TY  - JOUR
AU  - Milin-Lazović, Jelena
AU  - Madžarević, Petar
AU  - Rajović, Nina
AU  - Đorđević, Vladimir
AU  - Milić, Nikola
AU  - Pavlović, Sonja
AU  - Veljković, Nevena V.
AU  - Milić, Nataša M.
AU  - Radenković, Dejan
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9861
AB  - Introduction: The analysis of cell-free DNA (cfDNA) for genetic abnormalities is a promising new approach for the diagnosis and prognosis of pancreatic cancer patients. Insights into the molecular characteristics of pancreatic cancer may provide valuable information, leading to its earlier detection and the development of targeted therapies. Material and Methods: We conducted a systematic review and a meta-analysis of studies that reported cfDNA in pancreatic ductal adenocarcinoma (PDAC). The studies were considered eligible if they included patients with PDAC, if they had blood tests for cfDNA/ctDNA, and if they analyzed the prognostic value of cfDNA/ctDNA for patients’ survival. The studies published before 22 October 2020 were identified through the PubMED, EMBASE, Web of Science and Cochrane Library databases. The assessed outcomes were the overall (OS) and progression-free survival (PFS), expressed as the log hazard ratio (HR) and standard error (SE). The summary of the HR effect size was estimated by pooling the individual trial results using the Review Manager, version 5.3, Cochrane Collaboration. The heterogeneity was assessed using the Cochran Q test and I2 statistic. Results: In total, 48 studies were included in the qualitative review, while 44 were assessed in the quantitative synthesis, with the total number of patients included being 3524. Overall negative impacts of cfDNA and KRAS mutations on OS and PFS in PDAC (HR = 2.42, 95% CI: 1.95–2.99 and HR = 2.46, 95% CI: 2.01–3.00, respectively) were found. The subgroup analysis of the locally advanced and metastatic disease presented similar results (HR = 2.51, 95% CI: 1.90–3.31). In the studies assessing the pre-treatment presence of KRAS, there was a moderate to high degree of heterogeneity (I2 = 87% and I2 = 48%, for OS and PFS, respectively), which was remarkably decreased in the analysis of the studies measuring post-treatment KRAS (I2 = 24% and I2 = 0%, for OS and PFS, respectively). The patients who were KRAS positive before but KRAS negative after treatment had a better prognosis than the persistently KRAS-positive patients (HR = 5.30, 95% CI: 1.02–27.63). Conclusion: The assessment of KRAS mutation by liquid biopsy can be considered as an additional tool for the estimation of the disease course and outcome in PDAC patients.
T2  - Cancers
T1  - Meta-analysis of circulating cell-free dna’s role in the prognosis of pancreatic cancer
VL  - 13
IS  - 14
SP  - 3378
DO  - 10.3390/cancers13143378
ER  - 

