TY  - JOUR
AU  - Đokić, Vladimir
AU  - Janković, Svetlana
AU  - Labudović-Borović, Milica
AU  - Rakočević, Jelena T.
AU  - Stanišić, Jelena
AU  - Rajković, Jovana
AU  - Novaković, Radmila
AU  - Kostić, Milan
AU  - Đurić, Miloš
AU  - Gostimirović, Miloš
AU  - Gojković-Bukarica, Ljiljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9048
AB  - Voltage-gated potassium (K ) channels are the largest superfamily of potassium (K) channels. A variety of K channels are expressed in the vascular smooth muscle cells (SMC). Studies have shown that gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) cause various changes in the human umbilical vein (HUV). Recently, we have shown that 4-AP, a nonspecific K 1-4 channel inhibitor, significantly decreases vasorelaxation induced by K channel opener pinacidil in vascular SMCs of the HUVs from normal pregnancies, but not in GDM and PIH. The goal of this study was to provide more detailed insight in the K channel subtypes involved in pinacidil-induced vasodilation of HUVs, as well as to investigate potential alterations of their function and expression during GDM and PIH. Margatoxin, a specific blocker of K 1.2 and K 1.3 channels, significantly antagonized pinacidil-induced vasorelaxation in normal pregnancy, while in HUVs from GDM and PIH that was not the case, indicating damage of K 1.2 and K 1.3 channel function. Immunohistochemistry and Western blot revealed similar expression of K 1.2 channels in all groups. The expression of K 1.3 subunit was significantly decreased in PIH, while it remained unchanged in GDM compared to normal pregnancy. Phrixotoxin, specific blocker of K 4.2 and K 4.3 channels, did not antagonize response to pinacidil in any of the groups. The major novel findings show that margatoxin antagonized pinacidil-induced relaxation in normal pregnancy, but not in GDM and PIH. Decreased expression of K 1.3 channels in HUV during PIH may be important pathophysiological mechanism contributing to an increased risk of adverse pregnancy outcomes.
T2  - European Journal of Pharmacology
T1  - Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle
VL  - 882
SP  - 173281
DO  - 10.1016/j.ejphar.2020.173281
ER  - 

@article{
author = "Đokić, Vladimir and Janković, Svetlana and Labudović-Borović, Milica and Rakočević, Jelena T. and Stanišić, Jelena and Rajković, Jovana and Novaković, Radmila and Kostić, Milan and Đurić, Miloš and Gostimirović, Miloš and Gojković-Bukarica, Ljiljana",
year = "2020",
abstract = "Voltage-gated potassium (K ) channels are the largest superfamily of potassium (K) channels. A variety of K channels are expressed in the vascular smooth muscle cells (SMC). Studies have shown that gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) cause various changes in the human umbilical vein (HUV). Recently, we have shown that 4-AP, a nonspecific K 1-4 channel inhibitor, significantly decreases vasorelaxation induced by K channel opener pinacidil in vascular SMCs of the HUVs from normal pregnancies, but not in GDM and PIH. The goal of this study was to provide more detailed insight in the K channel subtypes involved in pinacidil-induced vasodilation of HUVs, as well as to investigate potential alterations of their function and expression during GDM and PIH. Margatoxin, a specific blocker of K 1.2 and K 1.3 channels, significantly antagonized pinacidil-induced vasorelaxation in normal pregnancy, while in HUVs from GDM and PIH that was not the case, indicating damage of K 1.2 and K 1.3 channel function. Immunohistochemistry and Western blot revealed similar expression of K 1.2 channels in all groups. The expression of K 1.3 subunit was significantly decreased in PIH, while it remained unchanged in GDM compared to normal pregnancy. Phrixotoxin, specific blocker of K 4.2 and K 4.3 channels, did not antagonize response to pinacidil in any of the groups. The major novel findings show that margatoxin antagonized pinacidil-induced relaxation in normal pregnancy, but not in GDM and PIH. Decreased expression of K 1.3 channels in HUV during PIH may be important pathophysiological mechanism contributing to an increased risk of adverse pregnancy outcomes.",
journal = "European Journal of Pharmacology",
title = "Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle",
volume = "882",
pages = "173281",
doi = "10.1016/j.ejphar.2020.173281"
}

Đokić, V., Janković, S., Labudović-Borović, M., Rakočević, J. T., Stanišić, J., Rajković, J., Novaković, R., Kostić, M., Đurić, M., Gostimirović, M.,& Gojković-Bukarica, L.. (2020). Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle. in European Journal of Pharmacology, 882, 173281.
https://doi.org/10.1016/j.ejphar.2020.173281

Đokić V, Janković S, Labudović-Borović M, Rakočević JT, Stanišić J, Rajković J, Novaković R, Kostić M, Đurić M, Gostimirović M, Gojković-Bukarica L. Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle. in European Journal of Pharmacology. 2020;882:173281.
doi:10.1016/j.ejphar.2020.173281 .

Đokić, Vladimir, Janković, Svetlana, Labudović-Borović, Milica, Rakočević, Jelena T., Stanišić, Jelena, Rajković, Jovana, Novaković, Radmila, Kostić, Milan, Đurić, Miloš, Gostimirović, Miloš, Gojković-Bukarica, Ljiljana, "Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle" in European Journal of Pharmacology, 882 (2020):173281,
https://doi.org/10.1016/j.ejphar.2020.173281 . .

TY  - JOUR
AU  - Rajković, Jovana
AU  - Perić, M.
AU  - Stanišić, Jelena
AU  - Novaković, Radmila
AU  - Đokić, Vladimir
AU  - Rakočević, Jelena T.
AU  - Tepavčević, Snežana
AU  - Labudović-Borović, Milica
AU  - Gostimirović, Miloš
AU  - Heinle, Helmut
AU  - Gojković-Bukarica, Ljiljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9054
AB  - Type 2 diabetes mellitus (T2DM) increases cardiovascular complications. Diabetic vascular dysfunction is associated with the reduced activity of the different smooth muscle potassium (K+) channels. Thus, the objective of our study was to investigate the role of the adenosine triphosphate (ATP)-sensitive K+ (KATP) channels in the relaxant effect of potassium channel opener, pinacidil on the human saphenous vein (HSV) obtained from the patients with and without T2DM. The rings of HSV without the endothelium, obtained from the patients who had undergone coronary bypass surgery, were mounted in an organ bath system and isometric tension was recorded. The relaxation of HSV, precontracted with phenylephrine, was produced by pinacidil. The expression of KATP subunits (Kir6.1, Kir6.2 and SUR2B) was detected by immunohistochemistry and Western blot. Pinacidil produces comparable effects on HSV in patients with and without T2DM. The suppression of pinacidil effect and its maximal relaxation by glibenclamide, selective blocker of KATP channels, was more pronounced on HSV in patients without T2DM. All three types of KATP subunits are expressed on the smooth muscle cells of HSV. While there are no differences in the expression of Kir6.1 and Kir6.2, the expression of SUR2B is lower in HSV in patients with T2DM. Pinacidil produced comparable KATP-dependent and -independent relaxation of the HSV in patients with/without T2DM. According to the effect of glibenclamide and the applied molecular analysis, presented findings demonstrated that diabetes mellitus was associated with the reduced expression of SUR2B subunit in the vascular smooth muscle of HSV.
T2  - Journal of physiology and pharmacology : an official journal of the Polish Physiological Society
T1  - The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus
VL  - 71
IS  - 1
DO  - 10.26402/jpp.2020.1.12
ER  - 

@article{
author = "Rajković, Jovana and Perić, M. and Stanišić, Jelena and Novaković, Radmila and Đokić, Vladimir and Rakočević, Jelena T. and Tepavčević, Snežana and Labudović-Borović, Milica and Gostimirović, Miloš and Heinle, Helmut and Gojković-Bukarica, Ljiljana",
year = "2020",
abstract = "Type 2 diabetes mellitus (T2DM) increases cardiovascular complications. Diabetic vascular dysfunction is associated with the reduced activity of the different smooth muscle potassium (K+) channels. Thus, the objective of our study was to investigate the role of the adenosine triphosphate (ATP)-sensitive K+ (KATP) channels in the relaxant effect of potassium channel opener, pinacidil on the human saphenous vein (HSV) obtained from the patients with and without T2DM. The rings of HSV without the endothelium, obtained from the patients who had undergone coronary bypass surgery, were mounted in an organ bath system and isometric tension was recorded. The relaxation of HSV, precontracted with phenylephrine, was produced by pinacidil. The expression of KATP subunits (Kir6.1, Kir6.2 and SUR2B) was detected by immunohistochemistry and Western blot. Pinacidil produces comparable effects on HSV in patients with and without T2DM. The suppression of pinacidil effect and its maximal relaxation by glibenclamide, selective blocker of KATP channels, was more pronounced on HSV in patients without T2DM. All three types of KATP subunits are expressed on the smooth muscle cells of HSV. While there are no differences in the expression of Kir6.1 and Kir6.2, the expression of SUR2B is lower in HSV in patients with T2DM. Pinacidil produced comparable KATP-dependent and -independent relaxation of the HSV in patients with/without T2DM. According to the effect of glibenclamide and the applied molecular analysis, presented findings demonstrated that diabetes mellitus was associated with the reduced expression of SUR2B subunit in the vascular smooth muscle of HSV.",
journal = "Journal of physiology and pharmacology : an official journal of the Polish Physiological Society",
title = "The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus",
volume = "71",
number = "1",
doi = "10.26402/jpp.2020.1.12"
}

Rajković, J., Perić, M., Stanišić, J., Novaković, R., Đokić, V., Rakočević, J. T., Tepavčević, S., Labudović-Borović, M., Gostimirović, M., Heinle, H.,& Gojković-Bukarica, L.. (2020). The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus. in Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 71(1).
https://doi.org/10.26402/jpp.2020.1.12

Rajković J, Perić M, Stanišić J, Novaković R, Đokić V, Rakočević JT, Tepavčević S, Labudović-Borović M, Gostimirović M, Heinle H, Gojković-Bukarica L. The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus. in Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. 2020;71(1).
doi:10.26402/jpp.2020.1.12 .

