TY  - JOUR
AU  - Tanić, Nikola
AU  - Stanojević, Boban
AU  - Tanić, Nikola
AU  - Schaefer, Stephan
AU  - Niesters, Hubert G. M.
AU  - Božić, Milena
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3789
AB  - Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Journal of Virological Methods
T1  - Concurrent quantitation of the A and D genotypes of hepatitis B virus
VL  - 161
IS  - 2
SP  - 265
EP  - 270
DO  - 10.1016/j.jviromet.2009.06.022
ER  - 

@article{
author = "Tanić, Nikola and Stanojević, Boban and Tanić, Nikola and Schaefer, Stephan and Niesters, Hubert G. M. and Božić, Milena and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Journal of Virological Methods",
title = "Concurrent quantitation of the A and D genotypes of hepatitis B virus",
volume = "161",
number = "2",
pages = "265-270",
doi = "10.1016/j.jviromet.2009.06.022"
}

Tanić, N., Stanojević, B., Tanić, N., Schaefer, S., Niesters, H. G. M., Božić, M.,& Dimitrijević, B. B.. (2009). Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods, 161(2), 265-270.
https://doi.org/10.1016/j.jviromet.2009.06.022

Tanić N, Stanojević B, Tanić N, Schaefer S, Niesters HGM, Božić M, Dimitrijević BB. Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods. 2009;161(2):265-270.
doi:10.1016/j.jviromet.2009.06.022 .

Tanić, Nikola, Stanojević, Boban, Tanić, Nikola, Schaefer, Stephan, Niesters, Hubert G. M., Božić, Milena, Dimitrijević, Bogomir B., "Concurrent quantitation of the A and D genotypes of hepatitis B virus" in Journal of Virological Methods, 161, no. 2 (2009):265-270,
https://doi.org/10.1016/j.jviromet.2009.06.022 . .

TY  - JOUR
AU  - Jovanović-Ćupić, Snežana P.
AU  - Simonovic-Babic, Jasmina
AU  - Blagojevic, Jelena
AU  - Božić, Milena
AU  - Ješić, Rada
AU  - Nozic, D.
AU  - Stamenković, Gorana
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3752
AB  - Different types of interferon are widely used to treat hepatitis C virus (HCV) infection. Results obtained in vitro suggest that interferon inhibits internal ribosome entry site (IRES)-mediated translation of the HCV genome. To elucidate the possible effect of the nucleotide sequence of IRES on therapy outcome, we compared HCV isolates from patients with sustained response and non-response to interferon/ribavirin combination therapy. In 56 analyzed HCV isolates, nucleotide changes appeared strictly in the stem-loop IIIb region, the stem part from 243 nt to 248 nt, and the polypyrimidine-II region. The natural sequence variability of IRES in isolates of genotype 3a was significantly higher than in isolates of genotype 1b (p LT 0.05). The average number of nucleotide changes in genotype 3a correlated with response to therapy (p LT 0.05).
T2  - Archives of Biological Sciences
T1  - Sequence Variability At the Internal Ribosome Entry Site of the Hcv Genome in Relation to Therapy Outcome
VL  - 61
IS  - 2
SP  - 205
EP  - 212
DO  - 10.2298/ABS0901205J
ER  - 

@article{
author = "Jovanović-Ćupić, Snežana P. and Simonovic-Babic, Jasmina and Blagojevic, Jelena and Božić, Milena and Ješić, Rada and Nozic, D. and Stamenković, Gorana",
year = "2009",
abstract = "Different types of interferon are widely used to treat hepatitis C virus (HCV) infection. Results obtained in vitro suggest that interferon inhibits internal ribosome entry site (IRES)-mediated translation of the HCV genome. To elucidate the possible effect of the nucleotide sequence of IRES on therapy outcome, we compared HCV isolates from patients with sustained response and non-response to interferon/ribavirin combination therapy. In 56 analyzed HCV isolates, nucleotide changes appeared strictly in the stem-loop IIIb region, the stem part from 243 nt to 248 nt, and the polypyrimidine-II region. The natural sequence variability of IRES in isolates of genotype 3a was significantly higher than in isolates of genotype 1b (p LT 0.05). The average number of nucleotide changes in genotype 3a correlated with response to therapy (p LT 0.05).",
journal = "Archives of Biological Sciences",
title = "Sequence Variability At the Internal Ribosome Entry Site of the Hcv Genome in Relation to Therapy Outcome",
volume = "61",
number = "2",
pages = "205-212",
doi = "10.2298/ABS0901205J"
}

