TY  - JOUR
AU  - Radić, Ivan
AU  - Mirić, Mirjana
AU  - Mijović, Milica
AU  - Tatalović, Nikola
AU  - Mitić, Miloš
AU  - Nestorović, Vojkan
AU  - Adžić, Miroslav
AU  - Blagojević, Duško
AU  - Popović, Ljiljana
AU  - Janićijević-Hudomal, Snežana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9412
AB  - Pumpkin seed oil (PSO) possesses a protective potential against liver injury due to the presence of biologically active ingredients. Adult male albino rats were administrated PSO (per os, 2 mL/kg b.w./day) and a 12% ethanol solution in water, ad libitum, with an average intake of 8.14 g of ethanol/kg bw/day for 6 weeks. Congestion, hepatic central vein dilation, portal vein branch dilation, Kupffer cell hyperplasia, fatty liver changes, hepatocyte focal necrosis were observed after daily alcohol intake. All observed changes were reduced when PSO was ingested with ethanol. PSO intake itself induced discrete cellular edema, congestion and slight dilatation of the central and portal vain branches. Chronic ethanol intake elevated catalase (CAT) activity and glutathione reductase (GR) protein expression; concomitant PSO intake had no effect on CAT activity or GR protein expression. PSO intake decreased the activities of GR, glutathione-S-transferase (GST) and xanthine oxidase (XOD) in the liver, probably due to the ingestion of antioxidants. Intake of PSO and ethanol significantly decreased cytosolic superoxide dismutase (SOD1) and increased NF-?B protein expression compared to ethanol intake, suggesting that the protective effects of PSO were mediated by the NF-?B signaling pathway. Our results reveal a therapeutic potential of PSO in alcoholic liver disease.
T2  - Archives of Biological Sciences
T1  - Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption
VL  - 73
IS  - 1
SP  - 123
EP  - 133
DO  - 10.2298/ABS201205008R
ER  - 

@article{
author = "Radić, Ivan and Mirić, Mirjana and Mijović, Milica and Tatalović, Nikola and Mitić, Miloš and Nestorović, Vojkan and Adžić, Miroslav and Blagojević, Duško and Popović, Ljiljana and Janićijević-Hudomal, Snežana",
year = "2021",
abstract = "Pumpkin seed oil (PSO) possesses a protective potential against liver injury due to the presence of biologically active ingredients. Adult male albino rats were administrated PSO (per os, 2 mL/kg b.w./day) and a 12% ethanol solution in water, ad libitum, with an average intake of 8.14 g of ethanol/kg bw/day for 6 weeks. Congestion, hepatic central vein dilation, portal vein branch dilation, Kupffer cell hyperplasia, fatty liver changes, hepatocyte focal necrosis were observed after daily alcohol intake. All observed changes were reduced when PSO was ingested with ethanol. PSO intake itself induced discrete cellular edema, congestion and slight dilatation of the central and portal vain branches. Chronic ethanol intake elevated catalase (CAT) activity and glutathione reductase (GR) protein expression; concomitant PSO intake had no effect on CAT activity or GR protein expression. PSO intake decreased the activities of GR, glutathione-S-transferase (GST) and xanthine oxidase (XOD) in the liver, probably due to the ingestion of antioxidants. Intake of PSO and ethanol significantly decreased cytosolic superoxide dismutase (SOD1) and increased NF-?B protein expression compared to ethanol intake, suggesting that the protective effects of PSO were mediated by the NF-?B signaling pathway. Our results reveal a therapeutic potential of PSO in alcoholic liver disease.",
journal = "Archives of Biological Sciences",
title = "Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption",
volume = "73",
number = "1",
pages = "123-133",
doi = "10.2298/ABS201205008R"
}

Radić, I., Mirić, M., Mijović, M., Tatalović, N., Mitić, M., Nestorović, V., Adžić, M., Blagojević, D., Popović, L.,& Janićijević-Hudomal, S.. (2021). Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption. in Archives of Biological Sciences, 73(1), 123-133.
https://doi.org/10.2298/ABS201205008R

Radić I, Mirić M, Mijović M, Tatalović N, Mitić M, Nestorović V, Adžić M, Blagojević D, Popović L, Janićijević-Hudomal S. Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption. in Archives of Biological Sciences. 2021;73(1):123-133.
doi:10.2298/ABS201205008R .

Radić, Ivan, Mirić, Mirjana, Mijović, Milica, Tatalović, Nikola, Mitić, Miloš, Nestorović, Vojkan, Adžić, Miroslav, Blagojević, Duško, Popović, Ljiljana, Janićijević-Hudomal, Snežana, "Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption" in Archives of Biological Sciences, 73, no. 1 (2021):123-133,
https://doi.org/10.2298/ABS201205008R . .