Rajković, Jovana, Perić, M., Stanišić, Jelena, Novaković, Radmila, Đokić, Vladimir, Rakočević, Jelena T., Tepavčević, Snežana, Labudović-Borović, Milica, Gostimirović, Miloš, Heinle, Helmut, Gojković-Bukarica, Ljiljana, "The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus" in Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 71, no. 1 (2020),
https://doi.org/10.26402/jpp.2020.1.12 . .

TY  - JOUR
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović-Borović, Milica
AU  - Borisev, Ivana
AU  - Novaković, Mirjana M.
AU  - Rakočević, Zlatko Lj.
AU  - Đorđević, Aleksandar N.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8649
AB  - Fullerenol C60(OH)24 with its spherical shape, symmetrical structure, 1 nm size and the ability to form polyionic nanoparticles in water solution, was used to synthesise a novel nanocomposite made of fullerenol nanoparticles (FNP) and iron ions (Fe2+). The FNP/Fe2+ nanocomposite was characterised by DLS and TEM-EDS analyses which have shown that the size distribution of FNP/Fe2+ stayed in the same scope as the size distribution of FNP, ranging from 11 to 60 nm. However, Fe2+ did affect the change of FNP’s zeta potential (− 49.2 mV), shifting it to more positive values (− 30.8 mV). In this study, it was assumed that FNP/Fe2+ could reduce the toxic effects of doxorubicin (Dox). Male Wistar rats were treated i.p. with FNP/Fe2+ nanocomposite 1 h prior to Dox treatment. At the subcellular level, the ultrastructural analysis revealed minor alterations sporadically displayed within the heart and liver tissues. Moreover, at the molecular level, the gene expressions analysis of mRNAs for catalase (heart and liver) and MnSOD (only liver) were significantly downregulated, indicating reduction in oxidative stress. Overall, the pretreatment with FNP/Fe2+ nanocomposite, followed by Dox application, significantly diminished harmful effects of the applied drug on the heart and liver, suggesting the potential protective effect of the nanocomposite on the healthy tissues. © 2019, Springer Nature B.V.
T2  - Journal of Nanoparticle Research
T1  - Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity
VL  - 21
IS  - 11
SP  - 239
DO  - 10.1007/s11051-019-4681-4
ER  - 

@article{
author = "Seke, Mariana and Petrović, Danijela and Labudović-Borović, Milica and Borisev, Ivana and Novaković, Mirjana M. and Rakočević, Zlatko Lj. and Đorđević, Aleksandar N.",
year = "2019",
abstract = "Fullerenol C60(OH)24 with its spherical shape, symmetrical structure, 1 nm size and the ability to form polyionic nanoparticles in water solution, was used to synthesise a novel nanocomposite made of fullerenol nanoparticles (FNP) and iron ions (Fe2+). The FNP/Fe2+ nanocomposite was characterised by DLS and TEM-EDS analyses which have shown that the size distribution of FNP/Fe2+ stayed in the same scope as the size distribution of FNP, ranging from 11 to 60 nm. However, Fe2+ did affect the change of FNP’s zeta potential (− 49.2 mV), shifting it to more positive values (− 30.8 mV). In this study, it was assumed that FNP/Fe2+ could reduce the toxic effects of doxorubicin (Dox). Male Wistar rats were treated i.p. with FNP/Fe2+ nanocomposite 1 h prior to Dox treatment. At the subcellular level, the ultrastructural analysis revealed minor alterations sporadically displayed within the heart and liver tissues. Moreover, at the molecular level, the gene expressions analysis of mRNAs for catalase (heart and liver) and MnSOD (only liver) were significantly downregulated, indicating reduction in oxidative stress. Overall, the pretreatment with FNP/Fe2+ nanocomposite, followed by Dox application, significantly diminished harmful effects of the applied drug on the heart and liver, suggesting the potential protective effect of the nanocomposite on the healthy tissues. © 2019, Springer Nature B.V.",
journal = "Journal of Nanoparticle Research",
title = "Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity",
volume = "21",
number = "11",
pages = "239",
doi = "10.1007/s11051-019-4681-4"
}

Seke, M., Petrović, D., Labudović-Borović, M., Borisev, I., Novaković, M. M., Rakočević, Z. Lj.,& Đorđević, A. N.. (2019). Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity. in Journal of Nanoparticle Research, 21(11), 239.
https://doi.org/10.1007/s11051-019-4681-4

Seke M, Petrović D, Labudović-Borović M, Borisev I, Novaković MM, Rakočević ZL, Đorđević AN. Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity. in Journal of Nanoparticle Research. 2019;21(11):239.
doi:10.1007/s11051-019-4681-4 .

Seke, Mariana, Petrović, Danijela, Labudović-Borović, Milica, Borisev, Ivana, Novaković, Mirjana M., Rakočević, Zlatko Lj., Đorđević, Aleksandar N., "Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity" in Journal of Nanoparticle Research, 21, no. 11 (2019):239,
https://doi.org/10.1007/s11051-019-4681-4 . .

TY  - JOUR
AU  - Zafirović, Sonja
AU  - Sudar-Milovanović, Emina
AU  - Obradović, Milan M.
AU  - Đorđević, Jelena D.
AU  - Jasnić, Nebojša
AU  - Labudović-Borović, Milica
AU  - Isenović, Esma R.
PY  - 2019
UR  - http://www.eurekaselect.com/159734/article
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8097
AB  - BACKGROUND: Oestradiol is an important regulatory factor with several positive effects on the cardiovascular (CV) system. We evaluated the molecular mechanism of the in vivo effects of oestradiol on the regulation of cardiac inducible nitric oxide (NO) synthase (iNOS) expression and activity. METHODS: Male Wistar rats were treated with oestradiol (40 mg/kg, intraperitoneally) and after 24 h the animals were sacrificed. The concentrations of NO and L-Arginine (L-Arg) were determined spectrophotometrically. For protein expressions of iNOS, p65 subunit of nuclear factor-κB (NFκB-p65), Ras homolog gene family-member A (RhoA), angiotensin II receptor type 1 (AT1R), insulin receptor substrate 1 (IRS-1), p85, p110 and protein kinase B (Akt), Western blot method was used. Coimmunoprecipitation was used for measuring the association of IRS-1 with the p85 subunit of phosphatidylinositol- 3-kinase (PI3K). The expression of iNOS messenger ribonucleic acid (mRNA) was measured with the quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemical analysis of the tissue was used to detect localization and expression of iNOS in heart tissue. RESULTS: Oestradiol treatment reduced L-Arg concentration (p<0.01), iNOS mRNA (p<0.01) and protein (p<0.001) expression, level of RhoA (p<0.05) and AT1R (p<0.001) protein. In contrast, plasma NO (p<0.05), Akt phosphorylation at Thr308 (p<0.05) and protein level of p85 (p<0.001) increased after oestradiol treatment. CONCLUSION: Our results suggest that oestradiol in vivo regulates cardiac iNOS expression via the PI3K/Akt signaling pathway, through attenuation of RhoA and AT1R.
T2  - Current Vascular Pharmacology
T1  - Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression
VL  - 17
IS  - 3
SP  - 307
EP  - 318
DO  - 10.2174/1570161116666180212142414
ER  - 