Jovanović-Ćupić, S. P., Simonovic-Babic, J., Blagojevic, J., Božić, M., Ješić, R., Nozic, D.,& Stamenković, G.. (2009). Sequence Variability At the Internal Ribosome Entry Site of the Hcv Genome in Relation to Therapy Outcome. in Archives of Biological Sciences, 61(2), 205-212.
https://doi.org/10.2298/ABS0901205J

Jovanović-Ćupić SP, Simonovic-Babic J, Blagojevic J, Božić M, Ješić R, Nozic D, Stamenković G. Sequence Variability At the Internal Ribosome Entry Site of the Hcv Genome in Relation to Therapy Outcome. in Archives of Biological Sciences. 2009;61(2):205-212.
doi:10.2298/ABS0901205J .

Jovanović-Ćupić, Snežana P., Simonovic-Babic, Jasmina, Blagojevic, Jelena, Božić, Milena, Ješić, Rada, Nozic, D., Stamenković, Gorana, "Sequence Variability At the Internal Ribosome Entry Site of the Hcv Genome in Relation to Therapy Outcome" in Archives of Biological Sciences, 61, no. 2 (2009):205-212,
https://doi.org/10.2298/ABS0901205J . .

TY  - JOUR
AU  - Stamenković, Gorana
AU  - Božić, Milena
AU  - Jovanović-Ćupić, Snežana P.
AU  - Bojovic, Ksenija
AU  - Simonovic, Jasmina
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3417
T2  - Archives of Biological Sciences
T1  - Hepatitis C virus as cause of fulminant hepatitis-sequence analysis of the 5nontranslated region
VL  - 59
IS  - 3
SP  - 37P
EP  - 38P
DO  - 10.2298/ABS070337PS
ER  - 

@article{
author = "Stamenković, Gorana and Božić, Milena and Jovanović-Ćupić, Snežana P. and Bojovic, Ksenija and Simonovic, Jasmina",
year = "2007",
journal = "Archives of Biological Sciences",
title = "Hepatitis C virus as cause of fulminant hepatitis-sequence analysis of the 5nontranslated region",
volume = "59",
number = "3",
pages = "37P-38P",
doi = "10.2298/ABS070337PS"
}

Stamenković, G., Božić, M., Jovanović-Ćupić, S. P., Bojovic, K.,& Simonovic, J.. (2007). Hepatitis C virus as cause of fulminant hepatitis-sequence analysis of the 5nontranslated region. in Archives of Biological Sciences, 59(3), 37P-38P.
https://doi.org/10.2298/ABS070337PS

Stamenković G, Božić M, Jovanović-Ćupić SP, Bojovic K, Simonovic J. Hepatitis C virus as cause of fulminant hepatitis-sequence analysis of the 5nontranslated region. in Archives of Biological Sciences. 2007;59(3):37P-38P.
doi:10.2298/ABS070337PS .

Stamenković, Gorana, Božić, Milena, Jovanović-Ćupić, Snežana P., Bojovic, Ksenija, Simonovic, Jasmina, "Hepatitis C virus as cause of fulminant hepatitis-sequence analysis of the 5nontranslated region" in Archives of Biological Sciences, 59, no. 3 (2007):37P-38P,
https://doi.org/10.2298/ABS070337PS . .