TY  - CONF
AU  - Mitrović, Nataša
AU  - Zarić, Marina
AU  - Martinović, Jelena
AU  - Guševac Stojanović, Ivana
AU  - Petrović, Snježana
AU  - Paunović, Marija
AU  - Vučić, Vesna
AU  - Grković, Ivana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11050
AB  - It is increasingly apparent that the prevention/treatment of neurodegenerative disorders is not only achieved through pharmacological therapy but also through the consumption of natural products. Flaxseed oil (or linseed oil, FSO) derived from the seeds of the flax (Linum usitatissimum L.) gained worldwide awareness as a neuroprotective agent due to its high content of omega-3 polyunsaturated fatty acids (n-3 PUFAs). Thus, the aim of this study was to examine the preventive effects of dietary FSO in trimethyltin (TMT) - induced hippocampal neurodegeneration and gliosis in female Wistar rats. Animals were continuously treated with FSO (1 ml/kg, orally) for two weeks, then received a single dose of TMT (8 mg/kg, i.p.), and application of FSO continued for twenty-one days. Data have convincingly shown that FSO continuous treatment ameliorated TMT-induced neuronal loss in the CA3 hippocampal region and ameliorated astrogliosis and microgliosis. FSO treatment elevated all tested n-3 fatty acids in the hippocampus: α-linolenic acid (ALA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), and consequently increased total amount of n-3 PUFA. However, no changes in n-6 fatty acids due to FSO treatment were observed. Consequently, FSO lowered n-6/n-3 ratio compared to TMT, having a protective effect on fatty acid profile in hippocampus. These findings support beneficial neuroprotective properties of FSO against TMT-induced model of neurodegeneration and hint at a promising preventive use of FSO in hippocampal degeneration and dysfunction
PB  - Belgrade : Serbian Neurocardiological Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11050
ER  - 

@conference{
author = "Mitrović, Nataša and Zarić, Marina and Martinović, Jelena and Guševac Stojanović, Ivana and Petrović, Snježana and Paunović, Marija and Vučić, Vesna and Grković, Ivana",
year = "2023",
abstract = "It is increasingly apparent that the prevention/treatment of neurodegenerative disorders is not only achieved through pharmacological therapy but also through the consumption of natural products. Flaxseed oil (or linseed oil, FSO) derived from the seeds of the flax (Linum usitatissimum L.) gained worldwide awareness as a neuroprotective agent due to its high content of omega-3 polyunsaturated fatty acids (n-3 PUFAs). Thus, the aim of this study was to examine the preventive effects of dietary FSO in trimethyltin (TMT) - induced hippocampal neurodegeneration and gliosis in female Wistar rats. Animals were continuously treated with FSO (1 ml/kg, orally) for two weeks, then received a single dose of TMT (8 mg/kg, i.p.), and application of FSO continued for twenty-one days. Data have convincingly shown that FSO continuous treatment ameliorated TMT-induced neuronal loss in the CA3 hippocampal region and ameliorated astrogliosis and microgliosis. FSO treatment elevated all tested n-3 fatty acids in the hippocampus: α-linolenic acid (ALA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), and consequently increased total amount of n-3 PUFA. However, no changes in n-6 fatty acids due to FSO treatment were observed. Consequently, FSO lowered n-6/n-3 ratio compared to TMT, having a protective effect on fatty acid profile in hippocampus. These findings support beneficial neuroprotective properties of FSO against TMT-induced model of neurodegeneration and hint at a promising preventive use of FSO in hippocampal degeneration and dysfunction",
publisher = "Belgrade : Serbian Neurocardiological Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11050"
}

Mitrović, N., Zarić, M., Martinović, J., Guševac Stojanović, I., Petrović, S., Paunović, M., Vučić, V.,& Grković, I.. (2023). Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neurocardiological Society., 81.
https://hdl.handle.net/21.15107/rcub_vinar_11050

Mitrović N, Zarić M, Martinović J, Guševac Stojanović I, Petrović S, Paunović M, Vučić V, Grković I. Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:81.
https://hdl.handle.net/21.15107/rcub_vinar_11050 .

Mitrović, Nataša, Zarić, Marina, Martinović, Jelena, Guševac Stojanović, Ivana, Petrović, Snježana, Paunović, Marija, Vučić, Vesna, Grković, Ivana, "Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):81,
https://hdl.handle.net/21.15107/rcub_vinar_11050 .

TY  - JOUR
AU  - Masnikosa, Romana
AU  - Pirić, David
AU  - Post, Julia Maria
AU  - Cvetković, Zorica
AU  - Petrović, Snježana
AU  - Paunović, Marija
AU  - Vučić, Vesna
AU  - Bindila, Laura
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11437
AB  - Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer.
T2  - Cancers
T1  - Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study
VL  - 15
IS  - 14
SP  - 3653
DO  - 10.3390/cancers15143653
ER  - 

@article{
author = "Masnikosa, Romana and Pirić, David and Post, Julia Maria and Cvetković, Zorica and Petrović, Snježana and Paunović, Marija and Vučić, Vesna and Bindila, Laura",
year = "2023",
abstract = "Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer.",
journal = "Cancers",
title = "Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study",
volume = "15",
number = "14",
pages = "3653",
doi = "10.3390/cancers15143653"
}

Masnikosa, R., Pirić, D., Post, J. M., Cvetković, Z., Petrović, S., Paunović, M., Vučić, V.,& Bindila, L.. (2023). Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study. in Cancers, 15(14), 3653.
https://doi.org/10.3390/cancers15143653

Masnikosa R, Pirić D, Post JM, Cvetković Z, Petrović S, Paunović M, Vučić V, Bindila L. Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study. in Cancers. 2023;15(14):3653.
doi:10.3390/cancers15143653 .

Masnikosa, Romana, Pirić, David, Post, Julia Maria, Cvetković, Zorica, Petrović, Snježana, Paunović, Marija, Vučić, Vesna, Bindila, Laura, "Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study" in Cancers, 15, no. 14 (2023):3653,
https://doi.org/10.3390/cancers15143653 . .

TY  - JOUR
AU  - Resanović, Ivana
AU  - Gluvić, Zoran
AU  - Zarić, Božidarka
AU  - Sudar-Milovanović, Emina
AU  - Vučić, Vesna
AU  - Arsić, Aleksandra
AU  - Nedić, Olgica
AU  - Šunderić, Miloš
AU  - Gligorijević, Nikola
AU  - Milačić, Davorka
AU  - Isenović, Esma R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8567
AB  - Objective: Metabolic changes in insulin-dependent diabetes mellitus (IDDM) impair vasodilation, and this leads to tissue hypoxia and microvascular pathology. Hyperbaric oxygen therapy (HBOT) can significantly improve the outcome of ischemic conditions in IDDM patients and reduce vascular complications. The aim of our study was to assess the effects of HBOT on plasma fatty acid (FA) composition, and expression of insulin-like growth factor binding protein 1 (IGFBP-1) in IDDM patients. Methods: Our study included 24 adult IDDM patients diagnosed with peripheral vascular complications. The patients were exposed to 10 sessions of 100% oxygen inhalation at 2.4 atmosphere absolute for 1 hour. Blood samples were collected at admission and after HBOT for measurement of metabolic parameters, FA composition and IGFBP-1. Measurement of plasma FA composition was determined by gas chromatography. Expression of IGFBP-1 in the serum was estimated by Western blot analysis. Results: HBOT decreased blood levels of total cholesterol (p<0.05), triglycerides (p<0.05) and low-density lipoprotein (p<0.05). HBOT increased plasma levels of individual FAs: palmitic acid (p<0.05), palmitoleic acid (p<0.05), docosapentaenoic acid (p<0.05) and docosahexaenoic acid (p<0.01), and decreased levels of stearic acid (p<0.05), alpha linolenic acid (p<0.05) and linoleic acid (p<0.01). Expression of IGFBP-1 (p<0.01) was increased, whereas the level of insulin (p<0.001) was decreased in the serum after HBOT. Conclusions: Our results indicate that HBOT exerts beneficial effects in IDDM patients by improving the lipid profile and altering FA composition. © 2019 Canadian Diabetes Association
T2  - Canadian Journal of Diabetes
T1  - Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study
VL  - 44
IS  - 1
SP  - 22
EP  - 29
DO  - 10.1016/j.jcjd.2019.04.018
ER  - 

