TY  - JOUR
AU  - Barać, Milena
AU  - Petrović, Milan
AU  - Petrović, Nina
AU  - Nikolić-Jakoba, Nataša
AU  - Aleksić, Zoran
AU  - Todorović, Lidija
AU  - Petrović-Stanojević, Nataša
AU  - Anđelić-Jelić, Marina
AU  - Davidović, Aleksandar
AU  - Milašin, Jelena
AU  - Roganović, Jelena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11499
AB  - Type 2 diabetes mellitus (T2DM) is associated with functional deterioration of the salivary gland and dental pulp, related to oxidative stress. The aim was to integrate experimental and bioinformatic findings to analyze the cellular mechanism of melatonin (MEL) action in the human parotid gland and dental pulp in diabetes. Human parotid gland tissue was obtained from 16 non-diabetic and 16 diabetic participants, as well as human dental pulp from 15 non-diabetic and 15 diabetic participants. In human non-diabetic and diabetic parotid gland cells (hPGCs) as well as in dental pulp cells (hDPCs), cultured in hyper- and normoglycemic conditions, glial cell linederived neurotrophic factor (GDNF), MEL, inducible nitric oxide synthase (iNOS) protein expression, and superoxide dismutase (SOD) activity were measured by enzyme-linked immunosorbent assay (ELISA) and spectrophotometrically. Bioinformatic analysis was performed using ShinyGO (v.0.75) application. Diabetic participants had increased GDNF and decreased MEL in parotid (p < 0.01) and dental pulp (p < 0.05) tissues, associated with increased iNOS and SOD activity. Normoglycemic hDPCs and non-diabetic hPGCs treated with 0.1 mM MEL had increased GDNF (p < 0.05), while hyperglycemic hDPCs treated with 1 mM MEL showed a decrease in up-regulated GDNF (p < 0.05). Enrichment analyses showed interference with stress and ATF/CREB signaling. MEL induced the stress-protective mechanism in hyperglycemic hDPCs and diabetic hPGCs, suggesting MEL could be beneficial for diabetes-associated disturbances in oral tissues.
T2  - International Journal of Environmental Research and Public Health
T1  - Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings
VL  - 20
IS  - 18
SP  - 6727
DO  - 10.3390/ijerph20186727
ER  - 

@article{
author = "Barać, Milena and Petrović, Milan and Petrović, Nina and Nikolić-Jakoba, Nataša and Aleksić, Zoran and Todorović, Lidija and Petrović-Stanojević, Nataša and Anđelić-Jelić, Marina and Davidović, Aleksandar and Milašin, Jelena and Roganović, Jelena",
year = "2023",
abstract = "Type 2 diabetes mellitus (T2DM) is associated with functional deterioration of the salivary gland and dental pulp, related to oxidative stress. The aim was to integrate experimental and bioinformatic findings to analyze the cellular mechanism of melatonin (MEL) action in the human parotid gland and dental pulp in diabetes. Human parotid gland tissue was obtained from 16 non-diabetic and 16 diabetic participants, as well as human dental pulp from 15 non-diabetic and 15 diabetic participants. In human non-diabetic and diabetic parotid gland cells (hPGCs) as well as in dental pulp cells (hDPCs), cultured in hyper- and normoglycemic conditions, glial cell linederived neurotrophic factor (GDNF), MEL, inducible nitric oxide synthase (iNOS) protein expression, and superoxide dismutase (SOD) activity were measured by enzyme-linked immunosorbent assay (ELISA) and spectrophotometrically. Bioinformatic analysis was performed using ShinyGO (v.0.75) application. Diabetic participants had increased GDNF and decreased MEL in parotid (p < 0.01) and dental pulp (p < 0.05) tissues, associated with increased iNOS and SOD activity. Normoglycemic hDPCs and non-diabetic hPGCs treated with 0.1 mM MEL had increased GDNF (p < 0.05), while hyperglycemic hDPCs treated with 1 mM MEL showed a decrease in up-regulated GDNF (p < 0.05). Enrichment analyses showed interference with stress and ATF/CREB signaling. MEL induced the stress-protective mechanism in hyperglycemic hDPCs and diabetic hPGCs, suggesting MEL could be beneficial for diabetes-associated disturbances in oral tissues.",
journal = "International Journal of Environmental Research and Public Health",
title = "Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings",
volume = "20",
number = "18",
pages = "6727",
doi = "10.3390/ijerph20186727"
}

Barać, M., Petrović, M., Petrović, N., Nikolić-Jakoba, N., Aleksić, Z., Todorović, L., Petrović-Stanojević, N., Anđelić-Jelić, M., Davidović, A., Milašin, J.,& Roganović, J.. (2023). Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings. in International Journal of Environmental Research and Public Health, 20(18), 6727.
https://doi.org/10.3390/ijerph20186727

Barać M, Petrović M, Petrović N, Nikolić-Jakoba N, Aleksić Z, Todorović L, Petrović-Stanojević N, Anđelić-Jelić M, Davidović A, Milašin J, Roganović J. Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings. in International Journal of Environmental Research and Public Health. 2023;20(18):6727.
doi:10.3390/ijerph20186727 .

Barać, Milena, Petrović, Milan, Petrović, Nina, Nikolić-Jakoba, Nataša, Aleksić, Zoran, Todorović, Lidija, Petrović-Stanojević, Nataša, Anđelić-Jelić, Marina, Davidović, Aleksandar, Milašin, Jelena, Roganović, Jelena, "Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings" in International Journal of Environmental Research and Public Health, 20, no. 18 (2023):6727,
https://doi.org/10.3390/ijerph20186727 . .

TY  - JOUR
AU  - Micić, Milutin
AU  - Antonijević, Đorđe
AU  - Milutinović-Smiljanić, Sanja
AU  - Trišić, Dijana
AU  - Čolović, Božana M.
AU  - Kosanović, Dejana
AU  - Prokić, Bogomir Bolka
AU  - Vasić, Jugoslav
AU  - Živković, Slavoljub
AU  - Milašin, Jelena
AU  - Danilović, Vesna
AU  - Đurić, Marija P.
AU  - Jokanović, Vukoman R.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10363
AB  - Article Corrigendum to: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit’s ulna reconstruction was published on June 28, 2022 in the journal Biomedical Engineering / Biomedizinische Technik
T2  - Biomedical Engineering / Biomedizinische Technik
T1  - Corrigendum: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction (Biomedical Engineering / Biomedizinische Technik 65:4 (491-505) DOI: 10.1515/bmt-2019-0218)
DO  - 10.1515/bmt-2022-0188
ER  - 

