019, Pleiades Publishing, Ltd.",
journal = "Russian Journal of Physical Chemistry A",
title = "Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles",
volume = "93",
number = "13",
pages = "2765-2770",
doi = "10.1134/S0036024419130065"
}
Bondžić, A. M., Vujačić Nikezić, A. V., Klekotka, U., Marković, M., Vodnik, V., Kalska, B.,& Vasić, V. M.. (2019). Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles. in Russian Journal of Physical Chemistry A, 93(13), 2765-2770.
https://doi.org/10.1134/S0036024419130065
Bondžić AM, Vujačić Nikezić AV, Klekotka U, Marković M, Vodnik V, Kalska B, Vasić VM. Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles. in Russian Journal of Physical Chemistry A. 2019;93(13):2765-2770.
doi:10.1134/S0036024419130065 .
Bondžić, Aleksandra M., Vujačić Nikezić, Ana V., Klekotka,  Urszula, Marković, Mirjana, Vodnik, Vesna, Kalska, B., Vasić, Vesna M., "Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles" in Russian Journal of Physical Chemistry A, 93, no. 13 (2019):2765-2770,
https://doi.org/10.1134/S0036024419130065 . .
1
1
2

Towards better quality criteria of European honeydew honey: Phenolic profile and antioxidant capacity

Vasić, Vesna M.; Gašić, Uroš M.; Stanković, Dalibor M.; Lušić, Dražen; Vukić-Lušić, Darija; Milojković-Opsenica, Dušanka M.; Tešić, Živoslav; Trifković, Jelena Đ.

(2019)

TY  - JOUR
AU  - Vasić, Vesna M.
AU  - Gašić, Uroš M.
AU  - Stanković, Dalibor M.
AU  - Lušić, Dražen
AU  - Vukić-Lušić, Darija
AU  - Milojković-Opsenica, Dušanka M.
AU  - Tešić, Živoslav
AU  - Trifković, Jelena Đ.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618316169
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7868
AB  - Concerning the particular nutritive value of honeydew honey compared to blossom honey, and small number of studies defining botanical origin of honeydew honey, comprehensive analysis of phenolic profile of 64 honeydew honey samples of specific botanical origin was performed. Two advanced techniques of liquid chromatography hyphenated with mass spectrometry were used for identification of a total of 52 compounds and quantification of 25 of them. Pattern recognition analysis applied on data on phenolic compounds content confirmed that quercetin, naringenin, caffeoylquinic acid, hydroxyphenylacetic acid, apigenin and genistein, could be considered as potential markers of botanical origin of honeydew honey. Spectroscopic and electrochemical approaches were applied for the evaluation of the antioxidant capacity. Quercus sps. samples, Quercus frainetto and Quercus ilex, showed high biological activity and specific chemical composition. Additionally, cyclic voltammetry profiles were used for characterization and natural clustering of honeydew honey for the first time. © 2018 Elsevier Ltd
T2  - Food Chemistry
T1  - Towards better quality criteria of European honeydew honey: Phenolic profile and antioxidant capacity
VL  - 274
SP  - 629
EP  - 641
DO  - 10.1016/j.foodchem.2018.09.045
ER  - 
@article{
author = "Vasić, Vesna M. and Gašić, Uroš M. and Stanković, Dalibor M. and Lušić, Dražen and Vukić-Lušić, Darija and Milojković-Opsenica, Dušanka M. and Tešić, Živoslav and Trifković, Jelena Đ.",
year = "2019",
abstract = "Concerning the particular nutritive value of honeydew honey compared to blossom honey, and small number of studies defining botanical origin of honeydew honey, comprehensive analysis of phenolic profile of 64 honeydew honey samples of specific botanical origin was performed. Two advanced techniques of liquid chromatography hyphenated with mass spectrometry were used for identification of a total of 52 compounds and quantification of 25 of them. Pattern recognition analysis applied on data on phenolic compounds content confirmed that quercetin, naringenin, caffeoylquinic acid, hydroxyphenylacetic acid, apigenin and genistein, could be considered as potential markers of botanical origin of honeydew honey. Spectroscopic and electrochemical approaches were applied for the evaluation of the antioxidant capacity. Quercus sps. samples, Quercus frainetto and Quercus ilex, showed high biological activity and specific chemical composition. Additionally, cyclic voltammetry profiles were used for characterization and natural clustering of honeydew honey for the first time. © 2018 Elsevier Ltd",
journal = "Food Chemistry",
title = "Towards better quality criteria of European honeydew honey: Phenolic profile and antioxidant capacity",
volume = "274",
pages = "629-641",
doi = "10.1016/j.foodchem.2018.09.045"
}
Vasić, V. M., Gašić, U. M., Stanković, D. M., Lušić, D., Vukić-Lušić, D., Milojković-Opsenica, D. M., Tešić, Ž.,& Trifković, J. Đ.. (2019). Towards better quality criteria of European honeydew honey: Phenolic profile and antioxidant capacity. in Food Chemistry, 274, 629-641.
https://doi.org/10.1016/j.foodchem.2018.09.045
Vasić VM, Gašić UM, Stanković DM, Lušić D, Vukić-Lušić D, Milojković-Opsenica DM, Tešić Ž, Trifković JĐ. Towards better quality criteria of European honeydew honey: Phenolic profile and antioxidant capacity. in Food Chemistry. 2019;274:629-641.
doi:10.1016/j.foodchem.2018.09.045 .
Vasić, Vesna M., Gašić, Uroš M., Stanković, Dalibor M., Lušić, Dražen, Vukić-Lušić, Darija, Milojković-Opsenica, Dušanka M., Tešić, Živoslav, Trifković, Jelena Đ., "Towards better quality criteria of European honeydew honey: Phenolic profile and antioxidant capacity" in Food Chemistry, 274 (2019):629-641,
https://doi.org/10.1016/j.foodchem.2018.09.045 . .
1
41
35
38

UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations

Savić, Jasmina; Petrović, Sandra; Leskovac, Andreja; Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Tanović, Brankica B.; Gašić, Slavica M.; Vasić, Vesna M.

(2019)

TY  - JOUR
AU  - Savić, Jasmina
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Tanović, Brankica B.
AU  - Gašić, Slavica M.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7821
AB  - UV-C irradiation is widely used in the food industry. However, the health effects from dietary exposure to the irradiated pesticide residues retained in foodstuffs are underestimated. In this study, technical chlorpyrifos (TCPF) and its oil in water (EW) and emulsifiable concentrate (EC) formulations were irradiated by UV-C, and their photodegradation products were subjected to toxicity assessment, including determination of acetylcholinesterase (AChE) activity, genotoxicity and oxidative stress using human blood cells as a model system. Toxicity studies were performed using the chlorpyrifos concentrations in the range of those proposed as the maximum residue levels in plant commodities. TCPF, EW and EC photodegradation products induced DNA damage and oxidative stress, and their genotoxicity did not decrease as a function of irradiation time. Irradiated TCPF and EC are more potent AChE inhibitors than irradiated EW. Accordingly, the application of UV-C irradiation must be considered when processing the plants previously treated with chlorpyrifos formulations. © 2018 Elsevier Ltd
T2  - Food Chemistry
T1  - UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations
VL  - 271
SP  - 469
EP  - 478
DO  - 10.1016/j.foodchem.2018.07.207
ER  - 
@article{
author = "Savić, Jasmina and Petrović, Sandra and Leskovac, Andreja and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Tanović, Brankica B. and Gašić, Slavica M. and Vasić, Vesna M.",
year = "2019",
abstract = "UV-C irradiation is widely used in the food industry. However, the health effects from dietary exposure to the irradiated pesticide residues retained in foodstuffs are underestimated. In this study, technical chlorpyrifos (TCPF) and its oil in water (EW) and emulsifiable concentrate (EC) formulations were irradiated by UV-C, and their photodegradation products were subjected to toxicity assessment, including determination of acetylcholinesterase (AChE) activity, genotoxicity and oxidative stress using human blood cells as a model system. Toxicity studies were performed using the chlorpyrifos concentrations in the range of those proposed as the maximum residue levels in plant commodities. TCPF, EW and EC photodegradation products induced DNA damage and oxidative stress, and their genotoxicity did not decrease as a function of irradiation time. Irradiated TCPF and EC are more potent AChE inhibitors than irradiated EW. Accordingly, the application of UV-C irradiation must be considered when processing the plants previously treated with chlorpyrifos formulations. © 2018 Elsevier Ltd",
journal = "Food Chemistry",
title = "UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations",
volume = "271",
pages = "469-478",
doi = "10.1016/j.foodchem.2018.07.207"
}
Savić, J., Petrović, S., Leskovac, A., Lazarević-Pašti, T., Nastasijević, B. J., Tanović, B. B., Gašić, S. M.,& Vasić, V. M.. (2019). UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry, 271, 469-478.
https://doi.org/10.1016/j.foodchem.2018.07.207
Savić J, Petrović S, Leskovac A, Lazarević-Pašti T, Nastasijević BJ, Tanović BB, Gašić SM, Vasić VM. UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry. 2019;271:469-478.
doi:10.1016/j.foodchem.2018.07.207 .
Savić, Jasmina, Petrović, Sandra, Leskovac, Andreja, Lazarević-Pašti, Tamara, Nastasijević, Branislav J., Tanović, Brankica B., Gašić, Slavica M., Vasić, Vesna M., "UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations" in Food Chemistry, 271 (2019):469-478,
https://doi.org/10.1016/j.foodchem.2018.07.207 . .
11
8

Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations

Savić, Jasmina; Petrović, Sandra; Leskovac, Andreja; Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Tanović, Brankica B.; Gašić, Slavica M.; Vasić, Vesna M.