@article{
author = "Resanović, Ivana and Gluvić, Zoran and Zarić, Božidarka and Sudar-Milovanović, Emina and Vučić, Vesna and Arsić, Aleksandra and Nedić, Olgica and Šunderić, Miloš and Gligorijević, Nikola and Milačić, Davorka and Isenović, Esma R.",
year = "2020",
abstract = "Objective: Metabolic changes in insulin-dependent diabetes mellitus (IDDM) impair vasodilation, and this leads to tissue hypoxia and microvascular pathology. Hyperbaric oxygen therapy (HBOT) can significantly improve the outcome of ischemic conditions in IDDM patients and reduce vascular complications. The aim of our study was to assess the effects of HBOT on plasma fatty acid (FA) composition, and expression of insulin-like growth factor binding protein 1 (IGFBP-1) in IDDM patients. Methods: Our study included 24 adult IDDM patients diagnosed with peripheral vascular complications. The patients were exposed to 10 sessions of 100% oxygen inhalation at 2.4 atmosphere absolute for 1 hour. Blood samples were collected at admission and after HBOT for measurement of metabolic parameters, FA composition and IGFBP-1. Measurement of plasma FA composition was determined by gas chromatography. Expression of IGFBP-1 in the serum was estimated by Western blot analysis. Results: HBOT decreased blood levels of total cholesterol (p<0.05), triglycerides (p<0.05) and low-density lipoprotein (p<0.05). HBOT increased plasma levels of individual FAs: palmitic acid (p<0.05), palmitoleic acid (p<0.05), docosapentaenoic acid (p<0.05) and docosahexaenoic acid (p<0.01), and decreased levels of stearic acid (p<0.05), alpha linolenic acid (p<0.05) and linoleic acid (p<0.01). Expression of IGFBP-1 (p<0.01) was increased, whereas the level of insulin (p<0.001) was decreased in the serum after HBOT. Conclusions: Our results indicate that HBOT exerts beneficial effects in IDDM patients by improving the lipid profile and altering FA composition. © 2019 Canadian Diabetes Association",
journal = "Canadian Journal of Diabetes",
title = "Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study",
volume = "44",
number = "1",
pages = "22-29",
doi = "10.1016/j.jcjd.2019.04.018"
}

Resanović, I., Gluvić, Z., Zarić, B., Sudar-Milovanović, E., Vučić, V., Arsić, A., Nedić, O., Šunderić, M., Gligorijević, N., Milačić, D.,& Isenović, E. R.. (2020). Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study. in Canadian Journal of Diabetes, 44(1), 22-29.
https://doi.org/10.1016/j.jcjd.2019.04.018

Resanović I, Gluvić Z, Zarić B, Sudar-Milovanović E, Vučić V, Arsić A, Nedić O, Šunderić M, Gligorijević N, Milačić D, Isenović ER. Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study. in Canadian Journal of Diabetes. 2020;44(1):22-29.
doi:10.1016/j.jcjd.2019.04.018 .

Resanović, Ivana, Gluvić, Zoran, Zarić, Božidarka, Sudar-Milovanović, Emina, Vučić, Vesna, Arsić, Aleksandra, Nedić, Olgica, Šunderić, Miloš, Gligorijević, Nikola, Milačić, Davorka, Isenović, Esma R., "Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study" in Canadian Journal of Diabetes, 44, no. 1 (2020):22-29,
https://doi.org/10.1016/j.jcjd.2019.04.018 . .

TY  - JOUR
AU  - Cvetković, Zorica
AU  - Milošević, Maja
AU  - Cvetković, Bora
AU  - Masnikosa, Romana
AU  - Arsić, Aleksandra
AU  - Petrović, Snježana
AU  - Vučić, Vesna
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1489
AB  - Limited studies have been performed to associate abnormal phospholipid (PL) profile and disease activity in hematological malignancies, including non-Hodgkin lymphoma (NHL). The aim of his study was to evaluate the levels of plasma PL fractions in NHL patients, in response to chemotherapy. Forty non-treated patients with NHL and 25 healthy individuals were recruited. Blood samples from patients were taken before chemotherapy, after 3 cycles and after the end of the treatment, and PL fractions were resolved by one-dimensional thin-layer chromatography. To assess potential relationship between plasma PL profile and response to therapy, patients were divided according to clinical outcome in 3 groups: complete remission (CR), stable disease (SD) and progression (PG). Despite significant differences between NHL patients and healthy controls, no differences were found at baseline among patients divided according to clinical outcome. During and after chemotherapy important alterations in PL profile were observed. Levels of total PLs and all PL fractions decreased in patients with PG while in patients who responded to therapy (CR, SD) PLs significantly increased. Results of our study suggest that changes of total PLs and PL fractions during the therapy are associated with the effects of therapy and clinical outcome in patients with NHL. (C) 2017 Elsevier Ltd. All rights reserved.
T2  - Leukemia Research
T1  - Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients
VL  - 54
SP  - 39
EP  - 46
DO  - 10.1016/j.leukres.2017.01.004
ER  - 

@article{
author = "Cvetković, Zorica and Milošević, Maja and Cvetković, Bora and Masnikosa, Romana and Arsić, Aleksandra and Petrović, Snježana and Vučić, Vesna",
year = "2017",
abstract = "Limited studies have been performed to associate abnormal phospholipid (PL) profile and disease activity in hematological malignancies, including non-Hodgkin lymphoma (NHL). The aim of his study was to evaluate the levels of plasma PL fractions in NHL patients, in response to chemotherapy. Forty non-treated patients with NHL and 25 healthy individuals were recruited. Blood samples from patients were taken before chemotherapy, after 3 cycles and after the end of the treatment, and PL fractions were resolved by one-dimensional thin-layer chromatography. To assess potential relationship between plasma PL profile and response to therapy, patients were divided according to clinical outcome in 3 groups: complete remission (CR), stable disease (SD) and progression (PG). Despite significant differences between NHL patients and healthy controls, no differences were found at baseline among patients divided according to clinical outcome. During and after chemotherapy important alterations in PL profile were observed. Levels of total PLs and all PL fractions decreased in patients with PG while in patients who responded to therapy (CR, SD) PLs significantly increased. Results of our study suggest that changes of total PLs and PL fractions during the therapy are associated with the effects of therapy and clinical outcome in patients with NHL. (C) 2017 Elsevier Ltd. All rights reserved.",
journal = "Leukemia Research",
title = "Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients",
volume = "54",
pages = "39-46",
doi = "10.1016/j.leukres.2017.01.004"
}

Cvetković, Z., Milošević, M., Cvetković, B., Masnikosa, R., Arsić, A., Petrović, S.,& Vučić, V.. (2017). Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients. in Leukemia Research, 54, 39-46.
https://doi.org/10.1016/j.leukres.2017.01.004

Cvetković Z, Milošević M, Cvetković B, Masnikosa R, Arsić A, Petrović S, Vučić V. Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients. in Leukemia Research. 2017;54:39-46.
doi:10.1016/j.leukres.2017.01.004 .