@article{
author = "Milin-Lazović, Jelena and Madžarević, Petar and Rajović, Nina and Đorđević, Vladimir and Milić, Nikola and Pavlović, Sonja and Veljković, Nevena V. and Milić, Nataša M. and Radenković, Dejan",
year = "2021",
abstract = "Introduction: The analysis of cell-free DNA (cfDNA) for genetic abnormalities is a promising new approach for the diagnosis and prognosis of pancreatic cancer patients. Insights into the molecular characteristics of pancreatic cancer may provide valuable information, leading to its earlier detection and the development of targeted therapies. Material and Methods: We conducted a systematic review and a meta-analysis of studies that reported cfDNA in pancreatic ductal adenocarcinoma (PDAC). The studies were considered eligible if they included patients with PDAC, if they had blood tests for cfDNA/ctDNA, and if they analyzed the prognostic value of cfDNA/ctDNA for patients’ survival. The studies published before 22 October 2020 were identified through the PubMED, EMBASE, Web of Science and Cochrane Library databases. The assessed outcomes were the overall (OS) and progression-free survival (PFS), expressed as the log hazard ratio (HR) and standard error (SE). The summary of the HR effect size was estimated by pooling the individual trial results using the Review Manager, version 5.3, Cochrane Collaboration. The heterogeneity was assessed using the Cochran Q test and I2 statistic. Results: In total, 48 studies were included in the qualitative review, while 44 were assessed in the quantitative synthesis, with the total number of patients included being 3524. Overall negative impacts of cfDNA and KRAS mutations on OS and PFS in PDAC (HR = 2.42, 95% CI: 1.95–2.99 and HR = 2.46, 95% CI: 2.01–3.00, respectively) were found. The subgroup analysis of the locally advanced and metastatic disease presented similar results (HR = 2.51, 95% CI: 1.90–3.31). In the studies assessing the pre-treatment presence of KRAS, there was a moderate to high degree of heterogeneity (I2 = 87% and I2 = 48%, for OS and PFS, respectively), which was remarkably decreased in the analysis of the studies measuring post-treatment KRAS (I2 = 24% and I2 = 0%, for OS and PFS, respectively). The patients who were KRAS positive before but KRAS negative after treatment had a better prognosis than the persistently KRAS-positive patients (HR = 5.30, 95% CI: 1.02–27.63). Conclusion: The assessment of KRAS mutation by liquid biopsy can be considered as an additional tool for the estimation of the disease course and outcome in PDAC patients.",
journal = "Cancers",
title = "Meta-analysis of circulating cell-free dna’s role in the prognosis of pancreatic cancer",
volume = "13",
number = "14",
pages = "3378",
doi = "10.3390/cancers13143378"
}

Milin-Lazović, J., Madžarević, P., Rajović, N., Đorđević, V., Milić, N., Pavlović, S., Veljković, N. V., Milić, N. M.,& Radenković, D.. (2021). Meta-analysis of circulating cell-free dna’s role in the prognosis of pancreatic cancer. in Cancers, 13(14), 3378.
https://doi.org/10.3390/cancers13143378

Milin-Lazović J, Madžarević P, Rajović N, Đorđević V, Milić N, Pavlović S, Veljković NV, Milić NM, Radenković D. Meta-analysis of circulating cell-free dna’s role in the prognosis of pancreatic cancer. in Cancers. 2021;13(14):3378.
doi:10.3390/cancers13143378 .

Milin-Lazović, Jelena, Madžarević, Petar, Rajović, Nina, Đorđević, Vladimir, Milić, Nikola, Pavlović, Sonja, Veljković, Nevena V., Milić, Nataša M., Radenković, Dejan, "Meta-analysis of circulating cell-free dna’s role in the prognosis of pancreatic cancer" in Cancers, 13, no. 14 (2021):3378,
https://doi.org/10.3390/cancers13143378 . .

TY  - JOUR
AU  - Drljača, Tamara
AU  - Zukić, Branka
AU  - Kovačević, Vladimir
AU  - Gemović, Branislava S.
AU  - Karan-Đurašević, Teodora
AU  - Perović, Vladimir R.
AU  - Lazarević, Mladen
AU  - Pavlović, Sonja
AU  - Veljković, Nevena V.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10769
AB  - The complete understanding of the genomic contribution to complex traits, diseases, and response to treatments, as well as genomic medicine application to the well-being of all humans will be achieved through the global variome that encompasses fine-scale genetic diversity. Despite significant efforts in recent years, uneven representation still characterizes genomic resources and among the underrepresented European populations are the Western Balkans including the Serbian population. Our research addresses this gap and presents the first ever targeted sequencing dataset of variants in clinically relevant genes. By measuring population differentiation and applying the Principal Component and Admixture analysis we demonstrated that the Serbian population differs little from other European populations, yet we identified several novel and more frequent variants that appear as its unique genetic determinants. We explored thoroughly the functional impact of frequent variants and its correlation with the health burden of the population of Serbia based on a sample of 144 individuals. Our variants catalogue improves the understanding of genetics of modern Serbia, contributes to research on ancestry, and aids in improvements of well-being and health equity. In addition, this resource may also be applicable in neighboring regions and valuable in worldwide functional analyses of genetic variants in individuals of European descent.
T2  - Scientific Reports
T1  - The first insight into the genetic structure of the population of modern Serbia
VL  - 11
IS  - 1
SP  - 13995
DO  - 10.1038/s41598-021-93129-4
ER  - 