@article{
author = "Micić, Milutin and Antonijević, Đorđe and Milutinović-Smiljanić, Sanja and Trišić, Dijana and Čolović, Božana M. and Kosanović, Dejana and Prokić, Bogomir Bolka and Vasić, Jugoslav and Živković, Slavoljub and Milašin, Jelena and Danilović, Vesna and Đurić, Marija P. and Jokanović, Vukoman R.",
year = "2022",
abstract = "Article Corrigendum to: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit’s ulna reconstruction was published on June 28, 2022 in the journal Biomedical Engineering / Biomedizinische Technik",
journal = "Biomedical Engineering / Biomedizinische Technik",
title = "Corrigendum: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction (Biomedical Engineering / Biomedizinische Technik 65:4 (491-505) DOI: 10.1515/bmt-2019-0218)",
doi = "10.1515/bmt-2022-0188"
}

Micić, M., Antonijević, Đ., Milutinović-Smiljanić, S., Trišić, D., Čolović, B. M., Kosanović, D., Prokić, B. B., Vasić, J., Živković, S., Milašin, J., Danilović, V., Đurić, M. P.,& Jokanović, V. R.. (2022). Corrigendum: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction (Biomedical Engineering / Biomedizinische Technik 65:4 (491-505) DOI: 10.1515/bmt-2019-0218). in Biomedical Engineering / Biomedizinische Technik.
https://doi.org/10.1515/bmt-2022-0188

Micić M, Antonijević Đ, Milutinović-Smiljanić S, Trišić D, Čolović BM, Kosanović D, Prokić BB, Vasić J, Živković S, Milašin J, Danilović V, Đurić MP, Jokanović VR. Corrigendum: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction (Biomedical Engineering / Biomedizinische Technik 65:4 (491-505) DOI: 10.1515/bmt-2019-0218). in Biomedical Engineering / Biomedizinische Technik. 2022;.
doi:10.1515/bmt-2022-0188 .

Micić, Milutin, Antonijević, Đorđe, Milutinović-Smiljanić, Sanja, Trišić, Dijana, Čolović, Božana M., Kosanović, Dejana, Prokić, Bogomir Bolka, Vasić, Jugoslav, Živković, Slavoljub, Milašin, Jelena, Danilović, Vesna, Đurić, Marija P., Jokanović, Vukoman R., "Corrigendum: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction (Biomedical Engineering / Biomedizinische Technik 65:4 (491-505) DOI: 10.1515/bmt-2019-0218)" in Biomedical Engineering / Biomedizinische Technik (2022),
https://doi.org/10.1515/bmt-2022-0188 . .

TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Despotović, Ana
AU  - Biočanin, Vladimir
AU  - Milošević, Miloš
AU  - Trišić, Dijana
AU  - Lazović, Vladimir M.
AU  - Zogović, Nevena
AU  - Milašin, Jelena
AU  - Ilić, Dragan V.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9880
AB  - The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p < 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p < 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p < 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p < 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p < 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements’ surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.
T2  - Ceramics International
T1  - Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic
VL  - 47
IS  - 20
SP  - 28913
EP  - 28923
DO  - 10.1016/j.ceramint.2021.07.052
ER  - 

@article{
author = "Antonijević, Đorđe and Despotović, Ana and Biočanin, Vladimir and Milošević, Miloš and Trišić, Dijana and Lazović, Vladimir M. and Zogović, Nevena and Milašin, Jelena and Ilić, Dragan V.",
year = "2021",
abstract = "The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p < 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p < 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p < 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p < 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p < 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements’ surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.",
journal = "Ceramics International",
title = "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic",
volume = "47",
number = "20",
pages = "28913-28923",
doi = "10.1016/j.ceramint.2021.07.052"
}

Antonijević, Đ., Despotović, A., Biočanin, V., Milošević, M., Trišić, D., Lazović, V. M., Zogović, N., Milašin, J.,& Ilić, D. V.. (2021). Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International, 47(20), 28913-28923.
https://doi.org/10.1016/j.ceramint.2021.07.052

Antonijević Đ, Despotović A, Biočanin V, Milošević M, Trišić D, Lazović VM, Zogović N, Milašin J, Ilić DV. Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International. 2021;47(20):28913-28923.
doi:10.1016/j.ceramint.2021.07.052 .

Antonijević, Đorđe, Despotović, Ana, Biočanin, Vladimir, Milošević, Miloš, Trišić, Dijana, Lazović, Vladimir M., Zogović, Nevena, Milašin, Jelena, Ilić, Dragan V., "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic" in Ceramics International, 47, no. 20 (2021):28913-28923,
https://doi.org/10.1016/j.ceramint.2021.07.052 . .

TY  - JOUR
AU  - Micić, Milutin
AU  - Antonijević, Đorđe
AU  - Milutinović-Smiljanić, Sanja
AU  - Trišić, Dijana
AU  - Čolović, Božana M.
AU  - Kosanović, Dejana
AU  - Prokić, Bogomir Bolka
AU  - Vasić, Jugoslav
AU  - Živković, Slavoljub
AU  - Milašin, Jelena
AU  - Danilović, Vesna
AU  - Đurić, Marija P.
AU  - Jokanović, Vukoman R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8993
AB  - The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators-bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.
T2  - Biomedizinische Technik
T1  - Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction
VL  - 65
IS  - 4
SP  - 491
EP  - 505
DO  - 10.1515/bmt-2019-0218
ER  - 

@article{
author = "Micić, Milutin and Antonijević, Đorđe and Milutinović-Smiljanić, Sanja and Trišić, Dijana and Čolović, Božana M. and Kosanović, Dejana and Prokić, Bogomir Bolka and Vasić, Jugoslav and Živković, Slavoljub and Milašin, Jelena and Danilović, Vesna and Đurić, Marija P. and Jokanović, Vukoman R.",
year = "2020",
abstract = "The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators-bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.",
journal = "Biomedizinische Technik",
title = "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction",
volume = "65",
number = "4",
pages = "491-505",
doi = "10.1515/bmt-2019-0218"
}

Micić, M., Antonijević, Đ., Milutinović-Smiljanić, S., Trišić, D., Čolović, B. M., Kosanović, D., Prokić, B. B., Vasić, J., Živković, S., Milašin, J., Danilović, V., Đurić, M. P.,& Jokanović, V. R.. (2020). Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedizinische Technik, 65(4), 491-505.
https://doi.org/10.1515/bmt-2019-0218

Micić M, Antonijević Đ, Milutinović-Smiljanić S, Trišić D, Čolović BM, Kosanović D, Prokić BB, Vasić J, Živković S, Milašin J, Danilović V, Đurić MP, Jokanović VR. Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedizinische Technik. 2020;65(4):491-505.
doi:10.1515/bmt-2019-0218 .

