TY  - JOUR
AU  - Kolaković, Ana
AU  - Bundalo, Maja
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13103
AB  - Background/Aim. The renin-angiotensin system (RAS) is linked to the development of atherosclerosis (As), including its initiation and progression. Besides the well-known angio-tensin-converting enzyme (ACE), two newer RAS family members are related to vascular remodeling - ACE2 as a homolog of ACE and collectrin [transmembrane protein 27 (TMEM27)] as a homolog of ACE2. Up to now, a limited number of studies have examined the expression of these RAS components in advanced carotid plaque (CP) tissue based on the sex of the patients and plaque phenotypes (PPs). There are two ultrasonographically defined PPs - the hypoechogenic plaque (HoP) and the hyperechogenic plaque (HerP) phenotype. The aim of the study was to investigate whether there was a correlation between the expression of RAS components in the CP and the sex and PPs of patients. Methods. We examined 74 patients with advanced CP who underwent carotid endarterectomy. The intraplaque expression of RAS components was determined with the real-time polymerase chain reaction, using the TaqMan® gene expression assays and Western blot. A two-way ANOVA followed by a post-hoc Tukey test was performed for the statistical analysis of results. Results. No interaction was recorded between the sex of the patients and PPs in influencing the relative expression of ACE and TMEM27 messenger RNA (mRNA) (p > 0.05). In 56.06% of plaque samples, no expression of ACE2 mRNA was detected. Among the plaques where ACE2 mRNA expression was detected, its expression level was higher in females with the HoP phenotype compared to females with the HerP phenotype (p < 0.001). In patients with the HoP phenotype, females had higher expression of ACE2 mRNA than males (p < 0.05). In the male study group, ACE protein levels were significantly lower in the HoP phenotype compared to the HerP phenotype (p < 0.001). Fe-males with the HoP phenotype had significantly higher ACE protein levels than males with the HoP phenotype (p < 0.0001). Conclusion. Our results revealed alterations in the expression levels of ACE and ACE2, at the mRNA and protein levels, in advanced carotid As. These alterations are impacted by sex and PP and may indicate a switch from the balanced RAS/ACE/ACE2 axis in the healthy blood vessel to the unbalanced axis in vascular remodeling due to As.
AB  - Renin-angiotenzin sistem (RAS) povezan je sa
razvojem ateroskleroze (As), uključujući njen nastanak i
progresiju. Pored dobro poznatog angiotenzinkonvertujućeg enzima (angiotensin-converting enzyme – ACE),
dva nova člana RAS familije povezana su sa
remodelovanjem zidova krvnih sudova – ACE2 kao
homolog ACE i kolektrin [transmembrane protein 27
(TMEM27)] kao homolog ACE2. Do sada je mali broj
studija ispitivao ekspresiju komponenti RAS sistema u tkivu
uznapredovalog karotidnog plaka (KP) u odnosu na pol
bolesnika i fenotip plaka (FP). Postoje dva tipa KP
definisana primenom ultrazvuka – fenotip hipoehogenog
plaka (HoP) i fenotip hiperehogenog plaka (HerP). Cilj rada
bio je da se ispita da li postoji korelacija između ekspresije
komponenti RAS u KP i pola i FP bolesnika. Metode.
Ispitano je 74 bolesnika sa uznapredovalim KP koji su bili
podvrgnuti operativnoj proceduri karotidne
endarterektomije. Ekspresija komponenti RAS sistema u
tkivu plaka utvrđena je lančanom reakcijom polimeraze u
realnom vremenu (real-time polymerase chain reaction)primenom eseja TaqMan® tehnologije i metode Western blota. Dvosmerna analiza varijanse i Tukey post-hoc test korišćen su za statističku obradu rezultata. Rezultati. Nije utvrđena
interakcija FP i pola bolesnika u uticaju na relativnu
ekspresiju informacione RNK (iRNK) za ACE i TMEM27
(p > 0,05). U 56,06% uzoraka plaka nije detektovana
ekspresija iRNK za ACE2. U plakovima u kojima je
detektovana ekspresija iRNK za ACE2, njen nivo bio je viši
kod žena sa HoP u poređenju sa ženama sa HerP (p <
0,001). U grupi bolesnika sa fenotipom HoP, žene su imale
viši nivo iRNK za ACE2 nego muškarci (p < 0,05). U grupi
muškaraca, nivoi ekspresije ACE proteina bili suznačajno
niži u fenotipu HoP u poređenju sa HerP (p < 0,001). Žene
sa fenotipom HoP imale su značajno viši nivo ACE
proteina u poređenju sa muškarcima sa HoP (p < 0,0001).
Zaključak. Naši rezultati pokazali su da postoje promene
nivoa ekspresije ACE i ACE2, na nivou proteina i iRNK u
uznapredovaloj karotidnoj As. Te promene zavise od pola i
FP i mogu ukazivati na to da balans ose RAS/ACE/ACE2
koji postoji u zdravom krvnom sudu postaje poremećen
tokom remodelovanja zida krvnog suda usled As.
T2  - Vojnosanitetski pregled
T1  - The expression of renin-angiotensin system components in human carotid plaque
T1  - Ekspresija komponenti renin-angiotenzin sistema u humanom karotidnom plaku
DO  - 10.2298/VSP221028014K
ER  - 

@article{
author = "Kolaković, Ana and Bundalo, Maja and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra and Živković, Maja",
year = "2024",
abstract = "Background/Aim. The renin-angiotensin system (RAS) is linked to the development of atherosclerosis (As), including its initiation and progression. Besides the well-known angio-tensin-converting enzyme (ACE), two newer RAS family members are related to vascular remodeling - ACE2 as a homolog of ACE and collectrin [transmembrane protein 27 (TMEM27)] as a homolog of ACE2. Up to now, a limited number of studies have examined the expression of these RAS components in advanced carotid plaque (CP) tissue based on the sex of the patients and plaque phenotypes (PPs). There are two ultrasonographically defined PPs - the hypoechogenic plaque (HoP) and the hyperechogenic plaque (HerP) phenotype. The aim of the study was to investigate whether there was a correlation between the expression of RAS components in the CP and the sex and PPs of patients. Methods. We examined 74 patients with advanced CP who underwent carotid endarterectomy. The intraplaque expression of RAS components was determined with the real-time polymerase chain reaction, using the TaqMan® gene expression assays and Western blot. A two-way ANOVA followed by a post-hoc Tukey test was performed for the statistical analysis of results. Results. No interaction was recorded between the sex of the patients and PPs in influencing the relative expression of ACE and TMEM27 messenger RNA (mRNA) (p > 0.05). In 56.06% of plaque samples, no expression of ACE2 mRNA was detected. Among the plaques where ACE2 mRNA expression was detected, its expression level was higher in females with the HoP phenotype compared to females with the HerP phenotype (p < 0.001). In patients with the HoP phenotype, females had higher expression of ACE2 mRNA than males (p < 0.05). In the male study group, ACE protein levels were significantly lower in the HoP phenotype compared to the HerP phenotype (p < 0.001). Fe-males with the HoP phenotype had significantly higher ACE protein levels than males with the HoP phenotype (p < 0.0001). Conclusion. Our results revealed alterations in the expression levels of ACE and ACE2, at the mRNA and protein levels, in advanced carotid As. These alterations are impacted by sex and PP and may indicate a switch from the balanced RAS/ACE/ACE2 axis in the healthy blood vessel to the unbalanced axis in vascular remodeling due to As., Renin-angiotenzin sistem (RAS) povezan je sa
razvojem ateroskleroze (As), uključujući njen nastanak i
progresiju. Pored dobro poznatog angiotenzinkonvertujućeg enzima (angiotensin-converting enzyme – ACE),
dva nova člana RAS familije povezana su sa
remodelovanjem zidova krvnih sudova – ACE2 kao
homolog ACE i kolektrin [transmembrane protein 27
(TMEM27)] kao homolog ACE2. Do sada je mali broj
studija ispitivao ekspresiju komponenti RAS sistema u tkivu
uznapredovalog karotidnog plaka (KP) u odnosu na pol
bolesnika i fenotip plaka (FP). Postoje dva tipa KP
definisana primenom ultrazvuka – fenotip hipoehogenog
plaka (HoP) i fenotip hiperehogenog plaka (HerP). Cilj rada
bio je da se ispita da li postoji korelacija između ekspresije
komponenti RAS u KP i pola i FP bolesnika. Metode.
Ispitano je 74 bolesnika sa uznapredovalim KP koji su bili
podvrgnuti operativnoj proceduri karotidne
endarterektomije. Ekspresija komponenti RAS sistema u
tkivu plaka utvrđena je lančanom reakcijom polimeraze u
realnom vremenu (real-time polymerase chain reaction)primenom eseja TaqMan® tehnologije i metode Western blota. Dvosmerna analiza varijanse i Tukey post-hoc test korišćen su za statističku obradu rezultata. Rezultati. Nije utvrđena
interakcija FP i pola bolesnika u uticaju na relativnu
ekspresiju informacione RNK (iRNK) za ACE i TMEM27
(p > 0,05). U 56,06% uzoraka plaka nije detektovana
ekspresija iRNK za ACE2. U plakovima u kojima je
detektovana ekspresija iRNK za ACE2, njen nivo bio je viši
kod žena sa HoP u poređenju sa ženama sa HerP (p <
0,001). U grupi bolesnika sa fenotipom HoP, žene su imale
viši nivo iRNK za ACE2 nego muškarci (p < 0,05). U grupi
muškaraca, nivoi ekspresije ACE proteina bili suznačajno
niži u fenotipu HoP u poređenju sa HerP (p < 0,001). Žene
sa fenotipom HoP imale su značajno viši nivo ACE
proteina u poređenju sa muškarcima sa HoP (p < 0,0001).
Zaključak. Naši rezultati pokazali su da postoje promene
nivoa ekspresije ACE i ACE2, na nivou proteina i iRNK u
uznapredovaloj karotidnoj As. Te promene zavise od pola i
FP i mogu ukazivati na to da balans ose RAS/ACE/ACE2
koji postoji u zdravom krvnom sudu postaje poremećen
tokom remodelovanja zida krvnog suda usled As.",
journal = "Vojnosanitetski pregled",
title = "The expression of renin-angiotensin system components in human carotid plaque, Ekspresija komponenti renin-angiotenzin sistema u humanom karotidnom plaku",
doi = "10.2298/VSP221028014K"
}

Kolaković, A., Bundalo, M., Đurić, T., Končar, I., Stanković, A.,& Živković, M.. (2024). The expression of renin-angiotensin system components in human carotid plaque. in Vojnosanitetski pregled.
https://doi.org/10.2298/VSP221028014K

Kolaković A, Bundalo M, Đurić T, Končar I, Stanković A, Živković M. The expression of renin-angiotensin system components in human carotid plaque. in Vojnosanitetski pregled. 2024;.
doi:10.2298/VSP221028014K .

Kolaković, Ana, Bundalo, Maja, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, Živković, Maja, "The expression of renin-angiotensin system components in human carotid plaque" in Vojnosanitetski pregled (2024),
https://doi.org/10.2298/VSP221028014K . .

