TY  - JOUR
AU  - Knežević, Dragan
AU  - Bošnjaković, Dušan
AU  - Dražić, Slavica
AU  - Nedić, Sreten
AU  - Vujanac, Ivan
AU  - Valčić, Olivera
AU  - Pantelić, Marija
AU  - Stojiljković, Mojca
AU  - Sladojević, Željko
AU  - Kirovski, Danijela
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13027
AB  - The study aimed to determine the concentration of energy-related hormones in cow’s milk and to consider them from a public health perspective. Fourteen Holstein cows were subjected to milk and blood sampling on the following days in lactation (DIL): 10, 30, 60, 90, 150, 180, 210, 250 and 280 to determine milk hormones, fat and protein content and blood biochemical parameters. For the same purpose, bulk-tank milk was sampled and samples of retail m ilk with 1.5% (CM1.5) and 3.2% (CM3.2) fat was purchased. Milk insulin-like growth factor-1 (IGF-1) values were signifi cantly lower at 90, 150, 180, 210 and 250 and signifi cantly higher at 10, 30 and 60 DIL than lactation average (LA). Milk insulin concentrations were signifi cantly lower at 30, 60 and 90 and higher at 210, 250 and 280 DIL than LA. Free thyroxine (fT4) level in the milk was higher at 250 DIL, while milk free triiodothyronine (fT3) concentrations were lower at 30, 60, 90 and 280 DIL, and signifi cantly higher at 10 and 180 DIL than respective LA. Milk cortisol levels were lower at 60 and 280 DIL than LA. All measured milk hormones were signifi cantly lower in CM1.5 compared to CM3.2, bulk-tank milk and LA. An exception was the LA of IGF-1, which was signifi cantly lower than the IGF1 content in CM1.5. Blood biochemical parameters fl uctuated evenly during lactation and were within the reference range. Hormone concentrations in cow’s milk fl uctuate during lactation, giving milk an important role in the context of public health.
AB  - Istraživanje je imalo za cilj određivanje koncentracije hormona u mleku krava u različitim fazama laktacije, mleku iz laktofriza i maloprodajnom mleku. Uzorci mleka i krvi prikupljeni su 10., 30., 60., 90., 150., 180., 210., 250. i 280 dana laktacije od četrnaest krava holštajn rase radi određivanja koncentracije hormona, masti i proteina u mleku i biohemijskih parametara krvi. U iste svrhe uzorkovano je mleko iz laktofriza i nabavljeno je komercijalno mleko sa 1,5% (CM1,5) i 3,2% (CM3,2) mlečne masti. Koncentracije insulinu-sličnog faktora rasta-1 (IGF-1) u mleku bile su značajno niže 90., 150., 180., 210. i 250. dana i značajno više 10., 30. i 60. dana laktacije poredeći sa laktacionim prosekom (LP). Insulin je imao značajno niže koncentracije u mleku 30., 60. i 90. dana i značajno više koncentracije 210., 250. i 280. dana laktacije poredeći sa LP. Koncentracija slobodnog tiroksina (fT4) je bila viša 250. dana, dok je nivo slobodnog trijodotironina (fT3) bio značajno niži 30., 60., 90. i 280. dana i značajno viši 10. i 180. dana u poređenju sa odgovarajućim LP. Nivo kortizola u mleku je bio niži 60. i 280. dana laktacije poredeći sa LP. Svi hormoni su imali značajno niže koncentracije u CM1,5 nego u CM3,2, mleku iz laktofriza i LP. Izuzetak je LP za IGF-1, koji je bio značajno niži nego nivo IGF-1 u CM1,5. Biohemijski parametri krvi su blago fl uktuirali tokom laktacije, ali su ostali u referentnom opsegu. Koncentracije hormona u kravljem mleku fl uktuiraju tokom laktacije, što daje značaj mleku u kontekstu javnog zdravlja.
T2  - Acta Veterinaria
T1  - Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers
T1  - Hormoni u sirovom i maloprodajnom mleku krava i potencijalni rizik po zdravlje potrošača
VL  - 74
IS  - 1
SP  - 1
EP  - 16
DO  - 10.2478/acve-2024-0001
ER  - 