Micić, Milutin, Antonijević, Đorđe, Milutinović-Smiljanić, Sanja, Trišić, Dijana, Čolović, Božana M., Kosanović, Dejana, Prokić, Bogomir Bolka, Vasić, Jugoslav, Živković, Slavoljub, Milašin, Jelena, Danilović, Vesna, Đurić, Marija P., Jokanović, Vukoman R., "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction" in Biomedizinische Technik, 65, no. 4 (2020):491-505,
https://doi.org/10.1515/bmt-2019-0218 . .

TY  - JOUR
AU  - Nikolić, Nađa
AU  - Čarkić, Jelena
AU  - Ilić Dimitrijević, Ivana
AU  - Eljabo, Najib
AU  - Radunović, Milena
AU  - Aničić, Boban
AU  - Tanić, Nasta
AU  - Falk, Markus
AU  - Milašin, Jelena
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1862
AB  - Objective. To investigate the prevalence of p16(INK4) (a), p14(ARF), tumor protein p53 (TP53), and human telomerase reverse transcriptase (hTERT) promoter hypermethylation in mucoepidermoid carcinomas (MECs) and search for a possible association between methylation status and clinicopathological parameters. Study design. DNA extracted from 35 formalin-fixed and paraffin-embedded MEC samples and 10 normal salivary gland (NSG) tissue samples was analyzed for the presence of promoter hypermethylation using methylation-specific polymerase chain reaction testing. Results. The percentages of gene hypermethylation in MECs versus NSGs were the following: p14: 100% versus 20% (P LT .001); p16: 60% versus 20% (P = .035); hTERT: 54.3% versus 20% (P = .078); and TP53: 31.4% versus 30% (P = .981). Multiple sites were found to be methylated in 86% of MECs compared with 10% in NSGs (P LT .001). TP53 and hTERT were more often methylated in lower clinical stages (P = .033 and P = .005, respectively). Conclusions. Hypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters.
T2  - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
T1  - P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population
VL  - 125
IS  - 1
SP  - 52
EP  - 58
DO  - 10.1016/j.oooo.2017.09.013
ER  - 

@article{
author = "Nikolić, Nađa and Čarkić, Jelena and Ilić Dimitrijević, Ivana and Eljabo, Najib and Radunović, Milena and Aničić, Boban and Tanić, Nasta and Falk, Markus and Milašin, Jelena",
year = "2018",
abstract = "Objective. To investigate the prevalence of p16(INK4) (a), p14(ARF), tumor protein p53 (TP53), and human telomerase reverse transcriptase (hTERT) promoter hypermethylation in mucoepidermoid carcinomas (MECs) and search for a possible association between methylation status and clinicopathological parameters. Study design. DNA extracted from 35 formalin-fixed and paraffin-embedded MEC samples and 10 normal salivary gland (NSG) tissue samples was analyzed for the presence of promoter hypermethylation using methylation-specific polymerase chain reaction testing. Results. The percentages of gene hypermethylation in MECs versus NSGs were the following: p14: 100% versus 20% (P LT .001); p16: 60% versus 20% (P = .035); hTERT: 54.3% versus 20% (P = .078); and TP53: 31.4% versus 30% (P = .981). Multiple sites were found to be methylated in 86% of MECs compared with 10% in NSGs (P LT .001). TP53 and hTERT were more often methylated in lower clinical stages (P = .033 and P = .005, respectively). Conclusions. Hypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters.",
journal = "Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology",
title = "P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population",
volume = "125",
number = "1",
pages = "52-58",
doi = "10.1016/j.oooo.2017.09.013"
}

Nikolić, N., Čarkić, J., Ilić Dimitrijević, I., Eljabo, N., Radunović, M., Aničić, B., Tanić, N., Falk, M.,& Milašin, J.. (2018). P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population. in Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 125(1), 52-58.
https://doi.org/10.1016/j.oooo.2017.09.013

Nikolić N, Čarkić J, Ilić Dimitrijević I, Eljabo N, Radunović M, Aničić B, Tanić N, Falk M, Milašin J. P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population. in Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 2018;125(1):52-58.
doi:10.1016/j.oooo.2017.09.013 .

Nikolić, Nađa, Čarkić, Jelena, Ilić Dimitrijević, Ivana, Eljabo, Najib, Radunović, Milena, Aničić, Boban, Tanić, Nasta, Falk, Markus, Milašin, Jelena, "P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population" in Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 125, no. 1 (2018):52-58,
https://doi.org/10.1016/j.oooo.2017.09.013 . .

TY  - JOUR
AU  - Eljabo, Najib
AU  - Nikolić, Nađa
AU  - Čarkić, Jelena
AU  - Jelovac, Drago B.
AU  - Lazarević, Miloš M.
AU  - Tanić, Nasta
AU  - Milašin, Jelena
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0901502718300389
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7924
AB  - Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers. c-Myc and HER2 genes were quantified by means of real-time PCR (qPCR), and p14 and p16 methylation status was determined by methylation-specific PCR (MSP PCR). All tissues examined exhibited molecular alterations in various proportions. Tumour tissues, as expected, showed the highest prevalence of alterations, while oral mucosa showed the lowest. Multiple alterations (co-alterations) in tumours and tumour margins were significantly more frequent than in unaffected oral mucosa (P < 0.001 and P = 0.027, respectively). HER2 amplification in margin tissue (P < 0.001) and swabs (P = 0.013), as well as the existence of three co-alterations in margins (P = 0.001) and macroscopically unaffected oral mucosa (P < 0.001) were correlated with shorter disease-specific survival.
T2  - International Journal of Oral and Maxillofacial Surgery
T1  - Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer
VL  - 47
IS  - 8
SP  - 976
EP  - 982
DO  - 10.1016/j.ijom.2018.01.020
ER  - 

@article{
author = "Eljabo, Najib and Nikolić, Nađa and Čarkić, Jelena and Jelovac, Drago B. and Lazarević, Miloš M. and Tanić, Nasta and Milašin, Jelena",
year = "2018",
abstract = "Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers. c-Myc and HER2 genes were quantified by means of real-time PCR (qPCR), and p14 and p16 methylation status was determined by methylation-specific PCR (MSP PCR). All tissues examined exhibited molecular alterations in various proportions. Tumour tissues, as expected, showed the highest prevalence of alterations, while oral mucosa showed the lowest. Multiple alterations (co-alterations) in tumours and tumour margins were significantly more frequent than in unaffected oral mucosa (P < 0.001 and P = 0.027, respectively). HER2 amplification in margin tissue (P < 0.001) and swabs (P = 0.013), as well as the existence of three co-alterations in margins (P = 0.001) and macroscopically unaffected oral mucosa (P < 0.001) were correlated with shorter disease-specific survival.",
journal = "International Journal of Oral and Maxillofacial Surgery",
title = "Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer",
volume = "47",
number = "8",
pages = "976-982",
doi = "10.1016/j.ijom.2018.01.020"
}

