TY  - CONF
AU  - Matić, I.
AU  - Petrović, Nina
AU  - Tadić, V.
AU  - Maksimović, S.
AU  - Stanojković, T.
AU  - Zizovic, I.
PY  - 2023
UR  - https://web.archive.org/web/20231218093802/https://nwm.covr.be/EACR2023abstracts/data/HtmlApp/main.html
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12255
AB  - Introduction  The plant species belonging to the large genus Helichrysum are a valuable source of bioactive compounds. The aim of this study was to investigate the anticancer properties of the two Helichrysum italicum extracts obtained from plant material by supercritical CO2 extraction. Material and Methods  The cytotoxicity of the extracts was examined against six human cancer cell lines: cervical adenocarcinoma HeLa, lung carcinoma A549, prostate adenocarcinoma PC-3, breast adenocarcinoma MCF-7, melanoma A375, and chronic myelogenous leukemia K562, as well as against two human normal cell lines: lung fibroblasts MRC-5 and keratinocytes HaCaT. Cell cycle analysis was performed by flow cytometry. Gene and microRNA expression levels were measured by RT-qPCR. Results and Discussions  The H. italicum extracts exerted concentration-dependent and selective cytotoxic effects on cancer cells. HeLa, A375, and K562 cells were the most sensitive to the cytotoxic activity of both extracts. The extracts showed lower intensities of cytotoxic activity against normal HaCaT cells when compared with intensities of activity against K562, A375, and HeLa cells. An increases in the percentages of cells within subG1 and S phases of the cell cycle were observed in HeLa cells incubated for 24 h with IC50 and 2IC50 concentrations of the extracts in comparison with control cells. A similar effect on the cell cycle phase distribution was seen in A375 cells after 24 h incubation. The G2/M phase arrest was also detected in A375 cells exposed to IC50 concentrations of the extracts. Both extracts induced apoptosis in HeLa cells through the activation of effector caspase-3. The extracts triggered apoptosis through the intrinsic pathway mediated by caspase-9 and the extrinsic pathway mediated by caspase-8. Each of the extracts decreased expression levels of MMP2 in HeLa cells, slightly increased levels of MMP9, and increased levels of VEGFA. Up-regulations of genes involved in cancer invasion and progression, MMP2, MMP9, and VEGFA, were observed in A375 cells after 24 h treatment with extracts. The H. italicum extracts increased the expression levels of miR-16, miR-21, and miR-155 in HeLa cells, and decreased the expression levels of these miRNAs in A375 cells, which could be explained by their cancer-specific roles and signatures. Conclusion  The results of this study point out promising cancer-suppressive properties of Helichrysum italicum extracts rich in bioactive phytochemicals.
PB  - EACR - European Association for Cancer Research
C3  - EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts
T1  - Anticancer potential of two Helichrysum italicum extracts obtained by supercritical CO2 extraction
SP  - P-0155
EP  - P-0155
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12255
ER  - 

@conference{
author = "Matić, I. and Petrović, Nina and Tadić, V. and Maksimović, S. and Stanojković, T. and Zizovic, I.",
year = "2023",
abstract = "Introduction  The plant species belonging to the large genus Helichrysum are a valuable source of bioactive compounds. The aim of this study was to investigate the anticancer properties of the two Helichrysum italicum extracts obtained from plant material by supercritical CO2 extraction. Material and Methods  The cytotoxicity of the extracts was examined against six human cancer cell lines: cervical adenocarcinoma HeLa, lung carcinoma A549, prostate adenocarcinoma PC-3, breast adenocarcinoma MCF-7, melanoma A375, and chronic myelogenous leukemia K562, as well as against two human normal cell lines: lung fibroblasts MRC-5 and keratinocytes HaCaT. Cell cycle analysis was performed by flow cytometry. Gene and microRNA expression levels were measured by RT-qPCR. Results and Discussions  The H. italicum extracts exerted concentration-dependent and selective cytotoxic effects on cancer cells. HeLa, A375, and K562 cells were the most sensitive to the cytotoxic activity of both extracts. The extracts showed lower intensities of cytotoxic activity against normal HaCaT cells when compared with intensities of activity against K562, A375, and HeLa cells. An increases in the percentages of cells within subG1 and S phases of the cell cycle were observed in HeLa cells incubated for 24 h with IC50 and 2IC50 concentrations of the extracts in comparison with control cells. A similar effect on the cell cycle phase distribution was seen in A375 cells after 24 h incubation. The G2/M phase arrest was also detected in A375 cells exposed to IC50 concentrations of the extracts. Both extracts induced apoptosis in HeLa cells through the activation of effector caspase-3. The extracts triggered apoptosis through the intrinsic pathway mediated by caspase-9 and the extrinsic pathway mediated by caspase-8. Each of the extracts decreased expression levels of MMP2 in HeLa cells, slightly increased levels of MMP9, and increased levels of VEGFA. Up-regulations of genes involved in cancer invasion and progression, MMP2, MMP9, and VEGFA, were observed in A375 cells after 24 h treatment with extracts. The H. italicum extracts increased the expression levels of miR-16, miR-21, and miR-155 in HeLa cells, and decreased the expression levels of these miRNAs in A375 cells, which could be explained by their cancer-specific roles and signatures. Conclusion  The results of this study point out promising cancer-suppressive properties of Helichrysum italicum extracts rich in bioactive phytochemicals.",
publisher = "EACR - European Association for Cancer Research",
journal = "EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts",
title = "Anticancer potential of two Helichrysum italicum extracts obtained by supercritical CO2 extraction",
pages = "P-0155-P-0155",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12255"
}