@article{
author = "Knežević, Dragan and Bošnjaković, Dušan and Dražić, Slavica and Nedić, Sreten and Vujanac, Ivan and Valčić, Olivera and Pantelić, Marija and Stojiljković, Mojca and Sladojević, Željko and Kirovski, Danijela",
year = "2024",
abstract = "The study aimed to determine the concentration of energy-related hormones in cow’s milk and to consider them from a public health perspective. Fourteen Holstein cows were subjected to milk and blood sampling on the following days in lactation (DIL): 10, 30, 60, 90, 150, 180, 210, 250 and 280 to determine milk hormones, fat and protein content and blood biochemical parameters. For the same purpose, bulk-tank milk was sampled and samples of retail m ilk with 1.5% (CM1.5) and 3.2% (CM3.2) fat was purchased. Milk insulin-like growth factor-1 (IGF-1) values were signifi cantly lower at 90, 150, 180, 210 and 250 and signifi cantly higher at 10, 30 and 60 DIL than lactation average (LA). Milk insulin concentrations were signifi cantly lower at 30, 60 and 90 and higher at 210, 250 and 280 DIL than LA. Free thyroxine (fT4) level in the milk was higher at 250 DIL, while milk free triiodothyronine (fT3) concentrations were lower at 30, 60, 90 and 280 DIL, and signifi cantly higher at 10 and 180 DIL than respective LA. Milk cortisol levels were lower at 60 and 280 DIL than LA. All measured milk hormones were signifi cantly lower in CM1.5 compared to CM3.2, bulk-tank milk and LA. An exception was the LA of IGF-1, which was signifi cantly lower than the IGF1 content in CM1.5. Blood biochemical parameters fl uctuated evenly during lactation and were within the reference range. Hormone concentrations in cow’s milk fl uctuate during lactation, giving milk an important role in the context of public health., Istraživanje je imalo za cilj određivanje koncentracije hormona u mleku krava u različitim fazama laktacije, mleku iz laktofriza i maloprodajnom mleku. Uzorci mleka i krvi prikupljeni su 10., 30., 60., 90., 150., 180., 210., 250. i 280 dana laktacije od četrnaest krava holštajn rase radi određivanja koncentracije hormona, masti i proteina u mleku i biohemijskih parametara krvi. U iste svrhe uzorkovano je mleko iz laktofriza i nabavljeno je komercijalno mleko sa 1,5% (CM1,5) i 3,2% (CM3,2) mlečne masti. Koncentracije insulinu-sličnog faktora rasta-1 (IGF-1) u mleku bile su značajno niže 90., 150., 180., 210. i 250. dana i značajno više 10., 30. i 60. dana laktacije poredeći sa laktacionim prosekom (LP). Insulin je imao značajno niže koncentracije u mleku 30., 60. i 90. dana i značajno više koncentracije 210., 250. i 280. dana laktacije poredeći sa LP. Koncentracija slobodnog tiroksina (fT4) je bila viša 250. dana, dok je nivo slobodnog trijodotironina (fT3) bio značajno niži 30., 60., 90. i 280. dana i značajno viši 10. i 180. dana u poređenju sa odgovarajućim LP. Nivo kortizola u mleku je bio niži 60. i 280. dana laktacije poredeći sa LP. Svi hormoni su imali značajno niže koncentracije u CM1,5 nego u CM3,2, mleku iz laktofriza i LP. Izuzetak je LP za IGF-1, koji je bio značajno niži nego nivo IGF-1 u CM1,5. Biohemijski parametri krvi su blago fl uktuirali tokom laktacije, ali su ostali u referentnom opsegu. Koncentracije hormona u kravljem mleku fl uktuiraju tokom laktacije, što daje značaj mleku u kontekstu javnog zdravlja.",
journal = "Acta Veterinaria",
title = "Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers, Hormoni u sirovom i maloprodajnom mleku krava i potencijalni rizik po zdravlje potrošača",
volume = "74",
number = "1",
pages = "1-16",
doi = "10.2478/acve-2024-0001"
}

Knežević, D., Bošnjaković, D., Dražić, S., Nedić, S., Vujanac, I., Valčić, O., Pantelić, M., Stojiljković, M., Sladojević, Ž.,& Kirovski, D.. (2024). Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers. in Acta Veterinaria, 74(1), 1-16.
https://doi.org/10.2478/acve-2024-0001

Knežević D, Bošnjaković D, Dražić S, Nedić S, Vujanac I, Valčić O, Pantelić M, Stojiljković M, Sladojević Ž, Kirovski D. Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers. in Acta Veterinaria. 2024;74(1):1-16.
doi:10.2478/acve-2024-0001 .

Knežević, Dragan, Bošnjaković, Dušan, Dražić, Slavica, Nedić, Sreten, Vujanac, Ivan, Valčić, Olivera, Pantelić, Marija, Stojiljković, Mojca, Sladojević, Željko, Kirovski, Danijela, "Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers" in Acta Veterinaria, 74, no. 1 (2024):1-16,
https://doi.org/10.2478/acve-2024-0001 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Đorđević, Ana D.
AU  - Romić, Snježana Đ.
AU  - Pantelić, Marija
AU  - Korićanac, Goran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10356
AB  - A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance, though it is evident that dry cow muscles may contain increased triglyceride content. The current study was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ), glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows. Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin 1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to this disposition.
T2  - Research in Veterinary Science
T1  - Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle
VL  - 150
SP  - 189
EP  - 194
DO  - 10.1016/j.rvsc.2022.04.012
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Vujanac, Ivan and Đorđević, Ana D. and Romić, Snježana Đ. and Pantelić, Marija and Korićanac, Goran",
year = "2022",
abstract = "A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance, though it is evident that dry cow muscles may contain increased triglyceride content. The current study was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ), glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows. Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin 1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to this disposition.",
journal = "Research in Veterinary Science",
title = "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle",
volume = "150",
pages = "189-194",
doi = "10.1016/j.rvsc.2022.04.012"
}

Prodanović, R., Kirovski, D., Vujanac, I., Đorđević, A. D., Romić, S. Đ., Pantelić, M.,& Korićanac, G.. (2022). Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science, 150, 189-194.
https://doi.org/10.1016/j.rvsc.2022.04.012

Prodanović R, Kirovski D, Vujanac I, Đorđević AD, Romić SĐ, Pantelić M, Korićanac G. Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science. 2022;150:189-194.
doi:10.1016/j.rvsc.2022.04.012 .

