@conference{
author = "Nikšić, Valentina and Dukić, Miljana and Pirković, Andrea and Lazić, Vesna",
year = "2023",
abstract = "Cilj ovog rada je modifikacija površine nanočestica TiO2 bioaktivnim ligandima, kao što je dihidrokvercetin (DHQ), formiranjem interfacijalnog kompleksa sa prenosom naelektrisanja, kako bi se postigla njegova aktivacija pod vidljivom svetlošću. Neorganskoorganski hibridni nanokompozit TiO2 /DHQ okarakterisan je infracrvenom (FTIR) i refleksionom spektroskopijom. FTIR spektri su identifikovali C=O grupe flavonoida, O-H, C-O i C-O-C grupe fenola, potvrđujući prisustvo liganada na površini nanočestica TiO2 . Kubelka-Munk transformacijom spektara difuzione refleksije vidi se pomeraj ekscitacije /DHQ prema vidljivom delu spektra. Ispitivanje citotoksičnosti MTT testom urađeno je na zdravim ljudskim MRC-5 ćelijama, kao i na ljudskim HeLa ćelijama raka grlića materice. Takođe, H2DCFDA testom ispitan je efekat TiO2 /DHQ na proizvodnju reaktivnih vrsta kiseonika u MRC-5 ćelijama., This study aims to modify the surface of TiO2 nanoparticles with bioactive ligands, forming an interfacial charge transfer complex to achieve its activation under visible light. Dihydroquercetin (DHQ) is a catechol-type ligand with pronounced antioxidant and valuable biological performance. The inorganic-organic hybrid nanocomposite TiO2 /DHQ was characterized by Fourier transform infrared (FTIR) and Reflectance spectroscopy. FTIR spectra identified C=O stretching in flavones, O-H, C-O and C-O-C starching in phenolic compounds, demonstrating the presence of ligands on the surface of TiO2 nanoparticles. The Kubelka-Munk transformation of the diffuse reflection spectra shows a shift in the excitation of the TiO2 /DHQ towards the visible part of the spectrum. Cytotoxicity testing was performed on healthy human MRC-5 cells and human cervical cancer HeLa cells determined by MTT assay. Also, the effect of TiO2 /DHQ on the production of reactive oxygen species in MRC-5 cells was determined by the H2DCFDA assay.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings",
title = "Toksičnost nanočestica TiO2 modifikovanih dihidrokvarcetinom, Toxicity of TiO2 nanoparticles modified with dihydroquercetin",
pages = "146-146",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13039"
}