TY  - JOUR
AU  - Kamceva, Tina
AU  - Flemmig, Joerg
AU  - Damnjanović, Bojana
AU  - Arnhold, Juergen
AU  - Mijatovic, Aleksandar
AU  - Petković, Marijana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4514
AB  - Pancreatic phospholipase A(2) (PLA(2)) plays an important role in cellular homeostasis as well as in the process of carcinogenesis. Effects of metallo-drugs used as chemotherapeutics on the activity of this enzyme are unknown. In this work, the interaction between porcine pancreatic PLA(2) and two selected transition metal complexes-tetrachloro(bipyridine) platinum(IV) ([PtCl4(bipy)]) and dichloro (bipyridine) ruthenium(III)chloride ([RuCl2(bipy)(2)]Cl)-was studied. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and fluorescence spectroscopy have been used to analyse the enzyme activity in the absence and presence of metal complexes and to verify potential binding of these drugs to the enzyme. The tested metal complexes decreased the activity of phospholipase A(2) in an uncompetitive inhibition mode. A binding of the ruthenium complex near the active site of the enzyme could be evidenced and possible modes of interaction are discussed.
T2  - Metallomics
T1  - Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)
VL  - 3
IS  - 10
SP  - 1056
EP  - 1063
DO  - 10.1039/c1mt00088h
ER  - 

@article{
author = "Kamceva, Tina and Flemmig, Joerg and Damnjanović, Bojana and Arnhold, Juergen and Mijatovic, Aleksandar and Petković, Marijana",
year = "2011",
abstract = "Pancreatic phospholipase A(2) (PLA(2)) plays an important role in cellular homeostasis as well as in the process of carcinogenesis. Effects of metallo-drugs used as chemotherapeutics on the activity of this enzyme are unknown. In this work, the interaction between porcine pancreatic PLA(2) and two selected transition metal complexes-tetrachloro(bipyridine) platinum(IV) ([PtCl4(bipy)]) and dichloro (bipyridine) ruthenium(III)chloride ([RuCl2(bipy)(2)]Cl)-was studied. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and fluorescence spectroscopy have been used to analyse the enzyme activity in the absence and presence of metal complexes and to verify potential binding of these drugs to the enzyme. The tested metal complexes decreased the activity of phospholipase A(2) in an uncompetitive inhibition mode. A binding of the ruthenium complex near the active site of the enzyme could be evidenced and possible modes of interaction are discussed.",
journal = "Metallomics",
title = "Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)",
volume = "3",
number = "10",
pages = "1056-1063",
doi = "10.1039/c1mt00088h"
}

Kamceva, T., Flemmig, J., Damnjanović, B., Arnhold, J., Mijatovic, A.,& Petković, M.. (2011). Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2). in Metallomics, 3(10), 1056-1063.
https://doi.org/10.1039/c1mt00088h

Kamceva T, Flemmig J, Damnjanović B, Arnhold J, Mijatovic A, Petković M. Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2). in Metallomics. 2011;3(10):1056-1063.
doi:10.1039/c1mt00088h .

Kamceva, Tina, Flemmig, Joerg, Damnjanović, Bojana, Arnhold, Juergen, Mijatovic, Aleksandar, Petković, Marijana, "Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)" in Metallomics, 3, no. 10 (2011):1056-1063,
https://doi.org/10.1039/c1mt00088h . .

TY  - JOUR
AU  - Damnjanović, Bojana
AU  - Petrović, Biljana
AU  - Dimitrić-Marković, Jasmina
AU  - Petković, Marijana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4637
AB  - In this work, the matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectra of two cationic complexes, i.e., [PdCl(dien)]Cl and [Ru(en)(7)Cl-2]Cl, acquired under different conditions were analyzed. The spectra were recorded with three matrices with or without trifluoroacetic acid (TFA), i.e., two traditional matrices, i.e., 2,5-dihydroxybenzoic acid and alpha-cyano-hydroxycinnamic acid, and one flavonoid, quercetin. The spectra acquired with quercetin appeared to be the simplest, whereas in the spectra obtained with other matrices, peaks arising either from the addition of matrix molecules or from the fragmentation products were detectable. Addition of TFA did not complicate the spectra of the Pd(II) and Ru(III) complexes when the traditional matrices were used. On the other hand, the spectra of Pd complex were simpler, whereas the addition of TFA in the case of the Ru complex resulted in a higher number of peaks, some of which could not be identified. Taken together, the results of this study once more emphasize the differences arising in the MALDI-TOF mass spectra of transition metal complexes in dependence on the applied matrix.
T2  - Journal of the Serbian Chemical Society
T1  - Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices
VL  - 76
IS  - 12
SP  - 1687
EP  - 1701
DO  - 10.2298/JSC110201145D
ER  - 

@article{
author = "Damnjanović, Bojana and Petrović, Biljana and Dimitrić-Marković, Jasmina and Petković, Marijana",
year = "2011",
abstract = "In this work, the matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectra of two cationic complexes, i.e., [PdCl(dien)]Cl and [Ru(en)(7)Cl-2]Cl, acquired under different conditions were analyzed. The spectra were recorded with three matrices with or without trifluoroacetic acid (TFA), i.e., two traditional matrices, i.e., 2,5-dihydroxybenzoic acid and alpha-cyano-hydroxycinnamic acid, and one flavonoid, quercetin. The spectra acquired with quercetin appeared to be the simplest, whereas in the spectra obtained with other matrices, peaks arising either from the addition of matrix molecules or from the fragmentation products were detectable. Addition of TFA did not complicate the spectra of the Pd(II) and Ru(III) complexes when the traditional matrices were used. On the other hand, the spectra of Pd complex were simpler, whereas the addition of TFA in the case of the Ru complex resulted in a higher number of peaks, some of which could not be identified. Taken together, the results of this study once more emphasize the differences arising in the MALDI-TOF mass spectra of transition metal complexes in dependence on the applied matrix.",
journal = "Journal of the Serbian Chemical Society",
title = "Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices",
volume = "76",
number = "12",
pages = "1687-1701",
doi = "10.2298/JSC110201145D"
}