TY  - JOUR
AU  - Đorđević, Ana D.
AU  - Živković, Maja
AU  - Končar, Igor
AU  - Stanković, Aleksandra
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10046
AB  - ObjectivesGalectin-3 affects a variety of biological processes. It is encoded by LGALS-3, located in unique haplotype block in Caucasians. Most of the studies regarding the gal-3 role in atherosclerosis are focused exclusively on protein/mRNA levels. Genetic analyses of LGALS-3 are scarce. We sought to thoroughly examine the genetic background of gal-3 and to analyze tag variants that cover more than 80% variability of the LGALS-3 containing hap-block in association with carotid plaque presence (CPP). According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T cover 82% (r2 > 0.8) of the genetic variance of this hap-block. Our aims were to investigate possible association of rs4040064, rs11628437 and rs7159490 haplotypes with CPP in patients with advanced carotid atherosclerosis (CA) and to analyze their possible effect on LGALS-3 mRNA expression in carotid plaques.Materials and methodsStudy group consisted of 468 patients and 296 controls. Rs4040064, rs11628437, rs7159490 and LGALS-3 mRNA expression were detected by TaqMan® technology.ResultsWe have found that haplotype TAC was associated with the cerebrovascular insult (CVI) occurrence (OR = 1.68, 95% CI = 1.09-2.58, p = 0.02), compared to the referent haplotype. OR was adjusted for hypertension, age and BMI. TAC also showed higher, but not statistically significant, LGALS-3 expression in carotid plaques.ConclusionsOur results suggest that rs4040064, rs11628437 and rs7159490 bear no association with CPP, neither they affect LGALS-3 mRNA in carotid plaques. However, we showed a significant association of haplotype TAC with the CVI occurrence in CA patients from Serbia. Replication and validation of our results are required.
T2  - Journal of Stroke and Cerebrovascular Diseases
T2  - Journal of Stroke and Cerebrovascular DiseasesJournal of Stroke and Cerebrovascular Diseases
T1  - Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis
VL  - 31
IS  - 1
SP  - 106212
DO  - 10.1016/j.jstrokecerebrovasdis.2021.106212
ER  - 

@article{
author = "Đorđević, Ana D. and Živković, Maja and Končar, Igor and Stanković, Aleksandra and Kuveljić, Jovana and Đurić, Tamara",
year = "2022",
abstract = "ObjectivesGalectin-3 affects a variety of biological processes. It is encoded by LGALS-3, located in unique haplotype block in Caucasians. Most of the studies regarding the gal-3 role in atherosclerosis are focused exclusively on protein/mRNA levels. Genetic analyses of LGALS-3 are scarce. We sought to thoroughly examine the genetic background of gal-3 and to analyze tag variants that cover more than 80% variability of the LGALS-3 containing hap-block in association with carotid plaque presence (CPP). According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T cover 82% (r2 > 0.8) of the genetic variance of this hap-block. Our aims were to investigate possible association of rs4040064, rs11628437 and rs7159490 haplotypes with CPP in patients with advanced carotid atherosclerosis (CA) and to analyze their possible effect on LGALS-3 mRNA expression in carotid plaques.Materials and methodsStudy group consisted of 468 patients and 296 controls. Rs4040064, rs11628437, rs7159490 and LGALS-3 mRNA expression were detected by TaqMan® technology.ResultsWe have found that haplotype TAC was associated with the cerebrovascular insult (CVI) occurrence (OR = 1.68, 95% CI = 1.09-2.58, p = 0.02), compared to the referent haplotype. OR was adjusted for hypertension, age and BMI. TAC also showed higher, but not statistically significant, LGALS-3 expression in carotid plaques.ConclusionsOur results suggest that rs4040064, rs11628437 and rs7159490 bear no association with CPP, neither they affect LGALS-3 mRNA in carotid plaques. However, we showed a significant association of haplotype TAC with the CVI occurrence in CA patients from Serbia. Replication and validation of our results are required.",
journal = "Journal of Stroke and Cerebrovascular Diseases, Journal of Stroke and Cerebrovascular DiseasesJournal of Stroke and Cerebrovascular Diseases",
title = "Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis",
volume = "31",
number = "1",
pages = "106212",
doi = "10.1016/j.jstrokecerebrovasdis.2021.106212"
}

Đorđević, A. D., Živković, M., Končar, I., Stanković, A., Kuveljić, J.,& Đurić, T.. (2022). Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases, 31(1), 106212.
https://doi.org/10.1016/j.jstrokecerebrovasdis.2021.106212

Đorđević AD, Živković M, Končar I, Stanković A, Kuveljić J, Đurić T. Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases. 2022;31(1):106212.
doi:10.1016/j.jstrokecerebrovasdis.2021.106212 .

Đorđević, Ana D., Živković, Maja, Končar, Igor, Stanković, Aleksandra, Kuveljić, Jovana, Đurić, Tamara, "Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis" in Journal of Stroke and Cerebrovascular Diseases, 31, no. 1 (2022):106212,
https://doi.org/10.1016/j.jstrokecerebrovasdis.2021.106212 . .

TY  - JOUR
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Končar, Igor
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8348
AB  - Background: Carotid plaque is a hallmark of advanced carotid atherosclerosis and there is evidence of phosphatase and actin regulator 1 (PHACTR1) involvement in the processes that lead to atherosclerosis. PHACTR1 intronic variants have been associated with coronary artery disease and carotid dissection. Up to date the PHACTR1 haplotypes were not investigated in association with carotid plaque presence (CPP). So, the aims of this study were to investigate possible association of PHACTR1 haplotypes inferred from the intronic variants rs9349379, rs2026458 and rs2876300 with CPP in patients with advanced carotid atherosclerosis and to analyze their possible effect on PHACTR1 relative mRNA expression in carotid plaque tissue specimens. Methods: The study group consisted of 501 patients with evidence of carotid plaque presence admitted for carotid endarterectomy and 310 healthy controls. PHACTR1 rs9349379, rs2026458, rs2876300 and relative mRNA expression were detected by TaqMan® technology. Results: We have found significant and independent association of haplotype ACA with the CPP, compared to the referent haplotype GTA (adjusted OR = 1.54 95% CI = 1.07–2.21, p = 0.02). The OR was adjusted for gender, age, BMI, hypertension and total cholesterol. The relative expression of PHACTR1 mRNA in carotid plaque tissue proved to be significantly higher in carriers of the ACG haplotype compared to the referent haplotype GTA (p = 0.03). Conclusion: Our results suggest that PHACTR1 haplotypes inferred from the variants rs9349379, rs2026458 and rs2876300 affect PHACTR1 mRNA and bear the risk for CPP in patients with advanced carotid atherosclerosis. Further replication and validation studies are inevitable. © 2019 Elsevier B.V.
T2  - Gene
T1  - PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue
VL  - 710
SP  - 273
EP  - 278
DO  - 10.1016/j.gene.2019.06.020
ER  - 

@article{
author = "Kuveljić, Jovana and Đurić, Tamara and Stanković, Aleksandra and Končar, Igor and Alavantić, Dragan and Živković, Maja",
year = "2019",
abstract = "Background: Carotid plaque is a hallmark of advanced carotid atherosclerosis and there is evidence of phosphatase and actin regulator 1 (PHACTR1) involvement in the processes that lead to atherosclerosis. PHACTR1 intronic variants have been associated with coronary artery disease and carotid dissection. Up to date the PHACTR1 haplotypes were not investigated in association with carotid plaque presence (CPP). So, the aims of this study were to investigate possible association of PHACTR1 haplotypes inferred from the intronic variants rs9349379, rs2026458 and rs2876300 with CPP in patients with advanced carotid atherosclerosis and to analyze their possible effect on PHACTR1 relative mRNA expression in carotid plaque tissue specimens. Methods: The study group consisted of 501 patients with evidence of carotid plaque presence admitted for carotid endarterectomy and 310 healthy controls. PHACTR1 rs9349379, rs2026458, rs2876300 and relative mRNA expression were detected by TaqMan® technology. Results: We have found significant and independent association of haplotype ACA with the CPP, compared to the referent haplotype GTA (adjusted OR = 1.54 95% CI = 1.07–2.21, p = 0.02). The OR was adjusted for gender, age, BMI, hypertension and total cholesterol. The relative expression of PHACTR1 mRNA in carotid plaque tissue proved to be significantly higher in carriers of the ACG haplotype compared to the referent haplotype GTA (p = 0.03). Conclusion: Our results suggest that PHACTR1 haplotypes inferred from the variants rs9349379, rs2026458 and rs2876300 affect PHACTR1 mRNA and bear the risk for CPP in patients with advanced carotid atherosclerosis. Further replication and validation studies are inevitable. © 2019 Elsevier B.V.",
journal = "Gene",
title = "PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue",
volume = "710",
pages = "273-278",
doi = "10.1016/j.gene.2019.06.020"
}

Kuveljić, J., Đurić, T., Stanković, A., Končar, I., Alavantić, D.,& Živković, M.. (2019). PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue. in Gene, 710, 273-278.
https://doi.org/10.1016/j.gene.2019.06.020

Kuveljić J, Đurić T, Stanković A, Končar I, Alavantić D, Živković M. PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue. in Gene. 2019;710:273-278.
doi:10.1016/j.gene.2019.06.020 .

Kuveljić, Jovana, Đurić, Tamara, Stanković, Aleksandra, Končar, Igor, Alavantić, Dragan, Živković, Maja, "PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue" in Gene, 710 (2019):273-278,
https://doi.org/10.1016/j.gene.2019.06.020 . .

TY  - CONF
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Stanković, Aleksandra
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S002191501830707X
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7952
C3  - Atherosclerosis
T1  - The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype
VL  - 275
SP  - e135
DO  - 10.1016/j.atherosclerosis.2018.06.395
ER  - 

@conference{
author = "Kolaković, Ana and Živković, Maja and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra",
year = "2018",
journal = "Atherosclerosis",
title = "The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype",
volume = "275",
pages = "e135",
doi = "10.1016/j.atherosclerosis.2018.06.395"
}

Kolaković, A., Živković, M., Đurić, T., Končar, I.,& Stanković, A.. (2018). The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype. in Atherosclerosis, 275, e135.
https://doi.org/10.1016/j.atherosclerosis.2018.06.395

Kolaković A, Živković M, Đurić T, Končar I, Stanković A. The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype. in Atherosclerosis. 2018;275:e135.
doi:10.1016/j.atherosclerosis.2018.06.395 .

Kolaković, Ana, Živković, Maja, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, "The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype" in Atherosclerosis, 275 (2018):e135,
https://doi.org/10.1016/j.atherosclerosis.2018.06.395 . .