Cvetković, Zorica, Milošević, Maja, Cvetković, Bora, Masnikosa, Romana, Arsić, Aleksandra, Petrović, Snježana, Vučić, Vesna, "Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients" in Leukemia Research, 54 (2017):39-46,
https://doi.org/10.1016/j.leukres.2017.01.004 . .

TY  - JOUR
AU  - Marković, Dejan
AU  - Karadzic, Ivana
AU  - Jokanović, Vukoman R.
AU  - Vukovic, Ana
AU  - Vučić, Vesna
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1258
AB  - Millions of patients worldwide need surgery to repair or replace tissue that has been damaged through trauma or disease. To solve the problem of lost tissue, a major emphasis of tissue engineering (TE) is on tissue regeneration. Stem cells and highly porous biomaterials used as cell carriers (scaffolds) have an essential role in the production of new tissue by TE. The cellular component is important for the generation and establishment of the extracellular matrix, while a scaffold is necessary to determine the shape of the newly formed tissue and facilitate migration of cells into the desired location, as well as their growth and differentiation. This review describes the types, characteristics and classification of stem cells. Furthermore, it includes functional features of cell carriers - biocompatibility, biodegradability and mechanical properties of biomaterials used in developing state-of-the-an scaffolds for TE applications, as well as suitability for different tissues. Moreover, it explains the importance of nanotechnology and defines the challenges and the purpose of future research in this rapidly advancing field.
T2  - Chemical Industry and Chemical Engineering Quarterly / CICEQ
T1  - Biological Aspects of Application of Nanomaterials in Tissue Engineering
VL  - 22
IS  - 2
SP  - 145
EP  - 153
DO  - 10.2298/CICEQ141231028M
ER  - 

@article{
author = "Marković, Dejan and Karadzic, Ivana and Jokanović, Vukoman R. and Vukovic, Ana and Vučić, Vesna",
year = "2016",
abstract = "Millions of patients worldwide need surgery to repair or replace tissue that has been damaged through trauma or disease. To solve the problem of lost tissue, a major emphasis of tissue engineering (TE) is on tissue regeneration. Stem cells and highly porous biomaterials used as cell carriers (scaffolds) have an essential role in the production of new tissue by TE. The cellular component is important for the generation and establishment of the extracellular matrix, while a scaffold is necessary to determine the shape of the newly formed tissue and facilitate migration of cells into the desired location, as well as their growth and differentiation. This review describes the types, characteristics and classification of stem cells. Furthermore, it includes functional features of cell carriers - biocompatibility, biodegradability and mechanical properties of biomaterials used in developing state-of-the-an scaffolds for TE applications, as well as suitability for different tissues. Moreover, it explains the importance of nanotechnology and defines the challenges and the purpose of future research in this rapidly advancing field.",
journal = "Chemical Industry and Chemical Engineering Quarterly / CICEQ",
title = "Biological Aspects of Application of Nanomaterials in Tissue Engineering",
volume = "22",
number = "2",
pages = "145-153",
doi = "10.2298/CICEQ141231028M"
}

Marković, D., Karadzic, I., Jokanović, V. R., Vukovic, A.,& Vučić, V.. (2016). Biological Aspects of Application of Nanomaterials in Tissue Engineering. in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 22(2), 145-153.
https://doi.org/10.2298/CICEQ141231028M

Marković D, Karadzic I, Jokanović VR, Vukovic A, Vučić V. Biological Aspects of Application of Nanomaterials in Tissue Engineering. in Chemical Industry and Chemical Engineering Quarterly / CICEQ. 2016;22(2):145-153.
doi:10.2298/CICEQ141231028M .

Marković, Dejan, Karadzic, Ivana, Jokanović, Vukoman R., Vukovic, Ana, Vučić, Vesna, "Biological Aspects of Application of Nanomaterials in Tissue Engineering" in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 22, no. 2 (2016):145-153,
https://doi.org/10.2298/CICEQ141231028M . .

TY  - JOUR
AU  - Karadzic, Ivana
AU  - Vučić, Vesna
AU  - Jokanović, Vukoman R.
AU  - Debeljak-Martačić, Jasmina
AU  - Marković, Dejan
AU  - Petrović, Snježana
AU  - Glibetić, Marija
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/235
AB  - The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.
T2  - Journal of Biomedical Materials Research. Part A
T1  - Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells
VL  - 103
IS  - 1
SP  - 350
EP  - 357
DO  - 10.1002/jbm.a.35180
ER  - 

@article{
author = "Karadzic, Ivana and Vučić, Vesna and Jokanović, Vukoman R. and Debeljak-Martačić, Jasmina and Marković, Dejan and Petrović, Snježana and Glibetić, Marija",
year = "2015",
abstract = "The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.",
journal = "Journal of Biomedical Materials Research. Part A",
title = "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells",
volume = "103",
number = "1",
pages = "350-357",
doi = "10.1002/jbm.a.35180"
}

Karadzic, I., Vučić, V., Jokanović, V. R., Debeljak-Martačić, J., Marković, D., Petrović, S.,& Glibetić, M.. (2015). Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research. Part A, 103(1), 350-357.
https://doi.org/10.1002/jbm.a.35180

Karadzic I, Vučić V, Jokanović VR, Debeljak-Martačić J, Marković D, Petrović S, Glibetić M. Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research. Part A. 2015;103(1):350-357.
doi:10.1002/jbm.a.35180 .

Karadzic, Ivana, Vučić, Vesna, Jokanović, Vukoman R., Debeljak-Martačić, Jasmina, Marković, Dejan, Petrović, Snježana, Glibetić, Marija, "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells" in Journal of Biomedical Materials Research. Part A, 103, no. 1 (2015):350-357,
https://doi.org/10.1002/jbm.a.35180 . .

TY  - JOUR
AU  - Petrovic, S.
AU  - Takic, M.
AU  - Arsic, A.
AU  - Vučić, Vesna
AU  - Drakulić, Dunja R.
AU  - Milošević, Maja
AU  - Glibetić, Marija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6095
AB  - The effects of 8-days treatment with 17 alpha-estradiol (33.3 mu g/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was found in BCO rats after the estradiol treatment. Both hormones elevated proportion of 18:1n-7 fatty acid in INT, but they failed to have such an effect in BCO. Estradiol increased 22:5n-3 and total n-3 polyunsaturated fatty acids (PUFA) in intact, and decreased 18:2n-6 in BCO rats. Significantly lower level of total n-3 was found in progesterone-treated than in estradiol-treated BCO rats. Given that n-3 PUFA have many beneficial effects on cell and tissue function, while n-6 PUFA have mostly the opposite effects, estradiol, rather than progesterone, was seen to improve plasma lipids and phospholipids FA profiles in INT and BCO animals. Estradiol significantly elevated the estimated activity of Delta 9-desaturases and progesterone of Delta 5-desaturase in BCO group, with no effects in INT rats.
T2  - Physiological Research
T1  - Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries
VL  - 63
IS  - 3
SP  - 331
EP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6095
ER  - 