Prodanović, Radiša, Kirovski, Danijela, Vujanac, Ivan, Đorđević, Ana D., Romić, Snježana Đ., Pantelić, Marija, Korićanac, Goran, "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle" in Research in Veterinary Science, 150 (2022):189-194,
https://doi.org/10.1016/j.rvsc.2022.04.012 . .

TY  - JOUR
AU  - Pantelić, Marija
AU  - Jovanović, Ljubomir J.
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Ćulafić, Tijana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jpn.12655
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7804
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 

@article{
author = "Pantelić, Marija and Jovanović, Ljubomir J. and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Ćulafić, Tijana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}

Pantelić, M., Jovanović, L. J., Prodanović, R., Vujanac, I., Đurić, M., Ćulafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition, 102(1), 41-55.
https://doi.org/10.1111/jpn.12655

Pantelić M, Jovanović LJ, Prodanović R, Vujanac I, Đurić M, Ćulafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55.
doi:10.1111/jpn.12655 .

Pantelić, Marija, Jovanović, Ljubomir J., Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Ćulafić, Tijana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" in Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 . .

TY  - JOUR
AU  - Pantelić, Marija
AU  - Jovanović, Ljubomir J.
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Ćulafić, Tijana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jpn.12655
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7835
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. © 2017 Blackwell Verlag GmbH
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 

@article{
author = "Pantelić, Marija and Jovanović, Ljubomir J. and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Ćulafić, Tijana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. © 2017 Blackwell Verlag GmbH",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}

Pantelić, M., Jovanović, L. J., Prodanović, R., Vujanac, I., Đurić, M., Ćulafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition, 102(1), 41-55.
https://doi.org/10.1111/jpn.12655

Pantelić M, Jovanović LJ, Prodanović R, Vujanac I, Đurić M, Ćulafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55.
doi:10.1111/jpn.12655 .

Pantelić, Marija, Jovanović, Ljubomir J., Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Ćulafić, Tijana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" in Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 . .

TY  - JOUR
AU  - Jovanović, Ljubomir J.
AU  - Pantelić, Marija
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Tepavčević, Snežana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1808
AB  - The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.
T2  - Biological Trace Element Research
T1  - Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves
VL  - 180
IS  - 2
SP  - 223
EP  - 232
DO  - 10.1007/s12011-017-1007-1
ER  - 

@article{
author = "Jovanović, Ljubomir J. and Pantelić, Marija and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Tepavčević, Snežana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2017",
abstract = "The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.",
journal = "Biological Trace Element Research",
title = "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves",
volume = "180",
number = "2",
pages = "223-232",
doi = "10.1007/s12011-017-1007-1"
}

Jovanović, L. J., Pantelić, M., Prodanović, R., Vujanac, I., Đurić, M., Tepavčević, S., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2017). Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. in Biological Trace Element Research, 180(2), 223-232.
https://doi.org/10.1007/s12011-017-1007-1

Jovanović LJ, Pantelić M, Prodanović R, Vujanac I, Đurić M, Tepavčević S, Vranješ-Đurić S, Korićanac G, Kirovski D. Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. in Biological Trace Element Research. 2017;180(2):223-232.
doi:10.1007/s12011-017-1007-1 .

Jovanović, Ljubomir J., Pantelić, Marija, Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Tepavčević, Snežana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves" in Biological Trace Element Research, 180, no. 2 (2017):223-232,
https://doi.org/10.1007/s12011-017-1007-1 . .

TY  - JOUR
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Bojkovski, Jovan
AU  - Simeunović, Predrag
AU  - Palamarevic, Milija
AU  - Nedić, Sreten
AU  - Celeska, Irena
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1366
AB  - This study aimed to evaluate glucose-induced insulin response in cows exposed to different temperature-humidity index. Twenty early lactating Holstein-Friesian cows were divided into 2 equal groups based on season, as summer (SU) and spring (SP). SP cows were not exposed to heat stress, while SU cows were exposed to moderate or severe heat stress. Milk production was recorded daily. Starting from day 30 of lactation, intravenous glucose tolerance test (IVGTT) was carried out three times at 30-day intervals. Blood samples were taken before (basal) and after glucose infusion, and glucose and insulin were measured at each sample point. The homeostatic model assessment (HOMA) index was calculated. Milk yield from days 30 to 40 and 64 to 90 of lactation were higher in SP cows than in SU cows. Basal glucose did not differ on days 30 and 60 of lactation, while basal insulin and HOMA were lower in SU compared to SP cows. On day 90 of lactation, SU cows had higher basal glucose, whereas basal insulin and HOMA did not differ. IVGTT results revealed that glucose tolerance was affected by heat stress such that SU cows had higher glucose clearance. Insulin responses to IVGTT did not differ on days 30 and 60 of lactation. Heat stress had a marked effect on insulin secretion on day 90 of lactation, illustrated by higher increments, peak concentrations and area under the curve for insulin in SU cows. Overall, season differences in glucose tolerance depend not only on heat stress and milk production but also on the stage of lactation.
T2  - Acta Veterinaria, Beograd
T1  - Field Trial on Glucose-Induced Insulin Response in High-Yielding Dairy Cows under Different Environmental Temperatures
VL  - 67
IS  - 3
SP  - 366
EP  - 382
DO  - 10.1515/acve-2017-0030
ER  - 