TY  - JOUR
AU  - Radić, Ivan
AU  - Mijović, Milica
AU  - Tatalović, Nikola R.
AU  - Mitić, Miloš
AU  - Lukić, Vera
AU  - Joksimović, Bojan
AU  - Petrović, Zorica
AU  - Ristić, Siniša
AU  - Veličković, Stefan
AU  - Nestorović, Vojkan
AU  - Ćorac, Aleksandar M.
AU  - Mirić, Mirjana
AU  - Adžić, Miroslav
AU  - Blagojević, Duško P.
AU  - Popović, Ljiljana M.
AU  - Janićijević-Hudomal, Snežana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8541
AB  - In 2012, alcohol liver disease resulted in 3.3 million—5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey (per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper–zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol ingestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling. © The Author(s) 2019.
T2  - Human and Experimental Toxicology
T1  - Protective effects of whey on rat liver damage induced by chronic alcohol intake
VL  - 38
IS  - 6
SP  - 632
EP  - 645
DO  - 10.1177/0960327119829518
ER  - 

@article{
author = "Radić, Ivan and Mijović, Milica and Tatalović, Nikola R. and Mitić, Miloš and Lukić, Vera and Joksimović, Bojan and Petrović, Zorica and Ristić, Siniša and Veličković, Stefan and Nestorović, Vojkan and Ćorac, Aleksandar M. and Mirić, Mirjana and Adžić, Miroslav and Blagojević, Duško P. and Popović, Ljiljana M. and Janićijević-Hudomal, Snežana",
year = "2019",
abstract = "In 2012, alcohol liver disease resulted in 3.3 million—5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey (per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper–zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol ingestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling. © The Author(s) 2019.",
journal = "Human and Experimental Toxicology",
title = "Protective effects of whey on rat liver damage induced by chronic alcohol intake",
volume = "38",
number = "6",
pages = "632-645",
doi = "10.1177/0960327119829518"
}

Radić, I., Mijović, M., Tatalović, N. R., Mitić, M., Lukić, V., Joksimović, B., Petrović, Z., Ristić, S., Veličković, S., Nestorović, V., Ćorac, A. M., Mirić, M., Adžić, M., Blagojević, D. P., Popović, L. M.,& Janićijević-Hudomal, S.. (2019). Protective effects of whey on rat liver damage induced by chronic alcohol intake. in Human and Experimental Toxicology, 38(6), 632-645.
https://doi.org/10.1177/0960327119829518

Radić I, Mijović M, Tatalović NR, Mitić M, Lukić V, Joksimović B, Petrović Z, Ristić S, Veličković S, Nestorović V, Ćorac AM, Mirić M, Adžić M, Blagojević DP, Popović LM, Janićijević-Hudomal S. Protective effects of whey on rat liver damage induced by chronic alcohol intake. in Human and Experimental Toxicology. 2019;38(6):632-645.
doi:10.1177/0960327119829518 .

Radić, Ivan, Mijović, Milica, Tatalović, Nikola R., Mitić, Miloš, Lukić, Vera, Joksimović, Bojan, Petrović, Zorica, Ristić, Siniša, Veličković, Stefan, Nestorović, Vojkan, Ćorac, Aleksandar M., Mirić, Mirjana, Adžić, Miroslav, Blagojević, Duško P., Popović, Ljiljana M., Janićijević-Hudomal, Snežana, "Protective effects of whey on rat liver damage induced by chronic alcohol intake" in Human and Experimental Toxicology, 38, no. 6 (2019):632-645,
https://doi.org/10.1177/0960327119829518 . .

TY  - JOUR
AU  - Radić, Ivan
AU  - Nestorović, Vojkan
AU  - Mijović, Milica
AU  - Tatalović, Nikola R.
AU  - Joksimović, Bojan
AU  - Lukić, Vera
AU  - Mitić, Miloš
AU  - Adžić, Miroslav
AU  - Blagojević, Duško P.
AU  - Veličković, Stefan
AU  - Bulajić, Sonja
AU  - Đerković, Branislav
AU  - Mirić, Mirjana
AU  - Janićijević-Hudomal, Snežana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7931
AB  - We studied the effects of whey and pumpkin seed oil supplementation on the biochemical parameters in blood serum of male rats after chronic ad libitum alcohol consumption. The levels of AST, ALT, total bilirubin, ALP, LDH, triglycerides, total cholesterol, HDL, LDL, VLDL, triglyceride/HDL ratio, total cholesterol/HDL ratio (cholesterol ratio) and LDL/HDL ratio (index of atherosclerosis) were determined in rats after six weeks of treatment with: (i) ethanol (12% ethanol, ad libitum), (ii) whey (2 g/kg per day), (iii) pumpkin seed oil (2 mL/kg per day), (iv) both ethanol and whey, and (v) both ethanol and pumpkin seed oil. The results showed no changes in the levels of AST, ALT, total bilirubin, ALP, total cholesterol, HDL and VLDL in alcoholic rats when compared to the controls (fed with a standard laboratory diet ad libitum) and rats supplemented with whey and pumpkin seed oil. Our results suggest that alcohol consumption in small doses for 6 weeks changes lipid metabolism and significantly elevates the LDL/HDL ratio (index of atherosclerosis) but does not induce extensive liver damage. Ethanol consumption in our experimental conditions lowered the triglyceride level as well as the triglyceride/HDL ratio, suggesting lipid redistribution and the induction of some cardio-protective effect. However, ethanol induced a higher index of atherosclerosis. Pumpkin seed oil showed some protective potential in alcoholic rats by lowering the total cholesterol/HDL ratio, but it elevated the LDH. Whey consumption prevented elevation of the atherosclerosis index, pointing to its protective role, probably through the redistribution of lipids. However, whey in combination with ethanol elevated LDH.
T2  - Archives of Biological Sciences
T1  - The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol
VL  - 70
IS  - 3
SP  - 531
EP  - 541
DO  - 10.2298/ABS180320014R
ER  - 