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Ivancevic, Ilija
AU  - Končar, Igor
AU  - Milašinović, Dejan
AU  - Stanković, Goran
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1843
AB  - The 9p21.3 region is rich in regulatory elements and the variants in this region had been robustly associated with carotid plaque (CP) and coronary artery disease (CAD). Recently, the HACD4 was detected as one of the six 9p21.3 differentially expressed genes associated with accelerated atherosclerosis and greater mean lesion area in the Athsq1 congenic mice. We aimed to investigate association of two potentially regulatory HACD4 variants (rs36212560 I/D, rs2275888 T/C) and their haplotypes with CP occurrence and the level of HACD4 and FOCAD mRNA in human CP tissue. Association study was replicated in CAD patients who suffered the first myocardial infarction. Study included 477 CP patients, 303 healthy controls and replication sample of 224 CAD males from the population of Serbia. Genotypes were determined by polymerase chain reaction (PCR) and real-time PCR using TaqMan (R) technology. The gene expression was detected with TaqMan (R) technology. We have found significant and independent association of DT haplotype with CP presence in men (adjusted OR = 1.64 CI = 1.12-2.42, p = 0.011). The result was replicated in CAD males (adjusted OR = 1.84 CI = 1.21-2.80, p = 0.004). We have found significant effect of the HACD4 rs2275888 on FOCAD mRNA level in human CP tissue. Correction for multiple testing was performed. Independent association of HACD4 haplotypes with atherosclerotic phenotypes connotes a further validation and replication in larger cohorts as well as functional studies to enlighten the potential mechanism of its action in pathophysiology of atherosclerosis.
T2  - Gene
T1  - The HACD4 haplotype as a risk factor for atherosclerosis in males
VL  - 641
SP  - 35
EP  - 40
DO  - 10.1016/j.gene.2017.10.030
ER  - 

@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Ivancevic, Ilija and Končar, Igor and Milašinović, Dejan and Stanković, Goran and Alavantić, Dragan and Živković, Maja",
year = "2018",
abstract = "The 9p21.3 region is rich in regulatory elements and the variants in this region had been robustly associated with carotid plaque (CP) and coronary artery disease (CAD). Recently, the HACD4 was detected as one of the six 9p21.3 differentially expressed genes associated with accelerated atherosclerosis and greater mean lesion area in the Athsq1 congenic mice. We aimed to investigate association of two potentially regulatory HACD4 variants (rs36212560 I/D, rs2275888 T/C) and their haplotypes with CP occurrence and the level of HACD4 and FOCAD mRNA in human CP tissue. Association study was replicated in CAD patients who suffered the first myocardial infarction. Study included 477 CP patients, 303 healthy controls and replication sample of 224 CAD males from the population of Serbia. Genotypes were determined by polymerase chain reaction (PCR) and real-time PCR using TaqMan (R) technology. The gene expression was detected with TaqMan (R) technology. We have found significant and independent association of DT haplotype with CP presence in men (adjusted OR = 1.64 CI = 1.12-2.42, p = 0.011). The result was replicated in CAD males (adjusted OR = 1.84 CI = 1.21-2.80, p = 0.004). We have found significant effect of the HACD4 rs2275888 on FOCAD mRNA level in human CP tissue. Correction for multiple testing was performed. Independent association of HACD4 haplotypes with atherosclerotic phenotypes connotes a further validation and replication in larger cohorts as well as functional studies to enlighten the potential mechanism of its action in pathophysiology of atherosclerosis.",
journal = "Gene",
title = "The HACD4 haplotype as a risk factor for atherosclerosis in males",
volume = "641",
pages = "35-40",
doi = "10.1016/j.gene.2017.10.030"
}

Životić, I., Đurić, T., Stanković, A., Ivancevic, I., Končar, I., Milašinović, D., Stanković, G., Alavantić, D.,& Živković, M.. (2018). The HACD4 haplotype as a risk factor for atherosclerosis in males. in Gene, 641, 35-40.
https://doi.org/10.1016/j.gene.2017.10.030

Životić I, Đurić T, Stanković A, Ivancevic I, Končar I, Milašinović D, Stanković G, Alavantić D, Živković M. The HACD4 haplotype as a risk factor for atherosclerosis in males. in Gene. 2018;641:35-40.
doi:10.1016/j.gene.2017.10.030 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Ivancevic, Ilija, Končar, Igor, Milašinović, Dejan, Stanković, Goran, Alavantić, Dragan, Živković, Maja, "The HACD4 haplotype as a risk factor for atherosclerosis in males" in Gene, 641 (2018):35-40,
https://doi.org/10.1016/j.gene.2017.10.030 . .

TY  - JOUR
AU  - Stanković, Aleksandra
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Bundalo, Maja M.
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1045
AB  - Background and Aims: The principal biologic effects of the renin-angiotensin system are mediated by activation of the AT1R receptor. The microRNA miR-155 regulates AT1R expression, with both its, and AT1Rs activity, linked to atherosclerosis. Target sites for miR-155 lie within the 3 UTR of the human AT1R gene, and include the AT1R A1166C polymorphism. Thus far, only levels of circulating miR-155 have been investigated with respect to A1166C genotypes. We hypothesized that the A1166C polymorphism could correlate with different, ultra-sonographically defined plaque phenotypes, as well as with an altered expression of AT1R mRNA and protein in human carotid plaques (CP), and altered expression of miR-155 in patients with advanced atherosclerosis. Methods: Our study cohort comprised 411 patients with advanced carotid atherosclerosis (298 hyperechoic; 113 hypoechoic plaques). PCR analyses identified A1166C genotypes; quantitative real-time PCR determined AT1R and miR-155 expression levels, with AT1R protein expression evaluated by western blot. Results: Genotypes containing the C allele bore a significant association with the hypoechoic plaque phenotype (adjusted OR 1.87, 95% CI 1.16-3.00, p = 0.01). The expression of AT1R mRNA and miR-155 were significantly up-regulated in the CPs of CC genotype carriers compared to the AA/AC genotypes (p = 0.032, p = 0.015, respectively). AT1R protein expression was also significantly higher for CC genotypes (p LT 0.01). Conclusion: Our results indicate that the AT1R A1166C polymorphism impacts an ultrasonographicallydefined human plaque phenotype, with intra-plaque AT1R and miR-155 expression altered in advanced carotid atherosclerosis. Validation and replication of these data should contribute to an improved personalized therapy with which to prevent carotid atherosclerosis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Atherosclerosis
T1  - Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques
VL  - 248
SP  - 132
EP  - 139
DO  - 10.1016/j.atherosclerosis.2016.02.032
ER  - 

@article{
author = "Stanković, Aleksandra and Kolaković, Ana and Živković, Maja and Đurić, Tamara and Bundalo, Maja M. and Končar, Igor and Davidović, Lazar and Alavantić, Dragan",
year = "2016",
abstract = "Background and Aims: The principal biologic effects of the renin-angiotensin system are mediated by activation of the AT1R receptor. The microRNA miR-155 regulates AT1R expression, with both its, and AT1Rs activity, linked to atherosclerosis. Target sites for miR-155 lie within the 3 UTR of the human AT1R gene, and include the AT1R A1166C polymorphism. Thus far, only levels of circulating miR-155 have been investigated with respect to A1166C genotypes. We hypothesized that the A1166C polymorphism could correlate with different, ultra-sonographically defined plaque phenotypes, as well as with an altered expression of AT1R mRNA and protein in human carotid plaques (CP), and altered expression of miR-155 in patients with advanced atherosclerosis. Methods: Our study cohort comprised 411 patients with advanced carotid atherosclerosis (298 hyperechoic; 113 hypoechoic plaques). PCR analyses identified A1166C genotypes; quantitative real-time PCR determined AT1R and miR-155 expression levels, with AT1R protein expression evaluated by western blot. Results: Genotypes containing the C allele bore a significant association with the hypoechoic plaque phenotype (adjusted OR 1.87, 95% CI 1.16-3.00, p = 0.01). The expression of AT1R mRNA and miR-155 were significantly up-regulated in the CPs of CC genotype carriers compared to the AA/AC genotypes (p = 0.032, p = 0.015, respectively). AT1R protein expression was also significantly higher for CC genotypes (p LT 0.01). Conclusion: Our results indicate that the AT1R A1166C polymorphism impacts an ultrasonographicallydefined human plaque phenotype, with intra-plaque AT1R and miR-155 expression altered in advanced carotid atherosclerosis. Validation and replication of these data should contribute to an improved personalized therapy with which to prevent carotid atherosclerosis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Atherosclerosis",
title = "Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques",
volume = "248",
pages = "132-139",
doi = "10.1016/j.atherosclerosis.2016.02.032"
}

Stanković, A., Kolaković, A., Živković, M., Đurić, T., Bundalo, M. M., Končar, I., Davidović, L.,& Alavantić, D.. (2016). Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques. in Atherosclerosis
Elsevier., 248, 132-139.
https://doi.org/10.1016/j.atherosclerosis.2016.02.032

Stanković A, Kolaković A, Živković M, Đurić T, Bundalo MM, Končar I, Davidović L, Alavantić D. Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques. in Atherosclerosis. 2016;248:132-139.
doi:10.1016/j.atherosclerosis.2016.02.032 .

Stanković, Aleksandra, Kolaković, Ana, Živković, Maja, Đurić, Tamara, Bundalo, Maja M., Končar, Igor, Davidović, Lazar, Alavantić, Dragan, "Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques" in Atherosclerosis, 248 (2016):132-139,
https://doi.org/10.1016/j.atherosclerosis.2016.02.032 . .

TY  - JOUR
AU  - Kolaković, Ana
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Radak, Đorđe J.
AU  - Alavantić, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1123
AB  - Background: The angiotensin II type 2 receptor (AT2R) - 1332 A/G polymorphism has been denoted as functional and associated with certain cardiovascular disease phenotypes. However, there are no studies considering the association of this gene polymorphism with carotid atherosclerosis (CA) and cerebrovascular events. Therefore, the aim of our study was to investigate a possible association of the AT2R - 1332 A/G polymorphism with the occurrence of carotid plaques (CPs) and history of cerebrovascular insult (CVI) in advanced CA. Methods: The study group included 381 controls and 509 patients with CA consecutively admitted for endarterectomy. Genotyping was determined by polymerase chain reaction-restriction fragment length polymorphism method. The association was analyzed separately for males and females because the AT2R gene is located on the X chromosome. Results: The AT2R - 1332 GG genotype was associated with the advanced CA in the female study group (recessive model of inheritance, AA+AG versus GG; adjusted odds ratio [OR] = 2.25; 95% confidence interval [CI] 1.17-4.33; P=.01). In the male subgroup of patients with CA, the significant overrepresentation of G/- hemizygote was detected in patients with CVI compared to male patients without this event (crude OR = 2.05, 95% CI 1.20-3.50, P=.008). Conclusions: This study suggests a gender-specific association between the AT2R -1332 A/G polymorphism and the occurrence of CP and the history of CVI in advanced CA, but further replication studies are needed. (C) 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.
T2  - Journal of Stroke and Cerebrovascular Diseases
T1  - Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis
VL  - 25
IS  - 7
SP  - 1622
EP  - 1630
DO  - 10.1016/j.jstrokecerebrovasdis.2016.03.011
ER  - 

@article{
author = "Kolaković, Ana and Stanković, Aleksandra and Đurić, Tamara and Živković, Maja and Končar, Igor and Davidović, Lazar and Radak, Đorđe J. and Alavantić, Dragan",
year = "2016",
abstract = "Background: The angiotensin II type 2 receptor (AT2R) - 1332 A/G polymorphism has been denoted as functional and associated with certain cardiovascular disease phenotypes. However, there are no studies considering the association of this gene polymorphism with carotid atherosclerosis (CA) and cerebrovascular events. Therefore, the aim of our study was to investigate a possible association of the AT2R - 1332 A/G polymorphism with the occurrence of carotid plaques (CPs) and history of cerebrovascular insult (CVI) in advanced CA. Methods: The study group included 381 controls and 509 patients with CA consecutively admitted for endarterectomy. Genotyping was determined by polymerase chain reaction-restriction fragment length polymorphism method. The association was analyzed separately for males and females because the AT2R gene is located on the X chromosome. Results: The AT2R - 1332 GG genotype was associated with the advanced CA in the female study group (recessive model of inheritance, AA+AG versus GG; adjusted odds ratio [OR] = 2.25; 95% confidence interval [CI] 1.17-4.33; P=.01). In the male subgroup of patients with CA, the significant overrepresentation of G/- hemizygote was detected in patients with CVI compared to male patients without this event (crude OR = 2.05, 95% CI 1.20-3.50, P=.008). Conclusions: This study suggests a gender-specific association between the AT2R -1332 A/G polymorphism and the occurrence of CP and the history of CVI in advanced CA, but further replication studies are needed. (C) 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.",
journal = "Journal of Stroke and Cerebrovascular Diseases",
title = "Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis",
volume = "25",
number = "7",
pages = "1622-1630",
doi = "10.1016/j.jstrokecerebrovasdis.2016.03.011"
}

Kolaković, A., Stanković, A., Đurić, T., Živković, M., Končar, I., Davidović, L., Radak, Đ. J.,& Alavantić, D.. (2016). Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases, 25(7), 1622-1630.
https://doi.org/10.1016/j.jstrokecerebrovasdis.2016.03.011

Kolaković A, Stanković A, Đurić T, Živković M, Končar I, Davidović L, Radak ĐJ, Alavantić D. Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases. 2016;25(7):1622-1630.
doi:10.1016/j.jstrokecerebrovasdis.2016.03.011 .