Matić, I., Petrović, N., Tadić, V., Maksimović, S., Stanojković, T.,& Zizovic, I.. (2023). Anticancer potential of two Helichrysum italicum extracts obtained by supercritical CO2 extraction. in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts
EACR - European Association for Cancer Research., P-0155-P-0155.
https://hdl.handle.net/21.15107/rcub_vinar_12255

Matić I, Petrović N, Tadić V, Maksimović S, Stanojković T, Zizovic I. Anticancer potential of two Helichrysum italicum extracts obtained by supercritical CO2 extraction. in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts. 2023;:P-0155-P-0155.
https://hdl.handle.net/21.15107/rcub_vinar_12255 .

Matić, I., Petrović, Nina, Tadić, V., Maksimović, S., Stanojković, T., Zizovic, I., "Anticancer potential of two Helichrysum italicum extracts obtained by supercritical CO2 extraction" in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts (2023):P-0155-P-0155,
https://hdl.handle.net/21.15107/rcub_vinar_12255 .

TY  - CONF
AU  - Pašić, I.
AU  - Matić, I.
AU  - Petrović, Nina
AU  - Preljević, K.
AU  - Stanojković, T.
AU  - Perović, S.
PY  - 2023
UR  - https://web.archive.org/web/20231218093802/https://nwm.covr.be/EACR2023abstracts/data/HtmlApp/main.html
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12256
AB  - Introduction  The phytochemicals present in essential oils derived from aromatic plants of genus Thymus have been reported to exert antioxidant, anti-inflammatory, and anticancer effects. The aim of the research was to examine the cytotoxic activity and the mechanisms of anticancer action of the two essential oils obtained from Thymus vulgaris and Thymus serpyllum grown in Montenegro. Material and Methods  The cytotoxic activity was determined against four human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, and lung carcinoma A549, as well as against normal lung fibroblasts MRC-5 by MTT assay. The cell cycle phase distribution of HeLa cells and the potential activation of caspase-3, caspase-8, and caspase-9 were investigated by flow cytometry. Gene and microRNA expression levels in HeLa cells were measured using RT-qPCR. The intracellular levels of reactive oxygen species (ROS) in MRC-5 cells were measured by flow cytometry. Results and Discussions  Both essential oils exerted strong cytotoxicity against cancer cell lines with IC50 concentrations in the range from 0,20 to 0,24 µL/mL for T. vulgaris and from 0.32 to 0.49 µL/mL for T. serpyllum. Strong cytotoxicity was observed against lung fibroblasts MRC-5. The remarkable increases in the percentage of HeLa cells in the subG1 phase of the cell cycle after 24 h treatment with T. vulgaris and T. serpyllum essential oils were observed in comparison to the control cells. Pretreatment of HeLa cells with caspase inhibitors showed that T. vulgaris oil induced apoptotic cell death through caspase-3 and caspase-8, while T. serpyllum oil induced apoptosis through caspase-3 activation. Both essential oils decreased intracellular ROS levels in MRC-5 cells and reduced levels of oxidative stress induced by hydrogen peroxide. The treatment of HeLa cells with T. vulgaris oil lowered the MMP2 expression levels, increased MMP9 and VEGFA levels when compared with control cells, while T. serpyllum oil decreased the levels of MMP2 and MMP9, and increased VEGFA levels. HeLa cells treated with T. vulgaris oil had increased levels of miR-21, miR-16, and miR-34a, while cells treated with T. serpyllum oil had lower miR-16 and miR-34a levels, but higher miR-21 levels. Conclusion  These initial findings suggest that Thymus essential oils might have significant potential as cancer-chemopreventive and cancer-therapeutic agents, but further studies are necessary to evaluate their in vivo efficacy and safety.
PB  - EACR - European Association for Cancer Research
C3  - EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts
T1  - Anticancer effects of Thymus vulgaris and Thymus serpyllum essential oils from Montenegro
SP  - P-0111
EP  - P-0111
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12256
ER  - 