(2019)

TY  - GEN
AU  - Savić, Jasmina
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Tanović, Brankica B.
AU  - Gašić, Slavica M.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7831
AB  - Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; 
Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; 
Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4
T2  - Food Chemistry
T1  - Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations
VL  - 271
SP  - 469
EP  - 478
DO  - 10.1016/j.foodchem.2018.07.207
ER  - 
@misc{
author = "Savić, Jasmina and Petrović, Sandra and Leskovac, Andreja and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Tanović, Brankica B. and Gašić, Slavica M. and Vasić, Vesna M.",
year = "2019",
abstract = "Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; 
Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; 
Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4",
journal = "Food Chemistry",
title = "Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations",
volume = "271",
pages = "469-478",
doi = "10.1016/j.foodchem.2018.07.207"
}
Savić, J., Petrović, S., Leskovac, A., Lazarević-Pašti, T., Nastasijević, B. J., Tanović, B. B., Gašić, S. M.,& Vasić, V. M.. (2019). Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry, 271, 469-478.
https://doi.org/10.1016/j.foodchem.2018.07.207
Savić J, Petrović S, Leskovac A, Lazarević-Pašti T, Nastasijević BJ, Tanović BB, Gašić SM, Vasić VM. Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry. 2019;271:469-478.
doi:10.1016/j.foodchem.2018.07.207 .
Savić, Jasmina, Petrović, Sandra, Leskovac, Andreja, Lazarević-Pašti, Tamara, Nastasijević, Branislav J., Tanović, Brankica B., Gašić, Slavica M., Vasić, Vesna M., "Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations" in Food Chemistry, 271 (2019):469-478,
https://doi.org/10.1016/j.foodchem.2018.07.207 . .
11
9

Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models

Marković, Tatjana; Radanović, Dragoja; Nastasijević, Branislav J.; Antić-Mladenović, Svetlana; Vasić, Vesna M.; Matković, Ana

(2019)

TY  - JOUR
AU  - Marković, Tatjana
AU  - Radanović, Dragoja
AU  - Nastasijević, Branislav J.
AU  - Antić-Mladenović, Svetlana
AU  - Vasić, Vesna M.
AU  - Matković, Ana
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669019301190
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8073
AB  - Yellow gentian (Gentiana lutea ssp. symphyandra (Murb.) Hayek) is a high-mountainous perennial spontaneously growing on meadows and open slopes of the Eastern part of the Alps and the Balkan Peninsula. While the excessive exploitation endangered its survival in the nature in many European countries, the orientation to its large-scale production enables its protection at natural stands and satisfaction of increasing market demands for its roots, a highly-valuable herbal drug (Gentianae radix). During the six-year field trial established with Yellow gentian nursery plants on black water-air permeable and biodegradable film, in dry farming conditions of Mountain Tara (Serbia), the influence of a sigle basal application of farm yard manure and mineral fertilizer, at different planting densities (11.1, 13.3 and 16 plants m −2 ) on produced yield (roots), has been investigated. Single dose fertilization positively influenced the yields but did not provide optimal supplies for cultivated crop in the second part of experiment. It depended on crop age, planting density but also the climatic conditions. In the second part of plant production period, particulary in denser establishemnts, additional fertilization should be applied. Providing the appropriate amounts of fertilizers, Yellow gentian can be successfully grown even in the densest planting model (16 plants m −2 ), which is quite important in respect to the economic feasibility of cultivation. Similar yields achieved in mineral and organic treatments (4.51 kg m −2 and 4.85 kg m -2 of fresh root, respectively), suggest the roots might be successfully produced in both, conventional and organic cultivation model. In attempt to estimate impact of fertilization treatments on chemical quality of produced raw material (Gentianae radix) the content of several pharmacological constituents (loganic acid, swertiamarin, gentiopicroside, sweroside, amarogentin and isogentisin) was evaluated; gentiopicroside and loganic acid were the most dominant ones, regadless the fertilization, whereas the content of isogentisin was significantly increased in both, organic and mineral fertilization model, and content of sweroside, only in mineral model. With regard to the safety of produced raw material, the contents of biogenic (Fe, Mn, Zn, Cu) and non-biogenic (Pb) trace elements were analyzed; all trace elements were within the safty limits except the Cd content which slightly exceeded the limit. © 2019 Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models
VL  - 132
SP  - 236
EP  - 244
DO  - 10.1016/j.indcrop.2019.02.027
ER  - 
@article{
author = "Marković, Tatjana and Radanović, Dragoja and Nastasijević, Branislav J. and Antić-Mladenović, Svetlana and Vasić, Vesna M. and Matković, Ana",
year = "2019",
abstract = "Yellow gentian (Gentiana lutea ssp. symphyandra (Murb.) Hayek) is a high-mountainous perennial spontaneously growing on meadows and open slopes of the Eastern part of the Alps and the Balkan Peninsula. While the excessive exploitation endangered its survival in the nature in many European countries, the orientation to its large-scale production enables its protection at natural stands and satisfaction of increasing market demands for its roots, a highly-valuable herbal drug (Gentianae radix). During the six-year field trial established with Yellow gentian nursery plants on black water-air permeable and biodegradable film, in dry farming conditions of Mountain Tara (Serbia), the influence of a sigle basal application of farm yard manure and mineral fertilizer, at different planting densities (11.1, 13.3 and 16 plants m −2 ) on produced yield (roots), has been investigated. Single dose fertilization positively influenced the yields but did not provide optimal supplies for cultivated crop in the second part of experiment. It depended on crop age, planting density but also the climatic conditions. In the second part of plant production period, particulary in denser establishemnts, additional fertilization should be applied. Providing the appropriate amounts of fertilizers, Yellow gentian can be successfully grown even in the densest planting model (16 plants m −2 ), which is quite important in respect to the economic feasibility of cultivation. Similar yields achieved in mineral and organic treatments (4.51 kg m −2 and 4.85 kg m -2 of fresh root, respectively), suggest the roots might be successfully produced in both, conventional and organic cultivation model. In attempt to estimate impact of fertilization treatments on chemical quality of produced raw material (Gentianae radix) the content of several pharmacological constituents (loganic acid, swertiamarin, gentiopicroside, sweroside, amarogentin and isogentisin) was evaluated; gentiopicroside and loganic acid were the most dominant ones, regadless the fertilization, whereas the content of isogentisin was significantly increased in both, organic and mineral fertilization model, and content of sweroside, only in mineral model. With regard to the safety of produced raw material, the contents of biogenic (Fe, Mn, Zn, Cu) and non-biogenic (Pb) trace elements were analyzed; all trace elements were within the safty limits except the Cd content which slightly exceeded the limit. © 2019 Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models",
volume = "132",
pages = "236-244",
doi = "10.1016/j.indcrop.2019.02.027"
}
Marković, T., Radanović, D., Nastasijević, B. J., Antić-Mladenović, S., Vasić, V. M.,& Matković, A.. (2019). Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models. in Industrial Crops and Products, 132, 236-244.
https://doi.org/10.1016/j.indcrop.2019.02.027
Marković T, Radanović D, Nastasijević BJ, Antić-Mladenović S, Vasić VM, Matković A. Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models. in Industrial Crops and Products. 2019;132:236-244.
doi:10.1016/j.indcrop.2019.02.027 .
Marković, Tatjana, Radanović, Dragoja, Nastasijević, Branislav J., Antić-Mladenović, Svetlana, Vasić, Vesna M., Matković, Ana, "Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models" in Industrial Crops and Products, 132 (2019):236-244,
https://doi.org/10.1016/j.indcrop.2019.02.027 . .
5
5
5

The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water

Lazarević-Pašti, Tamara; Anićijević, Vladan J.; Baljozović, Miloš; Vasić Anićijević, Dragana D.; Gutić, Sanjin J.; Vasić, Vesna M.; Skorodumova, Natalia V.; Pašti, Igor A.

(2018)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Anićijević, Vladan J.
AU  - Baljozović, Miloš
AU  - Vasić Anićijević, Dragana D.
AU  - Gutić, Sanjin J.
AU  - Vasić, Vesna M.
AU  - Skorodumova, Natalia V.
AU  - Pašti, Igor A.
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C8EN00171E
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7730
AB  - The wide use of pesticides in modern agriculture and other areas results in an urgent need for their efficient removal from the environment. Adsorption of pesticides is one of the most commonly used strategies for this task. Here we analyze the adsorption of two organophosphorus pesticides, dimethoate (DMT) and chlorpyrifos (CPF), on graphene-based materials. The adsorption was found to be very sensitive to the structure of the adsorbents used. In particular, aliphatic DMT was found to prefer hydrophilic oxidized graphene surfaces. The CPF molecule, which contains an aromatic moiety, prefers adsorption on the surface of a graphene basal plane with high structural order and preserved π electron system. The toxicity of pesticide solutions is reduced after adsorption, suggesting that there is no oxidation of DMT and CPF to more toxic oxo forms. We emphasize that the combination of structural properties of adsorbents and adsorbates defines the adsorption of organophosphorus pesticides on graphene-based materials, while the specific surface area of adsorbents is not the major factor.
T2  - Environmental Science: Nano
T1  - The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water
VL  - 5
IS  - 6
SP  - 1482
EP  - 1494
DO  - 10.1039/C8EN00171E
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Anićijević, Vladan J. and Baljozović, Miloš and Vasić Anićijević, Dragana D. and Gutić, Sanjin J. and Vasić, Vesna M. and Skorodumova, Natalia V. and Pašti, Igor A.",
year = "2018",
abstract = "The wide use of pesticides in modern agriculture and other areas results in an urgent need for their efficient removal from the environment. Adsorption of pesticides is one of the most commonly used strategies for this task. Here we analyze the adsorption of two organophosphorus pesticides, dimethoate (DMT) and chlorpyrifos (CPF), on graphene-based materials. The adsorption was found to be very sensitive to the structure of the adsorbents used. In particular, aliphatic DMT was found to prefer hydrophilic oxidized graphene surfaces. The CPF molecule, which contains an aromatic moiety, prefers adsorption on the surface of a graphene basal plane with high structural order and preserved π electron system. The toxicity of pesticide solutions is reduced after adsorption, suggesting that there is no oxidation of DMT and CPF to more toxic oxo forms. We emphasize that the combination of structural properties of adsorbents and adsorbates defines the adsorption of organophosphorus pesticides on graphene-based materials, while the specific surface area of adsorbents is not the major factor.",
journal = "Environmental Science: Nano",
title = "The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water",
volume = "5",
number = "6",
pages = "1482-1494",
doi = "10.1039/C8EN00171E"
}
Lazarević-Pašti, T., Anićijević, V. J., Baljozović, M., Vasić Anićijević, D. D., Gutić, S. J., Vasić, V. M., Skorodumova, N. V.,& Pašti, I. A.. (2018). The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water. in Environmental Science: Nano, 5(6), 1482-1494.
https://doi.org/10.1039/C8EN00171E
Lazarević-Pašti T, Anićijević VJ, Baljozović M, Vasić Anićijević DD, Gutić SJ, Vasić VM, Skorodumova NV, Pašti IA. The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water. in Environmental Science: Nano. 2018;5(6):1482-1494.
doi:10.1039/C8EN00171E .
Lazarević-Pašti, Tamara, Anićijević, Vladan J., Baljozović, Miloš, Vasić Anićijević, Dragana D., Gutić, Sanjin J., Vasić, Vesna M., Skorodumova, Natalia V., Pašti, Igor A., "The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water" in Environmental Science: Nano, 5, no. 6 (2018):1482-1494,
https://doi.org/10.1039/C8EN00171E . .
7
40
31
39

Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites

Vujačić Nikezić, Ana V.; Janjić, Goran V.; Bondžić, Aleksandra M.; Zarić, Božidarka; Vasić Anićijević, Dragana D.; Momić, Tatjana; Vasić, Vesna M.

(2018)

TY  - JOUR
AU  - Vujačić Nikezić, Ana V.
AU  - Janjić, Goran V.
AU  - Bondžić, Aleksandra M.
AU  - Zarić, Božidarka
AU  - Vasić Anićijević, Dragana D.
AU  - Momić, Tatjana
AU  - Vasić, Vesna M.
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C8MT00111A
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7812
AB  - The present paper deals with investigation of the interaction between selected simple structure Au(iii) ([AuCl4]-, [AuCl2(dmso)2]+, [AuCl2(bipy)]+) and Pt(ii) ([PtCl2(dmso)2]) complexes with Na/K-ATPase as the target enzyme, using an experimental and theoretical approach. Reaction stoichiometries and binding constants for these enzyme/complex systems were determined, while kinetic measurements were used in order to reveal the type of inhibition. Based on the results obtained by quantum mechanical calculations (electrostatic surface potential (ESP), volume and surface of the complexes) the nature of the investigated complexes was characterized. By using the solvent accessible surface area (SASA) applied on specific inhibitory sites (ion channel and intracellular domains) the nature of these sites was described. Docking studies were used to determine the theoretical probability of the non-covalent metal binding site positions. Inhibition studies implied that all the investigated complexes decreased the activity of the enzyme while the kinetic analysis indicated an uncompetitive mode of inhibition for the selected complexes. Docking results suggested that the main inhibitory site of all these complexes is located in the ion translocation pathway on the extracellular side in the E2P enzyme conformation, similar to the case of cardiac glycosides, specific Na/K-ATPase inhibitors. Also, based on our knowledge, the hydrolyzed forms of [AuCl4]- and [PtCl2(dmso)2] complexes were investigated for the first time by theoretical calculations in this paper. Thereby, a new inhibitory site situated between the M2 and M4 helices was revealed. Binding in this site induces conformational changes in the enzyme domains and perturbs the E1-E2P conformational equilibrium, causing enzyme inhibition.
T2  - Metallomics
T1  - Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites
VL  - 10
IS  - 7
SP  - 1003
EP  - 1015
DO  - 10.1039/C8MT00111A
ER  - 
@article{
author = "Vujačić Nikezić, Ana V. and Janjić, Goran V. and Bondžić, Aleksandra M. and Zarić, Božidarka and Vasić Anićijević, Dragana D. and Momić, Tatjana and Vasić, Vesna M.",
year = "2018",
abstract = "The present paper deals with investigation of the interaction between selected simple structure Au(iii) ([AuCl4]-, [AuCl2(dmso)2]+, [AuCl2(bipy)]+) and Pt(ii) ([PtCl2(dmso)2]) complexes with Na/K-ATPase as the target enzyme, using an experimental and theoretical approach. Reaction stoichiometries and binding constants for these enzyme/complex systems were determined, while kinetic measurements were used in order to reveal the type of inhibition. Based on the results obtained by quantum mechanical calculations (electrostatic surface potential (ESP), volume and surface of the complexes) the nature of the investigated complexes was characterized. By using the solvent accessible surface area (SASA) applied on specific inhibitory sites (ion channel and intracellular domains) the nature of these sites was described. Docking studies were used to determine the theoretical probability of the non-covalent metal binding site positions. Inhibition studies implied that all the investigated complexes decreased the activity of the enzyme while the kinetic analysis indicated an uncompetitive mode of inhibition for the selected complexes. Docking results suggested that the main inhibitory site of all these complexes is located in the ion translocation pathway on the extracellular side in the E2P enzyme conformation, similar to the case of cardiac glycosides, specific Na/K-ATPase inhibitors. Also, based on our knowledge, the hydrolyzed forms of [AuCl4]- and [PtCl2(dmso)2] complexes were investigated for the first time by theoretical calculations in this paper. Thereby, a new inhibitory site situated between the M2 and M4 helices was revealed. Binding in this site induces conformational changes in the enzyme domains and perturbs the E1-E2P conformational equilibrium, causing enzyme inhibition.",
journal = "Metallomics",
title = "Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites",
volume = "10",
number = "7",
pages = "1003-1015",
doi = "10.1039/C8MT00111A"
}
Vujačić Nikezić, A. V., Janjić, G. V., Bondžić, A. M., Zarić, B., Vasić Anićijević, D. D., Momić, T.,& Vasić, V. M.. (2018). Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites. in Metallomics, 10(7), 1003-1015.
https://doi.org/10.1039/C8MT00111A
Vujačić Nikezić AV, Janjić GV, Bondžić AM, Zarić B, Vasić Anićijević DD, Momić T, Vasić VM. Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites. in Metallomics. 2018;10(7):1003-1015.
doi:10.1039/C8MT00111A .
Vujačić Nikezić, Ana V., Janjić, Goran V., Bondžić, Aleksandra M., Zarić, Božidarka, Vasić Anićijević, Dragana D., Momić, Tatjana, Vasić, Vesna M., "Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites" in Metallomics, 10, no. 7 (2018):1003-1015,
https://doi.org/10.1039/C8MT00111A . .
2
3
3

Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals

Čolović, Mirjana B.; Vasić, Vesna M.; Đurić, Dragan M.; Krstić, Danijela Z.

(2018)

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1926
AB  - Background: Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in protein structure. Sulphur amino acids are involved in the synthesis of intracellular antioxidants such as glutathione and N-acetyl cysteine. Moreover, naturally occurring sulphur-containing ligands are effective and safe detoxifying agents, often used in order to prevent toxic metal ions effects and their accumulation in human body. Methods: Literature search for peer-reviewed articles was performed using PubMed and Scopus databases, and utilizing appropriate keywords. Results: This review is focused on sulphur-containing amino acids - methionine, cysteine, taurine, and their derivatives - glutathione and N-acetylcysteine, and their defense effects as antioxidant agents against free radicals. Additionally, the protective effects of sulphur-containing ligands against the toxic effects of heavy and transition metal ions, and their reactivation role towards the enzyme inhibition are described. Conclusion: Sulphur-containing amino acids represent a powerful part of cell antioxidant system. Thus, they are essential in the maintenance of normal cellular functions and health. In addition to their worthy antioxidant action, sulphur-containing amino acids may offer a chelating site for heavy metals. Accordingly, they may be supplemented during chelating therapy, providing beneficial effects in eliminating toxic metals.
T2  - Current Medicinal Chemistry
T1  - Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals
VL  - 25
IS  - 3
SP  - 324
EP  - 335
DO  - 10.2174/0929867324666170609075434
ER  - 
@article{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2018",
abstract = "Background: Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in protein structure. Sulphur amino acids are involved in the synthesis of intracellular antioxidants such as glutathione and N-acetyl cysteine. Moreover, naturally occurring sulphur-containing ligands are effective and safe detoxifying agents, often used in order to prevent toxic metal ions effects and their accumulation in human body. Methods: Literature search for peer-reviewed articles was performed using PubMed and Scopus databases, and utilizing appropriate keywords. Results: This review is focused on sulphur-containing amino acids - methionine, cysteine, taurine, and their derivatives - glutathione and N-acetylcysteine, and their defense effects as antioxidant agents against free radicals. Additionally, the protective effects of sulphur-containing ligands against the toxic effects of heavy and transition metal ions, and their reactivation role towards the enzyme inhibition are described. Conclusion: Sulphur-containing amino acids represent a powerful part of cell antioxidant system. Thus, they are essential in the maintenance of normal cellular functions and health. In addition to their worthy antioxidant action, sulphur-containing amino acids may offer a chelating site for heavy metals. Accordingly, they may be supplemented during chelating therapy, providing beneficial effects in eliminating toxic metals.",
journal = "Current Medicinal Chemistry",
title = "Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals",
volume = "25",
number = "3",
pages = "324-335",
doi = "10.2174/0929867324666170609075434"
}
Čolović, M. B., Vasić, V. M., Đurić, D. M.,& Krstić, D. Z.. (2018). Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals. in Current Medicinal Chemistry, 25(3), 324-335.
https://doi.org/10.2174/0929867324666170609075434
Čolović MB, Vasić VM, Đurić DM, Krstić DZ. Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals. in Current Medicinal Chemistry. 2018;25(3):324-335.
doi:10.2174/0929867324666170609075434 .
Čolović, Mirjana B., Vasić, Vesna M., Đurić, Dragan M., Krstić, Danijela Z., "Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals" in Current Medicinal Chemistry, 25, no. 3 (2018):324-335,
https://doi.org/10.2174/0929867324666170609075434 . .
53
43
50

Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents

Leskovac, Andreja; Petrović, Sandra; Lazarević-Pašti, Tamara; Krstić, Milena P.; Vasić, Vesna M.