@article{
author = "Zafirović, Sonja and Sudar-Milovanović, Emina and Obradović, Milan M. and Đorđević, Jelena D. and Jasnić, Nebojša and Labudović-Borović, Milica and Isenović, Esma R.",
year = "2019",
abstract = "BACKGROUND: Oestradiol is an important regulatory factor with several positive effects on the cardiovascular (CV) system. We evaluated the molecular mechanism of the in vivo effects of oestradiol on the regulation of cardiac inducible nitric oxide (NO) synthase (iNOS) expression and activity. METHODS: Male Wistar rats were treated with oestradiol (40 mg/kg, intraperitoneally) and after 24 h the animals were sacrificed. The concentrations of NO and L-Arginine (L-Arg) were determined spectrophotometrically. For protein expressions of iNOS, p65 subunit of nuclear factor-κB (NFκB-p65), Ras homolog gene family-member A (RhoA), angiotensin II receptor type 1 (AT1R), insulin receptor substrate 1 (IRS-1), p85, p110 and protein kinase B (Akt), Western blot method was used. Coimmunoprecipitation was used for measuring the association of IRS-1 with the p85 subunit of phosphatidylinositol- 3-kinase (PI3K). The expression of iNOS messenger ribonucleic acid (mRNA) was measured with the quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemical analysis of the tissue was used to detect localization and expression of iNOS in heart tissue. RESULTS: Oestradiol treatment reduced L-Arg concentration (p<0.01), iNOS mRNA (p<0.01) and protein (p<0.001) expression, level of RhoA (p<0.05) and AT1R (p<0.001) protein. In contrast, plasma NO (p<0.05), Akt phosphorylation at Thr308 (p<0.05) and protein level of p85 (p<0.001) increased after oestradiol treatment. CONCLUSION: Our results suggest that oestradiol in vivo regulates cardiac iNOS expression via the PI3K/Akt signaling pathway, through attenuation of RhoA and AT1R.",
journal = "Current Vascular Pharmacology",
title = "Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression",
volume = "17",
number = "3",
pages = "307-318",
doi = "10.2174/1570161116666180212142414"
}

Zafirović, S., Sudar-Milovanović, E., Obradović, M. M., Đorđević, J. D., Jasnić, N., Labudović-Borović, M.,& Isenović, E. R.. (2019). Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression. in Current Vascular Pharmacology, 17(3), 307-318.
https://doi.org/10.2174/1570161116666180212142414

Zafirović S, Sudar-Milovanović E, Obradović MM, Đorđević JD, Jasnić N, Labudović-Borović M, Isenović ER. Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression. in Current Vascular Pharmacology. 2019;17(3):307-318.
doi:10.2174/1570161116666180212142414 .

Zafirović, Sonja, Sudar-Milovanović, Emina, Obradović, Milan M., Đorđević, Jelena D., Jasnić, Nebojša, Labudović-Borović, Milica, Isenović, Esma R., "Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression" in Current Vascular Pharmacology, 17, no. 3 (2019):307-318,
https://doi.org/10.2174/1570161116666180212142414 . .

TY  - JOUR
AU  - Đokić, Vladimir
AU  - Janković-Ražnatović, Svetlana
AU  - Novaković, Radmila
AU  - Kostić, Milan
AU  - Rajković, Jovana
AU  - Labudović-Borović, Milica
AU  - Rakočević, Jelena T.
AU  - Stanišić, Jelena
AU  - Đurić, Miloš
AU  - Gojković-Bukarica, Ljiljana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8626
AB  - Gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) can jeopardize mother and/or fetus. Vascular ATP-sensitive potassium (KATP) channels most likely participate in the processes of diabetes and hypertension. The aim of this research was to examine whether GDM and PIH cause changes in the expression and function of KATP channels in vascular smooth muscle of human umbilical vein (HUV). Western blot and immunohistochemistry detected significantly decreased expression of Kir6.1 subunit of KATP channels in GDM and PIH, while the expression of SUR2B was unchanged. In GDM, a K+ channel opener, pinacidil caused reduced relaxation of the endothelium-denuded HUVs compared to normal pregnancy. However, its effects in HUVs from PIH subjects were similar to normal pregnancy. In all groups KATP channel blocker glibenclamide antagonized the relaxation of HUV induced by pinacidil without change in the maximal relaxations indicating additional KATP channel-independent mechanisms of pinacidil action. Iberiotoxin, a selective antagonist of large-conductance calcium-activated potassium channels, inhibited the relaxant effect of pinacidil in PIH, but not in normal pregnancy and GDM. Experiments performed in K+-rich solution confirmed the existence of K+-independent effects of pinacidil, which also appear to be impaired in GDM and PIH. Thus, the expression of KATP channels is decreased in GDM and PIH. In GDM, vasorelaxant response of HUV to pinacidil is reduced, while in PIH it remains unchanged. It is very likely that KATP channels modulation and more detailed insight in KATP channel-independent actions of pinacidil may be precious in the therapy of pathological pregnancies. © 2019 Elsevier Inc.
T2  - Experimental and Molecular Pathology
T1  - Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels
VL  - 111
SP  - 104323
DO  - 10.1016/j.yexmp.2019.104323
ER  - 

@article{
author = "Đokić, Vladimir and Janković-Ražnatović, Svetlana and Novaković, Radmila and Kostić, Milan and Rajković, Jovana and Labudović-Borović, Milica and Rakočević, Jelena T. and Stanišić, Jelena and Đurić, Miloš and Gojković-Bukarica, Ljiljana",
year = "2019",
abstract = "Gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) can jeopardize mother and/or fetus. Vascular ATP-sensitive potassium (KATP) channels most likely participate in the processes of diabetes and hypertension. The aim of this research was to examine whether GDM and PIH cause changes in the expression and function of KATP channels in vascular smooth muscle of human umbilical vein (HUV). Western blot and immunohistochemistry detected significantly decreased expression of Kir6.1 subunit of KATP channels in GDM and PIH, while the expression of SUR2B was unchanged. In GDM, a K+ channel opener, pinacidil caused reduced relaxation of the endothelium-denuded HUVs compared to normal pregnancy. However, its effects in HUVs from PIH subjects were similar to normal pregnancy. In all groups KATP channel blocker glibenclamide antagonized the relaxation of HUV induced by pinacidil without change in the maximal relaxations indicating additional KATP channel-independent mechanisms of pinacidil action. Iberiotoxin, a selective antagonist of large-conductance calcium-activated potassium channels, inhibited the relaxant effect of pinacidil in PIH, but not in normal pregnancy and GDM. Experiments performed in K+-rich solution confirmed the existence of K+-independent effects of pinacidil, which also appear to be impaired in GDM and PIH. Thus, the expression of KATP channels is decreased in GDM and PIH. In GDM, vasorelaxant response of HUV to pinacidil is reduced, while in PIH it remains unchanged. It is very likely that KATP channels modulation and more detailed insight in KATP channel-independent actions of pinacidil may be precious in the therapy of pathological pregnancies. © 2019 Elsevier Inc.",
journal = "Experimental and Molecular Pathology",
title = "Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels",
volume = "111",
pages = "104323",
doi = "10.1016/j.yexmp.2019.104323"
}

Đokić, V., Janković-Ražnatović, S., Novaković, R., Kostić, M., Rajković, J., Labudović-Borović, M., Rakočević, J. T., Stanišić, J., Đurić, M.,& Gojković-Bukarica, L.. (2019). Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels. in Experimental and Molecular Pathology, 111, 104323.
https://doi.org/10.1016/j.yexmp.2019.104323

Đokić V, Janković-Ražnatović S, Novaković R, Kostić M, Rajković J, Labudović-Borović M, Rakočević JT, Stanišić J, Đurić M, Gojković-Bukarica L. Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels. in Experimental and Molecular Pathology. 2019;111:104323.
doi:10.1016/j.yexmp.2019.104323 .

Đokić, Vladimir, Janković-Ražnatović, Svetlana, Novaković, Radmila, Kostić, Milan, Rajković, Jovana, Labudović-Borović, Milica, Rakočević, Jelena T., Stanišić, Jelena, Đurić, Miloš, Gojković-Bukarica, Ljiljana, "Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels" in Experimental and Molecular Pathology, 111 (2019):104323,
https://doi.org/10.1016/j.yexmp.2019.104323 . .

TY  - JOUR
AU  - Stojanović, Marija
AU  - Todorović, Dajana D.
AU  - Šćepanović, Ljiljana
AU  - Mitrović, Dušan M.
AU  - Borozan, Sunčica Z.
AU  - Dragutinović, Vesna V.
AU  - Labudović-Borović, Milica
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-018-3311-2
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8060
AB  - The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.
T2  - Molecular and Cellular Biochemistry
T1  - Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue
VL  - 448
IS  - 1-2
SP  - 43
EP  - 50
DO  - 10.1007/s11010-018-3311-2
ER  - 

@article{
author = "Stojanović, Marija and Todorović, Dajana D. and Šćepanović, Ljiljana and Mitrović, Dušan M. and Borozan, Sunčica Z. and Dragutinović, Vesna V. and Labudović-Borović, Milica and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.",
journal = "Molecular and Cellular Biochemistry",
title = "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue",
volume = "448",
number = "1-2",
pages = "43-50",
doi = "10.1007/s11010-018-3311-2"
}

Stojanović, M., Todorović, D. D., Šćepanović, L., Mitrović, D. M., Borozan, S. Z., Dragutinović, V. V., Labudović-Borović, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry, 448(1-2), 43-50.
https://doi.org/10.1007/s11010-018-3311-2

Stojanović M, Todorović DD, Šćepanović L, Mitrović DM, Borozan SZ, Dragutinović VV, Labudović-Borović M, Krstić DZ, Čolović MB, Đurić DM. Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry. 2018;448(1-2):43-50.
doi:10.1007/s11010-018-3311-2 .