Eljabo, N., Nikolić, N., Čarkić, J., Jelovac, D. B., Lazarević, M. M., Tanić, N.,& Milašin, J.. (2018). Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer. in International Journal of Oral and Maxillofacial Surgery, 47(8), 976-982.
https://doi.org/10.1016/j.ijom.2018.01.020

Eljabo N, Nikolić N, Čarkić J, Jelovac DB, Lazarević MM, Tanić N, Milašin J. Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer. in International Journal of Oral and Maxillofacial Surgery. 2018;47(8):976-982.
doi:10.1016/j.ijom.2018.01.020 .

Eljabo, Najib, Nikolić, Nađa, Čarkić, Jelena, Jelovac, Drago B., Lazarević, Miloš M., Tanić, Nasta, Milašin, Jelena, "Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer" in International Journal of Oral and Maxillofacial Surgery, 47, no. 8 (2018):976-982,
https://doi.org/10.1016/j.ijom.2018.01.020 . .

TY  - JOUR
AU  - Nikolić, Nađa
AU  - Aničić, Boban
AU  - Čarkić, Jelena
AU  - Simonovic, Jelena
AU  - Toljic, Bosko
AU  - Tanić, Nasta
AU  - Tepavcevic, Zvezdana
AU  - Vukadinovic, Miroslav
AU  - Konstantinovic, Vitomir S.
AU  - Milašin, Jelena
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/778
AB  - Objectives: to investigate p16(INK4a) and p14(ARF) tumor suppressor gene methylation status, determine telomere length and assess the importance of these epigenetic and genetic parameters in the development of pleomorphic adenoma and carcinoma ex pleomorphic adenoma of the parotid salivary glands. Materials and Methods: Genomic DNA from paraffin-embedded samples of 50 pleomorphic adenomas and 10 carcinomas ex pleomorphic adenoma was subjected to methylation specific polymerase chain reaction for hypermethylation analyses and real time polymerase chain reaction for the relative telomere length calculations. Results: Promoter hypermethylation of the two genes was a very frequent event in both neoplasms between 60% and 90% of samples were hypermethylated - but without significant difference between the groups. The mean relative telomere length in the pleomorphic adenoma group was significantly increased in comparison to the control group (P = 0.00), and significantly decreased in comparison to the carcinoma group (P = 0.05). Telomeres were also longer in myxoid and cellular histological subtypes of adenomas than in the classic type (P = 0.044 and P = 0.018, respectively). Longer telomeres were more frequent in tumors with hypermethylated p14(ARF) alleles (P = 0.013). Conclusion: Promoter hypermethylations seems to be an important mechanism of p16(INK4a) and Pl4(ARF) inactivation in parotid gland tumors. Telomeric lengthening appears to be involved in the pathogenesis of both benign and malignant tumors of the parotid glands. (C) 2015 Elsevier Ltd. All rights reserved.
T2  - Archives of Oral Biology
T1  - High frequency of p16 and p14 promoter hypermethylation and marked telomere instability in salivary gland tumors
VL  - 60
IS  - 11
SP  - 1662
EP  - 1666
DO  - 10.1016/j.archoralbio.2015.08.011
ER  - 

@article{
author = "Nikolić, Nađa and Aničić, Boban and Čarkić, Jelena and Simonovic, Jelena and Toljic, Bosko and Tanić, Nasta and Tepavcevic, Zvezdana and Vukadinovic, Miroslav and Konstantinovic, Vitomir S. and Milašin, Jelena",
year = "2015",
abstract = "Objectives: to investigate p16(INK4a) and p14(ARF) tumor suppressor gene methylation status, determine telomere length and assess the importance of these epigenetic and genetic parameters in the development of pleomorphic adenoma and carcinoma ex pleomorphic adenoma of the parotid salivary glands. Materials and Methods: Genomic DNA from paraffin-embedded samples of 50 pleomorphic adenomas and 10 carcinomas ex pleomorphic adenoma was subjected to methylation specific polymerase chain reaction for hypermethylation analyses and real time polymerase chain reaction for the relative telomere length calculations. Results: Promoter hypermethylation of the two genes was a very frequent event in both neoplasms between 60% and 90% of samples were hypermethylated - but without significant difference between the groups. The mean relative telomere length in the pleomorphic adenoma group was significantly increased in comparison to the control group (P = 0.00), and significantly decreased in comparison to the carcinoma group (P = 0.05). Telomeres were also longer in myxoid and cellular histological subtypes of adenomas than in the classic type (P = 0.044 and P = 0.018, respectively). Longer telomeres were more frequent in tumors with hypermethylated p14(ARF) alleles (P = 0.013). Conclusion: Promoter hypermethylations seems to be an important mechanism of p16(INK4a) and Pl4(ARF) inactivation in parotid gland tumors. Telomeric lengthening appears to be involved in the pathogenesis of both benign and malignant tumors of the parotid glands. (C) 2015 Elsevier Ltd. All rights reserved.",
journal = "Archives of Oral Biology",
title = "High frequency of p16 and p14 promoter hypermethylation and marked telomere instability in salivary gland tumors",
volume = "60",
number = "11",
pages = "1662-1666",
doi = "10.1016/j.archoralbio.2015.08.011"
}

Nikolić, N., Aničić, B., Čarkić, J., Simonovic, J., Toljic, B., Tanić, N., Tepavcevic, Z., Vukadinovic, M., Konstantinovic, V. S.,& Milašin, J.. (2015). High frequency of p16 and p14 promoter hypermethylation and marked telomere instability in salivary gland tumors. in Archives of Oral Biology, 60(11), 1662-1666.
https://doi.org/10.1016/j.archoralbio.2015.08.011

Nikolić N, Aničić B, Čarkić J, Simonovic J, Toljic B, Tanić N, Tepavcevic Z, Vukadinovic M, Konstantinovic VS, Milašin J. High frequency of p16 and p14 promoter hypermethylation and marked telomere instability in salivary gland tumors. in Archives of Oral Biology. 2015;60(11):1662-1666.
doi:10.1016/j.archoralbio.2015.08.011 .