(2018)

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Lazarević-Pašti, Tamara
AU  - Krstić, Milena P.
AU  - Vasić, Vesna M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s00775-018-1560-x
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7765
AB  - In recent years, the search for effective anticancer compounds based on transition metal complexes has been the focus of medical investigations. The synergy between the ruthenium(II) and N-alkylphenothiazine counter-ions (chlorpromazine hydrochloride, thioridazine hydrochloride and trifluoperazine dihydrochloride, respectively) through the formation of three different complexes (1–3) was investigated. We explored whether the selected counter-ions and complexes might affect redox homeostasis and genome integrity of normal human blood cells, and induce an inhibition of Na+/K+-ATPase and AChE at pharmacologically relevant doses. Our results have shown that counter-ions and complexes did not affect the activity of Na+/K+-ATPase, while AChE activity was inhibited in a dose-dependent manner. All investigated compounds disturbed the viability and redox homeostasis of lymphocytes. Complexes 1 and 2 displayed potent cytotoxic and prooxidant action while complex 3 behaved as a weaker genotoxic inducer. Still, the tested complexes appeared to be less genotoxic and more cytostatic than the corresponding counter-ions. The effects of selected complexes were also tested in PC12 and U2OS cancer cells with special attention being given to the ability of phenothiazines to affect dopamine D2 receptors. Using the confocal laser scanning microscopy, we observed that all the complexes reduced cell viability. Although all investigated complexes have been bound to the dopamine receptor D2-eGFP, only complex 3 reduced its surface density and increased its lateral mobility in investigated cell lines. Albeit the role of alternative targets for complex 3 cannot be ruled out, its effects should be further examined as potential treatment strategy against cancer cells that overexpress D2.
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents
VL  - 23
IS  - 5
SP  - 689
EP  - 704
DO  - 10.1007/s00775-018-1560-x
ER  - 
@article{
author = "Leskovac, Andreja and Petrović, Sandra and Lazarević-Pašti, Tamara and Krstić, Milena P. and Vasić, Vesna M.",
year = "2018",
abstract = "In recent years, the search for effective anticancer compounds based on transition metal complexes has been the focus of medical investigations. The synergy between the ruthenium(II) and N-alkylphenothiazine counter-ions (chlorpromazine hydrochloride, thioridazine hydrochloride and trifluoperazine dihydrochloride, respectively) through the formation of three different complexes (1–3) was investigated. We explored whether the selected counter-ions and complexes might affect redox homeostasis and genome integrity of normal human blood cells, and induce an inhibition of Na+/K+-ATPase and AChE at pharmacologically relevant doses. Our results have shown that counter-ions and complexes did not affect the activity of Na+/K+-ATPase, while AChE activity was inhibited in a dose-dependent manner. All investigated compounds disturbed the viability and redox homeostasis of lymphocytes. Complexes 1 and 2 displayed potent cytotoxic and prooxidant action while complex 3 behaved as a weaker genotoxic inducer. Still, the tested complexes appeared to be less genotoxic and more cytostatic than the corresponding counter-ions. The effects of selected complexes were also tested in PC12 and U2OS cancer cells with special attention being given to the ability of phenothiazines to affect dopamine D2 receptors. Using the confocal laser scanning microscopy, we observed that all the complexes reduced cell viability. Although all investigated complexes have been bound to the dopamine receptor D2-eGFP, only complex 3 reduced its surface density and increased its lateral mobility in investigated cell lines. Albeit the role of alternative targets for complex 3 cannot be ruled out, its effects should be further examined as potential treatment strategy against cancer cells that overexpress D2.",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents",
volume = "23",
number = "5",
pages = "689-704",
doi = "10.1007/s00775-018-1560-x"
}
Leskovac, A., Petrović, S., Lazarević-Pašti, T., Krstić, M. P.,& Vasić, V. M.. (2018). Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents. in JBIC Journal of Biological Inorganic Chemistry, 23(5), 689-704.
https://doi.org/10.1007/s00775-018-1560-x
Leskovac A, Petrović S, Lazarević-Pašti T, Krstić MP, Vasić VM. Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents. in JBIC Journal of Biological Inorganic Chemistry. 2018;23(5):689-704.
doi:10.1007/s00775-018-1560-x .
Leskovac, Andreja, Petrović, Sandra, Lazarević-Pašti, Tamara, Krstić, Milena P., Vasić, Vesna M., "Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents" in JBIC Journal of Biological Inorganic Chemistry, 23, no. 5 (2018):689-704,
https://doi.org/10.1007/s00775-018-1560-x . .
5
3
2

Impact of vinification procedure on fruit wine inhibitory activity against α-glucosidase

Čakar, Uroš; Grozdanić, Nađa; Pejin, Boris; Vasić, Vesna M.; Čakar, Mira M.; Petrović, Aleksandar V.; Đorđević, Brižita

(2018)

TY  - JOUR
AU  - Čakar, Uroš
AU  - Grozdanić, Nađa
AU  - Pejin, Boris
AU  - Vasić, Vesna M.
AU  - Čakar, Mira M.
AU  - Petrović, Aleksandar V.
AU  - Đorđević, Brižita
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2212429218301512
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7772
AB  - α-Glucosidase inhibitory activity (AGL) of fruit wine samples made from blueberry, black chokeberry, blackberry, raspberry and sour cherry cultivars grown in Serbia was studied using an microvinification procedure. More precisely, both sugar and enzyme were added to the fruit must before fermentation for half of the samples. This increased the extraction of phenolic compounds. All the samples showed higher bioactivity compared to acarbose, the compound used as a positive control. Blueberry (IC50 ~27 ± 1 µg/ml) and black chokeberry (IC50 ~28 ± 1 µg/ml) wine samples had the highest values regardless of the vinification method. In addition to this, chlorogenic and caffeic acids were recognised as their key AGL bioactives. Taken all together, the fruit wine samples or their lyophilised extracts may be considered as complementary medicine supplements of potential interest for the control of postprandial hyperglycemia.
T2  - Food Bioscience
T1  - Impact of vinification procedure on fruit wine inhibitory activity against α-glucosidase
VL  - 25
SP  - 1
EP  - 7
DO  - 10.1016/j.fbio.2018.06.009
ER  - 
@article{
author = "Čakar, Uroš and Grozdanić, Nađa and Pejin, Boris and Vasić, Vesna M. and Čakar, Mira M. and Petrović, Aleksandar V. and Đorđević, Brižita",
year = "2018",
abstract = "α-Glucosidase inhibitory activity (AGL) of fruit wine samples made from blueberry, black chokeberry, blackberry, raspberry and sour cherry cultivars grown in Serbia was studied using an microvinification procedure. More precisely, both sugar and enzyme were added to the fruit must before fermentation for half of the samples. This increased the extraction of phenolic compounds. All the samples showed higher bioactivity compared to acarbose, the compound used as a positive control. Blueberry (IC50 ~27 ± 1 µg/ml) and black chokeberry (IC50 ~28 ± 1 µg/ml) wine samples had the highest values regardless of the vinification method. In addition to this, chlorogenic and caffeic acids were recognised as their key AGL bioactives. Taken all together, the fruit wine samples or their lyophilised extracts may be considered as complementary medicine supplements of potential interest for the control of postprandial hyperglycemia.",
journal = "Food Bioscience",
title = "Impact of vinification procedure on fruit wine inhibitory activity against α-glucosidase",
volume = "25",
pages = "1-7",
doi = "10.1016/j.fbio.2018.06.009"
}
Čakar, U., Grozdanić, N., Pejin, B., Vasić, V. M., Čakar, M. M., Petrović, A. V.,& Đorđević, B.. (2018). Impact of vinification procedure on fruit wine inhibitory activity against α-glucosidase. in Food Bioscience, 25, 1-7.
https://doi.org/10.1016/j.fbio.2018.06.009
Čakar U, Grozdanić N, Pejin B, Vasić VM, Čakar MM, Petrović AV, Đorđević B. Impact of vinification procedure on fruit wine inhibitory activity against α-glucosidase. in Food Bioscience. 2018;25:1-7.
doi:10.1016/j.fbio.2018.06.009 .
Čakar, Uroš, Grozdanić, Nađa, Pejin, Boris, Vasić, Vesna M., Čakar, Mira M., Petrović, Aleksandar V., Đorđević, Brižita, "Impact of vinification procedure on fruit wine inhibitory activity against α-glucosidase" in Food Bioscience, 25 (2018):1-7,
https://doi.org/10.1016/j.fbio.2018.06.009 . .
24
24
27

Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes

Bondžić, Aleksandra M.; Janjić, Goran V.; Dramićanin, Miroslav; Messori, Luigi; Massai, Lara; Parac Vogt, Tatjana N.; Vasić, Vesna M.