@article{
author = "Vujanac, Ivan and Prodanović, Radiša and Korićanac, Goran and Bojkovski, Jovan and Simeunović, Predrag and Palamarevic, Milija and Nedić, Sreten and Celeska, Irena and Kirovski, Danijela",
year = "2017",
abstract = "This study aimed to evaluate glucose-induced insulin response in cows exposed to different temperature-humidity index. Twenty early lactating Holstein-Friesian cows were divided into 2 equal groups based on season, as summer (SU) and spring (SP). SP cows were not exposed to heat stress, while SU cows were exposed to moderate or severe heat stress. Milk production was recorded daily. Starting from day 30 of lactation, intravenous glucose tolerance test (IVGTT) was carried out three times at 30-day intervals. Blood samples were taken before (basal) and after glucose infusion, and glucose and insulin were measured at each sample point. The homeostatic model assessment (HOMA) index was calculated. Milk yield from days 30 to 40 and 64 to 90 of lactation were higher in SP cows than in SU cows. Basal glucose did not differ on days 30 and 60 of lactation, while basal insulin and HOMA were lower in SU compared to SP cows. On day 90 of lactation, SU cows had higher basal glucose, whereas basal insulin and HOMA did not differ. IVGTT results revealed that glucose tolerance was affected by heat stress such that SU cows had higher glucose clearance. Insulin responses to IVGTT did not differ on days 30 and 60 of lactation. Heat stress had a marked effect on insulin secretion on day 90 of lactation, illustrated by higher increments, peak concentrations and area under the curve for insulin in SU cows. Overall, season differences in glucose tolerance depend not only on heat stress and milk production but also on the stage of lactation.",
journal = "Acta Veterinaria, Beograd",
title = "Field Trial on Glucose-Induced Insulin Response in High-Yielding Dairy Cows under Different Environmental Temperatures",
volume = "67",
number = "3",
pages = "366-382",
doi = "10.1515/acve-2017-0030"
}

Vujanac, I., Prodanović, R., Korićanac, G., Bojkovski, J., Simeunović, P., Palamarevic, M., Nedić, S., Celeska, I.,& Kirovski, D.. (2017). Field Trial on Glucose-Induced Insulin Response in High-Yielding Dairy Cows under Different Environmental Temperatures. in Acta Veterinaria, Beograd, 67(3), 366-382.
https://doi.org/10.1515/acve-2017-0030

Vujanac I, Prodanović R, Korićanac G, Bojkovski J, Simeunović P, Palamarevic M, Nedić S, Celeska I, Kirovski D. Field Trial on Glucose-Induced Insulin Response in High-Yielding Dairy Cows under Different Environmental Temperatures. in Acta Veterinaria, Beograd. 2017;67(3):366-382.
doi:10.1515/acve-2017-0030 .

Vujanac, Ivan, Prodanović, Radiša, Korićanac, Goran, Bojkovski, Jovan, Simeunović, Predrag, Palamarevic, Milija, Nedić, Sreten, Celeska, Irena, Kirovski, Danijela, "Field Trial on Glucose-Induced Insulin Response in High-Yielding Dairy Cows under Different Environmental Temperatures" in Acta Veterinaria, Beograd, 67, no. 3 (2017):366-382,
https://doi.org/10.1515/acve-2017-0030 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Vujanac, Ivan
AU  - Đorđević, Ana D.
AU  - Pantelić, Marija
AU  - Romić, Snježana Đ.
AU  - Stanimirovic, Zoran
AU  - Kirovski, Danijela
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1211
AB  - We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 LT = BCS LT = 3.5) and high (4.0 LT = BCS LT = 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Research in Veterinary Science
T1  - Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
VL  - 107
SP  - 16
EP  - 19
DO  - 10.1016/j.rvsc.2016.04.007
ER  - 

@article{
author = "Prodanović, Radiša and Korićanac, Goran and Vujanac, Ivan and Đorđević, Ana D. and Pantelić, Marija and Romić, Snježana Đ. and Stanimirovic, Zoran and Kirovski, Danijela",
year = "2016",
abstract = "We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 LT = BCS LT = 3.5) and high (4.0 LT = BCS LT = 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Research in Veterinary Science",
title = "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression",
volume = "107",
pages = "16-19",
doi = "10.1016/j.rvsc.2016.04.007"
}

Prodanović, R., Korićanac, G., Vujanac, I., Đorđević, A. D., Pantelić, M., Romić, S. Đ., Stanimirovic, Z.,& Kirovski, D.. (2016). Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science, 107, 16-19.
https://doi.org/10.1016/j.rvsc.2016.04.007

Prodanović R, Korićanac G, Vujanac I, Đorđević AD, Pantelić M, Romić SĐ, Stanimirovic Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science. 2016;107:16-19.
doi:10.1016/j.rvsc.2016.04.007 .