@article{
author = "Radić, Ivan and Nestorović, Vojkan and Mijović, Milica and Tatalović, Nikola R. and Joksimović, Bojan and Lukić, Vera and Mitić, Miloš and Adžić, Miroslav and Blagojević, Duško P. and Veličković, Stefan and Bulajić, Sonja and Đerković, Branislav and Mirić, Mirjana and Janićijević-Hudomal, Snežana",
year = "2018",
abstract = "We studied the effects of whey and pumpkin seed oil supplementation on the biochemical parameters in blood serum of male rats after chronic ad libitum alcohol consumption. The levels of AST, ALT, total bilirubin, ALP, LDH, triglycerides, total cholesterol, HDL, LDL, VLDL, triglyceride/HDL ratio, total cholesterol/HDL ratio (cholesterol ratio) and LDL/HDL ratio (index of atherosclerosis) were determined in rats after six weeks of treatment with: (i) ethanol (12% ethanol, ad libitum), (ii) whey (2 g/kg per day), (iii) pumpkin seed oil (2 mL/kg per day), (iv) both ethanol and whey, and (v) both ethanol and pumpkin seed oil. The results showed no changes in the levels of AST, ALT, total bilirubin, ALP, total cholesterol, HDL and VLDL in alcoholic rats when compared to the controls (fed with a standard laboratory diet ad libitum) and rats supplemented with whey and pumpkin seed oil. Our results suggest that alcohol consumption in small doses for 6 weeks changes lipid metabolism and significantly elevates the LDL/HDL ratio (index of atherosclerosis) but does not induce extensive liver damage. Ethanol consumption in our experimental conditions lowered the triglyceride level as well as the triglyceride/HDL ratio, suggesting lipid redistribution and the induction of some cardio-protective effect. However, ethanol induced a higher index of atherosclerosis. Pumpkin seed oil showed some protective potential in alcoholic rats by lowering the total cholesterol/HDL ratio, but it elevated the LDH. Whey consumption prevented elevation of the atherosclerosis index, pointing to its protective role, probably through the redistribution of lipids. However, whey in combination with ethanol elevated LDH.",
journal = "Archives of Biological Sciences",
title = "The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol",
volume = "70",
number = "3",
pages = "531-541",
doi = "10.2298/ABS180320014R"
}

Radić, I., Nestorović, V., Mijović, M., Tatalović, N. R., Joksimović, B., Lukić, V., Mitić, M., Adžić, M., Blagojević, D. P., Veličković, S., Bulajić, S., Đerković, B., Mirić, M.,& Janićijević-Hudomal, S.. (2018). The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol. in Archives of Biological Sciences, 70(3), 531-541.
https://doi.org/10.2298/ABS180320014R

Radić I, Nestorović V, Mijović M, Tatalović NR, Joksimović B, Lukić V, Mitić M, Adžić M, Blagojević DP, Veličković S, Bulajić S, Đerković B, Mirić M, Janićijević-Hudomal S. The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol. in Archives of Biological Sciences. 2018;70(3):531-541.
doi:10.2298/ABS180320014R .

Radić, Ivan, Nestorović, Vojkan, Mijović, Milica, Tatalović, Nikola R., Joksimović, Bojan, Lukić, Vera, Mitić, Miloš, Adžić, Miroslav, Blagojević, Duško P., Veličković, Stefan, Bulajić, Sonja, Đerković, Branislav, Mirić, Mirjana, Janićijević-Hudomal, Snežana, "The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol" in Archives of Biological Sciences, 70, no. 3 (2018):531-541,
https://doi.org/10.2298/ABS180320014R . .