@article{
author = "Petrovic, S. and Takic, M. and Arsic, A. and Vučić, Vesna and Drakulić, Dunja R. and Milošević, Maja and Glibetić, Marija",
year = "2014",
abstract = "The effects of 8-days treatment with 17 alpha-estradiol (33.3 mu g/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was found in BCO rats after the estradiol treatment. Both hormones elevated proportion of 18:1n-7 fatty acid in INT, but they failed to have such an effect in BCO. Estradiol increased 22:5n-3 and total n-3 polyunsaturated fatty acids (PUFA) in intact, and decreased 18:2n-6 in BCO rats. Significantly lower level of total n-3 was found in progesterone-treated than in estradiol-treated BCO rats. Given that n-3 PUFA have many beneficial effects on cell and tissue function, while n-6 PUFA have mostly the opposite effects, estradiol, rather than progesterone, was seen to improve plasma lipids and phospholipids FA profiles in INT and BCO animals. Estradiol significantly elevated the estimated activity of Delta 9-desaturases and progesterone of Delta 5-desaturase in BCO group, with no effects in INT rats.",
journal = "Physiological Research",
title = "Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries",
volume = "63",
number = "3",
pages = "331-339",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6095"
}

Petrovic, S., Takic, M., Arsic, A., Vučić, V., Drakulić, D. R., Milošević, M.,& Glibetić, M.. (2014). Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries. in Physiological Research, 63(3), 331-339.
https://hdl.handle.net/21.15107/rcub_vinar_6095

Petrovic S, Takic M, Arsic A, Vučić V, Drakulić DR, Milošević M, Glibetić M. Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries. in Physiological Research. 2014;63(3):331-339.
https://hdl.handle.net/21.15107/rcub_vinar_6095 .

Petrovic, S., Takic, M., Arsic, A., Vučić, Vesna, Drakulić, Dunja R., Milošević, Maja, Glibetić, Marija, "Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries" in Physiological Research, 63, no. 3 (2014):331-339,
https://hdl.handle.net/21.15107/rcub_vinar_6095 .

TY  - JOUR
AU  - Vučić, Vesna
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3467
AB  - The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.
T2  - Investigational New Drugs
T1  - The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells
VL  - 26
IS  - 4
SP  - 309
EP  - 317
DO  - 10.1007/s10637-007-9101-4
ER  - 

@article{
author = "Vučić, Vesna and Nićiforović, Ana and Adžić, Miroslav and Radojčić, Marija and Ruždijić, Sabera",
year = "2008",
abstract = "The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.",
journal = "Investigational New Drugs",
title = "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells",
volume = "26",
number = "4",
pages = "309-317",
doi = "10.1007/s10637-007-9101-4"
}

Vučić, V., Nićiforović, A., Adžić, M., Radojčić, M.,& Ruždijić, S.. (2008). The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs, 26(4), 309-317.
https://doi.org/10.1007/s10637-007-9101-4

Vučić V, Nićiforović A, Adžić M, Radojčić M, Ruždijić S. The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs. 2008;26(4):309-317.
doi:10.1007/s10637-007-9101-4 .

Vučić, Vesna, Nićiforović, Ana, Adžić, Miroslav, Radojčić, Marija, Ruždijić, Sabera, "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells" in Investigational New Drugs, 26, no. 4 (2008):309-317,
https://doi.org/10.1007/s10637-007-9101-4 . .

TY  - JOUR
AU  - Nićiforović, Ana
AU  - Đorđević, Jelena D.
AU  - Adžić, Miroslav
AU  - Vučić, Vesna
AU  - Mitrasinovic, Petar M.
AU  - Radojčić, Marija
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3404
AB  - Molecular mechanisms for the gamma-ionizing radiation (IR) resistance of human prostate cancer cells, PC-3, are not quite clear. Since the low-LET-IR effects are primarily manifested by the generation of reactive oxygen species (ROS), the IR-induced expressions both of ROS-metabolizing antioxidant enzymes, such as Mn- and CuZn superoxide dismutases (SODs) and catalase (Cat), and of the transcriptional nuclear factor-kappaB (NF-kappa B) were explored. A substantial increase in the concentrations of SODs was observed in the cells irradiated by 10 and 20 Gy relative to those irradiated by 0 and 2 Gy, while the Cat and NF-kappa B expressions were found to be fairly stable. A system biology model was developed to shed more light on how MnSOD affects the biological state of cells depending upon the production of H2O2. By raising the initial presence of MnSOD in the 0.7-10 mu M concentration range, the time-dependent concentrations of H2O2 for various initial levels of MnSOD were contrasted. The radioresistance of PC-3 cells is suggested to be associated with the positive, feed-forward vicious circle established between the H2O2-mediated elevation of MnSOD expression.
T2  - Annals of Biomedical Engineering
T1  - Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells
VL  - 36
IS  - 5
SP  - 831
EP  - 838
DO  - 10.1007/s10439-008-9457-4
ER  - 

@article{
author = "Nićiforović, Ana and Đorđević, Jelena D. and Adžić, Miroslav and Vučić, Vesna and Mitrasinovic, Petar M. and Radojčić, Marija",
year = "2008",
abstract = "Molecular mechanisms for the gamma-ionizing radiation (IR) resistance of human prostate cancer cells, PC-3, are not quite clear. Since the low-LET-IR effects are primarily manifested by the generation of reactive oxygen species (ROS), the IR-induced expressions both of ROS-metabolizing antioxidant enzymes, such as Mn- and CuZn superoxide dismutases (SODs) and catalase (Cat), and of the transcriptional nuclear factor-kappaB (NF-kappa B) were explored. A substantial increase in the concentrations of SODs was observed in the cells irradiated by 10 and 20 Gy relative to those irradiated by 0 and 2 Gy, while the Cat and NF-kappa B expressions were found to be fairly stable. A system biology model was developed to shed more light on how MnSOD affects the biological state of cells depending upon the production of H2O2. By raising the initial presence of MnSOD in the 0.7-10 mu M concentration range, the time-dependent concentrations of H2O2 for various initial levels of MnSOD were contrasted. The radioresistance of PC-3 cells is suggested to be associated with the positive, feed-forward vicious circle established between the H2O2-mediated elevation of MnSOD expression.",
journal = "Annals of Biomedical Engineering",
title = "Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells",
volume = "36",
number = "5",
pages = "831-838",
doi = "10.1007/s10439-008-9457-4"
}

Nićiforović, A., Đorđević, J. D., Adžić, M., Vučić, V., Mitrasinovic, P. M.,& Radojčić, M.. (2008). Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells. in Annals of Biomedical Engineering, 36(5), 831-838.
https://doi.org/10.1007/s10439-008-9457-4

Nićiforović A, Đorđević JD, Adžić M, Vučić V, Mitrasinovic PM, Radojčić M. Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells. in Annals of Biomedical Engineering. 2008;36(5):831-838.
doi:10.1007/s10439-008-9457-4 .

Nićiforović, Ana, Đorđević, Jelena D., Adžić, Miroslav, Vučić, Vesna, Mitrasinovic, Petar M., Radojčić, Marija, "Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells" in Annals of Biomedical Engineering, 36, no. 5 (2008):831-838,
https://doi.org/10.1007/s10439-008-9457-4 . .