@article{
author = "Drljača, Tamara and Zukić, Branka and Kovačević, Vladimir and Gemović, Branislava S. and Karan-Đurašević, Teodora and Perović, Vladimir R. and Lazarević, Mladen and Pavlović, Sonja and Veljković, Nevena V.",
year = "2021",
abstract = "The complete understanding of the genomic contribution to complex traits, diseases, and response to treatments, as well as genomic medicine application to the well-being of all humans will be achieved through the global variome that encompasses fine-scale genetic diversity. Despite significant efforts in recent years, uneven representation still characterizes genomic resources and among the underrepresented European populations are the Western Balkans including the Serbian population. Our research addresses this gap and presents the first ever targeted sequencing dataset of variants in clinically relevant genes. By measuring population differentiation and applying the Principal Component and Admixture analysis we demonstrated that the Serbian population differs little from other European populations, yet we identified several novel and more frequent variants that appear as its unique genetic determinants. We explored thoroughly the functional impact of frequent variants and its correlation with the health burden of the population of Serbia based on a sample of 144 individuals. Our variants catalogue improves the understanding of genetics of modern Serbia, contributes to research on ancestry, and aids in improvements of well-being and health equity. In addition, this resource may also be applicable in neighboring regions and valuable in worldwide functional analyses of genetic variants in individuals of European descent.",
journal = "Scientific Reports",
title = "The first insight into the genetic structure of the population of modern Serbia",
volume = "11",
number = "1",
pages = "13995",
doi = "10.1038/s41598-021-93129-4"
}

Drljača, T., Zukić, B., Kovačević, V., Gemović, B. S., Karan-Đurašević, T., Perović, V. R., Lazarević, M., Pavlović, S.,& Veljković, N. V.. (2021). The first insight into the genetic structure of the population of modern Serbia. in Scientific Reports, 11(1), 13995.
https://doi.org/10.1038/s41598-021-93129-4

Drljača T, Zukić B, Kovačević V, Gemović BS, Karan-Đurašević T, Perović VR, Lazarević M, Pavlović S, Veljković NV. The first insight into the genetic structure of the population of modern Serbia. in Scientific Reports. 2021;11(1):13995.
doi:10.1038/s41598-021-93129-4 .

Drljača, Tamara, Zukić, Branka, Kovačević, Vladimir, Gemović, Branislava S., Karan-Đurašević, Teodora, Perović, Vladimir R., Lazarević, Mladen, Pavlović, Sonja, Veljković, Nevena V., "The first insight into the genetic structure of the population of modern Serbia" in Scientific Reports, 11, no. 1 (2021):13995,
https://doi.org/10.1038/s41598-021-93129-4 . .

TY  - JOUR
AU  - Mihaljević, Marina
AU  - Franić, Dušanka
AU  - Soldatović, Ivan A.
AU  - Lukić, Iva
AU  - Andrić-Petrović, Sanja
AU  - Mirjanić, Tijana
AU  - Stanković, Biljana
AU  - Žukić, Branka
AU  - Željić, Katarina
AU  - Gašić, Vladimir
AU  - Novaković, Ivana
AU  - Pavlović, Sonja
AU  - Adžić, Miroslav
AU  - Marić, Nađa P.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9748
AB  - Hypothalamic–pituitary–adrenal (HPA) axis activity mediates the relationship between childhood trauma (CT) and psychosis. The FKBP5 gene, one of the key regulators of HPA axis activity after stress exposure, has been found associated with psychosis. Allele-specific and CT related FKBP5 demethylation in intron 7 was revealed in different psychiatric disorders. However, no studies have investigated FKBP5 methylation in subjects with different genetic liability for psychosis. A total of 144 participants were included in the study: 48 patients with psychotic disorders, 50 unaffected siblings, and 46 healthy controls. CT was assessed by Childhood Trauma Questionnaire. The FKBP5 rs1360780 was genotyped and FKBP5 methylation analyses were performed using bisulfite conversion followed by Sanger sequencing at three CpG sites in intron 7. Mixed linear model was used to assess group differences depending on rs1360780 T allele and CT. Results showed a significant T allele-dependent decrease of FKBP5 methylation in patients compared to unaffected siblings and controls. Effect of interaction between T allele and CT exposure on FKBP5 demethylation was found in controls. No effect of both risk factors (T allele and CT) on FKBP5 methylation level was found in unaffected siblings. We confirmed previous evidence of the association between the FKBP5 rs1360780 T allele, CT, and decreased FKBP5 methylation in intron 7. Allele-specific FKBP5 demethylation found in patients could shed a light on altered HPA axis activity in a subgroup of patients related to stress-induced psychosis. FKBP5 methylation and potential protective mechanisms in unaffected siblings after trauma exposure require further investigation. © 2021 Elsevier Ltd
T2  - Psychoneuroendocrinology
T1  - The FKBP5 genotype and childhood trauma effects on FKBP5 DNA methylation in patients with psychosis, their unaffected siblings, and healthy controls
VL  - 128
SP  - 105205
DO  - 10.1016/j.psyneuen.2021.105205
ER  - 