Nikolić, Nađa, Aničić, Boban, Čarkić, Jelena, Simonovic, Jelena, Toljic, Bosko, Tanić, Nasta, Tepavcevic, Zvezdana, Vukadinovic, Miroslav, Konstantinovic, Vitomir S., Milašin, Jelena, "High frequency of p16 and p14 promoter hypermethylation and marked telomere instability in salivary gland tumors" in Archives of Oral Biology, 60, no. 11 (2015):1662-1666,
https://doi.org/10.1016/j.archoralbio.2015.08.011 . .

TY  - JOUR
AU  - Nikolic, Nada
AU  - Aničić, Boban
AU  - Tepavcevic, Zvezdana
AU  - Jezdic, Zoran
AU  - Čarkić, Jelena
AU  - Toljic, Bosko
AU  - Dedović-Tanić, Nasta
AU  - Konstantinovic, Vitomir
AU  - Vukadinovic, Miroslav
AU  - Milašin, Jelena
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5746
AB  - Background: Genetic studies of salivary gland neoplasms were mainly focused on chromosomal changes, and some specific patterns of chromosome translocations have been described. However, molecular alterations, in particular the role of HER-2/H-ras/c-myc signalling cascade in pleomorphic adenoma pathogenesis (PA), are less well characterized. In addition, data on single nucleotide polymorphisms (SNPs) as potential susceptibility factors for PA development are also quite scarce. Methods: Mutational analyses were performed by means of real-time PCR (HER-2 and c-myc amplification analysis), PCR-SSCP and sequencing (H-ras point mutation detection). Polymorphisms analysis was performed by PCR-RFLP (survivin and MMP-9 genes). Results: Amplification of HER-2 and c-myc has been found in 13% and 9% of PA cases respectively. Point mutations in H-ras codons 12/13 have been detected in 17% of PAs. No correlation could be established between these alterations and clinical characteristics of PAs, whereas they might play a role in a subset of malignant salivary gland tumours. As for survivin -31 G/C polymorphism, C allele carriers had a 4-fold decrease of the risk of developing PA (p=0.05). Carriers of the variant allele T of the -1562C/T SNP in MMP-9 gene had a 4-fold increase of the risk of developing PA (p LT 0.001). Conclusions: A longer follow-up of PA patients harbouring mutations could uncover a prognostic role of HER-2 and c-myc amplification as predictors of adenoma transformation into carcinoma. Both survivin and MMP-9 promoter polymorphisms represent susceptibility factors for the development of PAs in the Serbian population.
T2  - Journal of Medical Biochemistry
T1  - Somatic Mutation and Polymorphism Analysis in Pleomorphic Adenomas of the Salivary Glands
VL  - 32
IS  - 4
SP  - 354
EP  - 360
DO  - 10.2478/jomb-2013-0048
ER  - 

@article{
author = "Nikolic, Nada and Aničić, Boban and Tepavcevic, Zvezdana and Jezdic, Zoran and Čarkić, Jelena and Toljic, Bosko and Dedović-Tanić, Nasta and Konstantinovic, Vitomir and Vukadinovic, Miroslav and Milašin, Jelena",
year = "2013",
abstract = "Background: Genetic studies of salivary gland neoplasms were mainly focused on chromosomal changes, and some specific patterns of chromosome translocations have been described. However, molecular alterations, in particular the role of HER-2/H-ras/c-myc signalling cascade in pleomorphic adenoma pathogenesis (PA), are less well characterized. In addition, data on single nucleotide polymorphisms (SNPs) as potential susceptibility factors for PA development are also quite scarce. Methods: Mutational analyses were performed by means of real-time PCR (HER-2 and c-myc amplification analysis), PCR-SSCP and sequencing (H-ras point mutation detection). Polymorphisms analysis was performed by PCR-RFLP (survivin and MMP-9 genes). Results: Amplification of HER-2 and c-myc has been found in 13% and 9% of PA cases respectively. Point mutations in H-ras codons 12/13 have been detected in 17% of PAs. No correlation could be established between these alterations and clinical characteristics of PAs, whereas they might play a role in a subset of malignant salivary gland tumours. As for survivin -31 G/C polymorphism, C allele carriers had a 4-fold decrease of the risk of developing PA (p=0.05). Carriers of the variant allele T of the -1562C/T SNP in MMP-9 gene had a 4-fold increase of the risk of developing PA (p LT 0.001). Conclusions: A longer follow-up of PA patients harbouring mutations could uncover a prognostic role of HER-2 and c-myc amplification as predictors of adenoma transformation into carcinoma. Both survivin and MMP-9 promoter polymorphisms represent susceptibility factors for the development of PAs in the Serbian population.",
journal = "Journal of Medical Biochemistry",
title = "Somatic Mutation and Polymorphism Analysis in Pleomorphic Adenomas of the Salivary Glands",
volume = "32",
number = "4",
pages = "354-360",
doi = "10.2478/jomb-2013-0048"
}

Nikolic, N., Aničić, B., Tepavcevic, Z., Jezdic, Z., Čarkić, J., Toljic, B., Dedović-Tanić, N., Konstantinovic, V., Vukadinovic, M.,& Milašin, J.. (2013). Somatic Mutation and Polymorphism Analysis in Pleomorphic Adenomas of the Salivary Glands. in Journal of Medical Biochemistry, 32(4), 354-360.
https://doi.org/10.2478/jomb-2013-0048

Nikolic N, Aničić B, Tepavcevic Z, Jezdic Z, Čarkić J, Toljic B, Dedović-Tanić N, Konstantinovic V, Vukadinovic M, Milašin J. Somatic Mutation and Polymorphism Analysis in Pleomorphic Adenomas of the Salivary Glands. in Journal of Medical Biochemistry. 2013;32(4):354-360.
doi:10.2478/jomb-2013-0048 .

Nikolic, Nada, Aničić, Boban, Tepavcevic, Zvezdana, Jezdic, Zoran, Čarkić, Jelena, Toljic, Bosko, Dedović-Tanić, Nasta, Konstantinovic, Vitomir, Vukadinovic, Miroslav, Milašin, Jelena, "Somatic Mutation and Polymorphism Analysis in Pleomorphic Adenomas of the Salivary Glands" in Journal of Medical Biochemistry, 32, no. 4 (2013):354-360,
https://doi.org/10.2478/jomb-2013-0048 . .