TY  - CONF
AU  - Nićiforović, Ana
AU  - Đorđević, Jelena D.
AU  - Adžić, Miroslav
AU  - Vučić, Vesna
AU  - Mitrasinovic, Petar M.
AU  - Radojčić, Marija
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6410
C3  - Abstracts of Papers of the American Chemical Society
T1  - MEDI 314-Experimental and systems biology studies of the radioresistance of prostate carcinoma cells
VL  - 234
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6410
ER  - 

@conference{
author = "Nićiforović, Ana and Đorđević, Jelena D. and Adžić, Miroslav and Vučić, Vesna and Mitrasinovic, Petar M. and Radojčić, Marija",
year = "2007",
journal = "Abstracts of Papers of the American Chemical Society",
title = "MEDI 314-Experimental and systems biology studies of the radioresistance of prostate carcinoma cells",
volume = "234",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6410"
}

Nićiforović, A., Đorđević, J. D., Adžić, M., Vučić, V., Mitrasinovic, P. M.,& Radojčić, M.. (2007). MEDI 314-Experimental and systems biology studies of the radioresistance of prostate carcinoma cells. in Abstracts of Papers of the American Chemical Society, 234.
https://hdl.handle.net/21.15107/rcub_vinar_6410

Nićiforović A, Đorđević JD, Adžić M, Vučić V, Mitrasinovic PM, Radojčić M. MEDI 314-Experimental and systems biology studies of the radioresistance of prostate carcinoma cells. in Abstracts of Papers of the American Chemical Society. 2007;234.
https://hdl.handle.net/21.15107/rcub_vinar_6410 .

Nićiforović, Ana, Đorđević, Jelena D., Adžić, Miroslav, Vučić, Vesna, Mitrasinovic, Petar M., Radojčić, Marija, "MEDI 314-Experimental and systems biology studies of the radioresistance of prostate carcinoma cells" in Abstracts of Papers of the American Chemical Society, 234 (2007),
https://hdl.handle.net/21.15107/rcub_vinar_6410 .

TY  - CONF
AU  - Nićiforović, Ana
AU  - Đorđević, Jelena D.
AU  - Adžić, Miroslav
AU  - Vučić, Vesna
AU  - Mitrasinovic, Petar M.
AU  - Radojčić, Marija
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6409
C3  - Abstracts of Papers of the American Chemical Society
T1  - BIOL 165-A kinetic study of the radioresistance of prostate carcinoma cells
VL  - 234
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6409
ER  - 

@conference{
author = "Nićiforović, Ana and Đorđević, Jelena D. and Adžić, Miroslav and Vučić, Vesna and Mitrasinovic, Petar M. and Radojčić, Marija",
year = "2007",
journal = "Abstracts of Papers of the American Chemical Society",
title = "BIOL 165-A kinetic study of the radioresistance of prostate carcinoma cells",
volume = "234",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6409"
}

Nićiforović, A., Đorđević, J. D., Adžić, M., Vučić, V., Mitrasinovic, P. M.,& Radojčić, M.. (2007). BIOL 165-A kinetic study of the radioresistance of prostate carcinoma cells. in Abstracts of Papers of the American Chemical Society, 234.
https://hdl.handle.net/21.15107/rcub_vinar_6409

Nićiforović A, Đorđević JD, Adžić M, Vučić V, Mitrasinovic PM, Radojčić M. BIOL 165-A kinetic study of the radioresistance of prostate carcinoma cells. in Abstracts of Papers of the American Chemical Society. 2007;234.
https://hdl.handle.net/21.15107/rcub_vinar_6409 .

Nićiforović, Ana, Đorđević, Jelena D., Adžić, Miroslav, Vučić, Vesna, Mitrasinovic, Petar M., Radojčić, Marija, "BIOL 165-A kinetic study of the radioresistance of prostate carcinoma cells" in Abstracts of Papers of the American Chemical Society, 234 (2007),
https://hdl.handle.net/21.15107/rcub_vinar_6409 .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Vučić, Vesna
AU  - Nešković-Konstantinović, Zora
AU  - Spasić, Snežana D.
AU  - Jones, David R.
AU  - Radoičić, Marija B.
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3008
AB  - There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappa B and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappa B and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.
T2  - Redox Report
T1  - Systemic NF-κB activation in blood cells of breast cancer patients
VL  - 11
IS  - 1
SP  - 38
EP  - 44
DO  - 10.1179/135100006X101002
ER  - 

@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Vučić, Vesna and Nešković-Konstantinović, Zora and Spasić, Snežana D. and Jones, David R. and Radoičić, Marija B. and Spasić, Mihajlo",
year = "2006",
abstract = "There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappa B and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappa B and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.",
journal = "Redox Report",
title = "Systemic NF-κB activation in blood cells of breast cancer patients",
volume = "11",
number = "1",
pages = "38-44",
doi = "10.1179/135100006X101002"
}

Adžić, M., Nićiforović, A., Vučić, V., Nešković-Konstantinović, Z., Spasić, S. D., Jones, D. R., Radoičić, M. B.,& Spasić, M.. (2006). Systemic NF-κB activation in blood cells of breast cancer patients. in Redox Report, 11(1), 38-44.
https://doi.org/10.1179/135100006X101002

Adžić M, Nićiforović A, Vučić V, Nešković-Konstantinović Z, Spasić SD, Jones DR, Radoičić MB, Spasić M. Systemic NF-κB activation in blood cells of breast cancer patients. in Redox Report. 2006;11(1):38-44.
doi:10.1179/135100006X101002 .

Adžić, Miroslav, Nićiforović, Ana, Vučić, Vesna, Nešković-Konstantinović, Zora, Spasić, Snežana D., Jones, David R., Radoičić, Marija B., Spasić, Mihajlo, "Systemic NF-κB activation in blood cells of breast cancer patients" in Redox Report, 11, no. 1 (2006):38-44,
https://doi.org/10.1179/135100006X101002 . .

TY  - JOUR
AU  - Vučić, Vesna
AU  - Isenović, Esma R.
AU  - Adžić, Miroslav
AU  - Ruždijić, Sabera
AU  - Radojčić, Marija
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2977
AB  - Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of Co-60 gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a significant (P LT 0.001) antiproliferative and cytotoxic effect on DU 145 cells. Both effects were time and dose dependent. Also, the dose of gamma-IR which inhibited DNA synthesis and cell proliferation by 50% was 9.7 Gy. Furthermore, gamma-IR induced cell cycle arrest in the G2/M phase and the percentage of cells in the G2/M phase was increased from 15% (control) to 49% (IR cells), with a nonsignificant induction of apoptosis. Treatment with 10 Gy gamma-IR for 24, 48, and 72 h stimulated CuZnSOD and MnSOD protein expression in a time-dependent manner, approximately by 3- to 3.5-fold. These data suggest that CuZnSOD and MnSOD enzymes may play an important role in the gamma-IR-induced changes in DU 145 cell growth, cell cycle arrest and cell death.
T2  - Brazilian Journal of Medical and Biological Research
T1  - Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells
VL  - 39
IS  - 2
SP  - 227
EP  - 236
DO  - 10.1590/S0100-879X2006000200009
ER  - 

@article{
author = "Vučić, Vesna and Isenović, Esma R. and Adžić, Miroslav and Ruždijić, Sabera and Radojčić, Marija",
year = "2006",
abstract = "Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of Co-60 gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a significant (P LT 0.001) antiproliferative and cytotoxic effect on DU 145 cells. Both effects were time and dose dependent. Also, the dose of gamma-IR which inhibited DNA synthesis and cell proliferation by 50% was 9.7 Gy. Furthermore, gamma-IR induced cell cycle arrest in the G2/M phase and the percentage of cells in the G2/M phase was increased from 15% (control) to 49% (IR cells), with a nonsignificant induction of apoptosis. Treatment with 10 Gy gamma-IR for 24, 48, and 72 h stimulated CuZnSOD and MnSOD protein expression in a time-dependent manner, approximately by 3- to 3.5-fold. These data suggest that CuZnSOD and MnSOD enzymes may play an important role in the gamma-IR-induced changes in DU 145 cell growth, cell cycle arrest and cell death.",
journal = "Brazilian Journal of Medical and Biological Research",
title = "Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells",
volume = "39",
number = "2",
pages = "227-236",
doi = "10.1590/S0100-879X2006000200009"
}

Vučić, V., Isenović, E. R., Adžić, M., Ruždijić, S.,& Radojčić, M.. (2006). Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells. in Brazilian Journal of Medical and Biological Research, 39(2), 227-236.
https://doi.org/10.1590/S0100-879X2006000200009

Vučić V, Isenović ER, Adžić M, Ruždijić S, Radojčić M. Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells. in Brazilian Journal of Medical and Biological Research. 2006;39(2):227-236.
doi:10.1590/S0100-879X2006000200009 .