(2017)

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Janjić, Goran V.
AU  - Dramićanin, Miroslav
AU  - Messori, Luigi
AU  - Massai, Lara
AU  - Parac Vogt, Tatjana N.
AU  - Vasić, Vesna M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1476
AB  - Na/K-ATPase is emerging as an important target for a variety of anticancer metal-based drugs. The interactions of Na/K-ATPase (in its E1 state) with three representative and structurally related cytotoxic gold(III) complexes, i.e. [Au(bipy)(OH)(2)][PF6], bipy = 2,2-bipyridine; [Au(py(dmb)-H)(CH3COO)(2)], py(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-pyridine and [Au(bipy(dmb)-H)(OH)][PF6], bipy(c)-H = deprotonated 6-(1,1-dimethylbenzyl)-2,20-bipyridine, are investigated here in depth using a variety of spectroscopic methods, in combination with docking studies. Detailed information is gained on the conformational and structural changes experienced by the enzyme upon binding of these gold(III) complexes. The quenching constants of intrinsic enzyme fluorescence, the fraction of Trp residues accessible to gold(III) complexes and the reaction stoichiometries were determined in various cases. Specific hypotheses are made concerning the binding mode of these gold(III) complexes to the enzyme and the likely binding sites. Differences in their binding behaviour toward Na/K-ATPase are explained on the ground of their distinctive structural features. The present results offer further support to the view that Na/K-ATPase may be a relevant biomolecular target for cytotoxic gold(III) compounds of medicinal interest and may thus be involved in their overall mode of action.
T2  - Metallomics
T1  - Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes
VL  - 9
IS  - 3
SP  - 292
EP  - 300
DO  - 10.1039/c7mt00017k
ER  - 
@article{
author = "Bondžić, Aleksandra M. and Janjić, Goran V. and Dramićanin, Miroslav and Messori, Luigi and Massai, Lara and Parac Vogt, Tatjana N. and Vasić, Vesna M.",
year = "2017",
abstract = "Na/K-ATPase is emerging as an important target for a variety of anticancer metal-based drugs. The interactions of Na/K-ATPase (in its E1 state) with three representative and structurally related cytotoxic gold(III) complexes, i.e. [Au(bipy)(OH)(2)][PF6], bipy = 2,2-bipyridine; [Au(py(dmb)-H)(CH3COO)(2)], py(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-pyridine and [Au(bipy(dmb)-H)(OH)][PF6], bipy(c)-H = deprotonated 6-(1,1-dimethylbenzyl)-2,20-bipyridine, are investigated here in depth using a variety of spectroscopic methods, in combination with docking studies. Detailed information is gained on the conformational and structural changes experienced by the enzyme upon binding of these gold(III) complexes. The quenching constants of intrinsic enzyme fluorescence, the fraction of Trp residues accessible to gold(III) complexes and the reaction stoichiometries were determined in various cases. Specific hypotheses are made concerning the binding mode of these gold(III) complexes to the enzyme and the likely binding sites. Differences in their binding behaviour toward Na/K-ATPase are explained on the ground of their distinctive structural features. The present results offer further support to the view that Na/K-ATPase may be a relevant biomolecular target for cytotoxic gold(III) compounds of medicinal interest and may thus be involved in their overall mode of action.",
journal = "Metallomics",
title = "Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes",
volume = "9",
number = "3",
pages = "292-300",
doi = "10.1039/c7mt00017k"
}
Bondžić, A. M., Janjić, G. V., Dramićanin, M., Messori, L., Massai, L., Parac Vogt, T. N.,& Vasić, V. M.. (2017). Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes. in Metallomics, 9(3), 292-300.
https://doi.org/10.1039/c7mt00017k
Bondžić AM, Janjić GV, Dramićanin M, Messori L, Massai L, Parac Vogt TN, Vasić VM. Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes. in Metallomics. 2017;9(3):292-300.
doi:10.1039/c7mt00017k .
Bondžić, Aleksandra M., Janjić, Goran V., Dramićanin, Miroslav, Messori, Luigi, Massai, Lara, Parac Vogt, Tatjana N., Vasić, Vesna M., "Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes" in Metallomics, 9, no. 3 (2017):292-300,
https://doi.org/10.1039/c7mt00017k . .
12
13
13

The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach

Bondžić, Aleksandra M.; Čolović, Mirjana B.; Janjić, Goran V.; Zarić, Božidarka; Petrović, Sandra; Krstić, Danijela Z.; Marzo, Tiziano; Messori, Luigi; Vasić, Vesna M.

(2017)

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Čolović, Mirjana B.
AU  - Janjić, Goran V.
AU  - Zarić, Božidarka
AU  - Petrović, Sandra
AU  - Krstić, Danijela Z.
AU  - Marzo, Tiziano
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1648
AB  - The in vitro effects of oxo-bridged binuclear gold(III) complexes, i.e., [(bipy2Me)(2)Au-2(mu-O)(2)][PF6](2) (Auoxo6), Au-2[(bipydmb-H)(2)(mu-O)][PF6] (Au(2)bipyC) and [Au-2(phen(2Me))(2)(mu-O)(2)](PF6)(2) (Au(2)phen) on Na/K-ATPase, purified from the porcine cerebral cortex, were investigated. All three studied gold complexes inhibited the enzyme activity in a concentration-dependent manner achieving IC50 values in the low micromolar range. Kinetic analysis suggested an uncompetitive mode of inhibition for Auoxo6 and Au(2)bipyC, and a mixed type one for Au(2)phen. Docking studies indicated that the inhibitory actions of all tested complexes are related to E2-P enzyme conformation binding to ion channel and intracellular part between N and P sub-domain. In addition, Au(2)phen was able to inhibit the enzyme by interacting with its extracellular part as well. Toxic effects of the gold(III) complexes were evaluated in vitro by following lactate dehydrogenase activity in rat brain synaptosomes and incidence of micronuclei and cytokinesis-block proliferation index in cultivated human lymphocytes. All investigated complexes turned out to induce cytogenetic damage consisting of a significant decrease in cell proliferation and an increase in micronuclei in a dose-dependent manner. On the other hand, lactate dehydrogenase activity, an indicator of membrane integrity/viability, was not affected by Auoxo6 and Au(2)bipyC, while Au(2)phen slightly modified its activity.
T2  - Journal of Biological Inorganic Chemistry
T1  - The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach
VL  - 22
IS  - 6
SP  - 819
EP  - 832
DO  - 10.1007/s00775-017-1460-5
ER  - 
@article{
author = "Bondžić, Aleksandra M. and Čolović, Mirjana B. and Janjić, Goran V. and Zarić, Božidarka and Petrović, Sandra and Krstić, Danijela Z. and Marzo, Tiziano and Messori, Luigi and Vasić, Vesna M.",
year = "2017",
abstract = "The in vitro effects of oxo-bridged binuclear gold(III) complexes, i.e., [(bipy2Me)(2)Au-2(mu-O)(2)][PF6](2) (Auoxo6), Au-2[(bipydmb-H)(2)(mu-O)][PF6] (Au(2)bipyC) and [Au-2(phen(2Me))(2)(mu-O)(2)](PF6)(2) (Au(2)phen) on Na/K-ATPase, purified from the porcine cerebral cortex, were investigated. All three studied gold complexes inhibited the enzyme activity in a concentration-dependent manner achieving IC50 values in the low micromolar range. Kinetic analysis suggested an uncompetitive mode of inhibition for Auoxo6 and Au(2)bipyC, and a mixed type one for Au(2)phen. Docking studies indicated that the inhibitory actions of all tested complexes are related to E2-P enzyme conformation binding to ion channel and intracellular part between N and P sub-domain. In addition, Au(2)phen was able to inhibit the enzyme by interacting with its extracellular part as well. Toxic effects of the gold(III) complexes were evaluated in vitro by following lactate dehydrogenase activity in rat brain synaptosomes and incidence of micronuclei and cytokinesis-block proliferation index in cultivated human lymphocytes. All investigated complexes turned out to induce cytogenetic damage consisting of a significant decrease in cell proliferation and an increase in micronuclei in a dose-dependent manner. On the other hand, lactate dehydrogenase activity, an indicator of membrane integrity/viability, was not affected by Auoxo6 and Au(2)bipyC, while Au(2)phen slightly modified its activity.",
journal = "Journal of Biological Inorganic Chemistry",
title = "The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach",
volume = "22",
number = "6",
pages = "819-832",
doi = "10.1007/s00775-017-1460-5"
}
Bondžić, A. M., Čolović, M. B., Janjić, G. V., Zarić, B., Petrović, S., Krstić, D. Z., Marzo, T., Messori, L.,& Vasić, V. M.. (2017). The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach. in Journal of Biological Inorganic Chemistry, 22(6), 819-832.
https://doi.org/10.1007/s00775-017-1460-5
Bondžić AM, Čolović MB, Janjić GV, Zarić B, Petrović S, Krstić DZ, Marzo T, Messori L, Vasić VM. The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach. in Journal of Biological Inorganic Chemistry. 2017;22(6):819-832.
doi:10.1007/s00775-017-1460-5 .
Bondžić, Aleksandra M., Čolović, Mirjana B., Janjić, Goran V., Zarić, Božidarka, Petrović, Sandra, Krstić, Danijela Z., Marzo, Tiziano, Messori, Luigi, Vasić, Vesna M., "The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach" in Journal of Biological Inorganic Chemistry, 22, no. 6 (2017):819-832,
https://doi.org/10.1007/s00775-017-1460-5 . .
6
4
4

Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro

Lazovic, Sasa; Leskovac, Andreja; Petrović, Sandra; Senerović, Lidija; Krivokapić, Nevena; Mitrović, Tatjana; Božović, Nikola; Vasić, Vesna M.; Nikodinović-Runić, Jasmina

(2017)