Prodanović, Radiša, Korićanac, Goran, Vujanac, Ivan, Đorđević, Ana D., Pantelić, Marija, Romić, Snježana Đ., Stanimirovic, Zoran, Kirovski, Danijela, "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression" in Research in Veterinary Science, 107 (2016):16-19,
https://doi.org/10.1016/j.rvsc.2016.04.007 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Korićanac, Goran
AU  - Vranješ-Đurić, Sanja
AU  - Ignjatović, Marija
AU  - Samanc, H.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5701
AB  - The aim of this study was to compare insulin responsevness to acute glucose infusion in cows of Holstein Friesian (HF) and Busa breeds during the peripartal period. Eight cows per each group (HF and Busa), were chosen. At day 7 prior to calving (ante partum) and day 14 after calving (post partum) animals were subjected to a glucose tolerance test (GTT). Blood samples were taken immediately before infusion and 15, 30, 60, 120 and 180 min thereafter Glucose and insulin concentrations were measured in each blood sample, while BHBA and NEFA were measured only in samples taken before the infusion. QUICKY an indicator of insulin resistance in cows was calculated. Basal glycemia did not significantly differ between the breeds. Basal insulinemia was significantly higher in Buga than in HF cows in both examined periods (p LT 0.001, respectively). Basal NEFA levels tended (p =0.06) to be higher in Buga cows compared with those of HF ante partum, and was significantly higher (p LT 0.001) post partum. Basal BHBA was significantly lower in Buga than HF cows in both examined periods (p LT 0.01; p LT 0.001). QUICKI was significantly lower in Buga compared to HF cows both ante partum and post partum periods (p LT 0.001, respectively). Glycemia determined during GTT were higher in Buga than HF cows, both ante partum and post partum, but significantly starting from minute 15 ante partum Le. minute 30 post partum. Insulinemia determined during GTT was significantly lower at min 15, and significantly higher starting from min 90 in Buga than HF cows, both ante partum and postpartum. Results obtained in this study indicate on difference in insulin responsevness to acute glucose infusion between the examined breeds, which is probably a consequence of the difference in the degree of negative energy balance rather than of selection on high milk production. Namely, decreased insulin tissues sensitivity and decreased insulin responsiveness in Buga compared to HF cows is probably the consequence of inadequate energy intake from alimentary sources which leads to enhanced usage of energy from body reserves.
T2  - Acta Veterinaria, Beograd
T1  - Insulin Responses to Acute Glucose Infusions in Busa and Holstein-Friesian Cattle Breed During the Peripartum Period: Comparative Study
VL  - 63
IS  - 4
SP  - 373
EP  - 384
DO  - 10.2298/AVB1304373P
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Vujanac, Ivan and Đurić, Miloje and Korićanac, Goran and Vranješ-Đurić, Sanja and Ignjatović, Marija and Samanc, H.",
year = "2013",
abstract = "The aim of this study was to compare insulin responsevness to acute glucose infusion in cows of Holstein Friesian (HF) and Busa breeds during the peripartal period. Eight cows per each group (HF and Busa), were chosen. At day 7 prior to calving (ante partum) and day 14 after calving (post partum) animals were subjected to a glucose tolerance test (GTT). Blood samples were taken immediately before infusion and 15, 30, 60, 120 and 180 min thereafter Glucose and insulin concentrations were measured in each blood sample, while BHBA and NEFA were measured only in samples taken before the infusion. QUICKY an indicator of insulin resistance in cows was calculated. Basal glycemia did not significantly differ between the breeds. Basal insulinemia was significantly higher in Buga than in HF cows in both examined periods (p LT 0.001, respectively). Basal NEFA levels tended (p =0.06) to be higher in Buga cows compared with those of HF ante partum, and was significantly higher (p LT 0.001) post partum. Basal BHBA was significantly lower in Buga than HF cows in both examined periods (p LT 0.01; p LT 0.001). QUICKI was significantly lower in Buga compared to HF cows both ante partum and post partum periods (p LT 0.001, respectively). Glycemia determined during GTT were higher in Buga than HF cows, both ante partum and post partum, but significantly starting from minute 15 ante partum Le. minute 30 post partum. Insulinemia determined during GTT was significantly lower at min 15, and significantly higher starting from min 90 in Buga than HF cows, both ante partum and postpartum. Results obtained in this study indicate on difference in insulin responsevness to acute glucose infusion between the examined breeds, which is probably a consequence of the difference in the degree of negative energy balance rather than of selection on high milk production. Namely, decreased insulin tissues sensitivity and decreased insulin responsiveness in Buga compared to HF cows is probably the consequence of inadequate energy intake from alimentary sources which leads to enhanced usage of energy from body reserves.",
journal = "Acta Veterinaria, Beograd",
title = "Insulin Responses to Acute Glucose Infusions in Busa and Holstein-Friesian Cattle Breed During the Peripartum Period: Comparative Study",
volume = "63",
number = "4",
pages = "373-384",
doi = "10.2298/AVB1304373P"
}