Vučić, Vesna, Isenović, Esma R., Adžić, Miroslav, Ruždijić, Sabera, Radojčić, Marija, "Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells" in Brazilian Journal of Medical and Biological Research, 39, no. 2 (2006):227-236,
https://doi.org/10.1590/S0100-879X2006000200009 . .

TY  - CONF
AU  - Prokopović, J.
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Vučić, Vesna
AU  - Zarić, Božidarka
AU  - Radojčić, Marija B.
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9433
AB  - The molecular mechanism of gamma-ionizing radiation (IR) resistance of human prostate cancer cells PC-3 is not known. Since low-LET-IR effects are primarily achieved through generation of reactive oxygen species (ROS), IR-induced expression of ROSmetabolizing antioxidant enzymes, Mn- and CuZn-superoxide dismutase (Mn- and CuZnSOD) and catalase (CAT), and their upstream regulator transcription factor NFκB was followed. Significant elevation of both SODs was found in cells irradiated with 10- and 20 Gy, while CAT and NFkB expression was unchanged. Since, such conditions lead to accumulation of H2O2, it is concluded that radioresistance of PC-3 cells may emerge from positive feed-forward vicious circle established between H2O2 activation of NFκB and elevated MnSOD activity.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
T1  - Superoxide dismutases in radioresistant PC-3 human prostate carcinoma cells
SP  - 422
EP  - 424
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9433
ER  - 

@conference{
author = "Prokopović, J. and Adžić, Miroslav and Nićiforović, Ana and Vučić, Vesna and Zarić, Božidarka and Radojčić, Marija B.",
year = "2006",
abstract = "The molecular mechanism of gamma-ionizing radiation (IR) resistance of human prostate cancer cells PC-3 is not known. Since low-LET-IR effects are primarily achieved through generation of reactive oxygen species (ROS), IR-induced expression of ROSmetabolizing antioxidant enzymes, Mn- and CuZn-superoxide dismutase (Mn- and CuZnSOD) and catalase (CAT), and their upstream regulator transcription factor NFκB was followed. Significant elevation of both SODs was found in cells irradiated with 10- and 20 Gy, while CAT and NFkB expression was unchanged. Since, such conditions lead to accumulation of H2O2, it is concluded that radioresistance of PC-3 cells may emerge from positive feed-forward vicious circle established between H2O2 activation of NFκB and elevated MnSOD activity.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry",
title = "Superoxide dismutases in radioresistant PC-3 human prostate carcinoma cells",
pages = "422-424",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9433"
}

Prokopović, J., Adžić, M., Nićiforović, A., Vučić, V., Zarić, B.,& Radojčić, M. B.. (2006). Superoxide dismutases in radioresistant PC-3 human prostate carcinoma cells. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 422-424.
https://hdl.handle.net/21.15107/rcub_vinar_9433

Prokopović J, Adžić M, Nićiforović A, Vučić V, Zarić B, Radojčić MB. Superoxide dismutases in radioresistant PC-3 human prostate carcinoma cells. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry. 2006;:422-424.
https://hdl.handle.net/21.15107/rcub_vinar_9433 .

Prokopović, J., Adžić, Miroslav, Nićiforović, Ana, Vučić, Vesna, Zarić, Božidarka, Radojčić, Marija B., "Superoxide dismutases in radioresistant PC-3 human prostate carcinoma cells" in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry (2006):422-424,
https://hdl.handle.net/21.15107/rcub_vinar_9433 .

TY  - CONF
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Zarić, Božidarka
AU  - Vučić, Vesna
AU  - Radojčić, Marija B.
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9422
AB  - Naturally occurring phytoanthracycline, aloin, was used to radiosensitize HeLaS3 human cervix carcinoma cells. The results indicated that the cytotoxic adjuvant effect of aloin was synergistic with IR at all drug concentrations and comparable to the cytotoxicity of 5-10Gy IR alone. Radiosensitization of HeLaS3 cells was achieved by 60µM aloin which reduced IC50 dose of IR from 3.4- to 2Gy. The cell damage by both agents compromised cell capacity to conduct programmed cell death by apoptosis, and led to the synergic cytotoxic cell death by necrosis.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
T1  - Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells
VL  - 1
SP  - 344
EP  - 346
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9422
ER  - 

@conference{
author = "Nićiforović, Ana and Adžić, Miroslav and Zarić, Božidarka and Vučić, Vesna and Radojčić, Marija B.",
year = "2006",
abstract = "Naturally occurring phytoanthracycline, aloin, was used to radiosensitize HeLaS3 human cervix carcinoma cells. The results indicated that the cytotoxic adjuvant effect of aloin was synergistic with IR at all drug concentrations and comparable to the cytotoxicity of 5-10Gy IR alone. Radiosensitization of HeLaS3 cells was achieved by 60µM aloin which reduced IC50 dose of IR from 3.4- to 2Gy. The cell damage by both agents compromised cell capacity to conduct programmed cell death by apoptosis, and led to the synergic cytotoxic cell death by necrosis.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry",
title = "Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells",
volume = "1",
pages = "344-346",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9422"
}

Nićiforović, A., Adžić, M., Zarić, B., Vučić, V.,& Radojčić, M. B.. (2006). Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 344-346.
https://hdl.handle.net/21.15107/rcub_vinar_9422

Nićiforović A, Adžić M, Zarić B, Vučić V, Radojčić MB. Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry. 2006;1:344-346.
https://hdl.handle.net/21.15107/rcub_vinar_9422 .

Nićiforović, Ana, Adžić, Miroslav, Zarić, Božidarka, Vučić, Vesna, Radojčić, Marija B., "Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells" in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry, 1 (2006):344-346,
https://hdl.handle.net/21.15107/rcub_vinar_9422 .

TY  - CONF
AU  - Vučić, Vesna
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3203
C3  - European Journal of Cancer Supplements / EJC Supplements
T1  - Synergistic interaction between ionizing radiation and 8-chloro-adenosine 3 ,5 -monophosphate in PC-3 human prostate carcinoma cells
VL  - 3
IS  - 2
SP  - 258
EP  - 259
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3203
ER  - 

@conference{
author = "Vučić, Vesna and Radojčić, Marija and Ruždijić, Sabera",
year = "2005",
journal = "European Journal of Cancer Supplements / EJC Supplements",
title = "Synergistic interaction between ionizing radiation and 8-chloro-adenosine 3 ,5 -monophosphate in PC-3 human prostate carcinoma cells",
volume = "3",
number = "2",
pages = "258-259",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3203"
}

Vučić, V., Radojčić, M.,& Ruždijić, S.. (2005). Synergistic interaction between ionizing radiation and 8-chloro-adenosine 3 ,5 -monophosphate in PC-3 human prostate carcinoma cells. in European Journal of Cancer Supplements / EJC Supplements, 3(2), 258-259.
https://hdl.handle.net/21.15107/rcub_vinar_3203

Vučić V, Radojčić M, Ruždijić S. Synergistic interaction between ionizing radiation and 8-chloro-adenosine 3 ,5 -monophosphate in PC-3 human prostate carcinoma cells. in European Journal of Cancer Supplements / EJC Supplements. 2005;3(2):258-259.
https://hdl.handle.net/21.15107/rcub_vinar_3203 .