Kolaković, Ana, Stanković, Aleksandra, Đurić, Tamara, Živković, Maja, Končar, Igor, Davidović, Lazar, Radak, Đorđe J., Alavantić, Dragan, "Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis" in Journal of Stroke and Cerebrovascular Diseases, 25, no. 7 (2016):1622-1630,
https://doi.org/10.1016/j.jstrokecerebrovasdis.2016.03.011 . .

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Đorđević, Ana
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1126
AB  - Single nucleotide polymorphisms from the chromosome locus 9p21 are reported to carry a risk for various cardiovascular diseases. One of the lead single nucleotide polymorphisms, rs10757278, was mostly investigated in association with coronary artery disease but rarely with carotid atherosclerosis. In this study, we aimed to analyze the association of rs10757278 A/G polymorphism with carotid plaque presence in advanced carotid atherosclerosis. The study included 803 participants, 486 patients with high-grade stenosis ( GT 70%) who were undergoing carotid endarterectomy and 317 controls from Serbian population. Genotypes were determined using the real-time polymerase chain reaction. According to the recessive model of inheritance, GG genotype was significantly and independently associated with carotid plaque in females only (odds ratio 2.42, CI = 1.20-4.90, P = 0.013). Odds ratio was adjusted for age, body mass index, hypertension, TC, LDLC, HDLC and TG, and P value was corrected for multiple comparisons. Our preliminary findings suggest a gender-specific association of rs10757278 polymorphism with carotid plaque. Further studies on larger sample and in genetically and environmentally similar populations are needed.
T2  - Experimental Biology and Medicine
T1  - 9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner
VL  - 241
IS  - 11
SP  - 1210
EP  - 1216
DO  - 10.1177/1535370216636718
ER  - 

@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Đorđević, Ana and Končar, Igor and Davidović, Lazar and Alavantić, Dragan and Živković, Maja",
year = "2016",
abstract = "Single nucleotide polymorphisms from the chromosome locus 9p21 are reported to carry a risk for various cardiovascular diseases. One of the lead single nucleotide polymorphisms, rs10757278, was mostly investigated in association with coronary artery disease but rarely with carotid atherosclerosis. In this study, we aimed to analyze the association of rs10757278 A/G polymorphism with carotid plaque presence in advanced carotid atherosclerosis. The study included 803 participants, 486 patients with high-grade stenosis ( GT 70%) who were undergoing carotid endarterectomy and 317 controls from Serbian population. Genotypes were determined using the real-time polymerase chain reaction. According to the recessive model of inheritance, GG genotype was significantly and independently associated with carotid plaque in females only (odds ratio 2.42, CI = 1.20-4.90, P = 0.013). Odds ratio was adjusted for age, body mass index, hypertension, TC, LDLC, HDLC and TG, and P value was corrected for multiple comparisons. Our preliminary findings suggest a gender-specific association of rs10757278 polymorphism with carotid plaque. Further studies on larger sample and in genetically and environmentally similar populations are needed.",
journal = "Experimental Biology and Medicine",
title = "9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner",
volume = "241",
number = "11",
pages = "1210-1216",
doi = "10.1177/1535370216636718"
}

Životić, I., Đurić, T., Stanković, A., Đorđević, A., Končar, I., Davidović, L., Alavantić, D.,& Živković, M.. (2016). 9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner. in Experimental Biology and Medicine, 241(11), 1210-1216.
https://doi.org/10.1177/1535370216636718

Životić I, Đurić T, Stanković A, Đorđević A, Končar I, Davidović L, Alavantić D, Živković M. 9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner. in Experimental Biology and Medicine. 2016;241(11):1210-1216.
doi:10.1177/1535370216636718 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Đorđević, Ana, Končar, Igor, Davidović, Lazar, Alavantić, Dragan, Živković, Maja, "9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner" in Experimental Biology and Medicine, 241, no. 11 (2016):1210-1216,
https://doi.org/10.1177/1535370216636718 . .

TY  - JOUR
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
AU  - Đurić, Tamara
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1291
AB  - BackgroundPrevious research has shown that there is an association between galectin-3 (gal-3) protein and cardiovascular pathology. The aim of this study was to investigate the effects of rs2274273 and rs17128183 on genetic susceptibility to advanced carotid atherosclerosis (CA) and its complications. The rs2274273 has been singled out as the lead SNP of the haplotype block containing LGALS-3 (gal-3 gene) associated with gal-3 circulating levels, while rs17128183 constitutes a potentially functional SNP of the same hap-block. We further sought to determine whether these genetic variants have an impact on the expression of LGALS-3 mRNA in human carotid atherosclerotic plaque tissue. MethodsThe study encompassed 300 control subjects and 485 patients with advanced CA who had undergone carotid endarterectomy. Rs2274273, rs17128183, and LGALS-3 relative mRNA expression was detected by means of real-time PCR (TaqMan((R)) technology). ResultsThere were no statistically significant associations of the investigated genetic variants with susceptibility to advanced CA, nor did we find any associations in terms of ultrasonographically defined plaque phenotypes. The relative expression of LGALS-3 mRNA proved to be significantly higher in carriers of the rare alleles (P=0.039) for both genetic variants. ConclusionOur exploratory results suggest that while rs2274273 and rs17128183 bear no association with the risk of advanced CA or CA-related complications, these genetic variants are likely to affect LGALS-3 expression levels. In order to reach a definitive conclusion on the role played by rs2274273 and rs17128183 in advanced CA, our results should be further validated.
T2  - Journal of Clinical Laboratory Analysis
T1  - Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study
VL  - 30
IS  - 6
SP  - 1150
EP  - 1157
DO  - 10.1002/jcla.21996
ER  - 

@article{
author = "Đorđević, Ana and Živković, Maja and Stanković, Aleksandra and Životić, Ivan and Končar, Igor and Davidović, Lazar and Alavantić, Dragan and Đurić, Tamara",
year = "2016",
abstract = "BackgroundPrevious research has shown that there is an association between galectin-3 (gal-3) protein and cardiovascular pathology. The aim of this study was to investigate the effects of rs2274273 and rs17128183 on genetic susceptibility to advanced carotid atherosclerosis (CA) and its complications. The rs2274273 has been singled out as the lead SNP of the haplotype block containing LGALS-3 (gal-3 gene) associated with gal-3 circulating levels, while rs17128183 constitutes a potentially functional SNP of the same hap-block. We further sought to determine whether these genetic variants have an impact on the expression of LGALS-3 mRNA in human carotid atherosclerotic plaque tissue. MethodsThe study encompassed 300 control subjects and 485 patients with advanced CA who had undergone carotid endarterectomy. Rs2274273, rs17128183, and LGALS-3 relative mRNA expression was detected by means of real-time PCR (TaqMan((R)) technology). ResultsThere were no statistically significant associations of the investigated genetic variants with susceptibility to advanced CA, nor did we find any associations in terms of ultrasonographically defined plaque phenotypes. The relative expression of LGALS-3 mRNA proved to be significantly higher in carriers of the rare alleles (P=0.039) for both genetic variants. ConclusionOur exploratory results suggest that while rs2274273 and rs17128183 bear no association with the risk of advanced CA or CA-related complications, these genetic variants are likely to affect LGALS-3 expression levels. In order to reach a definitive conclusion on the role played by rs2274273 and rs17128183 in advanced CA, our results should be further validated.",
journal = "Journal of Clinical Laboratory Analysis",
title = "Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study",
volume = "30",
number = "6",
pages = "1150-1157",
doi = "10.1002/jcla.21996"
}

Đorđević, A., Živković, M., Stanković, A., Životić, I., Končar, I., Davidović, L., Alavantić, D.,& Đurić, T.. (2016). Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study. in Journal of Clinical Laboratory Analysis, 30(6), 1150-1157.
https://doi.org/10.1002/jcla.21996

Đorđević A, Živković M, Stanković A, Životić I, Končar I, Davidović L, Alavantić D, Đurić T. Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study. in Journal of Clinical Laboratory Analysis. 2016;30(6):1150-1157.
doi:10.1002/jcla.21996 .

Đorđević, Ana, Živković, Maja, Stanković, Aleksandra, Životić, Ivan, Končar, Igor, Davidović, Lazar, Alavantić, Dragan, Đurić, Tamara, "Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study" in Journal of Clinical Laboratory Analysis, 30, no. 6 (2016):1150-1157,
https://doi.org/10.1002/jcla.21996 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Stojković, Ljiljana S.
AU  - Jovanović, Ivan G.
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Veljković, Nevena V.
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/481
AB  - Aim: Chemokine CXC ligand 16 (CXCL16) has chemoattractive, adhesive and scavenging properties and may play a role in the formation of atherosclerotic lesions. However, studies of CXCL16 polymorphisms in patients with atherosclerosis are scarce. The missense polymorphisms I123T and A181V are potentially important factors in the regulation of presentation and shedding of the CXCL16 chemokine domain. The aim of this study was to analyze the association between I123T and A181V polymorphism haplotypes and the accumulation of carotid plaque as well as the effect of the haplotype on the CXCL16 mRNA expression in carotid plaques in patients with advanced atherosclerosis. Additionally, we performed a bioinformatic prediction analysis of the impact of CXCL16 protein sequence variation on CXCL16-CXCR6 interactions and analyzed the soluble CXCL16 plasma levels according to the CXCL16 haplotype. Methods: This study evaluated a total of 733 participants, including 283 controls and 450 patients with carotid atherosclerosis (CA) undergoing endarterectomy. Analyses of the polymorphisms and the gene expression were performed using real-time PCR. The soluble CXCL16 levels were measured with ELISA. Results: The missense allele haplotype, T123V181, was found to be significantly and independently associated with the occurrence of CA plaque (OR=1.27; 1.02-1.57, p=0.03). This haplotype was predicted to significantly change the CXCL16-CXCR6 interaction, compared to I123A181. Neither the CXCL16 mRNA expression in the human plaques nor the soluble CXCL16 plasma levels differed according to the haplotype. Conclusions: These results indicate that the CXCL16 T123V181 haplotype is a moderate genetic risk factor for the development of carotid plaque. Further functional and replication studies are needed to clarify the mechanisms by which this combination of alleles promotes advanced CA and validate its impact on disease progression.
T2  - Journal of Atherosclerosis and Thrombosis
T1  - CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results
VL  - 22
IS  - 1
SP  - 10
EP  - 20
DO  - 10.5551/jat.24299
ER  - 