Prodanović, R., Kirovski, D., Vujanac, I., Đurić, M., Korićanac, G., Vranješ-Đurić, S., Ignjatović, M.,& Samanc, H.. (2013). Insulin Responses to Acute Glucose Infusions in Busa and Holstein-Friesian Cattle Breed During the Peripartum Period: Comparative Study. in Acta Veterinaria, Beograd, 63(4), 373-384.
https://doi.org/10.2298/AVB1304373P

Prodanović R, Kirovski D, Vujanac I, Đurić M, Korićanac G, Vranješ-Đurić S, Ignjatović M, Samanc H. Insulin Responses to Acute Glucose Infusions in Busa and Holstein-Friesian Cattle Breed During the Peripartum Period: Comparative Study. in Acta Veterinaria, Beograd. 2013;63(4):373-384.
doi:10.2298/AVB1304373P .

Prodanović, Radiša, Kirovski, Danijela, Vujanac, Ivan, Đurić, Miloje, Korićanac, Goran, Vranješ-Đurić, Sanja, Ignjatović, Marija, Samanc, H., "Insulin Responses to Acute Glucose Infusions in Busa and Holstein-Friesian Cattle Breed During the Peripartum Period: Comparative Study" in Acta Veterinaria, Beograd, 63, no. 4 (2013):373-384,
https://doi.org/10.2298/AVB1304373P . .

TY  - JOUR
AU  - Vujanac, Ivan
AU  - Kirovski, Danijela
AU  - Šamanc, Horea
AU  - Prodanović, Radiša
AU  - Adamović, Milan
AU  - Ignjatović, Marija
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11247
AB  - The objective of this work was to examine the effect of heat stress on glucose and pH values in blood of high-yielding dairy cows in the early stage of lactation, as well as to determine whether the changes in these parameters are interdependent under such conditions. An experiment was performed on high-yielding dairy cows during the summer and the spring periods. Forty cows were selected, twenty each for the two periods under investigation. In the course of the experiment, the temperature humidity index (THI) was determined for the entire period of investigations, and then also the average daily THI, nightmorning THI (average value of hourly THI measured from 22h on the previous day until 10h of the current day), as well as the day-night THI (average value of hourly THI measured during the period from 10h to 22h of the current day). The pH and glucose concentration were determined in blood samples taken in the morning and afternoon of days 30, 60, and 90 of lactation during the spring and summer periods of the investigations. Based on the results for the THI, it was established that the animals were not exposed to the effect of extreme heat stress during the spring period of investigations, while they were periodically exposed to moderate but also extreme heat stress during the summer, in particular in the afternoon hours. It can be concluded from the results obtained for the blood pH that the cows were in respiratory alkalosis during the summer in the morning and afternoon hours on day 30, in the afternoon hours of days 60 and 90 of lactation, as well as in the afternoon on day 90 of lactation during the spring period of investigations. During the summer period, there were no statistically significant differences between the pH value determined in the morning and afternoon hours on day 30 of lactation, while the pH value was significantly higher in the afternoon hours than in the morning hours on days 60 and 90 of lactation. There were no significant differences between the average values for glucose during the spring period of investigations. During the summer, the average afternoon values for glucose on days 60 and 90 of lactation were significantly lower against the morning values. Glucose concentration measured on day 90 was statistically significantly lower than those for days 30 and 60 of lactation, both in the morning and in the afternoon. Glucose concentration on day 90 of lactation during the summer period was significantly lower against glucose concentration during the spring, both in the morning and in the afternoon hours. During the spring period, there was no significant correlation between blood glucemia and pH, while there was a negative