Vučić, Vesna, Radojčić, Marija, Ruždijić, Sabera, "Synergistic interaction between ionizing radiation and 8-chloro-adenosine 3 ,5 -monophosphate in PC-3 human prostate carcinoma cells" in European Journal of Cancer Supplements / EJC Supplements, 3, no. 2 (2005):258-259,
https://hdl.handle.net/21.15107/rcub_vinar_3203 .

TY  - CONF
AU  - Vučić, Vesna
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6488
AB  - Although radiotherapy and hormone therapy have been well established through a number of andomized studies, little is known about a possible interaction of both treatment modalities if they are given imultaneously. Radiotherapy and hormone therapy is combined in case of some types of breast cancer therapy. Estrogen signal is mediated within cells through specific binding to the estrogen receptor (ER).
The estrogen - ER protein complex binds with an increased affinity to a specific DNA sequence, called hormone response element (HRE), and consequently the transcription is initiated. ER expression appears to be generally low in normal breast epithelium, but it is over expressed in 60 - 70% of invasive
breast cancers. Cellular radiosensitivity and radiation induced DNA damage (double strand breaks) in both hormone sensitive and non-sensitive human breast cancer cell lines in presence or absence of estradiol were studied [Villalobos et al., Int. J. Radiat. Biol. 70(2), 161-169, 1996]. The findings of this study indicated that (1) sensitivity to radiation and the
proportion of proliferating cells are probably related, and (2) differences in radiosensitivity reflect differences in radiationinduced DNA damage.
The experimental study using plasmid DNA has been
performed in order to check how presence of estradiol
modifies initial yields of DNA damages induced by radiation. Method of agarose gel electrophoresis has been used to determine yields of particular types of DNA damages induced by g radiation of 6°Co. One or two HRE DNA base sequences were inserted into pCDNA3 plasmid. DNA plasmids were then irradiated in presence of increasing concentrations of estradiol and estradioI-ER protein. The yields of single and double strand breaks (SSB and DSB) determined as a function of absorbed dose and estradiol concentration in neutral and alkaline conditions will be presented and discussed. The radiomodifying properties of estradiol within cells will be estimated and compared to its effect on cell radiosensitivity due to induced grown-delay in hormone-sensitive cell lines.
C3  - Radiotherapy and Oncology
T1  - Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP
VL  - 73
SP  - S363
EP  - S363
DO  - 10.1016/S0167-8140(04)82718-X
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6488
ER  - 

@conference{
author = "Vučić, Vesna and Radojčić, Marija and Ruždijić, Sabera",
year = "2004",
abstract = "Although radiotherapy and hormone therapy have been well established through a number of andomized studies, little is known about a possible interaction of both treatment modalities if they are given imultaneously. Radiotherapy and hormone therapy is combined in case of some types of breast cancer therapy. Estrogen signal is mediated within cells through specific binding to the estrogen receptor (ER).
The estrogen - ER protein complex binds with an increased affinity to a specific DNA sequence, called hormone response element (HRE), and consequently the transcription is initiated. ER expression appears to be generally low in normal breast epithelium, but it is over expressed in 60 - 70% of invasive
breast cancers. Cellular radiosensitivity and radiation induced DNA damage (double strand breaks) in both hormone sensitive and non-sensitive human breast cancer cell lines in presence or absence of estradiol were studied [Villalobos et al., Int. J. Radiat. Biol. 70(2), 161-169, 1996]. The findings of this study indicated that (1) sensitivity to radiation and the
proportion of proliferating cells are probably related, and (2) differences in radiosensitivity reflect differences in radiationinduced DNA damage.
The experimental study using plasmid DNA has been
performed in order to check how presence of estradiol
modifies initial yields of DNA damages induced by radiation. Method of agarose gel electrophoresis has been used to determine yields of particular types of DNA damages induced by g radiation of 6°Co. One or two HRE DNA base sequences were inserted into pCDNA3 plasmid. DNA plasmids were then irradiated in presence of increasing concentrations of estradiol and estradioI-ER protein. The yields of single and double strand breaks (SSB and DSB) determined as a function of absorbed dose and estradiol concentration in neutral and alkaline conditions will be presented and discussed. The radiomodifying properties of estradiol within cells will be estimated and compared to its effect on cell radiosensitivity due to induced grown-delay in hormone-sensitive cell lines.",
journal = "Radiotherapy and Oncology",
title = "Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP",
volume = "73",
pages = "S363-S363",
doi = "10.1016/S0167-8140(04)82718-X",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6488"
}

Vučić, V., Radojčić, M.,& Ruždijić, S.. (2004). Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP. in Radiotherapy and Oncology, 73, S363-S363.
https://doi.org/10.1016/S0167-8140(04)82718-X
https://hdl.handle.net/21.15107/rcub_vinar_6488

Vučić V, Radojčić M, Ruždijić S. Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP. in Radiotherapy and Oncology. 2004;73:S363-S363.
doi:10.1016/S0167-8140(04)82718-X
https://hdl.handle.net/21.15107/rcub_vinar_6488 .

Vučić, Vesna, Radojčić, Marija, Ruždijić, Sabera, "Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP" in Radiotherapy and Oncology, 73 (2004):S363-S363,
https://doi.org/10.1016/S0167-8140(04)82718-X .,
https://hdl.handle.net/21.15107/rcub_vinar_6488 .

TY  - CONF
AU  - Vučić, Vesna
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2727
C3  - Annals of Oncology
T1  - Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP
VL  - 15
SP  - 115
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2727
ER  - 

@conference{
author = "Vučić, Vesna and Radojčić, Marija and Ruždijić, Sabera",
year = "2004",
journal = "Annals of Oncology",
title = "Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP",
volume = "15",
pages = "115-115",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2727"
}

Vučić, V., Radojčić, M.,& Ruždijić, S.. (2004). Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP. in Annals of Oncology, 15, 115-115.
https://hdl.handle.net/21.15107/rcub_vinar_2727

Vučić V, Radojčić M, Ruždijić S. Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP. in Annals of Oncology. 2004;15:115-115.
https://hdl.handle.net/21.15107/rcub_vinar_2727 .

Vučić, Vesna, Radojčić, Marija, Ruždijić, Sabera, "Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP" in Annals of Oncology, 15 (2004):115-115,
https://hdl.handle.net/21.15107/rcub_vinar_2727 .