@conference{
author = "Pašić, I. and Matić, I. and Petrović, Nina and Preljević, K. and Stanojković, T. and Perović, S.",
year = "2023",
abstract = "Introduction  The phytochemicals present in essential oils derived from aromatic plants of genus Thymus have been reported to exert antioxidant, anti-inflammatory, and anticancer effects. The aim of the research was to examine the cytotoxic activity and the mechanisms of anticancer action of the two essential oils obtained from Thymus vulgaris and Thymus serpyllum grown in Montenegro. Material and Methods  The cytotoxic activity was determined against four human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, and lung carcinoma A549, as well as against normal lung fibroblasts MRC-5 by MTT assay. The cell cycle phase distribution of HeLa cells and the potential activation of caspase-3, caspase-8, and caspase-9 were investigated by flow cytometry. Gene and microRNA expression levels in HeLa cells were measured using RT-qPCR. The intracellular levels of reactive oxygen species (ROS) in MRC-5 cells were measured by flow cytometry. Results and Discussions  Both essential oils exerted strong cytotoxicity against cancer cell lines with IC50 concentrations in the range from 0,20 to 0,24 µL/mL for T. vulgaris and from 0.32 to 0.49 µL/mL for T. serpyllum. Strong cytotoxicity was observed against lung fibroblasts MRC-5. The remarkable increases in the percentage of HeLa cells in the subG1 phase of the cell cycle after 24 h treatment with T. vulgaris and T. serpyllum essential oils were observed in comparison to the control cells. Pretreatment of HeLa cells with caspase inhibitors showed that T. vulgaris oil induced apoptotic cell death through caspase-3 and caspase-8, while T. serpyllum oil induced apoptosis through caspase-3 activation. Both essential oils decreased intracellular ROS levels in MRC-5 cells and reduced levels of oxidative stress induced by hydrogen peroxide. The treatment of HeLa cells with T. vulgaris oil lowered the MMP2 expression levels, increased MMP9 and VEGFA levels when compared with control cells, while T. serpyllum oil decreased the levels of MMP2 and MMP9, and increased VEGFA levels. HeLa cells treated with T. vulgaris oil had increased levels of miR-21, miR-16, and miR-34a, while cells treated with T. serpyllum oil had lower miR-16 and miR-34a levels, but higher miR-21 levels. Conclusion  These initial findings suggest that Thymus essential oils might have significant potential as cancer-chemopreventive and cancer-therapeutic agents, but further studies are necessary to evaluate their in vivo efficacy and safety.",
publisher = "EACR - European Association for Cancer Research",
journal = "EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts",
title = "Anticancer effects of Thymus vulgaris and Thymus serpyllum essential oils from Montenegro",
pages = "P-0111-P-0111",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12256"
}

Pašić, I., Matić, I., Petrović, N., Preljević, K., Stanojković, T.,& Perović, S.. (2023). Anticancer effects of Thymus vulgaris and Thymus serpyllum essential oils from Montenegro. in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts
EACR - European Association for Cancer Research., P-0111-P-0111.
https://hdl.handle.net/21.15107/rcub_vinar_12256

Pašić I, Matić I, Petrović N, Preljević K, Stanojković T, Perović S. Anticancer effects of Thymus vulgaris and Thymus serpyllum essential oils from Montenegro. in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts. 2023;:P-0111-P-0111.
https://hdl.handle.net/21.15107/rcub_vinar_12256 .

Pašić, I., Matić, I., Petrović, Nina, Preljević, K., Stanojković, T., Perović, S., "Anticancer effects of Thymus vulgaris and Thymus serpyllum essential oils from Montenegro" in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts (2023):P-0111-P-0111,
https://hdl.handle.net/21.15107/rcub_vinar_12256 .