Damnjanović, B., Petrović, B., Dimitrić-Marković, J.,& Petković, M.. (2011). Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices. in Journal of the Serbian Chemical Society, 76(12), 1687-1701.
https://doi.org/10.2298/JSC110201145D

Damnjanović B, Petrović B, Dimitrić-Marković J, Petković M. Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices. in Journal of the Serbian Chemical Society. 2011;76(12):1687-1701.
doi:10.2298/JSC110201145D .

Damnjanović, Bojana, Petrović, Biljana, Dimitrić-Marković, Jasmina, Petković, Marijana, "Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices" in Journal of the Serbian Chemical Society, 76, no. 12 (2011):1687-1701,
https://doi.org/10.2298/JSC110201145D . .

TY  - JOUR
AU  - Damnjanović, Bojana
AU  - Kamceva, Tina
AU  - Petrović, Biljana
AU  - Bugarčić, Živadin D.
AU  - Petković, Marijana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4237
AB  - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently established as a powerful, soft ionization technique for the analysis of both transition metal complexes, which are used as metallo-drugs in the therapy of various types of tumors, and biomolecules. Since some metal complexes absorb light in the UV range, it should be possible to analyse them without additional matrices, i.e. using LDI-TOF MS. In this study, the matrix-free approach was tested for the analysis of [PtCl(2)(dach)] (dichloride(1,2-diamincyclohexane) platinum(II)), [RuCl(2)(en)(2)]Cl (dichloridobis(ethylenediamine) ruthenium(III) chloride) and [RuCl(2)(bipy)(2)]Cl (bis(bipyridine)dichloridoruthenium(III) chloride) and the detection limit for these compounds was determined. In summary, the LDI-TOF mass spectra of [PtCl(2)(dach)] and [RuCl(2)(en)(2)]Cl are rather simple, whereas in the presence of 2,5-DHB as a matrix, additional peaks are generated. On the other hand, the standard MALDI-TOF mass spectrum of [RuCl(2)(bipy)(2)]Cl exhibits only one peak arising from the complex, in contrast to six peaks detectable in the LDI-TOF mass spectrum. The detection limit in the MALDI-TOF MS analysis of [PtCl(2)(dach)] and [RuCl(2)(bipy)(2)]Cl complexes was lower than that determined in LDI-TOF MS. Taking all into account, in this paper, we have demonstrated some advantages and drawbacks of the matrix-free LDI-TOF mass spectrometric analysis of transition metal complexes.
T2  - Analytical Methods
T1  - Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes
VL  - 3
IS  - 2
SP  - 400
EP  - 407
DO  - 10.1039/c0ay00517g
ER  - 

@article{
author = "Damnjanović, Bojana and Kamceva, Tina and Petrović, Biljana and Bugarčić, Živadin D. and Petković, Marijana",
year = "2011",
abstract = "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently established as a powerful, soft ionization technique for the analysis of both transition metal complexes, which are used as metallo-drugs in the therapy of various types of tumors, and biomolecules. Since some metal complexes absorb light in the UV range, it should be possible to analyse them without additional matrices, i.e. using LDI-TOF MS. In this study, the matrix-free approach was tested for the analysis of [PtCl(2)(dach)] (dichloride(1,2-diamincyclohexane) platinum(II)), [RuCl(2)(en)(2)]Cl (dichloridobis(ethylenediamine) ruthenium(III) chloride) and [RuCl(2)(bipy)(2)]Cl (bis(bipyridine)dichloridoruthenium(III) chloride) and the detection limit for these compounds was determined. In summary, the LDI-TOF mass spectra of [PtCl(2)(dach)] and [RuCl(2)(en)(2)]Cl are rather simple, whereas in the presence of 2,5-DHB as a matrix, additional peaks are generated. On the other hand, the standard MALDI-TOF mass spectrum of [RuCl(2)(bipy)(2)]Cl exhibits only one peak arising from the complex, in contrast to six peaks detectable in the LDI-TOF mass spectrum. The detection limit in the MALDI-TOF MS analysis of [PtCl(2)(dach)] and [RuCl(2)(bipy)(2)]Cl complexes was lower than that determined in LDI-TOF MS. Taking all into account, in this paper, we have demonstrated some advantages and drawbacks of the matrix-free LDI-TOF mass spectrometric analysis of transition metal complexes.",
journal = "Analytical Methods",
title = "Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes",
volume = "3",
number = "2",
pages = "400-407",
doi = "10.1039/c0ay00517g"
}

Damnjanović, B., Kamceva, T., Petrović, B., Bugarčić, Ž. D.,& Petković, M.. (2011). Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes. in Analytical Methods, 3(2), 400-407.
https://doi.org/10.1039/c0ay00517g

Damnjanović B, Kamceva T, Petrović B, Bugarčić ŽD, Petković M. Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes. in Analytical Methods. 2011;3(2):400-407.
doi:10.1039/c0ay00517g .

Damnjanović, Bojana, Kamceva, Tina, Petrović, Biljana, Bugarčić, Živadin D., Petković, Marijana, "Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes" in Analytical Methods, 3, no. 2 (2011):400-407,
https://doi.org/10.1039/c0ay00517g . .