TY  - JOUR
AU  - Milanović, Zorana
AU  - Ilić Božović, Anja
AU  - Bojić-Trbojević, Žanka
AU  - Hajduković, Ljiljana
AU  - Francuski Andrić, Jelena
AU  - Kovačević Filipović, Milica
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12998
AB  - Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causinganemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of theerythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parametersfocusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days aftertreatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolicand inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified timepoints. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I wasassessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravitalstaining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference.Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocytecount was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs,although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decreasein ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phaseprotein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.
T2  - Macedonian Veterinary Review
T1  - Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs
VL  - 47
IS  - 1
SP  - i
EP  - ix
DO  - 10.2478/macvetrev-2024-0011
ER  - 

@article{
author = "Milanović, Zorana and Ilić Božović, Anja and Bojić-Trbojević, Žanka and Hajduković, Ljiljana and Francuski Andrić, Jelena and Kovačević Filipović, Milica",
year = "2024",
abstract = "Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causinganemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of theerythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parametersfocusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days aftertreatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolicand inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified timepoints. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I wasassessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravitalstaining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference.Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocytecount was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs,although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decreasein ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phaseprotein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.",
journal = "Macedonian Veterinary Review",
title = "Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs",
volume = "47",
number = "1",
pages = "i-ix",
doi = "10.2478/macvetrev-2024-0011"
}

Milanović, Z., Ilić Božović, A., Bojić-Trbojević, Ž., Hajduković, L., Francuski Andrić, J.,& Kovačević Filipović, M.. (2024). Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs. in Macedonian Veterinary Review, 47(1), i-ix.
https://doi.org/10.2478/macvetrev-2024-0011

Milanović Z, Ilić Božović A, Bojić-Trbojević Ž, Hajduković L, Francuski Andrić J, Kovačević Filipović M. Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs. in Macedonian Veterinary Review. 2024;47(1):i-ix.
doi:10.2478/macvetrev-2024-0011 .

Milanović, Zorana, Ilić Božović, Anja, Bojić-Trbojević, Žanka, Hajduković, Ljiljana, Francuski Andrić, Jelena, Kovačević Filipović, Milica, "Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs" in Macedonian Veterinary Review, 47, no. 1 (2024):i-ix,
https://doi.org/10.2478/macvetrev-2024-0011 . .

TY  - JOUR
AU  - Van Gool, Alain
AU  - Corrales, Fernado
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Oliver-Martos, Begona
AU  - Martínez-Cáceres, Eva
AU  - Jakasa, Ivone
AU  - Gajski, Goran
AU  - Brun, Virginie
AU  - Kyriacou, Kyriacos
AU  - Burzynska-Pedziwiatr, Izabela
AU  - Wozniak, Lucyna Alicja
AU  - Nierkens, Stephan
AU  - Pascual García, César
AU  - Katrlik, Jaroslav
AU  - Bojić-Trbojević, Žanka
AU  - Vacek, Jan
AU  - Llorente, Alicia
AU  - Antohe, Felicia
AU  - Suica, Viorel
AU  - Suarez, Guillaume
AU  - T’Kindt, Ruben
AU  - Martin, Petra
AU  - Penque, Deborah
AU  - Martins, Ines Lanca
AU  - Bodoki, Ede
AU  - Iacob, Bogdan-Cezar
AU  - Aydindogan, Eda
AU  - Timur, Suna
AU  - Allinson, John
AU  - Sutton, Christopher
AU  - Luider, Theo
AU  - Wittfooth, Saara
AU  - Sammar, Marei
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9018
AB  - Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.
T2  - Expert Review of Proteomics
T1  - Analytical techniques for multiplex analysis of protein biomarkers
VL  - 17
IS  - 4
SP  - 257
EP  - 273
DO  - 10.1080/14789450.2020.1763174
ER  - 

