Kanacki, Zdenko

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  • Kanacki, Zdenko (2)
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Author's Bibliography

Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue

Seke, Mariana; Petrović, Danijela; Đorđević, Aleksandar N.; Jović, Danica S.; Labudović-Borović, Milica; Kanacki, Zdenko; Jankovic, Milan

(2016)

TY  - JOUR
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Đorđević, Aleksandar N.
AU  - Jović, Danica S.
AU  - Labudović-Borović, Milica
AU  - Kanacki, Zdenko
AU  - Jankovic, Milan
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1306
AB  - Fullerenol (C-60(OH)(24)) is present in aqueous solutions in the form of polyanion nanoparticles with particles size distribution within the range from 15 to 42 nm. In this research it is assumed that these features could enable fullerenol nanoparticles (FNPs) to bind positively charged molecules like doxorubicin (DOX) and serve as drug carriers. Considering this, fullerenol/doxorubicin nanocomposite (FNP/DOX) is formed and characterized by ultra-performance liquid chromatography tandem mass spectrometry, dynamic light scattering, atomic force microscopy and transmission electron microscopy. Measurements have shown that DOX did not significantly affect particle size (23 nm). It is also assumed that FNP/DOX could reduce the acute cardiotoxic effects of DOX in vivo (Wistar rats treated i.p.). In this study, quantitative real time polymerase chain reaction results have shown that treatment with DOX alone caused significant increase in mRNA levels of catalase (p LT 0.05) enzyme indicating the presence of oxidative stress. This effect is significantly reduced by the treatment with FNP/DOX (p LT 0.05). Furthermore, mRNA levels of antiapoptotic enzyme (Bcl-2) are significantly increased (p LT 0.05) in all treated groups, particularly where FNP/DOX was applied, suggesting cell resistance to apoptosis. Moreover, ultrastructural analysis has shown the absence of myelin figures within the mitochondria in the heart tissue with FNP/DOX treatment, indicating reduction of oxidative stress. Hence, our results have implied that FNP/DOX is generally less harmful to the heart compared to DOX.
T2  - Nanotechnology
T1  - Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue
VL  - 27
IS  - 48
DO  - 10.1088/0957-4484/27/48/485101
ER  - 
@article{
author = "Seke, Mariana and Petrović, Danijela and Đorđević, Aleksandar N. and Jović, Danica S. and Labudović-Borović, Milica and Kanacki, Zdenko and Jankovic, Milan",
year = "2016",
abstract = "Fullerenol (C-60(OH)(24)) is present in aqueous solutions in the form of polyanion nanoparticles with particles size distribution within the range from 15 to 42 nm. In this research it is assumed that these features could enable fullerenol nanoparticles (FNPs) to bind positively charged molecules like doxorubicin (DOX) and serve as drug carriers. Considering this, fullerenol/doxorubicin nanocomposite (FNP/DOX) is formed and characterized by ultra-performance liquid chromatography tandem mass spectrometry, dynamic light scattering, atomic force microscopy and transmission electron microscopy. Measurements have shown that DOX did not significantly affect particle size (23 nm). It is also assumed that FNP/DOX could reduce the acute cardiotoxic effects of DOX in vivo (Wistar rats treated i.p.). In this study, quantitative real time polymerase chain reaction results have shown that treatment with DOX alone caused significant increase in mRNA levels of catalase (p LT 0.05) enzyme indicating the presence of oxidative stress. This effect is significantly reduced by the treatment with FNP/DOX (p LT 0.05). Furthermore, mRNA levels of antiapoptotic enzyme (Bcl-2) are significantly increased (p LT 0.05) in all treated groups, particularly where FNP/DOX was applied, suggesting cell resistance to apoptosis. Moreover, ultrastructural analysis has shown the absence of myelin figures within the mitochondria in the heart tissue with FNP/DOX treatment, indicating reduction of oxidative stress. Hence, our results have implied that FNP/DOX is generally less harmful to the heart compared to DOX.",
journal = "Nanotechnology",
title = "Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue",
volume = "27",
number = "48",
doi = "10.1088/0957-4484/27/48/485101"
}
Seke, M., Petrović, D., Đorđević, A. N., Jović, D. S., Labudović-Borović, M., Kanacki, Z.,& Jankovic, M.. (2016). Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue. in Nanotechnology, 27(48).
https://doi.org/10.1088/0957-4484/27/48/485101
Seke M, Petrović D, Đorđević AN, Jović DS, Labudović-Borović M, Kanacki Z, Jankovic M. Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue. in Nanotechnology. 2016;27(48).
doi:10.1088/0957-4484/27/48/485101 .
Seke, Mariana, Petrović, Danijela, Đorđević, Aleksandar N., Jović, Danica S., Labudović-Borović, Milica, Kanacki, Zdenko, Jankovic, Milan, "Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue" in Nanotechnology, 27, no. 48 (2016),
https://doi.org/10.1088/0957-4484/27/48/485101 . .
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Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study

Labudović-Borović, Milica; Icevic, Ivana; Kanacki, Zdenko; Zikic, Dragan; Seke, Mariana; Injac, Rade; Đorđević, Aleksandar N.

