TY  - JOUR
AU  - Vujicic, Ana Djordjevic
AU  - Gemović, Branislava S.
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Veljković, Nevena V.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5960
AB  - Background/Aim. High sera reactivity with a peptide derived from human immunodeficiency virus HIV-I envelope protein gp120, NMI., correlate with non-progressive HIV-1 infection and also may have protective role in breast and prostate cancer. We also detected a low NTM1 reactive antibodies titer in healthy HIV negative sera and showed that antibody levels can be significantly increased with vigorous physical activity. However, the immune system seems to be unresponsive or tolerant to this peptide, implicating that the NTM1 sequence encompasses or overlaps a certain innate immune epitope. The aim of this study was to present evidences that NTM1 binding antibodies are components of innate immune humoral response, by confirming their presence in sera of newborn babies. For this purpose we collected a set of 225 innate antigen sequences reported in the literature and screened it for candidate antigens with the highest sequence and spectral similarity to NTM1 derived from HIV-1 gp120. Methods. Sera from 18 newborns were tested using ELISA, with peptide NTM1. Sequences from innate antigen database were aligned by an EMBOSS Water bioinformatics tool. Results. We identified NTM1 reactive antibodies in sera of HIV negative newborn babies. Further, in order to identify which of already known innate antigens are the most similar to NTM1 peptide we screened innate immune antigen sequence database collected from the literature. This screening revealed that the most similar sequence are ribonucleoproteins RO60, in addition to previously identified N-terminus of vasoactive intestinal peptide. Conclusion. The results of this study confirm the hypothesis that NTM1 recognizing antibodies are a part of humoral innate immune response. Further, computational similarity screening revealed a vasoactive intestinal peptide and RO60 as the most similar sequences and the strongest candidate antigens. In the light of the presented results, it is appealing that testing blood reactivity at birth, with specific innate antigens, particularly a vasoactive intestinal peptide, can reveal the potential to develop- or boost protective immune response in breast and prostate cancer and HIV infection later in life.
T2  - Vojnosanitetski pregled
T1  - Natural autoantibodies in healthy neonatals recognizing a peptide derived from the second conserved region of HIV-1 gp120
VL  - 71
IS  - 4
SP  - 352
EP  - 361
DO  - 10.2298/VSP1404352D
ER  - 

@article{
author = "Vujicic, Ana Djordjevic and Gemović, Branislava S. and Veljković, Veljko and Glišić, Sanja and Veljković, Nevena V.",
year = "2014",
abstract = "Background/Aim. High sera reactivity with a peptide derived from human immunodeficiency virus HIV-I envelope protein gp120, NMI., correlate with non-progressive HIV-1 infection and also may have protective role in breast and prostate cancer. We also detected a low NTM1 reactive antibodies titer in healthy HIV negative sera and showed that antibody levels can be significantly increased with vigorous physical activity. However, the immune system seems to be unresponsive or tolerant to this peptide, implicating that the NTM1 sequence encompasses or overlaps a certain innate immune epitope. The aim of this study was to present evidences that NTM1 binding antibodies are components of innate immune humoral response, by confirming their presence in sera of newborn babies. For this purpose we collected a set of 225 innate antigen sequences reported in the literature and screened it for candidate antigens with the highest sequence and spectral similarity to NTM1 derived from HIV-1 gp120. Methods. Sera from 18 newborns were tested using ELISA, with peptide NTM1. Sequences from innate antigen database were aligned by an EMBOSS Water bioinformatics tool. Results. We identified NTM1 reactive antibodies in sera of HIV negative newborn babies. Further, in order to identify which of already known innate antigens are the most similar to NTM1 peptide we screened innate immune antigen sequence database collected from the literature. This screening revealed that the most similar sequence are ribonucleoproteins RO60, in addition to previously identified N-terminus of vasoactive intestinal peptide. Conclusion. The results of this study confirm the hypothesis that NTM1 recognizing antibodies are a part of humoral innate immune response. Further, computational similarity screening revealed a vasoactive intestinal peptide and RO60 as the most similar sequences and the strongest candidate antigens. In the light of the presented results, it is appealing that testing blood reactivity at birth, with specific innate antigens, particularly a vasoactive intestinal peptide, can reveal the potential to develop- or boost protective immune response in breast and prostate cancer and HIV infection later in life.",
journal = "Vojnosanitetski pregled",
title = "Natural autoantibodies in healthy neonatals recognizing a peptide derived from the second conserved region of HIV-1 gp120",
volume = "71",
number = "4",
pages = "352-361",
doi = "10.2298/VSP1404352D"
}

Vujicic, A. D., Gemović, B. S., Veljković, V., Glišić, S.,& Veljković, N. V.. (2014). Natural autoantibodies in healthy neonatals recognizing a peptide derived from the second conserved region of HIV-1 gp120. in Vojnosanitetski pregled, 71(4), 352-361.
https://doi.org/10.2298/VSP1404352D

Vujicic AD, Gemović BS, Veljković V, Glišić S, Veljković NV. Natural autoantibodies in healthy neonatals recognizing a peptide derived from the second conserved region of HIV-1 gp120. in Vojnosanitetski pregled. 2014;71(4):352-361.
doi:10.2298/VSP1404352D .

