TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Iannolo, Gioacchin
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6905
AB  - Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-mu M of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 mu M of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.
T2  - Radiation Protection Dosimetry
T1  - Proton inactivation of melanoma cells enhanced by fotemustine
VL  - 143
IS  - 2-4
SP  - 503
EP  - 507
DO  - 10.1093/rpd/ncq527
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Iannolo, Gioacchin and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2011",
abstract = "Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-mu M of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 mu M of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.",
journal = "Radiation Protection Dosimetry",
title = "Proton inactivation of melanoma cells enhanced by fotemustine",
volume = "143",
number = "2-4",
pages = "503-507",
doi = "10.1093/rpd/ncq527"
}

Ristić-Fira, A., Korićanac, L., Žakula, J., Keta, O. D., Iannolo, G., Cuttone, G.,& Petrović, I. M.. (2011). Proton inactivation of melanoma cells enhanced by fotemustine. in Radiation Protection Dosimetry, 143(2-4), 503-507.
https://doi.org/10.1093/rpd/ncq527

Ristić-Fira A, Korićanac L, Žakula J, Keta OD, Iannolo G, Cuttone G, Petrović IM. Proton inactivation of melanoma cells enhanced by fotemustine. in Radiation Protection Dosimetry. 2011;143(2-4):503-507.
doi:10.1093/rpd/ncq527 .

Ristić-Fira, Aleksandra, Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Iannolo, Gioacchin, Cuttone, Giacomo, Petrović, Ivan M., "Proton inactivation of melanoma cells enhanced by fotemustine" in Radiation Protection Dosimetry, 143, no. 2-4 (2011):503-507,
https://doi.org/10.1093/rpd/ncq527 . .

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Valastro, Lucia M.
AU  - Iannolo, Gioacchin
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3702
AB  - Background: Considering that HTB140 melanoma cells have shown a poor response to either protons or alkylating agents, the effects of a combined use of these agents have been analysed. Methods: Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak (SOBP). Irradiation doses were 12 or 16 Gy and are those frequently used in proton therapy. Four days after irradiation cells were treated with fotemustine (FM) or dacarbazine (DTIC). Drug concentrations were 100 and 250 mu M, values close to those that produce 50% of growth inhibition. Cell viability, proliferation, survival and cell cycle distribution were assessed 7 days after irradiation that corresponds to more than six doubling times of HTB140 cells. In this way incubation periods providing the best single effects of drugs (3 days) and protons (7 days) coincided at the same time. Results: Single proton irradiations have reduced the number of cells to similar to 50%. FM caused stronger cell inactivation due to its high toxicity, while the effectiveness of DTIC, that was important at short term, almost vanished with the incubation of 7 days. Cellular mechanisms triggered by proton irradiation differently influenced the final effects of combined treatments. Combination of protons and FM did not improve cell inactivation level achieved by single treatments. A low efficiency of the single DTIC treatment was overcome when DTIC was introduced following proton irradiation, giving better inhibitory effects with respect to the single treatments. Most of the analysed cells were in G1/S phase, viable, active and able to replicate DNA. Conclusion: The obtained results are the consequence of a high resistance of HTB140 melanoma cells to protons and/or drugs. The inactivation level of the HTB140 human melanoma cells after protons, FM or DTIC treatments was not enhanced by their combined application.
T2  - Journal of Experimental and Clinical Cancer Research
T1  - Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation
VL  - 28
DO  - 10.1186/1756-9966-28-50
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Korićanac, Lela and Žakula, Jelena and Valastro, Lucia M. and Iannolo, Gioacchin and Privitera, Giuseppe and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2009",
abstract = "Background: Considering that HTB140 melanoma cells have shown a poor response to either protons or alkylating agents, the effects of a combined use of these agents have been analysed. Methods: Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak (SOBP). Irradiation doses were 12 or 16 Gy and are those frequently used in proton therapy. Four days after irradiation cells were treated with fotemustine (FM) or dacarbazine (DTIC). Drug concentrations were 100 and 250 mu M, values close to those that produce 50% of growth inhibition. Cell viability, proliferation, survival and cell cycle distribution were assessed 7 days after irradiation that corresponds to more than six doubling times of HTB140 cells. In this way incubation periods providing the best single effects of drugs (3 days) and protons (7 days) coincided at the same time. Results: Single proton irradiations have reduced the number of cells to similar to 50%. FM caused stronger cell inactivation due to its high toxicity, while the effectiveness of DTIC, that was important at short term, almost vanished with the incubation of 7 days. Cellular mechanisms triggered by proton irradiation differently influenced the final effects of combined treatments. Combination of protons and FM did not improve cell inactivation level achieved by single treatments. A low efficiency of the single DTIC treatment was overcome when DTIC was introduced following proton irradiation, giving better inhibitory effects with respect to the single treatments. Most of the analysed cells were in G1/S phase, viable, active and able to replicate DNA. Conclusion: The obtained results are the consequence of a high resistance of HTB140 melanoma cells to protons and/or drugs. The inactivation level of the HTB140 human melanoma cells after protons, FM or DTIC treatments was not enhanced by their combined application.",
journal = "Journal of Experimental and Clinical Cancer Research",
title = "Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation",
volume = "28",
doi = "10.1186/1756-9966-28-50"
}

Ristić-Fira, A., Korićanac, L., Žakula, J., Valastro, L. M., Iannolo, G., Privitera, G., Cuttone, G.,& Petrović, I. M.. (2009). Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation. in Journal of Experimental and Clinical Cancer Research, 28.
https://doi.org/10.1186/1756-9966-28-50

Ristić-Fira A, Korićanac L, Žakula J, Valastro LM, Iannolo G, Privitera G, Cuttone G, Petrović IM. Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation. in Journal of Experimental and Clinical Cancer Research. 2009;28.
doi:10.1186/1756-9966-28-50 .

Ristić-Fira, Aleksandra, Korićanac, Lela, Žakula, Jelena, Valastro, Lucia M., Iannolo, Gioacchin, Privitera, Giuseppe, Cuttone, Giacomo, Petrović, Ivan M., "Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation" in Journal of Experimental and Clinical Cancer Research, 28 (2009),
https://doi.org/10.1186/1756-9966-28-50 . .