TY  - JOUR
AU  - Đikić, Dragoslava
AU  - Budec, Mirela
AU  - Vranješ-Đurić, Sanja
AU  - Todorović, Vera
AU  - Drndarević, Neda C.
AU  - Vignjevic, Sanja
AU  - Mitrovic, Olivera
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5130
AB  - Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect of ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor.
T2  - Pharmacological Reports
T1  - Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex
VL  - 64
IS  - 4
SP  - 896
EP  - 901
DO  - 10.1016/S1734-1140(12)70884-8
ER  - 

@article{
author = "Đikić, Dragoslava and Budec, Mirela and Vranješ-Đurić, Sanja and Todorović, Vera and Drndarević, Neda C. and Vignjevic, Sanja and Mitrovic, Olivera",
year = "2012",
abstract = "Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect of ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor.",
journal = "Pharmacological Reports",
title = "Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex",
volume = "64",
number = "4",
pages = "896-901",
doi = "10.1016/S1734-1140(12)70884-8"
}

Đikić, D., Budec, M., Vranješ-Đurić, S., Todorović, V., Drndarević, N. C., Vignjevic, S.,& Mitrovic, O.. (2012). Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex. in Pharmacological Reports, 64(4), 896-901.
https://doi.org/10.1016/S1734-1140(12)70884-8

Đikić D, Budec M, Vranješ-Đurić S, Todorović V, Drndarević NC, Vignjevic S, Mitrovic O. Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex. in Pharmacological Reports. 2012;64(4):896-901.
doi:10.1016/S1734-1140(12)70884-8 .

Đikić, Dragoslava, Budec, Mirela, Vranješ-Đurić, Sanja, Todorović, Vera, Drndarević, Neda C., Vignjevic, Sanja, Mitrovic, Olivera, "Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex" in Pharmacological Reports, 64, no. 4 (2012):896-901,
https://doi.org/10.1016/S1734-1140(12)70884-8 . .

TY  - JOUR
AU  - Milićević, Zorka T.
AU  - Petković, Marijana
AU  - Drndarević, Neda C.
AU  - Pavlović, Mirjana D.
AU  - Todorović, Vera N.
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3188
AB  - The role of heat shock protein 70 (HSP70) expression has been investigated in various types of tumors. There are only little and controversial data about its clinical relevance in colorectal carcinoma, one of the most common carcinomas observed in humans. In this study we investigated expression of HSP70 in human colonic carcinoma and possible correlation with clinicopathology. To assess patterns (cytosolic and membrane) of HSP70 expression, the 48 surgically removed colorectal adenocarcinomas and 12 normal colonic and rectal mucosal samples were examined by immunohistochemistry and Western-blot. According to results of immunohistochemistry, expression of cytoplasmic HSP72 was significantly higher in colorectal carcinoma compared with normal and adjacent mucosa (p LT 0.01). In addition, there was significant increase in HSP72 expression in lymph node-positive compared to node-nevative group (p LT 0.001). Dukes C2 stage of colonic cancer showed significantly higher immunohistochemical score than Dukes B2 and B1 stage groups (p LT 0.05 i.e. p LT 0.02). There was no relation between expression of HSP72 and degree of tumor differentiation. Using Western blot analyses, we noticed elevated levels of cytosolic HSP70 in colorectal cancer cells compared to normal. Densitometric analysis of blots of plasma membrane HSP70 expression has shown decrease in colorectal cancer cells compared to normal mucosa. According to our results, overexpression of HSP72 in malignant tissues of patients with colorectal carcinoma is related to tumor progression, suggesting that these proteins could play an important role not only in tumorigenesis but also in the development of drug resistance. Further research is necessary to clarify the mechanisms responsible for differential HSP70 expression as well as its definitive role in colorectal cancer.
T2  - Neoplasma
T1  - Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis
VL  - 54
IS  - 1
SP  - 37
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3188
ER  - 

@article{
author = "Milićević, Zorka T. and Petković, Marijana and Drndarević, Neda C. and Pavlović, Mirjana D. and Todorović, Vera N.",
year = "2007",
abstract = "The role of heat shock protein 70 (HSP70) expression has been investigated in various types of tumors. There are only little and controversial data about its clinical relevance in colorectal carcinoma, one of the most common carcinomas observed in humans. In this study we investigated expression of HSP70 in human colonic carcinoma and possible correlation with clinicopathology. To assess patterns (cytosolic and membrane) of HSP70 expression, the 48 surgically removed colorectal adenocarcinomas and 12 normal colonic and rectal mucosal samples were examined by immunohistochemistry and Western-blot. According to results of immunohistochemistry, expression of cytoplasmic HSP72 was significantly higher in colorectal carcinoma compared with normal and adjacent mucosa (p LT 0.01). In addition, there was significant increase in HSP72 expression in lymph node-positive compared to node-nevative group (p LT 0.001). Dukes C2 stage of colonic cancer showed significantly higher immunohistochemical score than Dukes B2 and B1 stage groups (p LT 0.05 i.e. p LT 0.02). There was no relation between expression of HSP72 and degree of tumor differentiation. Using Western blot analyses, we noticed elevated levels of cytosolic HSP70 in colorectal cancer cells compared to normal. Densitometric analysis of blots of plasma membrane HSP70 expression has shown decrease in colorectal cancer cells compared to normal mucosa. According to our results, overexpression of HSP72 in malignant tissues of patients with colorectal carcinoma is related to tumor progression, suggesting that these proteins could play an important role not only in tumorigenesis but also in the development of drug resistance. Further research is necessary to clarify the mechanisms responsible for differential HSP70 expression as well as its definitive role in colorectal cancer.",
journal = "Neoplasma",
title = "Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis",
volume = "54",
number = "1",
pages = "37-45",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3188"
}

Milićević, Z. T., Petković, M., Drndarević, N. C., Pavlović, M. D.,& Todorović, V. N.. (2007). Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis. in Neoplasma, 54(1), 37-45.
https://hdl.handle.net/21.15107/rcub_vinar_3188

Milićević ZT, Petković M, Drndarević NC, Pavlović MD, Todorović VN. Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis. in Neoplasma. 2007;54(1):37-45.
https://hdl.handle.net/21.15107/rcub_vinar_3188 .

Milićević, Zorka T., Petković, Marijana, Drndarević, Neda C., Pavlović, Mirjana D., Todorović, Vera N., "Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis" in Neoplasma, 54, no. 1 (2007):37-45,
https://hdl.handle.net/21.15107/rcub_vinar_3188 .