TY  - JOUR
AU  - Vučić, Vesna
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Tišma, Nevena
AU  - Ruždijić, Sabera
AU  - Radojčić, Marija B.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10583
AB  - Despite the significant advances in cancer chemotherapy, radiotherapy still remains a method of choice for treatment of metastatic human prostate cancer. This study presents quantitative analysis of 60Co gamma-radiation effects on cell growth and cell death of metastatic human prostate cancer PC-3 cell line, performed in time (24-72h) and dose (2-20 Gy) dependent manner. The irradiated PC-3 cells were mostly dying by necrosis at late time intervals (72h), while apoptotic cell death was negligible. The EC50 or 50% of cytotoxicity was not achieved within the radiation doses used (2-20 Gy), but significant cell growth inhibition with IC50 of 10.4 Gy was observed. It is concluded that the increase in the radiation dose may have an important cytostatic effect, but for the complete eradication of metastatic prostate cancer novel cytotoxic drugs and radiosensitizers should be introduced as adjuvant.
T2  - Jugoslovenska medicinska biohemija
T1  - Cell death in irradiated prostate cancer cells assessed by flow cytometry
VL  - 23
IS  - 4
SP  - 343
EP  - 350
DO  - 10.2298/JMH0404343V
ER  - 

@article{
author = "Vučić, Vesna and Adžić, Miroslav and Nićiforović, Ana and Tišma, Nevena and Ruždijić, Sabera and Radojčić, Marija B.",
year = "2004",
abstract = "Despite the significant advances in cancer chemotherapy, radiotherapy still remains a method of choice for treatment of metastatic human prostate cancer. This study presents quantitative analysis of 60Co gamma-radiation effects on cell growth and cell death of metastatic human prostate cancer PC-3 cell line, performed in time (24-72h) and dose (2-20 Gy) dependent manner. The irradiated PC-3 cells were mostly dying by necrosis at late time intervals (72h), while apoptotic cell death was negligible. The EC50 or 50% of cytotoxicity was not achieved within the radiation doses used (2-20 Gy), but significant cell growth inhibition with IC50 of 10.4 Gy was observed. It is concluded that the increase in the radiation dose may have an important cytostatic effect, but for the complete eradication of metastatic prostate cancer novel cytotoxic drugs and radiosensitizers should be introduced as adjuvant.",
journal = "Jugoslovenska medicinska biohemija",
title = "Cell death in irradiated prostate cancer cells assessed by flow cytometry",
volume = "23",
number = "4",
pages = "343-350",
doi = "10.2298/JMH0404343V"
}

Vučić, V., Adžić, M., Nićiforović, A., Tišma, N., Ruždijić, S.,& Radojčić, M. B.. (2004). Cell death in irradiated prostate cancer cells assessed by flow cytometry. in Jugoslovenska medicinska biohemija, 23(4), 343-350.
https://doi.org/10.2298/JMH0404343V

Vučić V, Adžić M, Nićiforović A, Tišma N, Ruždijić S, Radojčić MB. Cell death in irradiated prostate cancer cells assessed by flow cytometry. in Jugoslovenska medicinska biohemija. 2004;23(4):343-350.
doi:10.2298/JMH0404343V .

Vučić, Vesna, Adžić, Miroslav, Nićiforović, Ana, Tišma, Nevena, Ruždijić, Sabera, Radojčić, Marija B., "Cell death in irradiated prostate cancer cells assessed by flow cytometry" in Jugoslovenska medicinska biohemija, 23, no. 4 (2004):343-350,
https://doi.org/10.2298/JMH0404343V . .

TY  - CONF
AU  - Vučić, Vesna
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Tišma, Nevena
AU  - Janković, Dragana
AU  - Ruždijić, Sabera
AU  - Radojčić, Marija B.
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12038
AB  - Background: Prostate cancer is the first as an incidence and the second as a cause of the oncologic mortality in the male population. There is a broad range of possibilities in the prostate cancer therapy. However, there is also much controversy on the most appropriate treatment in the various stages of the disease. Advanced disease is mostly treated by radiation therapy, sometimes in combination with hormone or chemotherapy. Irradiation induces damage of cell biomolecules, which can lead to the arrest in cell division, or to apoptotic or necrotic cell death. The aim of this study was to determine the dose dependence of radiation-induced cell death in two human prostate cancer cell lines, and to define the form of death of these cells. Methods: Human prostate cancer cell lines PC-3 and DU-145 were irradiated with 2 - 30 Gy from 60 Co g-source, at the dose rate of 20 Gy/h. The effect of irradiation on cell viability, morphology and DNA structure were followed 24 - 72 hours after treatment. Cells were analyzed by trypan blue exclusion assay, flow cytometry and DNA gel electrophoresis. Simultaneous staining of cells with Annexin V-FITC and propidium iodide enabled distinction of early apoptosis from late apoptosis and/or necrosis. Results: The results of trypan blue staining indicated that radiation-induced cell death was both time and dose dependent process. According to flow-cytometry and DNA fragmentation assay, necrosis was the prevailing form of the radiation-induced cell death in both PC-3 and DU-145 cells. The apoptosis occurred in insignificant number of cells, probably due to the mutant p53 gene present in both cell lines. The cell necrosis was dose dependent and was most pronounced 72 hours post treatment. Conclusion The prevailing form of radiation-induced PC-3 and DU-145 cell death is necrosis. Both PC-3 and DU-145 are rather radioresistant cell lines, as the dose necessary to induce 50% decrease in viable cell number is about 10 Gy.
C3  - Archive of Oncology
T1  - Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells
VL  - 11
IS  - 3
SP  - 197
EP  - 197
DO  - 10.2298/AOO0303197V
ER  - 

@conference{
author = "Vučić, Vesna and Nićiforović, Ana and Adžić, Miroslav and Tišma, Nevena and Janković, Dragana and Ruždijić, Sabera and Radojčić, Marija B.",
year = "2003",
abstract = "Background: Prostate cancer is the first as an incidence and the second as a cause of the oncologic mortality in the male population. There is a broad range of possibilities in the prostate cancer therapy. However, there is also much controversy on the most appropriate treatment in the various stages of the disease. Advanced disease is mostly treated by radiation therapy, sometimes in combination with hormone or chemotherapy. Irradiation induces damage of cell biomolecules, which can lead to the arrest in cell division, or to apoptotic or necrotic cell death. The aim of this study was to determine the dose dependence of radiation-induced cell death in two human prostate cancer cell lines, and to define the form of death of these cells. Methods: Human prostate cancer cell lines PC-3 and DU-145 were irradiated with 2 - 30 Gy from 60 Co g-source, at the dose rate of 20 Gy/h. The effect of irradiation on cell viability, morphology and DNA structure were followed 24 - 72 hours after treatment. Cells were analyzed by trypan blue exclusion assay, flow cytometry and DNA gel electrophoresis. Simultaneous staining of cells with Annexin V-FITC and propidium iodide enabled distinction of early apoptosis from late apoptosis and/or necrosis. Results: The results of trypan blue staining indicated that radiation-induced cell death was both time and dose dependent process. According to flow-cytometry and DNA fragmentation assay, necrosis was the prevailing form of the radiation-induced cell death in both PC-3 and DU-145 cells. The apoptosis occurred in insignificant number of cells, probably due to the mutant p53 gene present in both cell lines. The cell necrosis was dose dependent and was most pronounced 72 hours post treatment. Conclusion The prevailing form of radiation-induced PC-3 and DU-145 cell death is necrosis. Both PC-3 and DU-145 are rather radioresistant cell lines, as the dose necessary to induce 50% decrease in viable cell number is about 10 Gy.",
journal = "Archive of Oncology",
title = "Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells",
volume = "11",
number = "3",
pages = "197-197",
doi = "10.2298/AOO0303197V"
}

Vučić, V., Nićiforović, A., Adžić, M., Tišma, N., Janković, D., Ruždijić, S.,& Radojčić, M. B.. (2003). Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells. in Archive of Oncology, 11(3), 197-197.
https://doi.org/10.2298/AOO0303197V

Vučić V, Nićiforović A, Adžić M, Tišma N, Janković D, Ruždijić S, Radojčić MB. Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells. in Archive of Oncology. 2003;11(3):197-197.
doi:10.2298/AOO0303197V .

Vučić, Vesna, Nićiforović, Ana, Adžić, Miroslav, Tišma, Nevena, Janković, Dragana, Ruždijić, Sabera, Radojčić, Marija B., "Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells" in Archive of Oncology, 11, no. 3 (2003):197-197,
https://doi.org/10.2298/AOO0303197V . .