Stojanović, Marija, Todorović, Dajana D., Šćepanović, Ljiljana, Mitrović, Dušan M., Borozan, Sunčica Z., Dragutinović, Vesna V., Labudović-Borović, Milica, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue" in Molecular and Cellular Biochemistry, 448, no. 1-2 (2018):43-50,
https://doi.org/10.1007/s11010-018-3311-2 . .

TY  - JOUR
AU  - Petrović, Danijela
AU  - Seke, Mariana
AU  - Labudović-Borović, Milica
AU  - Jović, Danica S.
AU  - Borišev, Ivana
AU  - Srđenović, Branislava U.
AU  - Rakočević, Zlatko Lj.
AU  - Pavlović, Vladimir B.
AU  - Đorđević, Aleksandar N.
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0014480017305890
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7688
AB  - In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p <.05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats.
T2  - Experimental and Molecular Pathology
T1  - Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats
VL  - 104
IS  - 3
SP  - 199
EP  - 211
DO  - 10.1016/j.yexmp.2018.04.005
ER  - 

@article{
author = "Petrović, Danijela and Seke, Mariana and Labudović-Borović, Milica and Jović, Danica S. and Borišev, Ivana and Srđenović, Branislava U. and Rakočević, Zlatko Lj. and Pavlović, Vladimir B. and Đorđević, Aleksandar N.",
year = "2018",
abstract = "In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p <.05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats.",
journal = "Experimental and Molecular Pathology",
title = "Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats",
volume = "104",
number = "3",
pages = "199-211",
doi = "10.1016/j.yexmp.2018.04.005"
}

Petrović, D., Seke, M., Labudović-Borović, M., Jović, D. S., Borišev, I., Srđenović, B. U., Rakočević, Z. Lj., Pavlović, V. B.,& Đorđević, A. N.. (2018). Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats. in Experimental and Molecular Pathology, 104(3), 199-211.
https://doi.org/10.1016/j.yexmp.2018.04.005

Petrović D, Seke M, Labudović-Borović M, Jović DS, Borišev I, Srđenović BU, Rakočević ZL, Pavlović VB, Đorđević AN. Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats. in Experimental and Molecular Pathology. 2018;104(3):199-211.
doi:10.1016/j.yexmp.2018.04.005 .

Petrović, Danijela, Seke, Mariana, Labudović-Borović, Milica, Jović, Danica S., Borišev, Ivana, Srđenović, Branislava U., Rakočević, Zlatko Lj., Pavlović, Vladimir B., Đorđević, Aleksandar N., "Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats" in Experimental and Molecular Pathology, 104, no. 3 (2018):199-211,
https://doi.org/10.1016/j.yexmp.2018.04.005 . .

TY  - CONF
AU  - Rajković, Jovana
AU  - Perić, Miodrag
AU  - Stanišić, Jelena
AU  - Rakočević, Jelena T.
AU  - Novaković, Radmila
AU  - Đokić, Vladimir
AU  - Labudović-Borović, Milica
AU  - Tepavčević, Snežana
AU  - Kanjuh, Vladimir
AU  - Heinle, Helmut
AU  - Gojković-Bukarica, Ljiljana
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018307019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7949
C3  - Atherosclerosis
T1  - Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus
VL  - 275
SP  - e133
DO  - 10.1016/j.atherosclerosis.2018.06.389
ER  - 

@conference{
author = "Rajković, Jovana and Perić, Miodrag and Stanišić, Jelena and Rakočević, Jelena T. and Novaković, Radmila and Đokić, Vladimir and Labudović-Borović, Milica and Tepavčević, Snežana and Kanjuh, Vladimir and Heinle, Helmut and Gojković-Bukarica, Ljiljana",
year = "2018",
journal = "Atherosclerosis",
title = "Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus",
volume = "275",
pages = "e133",
doi = "10.1016/j.atherosclerosis.2018.06.389"
}

Rajković, J., Perić, M., Stanišić, J., Rakočević, J. T., Novaković, R., Đokić, V., Labudović-Borović, M., Tepavčević, S., Kanjuh, V., Heinle, H.,& Gojković-Bukarica, L.. (2018). Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus. in Atherosclerosis, 275, e133.
https://doi.org/10.1016/j.atherosclerosis.2018.06.389

Rajković J, Perić M, Stanišić J, Rakočević JT, Novaković R, Đokić V, Labudović-Borović M, Tepavčević S, Kanjuh V, Heinle H, Gojković-Bukarica L. Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus. in Atherosclerosis. 2018;275:e133.
doi:10.1016/j.atherosclerosis.2018.06.389 .

Rajković, Jovana, Perić, Miodrag, Stanišić, Jelena, Rakočević, Jelena T., Novaković, Radmila, Đokić, Vladimir, Labudović-Borović, Milica, Tepavčević, Snežana, Kanjuh, Vladimir, Heinle, Helmut, Gojković-Bukarica, Ljiljana, "Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus" in Atherosclerosis, 275 (2018):e133,
https://doi.org/10.1016/j.atherosclerosis.2018.06.389 . .

TY  - CONF
AU  - Rajković, Jovana
AU  - Perić, Miodrag
AU  - Stanišić, Jelena
AU  - Rakočević, Jelena T.
AU  - Novaković, Radmila
AU  - Đokić, Vladimir
AU  - Labudović-Borović, Milica
AU  - Tepavčević, Snežana
AU  - Heinle, Helmut
AU  - Gojković-Bukarica, Ljiljana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7912
C3  - European Journal of Clinical Investigation
T1  - Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus
VL  - 48
IS  - SI (Suppl. 1)
SP  - 137 (P118-T)
DO  - 10.1111/eci.12926
ER  - 

@conference{
author = "Rajković, Jovana and Perić, Miodrag and Stanišić, Jelena and Rakočević, Jelena T. and Novaković, Radmila and Đokić, Vladimir and Labudović-Borović, Milica and Tepavčević, Snežana and Heinle, Helmut and Gojković-Bukarica, Ljiljana",
year = "2018",
journal = "European Journal of Clinical Investigation",
title = "Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus",
volume = "48",
number = "SI (Suppl. 1)",
pages = "137 (P118-T)",
doi = "10.1111/eci.12926"
}

Rajković, J., Perić, M., Stanišić, J., Rakočević, J. T., Novaković, R., Đokić, V., Labudović-Borović, M., Tepavčević, S., Heinle, H.,& Gojković-Bukarica, L.. (2018). Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus. in European Journal of Clinical Investigation, 48(SI (Suppl. 1)), 137 (P118-T).
https://doi.org/10.1111/eci.12926

Rajković J, Perić M, Stanišić J, Rakočević JT, Novaković R, Đokić V, Labudović-Borović M, Tepavčević S, Heinle H, Gojković-Bukarica L. Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus. in European Journal of Clinical Investigation. 2018;48(SI (Suppl. 1)):137 (P118-T).
doi:10.1111/eci.12926 .

Rajković, Jovana, Perić, Miodrag, Stanišić, Jelena, Rakočević, Jelena T., Novaković, Radmila, Đokić, Vladimir, Labudović-Borović, Milica, Tepavčević, Snežana, Heinle, Helmut, Gojković-Bukarica, Ljiljana, "Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus" in European Journal of Clinical Investigation, 48, no. SI (Suppl. 1) (2018):137 (P118-T),
https://doi.org/10.1111/eci.12926 . .

TY  - JOUR
AU  - Stanković, D.
AU  - Labudović-Borović, Milica
AU  - Radosavljević, R.
AU  - Marinković, Miloš
AU  - Isenović, Esma R.
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2468785518301071
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7962
AB  - Mucograft is collagen matrix was designed for use in open healing situations due to its compact outer layer. The technique presented in this article is an attempt to avail this attribute for covering open oral wound in guided bone regeneration (GBR) procedure. The essential idea of this technique is to avoid scoring of periosteum, submucosa and muscle layer for buccal flap advancement. Therefore, we used mucograft to cover bone substitute and barrier membrane in GBR surgical procedure. Thus, we avoided periostal-releasing incisions (PRI) and gained reposition of the flap to original level without impairing the attached keratinized gingiva. Buccal flap advancement in situations of shallow vestibulum, shortly attached gingiva and strong muscle pull may reduce or eliminate attached gingiva with an adverse effect on extended survival of placed implants. This technique promises to be beneficial for the preservation of the soft tissue around dental implants after GBR procedure.
T2  - Journal of Stomatology, Oral and Maxillofacial Surgery
T1  - Use of acellular collagen matrix for the closure of the open oral wound in bone regeneration
VL  - 119
IS  - 5
SP  - 446
EP  - 449
DO  - 10.1016/j.jormas.2018.04.015
ER  - 