TY  - JOUR
AU  - Tanić, Nasta
AU  - Milašin, Jelena
AU  - Dramićanin, Tatjana
AU  - Bošković, Maja
AU  - Vukadinovic, Miroslav
AU  - Milošević, Verica
AU  - Tanić, Nikola
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5747
AB  - Background: Head and neck squamous cell carcinoma, including oral cancer, is the sixth most common cancer worldwide. Despite advances in surgery and treatment, the 5-year survival rate has not improved significantly. There fore, reliable molecular markers for oral cancer progression are badly needed. Methods: We conducted a copy number analysis to estimate amplification status of c-myc, cycD1 and EGFR oncogenes, mutational PCR-SSCP analysis to determine activation of H-ras oncogene and inactivation of TP53 tumour suppressor gene and methylation specific PCR analysis to evaluate hypermethylation of p16 and MGMT genes. Results: c-myc oncogene was amplified in 56.7%, cycD1 in 20% and EGFR in 16.7% of Oral Squamous Cell Carcinoma (OSCC) cases while H-ras was activated in 33.3% of samples. Amplification of c-myc was significantly associated with the tumour grade 2. Interestingly, EGFR and H-ras alterations were mutually exclusive. p16 and MGMT were inactivated by hypermethylation in 30% and 13.3% of cases. Co-alteration of cycD1 and p16 were not observed in any of the analyzed samples. TP53 was inactivated in 56.7% of samples and was significantly associated with progression of OSCC, grade 2 and stage 2. Moreover, TP53 and c-myc oncogene were simultaneously altered in grade 2 OSCC. Conclusions: The most promising marker of OSCC progression remains the TP53 tumour suppressor, which is the most frequently mutated gene in oral cancers. Since there is synergism between TP53 and c-myc, it seems that co-alteration of these two genes could be also a good marker of OSCC progression from grade1 to grade 2 tumours.
T2  - Journal of Medical Biochemistry
T1  - TP53 AND C-MYC CO-ALTERATIONS - A HALLMARK OF ORAL CANCER PROGRESSION
VL  - 32
IS  - 4
SP  - 380
EP  - 388
DO  - 10.2478/jomb-2014-0009
ER  - 

@article{
author = "Tanić, Nasta and Milašin, Jelena and Dramićanin, Tatjana and Bošković, Maja and Vukadinovic, Miroslav and Milošević, Verica and Tanić, Nikola",
year = "2013",
abstract = "Background: Head and neck squamous cell carcinoma, including oral cancer, is the sixth most common cancer worldwide. Despite advances in surgery and treatment, the 5-year survival rate has not improved significantly. There fore, reliable molecular markers for oral cancer progression are badly needed. Methods: We conducted a copy number analysis to estimate amplification status of c-myc, cycD1 and EGFR oncogenes, mutational PCR-SSCP analysis to determine activation of H-ras oncogene and inactivation of TP53 tumour suppressor gene and methylation specific PCR analysis to evaluate hypermethylation of p16 and MGMT genes. Results: c-myc oncogene was amplified in 56.7%, cycD1 in 20% and EGFR in 16.7% of Oral Squamous Cell Carcinoma (OSCC) cases while H-ras was activated in 33.3% of samples. Amplification of c-myc was significantly associated with the tumour grade 2. Interestingly, EGFR and H-ras alterations were mutually exclusive. p16 and MGMT were inactivated by hypermethylation in 30% and 13.3% of cases. Co-alteration of cycD1 and p16 were not observed in any of the analyzed samples. TP53 was inactivated in 56.7% of samples and was significantly associated with progression of OSCC, grade 2 and stage 2. Moreover, TP53 and c-myc oncogene were simultaneously altered in grade 2 OSCC. Conclusions: The most promising marker of OSCC progression remains the TP53 tumour suppressor, which is the most frequently mutated gene in oral cancers. Since there is synergism between TP53 and c-myc, it seems that co-alteration of these two genes could be also a good marker of OSCC progression from grade1 to grade 2 tumours.",
journal = "Journal of Medical Biochemistry",
title = "TP53 AND C-MYC CO-ALTERATIONS - A HALLMARK OF ORAL CANCER PROGRESSION",
volume = "32",
number = "4",
pages = "380-388",
doi = "10.2478/jomb-2014-0009"
}

Tanić, N., Milašin, J., Dramićanin, T., Bošković, M., Vukadinovic, M., Milošević, V.,& Tanić, N.. (2013). TP53 AND C-MYC CO-ALTERATIONS - A HALLMARK OF ORAL CANCER PROGRESSION. in Journal of Medical Biochemistry, 32(4), 380-388.
https://doi.org/10.2478/jomb-2014-0009

Tanić N, Milašin J, Dramićanin T, Bošković M, Vukadinovic M, Milošević V, Tanić N. TP53 AND C-MYC CO-ALTERATIONS - A HALLMARK OF ORAL CANCER PROGRESSION. in Journal of Medical Biochemistry. 2013;32(4):380-388.
doi:10.2478/jomb-2014-0009 .

Tanić, Nasta, Milašin, Jelena, Dramićanin, Tatjana, Bošković, Maja, Vukadinovic, Miroslav, Milošević, Verica, Tanić, Nikola, "TP53 AND C-MYC CO-ALTERATIONS - A HALLMARK OF ORAL CANCER PROGRESSION" in Journal of Medical Biochemistry, 32, no. 4 (2013):380-388,
https://doi.org/10.2478/jomb-2014-0009 . .

TY  - JOUR
AU  - Tanić, Nikola
AU  - Tanić, Nikola
AU  - Milašin, Jelena
AU  - Vukadinovic, Miroslav
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3692
AB  - Leukoplakias, clinically identifiable premalignant lesions, often precede oral squamous cell carcinoma (OSCC). Identification of leukoplakias that have the potential for transformation to malignancy is a key clinical problem. The aim of this study was to assess genomic instability, and to detect tumor-specific genomic alterations, in leukoplakias. Genomic instability was analyzed by comparing the DNA fingerprints of 32 leukoplakias with those of paired normal tissue. In addition, the mutational status of the p53 gene was analyzed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex DNA (PCR-HET), and the mutations were subsequently confirmed by DNA sequencing. Moderate-to-significant genomic instability was detected in all leukoplakias analysed. Nine unique amplicons, present in leukoplakias but not in normal tissue, were retrieved and successfully characterized. The p53 gene was mutated in 40.6% of patients. Four patients with moderate instability and mutated p53 developed OSCC. The data obtained in this study support and concretize the thesis that premalignant lesions possess many of the alterations found in cancer before the development of a malignant phenotype. Inactivation or mutation of the p53 tumor-suppressor might be an early event contributing to genomic instability and increasing the risk of malignant transformation.
T2  - European Journal of Oral Sciences
T1  - Genomic instability and tumor-specific DNA alterations in oral leukoplakias
VL  - 117
IS  - 3
SP  - 231
EP  - 237
DO  - 10.1111/j.1600-0722.2009.00624.x
ER  - 