@article{
author = "Živković, Maja and Đurić, Tamara and Stojković, Ljiljana S. and Jovanović, Ivan G. and Končar, Igor and Davidović, Lazar and Veljković, Nevena V. and Alavantić, Dragan and Stanković, Aleksandra",
year = "2015",
abstract = "Aim: Chemokine CXC ligand 16 (CXCL16) has chemoattractive, adhesive and scavenging properties and may play a role in the formation of atherosclerotic lesions. However, studies of CXCL16 polymorphisms in patients with atherosclerosis are scarce. The missense polymorphisms I123T and A181V are potentially important factors in the regulation of presentation and shedding of the CXCL16 chemokine domain. The aim of this study was to analyze the association between I123T and A181V polymorphism haplotypes and the accumulation of carotid plaque as well as the effect of the haplotype on the CXCL16 mRNA expression in carotid plaques in patients with advanced atherosclerosis. Additionally, we performed a bioinformatic prediction analysis of the impact of CXCL16 protein sequence variation on CXCL16-CXCR6 interactions and analyzed the soluble CXCL16 plasma levels according to the CXCL16 haplotype. Methods: This study evaluated a total of 733 participants, including 283 controls and 450 patients with carotid atherosclerosis (CA) undergoing endarterectomy. Analyses of the polymorphisms and the gene expression were performed using real-time PCR. The soluble CXCL16 levels were measured with ELISA. Results: The missense allele haplotype, T123V181, was found to be significantly and independently associated with the occurrence of CA plaque (OR=1.27; 1.02-1.57, p=0.03). This haplotype was predicted to significantly change the CXCL16-CXCR6 interaction, compared to I123A181. Neither the CXCL16 mRNA expression in the human plaques nor the soluble CXCL16 plasma levels differed according to the haplotype. Conclusions: These results indicate that the CXCL16 T123V181 haplotype is a moderate genetic risk factor for the development of carotid plaque. Further functional and replication studies are needed to clarify the mechanisms by which this combination of alleles promotes advanced CA and validate its impact on disease progression.",
journal = "Journal of Atherosclerosis and Thrombosis",
title = "CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results",
volume = "22",
number = "1",
pages = "10-20",
doi = "10.5551/jat.24299"
}

Živković, M., Đurić, T., Stojković, L. S., Jovanović, I. G., Končar, I., Davidović, L., Veljković, N. V., Alavantić, D.,& Stanković, A.. (2015). CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results. in Journal of Atherosclerosis and Thrombosis, 22(1), 10-20.
https://doi.org/10.5551/jat.24299

Živković M, Đurić T, Stojković LS, Jovanović IG, Končar I, Davidović L, Veljković NV, Alavantić D, Stanković A. CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results. in Journal of Atherosclerosis and Thrombosis. 2015;22(1):10-20.
doi:10.5551/jat.24299 .

Živković, Maja, Đurić, Tamara, Stojković, Ljiljana S., Jovanović, Ivan G., Končar, Igor, Davidović, Lazar, Veljković, Nevena V., Alavantić, Dragan, Stanković, Aleksandra, "CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results" in Journal of Atherosclerosis and Thrombosis, 22, no. 1 (2015):10-20,
https://doi.org/10.5551/jat.24299 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Kolaković, Ana
AU  - Đurđević, Vladimir
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5869
AB  - Glutathione S-transferases (GSTs) carry out a wide range of functions in cells, such as detoxification of endogenous compounds, removal of reactive oxygen species, and even catalysis of reactions in metabolic pathways beyond detoxification. Based on previous research, GSTM1 and GSTT1 might modify the risk of atherosclerosis. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the occurrence of carotid plaque (CP); and biochemical parameters of oxidative stress, lipid profile and inflammation, in 346 consecutive patients with advanced atherosclerosis that underwent endarterectomy. A multiplex polymerase chain reaction (PCR) method was used to detect the deletions in GSTM1 and GSTT1 genes in the genomic DNA in 346 patients and 330 controls. The adjusted OR for CP presence (adjusted for age, gender, smoking, hypertension, BMI, HDLC, TG) was 0.24, 95 %CI 0.08-0.7, p LT 0.01 for GSTT1 null and 1.13, 95 %CI 0.62-2.07, p = 0.7 for GSTM1 null genotype. We found significantly lower plasma lipoprotein (a) (Lp(a)) levels in GSTT1 null compared to wild-type genotype carriers in patient group (20.68 +/- A 26.02 mg/dl vs. 40.66 +/- A 42.89 mg/dl, mean +/- A SD, p = 0.04). The serum interleukin-6 (IL-6) values were significantly influenced by both GST polymorphisms in patients with CP. Our results, showing the significant reduction of GSTT1 deletions in patients with CP, suggest involvement of GSTs in carotid atherosclerosis. This study shows additional view of the possible role of GSTs in advanced chronic inflammatory disease of vascular system, but the confirmation in a larger studies in different populations are needed.
T2  - Molecular Biology Reports
T1  - Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters
VL  - 41
IS  - 2
SP  - 1157
EP  - 1164
DO  - 10.1007/s11033-013-2962-z
ER  - 

@article{
author = "Živković, Maja and Stanković, Aleksandra and Đurić, Tamara and Končar, Igor and Kolaković, Ana and Đurđević, Vladimir and Davidović, Lazar and Alavantić, Dragan",
year = "2014",
abstract = "Glutathione S-transferases (GSTs) carry out a wide range of functions in cells, such as detoxification of endogenous compounds, removal of reactive oxygen species, and even catalysis of reactions in metabolic pathways beyond detoxification. Based on previous research, GSTM1 and GSTT1 might modify the risk of atherosclerosis. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the occurrence of carotid plaque (CP); and biochemical parameters of oxidative stress, lipid profile and inflammation, in 346 consecutive patients with advanced atherosclerosis that underwent endarterectomy. A multiplex polymerase chain reaction (PCR) method was used to detect the deletions in GSTM1 and GSTT1 genes in the genomic DNA in 346 patients and 330 controls. The adjusted OR for CP presence (adjusted for age, gender, smoking, hypertension, BMI, HDLC, TG) was 0.24, 95 %CI 0.08-0.7, p LT 0.01 for GSTT1 null and 1.13, 95 %CI 0.62-2.07, p = 0.7 for GSTM1 null genotype. We found significantly lower plasma lipoprotein (a) (Lp(a)) levels in GSTT1 null compared to wild-type genotype carriers in patient group (20.68 +/- A 26.02 mg/dl vs. 40.66 +/- A 42.89 mg/dl, mean +/- A SD, p = 0.04). The serum interleukin-6 (IL-6) values were significantly influenced by both GST polymorphisms in patients with CP. Our results, showing the significant reduction of GSTT1 deletions in patients with CP, suggest involvement of GSTs in carotid atherosclerosis. This study shows additional view of the possible role of GSTs in advanced chronic inflammatory disease of vascular system, but the confirmation in a larger studies in different populations are needed.",
journal = "Molecular Biology Reports",
title = "Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters",
volume = "41",
number = "2",
pages = "1157-1164",
doi = "10.1007/s11033-013-2962-z"
}

Živković, M., Stanković, A., Đurić, T., Končar, I., Kolaković, A., Đurđević, V., Davidović, L.,& Alavantić, D.. (2014). Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters. in Molecular Biology Reports, 41(2), 1157-1164.
https://doi.org/10.1007/s11033-013-2962-z

Živković M, Stanković A, Đurić T, Končar I, Kolaković A, Đurđević V, Davidović L, Alavantić D. Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters. in Molecular Biology Reports. 2014;41(2):1157-1164.
doi:10.1007/s11033-013-2962-z .

Živković, Maja, Stanković, Aleksandra, Đurić, Tamara, Končar, Igor, Kolaković, Ana, Đurđević, Vladimir, Davidović, Lazar, Alavantić, Dragan, "Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters" in Molecular Biology Reports, 41, no. 2 (2014):1157-1164,
https://doi.org/10.1007/s11033-013-2962-z . .

TY  - JOUR
AU  - Zurnic, Irena
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Stanković, Aleksandra
AU  - Dinčić, Dragan
AU  - Živković, Maja
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5961
AB  - Background/Aim. Atherosclerosis is still the leading cause of death in Western world. Development of atherosclerotic plaque involves accumulation of inflammatory cells, lipids, smooth muscle cells and extracellular matrix proteins in the intima of the vascular wall. Apolipoprotein E participates in the transport of exogenous cholesterol, endogenously synthesized lipids and triglycerides in the organism. Apolipoprotein E gene has been identified as one of the candidate genes for atherosclerosis. Previous studies in different populations have clearly implicated apolipoprotein E genetic variation (epsilon polymorphisms) as a major modulator of low density lipoprotein cholesterol levels. Data considering apolipoprotein E polymorphisrns in relation to carotid atherosclerosis gave results that are not in full compliance. The aim of present study was to investigate the apolipoprotein E polymorphisms in association with carotid plaque presence, apolipoprotein E and lipid serum levels in patients with carotid atherosclerosis from Serbia. Methods. The study group enrolled 495 participants: 285 controls and 210 consecutive patients with carotid atherosclerosis who underwent carotid endarterectomy. Genotyping of apolipoprotein E polymorphisms were done using polymerase chain reaction and restriction fragment length polymorphism methods. Results. Patients had significantly decreased frequency of the epsilon 2 allele compared to controls. Patients who carry at least one epsilon 2 allele had a significantly higher level of serum apolipoprotein E and significantly lower low density lipoprotein cholesterol levels compared to those who do not carry this allele. Conclusion. Our results suggest protective effect of apolipoprotein E epsilon 2 allele on susceptibility for carotid plaque presence as well as low density lipoprotein cholesterol lowering effect in Serbian patients with carotid atherosclerosis. Further research of multiple gene and environmental factors that contribute to the appearance and the progression of atherosclerosis should be continued with respect to different populations.
T2  - Vojnosanitetski pregled
T1  - Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis
VL  - 71
IS  - 4
SP  - 362
EP  - 367
DO  - 10.2298/VSP1404362Z
ER  - 

@article{
author = "Zurnic, Irena and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra and Dinčić, Dragan and Živković, Maja",
year = "2014",
abstract = "Background/Aim. Atherosclerosis is still the leading cause of death in Western world. Development of atherosclerotic plaque involves accumulation of inflammatory cells, lipids, smooth muscle cells and extracellular matrix proteins in the intima of the vascular wall. Apolipoprotein E participates in the transport of exogenous cholesterol, endogenously synthesized lipids and triglycerides in the organism. Apolipoprotein E gene has been identified as one of the candidate genes for atherosclerosis. Previous studies in different populations have clearly implicated apolipoprotein E genetic variation (epsilon polymorphisms) as a major modulator of low density lipoprotein cholesterol levels. Data considering apolipoprotein E polymorphisrns in relation to carotid atherosclerosis gave results that are not in full compliance. The aim of present study was to investigate the apolipoprotein E polymorphisms in association with carotid plaque presence, apolipoprotein E and lipid serum levels in patients with carotid atherosclerosis from Serbia. Methods. The study group enrolled 495 participants: 285 controls and 210 consecutive patients with carotid atherosclerosis who underwent carotid endarterectomy. Genotyping of apolipoprotein E polymorphisms were done using polymerase chain reaction and restriction fragment length polymorphism methods. Results. Patients had significantly decreased frequency of the epsilon 2 allele compared to controls. Patients who carry at least one epsilon 2 allele had a significantly higher level of serum apolipoprotein E and significantly lower low density lipoprotein cholesterol levels compared to those who do not carry this allele. Conclusion. Our results suggest protective effect of apolipoprotein E epsilon 2 allele on susceptibility for carotid plaque presence as well as low density lipoprotein cholesterol lowering effect in Serbian patients with carotid atherosclerosis. Further research of multiple gene and environmental factors that contribute to the appearance and the progression of atherosclerosis should be continued with respect to different populations.",
journal = "Vojnosanitetski pregled",
title = "Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis",
volume = "71",
number = "4",
pages = "362-367",
doi = "10.2298/VSP1404362Z"
}

Zurnic, I., Đurić, T., Končar, I., Stanković, A., Dinčić, D.,& Živković, M.. (2014). Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis. in Vojnosanitetski pregled, 71(4), 362-367.
https://doi.org/10.2298/VSP1404362Z

Zurnic I, Đurić T, Končar I, Stanković A, Dinčić D, Živković M. Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis. in Vojnosanitetski pregled. 2014;71(4):362-367.
doi:10.2298/VSP1404362Z .