(2014)

TY  - JOUR
AU  - Labudović-Borović, Milica
AU  - Icevic, Ivana
AU  - Kanacki, Zdenko
AU  - Zikic, Dragan
AU  - Seke, Mariana
AU  - Injac, Rade
AU  - Đorđević, Aleksandar N.
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5924
AB  - Cardioprotective effects of fullerenol C-60(OH)(24) nanoparticles (FNP) were investigated in pigs after a single treatment with doxorubicin (DOX). Semithin and ultrathin sections of myocardial tissue routinely prepared for transmission electron microscopy were analyzed. Extensive intracellular damage was confirmed in cardiomyocytes of DOX-treated animals. By means of ultrastructural analysis, a certain degree of parenchymal degeneration was confirmed even in animals treated with FNP alone, including both the oral and the intraperitoneal application of the substance. The cardioprotective effects of FNP in animals previously treated with DOX were recognized to a certain extent, but were not fully confirmed at the ultrastructural level. Nevertheless, the myocardial morphology of DOX-treated animals improved after the admission of FNP. Irregular orientation of myofibrils, myofibrillar disruption, intracellular edema, and vacuolization were reduced, but not completely eliminated. Reduction of these cellular alterations was achieved if FNP was applied orally 6 h prior to DOX treatment in a dose of 18 mg/kg. However, numerous defects, including the inner mitochondrial membrane and the plasma membrane disruption of certain cells persisted. In FNP/DOX-treated animals, the presence of multinuclear cells with mitosis-like figures resembling metaphase or anaphase were observed, indicating that DOX and FNP could have a complex influence on the cell cycle of cardiomyocytes. Based on this experiment, further careful increase in dosage may be advised to enhance FNP-induced cardioprotection. These investigations should, however, always be combined with ultrastructural analysis. The FNP/DOX interaction is an excellent model for the investigation of cardiomyocyte cell death and cell cycle mechanisms.
T2  - Ultrastructural Pathology
T1  - Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study
VL  - 38
IS  - 2
SP  - 150
EP  - 163
DO  - 10.3109/01913123.2013.822045
ER  - 
@article{
author = "Labudović-Borović, Milica and Icevic, Ivana and Kanacki, Zdenko and Zikic, Dragan and Seke, Mariana and Injac, Rade and Đorđević, Aleksandar N.",
year = "2014",
abstract = "Cardioprotective effects of fullerenol C-60(OH)(24) nanoparticles (FNP) were investigated in pigs after a single treatment with doxorubicin (DOX). Semithin and ultrathin sections of myocardial tissue routinely prepared for transmission electron microscopy were analyzed. Extensive intracellular damage was confirmed in cardiomyocytes of DOX-treated animals. By means of ultrastructural analysis, a certain degree of parenchymal degeneration was confirmed even in animals treated with FNP alone, including both the oral and the intraperitoneal application of the substance. The cardioprotective effects of FNP in animals previously treated with DOX were recognized to a certain extent, but were not fully confirmed at the ultrastructural level. Nevertheless, the myocardial morphology of DOX-treated animals improved after the admission of FNP. Irregular orientation of myofibrils, myofibrillar disruption, intracellular edema, and vacuolization were reduced, but not completely eliminated. Reduction of these cellular alterations was achieved if FNP was applied orally 6 h prior to DOX treatment in a dose of 18 mg/kg. However, numerous defects, including the inner mitochondrial membrane and the plasma membrane disruption of certain cells persisted. In FNP/DOX-treated animals, the presence of multinuclear cells with mitosis-like figures resembling metaphase or anaphase were observed, indicating that DOX and FNP could have a complex influence on the cell cycle of cardiomyocytes. Based on this experiment, further careful increase in dosage may be advised to enhance FNP-induced cardioprotection. These investigations should, however, always be combined with ultrastructural analysis. The FNP/DOX interaction is an excellent model for the investigation of cardiomyocyte cell death and cell cycle mechanisms.",
journal = "Ultrastructural Pathology",
title = "Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study",
volume = "38",
number = "2",
pages = "150-163",
doi = "10.3109/01913123.2013.822045"
}
Labudović-Borović, M., Icevic, I., Kanacki, Z., Zikic, D., Seke, M., Injac, R.,& Đorđević, A. N.. (2014). Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study. in Ultrastructural Pathology, 38(2), 150-163.
https://doi.org/10.3109/01913123.2013.822045
Labudović-Borović M, Icevic I, Kanacki Z, Zikic D, Seke M, Injac R, Đorđević AN. Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study. in Ultrastructural Pathology. 2014;38(2):150-163.
doi:10.3109/01913123.2013.822045 .
Labudović-Borović, Milica, Icevic, Ivana, Kanacki, Zdenko, Zikic, Dragan, Seke, Mariana, Injac, Rade, Đorđević, Aleksandar N., "Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study" in Ultrastructural Pathology, 38, no. 2 (2014):150-163,
https://doi.org/10.3109/01913123.2013.822045 . .
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