@article{
author = "Tanić, Nikola and Tanić, Nikola and Milašin, Jelena and Vukadinovic, Miroslav and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "Leukoplakias, clinically identifiable premalignant lesions, often precede oral squamous cell carcinoma (OSCC). Identification of leukoplakias that have the potential for transformation to malignancy is a key clinical problem. The aim of this study was to assess genomic instability, and to detect tumor-specific genomic alterations, in leukoplakias. Genomic instability was analyzed by comparing the DNA fingerprints of 32 leukoplakias with those of paired normal tissue. In addition, the mutational status of the p53 gene was analyzed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex DNA (PCR-HET), and the mutations were subsequently confirmed by DNA sequencing. Moderate-to-significant genomic instability was detected in all leukoplakias analysed. Nine unique amplicons, present in leukoplakias but not in normal tissue, were retrieved and successfully characterized. The p53 gene was mutated in 40.6% of patients. Four patients with moderate instability and mutated p53 developed OSCC. The data obtained in this study support and concretize the thesis that premalignant lesions possess many of the alterations found in cancer before the development of a malignant phenotype. Inactivation or mutation of the p53 tumor-suppressor might be an early event contributing to genomic instability and increasing the risk of malignant transformation.",
journal = "European Journal of Oral Sciences",
title = "Genomic instability and tumor-specific DNA alterations in oral leukoplakias",
volume = "117",
number = "3",
pages = "231-237",
doi = "10.1111/j.1600-0722.2009.00624.x"
}

Tanić, N., Tanić, N., Milašin, J., Vukadinovic, M.,& Dimitrijević, B. B.. (2009). Genomic instability and tumor-specific DNA alterations in oral leukoplakias. in European Journal of Oral Sciences, 117(3), 231-237.
https://doi.org/10.1111/j.1600-0722.2009.00624.x

Tanić N, Tanić N, Milašin J, Vukadinovic M, Dimitrijević BB. Genomic instability and tumor-specific DNA alterations in oral leukoplakias. in European Journal of Oral Sciences. 2009;117(3):231-237.
doi:10.1111/j.1600-0722.2009.00624.x .

Tanić, Nikola, Tanić, Nikola, Milašin, Jelena, Vukadinovic, Miroslav, Dimitrijević, Bogomir B., "Genomic instability and tumor-specific DNA alterations in oral leukoplakias" in European Journal of Oral Sciences, 117, no. 3 (2009):231-237,
https://doi.org/10.1111/j.1600-0722.2009.00624.x . .

TY  - JOUR
AU  - Papp, J
AU  - Raicevic, L
AU  - Milašin, Jelena
AU  - Dimitrijević, Bogomir B.
AU  - Radulović, Siniša S.
AU  - Olah, E
PY  - 1999
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2281
AB  - The frequency of germline BRCA1 and BRCA2 mutations was tested in Yugoslav breast and breast/ovarian cancer families using combined heteroduplex/single-strand conformation polymorphism analysis for the entire coding region of both genes. Three different recurrent BRCA1 mutations (one 185delAG, one 3447del4 and two 5382insC) were identified in 4 of 12 families (33%), whereas no definite disease-causing alterations of BRCA2 was detected. Genotype analysis revealed a possible common founder effect for each 185delAG and 5382insC. The relatively high frequency of germline BRCA1 mutations determined in this panel of families confirms the important role of BRCA1 in disease predisposition in the Yugoslav population, while the lack of population specific founder and/or unique mutations show the need of further analysis of samples from this yet unexamined region of Europe.
T2  - Oncology Reports
T1  - Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families
VL  - 6
IS  - 6
SP  - 1435
EP  - 1438
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2281
ER  - 

@article{
author = "Papp, J and Raicevic, L and Milašin, Jelena and Dimitrijević, Bogomir B. and Radulović, Siniša S. and Olah, E",
year = "1999",
abstract = "The frequency of germline BRCA1 and BRCA2 mutations was tested in Yugoslav breast and breast/ovarian cancer families using combined heteroduplex/single-strand conformation polymorphism analysis for the entire coding region of both genes. Three different recurrent BRCA1 mutations (one 185delAG, one 3447del4 and two 5382insC) were identified in 4 of 12 families (33%), whereas no definite disease-causing alterations of BRCA2 was detected. Genotype analysis revealed a possible common founder effect for each 185delAG and 5382insC. The relatively high frequency of germline BRCA1 mutations determined in this panel of families confirms the important role of BRCA1 in disease predisposition in the Yugoslav population, while the lack of population specific founder and/or unique mutations show the need of further analysis of samples from this yet unexamined region of Europe.",
journal = "Oncology Reports",
title = "Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families",
volume = "6",
number = "6",
pages = "1435-1438",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2281"
}

Papp, J., Raicevic, L., Milašin, J., Dimitrijević, B. B., Radulović, S. S.,& Olah, E.. (1999). Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families. in Oncology Reports, 6(6), 1435-1438.
https://hdl.handle.net/21.15107/rcub_vinar_2281

Papp J, Raicevic L, Milašin J, Dimitrijević BB, Radulović SS, Olah E. Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families. in Oncology Reports. 1999;6(6):1435-1438.
https://hdl.handle.net/21.15107/rcub_vinar_2281 .

Papp, J, Raicevic, L, Milašin, Jelena, Dimitrijević, Bogomir B., Radulović, Siniša S., Olah, E, "Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families" in Oncology Reports, 6, no. 6 (1999):1435-1438,
https://hdl.handle.net/21.15107/rcub_vinar_2281 .

TY  - JOUR
AU  - Milašin, Jelena
AU  - Petrović, Vlastimir
AU  - Nikolić, Živorad
AU  - Dedović, Nasta
AU  - Pujić, Natalija
AU  - Vranić, Vesna
PY  - 1993
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9511
AB  - Tačkaste mutacije RAS protoonkogena dovode do njihove aktivacije čime ovi geni stiču sposobnost indukovanja maligne transformacije. Učestalost mutiranih RAS gena veoma varira od jednog tipa do drugog, a podatak o učestalosti i značaju RAS mutacija u karcinomima usne nije nađe u literaturi. Koristeći veoma osetljivu metodu hibridizacije sa sintetičkim oligonukleotidnim probama, analizovana je DNK iz 9 uzoraka planocelularnog karcinoma usne i 6 kontrolnih uzoraka. U 5 uzoraka tumorskog tkiva detektovane su mutacije H-RAS gena, što predstavlja visoku učestalost i ukazuje na značaj njihove aktivacije u nastanku kancera usne.
T2  - Stomatološki glasnik Srbije
T1  - Učestalost aktivacije RAS onkogena u planocelularnih karcinoma donje usne
T1  - Activation of frequency of RAS oncogenes in planocellular carcinoma of the lower lip
VL  - 40
IS  - 1
SP  - 7
EP  - 10
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9511
ER  - 