Zurnic, Irena, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, Dinčić, Dragan, Živković, Maja, "Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis" in Vojnosanitetski pregled, 71, no. 4 (2014):362-367,
https://doi.org/10.2298/VSP1404362Z . .

TY  - CONF
AU  - Životić, Ivan
AU  - Đorđević, Ana
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12811
AB  - Genome wide association studies have recognized the 9p21 rs10757278 polymorphism as a significant independent genetic prognostic marker for coronary artery disease. The aim of this study was to explore possible association of the rs10757278 polymorphism with advanced carotid atherosclerosis (CA) in the population of Serbia. The study group included 147 controls and 428 patients consecutively admitted for carotid endarterectomy. 9p21 rs10757278 polymorphism was genotyped using TaqMan technology on 7500 ABI Real Time PCR. There was no significant association of this polymorphism and CA, either in study group overall or in males. The GG genotype, according to recessive model of inheritance (AA+AG vs. GG), was significantly associated with advanced CA in females only (OR=2.15, 95% CI 1.07- 4.29, p=0.03). Preliminary results in this study suggest that rs10757278 GG genotype might be a significant predictive sex-specific marker for advanced CA in the population of Serbia.
PB  - Belgrade : Serbian Physiological Society
C3  - V Congress of the Serbian Genetic Society : Book of abstracts
T1  - Polymorphism rs10757278 in 9p21 region is associated with severe carotid atherosclerosis in sex specific manner: Preliminary results
SP  - 56
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12811
ER  - 

@conference{
author = "Životić, Ivan and Đorđević, Ana and Đurić, Tamara and Končar, Igor and Alavantić, Dragan and Stanković, Aleksandra and Živković, Maja",
year = "2014",
abstract = "Genome wide association studies have recognized the 9p21 rs10757278 polymorphism as a significant independent genetic prognostic marker for coronary artery disease. The aim of this study was to explore possible association of the rs10757278 polymorphism with advanced carotid atherosclerosis (CA) in the population of Serbia. The study group included 147 controls and 428 patients consecutively admitted for carotid endarterectomy. 9p21 rs10757278 polymorphism was genotyped using TaqMan technology on 7500 ABI Real Time PCR. There was no significant association of this polymorphism and CA, either in study group overall or in males. The GG genotype, according to recessive model of inheritance (AA+AG vs. GG), was significantly associated with advanced CA in females only (OR=2.15, 95% CI 1.07- 4.29, p=0.03). Preliminary results in this study suggest that rs10757278 GG genotype might be a significant predictive sex-specific marker for advanced CA in the population of Serbia.",
publisher = "Belgrade : Serbian Physiological Society",
journal = "V Congress of the Serbian Genetic Society : Book of abstracts",
title = "Polymorphism rs10757278 in 9p21 region is associated with severe carotid atherosclerosis in sex specific manner: Preliminary results",
pages = "56-56",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12811"
}

Životić, I., Đorđević, A., Đurić, T., Končar, I., Alavantić, D., Stanković, A.,& Živković, M.. (2014). Polymorphism rs10757278 in 9p21 region is associated with severe carotid atherosclerosis in sex specific manner: Preliminary results. in V Congress of the Serbian Genetic Society : Book of abstracts
Belgrade : Serbian Physiological Society., 56-56.
https://hdl.handle.net/21.15107/rcub_vinar_12811

Životić I, Đorđević A, Đurić T, Končar I, Alavantić D, Stanković A, Živković M. Polymorphism rs10757278 in 9p21 region is associated with severe carotid atherosclerosis in sex specific manner: Preliminary results. in V Congress of the Serbian Genetic Society : Book of abstracts. 2014;:56-56.
https://hdl.handle.net/21.15107/rcub_vinar_12811 .

Životić, Ivan, Đorđević, Ana, Đurić, Tamara, Končar, Igor, Alavantić, Dragan, Stanković, Aleksandra, Živković, Maja, "Polymorphism rs10757278 in 9p21 region is associated with severe carotid atherosclerosis in sex specific manner: Preliminary results" in V Congress of the Serbian Genetic Society : Book of abstracts (2014):56-56,
https://hdl.handle.net/21.15107/rcub_vinar_12811 .

TY  - CONF
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Đorđević, Ana
AU  - Životić, Ivan
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12814
PB  - European Atherosclerosis Society
C3  - 82nd Congress of the European Atherosclerosis Society (EAS 2014) : Book of abstracts
T1  - The 9p21 as a potential risk factor for cad comorbidity in advanced carotid atherosclerosis: preliminary study
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12814
ER  - 

@conference{
author = "Živković, Maja and Đurić, Tamara and Đorđević, Ana and Životić, Ivan and Končar, Igor and Davidović, Lazar and Alavantić, Dragan and Stanković, Aleksandra",
year = "2014",
publisher = "European Atherosclerosis Society",
journal = "82nd Congress of the European Atherosclerosis Society (EAS 2014) : Book of abstracts",
title = "The 9p21 as a potential risk factor for cad comorbidity in advanced carotid atherosclerosis: preliminary study",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12814"
}

Živković, M., Đurić, T., Đorđević, A., Životić, I., Končar, I., Davidović, L., Alavantić, D.,& Stanković, A.. (2014). The 9p21 as a potential risk factor for cad comorbidity in advanced carotid atherosclerosis: preliminary study. in 82nd Congress of the European Atherosclerosis Society (EAS 2014) : Book of abstracts
European Atherosclerosis Society..
https://hdl.handle.net/21.15107/rcub_vinar_12814

Živković M, Đurić T, Đorđević A, Životić I, Končar I, Davidović L, Alavantić D, Stanković A. The 9p21 as a potential risk factor for cad comorbidity in advanced carotid atherosclerosis: preliminary study. in 82nd Congress of the European Atherosclerosis Society (EAS 2014) : Book of abstracts. 2014;.
https://hdl.handle.net/21.15107/rcub_vinar_12814 .

Živković, Maja, Đurić, Tamara, Đorđević, Ana, Životić, Ivan, Končar, Igor, Davidović, Lazar, Alavantić, Dragan, Stanković, Aleksandra, "The 9p21 as a potential risk factor for cad comorbidity in advanced carotid atherosclerosis: preliminary study" in 82nd Congress of the European Atherosclerosis Society (EAS 2014) : Book of abstracts (2014),
https://hdl.handle.net/21.15107/rcub_vinar_12814 .

TY  - CONF
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Končar, Igor
AU  - Jovanović, Ivan G.
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5821
C3  - Cerebrovascular Diseases
T1  - The angiotensin II type 2 receptor-1332 A/G gene polymorphism (rs1403543) is associated with stroke in males patients with carotid atherosclerosis
VL  - 35
SP  - 536
EP  - 536
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5821
ER  - 

@conference{
author = "Kolaković, Ana and Živković, Maja and Končar, Igor and Jovanović, Ivan G. and Đurić, Tamara and Stanković, Aleksandra",
year = "2013",
journal = "Cerebrovascular Diseases",
title = "The angiotensin II type 2 receptor-1332 A/G gene polymorphism (rs1403543) is associated with stroke in males patients with carotid atherosclerosis",
volume = "35",
pages = "536-536",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5821"
}

Kolaković, A., Živković, M., Končar, I., Jovanović, I. G., Đurić, T.,& Stanković, A.. (2013). The angiotensin II type 2 receptor-1332 A/G gene polymorphism (rs1403543) is associated with stroke in males patients with carotid atherosclerosis. in Cerebrovascular Diseases, 35, 536-536.
https://hdl.handle.net/21.15107/rcub_vinar_5821

Kolaković A, Živković M, Končar I, Jovanović IG, Đurić T, Stanković A. The angiotensin II type 2 receptor-1332 A/G gene polymorphism (rs1403543) is associated with stroke in males patients with carotid atherosclerosis. in Cerebrovascular Diseases. 2013;35:536-536.
https://hdl.handle.net/21.15107/rcub_vinar_5821 .

Kolaković, Ana, Živković, Maja, Končar, Igor, Jovanović, Ivan G., Đurić, Tamara, Stanković, Aleksandra, "The angiotensin II type 2 receptor-1332 A/G gene polymorphism (rs1403543) is associated with stroke in males patients with carotid atherosclerosis" in Cerebrovascular Diseases, 35 (2013):536-536,
https://hdl.handle.net/21.15107/rcub_vinar_5821 .

TY  - JOUR
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Radak, Đorđe J.
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Dinčić, Dragan
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4719
AB  - Introduction: The ACE I/D polymorphism was mostly investigated in association with intima-media thickness, rarely with severe atherosclerotic phenotype. Materials and methods: We investigated the association of I/D polymorphism with severe carotid atherosclerosis (CA) (stenosis GT 70%) in asymptomatic and symptomatic patients undergoing carotid endarterectomy. The 504 patients subjected to endarterectomy and 492 healthy controls from a population in Serbia were investigated as a case-control study. Results: The univariate logistic regression analysis revealed ACE DD as a significant risk factor for severe CA (odds ratio [OR] = 1.3, 95% confidence interval [CI] 1.0-1.7, p = 0.04). After adjustment for the common risk factors (age, hypertension, smoking, and HDL) ACE was no longer significant. However, we found a significant independent influence of DD genotype on plaque presence in a normotensive subgroup of patients (OR 1.8, CI 1.2-3.0, p = 0.01, corrected for multiple testing). In symptomatic patients D allele carriers were significantly more frequent compared with asymptomatic patients (OR 1.6 CI 1.0-2.6, p = 0.05). Conclusions: Our data suggests that ACE I/D is not an independent risk factor for severe CA. On the other hand, a significant independent genetic influence of ACE I/D appeared in normotensive and symptomatic patients with severe CA. This should be considered in further research toward resolving the complex genetic background of severe CA phenotype.
T2  - Journal of the Renin-Angiotensin-Aldosterone System
T1  - The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy
VL  - 13
IS  - 1
SP  - 141
EP  - 147
DO  - 10.1177/1470320311423271
ER  - 