@article{
author = "Stanković, D. and Labudović-Borović, Milica and Radosavljević, R. and Marinković, Miloš and Isenović, Esma R.",
year = "2018",
abstract = "Mucograft is collagen matrix was designed for use in open healing situations due to its compact outer layer. The technique presented in this article is an attempt to avail this attribute for covering open oral wound in guided bone regeneration (GBR) procedure. The essential idea of this technique is to avoid scoring of periosteum, submucosa and muscle layer for buccal flap advancement. Therefore, we used mucograft to cover bone substitute and barrier membrane in GBR surgical procedure. Thus, we avoided periostal-releasing incisions (PRI) and gained reposition of the flap to original level without impairing the attached keratinized gingiva. Buccal flap advancement in situations of shallow vestibulum, shortly attached gingiva and strong muscle pull may reduce or eliminate attached gingiva with an adverse effect on extended survival of placed implants. This technique promises to be beneficial for the preservation of the soft tissue around dental implants after GBR procedure.",
journal = "Journal of Stomatology, Oral and Maxillofacial Surgery",
title = "Use of acellular collagen matrix for the closure of the open oral wound in bone regeneration",
volume = "119",
number = "5",
pages = "446-449",
doi = "10.1016/j.jormas.2018.04.015"
}

Stanković, D., Labudović-Borović, M., Radosavljević, R., Marinković, M.,& Isenović, E. R.. (2018). Use of acellular collagen matrix for the closure of the open oral wound in bone regeneration. in Journal of Stomatology, Oral and Maxillofacial Surgery, 119(5), 446-449.
https://doi.org/10.1016/j.jormas.2018.04.015

Stanković D, Labudović-Borović M, Radosavljević R, Marinković M, Isenović ER. Use of acellular collagen matrix for the closure of the open oral wound in bone regeneration. in Journal of Stomatology, Oral and Maxillofacial Surgery. 2018;119(5):446-449.
doi:10.1016/j.jormas.2018.04.015 .

Stanković, D., Labudović-Borović, Milica, Radosavljević, R., Marinković, Miloš, Isenović, Esma R., "Use of acellular collagen matrix for the closure of the open oral wound in bone regeneration" in Journal of Stomatology, Oral and Maxillofacial Surgery, 119, no. 5 (2018):446-449,
https://doi.org/10.1016/j.jormas.2018.04.015 . .

TY  - CONF
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović-Borović, Milica
AU  - Jović, Danica S.
AU  - Borišev, Ivana
AU  - Kanacki, Zdenko
AU  - Zikić, Dragan
AU  - Đorđević, Aleksandar N.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7182
AB  - Background: Doxorubicin is a first line cancer chemotherapeutic. Unfortunately, its clinical use is limited by its cardiotoxicity. It is known that iron overload aggravates anthracycline toxicity. Fullerenol is a 1 nm size molecule and in aqueous solutions is in the form of polyanionic nanoparticles, which enables them to serve as a good carrier of positively charged ions such as Fe2+. Fullerenol’s antioxidant activity through scavenging free radicals has already been proved in different biological systems.
Methods: The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite as a pretreatment to doxorubicin on the rat’s heart in comparison to doxorubicin alone. After the 24h-treatment, adult male Wistar rats were sacrificed and hearts were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, we had chosen to monitor gene expression of enzymes involved in antioxidant defense.
Results: Ultrastructural study revealed that in the group pretreated with the nanocomposite prior to doxorubicin application cardiomyocytes were with preserved morphology and the structure of intercalated discs. On the other hand, the heart tissues of animals treated with doxorubicin alone were significantly more damaged. Intensive interstitial edema was observed, as well as vacuolization of cardiomyocytes, hypercontraction of sarcomeres, mitochondria of irregular shapes. qRT-PCR results have shown that neither treatment with doxorubicin alone nor the pretreatment with the nanocomposite did cause significant increase in mRNA levels of catalase and superoxide dismutase.
Conclusions: Our results indicate that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin induces less damage to the hearth tissue in comparison to doxorubicin alone.
C3  - Annals of Oncology
T1  - Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity
VL  - 28
IS  - Supplement 5
DO  - 10.1093/annonc/mdx390.057
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7182
ER  - 

@conference{
author = "Seke, Mariana and Petrović, Danijela and Labudović-Borović, Milica and Jović, Danica S. and Borišev, Ivana and Kanacki, Zdenko and Zikić, Dragan and Đorđević, Aleksandar N.",
year = "2017",
abstract = "Background: Doxorubicin is a first line cancer chemotherapeutic. Unfortunately, its clinical use is limited by its cardiotoxicity. It is known that iron overload aggravates anthracycline toxicity. Fullerenol is a 1 nm size molecule and in aqueous solutions is in the form of polyanionic nanoparticles, which enables them to serve as a good carrier of positively charged ions such as Fe2+. Fullerenol’s antioxidant activity through scavenging free radicals has already been proved in different biological systems.
Methods: The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite as a pretreatment to doxorubicin on the rat’s heart in comparison to doxorubicin alone. After the 24h-treatment, adult male Wistar rats were sacrificed and hearts were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, we had chosen to monitor gene expression of enzymes involved in antioxidant defense.
Results: Ultrastructural study revealed that in the group pretreated with the nanocomposite prior to doxorubicin application cardiomyocytes were with preserved morphology and the structure of intercalated discs. On the other hand, the heart tissues of animals treated with doxorubicin alone were significantly more damaged. Intensive interstitial edema was observed, as well as vacuolization of cardiomyocytes, hypercontraction of sarcomeres, mitochondria of irregular shapes. qRT-PCR results have shown that neither treatment with doxorubicin alone nor the pretreatment with the nanocomposite did cause significant increase in mRNA levels of catalase and superoxide dismutase.
Conclusions: Our results indicate that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin induces less damage to the hearth tissue in comparison to doxorubicin alone.",
journal = "Annals of Oncology",
title = "Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity",
volume = "28",
number = "Supplement 5",
doi = "10.1093/annonc/mdx390.057",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7182"
}

Seke, M., Petrović, D., Labudović-Borović, M., Jović, D. S., Borišev, I., Kanacki, Z., Zikić, D.,& Đorđević, A. N.. (2017). Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity. in Annals of Oncology, 28(Supplement 5).
https://doi.org/10.1093/annonc/mdx390.057
https://hdl.handle.net/21.15107/rcub_vinar_7182

Seke M, Petrović D, Labudović-Borović M, Jović DS, Borišev I, Kanacki Z, Zikić D, Đorđević AN. Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity. in Annals of Oncology. 2017;28(Supplement 5).
doi:10.1093/annonc/mdx390.057
https://hdl.handle.net/21.15107/rcub_vinar_7182 .

Seke, Mariana, Petrović, Danijela, Labudović-Borović, Milica, Jović, Danica S., Borišev, Ivana, Kanacki, Zdenko, Zikić, Dragan, Đorđević, Aleksandar N., "Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity" in Annals of Oncology, 28, no. Supplement 5 (2017),
https://doi.org/10.1093/annonc/mdx390.057 .,
https://hdl.handle.net/21.15107/rcub_vinar_7182 .

TY  - CONF
AU  - Rajković, Jovana
AU  - Perić, Miodrag
AU  - Stanišić, Jelena
AU  - Rakočević, Jelena T.
AU  - Novaković, Radmila
AU  - Đokić, Vladimir
AU  - Labudović-Borović, Milica
AU  - Tepavčević, Snežana
AU  - Živanović, Vladimir
AU  - Kanjuh, Vladimir
AU  - Heinle, Helmut
AU  - Gojković-Bukarica, Ljiljana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7174
C3  - Atherosclerosis
T1  - Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus
VL  - 263
SP  - E131
EP  - E131
DO  - 10.1016/j.atherosclerosis.2017.06.418
ER  - 

@conference{
author = "Rajković, Jovana and Perić, Miodrag and Stanišić, Jelena and Rakočević, Jelena T. and Novaković, Radmila and Đokić, Vladimir and Labudović-Borović, Milica and Tepavčević, Snežana and Živanović, Vladimir and Kanjuh, Vladimir and Heinle, Helmut and Gojković-Bukarica, Ljiljana",
year = "2017",
journal = "Atherosclerosis",
title = "Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus",
volume = "263",
pages = "E131-E131",
doi = "10.1016/j.atherosclerosis.2017.06.418"
}

Rajković, J., Perić, M., Stanišić, J., Rakočević, J. T., Novaković, R., Đokić, V., Labudović-Borović, M., Tepavčević, S., Živanović, V., Kanjuh, V., Heinle, H.,& Gojković-Bukarica, L.. (2017). Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus. in Atherosclerosis, 263, E131-E131.
https://doi.org/10.1016/j.atherosclerosis.2017.06.418

Rajković J, Perić M, Stanišić J, Rakočević JT, Novaković R, Đokić V, Labudović-Borović M, Tepavčević S, Živanović V, Kanjuh V, Heinle H, Gojković-Bukarica L. Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus. in Atherosclerosis. 2017;263:E131-E131.
doi:10.1016/j.atherosclerosis.2017.06.418 .

Rajković, Jovana, Perić, Miodrag, Stanišić, Jelena, Rakočević, Jelena T., Novaković, Radmila, Đokić, Vladimir, Labudović-Borović, Milica, Tepavčević, Snežana, Živanović, Vladimir, Kanjuh, Vladimir, Heinle, Helmut, Gojković-Bukarica, Ljiljana, "Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus" in Atherosclerosis, 263 (2017):E131-E131,
https://doi.org/10.1016/j.atherosclerosis.2017.06.418 . .