@article{
author = "Milašin, Jelena and Petrović, Vlastimir and Nikolić, Živorad and Dedović, Nasta and Pujić, Natalija and Vranić, Vesna",
year = "1993",
abstract = "Tačkaste mutacije RAS protoonkogena dovode do njihove aktivacije čime ovi geni stiču sposobnost indukovanja maligne transformacije. Učestalost mutiranih RAS gena veoma varira od jednog tipa do drugog, a podatak o učestalosti i značaju RAS mutacija u karcinomima usne nije nađe u literaturi. Koristeći veoma osetljivu metodu hibridizacije sa sintetičkim oligonukleotidnim probama, analizovana je DNK iz 9 uzoraka planocelularnog karcinoma usne i 6 kontrolnih uzoraka. U 5 uzoraka tumorskog tkiva detektovane su mutacije H-RAS gena, što predstavlja visoku učestalost i ukazuje na značaj njihove aktivacije u nastanku kancera usne.",
journal = "Stomatološki glasnik Srbije",
title = "Učestalost aktivacije RAS onkogena u planocelularnih karcinoma donje usne, Activation of frequency of RAS oncogenes in planocellular carcinoma of the lower lip",
volume = "40",
number = "1",
pages = "7-10",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9511"
}

Milašin, J., Petrović, V., Nikolić, Ž., Dedović, N., Pujić, N.,& Vranić, V.. (1993). Učestalost aktivacije RAS onkogena u planocelularnih karcinoma donje usne. in Stomatološki glasnik Srbije, 40(1), 7-10.
https://hdl.handle.net/21.15107/rcub_vinar_9511

Milašin J, Petrović V, Nikolić Ž, Dedović N, Pujić N, Vranić V. Učestalost aktivacije RAS onkogena u planocelularnih karcinoma donje usne. in Stomatološki glasnik Srbije. 1993;40(1):7-10.
https://hdl.handle.net/21.15107/rcub_vinar_9511 .

Milašin, Jelena, Petrović, Vlastimir, Nikolić, Živorad, Dedović, Nasta, Pujić, Natalija, Vranić, Vesna, "Učestalost aktivacije RAS onkogena u planocelularnih karcinoma donje usne" in Stomatološki glasnik Srbije, 40, no. 1 (1993):7-10,
https://hdl.handle.net/21.15107/rcub_vinar_9511 .

TY  - JOUR
AU  - Milašin, Jelena
AU  - Dedović, Nasta
AU  - Pujić, Natalija
AU  - Vranić, Vesna
AU  - Mićić, S.
AU  - Dimitrijević, Bogomir B.
AU  - Diklić, V.
PY  - 1992
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9512
AB  - DNK, izolovana iz 32 tumora bešike, analizirana je na prisustvo tačkastih mutacija H-ras gena za koji se zna da učestvuje u razvitku različitih malignih tumora čoveka. Primenom tehnike hibridizacije oligonuklotidnim probama na in vitro umnoženu tumorsku DNK, tačkaste mutacije na 12. i 13. kodonu H-ras gena detektovane su u 7 od 32 tumorska uzorka (22%). Ovako niska učestalost mutacija H-ras gena upućuje na zaključak da njihova aktivacija nije dominantni genetski događaj u razvitku tumora bešike, bar ne u većem delu te tumorske populacije.
AB  - The DNA from thirty-two bladder cancers was screened for the presence of H-ras gene mutations, known to be involved in the pathogenesis of various human malignancies. Using the technique of oligonucleotide hybridization on previously in vitro amplified tumor DNA, H-ras mutations at codon 12 and 13 were detected in 7 out of 32 tumor specimens (22%). This low incidence of mutated ras genes suggests that their activation does not represent the crucial event in bladder tumor development, at least in the major part of tumor population.
T2  - Archivum urologicum
T1  - Mutacije ras-gena u tumorima bešike
T1  - Ras gene mutations in bladder tumors
VL  - 14
IS  - 35/36
SP  - 35
EP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9512
ER  - 

@article{
author = "Milašin, Jelena and Dedović, Nasta and Pujić, Natalija and Vranić, Vesna and Mićić, S. and Dimitrijević, Bogomir B. and Diklić, V.",
year = "1992",
abstract = "DNK, izolovana iz 32 tumora bešike, analizirana je na prisustvo tačkastih mutacija H-ras gena za koji se zna da učestvuje u razvitku različitih malignih tumora čoveka. Primenom tehnike hibridizacije oligonuklotidnim probama na in vitro umnoženu tumorsku DNK, tačkaste mutacije na 12. i 13. kodonu H-ras gena detektovane su u 7 od 32 tumorska uzorka (22%). Ovako niska učestalost mutacija H-ras gena upućuje na zaključak da njihova aktivacija nije dominantni genetski događaj u razvitku tumora bešike, bar ne u većem delu te tumorske populacije., The DNA from thirty-two bladder cancers was screened for the presence of H-ras gene mutations, known to be involved in the pathogenesis of various human malignancies. Using the technique of oligonucleotide hybridization on previously in vitro amplified tumor DNA, H-ras mutations at codon 12 and 13 were detected in 7 out of 32 tumor specimens (22%). This low incidence of mutated ras genes suggests that their activation does not represent the crucial event in bladder tumor development, at least in the major part of tumor population.",
journal = "Archivum urologicum",
title = "Mutacije ras-gena u tumorima bešike, Ras gene mutations in bladder tumors",
volume = "14",
number = "35/36",
pages = "35-40",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9512"
}

Milašin, J., Dedović, N., Pujić, N., Vranić, V., Mićić, S., Dimitrijević, B. B.,& Diklić, V.. (1992). Mutacije ras-gena u tumorima bešike. in Archivum urologicum, 14(35/36), 35-40.
https://hdl.handle.net/21.15107/rcub_vinar_9512

Milašin J, Dedović N, Pujić N, Vranić V, Mićić S, Dimitrijević BB, Diklić V. Mutacije ras-gena u tumorima bešike. in Archivum urologicum. 1992;14(35/36):35-40.
https://hdl.handle.net/21.15107/rcub_vinar_9512 .

Milašin, Jelena, Dedović, Nasta, Pujić, Natalija, Vranić, Vesna, Mićić, S., Dimitrijević, Bogomir B., Diklić, V., "Mutacije ras-gena u tumorima bešike" in Archivum urologicum, 14, no. 35/36 (1992):35-40,
https://hdl.handle.net/21.15107/rcub_vinar_9512 .