@article{
author = "Kolaković, Ana and Živković, Maja and Radak, Đorđe J. and Đurić, Tamara and Končar, Igor and Davidović, Lazar and Dinčić, Dragan and Alavantić, Dragan and Stanković, Aleksandra",
year = "2012",
abstract = "Introduction: The ACE I/D polymorphism was mostly investigated in association with intima-media thickness, rarely with severe atherosclerotic phenotype. Materials and methods: We investigated the association of I/D polymorphism with severe carotid atherosclerosis (CA) (stenosis GT 70%) in asymptomatic and symptomatic patients undergoing carotid endarterectomy. The 504 patients subjected to endarterectomy and 492 healthy controls from a population in Serbia were investigated as a case-control study. Results: The univariate logistic regression analysis revealed ACE DD as a significant risk factor for severe CA (odds ratio [OR] = 1.3, 95% confidence interval [CI] 1.0-1.7, p = 0.04). After adjustment for the common risk factors (age, hypertension, smoking, and HDL) ACE was no longer significant. However, we found a significant independent influence of DD genotype on plaque presence in a normotensive subgroup of patients (OR 1.8, CI 1.2-3.0, p = 0.01, corrected for multiple testing). In symptomatic patients D allele carriers were significantly more frequent compared with asymptomatic patients (OR 1.6 CI 1.0-2.6, p = 0.05). Conclusions: Our data suggests that ACE I/D is not an independent risk factor for severe CA. On the other hand, a significant independent genetic influence of ACE I/D appeared in normotensive and symptomatic patients with severe CA. This should be considered in further research toward resolving the complex genetic background of severe CA phenotype.",
journal = "Journal of the Renin-Angiotensin-Aldosterone System",
title = "The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy",
volume = "13",
number = "1",
pages = "141-147",
doi = "10.1177/1470320311423271"
}

Kolaković, A., Živković, M., Radak, Đ. J., Đurić, T., Končar, I., Davidović, L., Dinčić, D., Alavantić, D.,& Stanković, A.. (2012). The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy. in Journal of the Renin-Angiotensin-Aldosterone System, 13(1), 141-147.
https://doi.org/10.1177/1470320311423271

Kolaković A, Živković M, Radak ĐJ, Đurić T, Končar I, Davidović L, Dinčić D, Alavantić D, Stanković A. The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy. in Journal of the Renin-Angiotensin-Aldosterone System. 2012;13(1):141-147.
doi:10.1177/1470320311423271 .

Kolaković, Ana, Živković, Maja, Radak, Đorđe J., Đurić, Tamara, Končar, Igor, Davidović, Lazar, Dinčić, Dragan, Alavantić, Dragan, Stanković, Aleksandra, "The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy" in Journal of the Renin-Angiotensin-Aldosterone System, 13, no. 1 (2012):141-147,
https://doi.org/10.1177/1470320311423271 . .

TY  - JOUR
AU  - Đurić, Tamara
AU  - Stojković, Ljiljana S.
AU  - Živković, Maja
AU  - Končar, Igor
AU  - Stanković, Aleksandra
AU  - Đorđević, Ana
AU  - Alavantić, Dragan
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5161
AB  - Objectives: Matrix metalloproteinase (MMP)-1 degrades fibrillar collagens suggesting important role in vascular remodeling. Data about MMP-1 promoter polymorphisms and carotid atherosclerosis (CA) are scarce. The aim of this study was to evaluate association of MMP-1 genotypes/haplotypes with carotid plaque (CP) presence in Serbian population. Design and methods: Study enrolled a total of 702 participants: 274 controls and 428 consecutive patients with CA who underwent carotid endarterectomy. MMP-1 polymorphisms -1607 1G/2G, -519 A/G and -340 T/C were genotyped by PCR and RFLP methods. Results: Individuals carrying MMP-1 - 1607 2G allele had significantly increased allele dose-dependent risk for CP presence (1G1G vs. 1G2G vs. 2G2G; OR = 1; OR = 1.87 95% CI 1.29-2.07; OR= 3.49 95% CI 1.67-7.30. p = 0.0009, respectively). Compared to the referent haplotype 2G_ (1607)-T_ (340)-A_(519), the haplotypes 1G_(1607)-T_(340)-A_(519), 1G-(1607)-T_(340)-G_519 and 2G_(1607)-C_(340)-A_(519) had statistically significant protective effect on CP presence (OR = 0.41, 95% CI 0.29-0.81, p = 0.01; OR = 0.56, 95% CI 0.44-0.89, p = 0.01: OR = 0.43, 95% CI 0.27-0.86, p = 0.02, respectively). Conclusions: MMP-1 - 1607 G/2G polymorphism solely and specific haplotypes of three analyzed promoter polymorphisms are significantly and independently associated with occurrence of CP. Replication studies in other populations are needed. (c) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
T2  - Clinical Biochemistry
T1  - Matrix metalloproteinase-1 promoter genotypes and haplotypes are associated with carotid plaque presence
VL  - 45
IS  - 16-17
SP  - 1353
EP  - 1356
DO  - 10.1016/j.clinbiochem.2012.05.032
ER  - 

@article{
author = "Đurić, Tamara and Stojković, Ljiljana S. and Živković, Maja and Končar, Igor and Stanković, Aleksandra and Đorđević, Ana and Alavantić, Dragan",
year = "2012",
abstract = "Objectives: Matrix metalloproteinase (MMP)-1 degrades fibrillar collagens suggesting important role in vascular remodeling. Data about MMP-1 promoter polymorphisms and carotid atherosclerosis (CA) are scarce. The aim of this study was to evaluate association of MMP-1 genotypes/haplotypes with carotid plaque (CP) presence in Serbian population. Design and methods: Study enrolled a total of 702 participants: 274 controls and 428 consecutive patients with CA who underwent carotid endarterectomy. MMP-1 polymorphisms -1607 1G/2G, -519 A/G and -340 T/C were genotyped by PCR and RFLP methods. Results: Individuals carrying MMP-1 - 1607 2G allele had significantly increased allele dose-dependent risk for CP presence (1G1G vs. 1G2G vs. 2G2G; OR = 1; OR = 1.87 95% CI 1.29-2.07; OR= 3.49 95% CI 1.67-7.30. p = 0.0009, respectively). Compared to the referent haplotype 2G_ (1607)-T_ (340)-A_(519), the haplotypes 1G_(1607)-T_(340)-A_(519), 1G-(1607)-T_(340)-G_519 and 2G_(1607)-C_(340)-A_(519) had statistically significant protective effect on CP presence (OR = 0.41, 95% CI 0.29-0.81, p = 0.01; OR = 0.56, 95% CI 0.44-0.89, p = 0.01: OR = 0.43, 95% CI 0.27-0.86, p = 0.02, respectively). Conclusions: MMP-1 - 1607 G/2G polymorphism solely and specific haplotypes of three analyzed promoter polymorphisms are significantly and independently associated with occurrence of CP. Replication studies in other populations are needed. (c) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.",
journal = "Clinical Biochemistry",
title = "Matrix metalloproteinase-1 promoter genotypes and haplotypes are associated with carotid plaque presence",
volume = "45",
number = "16-17",
pages = "1353-1356",
doi = "10.1016/j.clinbiochem.2012.05.032"
}

Đurić, T., Stojković, L. S., Živković, M., Končar, I., Stanković, A., Đorđević, A.,& Alavantić, D.. (2012). Matrix metalloproteinase-1 promoter genotypes and haplotypes are associated with carotid plaque presence. in Clinical Biochemistry, 45(16-17), 1353-1356.
https://doi.org/10.1016/j.clinbiochem.2012.05.032

Đurić T, Stojković LS, Živković M, Končar I, Stanković A, Đorđević A, Alavantić D. Matrix metalloproteinase-1 promoter genotypes and haplotypes are associated with carotid plaque presence. in Clinical Biochemistry. 2012;45(16-17):1353-1356.
doi:10.1016/j.clinbiochem.2012.05.032 .

Đurić, Tamara, Stojković, Ljiljana S., Živković, Maja, Končar, Igor, Stanković, Aleksandra, Đorđević, Ana, Alavantić, Dragan, "Matrix metalloproteinase-1 promoter genotypes and haplotypes are associated with carotid plaque presence" in Clinical Biochemistry, 45, no. 16-17 (2012):1353-1356,
https://doi.org/10.1016/j.clinbiochem.2012.05.032 . .

TY  - CONF
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Radak, Đorđe J.
AU  - Končar, Igor
AU  - Đurić, Tamara
AU  - Davidović, L.
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6939
C3  - Atherosclerosis Supplements
T1  - Association of Angiotensin Ii Type 1 Receptor+1166 A/C Gene Polymorphism with Human Carotid Plaque Vulnerability
VL  - 12
IS  - 1
SP  - 111
EP  - 111
DO  - 10.1016/S1567-5688(11)70526-8
ER  - 

@conference{
author = "Kolaković, Ana and Živković, Maja and Radak, Đorđe J. and Končar, Igor and Đurić, Tamara and Davidović, L. and Alavantić, Dragan and Stanković, Aleksandra",
year = "2011",
journal = "Atherosclerosis Supplements",
title = "Association of Angiotensin Ii Type 1 Receptor+1166 A/C Gene Polymorphism with Human Carotid Plaque Vulnerability",
volume = "12",
number = "1",
pages = "111-111",
doi = "10.1016/S1567-5688(11)70526-8"
}

Kolaković, A., Živković, M., Radak, Đ. J., Končar, I., Đurić, T., Davidović, L., Alavantić, D.,& Stanković, A.. (2011). Association of Angiotensin Ii Type 1 Receptor+1166 A/C Gene Polymorphism with Human Carotid Plaque Vulnerability. in Atherosclerosis Supplements, 12(1), 111-111.
https://doi.org/10.1016/S1567-5688(11)70526-8

Kolaković A, Živković M, Radak ĐJ, Končar I, Đurić T, Davidović L, Alavantić D, Stanković A. Association of Angiotensin Ii Type 1 Receptor+1166 A/C Gene Polymorphism with Human Carotid Plaque Vulnerability. in Atherosclerosis Supplements. 2011;12(1):111-111.
doi:10.1016/S1567-5688(11)70526-8 .

Kolaković, Ana, Živković, Maja, Radak, Đorđe J., Končar, Igor, Đurić, Tamara, Davidović, L., Alavantić, Dragan, Stanković, Aleksandra, "Association of Angiotensin Ii Type 1 Receptor+1166 A/C Gene Polymorphism with Human Carotid Plaque Vulnerability" in Atherosclerosis Supplements, 12, no. 1 (2011):111-111,
https://doi.org/10.1016/S1567-5688(11)70526-8 . .

TY  - CONF
AU  - Kolaković, Ana
AU  - Končar, Igor
AU  - Stančić, O.
AU  - Davidović, L.
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6940
C3  - Atherosclerosis Supplements
T1  - The M62i Gene Polymorphism in P-Selectin Glycoprotein Ligand (Psgl-1) with Carotid Atherosclerosis: a Preliminar Study
VL  - 12
IS  - 1
SP  - 126
EP  - 126
DO  - 10.1016/S1567-5688(11)70600-6
ER  - 

@conference{
author = "Kolaković, Ana and Končar, Igor and Stančić, O. and Davidović, L. and Alavantić, Dragan and Stanković, Aleksandra and Živković, Maja",
year = "2011",
journal = "Atherosclerosis Supplements",
title = "The M62i Gene Polymorphism in P-Selectin Glycoprotein Ligand (Psgl-1) with Carotid Atherosclerosis: a Preliminar Study",
volume = "12",
number = "1",
pages = "126-126",
doi = "10.1016/S1567-5688(11)70600-6"
}

Kolaković, A., Končar, I., Stančić, O., Davidović, L., Alavantić, D., Stanković, A.,& Živković, M.. (2011). The M62i Gene Polymorphism in P-Selectin Glycoprotein Ligand (Psgl-1) with Carotid Atherosclerosis: a Preliminar Study. in Atherosclerosis Supplements, 12(1), 126-126.
https://doi.org/10.1016/S1567-5688(11)70600-6

Kolaković A, Končar I, Stančić O, Davidović L, Alavantić D, Stanković A, Živković M. The M62i Gene Polymorphism in P-Selectin Glycoprotein Ligand (Psgl-1) with Carotid Atherosclerosis: a Preliminar Study. in Atherosclerosis Supplements. 2011;12(1):126-126.
doi:10.1016/S1567-5688(11)70600-6 .