TY  - JOUR
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Đorđević, Aleksandar N.
AU  - Jović, Danica S.
AU  - Labudović-Borović, Milica
AU  - Kanački, Zdenko
AU  - Janković, Milan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1306
AB  - Fullerenol (C-60(OH)(24)) is present in aqueous solutions in the form of polyanion nanoparticles with particles size distribution within the range from 15 to 42 nm. In this research it is assumed that these features could enable fullerenol nanoparticles (FNPs) to bind positively charged molecules like doxorubicin (DOX) and serve as drug carriers. Considering this, fullerenol/doxorubicin nanocomposite (FNP/DOX) is formed and characterized by ultra-performance liquid chromatography tandem mass spectrometry, dynamic light scattering, atomic force microscopy and transmission electron microscopy. Measurements have shown that DOX did not significantly affect particle size (23 nm). It is also assumed that FNP/DOX could reduce the acute cardiotoxic effects of DOX in vivo (Wistar rats treated i.p.). In this study, quantitative real time polymerase chain reaction results have shown that treatment with DOX alone caused significant increase in mRNA levels of catalase (p LT 0.05) enzyme indicating the presence of oxidative stress. This effect is significantly reduced by the treatment with FNP/DOX (p LT 0.05). Furthermore, mRNA levels of antiapoptotic enzyme (Bcl-2) are significantly increased (p LT 0.05) in all treated groups, particularly where FNP/DOX was applied, suggesting cell resistance to apoptosis. Moreover, ultrastructural analysis has shown the absence of myelin figures within the mitochondria in the heart tissue with FNP/DOX treatment, indicating reduction of oxidative stress. Hence, our results have implied that FNP/DOX is generally less harmful to the heart compared to DOX.
T2  - Nanotechnology
T1  - Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue
VL  - 27
IS  - 48
DO  - 10.1088/0957-4484/27/48/485101
ER  - 

@article{
author = "Seke, Mariana and Petrović, Danijela and Đorđević, Aleksandar N. and Jović, Danica S. and Labudović-Borović, Milica and Kanački, Zdenko and Janković, Milan",
year = "2016",
abstract = "Fullerenol (C-60(OH)(24)) is present in aqueous solutions in the form of polyanion nanoparticles with particles size distribution within the range from 15 to 42 nm. In this research it is assumed that these features could enable fullerenol nanoparticles (FNPs) to bind positively charged molecules like doxorubicin (DOX) and serve as drug carriers. Considering this, fullerenol/doxorubicin nanocomposite (FNP/DOX) is formed and characterized by ultra-performance liquid chromatography tandem mass spectrometry, dynamic light scattering, atomic force microscopy and transmission electron microscopy. Measurements have shown that DOX did not significantly affect particle size (23 nm). It is also assumed that FNP/DOX could reduce the acute cardiotoxic effects of DOX in vivo (Wistar rats treated i.p.). In this study, quantitative real time polymerase chain reaction results have shown that treatment with DOX alone caused significant increase in mRNA levels of catalase (p LT 0.05) enzyme indicating the presence of oxidative stress. This effect is significantly reduced by the treatment with FNP/DOX (p LT 0.05). Furthermore, mRNA levels of antiapoptotic enzyme (Bcl-2) are significantly increased (p LT 0.05) in all treated groups, particularly where FNP/DOX was applied, suggesting cell resistance to apoptosis. Moreover, ultrastructural analysis has shown the absence of myelin figures within the mitochondria in the heart tissue with FNP/DOX treatment, indicating reduction of oxidative stress. Hence, our results have implied that FNP/DOX is generally less harmful to the heart compared to DOX.",
journal = "Nanotechnology",
title = "Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue",
volume = "27",
number = "48",
doi = "10.1088/0957-4484/27/48/485101"
}

Seke, M., Petrović, D., Đorđević, A. N., Jović, D. S., Labudović-Borović, M., Kanački, Z.,& Janković, M.. (2016). Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue. in Nanotechnology, 27(48).
https://doi.org/10.1088/0957-4484/27/48/485101

Seke M, Petrović D, Đorđević AN, Jović DS, Labudović-Borović M, Kanački Z, Janković M. Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue. in Nanotechnology. 2016;27(48).
doi:10.1088/0957-4484/27/48/485101 .

Seke, Mariana, Petrović, Danijela, Đorđević, Aleksandar N., Jović, Danica S., Labudović-Borović, Milica, Kanački, Zdenko, Janković, Milan, "Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue" in Nanotechnology, 27, no. 48 (2016),
https://doi.org/10.1088/0957-4484/27/48/485101 . .

TY  - CONF
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović-Borović, Milica
AU  - Jović, Danica S.
AU  - Srđenović, Branislava U.
AU  - Kanacki, Zdenko
AU  - Zikić, Dragan
AU  - Đorđević, Aleksandar N.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7081
C3  - FEBS Journal
T1  - Effects of a fullerenol/doxorubicin nanocomposite on the heart tissue of healthy rats
VL  - 282
SP  - 242
EP  - 242
DO  - 10.1111/febs.13339
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7081
ER  - 

@conference{
author = "Seke, Mariana and Petrović, Danijela and Labudović-Borović, Milica and Jović, Danica S. and Srđenović, Branislava U. and Kanacki, Zdenko and Zikić, Dragan and Đorđević, Aleksandar N.",
year = "2015",
journal = "FEBS Journal",
title = "Effects of a fullerenol/doxorubicin nanocomposite on the heart tissue of healthy rats",
volume = "282",
pages = "242-242",
doi = "10.1111/febs.13339",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7081"
}

Seke, M., Petrović, D., Labudović-Borović, M., Jović, D. S., Srđenović, B. U., Kanacki, Z., Zikić, D.,& Đorđević, A. N.. (2015). Effects of a fullerenol/doxorubicin nanocomposite on the heart tissue of healthy rats. in FEBS Journal, 282, 242-242.
https://doi.org/10.1111/febs.13339
https://hdl.handle.net/21.15107/rcub_vinar_7081

Seke M, Petrović D, Labudović-Borović M, Jović DS, Srđenović BU, Kanacki Z, Zikić D, Đorđević AN. Effects of a fullerenol/doxorubicin nanocomposite on the heart tissue of healthy rats. in FEBS Journal. 2015;282:242-242.
doi:10.1111/febs.13339
https://hdl.handle.net/21.15107/rcub_vinar_7081 .

Seke, Mariana, Petrović, Danijela, Labudović-Borović, Milica, Jović, Danica S., Srđenović, Branislava U., Kanacki, Zdenko, Zikić, Dragan, Đorđević, Aleksandar N., "Effects of a fullerenol/doxorubicin nanocomposite on the heart tissue of healthy rats" in FEBS Journal, 282 (2015):242-242,
https://doi.org/10.1111/febs.13339 .,
https://hdl.handle.net/21.15107/rcub_vinar_7081 .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Sudar, Emina
AU  - Zafirović, Sonja
AU  - Stanimirović, Julijana
AU  - Labudović-Borović, Milica
AU  - Isenović, Esma R.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/286
AB  - We studied the in vivo effects of estradiol on size and biochemical parameters of cardiomyocytes in pathophysiological conditions such as obesity and insulin resistance. Male Wistar rats were normally fed (controls, n = 7) or fed with high-fat diet (obese, n = 14). Half of the obese rats (obese + estradiol, n = 7) were treated with a single dose of estradiol (40 g/kg, intraperitoneally) and 24 hours after treatment all the rats were killed. Estradiol in vivo in obese rats resulted in a significant increase in protein kinase B (Akt) activation (P LT .05) and decrease in heart mass (P LT .05), ratio of the heart mass/body mass (P LT .05), transverse diameters of cardiomyocytes (P LT .001), concentration of serum high-sensitivity C-reactive protein (P LT .001), and total cholesterol (P LT .01) compared with obese nontreated rats. Our results suggest that estradiol in obese/IR rats affects the size of cardiomyocytes and its actions lead in vivo to a reduction in obesity-induced cardiac hypertrophy, via Akt.
T2  - Angiology
T1  - Estradiol In Vivo Induces Changes in Cardiomyocytes Size in Obese Rats
VL  - 66
IS  - 1
SP  - 25
EP  - 35
DO  - 10.1177/0003319713514477
ER  - 