Vujicic, Ana Djordjevic, Gemović, Branislava S., Veljković, Veljko, Glišić, Sanja, Veljković, Nevena V., "Natural autoantibodies in healthy neonatals recognizing a peptide derived from the second conserved region of HIV-1 gp120" in Vojnosanitetski pregled, 71, no. 4 (2014):352-361,
https://doi.org/10.2298/VSP1404352D . .

TY  - JOUR
AU  - Vasiljevic, Nada
AU  - Veljković, Nevena V.
AU  - Kosec, Tatjana
AU  - Ma, Xue-Zhong
AU  - Glišić, Sanja
AU  - Prljić, Jelena
AU  - Vujicic, Ana Djordjevic
AU  - Markovic, Ljiljana
AU  - Branch, Donald R.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4259
AB  - Natural autoantibodies (NAbs) are continually produced throughout life and have an ability to recognize self and altered self, as well as foreign antigens, by recognizing cellular pattern recognition receptors. Sometimes NAb specificity demonstrates overlap between human and pathologic proteomes. This information can be useful in selecting target sequences for screening purposes. In this study we undertook a multi-step bioinformatics search to predict a virus-derived peptide that can be recognized by NAbs in sera of uninfected individuals. We selected protein hepatitis C virus (HCV) NS5A as a target sequence, motivated by the fact that the HCV proteome is characterized by extensive sequence similarities to the human proteome, and because screening for anti-HCV antibodies, including anti-NS5A, is important clinically, particularly in screening of potential blood donors. The virus-specific peptide P1, and the homologous human peptide derived from enzyme-inducible nitric oxide synthase (iNOS), P2, exhibiting not only simple homology, but also complementarities of physicochemical patterns, were synthesized and 80 HCV-negative and 50 HCV-positive blood donor sera were tested by ELISA. These peptides reacted similarly (p LT 0.001) with HCV-negative sera, and in several cases the measured reactivity was significantly above the cut-off value of commercial anti-HCV screening assays. In HCV-positive sera, the titers of antibodies reactive with analyzed HCV NS5A peptide were not significantly increased (p LT 0.001) compared to host peptide, the implications of which are unclear, but may be consistent with these antibodies being naturally produced. Finally, we extended our bioinformatics analyses to the dataset of human self-binding sequences, and propose a general approach for the selection of specific diagnostic and screening antigens for use in immunoassays.
T2  - Viral Immunology
T1  - A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay
VL  - 24
IS  - 2
SP  - 69
EP  - 76
DO  - 10.1089/vim.2010.0107
ER  - 

@article{
author = "Vasiljevic, Nada and Veljković, Nevena V. and Kosec, Tatjana and Ma, Xue-Zhong and Glišić, Sanja and Prljić, Jelena and Vujicic, Ana Djordjevic and Markovic, Ljiljana and Branch, Donald R.",
year = "2011",
abstract = "Natural autoantibodies (NAbs) are continually produced throughout life and have an ability to recognize self and altered self, as well as foreign antigens, by recognizing cellular pattern recognition receptors. Sometimes NAb specificity demonstrates overlap between human and pathologic proteomes. This information can be useful in selecting target sequences for screening purposes. In this study we undertook a multi-step bioinformatics search to predict a virus-derived peptide that can be recognized by NAbs in sera of uninfected individuals. We selected protein hepatitis C virus (HCV) NS5A as a target sequence, motivated by the fact that the HCV proteome is characterized by extensive sequence similarities to the human proteome, and because screening for anti-HCV antibodies, including anti-NS5A, is important clinically, particularly in screening of potential blood donors. The virus-specific peptide P1, and the homologous human peptide derived from enzyme-inducible nitric oxide synthase (iNOS), P2, exhibiting not only simple homology, but also complementarities of physicochemical patterns, were synthesized and 80 HCV-negative and 50 HCV-positive blood donor sera were tested by ELISA. These peptides reacted similarly (p LT 0.001) with HCV-negative sera, and in several cases the measured reactivity was significantly above the cut-off value of commercial anti-HCV screening assays. In HCV-positive sera, the titers of antibodies reactive with analyzed HCV NS5A peptide were not significantly increased (p LT 0.001) compared to host peptide, the implications of which are unclear, but may be consistent with these antibodies being naturally produced. Finally, we extended our bioinformatics analyses to the dataset of human self-binding sequences, and propose a general approach for the selection of specific diagnostic and screening antigens for use in immunoassays.",
journal = "Viral Immunology",
title = "A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay",
volume = "24",
number = "2",
pages = "69-76",
doi = "10.1089/vim.2010.0107"
}

Vasiljevic, N., Veljković, N. V., Kosec, T., Ma, X., Glišić, S., Prljić, J., Vujicic, A. D., Markovic, L.,& Branch, D. R.. (2011). A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay. in Viral Immunology, 24(2), 69-76.
https://doi.org/10.1089/vim.2010.0107

Vasiljevic N, Veljković NV, Kosec T, Ma X, Glišić S, Prljić J, Vujicic AD, Markovic L, Branch DR. A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay. in Viral Immunology. 2011;24(2):69-76.
doi:10.1089/vim.2010.0107 .

Vasiljevic, Nada, Veljković, Nevena V., Kosec, Tatjana, Ma, Xue-Zhong, Glišić, Sanja, Prljić, Jelena, Vujicic, Ana Djordjevic, Markovic, Ljiljana, Branch, Donald R., "A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay" in Viral Immunology, 24, no. 2 (2011):69-76,
https://doi.org/10.1089/vim.2010.0107 . .