Kolaković, Ana, Končar, Igor, Stančić, O., Davidović, L., Alavantić, Dragan, Stanković, Aleksandra, Živković, Maja, "The M62i Gene Polymorphism in P-Selectin Glycoprotein Ligand (Psgl-1) with Carotid Atherosclerosis: a Preliminar Study" in Atherosclerosis Supplements, 12, no. 1 (2011):126-126,
https://doi.org/10.1016/S1567-5688(11)70600-6 . .

TY  - CONF
AU  - Živković, Maja
AU  - Popović, Milan
AU  - Stojković, Ljiljana S.
AU  - Radak, Đorđe J.
AU  - Končar, Igor
AU  - Davidovic, L.
AU  - Kolaković, Ana
AU  - Stanković, Aleksandra
AU  - Alavantić, Dragan
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6941
C3  - Atherosclerosis Supplements
T1  - The Association of Cxcl16 A181v Gene Polymorphism with Carotid Atherosclerosis in Patients Subjected to Endaterectomy
VL  - 12
IS  - 1
SP  - 153
EP  - 153
DO  - 10.1016/S1567-5688(11)70729-2
ER  - 

@conference{
author = "Živković, Maja and Popović, Milan and Stojković, Ljiljana S. and Radak, Đorđe J. and Končar, Igor and Davidovic, L. and Kolaković, Ana and Stanković, Aleksandra and Alavantić, Dragan",
year = "2011",
journal = "Atherosclerosis Supplements",
title = "The Association of Cxcl16 A181v Gene Polymorphism with Carotid Atherosclerosis in Patients Subjected to Endaterectomy",
volume = "12",
number = "1",
pages = "153-153",
doi = "10.1016/S1567-5688(11)70729-2"
}

Živković, M., Popović, M., Stojković, L. S., Radak, Đ. J., Končar, I., Davidovic, L., Kolaković, A., Stanković, A.,& Alavantić, D.. (2011). The Association of Cxcl16 A181v Gene Polymorphism with Carotid Atherosclerosis in Patients Subjected to Endaterectomy. in Atherosclerosis Supplements, 12(1), 153-153.
https://doi.org/10.1016/S1567-5688(11)70729-2

Živković M, Popović M, Stojković LS, Radak ĐJ, Končar I, Davidovic L, Kolaković A, Stanković A, Alavantić D. The Association of Cxcl16 A181v Gene Polymorphism with Carotid Atherosclerosis in Patients Subjected to Endaterectomy. in Atherosclerosis Supplements. 2011;12(1):153-153.
doi:10.1016/S1567-5688(11)70729-2 .

Živković, Maja, Popović, Milan, Stojković, Ljiljana S., Radak, Đorđe J., Končar, Igor, Davidovic, L., Kolaković, Ana, Stanković, Aleksandra, Alavantić, Dragan, "The Association of Cxcl16 A181v Gene Polymorphism with Carotid Atherosclerosis in Patients Subjected to Endaterectomy" in Atherosclerosis Supplements, 12, no. 1 (2011):153-153,
https://doi.org/10.1016/S1567-5688(11)70729-2 . .

TY  - JOUR
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Končar, Igor
AU  - Radak, Đorđe J.
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4641
AB  - Objective: Matrix metalloproteinases (MMPs) are involved in the remodeling of the extracellular matrix in the arterial wall. Collagen I is associated with vascular smooth muscle cell (VSMC) migration and monocyte differentiation. MMP-8 is expressed in atherosclerotic plaque and preferentially cleaves collagen type I. The aim of this study was to investigate the associations of two MMP-8 promoter polymorphisms, rs11225395 (-799 C/T) and rs1320632 (-381 A/G), with carotid plaque occurrence, and the influence of these polymorphisms on MMP-8 mRNA expression in plaque tissue. Methods: The study included a total of 766 participants: 277 controls and 489 patients with carotid atherosclerosis undergoing endarterectomy. The two investigated polymorphisms were genotyped by PCR-RFLP. The gene expression analysis was performed by real-time PCR. Results: In females only, a significantly higher frequency of the -381G allele was found in patients with carotid atherosclerosis compared to controls (OR, 1.7; 95% CI 1.1-2.9; p = 0.001). Significant up-regulation of MMP-8 gene expression was observed in patients carrying the -381G allele compared to those with the AA genotype (mean factor, 3.54; SE. range, 0.643-19.551; p = 0.007). Carotid plaque tissue of the haplotype G(-381)T(-799) showed a significantly higher mRNA level compared with the reference A(-381)C(-799) haplotype (p = 0.003). Conclusion: Our preliminary results indicate that MMP-8 -381A/G and -799 C/T gene polymorphisms could be risk factors for carotid atherosclerosis. Further validation and functional studies are needed to establish the potential regulatory role of these polymorphisms and their impact on susceptibility to carotid atherosclerosis. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
T2  - Atherosclerosis
T1  - Association of MMP-8 promoter gene polymorphisms with carotid atherosclerosis: Preliminary study
VL  - 219
IS  - 2
SP  - 673
EP  - 678
DO  - 10.1016/j.atherosclerosis.2011.08.025
ER  - 

@article{
author = "Đurić, Tamara and Stanković, Aleksandra and Končar, Igor and Radak, Đorđe J. and Davidović, Lazar and Alavantić, Dragan and Živković, Maja",
year = "2011",
abstract = "Objective: Matrix metalloproteinases (MMPs) are involved in the remodeling of the extracellular matrix in the arterial wall. Collagen I is associated with vascular smooth muscle cell (VSMC) migration and monocyte differentiation. MMP-8 is expressed in atherosclerotic plaque and preferentially cleaves collagen type I. The aim of this study was to investigate the associations of two MMP-8 promoter polymorphisms, rs11225395 (-799 C/T) and rs1320632 (-381 A/G), with carotid plaque occurrence, and the influence of these polymorphisms on MMP-8 mRNA expression in plaque tissue. Methods: The study included a total of 766 participants: 277 controls and 489 patients with carotid atherosclerosis undergoing endarterectomy. The two investigated polymorphisms were genotyped by PCR-RFLP. The gene expression analysis was performed by real-time PCR. Results: In females only, a significantly higher frequency of the -381G allele was found in patients with carotid atherosclerosis compared to controls (OR, 1.7; 95% CI 1.1-2.9; p = 0.001). Significant up-regulation of MMP-8 gene expression was observed in patients carrying the -381G allele compared to those with the AA genotype (mean factor, 3.54; SE. range, 0.643-19.551; p = 0.007). Carotid plaque tissue of the haplotype G(-381)T(-799) showed a significantly higher mRNA level compared with the reference A(-381)C(-799) haplotype (p = 0.003). Conclusion: Our preliminary results indicate that MMP-8 -381A/G and -799 C/T gene polymorphisms could be risk factors for carotid atherosclerosis. Further validation and functional studies are needed to establish the potential regulatory role of these polymorphisms and their impact on susceptibility to carotid atherosclerosis. (C) 2011 Elsevier Ireland Ltd. All rights reserved.",
journal = "Atherosclerosis",
title = "Association of MMP-8 promoter gene polymorphisms with carotid atherosclerosis: Preliminary study",
volume = "219",
number = "2",
pages = "673-678",
doi = "10.1016/j.atherosclerosis.2011.08.025"
}

Đurić, T., Stanković, A., Končar, I., Radak, Đ. J., Davidović, L., Alavantić, D.,& Živković, M.. (2011). Association of MMP-8 promoter gene polymorphisms with carotid atherosclerosis: Preliminary study. in Atherosclerosis, 219(2), 673-678.
https://doi.org/10.1016/j.atherosclerosis.2011.08.025

Đurić T, Stanković A, Končar I, Radak ĐJ, Davidović L, Alavantić D, Živković M. Association of MMP-8 promoter gene polymorphisms with carotid atherosclerosis: Preliminary study. in Atherosclerosis. 2011;219(2):673-678.
doi:10.1016/j.atherosclerosis.2011.08.025 .

Đurić, Tamara, Stanković, Aleksandra, Končar, Igor, Radak, Đorđe J., Davidović, Lazar, Alavantić, Dragan, Živković, Maja, "Association of MMP-8 promoter gene polymorphisms with carotid atherosclerosis: Preliminary study" in Atherosclerosis, 219, no. 2 (2011):673-678,
https://doi.org/10.1016/j.atherosclerosis.2011.08.025 . .

TY  - JOUR
AU  - Đurić, Tamara
AU  - Umicevic, Masa
AU  - Končar, Igor
AU  - Živković, Maja
AU  - Vasić, Dragan
AU  - Davidović, Lazar
AU  - Stanković, Aleksandra
AU  - Alavantić, Dragan
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3835
T2  - Clinical Chemistry and Laboratory Medicine
T1  - Lack of association between eNOS Glu298Asp gene polymorphism and carotid atherosclerosis in a Serbian population
VL  - 47
IS  - 12
SP  - 1573
EP  - 1575
DO  - 10.1515/CCLM.2009.343
ER  - 

@article{
author = "Đurić, Tamara and Umicevic, Masa and Končar, Igor and Živković, Maja and Vasić, Dragan and Davidović, Lazar and Stanković, Aleksandra and Alavantić, Dragan",
year = "2009",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Lack of association between eNOS Glu298Asp gene polymorphism and carotid atherosclerosis in a Serbian population",
volume = "47",
number = "12",
pages = "1573-1575",
doi = "10.1515/CCLM.2009.343"
}

Đurić, T., Umicevic, M., Končar, I., Živković, M., Vasić, D., Davidović, L., Stanković, A.,& Alavantić, D.. (2009). Lack of association between eNOS Glu298Asp gene polymorphism and carotid atherosclerosis in a Serbian population. in Clinical Chemistry and Laboratory Medicine, 47(12), 1573-1575.
https://doi.org/10.1515/CCLM.2009.343

Đurić T, Umicevic M, Končar I, Živković M, Vasić D, Davidović L, Stanković A, Alavantić D. Lack of association between eNOS Glu298Asp gene polymorphism and carotid atherosclerosis in a Serbian population. in Clinical Chemistry and Laboratory Medicine. 2009;47(12):1573-1575.
doi:10.1515/CCLM.2009.343 .

Đurić, Tamara, Umicevic, Masa, Končar, Igor, Živković, Maja, Vasić, Dragan, Davidović, Lazar, Stanković, Aleksandra, Alavantić, Dragan, "Lack of association between eNOS Glu298Asp gene polymorphism and carotid atherosclerosis in a Serbian population" in Clinical Chemistry and Laboratory Medicine, 47, no. 12 (2009):1573-1575,
https://doi.org/10.1515/CCLM.2009.343 . .