@article{
author = "Obradović, Milan M. and Sudar, Emina and Zafirović, Sonja and Stanimirović, Julijana and Labudović-Borović, Milica and Isenović, Esma R.",
year = "2015",
abstract = "We studied the in vivo effects of estradiol on size and biochemical parameters of cardiomyocytes in pathophysiological conditions such as obesity and insulin resistance. Male Wistar rats were normally fed (controls, n = 7) or fed with high-fat diet (obese, n = 14). Half of the obese rats (obese + estradiol, n = 7) were treated with a single dose of estradiol (40 g/kg, intraperitoneally) and 24 hours after treatment all the rats were killed. Estradiol in vivo in obese rats resulted in a significant increase in protein kinase B (Akt) activation (P LT .05) and decrease in heart mass (P LT .05), ratio of the heart mass/body mass (P LT .05), transverse diameters of cardiomyocytes (P LT .001), concentration of serum high-sensitivity C-reactive protein (P LT .001), and total cholesterol (P LT .01) compared with obese nontreated rats. Our results suggest that estradiol in obese/IR rats affects the size of cardiomyocytes and its actions lead in vivo to a reduction in obesity-induced cardiac hypertrophy, via Akt.",
journal = "Angiology",
title = "Estradiol In Vivo Induces Changes in Cardiomyocytes Size in Obese Rats",
volume = "66",
number = "1",
pages = "25-35",
doi = "10.1177/0003319713514477"
}

Obradović, M. M., Sudar, E., Zafirović, S., Stanimirović, J., Labudović-Borović, M.,& Isenović, E. R.. (2015). Estradiol In Vivo Induces Changes in Cardiomyocytes Size in Obese Rats. in Angiology, 66(1), 25-35.
https://doi.org/10.1177/0003319713514477

Obradović MM, Sudar E, Zafirović S, Stanimirović J, Labudović-Borović M, Isenović ER. Estradiol In Vivo Induces Changes in Cardiomyocytes Size in Obese Rats. in Angiology. 2015;66(1):25-35.
doi:10.1177/0003319713514477 .

Obradović, Milan M., Sudar, Emina, Zafirović, Sonja, Stanimirović, Julijana, Labudović-Borović, Milica, Isenović, Esma R., "Estradiol In Vivo Induces Changes in Cardiomyocytes Size in Obese Rats" in Angiology, 66, no. 1 (2015):25-35,
https://doi.org/10.1177/0003319713514477 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Jovanović, Aleksandra
AU  - Sudar, Emina
AU  - Mousa, Shaker A.
AU  - Labudović-Borović, Milica
AU  - Isenović, Esma R.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/803
AB  - The aim of this study was to investigate in vivo effects of estradiol on Na+/K+-ATPase activity/expression in high fat (HF) diet fed rats. Adult male Wistar rats were fed normally (Control, n = 7) or with a HF diet (Obese, n = 14) for 10 weeks. After 10 weeks, half of the obese rats were treated with estradiol (Obese + Estradiol, n = 7, 40 mu g/kg, i.p.) as a bolus injection and 24 h after treatment all the rats were sacrificed. Estradiol in vivo in obese rats in comparison with obese non-treated rats led to a statistically significant increase in concentration of serum Na+ (p LT 0.05), Na+/K+-ATPase activity (p LT 0.01), expression of alpha 1 (p LT 0.01) and alpha 2 (p LT 0.05) subunit of Na+/K+-ATPase, both PI3K subunits p85 (p LT 0.01), p110 (p LT 0.05), and association of IRS-1 with p85 (p LT 0.05), while significantly decrease expression of AT1 (p LT 0.05) and Rho A (p LT 0.01) proteins. Our results suggest that estradiol in vivo in pathophysiological conditions, such as obesity accompanied with insulin resistance stimulates activity and expression of Na+/K+-ATPase by a mechanism that involves the participation of IRS-1/PI3K/Akt signaling. In addition, the decreasing level of AT1 and Rho A proteins estradiol probably attenuates the detrimental effect of obesity to decreased IRS-1/P13K association and consequently reduce Na+/K+-ATPase activity/expression. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Molecular and Cellular Endocrinology
T1  - Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats
VL  - 416
IS  - C
SP  - 46
EP  - 56
DO  - 10.1016/j.mce.2015.08.020
ER  - 

@article{
author = "Obradović, Milan M. and Zafirović, Sonja and Jovanović, Aleksandra and Sudar, Emina and Mousa, Shaker A. and Labudović-Borović, Milica and Isenović, Esma R.",
year = "2015",
abstract = "The aim of this study was to investigate in vivo effects of estradiol on Na+/K+-ATPase activity/expression in high fat (HF) diet fed rats. Adult male Wistar rats were fed normally (Control, n = 7) or with a HF diet (Obese, n = 14) for 10 weeks. After 10 weeks, half of the obese rats were treated with estradiol (Obese + Estradiol, n = 7, 40 mu g/kg, i.p.) as a bolus injection and 24 h after treatment all the rats were sacrificed. Estradiol in vivo in obese rats in comparison with obese non-treated rats led to a statistically significant increase in concentration of serum Na+ (p LT 0.05), Na+/K+-ATPase activity (p LT 0.01), expression of alpha 1 (p LT 0.01) and alpha 2 (p LT 0.05) subunit of Na+/K+-ATPase, both PI3K subunits p85 (p LT 0.01), p110 (p LT 0.05), and association of IRS-1 with p85 (p LT 0.05), while significantly decrease expression of AT1 (p LT 0.05) and Rho A (p LT 0.01) proteins. Our results suggest that estradiol in vivo in pathophysiological conditions, such as obesity accompanied with insulin resistance stimulates activity and expression of Na+/K+-ATPase by a mechanism that involves the participation of IRS-1/PI3K/Akt signaling. In addition, the decreasing level of AT1 and Rho A proteins estradiol probably attenuates the detrimental effect of obesity to decreased IRS-1/P13K association and consequently reduce Na+/K+-ATPase activity/expression. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Molecular and Cellular Endocrinology",
title = "Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats",
volume = "416",
number = "C",
pages = "46-56",
doi = "10.1016/j.mce.2015.08.020"
}

Obradović, M. M., Zafirović, S., Jovanović, A., Sudar, E., Mousa, S. A., Labudović-Borović, M.,& Isenović, E. R.. (2015). Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats. in Molecular and Cellular Endocrinology
Elsevier., 416(C), 46-56.
https://doi.org/10.1016/j.mce.2015.08.020

Obradović MM, Zafirović S, Jovanović A, Sudar E, Mousa SA, Labudović-Borović M, Isenović ER. Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats. in Molecular and Cellular Endocrinology. 2015;416(C):46-56.
doi:10.1016/j.mce.2015.08.020 .

Obradović, Milan M., Zafirović, Sonja, Jovanović, Aleksandra, Sudar, Emina, Mousa, Shaker A., Labudović-Borović, Milica, Isenović, Esma R., "Effects of 17 beta-estradiol on cardiac Na+/K+-ATPase in high fat diet fed rats" in Molecular and Cellular Endocrinology, 416, no. C (2015):46-56,
https://doi.org/10.1016/j.mce.2015.08.020 . .

TY  - JOUR
AU  - Labudović-Borović, Milica
AU  - Icevic, Ivana
AU  - Kanački, Zdenko
AU  - Zikic, Dragan
AU  - Seke, Mariana
AU  - Injac, Rade
AU  - Đorđević, Aleksandar N.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5924
AB  - Cardioprotective effects of fullerenol C-60(OH)(24) nanoparticles (FNP) were investigated in pigs after a single treatment with doxorubicin (DOX). Semithin and ultrathin sections of myocardial tissue routinely prepared for transmission electron microscopy were analyzed. Extensive intracellular damage was confirmed in cardiomyocytes of DOX-treated animals. By means of ultrastructural analysis, a certain degree of parenchymal degeneration was confirmed even in animals treated with FNP alone, including both the oral and the intraperitoneal application of the substance. The cardioprotective effects of FNP in animals previously treated with DOX were recognized to a certain extent, but were not fully confirmed at the ultrastructural level. Nevertheless, the myocardial morphology of DOX-treated animals improved after the admission of FNP. Irregular orientation of myofibrils, myofibrillar disruption, intracellular edema, and vacuolization were reduced, but not completely eliminated. Reduction of these cellular alterations was achieved if FNP was applied orally 6 h prior to DOX treatment in a dose of 18 mg/kg. However, numerous defects, including the inner mitochondrial membrane and the plasma membrane disruption of certain cells persisted. In FNP/DOX-treated animals, the presence of multinuclear cells with mitosis-like figures resembling metaphase or anaphase were observed, indicating that DOX and FNP could have a complex influence on the cell cycle of cardiomyocytes. Based on this experiment, further careful increase in dosage may be advised to enhance FNP-induced cardioprotection. These investigations should, however, always be combined with ultrastructural analysis. The FNP/DOX interaction is an excellent model for the investigation of cardiomyocyte cell death and cell cycle mechanisms.
T2  - Ultrastructural Pathology
T1  - Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study
VL  - 38
IS  - 2
SP  - 150
EP  - 163
DO  - 10.3109/01913123.2013.822045
ER  - 

