TY  - JOUR
AU  - Terzić, N
AU  - Vujčić, Miroslava T.
AU  - Ristić-Fira, Aleksandra
AU  - Krstić-Demonacos, Marija
AU  - Milanovic, D
AU  - Kanazir, Dušan T.
AU  - Ruždijić, Sabera
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2623
AB  - The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX treatment in an age-specific manner.
T2  - Journals of Gerontology. Series A: Biological Sciences and Medical Sciences
T1  - Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain
VL  - 58
IS  - 4
SP  - 297
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2623
ER  - 

@article{
author = "Terzić, N and Vujčić, Miroslava T. and Ristić-Fira, Aleksandra and Krstić-Demonacos, Marija and Milanovic, D and Kanazir, Dušan T. and Ruždijić, Sabera",
year = "2003",
abstract = "The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX treatment in an age-specific manner.",
journal = "Journals of Gerontology. Series A: Biological Sciences and Medical Sciences",
title = "Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain",
volume = "58",
number = "4",
pages = "297-303",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2623"
}

Terzić, N., Vujčić, M. T., Ristić-Fira, A., Krstić-Demonacos, M., Milanovic, D., Kanazir, D. T.,& Ruždijić, S.. (2003). Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain. in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 58(4), 297-303.
https://hdl.handle.net/21.15107/rcub_vinar_2623

Terzić N, Vujčić MT, Ristić-Fira A, Krstić-Demonacos M, Milanovic D, Kanazir DT, Ruždijić S. Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain. in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences. 2003;58(4):297-303.
https://hdl.handle.net/21.15107/rcub_vinar_2623 .

Terzić, N, Vujčić, Miroslava T., Ristić-Fira, Aleksandra, Krstić-Demonacos, Marija, Milanovic, D, Kanazir, Dušan T., Ruždijić, Sabera, "Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain" in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 58, no. 4 (2003):297-303,
https://hdl.handle.net/21.15107/rcub_vinar_2623 .