correlation between the electrochemical reaction of blood and glucemia during the summer, but which was significant only in the afternoon hours. The decrease in glucemia in the afternoon hours of days 60 and 90 of lactation during the summer period can be explained by the strong influence of alkalosis on insulin secretion that leads to the increased utilization of glucose in peripheral tissues, which is why its concentration in blood is significantly decreased at that time. This is a form of the metabolism shifting under conditions of heat stress, when it uses glucose predominantly as a source of energy in the cells, as its oxidation releases significantly less heat energy in comparison with the combustion of other metabolites.
AB  - Cilj ovog istraživanja je bio da se ispita uticaj toplotnog stresa na vrednosti glikemije i pH krvi kod visokomlečnih krava u ranoj fazi laktacije, kao i da se utvrdi da li su promene ovih parametara u takvim uslovima međusobno zavisne. Ogled je izveden na visokomlečnim kravama tokom leta i proleća. Odabrano je četrdeset krava, po dvadeset za oba perioda ispitivanja. Tokom ogleda računati su satni toplotni indeksi (THI) za ceo period ispitivanja, a zatim i prosečan dnevni THI, noćnojutarnji THI (prosečna vrednost satnih THI izmerenih u periodu od 22h prethodnog dana do 10h ujutro tekućeg dana), kao i dnevnovečernji THI (prosečna vrednost satnih THI izmerenih u periodu od 10 h do 22 h tekućeg dana). U uzorcima krvi uzetim ujutru i posle podne 30, 60. i 90. dana laktacije tokom prolećnog i letnjeg perioda ispitivanja određivan je pH i koncentracija glukoze. Na osnovu rezultata za THI utvrđeno je da životinje u prolećnom periodu ispitivanja nisu bile izložene delovanju izrazitog toplotnog stresa, dok su tokom leta bile periodič no izložene umerenom, ali i izrazitom toplotnom stresu, posebno u poslepodnevnim satima. Iz dobijenih rezultata za pH krvi se može zaključiti da su krave tokom leta bile u respiratornoj alkalozi 30. dana u jutarnjim i popodnevnim, a 60. i 90. dana laktacije u popodnevnim satima, kao i 90. dana laktacije posle podne tokom prolećnog perioda ispitivanja. Tokom leta nije bilo statistički značajne razlike između pH vrednosti određene u jutarnjim i popodnevnim satima 30. dana laktacije, dok je 60. i 90. dana laktacije popodnevna vrednost za pH bila značajno veća u odnosu na jutarnju. Nije bilo značajnih razlika između prosečnih vrednosti glikemija tokom prolećnog perioda ispitivanja. Tokom leta prosečne popodnevne vrednosti glikemija 60. i 90. dana laktacije bile su značajno niže u odnosu na jutarnje. Glikemije izmerene 90. dana bile su statistički značajno niže od glikemija 30. i 60. dana laktacije, kako jutarnje tako i poslepodnevne. Glikemija je 90. dana laktacije u letnjem periodu bila značajno niža u odnosu na glikemiju tokom proleća, kako u jutarnjim, tako i u popodnevnim satima. Tokom proleć nog perioda nije bilo značajne korelacije između glikemije i pH krvi, dok je tokom leta postojala negativna korelacija između elektrohemijske reakcije krvi i glikemije, a koja je bila značajna samo u popodnevnim satima. Smanjenje glikemije u popodnevnim satima 60. i 90. dana laktacije u letnjem periodu može se objasniti snažnim uticajem alkaloze na lučenje insulina, što dovodi do povećanog iskorišćavanja glukoze u perifernim tkivima, zbog čega se njena koncentracija u krvi tada značajno smanjuje. To je vid prestrojavanja metabolizma u uslovima toplotnog stresa kada organizam prevashodno koristi glukozu kao izvor energije u ćelijama jer se njenom oksidacijom oslobađa značajno manje toplotne energije u odnosu na sagorevanje drugih metabolita.
T2  - Veterinarski Glasnik
T1  - Glucose concentration and blood acid-basis status in high-yielding dairy cows during heat stress
T1  - Koncentracija glukoze i acido-bazni status krvi visokomlečnih krava u uslovima toplotnog stresa
VL  - 65
IS  - 5-6
SP  - 297
EP  - 312
DO  - 10.2298/VETGL1106297V
ER  - 