TY  - JOUR
AU  - Lazovic, Sasa
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Senerović, Lidija
AU  - Krivokapić, Nevena
AU  - Mitrović, Tatjana
AU  - Božović, Nikola
AU  - Vasić, Vesna M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1370
AB  - It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((52)-4-methoxy-5-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5H-2,2-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.
T2  - Experimental and Toxicologic Pathology
T1  - Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro
VL  - 69
IS  - 1
SP  - 55
EP  - 62
DO  - 10.1016/j.etp.2016.11.003
ER  - 
@article{
author = "Lazovic, Sasa and Leskovac, Andreja and Petrović, Sandra and Senerović, Lidija and Krivokapić, Nevena and Mitrović, Tatjana and Božović, Nikola and Vasić, Vesna M. and Nikodinović-Runić, Jasmina",
year = "2017",
abstract = "It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((52)-4-methoxy-5-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5H-2,2-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.",
journal = "Experimental and Toxicologic Pathology",
title = "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro",
volume = "69",
number = "1",
pages = "55-62",
doi = "10.1016/j.etp.2016.11.003"
}
Lazovic, S., Leskovac, A., Petrović, S., Senerović, L., Krivokapić, N., Mitrović, T., Božović, N., Vasić, V. M.,& Nikodinović-Runić, J.. (2017). Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology, 69(1), 55-62.
https://doi.org/10.1016/j.etp.2016.11.003
Lazovic S, Leskovac A, Petrović S, Senerović L, Krivokapić N, Mitrović T, Božović N, Vasić VM, Nikodinović-Runić J. Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology. 2017;69(1):55-62.
doi:10.1016/j.etp.2016.11.003 .
Lazovic, Sasa, Leskovac, Andreja, Petrović, Sandra, Senerović, Lidija, Krivokapić, Nevena, Mitrović, Tatjana, Božović, Nikola, Vasić, Vesna M., Nikodinović-Runić, Jasmina, "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro" in Experimental and Toxicologic Pathology, 69, no. 1 (2017):55-62,
https://doi.org/10.1016/j.etp.2016.11.003 . .
3
3
3

The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro

Petrović, Sandra; Vasić, Vesna M.; Mitrović, Tatjana; Lazovic, Sasa; Leskovac, Andreja

(2017)

TY  - JOUR
AU  - Petrović, Sandra
AU  - Vasić, Vesna M.
AU  - Mitrović, Tatjana
AU  - Lazovic, Sasa
AU  - Leskovac, Andreja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1506
AB  - Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.
T2  - Arhiv za higijenu rada i toksikologiju
T1  - The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro
VL  - 68
IS  - 1
SP  - 1
EP  - 8
DO  - 10.1515/aiht-2017-68-2897
ER  - 
@article{
author = "Petrović, Sandra and Vasić, Vesna M. and Mitrović, Tatjana and Lazovic, Sasa and Leskovac, Andreja",
year = "2017",
abstract = "Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro",
volume = "68",
number = "1",
pages = "1-8",
doi = "10.1515/aiht-2017-68-2897"
}
Petrović, S., Vasić, V. M., Mitrović, T., Lazovic, S.,& Leskovac, A.. (2017). The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro. in Arhiv za higijenu rada i toksikologiju, 68(1), 1-8.
https://doi.org/10.1515/aiht-2017-68-2897
Petrović S, Vasić VM, Mitrović T, Lazovic S, Leskovac A. The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro. in Arhiv za higijenu rada i toksikologiju. 2017;68(1):1-8.
doi:10.1515/aiht-2017-68-2897 .
Petrović, Sandra, Vasić, Vesna M., Mitrović, Tatjana, Lazovic, Sasa, Leskovac, Andreja, "The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro" in Arhiv za higijenu rada i toksikologiju, 68, no. 1 (2017):1-8,
https://doi.org/10.1515/aiht-2017-68-2897 . .
7
5
5

Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs

Lazarević-Pašti, Tamara; Leskovac, Andreja; Momić, Tatjana; Petrović, Sandra; Vasić, Vesna M.

(2017)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Leskovac, Andreja
AU  - Momić, Tatjana
AU  - Petrović, Sandra
AU  - Vasić, Vesna M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1796
AB  - Background: Acetylcholinesterase (AChE) is involved in the termination of impulse transmission by rapid hydrolysis of the neurotransmitter acetylcholine in numerous cholinergic pathways in the central and peripheral nervous systems. The enzyme inactivation leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. Hence, acetylcholinesterase inhibitors, interacting with the enzyme as their primary target, are applied as relevant drugs for different neurodegenerative diseases (such as Alzheimers and Parkinsons) as well as toxins. At the same time, there are increasing evidence that in non-neuronal context, AChE is involved in the regulation of cell proliferation, differentiation, apoptosis and cell-cell interaction. An irregular expression of AChE has been found in different types of tumors, suggesting the involvement of AChE in the regulation of tumor development. Having all this in mind, there is a possibility that some AChE inhibitors could be used as anti-cancer agents. Objective: This contribution will discuss a broad range of possible application of different AChE inhibitors as drugs, from well-known anti-Alzheimers disease drugs to their use in cancer treatment in future. Emphasis will be put on various known AChE inhibitors classes, whose application as drugs could be controversy, as well as on newly investigated natural products, which can also modulate AChE activity. Conclusion: It is not clear a patient treated for neurodegenerative condition prone to increased risk for some types of cancer and vice versa. This is necessary to keep in mind during rational drug design process for all therapies, which are based on AChE as a target molecule.
T2  - Current Medicinal Chemistry
T1  - Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs
VL  - 24
IS  - 30
SP  - 3283
EP  - 3309
DO  - 10.2174/0929867324666170705123509
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Leskovac, Andreja and Momić, Tatjana and Petrović, Sandra and Vasić, Vesna M.",
year = "2017",
abstract = "Background: Acetylcholinesterase (AChE) is involved in the termination of impulse transmission by rapid hydrolysis of the neurotransmitter acetylcholine in numerous cholinergic pathways in the central and peripheral nervous systems. The enzyme inactivation leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. Hence, acetylcholinesterase inhibitors, interacting with the enzyme as their primary target, are applied as relevant drugs for different neurodegenerative diseases (such as Alzheimers and Parkinsons) as well as toxins. At the same time, there are increasing evidence that in non-neuronal context, AChE is involved in the regulation of cell proliferation, differentiation, apoptosis and cell-cell interaction. An irregular expression of AChE has been found in different types of tumors, suggesting the involvement of AChE in the regulation of tumor development. Having all this in mind, there is a possibility that some AChE inhibitors could be used as anti-cancer agents. Objective: This contribution will discuss a broad range of possible application of different AChE inhibitors as drugs, from well-known anti-Alzheimers disease drugs to their use in cancer treatment in future. Emphasis will be put on various known AChE inhibitors classes, whose application as drugs could be controversy, as well as on newly investigated natural products, which can also modulate AChE activity. Conclusion: It is not clear a patient treated for neurodegenerative condition prone to increased risk for some types of cancer and vice versa. This is necessary to keep in mind during rational drug design process for all therapies, which are based on AChE as a target molecule.",
journal = "Current Medicinal Chemistry",
title = "Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs",
volume = "24",
number = "30",
pages = "3283-3309",
doi = "10.2174/0929867324666170705123509"
}
Lazarević-Pašti, T., Leskovac, A., Momić, T., Petrović, S.,& Vasić, V. M.. (2017). Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs. in Current Medicinal Chemistry, 24(30), 3283-3309.
https://doi.org/10.2174/0929867324666170705123509
Lazarević-Pašti T, Leskovac A, Momić T, Petrović S, Vasić VM. Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs. in Current Medicinal Chemistry. 2017;24(30):3283-3309.
doi:10.2174/0929867324666170705123509 .
Lazarević-Pašti, Tamara, Leskovac, Andreja, Momić, Tatjana, Petrović, Sandra, Vasić, Vesna M., "Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs" in Current Medicinal Chemistry, 24, no. 30 (2017):3283-3309,
https://doi.org/10.2174/0929867324666170705123509 . .
7
55
46
45

Modulation of acetylcholinesterase activity induced by polyoxotungstates

Čolović, Mirjana B.; Bondžić, Aleksandra M.; Kortz, U.; Vasić, Vesna M.; Krstić, Danijela Z.

(Society of Physical Chemists of Serbia, 2016)

TY  - CONF
AU  - Čolović, Mirjana B.
AU  - Bondžić, Aleksandra M.
AU  - Kortz, U.
AU  - Vasić, Vesna M.
AU  - Krstić, Danijela Z.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9208
AB  - The in  vitroinfluence  of  five  polyoxotungstates  containing  various  central atoms    on    acetylcholinesterase    (AChE)    activity    was    investigated. K6[PV3W9O40] × 3H2O, K6H2[TiW11CoO40] × 13H2O, (NH4)14[NaP5W30O110]    ×    31H2O,    K7[SiV3W9O40]    ×    10H2O,    and K7[Ti2PW10O40]   induced   the   enzyme   inhibition   in   a   concentration-dependent  manner.  Inhibitory  power  of  the  investigated  compounds  was evaluated  using  IC50values.  K7[SiV3W9O40]  ×  10H2O  affected  AChE activity with lowest potency (IC50 = 4.80 × 10-4mol/L). K6H2[TiW11CoO40] ×  13H2O  and  K7[Ti2PW10O40]  exhibited  high  affinity  toward  the  enzyme, inducing half-maximuminhibition at micromolar concentrations (1.14 × 10-6and 1.04 × 10-6mol/L, respectively), while the same effect was achieved in the  presence  of  about  fifty  times  higher  concentration  of  K6[PV3W9O40]  × 3H2O.  Finally,  (NH4)14[NaP5W30O110]  ×  31H2O  was  foundas  the  most potent   inhibitor   of   AChE   activity   (IC50 = 6.36   ×   10-7mol/L),   and consequently the most promising candidate for the treatment of neurological diseases associated with acetylcholine leakage.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
T1  - Modulation of acetylcholinesterase activity induced by polyoxotungstates
SP  - 451
EP  - 454
ER  - 
@conference{
author = "Čolović, Mirjana B. and Bondžić, Aleksandra M. and Kortz, U. and Vasić, Vesna M. and Krstić, Danijela Z.",
year = "2016",
abstract = "The in  vitroinfluence  of  five  polyoxotungstates  containing  various  central atoms    on    acetylcholinesterase    (AChE)    activity    was    investigated. K6[PV3W9O40] × 3H2O, K6H2[TiW11CoO40] × 13H2O, (NH4)14[NaP5W30O110]    ×    31H2O,    K7[SiV3W9O40]    ×    10H2O,    and K7[Ti2PW10O40]   induced   the   enzyme   inhibition   in   a   concentration-dependent  manner.  Inhibitory  power  of  the  investigated  compounds  was evaluated  using  IC50values.  K7[SiV3W9O40]  ×  10H2O  affected  AChE activity with lowest potency (IC50 = 4.80 × 10-4mol/L). K6H2[TiW11CoO40] ×  13H2O  and  K7[Ti2PW10O40]  exhibited  high  affinity  toward  the  enzyme, inducing half-maximuminhibition at micromolar concentrations (1.14 × 10-6and 1.04 × 10-6mol/L, respectively), while the same effect was achieved in the  presence  of  about  fifty  times  higher  concentration  of  K6[PV3W9O40]  × 3H2O.  Finally,  (NH4)14[NaP5W30O110]  ×  31H2O  was  foundas  the  most potent   inhibitor   of   AChE   activity   (IC50 = 6.36   ×   10-7mol/L),   and consequently the most promising candidate for the treatment of neurological diseases associated with acetylcholine leakage.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry",
title = "Modulation of acetylcholinesterase activity induced by polyoxotungstates",
pages = "451-454"
}
Čolović, M. B., Bondžić, A. M., Kortz, U., Vasić, V. M.,& Krstić, D. Z.. (2016). Modulation of acetylcholinesterase activity induced by polyoxotungstates. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 451-454.
Čolović MB, Bondžić AM, Kortz U, Vasić VM, Krstić DZ. Modulation of acetylcholinesterase activity induced by polyoxotungstates. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry. 2016;:451-454..
Čolović, Mirjana B., Bondžić, Aleksandra M., Kortz, U., Vasić, Vesna M., Krstić, Danijela Z., "Modulation of acetylcholinesterase activity induced by polyoxotungstates" in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry (2016):451-454.

Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water

Lazarević-Pašti, Tamara; Pašti, Igor A.; Jokić, Bojan M.; Babić, Biljana M.; Vasić, Vesna M.

(2016)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Pašti, Igor A.
AU  - Jokić, Bojan M.
AU  - Babić, Biljana M.
AU  - Vasić, Vesna M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1178
AB  - Extensive use of organophosphate pesticides (OPs) invokes development of efficient procedures for their removal from the environment. By introducing low levels ( LT 1 at%) of B, N or P into the structure of mesoporous carbons, we have produced a series of materials with different surface chemical composition, textural properties and level of structural disorder. These adsorbents were applied for removal of dimethoate and its oxo-analogue omethoate from aqueous solutions under batch adsorption conditions and by filtration using modified nylon membrane filters. Adsorption capacities up to 164 mg g(-1) were measured, with OPs uptake typically above 80% for dimethoate concentration as high as 5 x 10(-3) mol dm(-3). After the adsorption, neurotoxic effects of OP-containing water samples were significantly reduced or completely removed. The level of structural disorder was identified as a key parameter for efficient removal of dimethoate and omethoate while in the filtration experiments surface area of adsorbents also played an important role. While the presented research appeals to new fundamental studies of OP-carbon surface interactions, it also indicates a possible strategy in designing new efficient adsorbents for OPs removal from water.
T2  - RSC Advances
T1  - Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water
VL  - 6
IS  - 67
SP  - 62128
EP  - 62139
DO  - 10.1039/c6ra06736k
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Pašti, Igor A. and Jokić, Bojan M. and Babić, Biljana M. and Vasić, Vesna M.",
year = "2016",
abstract = "Extensive use of organophosphate pesticides (OPs) invokes development of efficient procedures for their removal from the environment. By introducing low levels ( LT 1 at%) of B, N or P into the structure of mesoporous carbons, we have produced a series of materials with different surface chemical composition, textural properties and level of structural disorder. These adsorbents were applied for removal of dimethoate and its oxo-analogue omethoate from aqueous solutions under batch adsorption conditions and by filtration using modified nylon membrane filters. Adsorption capacities up to 164 mg g(-1) were measured, with OPs uptake typically above 80% for dimethoate concentration as high as 5 x 10(-3) mol dm(-3). After the adsorption, neurotoxic effects of OP-containing water samples were significantly reduced or completely removed. The level of structural disorder was identified as a key parameter for efficient removal of dimethoate and omethoate while in the filtration experiments surface area of adsorbents also played an important role. While the presented research appeals to new fundamental studies of OP-carbon surface interactions, it also indicates a possible strategy in designing new efficient adsorbents for OPs removal from water.",
journal = "RSC Advances",
title = "Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water",
volume = "6",
number = "67",
pages = "62128-62139",
doi = "10.1039/c6ra06736k"
}
Lazarević-Pašti, T., Pašti, I. A., Jokić, B. M., Babić, B. M.,& Vasić, V. M.. (2016). Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water. in RSC Advances, 6(67), 62128-62139.
https://doi.org/10.1039/c6ra06736k
Lazarević-Pašti T, Pašti IA, Jokić BM, Babić BM, Vasić VM. Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water. in RSC Advances. 2016;6(67):62128-62139.
doi:10.1039/c6ra06736k .
Lazarević-Pašti, Tamara, Pašti, Igor A., Jokić, Bojan M., Babić, Biljana M., Vasić, Vesna M., "Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water" in RSC Advances, 6, no. 67 (2016):62128-62139,
https://doi.org/10.1039/c6ra06736k . .
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18
19

Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods

Momić, Tatjana; Lazarević-Pašti, Tamara; Bogdanović, Una; Vodnik, Vesna; Mraković, Ana Đ.; Rakočević, Zlatko Lj.; Pavlović, Vladimir B.; Vasić, Vesna M.

(2016)

TY  - JOUR
AU  - Momić, Tatjana
AU  - Lazarević-Pašti, Tamara
AU  - Bogdanović, Una
AU  - Vodnik, Vesna
AU  - Mraković, Ana Đ.
AU  - Rakočević, Zlatko Lj.
AU  - Pavlović, Vladimir B.
AU  - Vasić, Vesna M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1347
AB  - Organophosphorus pesticide dimethoate was adsorbed onto gold nanospheres and nanorods in aqueous solution using batch technique. Adsorption of dimethoate onto gold nanoparticles was confirmed by UV-Vis spectrophotometry, TEM, AFM, and FTIR analysis. The adsorption of nanospheres resulted in aggregation which was not the case with nanorods. Nanoparticles adsorption features were characterized using Langmuir and Freundlich isotherm models. The Langmuir adsorption isotherm was found to have the best fit to the experimental data for both types of nanoparticles. Adsorption capacity detected for nanospheres is 456 mg/g and for nanorods is 57.1 mg/g. Also, nanoparticles were successfully used for dimethoate removal from spiked drinking water while nanospheres were shown to be more efficient than nanorods.
T2  - Journal of Nanomaterials
T1  - Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods
DO  - 10.1155/2016/8910271
ER  - 
@article{
author = "Momić, Tatjana and Lazarević-Pašti, Tamara and Bogdanović, Una and Vodnik, Vesna and Mraković, Ana Đ. and Rakočević, Zlatko Lj. and Pavlović, Vladimir B. and Vasić, Vesna M.",
year = "2016",
abstract = "Organophosphorus pesticide dimethoate was adsorbed onto gold nanospheres and nanorods in aqueous solution using batch technique. Adsorption of dimethoate onto gold nanoparticles was confirmed by UV-Vis spectrophotometry, TEM, AFM, and FTIR analysis. The adsorption of nanospheres resulted in aggregation which was not the case with nanorods. Nanoparticles adsorption features were characterized using Langmuir and Freundlich isotherm models. The Langmuir adsorption isotherm was found to have the best fit to the experimental data for both types of nanoparticles. Adsorption capacity detected for nanospheres is 456 mg/g and for nanorods is 57.1 mg/g. Also, nanoparticles were successfully used for dimethoate removal from spiked drinking water while nanospheres were shown to be more efficient than nanorods.",
journal = "Journal of Nanomaterials",
title = "Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods",
doi = "10.1155/2016/8910271"
}
Momić, T., Lazarević-Pašti, T., Bogdanović, U., Vodnik, V., Mraković, A. Đ., Rakočević, Z. Lj., Pavlović, V. B.,& Vasić, V. M.. (2016). Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods. in Journal of Nanomaterials.
https://doi.org/10.1155/2016/8910271
Momić T, Lazarević-Pašti T, Bogdanović U, Vodnik V, Mraković AĐ, Rakočević ZL, Pavlović VB, Vasić VM. Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods. in Journal of Nanomaterials. 2016;.
doi:10.1155/2016/8910271 .
Momić, Tatjana, Lazarević-Pašti, Tamara, Bogdanović, Una, Vodnik, Vesna, Mraković, Ana Đ., Rakočević, Zlatko Lj., Pavlović, Vladimir B., Vasić, Vesna M., "Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods" in Journal of Nanomaterials (2016),
https://doi.org/10.1155/2016/8910271 . .
36
23
24

Mechanism of 3,3 -Disulfopropyl-5,5 -Dichlorothiacyanine Anion Interaction With Citrate-Capped Silver Nanoparticles: Adsorption and J-Aggregation

Laban, Bojana B.; Zeković, Ivana Lj.; Vasić Anićijević, Dragana D.; Marković, Mirjana; Vodnik, Vesna; Luce, Marco; Cricenti, Antonio; Dramićanin, Miroslav; Vasić, Vesna M.