@article{
author = "Van Gool, Alain and Corrales, Fernado and Čolović, Mirjana B. and Krstić, Danijela Z. and Oliver-Martos, Begona and Martínez-Cáceres, Eva and Jakasa, Ivone and Gajski, Goran and Brun, Virginie and Kyriacou, Kyriacos and Burzynska-Pedziwiatr, Izabela and Wozniak, Lucyna Alicja and Nierkens, Stephan and Pascual García, César and Katrlik, Jaroslav and Bojić-Trbojević, Žanka and Vacek, Jan and Llorente, Alicia and Antohe, Felicia and Suica, Viorel and Suarez, Guillaume and T’Kindt, Ruben and Martin, Petra and Penque, Deborah and Martins, Ines Lanca and Bodoki, Ede and Iacob, Bogdan-Cezar and Aydindogan, Eda and Timur, Suna and Allinson, John and Sutton, Christopher and Luider, Theo and Wittfooth, Saara and Sammar, Marei",
year = "2020",
abstract = "Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.",
journal = "Expert Review of Proteomics",
title = "Analytical techniques for multiplex analysis of protein biomarkers",
volume = "17",
number = "4",
pages = "257-273",
doi = "10.1080/14789450.2020.1763174"
}

Van Gool, A., Corrales, F., Čolović, M. B., Krstić, D. Z., Oliver-Martos, B., Martínez-Cáceres, E., Jakasa, I., Gajski, G., Brun, V., Kyriacou, K., Burzynska-Pedziwiatr, I., Wozniak, L. A., Nierkens, S., Pascual García, C., Katrlik, J., Bojić-Trbojević, Ž., Vacek, J., Llorente, A., Antohe, F., Suica, V., Suarez, G., T’Kindt, R., Martin, P., Penque, D., Martins, I. L., Bodoki, E., Iacob, B., Aydindogan, E., Timur, S., Allinson, J., Sutton, C., Luider, T., Wittfooth, S.,& Sammar, M.. (2020). Analytical techniques for multiplex analysis of protein biomarkers. in Expert Review of Proteomics, 17(4), 257-273.
https://doi.org/10.1080/14789450.2020.1763174

Van Gool A, Corrales F, Čolović MB, Krstić DZ, Oliver-Martos B, Martínez-Cáceres E, Jakasa I, Gajski G, Brun V, Kyriacou K, Burzynska-Pedziwiatr I, Wozniak LA, Nierkens S, Pascual García C, Katrlik J, Bojić-Trbojević Ž, Vacek J, Llorente A, Antohe F, Suica V, Suarez G, T’Kindt R, Martin P, Penque D, Martins IL, Bodoki E, Iacob B, Aydindogan E, Timur S, Allinson J, Sutton C, Luider T, Wittfooth S, Sammar M. Analytical techniques for multiplex analysis of protein biomarkers. in Expert Review of Proteomics. 2020;17(4):257-273.
doi:10.1080/14789450.2020.1763174 .

Van Gool, Alain, Corrales, Fernado, Čolović, Mirjana B., Krstić, Danijela Z., Oliver-Martos, Begona, Martínez-Cáceres, Eva, Jakasa, Ivone, Gajski, Goran, Brun, Virginie, Kyriacou, Kyriacos, Burzynska-Pedziwiatr, Izabela, Wozniak, Lucyna Alicja, Nierkens, Stephan, Pascual García, César, Katrlik, Jaroslav, Bojić-Trbojević, Žanka, Vacek, Jan, Llorente, Alicia, Antohe, Felicia, Suica, Viorel, Suarez, Guillaume, T’Kindt, Ruben, Martin, Petra, Penque, Deborah, Martins, Ines Lanca, Bodoki, Ede, Iacob, Bogdan-Cezar, Aydindogan, Eda, Timur, Suna, Allinson, John, Sutton, Christopher, Luider, Theo, Wittfooth, Saara, Sammar, Marei, "Analytical techniques for multiplex analysis of protein biomarkers" in Expert Review of Proteomics, 17, no. 4 (2020):257-273,
https://doi.org/10.1080/14789450.2020.1763174 . .