@article{
author = "Labudović-Borović, Milica and Icevic, Ivana and Kanački, Zdenko and Zikic, Dragan and Seke, Mariana and Injac, Rade and Đorđević, Aleksandar N.",
year = "2014",
abstract = "Cardioprotective effects of fullerenol C-60(OH)(24) nanoparticles (FNP) were investigated in pigs after a single treatment with doxorubicin (DOX). Semithin and ultrathin sections of myocardial tissue routinely prepared for transmission electron microscopy were analyzed. Extensive intracellular damage was confirmed in cardiomyocytes of DOX-treated animals. By means of ultrastructural analysis, a certain degree of parenchymal degeneration was confirmed even in animals treated with FNP alone, including both the oral and the intraperitoneal application of the substance. The cardioprotective effects of FNP in animals previously treated with DOX were recognized to a certain extent, but were not fully confirmed at the ultrastructural level. Nevertheless, the myocardial morphology of DOX-treated animals improved after the admission of FNP. Irregular orientation of myofibrils, myofibrillar disruption, intracellular edema, and vacuolization were reduced, but not completely eliminated. Reduction of these cellular alterations was achieved if FNP was applied orally 6 h prior to DOX treatment in a dose of 18 mg/kg. However, numerous defects, including the inner mitochondrial membrane and the plasma membrane disruption of certain cells persisted. In FNP/DOX-treated animals, the presence of multinuclear cells with mitosis-like figures resembling metaphase or anaphase were observed, indicating that DOX and FNP could have a complex influence on the cell cycle of cardiomyocytes. Based on this experiment, further careful increase in dosage may be advised to enhance FNP-induced cardioprotection. These investigations should, however, always be combined with ultrastructural analysis. The FNP/DOX interaction is an excellent model for the investigation of cardiomyocyte cell death and cell cycle mechanisms.",
journal = "Ultrastructural Pathology",
title = "Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study",
volume = "38",
number = "2",
pages = "150-163",
doi = "10.3109/01913123.2013.822045"
}

Labudović-Borović, M., Icevic, I., Kanački, Z., Zikic, D., Seke, M., Injac, R.,& Đorđević, A. N.. (2014). Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study. in Ultrastructural Pathology, 38(2), 150-163.
https://doi.org/10.3109/01913123.2013.822045

Labudović-Borović M, Icevic I, Kanački Z, Zikic D, Seke M, Injac R, Đorđević AN. Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study. in Ultrastructural Pathology. 2014;38(2):150-163.
doi:10.3109/01913123.2013.822045 .

Labudović-Borović, Milica, Icevic, Ivana, Kanački, Zdenko, Zikic, Dragan, Seke, Mariana, Injac, Rade, Đorđević, Aleksandar N., "Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study" in Ultrastructural Pathology, 38, no. 2 (2014):150-163,
https://doi.org/10.3109/01913123.2013.822045 . .

TY  - JOUR
AU  - Rizzo, Manfredi
AU  - Obradović, Milan M.
AU  - Labudović-Borović, Milica
AU  - Nikolić, Dragana
AU  - Montalto, Giuseppe
AU  - Rizvi, Ali A.
AU  - Mikhailidis, Dimitri P.
AU  - Isenović, Esma R.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/290
AB  - In humans uric acid (UA) is the end product of degradation of purines. The handling of UA by the renal system is a complex process which is not fully understood. To date, several urate transporters in the renal proximal tubule have been identified. Among them, urate transporter 1 (URAT1) and a glucose transporter 9 (GLUT9) are considered of greater importance, as potential targets for treatment of hyperuricemia and the potential associated cardio-metabolic risk. Therefore, the recognition of the metabolic pathway of UA and elucidation of occurrence of hyperuricemia may provide important insights about the relationship between UA, pre-hypertension (preHT) and the metabolic syndrome (MetS). We also review the available clinical studies in this field, including experimental studies dealing with the mechanisms of UA transport via different transporters, as well as current treatment options for hyperuricemia in patients with MetS, preHT or cardiovascular risk factors.
T2  - Current Vascular Pharmacology
T1  - Uric Acid Metabolism in Pre-hypertension and the Metabolic Syndrome
VL  - 12
IS  - 4
SP  - 572
EP  - 585
DO  - 10.2174/1570161111999131205160756
ER  - 

@article{
author = "Rizzo, Manfredi and Obradović, Milan M. and Labudović-Borović, Milica and Nikolić, Dragana and Montalto, Giuseppe and Rizvi, Ali A. and Mikhailidis, Dimitri P. and Isenović, Esma R.",
year = "2014",
abstract = "In humans uric acid (UA) is the end product of degradation of purines. The handling of UA by the renal system is a complex process which is not fully understood. To date, several urate transporters in the renal proximal tubule have been identified. Among them, urate transporter 1 (URAT1) and a glucose transporter 9 (GLUT9) are considered of greater importance, as potential targets for treatment of hyperuricemia and the potential associated cardio-metabolic risk. Therefore, the recognition of the metabolic pathway of UA and elucidation of occurrence of hyperuricemia may provide important insights about the relationship between UA, pre-hypertension (preHT) and the metabolic syndrome (MetS). We also review the available clinical studies in this field, including experimental studies dealing with the mechanisms of UA transport via different transporters, as well as current treatment options for hyperuricemia in patients with MetS, preHT or cardiovascular risk factors.",
journal = "Current Vascular Pharmacology",
title = "Uric Acid Metabolism in Pre-hypertension and the Metabolic Syndrome",
volume = "12",
number = "4",
pages = "572-585",
doi = "10.2174/1570161111999131205160756"
}

Rizzo, M., Obradović, M. M., Labudović-Borović, M., Nikolić, D., Montalto, G., Rizvi, A. A., Mikhailidis, D. P.,& Isenović, E. R.. (2014). Uric Acid Metabolism in Pre-hypertension and the Metabolic Syndrome. in Current Vascular Pharmacology, 12(4), 572-585.
https://doi.org/10.2174/1570161111999131205160756

Rizzo M, Obradović MM, Labudović-Borović M, Nikolić D, Montalto G, Rizvi AA, Mikhailidis DP, Isenović ER. Uric Acid Metabolism in Pre-hypertension and the Metabolic Syndrome. in Current Vascular Pharmacology. 2014;12(4):572-585.
doi:10.2174/1570161111999131205160756 .

Rizzo, Manfredi, Obradović, Milan M., Labudović-Borović, Milica, Nikolić, Dragana, Montalto, Giuseppe, Rizvi, Ali A., Mikhailidis, Dimitri P., Isenović, Esma R., "Uric Acid Metabolism in Pre-hypertension and the Metabolic Syndrome" in Current Vascular Pharmacology, 12, no. 4 (2014):572-585,
https://doi.org/10.2174/1570161111999131205160756 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Stewart, Alan J.
AU  - Pitt, Samantha J.
AU  - Labudović-Borović, Milica
AU  - Sudar, Emina
AU  - Petrovic, Voin
AU  - Zafirović, Sonja
AU  - Maravić-Stojković, Vera
AU  - Vasić, Vesna M.
AU  - Isenović, Esma R.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5989
AB  - In this study the in vivo effects of estradiol in regulating Na+/K+-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 mu g/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na+/K(+)ATPase activity, expression of the alpha l subunit, and phosphorylation of the al subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na+ levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na+/K+-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na+/K+-ATPase expression and activity in rat heart. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
T2  - Molecular and Cellular Endocrinology
T1  - In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart
VL  - 388
IS  - 1-2
SP  - 58
EP  - 68
DO  - 10.1016/j.mce.2014.03.005
ER  - 

@article{
author = "Obradović, Milan M. and Stewart, Alan J. and Pitt, Samantha J. and Labudović-Borović, Milica and Sudar, Emina and Petrovic, Voin and Zafirović, Sonja and Maravić-Stojković, Vera and Vasić, Vesna M. and Isenović, Esma R.",
year = "2014",
abstract = "In this study the in vivo effects of estradiol in regulating Na+/K+-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 mu g/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na+/K(+)ATPase activity, expression of the alpha l subunit, and phosphorylation of the al subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na+ levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na+/K+-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na+/K+-ATPase expression and activity in rat heart. (C) 2014 Elsevier Ireland Ltd. All rights reserved.",
journal = "Molecular and Cellular Endocrinology",
title = "In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart",
volume = "388",
number = "1-2",
pages = "58-68",
doi = "10.1016/j.mce.2014.03.005"
}

Obradović, M. M., Stewart, A. J., Pitt, S. J., Labudović-Borović, M., Sudar, E., Petrovic, V., Zafirović, S., Maravić-Stojković, V., Vasić, V. M.,& Isenović, E. R.. (2014). In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart. in Molecular and Cellular Endocrinology, 388(1-2), 58-68.
https://doi.org/10.1016/j.mce.2014.03.005

Obradović MM, Stewart AJ, Pitt SJ, Labudović-Borović M, Sudar E, Petrovic V, Zafirović S, Maravić-Stojković V, Vasić VM, Isenović ER. In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart. in Molecular and Cellular Endocrinology. 2014;388(1-2):58-68.
doi:10.1016/j.mce.2014.03.005 .

Obradović, Milan M., Stewart, Alan J., Pitt, Samantha J., Labudović-Borović, Milica, Sudar, Emina, Petrovic, Voin, Zafirović, Sonja, Maravić-Stojković, Vera, Vasić, Vesna M., Isenović, Esma R., "In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart" in Molecular and Cellular Endocrinology, 388, no. 1-2 (2014):58-68,
https://doi.org/10.1016/j.mce.2014.03.005 . .