@article{
author = "Mihaljević, Marina and Franić, Dušanka and Soldatović, Ivan A. and Lukić, Iva and Andrić-Petrović, Sanja and Mirjanić, Tijana and Stanković, Biljana and Žukić, Branka and Željić, Katarina and Gašić, Vladimir and Novaković, Ivana and Pavlović, Sonja and Adžić, Miroslav and Marić, Nađa P.",
year = "2021",
abstract = "Hypothalamic–pituitary–adrenal (HPA) axis activity mediates the relationship between childhood trauma (CT) and psychosis. The FKBP5 gene, one of the key regulators of HPA axis activity after stress exposure, has been found associated with psychosis. Allele-specific and CT related FKBP5 demethylation in intron 7 was revealed in different psychiatric disorders. However, no studies have investigated FKBP5 methylation in subjects with different genetic liability for psychosis. A total of 144 participants were included in the study: 48 patients with psychotic disorders, 50 unaffected siblings, and 46 healthy controls. CT was assessed by Childhood Trauma Questionnaire. The FKBP5 rs1360780 was genotyped and FKBP5 methylation analyses were performed using bisulfite conversion followed by Sanger sequencing at three CpG sites in intron 7. Mixed linear model was used to assess group differences depending on rs1360780 T allele and CT. Results showed a significant T allele-dependent decrease of FKBP5 methylation in patients compared to unaffected siblings and controls. Effect of interaction between T allele and CT exposure on FKBP5 demethylation was found in controls. No effect of both risk factors (T allele and CT) on FKBP5 methylation level was found in unaffected siblings. We confirmed previous evidence of the association between the FKBP5 rs1360780 T allele, CT, and decreased FKBP5 methylation in intron 7. Allele-specific FKBP5 demethylation found in patients could shed a light on altered HPA axis activity in a subgroup of patients related to stress-induced psychosis. FKBP5 methylation and potential protective mechanisms in unaffected siblings after trauma exposure require further investigation. © 2021 Elsevier Ltd",
journal = "Psychoneuroendocrinology",
title = "The FKBP5 genotype and childhood trauma effects on FKBP5 DNA methylation in patients with psychosis, their unaffected siblings, and healthy controls",
volume = "128",
pages = "105205",
doi = "10.1016/j.psyneuen.2021.105205"
}

Mihaljević, M., Franić, D., Soldatović, I. A., Lukić, I., Andrić-Petrović, S., Mirjanić, T., Stanković, B., Žukić, B., Željić, K., Gašić, V., Novaković, I., Pavlović, S., Adžić, M.,& Marić, N. P.. (2021). The FKBP5 genotype and childhood trauma effects on FKBP5 DNA methylation in patients with psychosis, their unaffected siblings, and healthy controls. in Psychoneuroendocrinology, 128, 105205.
https://doi.org/10.1016/j.psyneuen.2021.105205

Mihaljević M, Franić D, Soldatović IA, Lukić I, Andrić-Petrović S, Mirjanić T, Stanković B, Žukić B, Željić K, Gašić V, Novaković I, Pavlović S, Adžić M, Marić NP. The FKBP5 genotype and childhood trauma effects on FKBP5 DNA methylation in patients with psychosis, their unaffected siblings, and healthy controls. in Psychoneuroendocrinology. 2021;128:105205.
doi:10.1016/j.psyneuen.2021.105205 .