(2016)

TY  - JOUR
AU  - Laban, Bojana B.
AU  - Zeković, Ivana Lj.
AU  - Vasić Anićijević, Dragana D.
AU  - Marković, Mirjana
AU  - Vodnik, Vesna
AU  - Luce, Marco
AU  - Cricenti, Antonio
AU  - Dramićanin, Miroslav
AU  - Vasić, Vesna M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1212
AB  - The paper presents the results of the experimental and theoretical study of 3,3-disulfopropyl-5,5-dichlorothiacyanine sodium salt (TC) adsorption and J-aggregation on the surface of citrate-capped silver nano particles (AgNPs) with an average particle size 10 nm. Various nanospectroscopy techniques such as UV-vis, TEM, AFM, DLS, zeta potential, and fluorescence measurements were carried out in order to characterize the hybrid system. Besides this, a set of simple density functional theory calculations (DFT) was performed and this suggested that TC dye, from the thermodynamical point of view, most likely interacted with AgNPs via oxygen atom from SO3- groups. The methods, which considered AgNPs as the macromolecule with several binding sites and TC dye as the ligand, were applied for the analysis of saturation curves constructed from the fluorescence data to obtain the binding constant (K-a = 1.5 x 10(6) M-1) and the average number of TC dye molecules bonded per AgNP (similar to 330). Moreover, TC fluorescence was quenched in the presence of AgNPs on the concentration dependent manner, yielding the linear Stern-Volmer relation accounting for both static and dynamic quenching with the quenching constant K-sv = 2.5 x 10(8) M-1. Kinetic measurements of J-aggregation as the dependence of AgNPs/TC concentration ratio confirmed that the J-aggregation occurred via a two-step process, the first of them being the initial adsorption of dye on AgNPs surface, followed by dye molecules rearrangement and the consecutive growth of TC aggregate layers.
T2  - Journal of Physical Chemistry. C
T1  - Mechanism of 3,3 -Disulfopropyl-5,5 -Dichlorothiacyanine Anion Interaction With Citrate-Capped Silver Nanoparticles: Adsorption and J-Aggregation
VL  - 120
IS  - 32
SP  - 18066
EP  - 18074
DO  - 10.1021/acs.jpcc.6b05124
ER  - 
@article{
author = "Laban, Bojana B. and Zeković, Ivana Lj. and Vasić Anićijević, Dragana D. and Marković, Mirjana and Vodnik, Vesna and Luce, Marco and Cricenti, Antonio and Dramićanin, Miroslav and Vasić, Vesna M.",
year = "2016",
abstract = "The paper presents the results of the experimental and theoretical study of 3,3-disulfopropyl-5,5-dichlorothiacyanine sodium salt (TC) adsorption and J-aggregation on the surface of citrate-capped silver nano particles (AgNPs) with an average particle size 10 nm. Various nanospectroscopy techniques such as UV-vis, TEM, AFM, DLS, zeta potential, and fluorescence measurements were carried out in order to characterize the hybrid system. Besides this, a set of simple density functional theory calculations (DFT) was performed and this suggested that TC dye, from the thermodynamical point of view, most likely interacted with AgNPs via oxygen atom from SO3- groups. The methods, which considered AgNPs as the macromolecule with several binding sites and TC dye as the ligand, were applied for the analysis of saturation curves constructed from the fluorescence data to obtain the binding constant (K-a = 1.5 x 10(6) M-1) and the average number of TC dye molecules bonded per AgNP (similar to 330). Moreover, TC fluorescence was quenched in the presence of AgNPs on the concentration dependent manner, yielding the linear Stern-Volmer relation accounting for both static and dynamic quenching with the quenching constant K-sv = 2.5 x 10(8) M-1. Kinetic measurements of J-aggregation as the dependence of AgNPs/TC concentration ratio confirmed that the J-aggregation occurred via a two-step process, the first of them being the initial adsorption of dye on AgNPs surface, followed by dye molecules rearrangement and the consecutive growth of TC aggregate layers.",
journal = "Journal of Physical Chemistry. C",
title = "Mechanism of 3,3 -Disulfopropyl-5,5 -Dichlorothiacyanine Anion Interaction With Citrate-Capped Silver Nanoparticles: Adsorption and J-Aggregation",
volume = "120",
number = "32",
pages = "18066-18074",
doi = "10.1021/acs.jpcc.6b05124"
}
Laban, B. B., Zeković, I. Lj., Vasić Anićijević, D. D., Marković, M., Vodnik, V., Luce, M., Cricenti, A., Dramićanin, M.,& Vasić, V. M.. (2016). Mechanism of 3,3 -Disulfopropyl-5,5 -Dichlorothiacyanine Anion Interaction With Citrate-Capped Silver Nanoparticles: Adsorption and J-Aggregation. in Journal of Physical Chemistry. C, 120(32), 18066-18074.
https://doi.org/10.1021/acs.jpcc.6b05124
Laban BB, Zeković IL, Vasić Anićijević DD, Marković M, Vodnik V, Luce M, Cricenti A, Dramićanin M, Vasić VM. Mechanism of 3,3 -Disulfopropyl-5,5 -Dichlorothiacyanine Anion Interaction With Citrate-Capped Silver Nanoparticles: Adsorption and J-Aggregation. in Journal of Physical Chemistry. C. 2016;120(32):18066-18074.
doi:10.1021/acs.jpcc.6b05124 .
Laban, Bojana B., Zeković, Ivana Lj., Vasić Anićijević, Dragana D., Marković, Mirjana, Vodnik, Vesna, Luce, Marco, Cricenti, Antonio, Dramićanin, Miroslav, Vasić, Vesna M., "Mechanism of 3,3 -Disulfopropyl-5,5 -Dichlorothiacyanine Anion Interaction With Citrate-Capped Silver Nanoparticles: Adsorption and J-Aggregation" in Journal of Physical Chemistry. C, 120, no. 32 (2016):18066-18074,
https://doi.org/10.1021/acs.jpcc.6b05124 . .
13
10
12

Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase

Nastasijević, Branislav J.; Milošević, Maja; Janjić, Goran V.; Stanić, Vojislav; Vasić, Vesna M.

(2016)

TY  - JOUR
AU  - Nastasijević, Branislav J.
AU  - Milošević, Maja
AU  - Janjić, Goran V.
AU  - Stanić, Vojislav
AU  - Vasić, Vesna M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1197
AB  - The extracellular nucleotides act as neurotransmitters and signaling molecules in CNS, binding to a P2X and P2Y receptors. Their concentration regulates a cascade of membrane ecto enzymes, including the ecto-nucleotide triphosphate diphosphohydrolases (E-NTPDases). In many neuropathological conditions, such as neuroinflammatory, epilepsy, depression and migraine, altering of E-NTPDase activity was observed. The objective of this study was to investigate whether Gentiana lutea (G. lutea) extracts affect E-NTPDase activity and which of their constituents (loganic acid, gentiopicroside, isovitexin, amarogentin and isogentisin) exert inhibitory activity. The constituents concentration in the extracts was determined by ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS). Extracts and constituents were tested with E-NTPDase displayed on the rat synaptosomal membrane as well as by molecular docking study. Ethanol water extract (50%, v/v) exerted significant level of inhibition (52%) at concentration of 200 mg mL(-1). By inhibition studies with single constituents about 30% inhibition was achieved in any case, thus the model of one substrate acting on two enzymes was used to determine IC50 values. Molecular docking study revealed amarogentin, isovitexin and isogentisin dimer as the potent E-NTPDase inhibitors with the binding energies ranging from -9.4 to -10 kcal mol(-1) versus -8.0 kcal mol(-1) for ATP. Presence of isogentisin only in ethanol water extracts may explain their better inhibitory acitivities. Findings of this study are useful from the perspective of safety of products based on G. lutea extracts, while investigated constituents belong to secoiridoids and xanthones class of compounds could be considered as a source of potential E-NTPDase inhibitors.
T2  - International Journal of Pharmacology
T1  - Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase
VL  - 12
IS  - 4
SP  - 272
EP  - 289
DO  - 10.3923/ijp.2016.272.289
ER  - 
@article{
author = "Nastasijević, Branislav J. and Milošević, Maja and Janjić, Goran V. and Stanić, Vojislav and Vasić, Vesna M.",
year = "2016",
abstract = "The extracellular nucleotides act as neurotransmitters and signaling molecules in CNS, binding to a P2X and P2Y receptors. Their concentration regulates a cascade of membrane ecto enzymes, including the ecto-nucleotide triphosphate diphosphohydrolases (E-NTPDases). In many neuropathological conditions, such as neuroinflammatory, epilepsy, depression and migraine, altering of E-NTPDase activity was observed. The objective of this study was to investigate whether Gentiana lutea (G. lutea) extracts affect E-NTPDase activity and which of their constituents (loganic acid, gentiopicroside, isovitexin, amarogentin and isogentisin) exert inhibitory activity. The constituents concentration in the extracts was determined by ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS). Extracts and constituents were tested with E-NTPDase displayed on the rat synaptosomal membrane as well as by molecular docking study. Ethanol water extract (50%, v/v) exerted significant level of inhibition (52%) at concentration of 200 mg mL(-1). By inhibition studies with single constituents about 30% inhibition was achieved in any case, thus the model of one substrate acting on two enzymes was used to determine IC50 values. Molecular docking study revealed amarogentin, isovitexin and isogentisin dimer as the potent E-NTPDase inhibitors with the binding energies ranging from -9.4 to -10 kcal mol(-1) versus -8.0 kcal mol(-1) for ATP. Presence of isogentisin only in ethanol water extracts may explain their better inhibitory acitivities. Findings of this study are useful from the perspective of safety of products based on G. lutea extracts, while investigated constituents belong to secoiridoids and xanthones class of compounds could be considered as a source of potential E-NTPDase inhibitors.",
journal = "International Journal of Pharmacology",
title = "Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase",
volume = "12",
number = "4",
pages = "272-289",
doi = "10.3923/ijp.2016.272.289"
}
Nastasijević, B. J., Milošević, M., Janjić, G. V., Stanić, V.,& Vasić, V. M.. (2016). Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase. in International Journal of Pharmacology, 12(4), 272-289.
https://doi.org/10.3923/ijp.2016.272.289
Nastasijević BJ, Milošević M, Janjić GV, Stanić V, Vasić VM. Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase. in International Journal of Pharmacology. 2016;12(4):272-289.
doi:10.3923/ijp.2016.272.289 .
Nastasijević, Branislav J., Milošević, Maja, Janjić, Goran V., Stanić, Vojislav, Vasić, Vesna M., "Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase" in International Journal of Pharmacology, 12, no. 4 (2016):272-289,
https://doi.org/10.3923/ijp.2016.272.289 . .
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