@article{
author = "Vujanac, Ivan and Kirovski, Danijela and Šamanc, Horea and Prodanović, Radiša and Adamović, Milan and Ignjatović, Marija",
year = "2011",
abstract = "The objective of this work was to examine the effect of heat stress on glucose and pH values in blood of high-yielding dairy cows in the early stage of lactation, as well as to determine whether the changes in these parameters are interdependent under such conditions. An experiment was performed on high-yielding dairy cows during the summer and the spring periods. Forty cows were selected, twenty each for the two periods under investigation. In the course of the experiment, the temperature humidity index (THI) was determined for the entire period of investigations, and then also the average daily THI, nightmorning THI (average value of hourly THI measured from 22h on the previous day until 10h of the current day), as well as the day-night THI (average value of hourly THI measured during the period from 10h to 22h of the current day). The pH and glucose concentration were determined in blood samples taken in the morning and afternoon of days 30, 60, and 90 of lactation during the spring and summer periods of the investigations. Based on the results for the THI, it was established that the animals were not exposed to the effect of extreme heat stress during the spring period of investigations, while they were periodically exposed to moderate but also extreme heat stress during the summer, in particular in the afternoon hours. It can be concluded from the results obtained for the blood pH that the cows were in respiratory alkalosis during the summer in the morning and afternoon hours on day 30, in the afternoon hours of days 60 and 90 of lactation, as well as in the afternoon on day 90 of lactation during the spring period of investigations. During the summer period, there were no statistically significant differences between the pH value determined in the morning and afternoon hours on day 30 of lactation, while the pH value was significantly higher in the afternoon hours than in the morning hours on days 60 and 90 of lactation. There were no significant differences between the average values for glucose during the spring period of investigations. During the summer, the average afternoon values for glucose on days 60 and 90 of lactation were significantly lower against the morning values. Glucose concentration measured on day 90 was statistically significantly lower than those for days 30 and 60 of lactation, both in the morning and in the afternoon. Glucose concentration on day 90 of lactation during the summer period was significantly lower against glucose concentration during the spring, both in the morning and in the afternoon hours. During the spring period, there was no significant correlation between blood glucemia and pH, while there was a negative correlation between the electrochemical reaction of blood and glucemia during the summer, but which was significant only in the afternoon hours. The decrease in glucemia in the afternoon hours of days 60 and 90 of lactation during the summer period can be explained by the strong influence of alkalosis on insulin secretion that leads to the increased utilization of glucose in peripheral tissues, which is why its concentration in blood is significantly decreased at that time. This is a form of the metabolism shifting under conditions of heat stress, when it uses glucose predominantly as a source of energy in the cells, as its oxidation releases significantly less heat energy in comparison with the combustion of other metabolites., Cilj ovog istraživanja je bio da se ispita uticaj toplotnog stresa na vrednosti glikemije i pH krvi kod visokomlečnih krava u ranoj fazi laktacije, kao i da se utvrdi da li su promene ovih parametara u takvim uslovima međusobno zavisne. Ogled je izveden na visokomlečnim kravama tokom leta i proleća. Odabrano je četrdeset krava, po dvadeset za oba perioda ispitivanja. Tokom ogleda računati su satni toplotni indeksi (THI) za ceo period ispitivanja, a zatim i prosečan dnevni THI, noćnojutarnji THI (prosečna vrednost satnih THI izmerenih u periodu od 22h prethodnog dana do 10h ujutro tekućeg dana), kao i dnevnovečernji THI (prosečna vrednost satnih THI izmerenih u periodu od 10 h do 22 h tekućeg dana). U uzorcima krvi uzetim ujutru i posle podne 30, 60. i 90. dana laktacije tokom prolećnog i letnjeg perioda ispitivanja određivan je pH i koncentracija glukoze. Na osnovu rezultata za THI utvrđeno je da životinje u prolećnom periodu ispitivanja nisu bile izložene delovanju izrazitog toplotnog stresa, dok su tokom leta bile periodič no izložene umerenom, ali i izrazitom toplotnom stresu, posebno u poslepodnevnim satima. Iz dobijenih rezultata za pH krvi se može zaključiti da su krave tokom leta bile u respiratornoj alkalozi 30. dana u jutarnjim i popodnevnim, a 60. i 90. dana laktacije u popodnevnim satima, kao i 90. dana laktacije posle podne tokom prolećnog perioda ispitivanja. Tokom leta nije bilo statistički značajne razlike između pH vrednosti određene u jutarnjim i popodnevnim satima 30. dana laktacije, dok je 60. i 90. dana laktacije popodnevna vrednost za pH bila značajno veća u odnosu na jutarnju. Nije bilo značajnih razlika između prosečnih vrednosti glikemija tokom prolećnog perioda ispitivanja. Tokom leta prosečne popodnevne vrednosti glikemija 60. i 90. dana laktacije bile su značajno niže u odnosu na jutarnje. Glikemije izmerene 90. dana bile su statistički značajno niže od glikemija 30. i 60. dana laktacije, kako jutarnje tako i poslepodnevne. Glikemija je 90. dana laktacije u letnjem periodu bila značajno niža u odnosu na glikemiju tokom proleća, kako u jutarnjim, tako i u popodnevnim satima. Tokom proleć nog perioda nije bilo značajne korelacije između glikemije i pH krvi, dok je tokom leta postojala negativna korelacija između elektrohemijske reakcije krvi i glikemije, a koja je bila značajna samo u popodnevnim satima. Smanjenje glikemije u popodnevnim satima 60. i 90. dana laktacije u letnjem periodu može se objasniti snažnim uticajem alkaloze na lučenje insulina, što dovodi do povećanog iskorišćavanja glukoze u perifernim tkivima, zbog čega se njena koncentracija u krvi tada značajno smanjuje. To je vid prestrojavanja metabolizma u uslovima toplotnog stresa kada organizam prevashodno koristi glukozu kao izvor energije u ćelijama jer se njenom oksidacijom oslobađa značajno manje toplotne energije u odnosu na sagorevanje drugih metabolita.",
journal = "Veterinarski Glasnik",
title = "Glucose concentration and blood acid-basis status in high-yielding dairy cows during heat stress, Koncentracija glukoze i acido-bazni status krvi visokomlečnih krava u uslovima toplotnog stresa",
volume = "65",
number = "5-6",
pages = "297-312",
doi = "10.2298/VETGL1106297V"
}

Vujanac, I., Kirovski, D., Šamanc, H., Prodanović, R., Adamović, M.,& Ignjatović, M.. (2011). Glucose concentration and blood acid-basis status in high-yielding dairy cows during heat stress. in Veterinarski Glasnik, 65(5-6), 297-312.
https://doi.org/10.2298/VETGL1106297V

Vujanac I, Kirovski D, Šamanc H, Prodanović R, Adamović M, Ignjatović M. Glucose concentration and blood acid-basis status in high-yielding dairy cows during heat stress. in Veterinarski Glasnik. 2011;65(5-6):297-312.
doi:10.2298/VETGL1106297V .

Vujanac, Ivan, Kirovski, Danijela, Šamanc, Horea, Prodanović, Radiša, Adamović, Milan, Ignjatović, Marija, "Glucose concentration and blood acid-basis status in high-yielding dairy cows during heat stress" in Veterinarski Glasnik, 65, no. 5-6 (2011):297-312,
https://doi.org/10.2298/VETGL1106297V . .