Mihaljević, Marina, Franić, Dušanka, Soldatović, Ivan A., Lukić, Iva, Andrić-Petrović, Sanja, Mirjanić, Tijana, Stanković, Biljana, Žukić, Branka, Željić, Katarina, Gašić, Vladimir, Novaković, Ivana, Pavlović, Sonja, Adžić, Miroslav, Marić, Nađa P., "The FKBP5 genotype and childhood trauma effects on FKBP5 DNA methylation in patients with psychosis, their unaffected siblings, and healthy controls" in Psychoneuroendocrinology, 128 (2021):105205,
https://doi.org/10.1016/j.psyneuen.2021.105205 . .

TY  - CONF
AU  - Marić, Nađa
AU  - Mihaljević, Marina
AU  - Franić, Dušanka
AU  - Soldatovic, Ivan
AU  - Andrić, Sanja
AU  - Lukić, Iva
AU  - Mirjanic, Tijana
AU  - Stankovic, Biljana
AU  - Zukic, Branka
AU  - Dobricic, Valerija
AU  - Novaković, Ivana
AU  - Pavlović, Sonja
AU  - Adžić, Miroslav
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7156
C3  - Schizophrenia Bulletin
T1  - The Signature of Trauma in Psychosis: a Preliminary Genetic and Epigenetic Analyses of Fk506-Binding Protein 5 Regulation
VL  - 43
SP  - S66
EP  - S66
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7156
ER  - 

@conference{
author = "Marić, Nađa and Mihaljević, Marina and Franić, Dušanka and Soldatovic, Ivan and Andrić, Sanja and Lukić, Iva and Mirjanic, Tijana and Stankovic, Biljana and Zukic, Branka and Dobricic, Valerija and Novaković, Ivana and Pavlović, Sonja and Adžić, Miroslav",
year = "2017",
journal = "Schizophrenia Bulletin",
title = "The Signature of Trauma in Psychosis: a Preliminary Genetic and Epigenetic Analyses of Fk506-Binding Protein 5 Regulation",
volume = "43",
pages = "S66-S66",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7156"
}

Marić, N., Mihaljević, M., Franić, D., Soldatovic, I., Andrić, S., Lukić, I., Mirjanic, T., Stankovic, B., Zukic, B., Dobricic, V., Novaković, I., Pavlović, S.,& Adžić, M.. (2017). The Signature of Trauma in Psychosis: a Preliminary Genetic and Epigenetic Analyses of Fk506-Binding Protein 5 Regulation. in Schizophrenia Bulletin, 43, S66-S66.
https://hdl.handle.net/21.15107/rcub_vinar_7156

Marić N, Mihaljević M, Franić D, Soldatovic I, Andrić S, Lukić I, Mirjanic T, Stankovic B, Zukic B, Dobricic V, Novaković I, Pavlović S, Adžić M. The Signature of Trauma in Psychosis: a Preliminary Genetic and Epigenetic Analyses of Fk506-Binding Protein 5 Regulation. in Schizophrenia Bulletin. 2017;43:S66-S66.
https://hdl.handle.net/21.15107/rcub_vinar_7156 .

Marić, Nađa, Mihaljević, Marina, Franić, Dušanka, Soldatovic, Ivan, Andrić, Sanja, Lukić, Iva, Mirjanic, Tijana, Stankovic, Biljana, Zukic, Branka, Dobricic, Valerija, Novaković, Ivana, Pavlović, Sonja, Adžić, Miroslav, "The Signature of Trauma in Psychosis: a Preliminary Genetic and Epigenetic Analyses of Fk506-Binding Protein 5 Regulation" in Schizophrenia Bulletin, 43 (2017):S66-S66,
https://hdl.handle.net/21.15107/rcub_vinar_7156 .