Kovačević-Filipović, Milica M.

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  • Kovačević-Filipović, Milica M. (2)
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Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis

Milanović, Zorana; Beletić, Anđelo; Vekić, Jelena; Zeljković, Aleksandra; Andrić, Nenad; Ilić Božović, Anja; Spariosu, Kristina; Radaković, Milena; Ajtić, Jelena; Kovačević-Filipović, Milica M.

(2020)

TY  - JOUR
AU  - Milanović, Zorana
AU  - Beletić, Anđelo
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Andrić, Nenad
AU  - Ilić Božović, Anja
AU  - Spariosu, Kristina
AU  - Radaković, Milena
AU  - Ajtić, Jelena
AU  - Kovačević-Filipović, Milica M.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9011
AB  - Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.
T2  - Veterinary Parasitology
T1  - Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis
VL  - 282
SP  - 109140
DO  - 10.1016/j.vetpar.2020.109140
ER  - 
@article{
author = "Milanović, Zorana and Beletić, Anđelo and Vekić, Jelena and Zeljković, Aleksandra and Andrić, Nenad and Ilić Božović, Anja and Spariosu, Kristina and Radaković, Milena and Ajtić, Jelena and Kovačević-Filipović, Milica M.",
year = "2020",
abstract = "Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.",
journal = "Veterinary Parasitology",
title = "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis",
volume = "282",
pages = "109140",
doi = "10.1016/j.vetpar.2020.109140"
}
Milanović, Z., Beletić, A., Vekić, J., Zeljković, A., Andrić, N., Ilić Božović, A., Spariosu, K., Radaković, M., Ajtić, J.,& Kovačević-Filipović, M. M.. (2020). Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology, 282, 109140.
https://doi.org/10.1016/j.vetpar.2020.109140
Milanović Z, Beletić A, Vekić J, Zeljković A, Andrić N, Ilić Božović A, Spariosu K, Radaković M, Ajtić J, Kovačević-Filipović MM. Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology. 2020;282:109140.
doi:10.1016/j.vetpar.2020.109140 .
Milanović, Zorana, Beletić, Anđelo, Vekić, Jelena, Zeljković, Aleksandra, Andrić, Nenad, Ilić Božović, Anja, Spariosu, Kristina, Radaković, Milena, Ajtić, Jelena, Kovačević-Filipović, Milica M., "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis" in Veterinary Parasitology, 282 (2020):109140,
https://doi.org/10.1016/j.vetpar.2020.109140 . .
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Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas

Božić, Tatjana P.; Stevanović, Jelka Ž.; Kovačević-Filipović, Milica M.; Borozan, Sunčica Z.; Popović, Dragana Lj.; Todorović, Dragana

(2003)

TY  - JOUR
AU  - Božić, Tatjana P.
AU  - Stevanović, Jelka Ž.
AU  - Kovačević-Filipović, Milica M.
AU  - Borozan, Sunčica Z.
AU  - Popović, Dragana Lj.
AU  - Todorović, Dragana
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8767
AB  - The paper presents preliminary results of the study on environmental and health effects due to the use of depleted uranium (DU) ammunition during NATO bombing of Serbia and Montenegro in 1999. Samples of animal blood (sheep, cows) were randomly collected in the region of Bujanovac (Novo Selo, Borovac) in 2003. Biochemical and hematological parameters of peripheral blood (oxidase activity of ceruloplasmin, malondialdehide concentration of red blood cell, activity of erythrocytic superoxide dismutase, concentration of hemoglobin, red blood cells count, etc.) indicating possible effects of oxidative stress due to the impact of DU on the environment were analyzed. The results have been evaluated in respect to the effect of the environmental DU.
T2  - Central European Journal of Occupational and Environmetal Medicine
T1  - Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas
VL  - 9
IS  - 4
SP  - 267
EP  - 272
ER  - 
@article{
author = "Božić, Tatjana P. and Stevanović, Jelka Ž. and Kovačević-Filipović, Milica M. and Borozan, Sunčica Z. and Popović, Dragana Lj. and Todorović, Dragana",
year = "2003",
abstract = "The paper presents preliminary results of the study on environmental and health effects due to the use of depleted uranium (DU) ammunition during NATO bombing of Serbia and Montenegro in 1999. Samples of animal blood (sheep, cows) were randomly collected in the region of Bujanovac (Novo Selo, Borovac) in 2003. Biochemical and hematological parameters of peripheral blood (oxidase activity of ceruloplasmin, malondialdehide concentration of red blood cell, activity of erythrocytic superoxide dismutase, concentration of hemoglobin, red blood cells count, etc.) indicating possible effects of oxidative stress due to the impact of DU on the environment were analyzed. The results have been evaluated in respect to the effect of the environmental DU.",
journal = "Central European Journal of Occupational and Environmetal Medicine",
title = "Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas",
volume = "9",
number = "4",
pages = "267-272"
}
Božić, T. P., Stevanović, J. Ž., Kovačević-Filipović, M. M., Borozan, S. Z., Popović, D. Lj.,& Todorović, D.. (2003). Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas. in Central European Journal of Occupational and Environmetal Medicine, 9(4), 267-272.
Božić TP, Stevanović JŽ, Kovačević-Filipović MM, Borozan SZ, Popović DL, Todorović D. Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas. in Central European Journal of Occupational and Environmetal Medicine. 2003;9(4):267-272..
Božić, Tatjana P., Stevanović, Jelka Ž., Kovačević-Filipović, Milica M., Borozan, Sunčica Z., Popović, Dragana Lj., Todorović, Dragana, "Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas" in Central European Journal of Occupational and Environmetal Medicine, 9, no. 4 (2003):267-272.