TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Wu, Victoria
AU  - Ajduković, Zorica
AU  - Mihajilov-Krstev, Tatjana
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7570
AB  - Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues
VL  - 60
SP  - 357
EP  - 364
DO  - 10.1016/j.msec.2015.11.061
ER  - 

@article{
author = "Ignjatović, Nenad L. and Wu, Victoria and Ajduković, Zorica and Mihajilov-Krstev, Tatjana and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues",
volume = "60",
pages = "357-364",
doi = "10.1016/j.msec.2015.11.061"
}

Ignjatović, N. L., Wu, V., Ajduković, Z., Mihajilov-Krstev, T., Uskoković, V.,& Uskoković, D.. (2016). Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. in Materials Science and Engineering C
Elsevier., 60, 357-364.
https://doi.org/10.1016/j.msec.2015.11.061

Ignjatović NL, Wu V, Ajduković Z, Mihajilov-Krstev T, Uskoković V, Uskoković D. Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. in Materials Science and Engineering C. 2016;60:357-364.
doi:10.1016/j.msec.2015.11.061 .

Ignjatović, Nenad L., Wu, Victoria, Ajduković, Zorica, Mihajilov-Krstev, Tatjana, Uskoković, Vuk, Uskoković, Dragan, "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues" in Materials Science and Engineering C, 60 (2016):357-364,
https://doi.org/10.1016/j.msec.2015.11.061 . .

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Wu, Victoria
AU  - Ajduković, Zorica
AU  - Mihajilov Krstev, Tatjana
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7573
AB  - Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.
T2  - Materials science & engineering. C, Materials for biological applications
T1  - Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues
VL  - 60
SP  - 357
EP  - 364
DO  - 10.1016/j.msec.2015.11.061
ER  - 

@article{
author = "Ignjatović, Nenad L. and Wu, Victoria and Ajduković, Zorica and Mihajilov Krstev, Tatjana and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.",
journal = "Materials science & engineering. C, Materials for biological applications",
title = "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues",
volume = "60",
pages = "357-364",
doi = "10.1016/j.msec.2015.11.061"
}

Ignjatović, N. L., Wu, V., Ajduković, Z., Mihajilov Krstev, T., Uskoković, V.,& Uskoković, D.. (2016). Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. in Materials science & engineering. C, Materials for biological applications, 60, 357-364.
https://doi.org/10.1016/j.msec.2015.11.061

Ignjatović NL, Wu V, Ajduković Z, Mihajilov Krstev T, Uskoković V, Uskoković D. Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. in Materials science & engineering. C, Materials for biological applications. 2016;60:357-364.
doi:10.1016/j.msec.2015.11.061 .

Ignjatović, Nenad L., Wu, Victoria, Ajduković, Zorica, Mihajilov Krstev, Tatjana, Uskoković, Vuk, Uskoković, Dragan, "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues" in Materials science & engineering. C, Materials for biological applications, 60 (2016):357-364,
https://doi.org/10.1016/j.msec.2015.11.061 . .

TY  - JOUR
AU  - Pejović, Anka
AU  - Stevanović, Dragana D.
AU  - Damljanović, Ivan S.
AU  - Vukićević, Mirjana
AU  - Novaković, Slađana B.
AU  - Bogdanović, Goran A.
AU  - Mihajilov-Krstev, Tatjana
AU  - Radulovic, Niko
AU  - Vukićević, Rastko D.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5014
AB  - A successful aza-Michael addition of arylamines to a conjugated enone, acryloylferrocene, has been achieved by ultrasonic irradiation of the mixture of these reactants and the catalyst, i.e., montmorillonite K-10. This solvent-free reaction, yielding ferrocene containing Mannich bases, 3-(arylamino)-1-ferrocenylpropan-1-ones, considered as valuable precursors in organic synthesis, has been performed by using a simple ultrasonic cleaner. Among 17 synthesized beta-amino ketones, three were new ones, and these were fully characterized by spectroscopic means. X-Ray crystallographic analysis of three of these crystalline products enabled the insight into the conformational details of these compounds. All compounds were evaluated for their antibacterial activities against six Gram-positive and five Gram-negative strains in a microdilution assay. The observed promising antibacterial activity (with a MIC value of 25 mu g/ml (ca. 0.07 mu mol/ml) as the best result for almost all tested compounds against Micrococcus flavus) seems not only to be compound but also bacterial species-specific.
T2  - Helvetica Chimica Acta
T1  - Ultrasound-Assisted Synthesis of 3-(Arylamino)-1-ferrocenylpropan-1-ones
VL  - 95
IS  - 8
SP  - 1425
EP  - 1441
DO  - 10.1002/hlca.201200009
ER  - 

@article{
author = "Pejović, Anka and Stevanović, Dragana D. and Damljanović, Ivan S. and Vukićević, Mirjana and Novaković, Slađana B. and Bogdanović, Goran A. and Mihajilov-Krstev, Tatjana and Radulovic, Niko and Vukićević, Rastko D.",
year = "2012",
abstract = "A successful aza-Michael addition of arylamines to a conjugated enone, acryloylferrocene, has been achieved by ultrasonic irradiation of the mixture of these reactants and the catalyst, i.e., montmorillonite K-10. This solvent-free reaction, yielding ferrocene containing Mannich bases, 3-(arylamino)-1-ferrocenylpropan-1-ones, considered as valuable precursors in organic synthesis, has been performed by using a simple ultrasonic cleaner. Among 17 synthesized beta-amino ketones, three were new ones, and these were fully characterized by spectroscopic means. X-Ray crystallographic analysis of three of these crystalline products enabled the insight into the conformational details of these compounds. All compounds were evaluated for their antibacterial activities against six Gram-positive and five Gram-negative strains in a microdilution assay. The observed promising antibacterial activity (with a MIC value of 25 mu g/ml (ca. 0.07 mu mol/ml) as the best result for almost all tested compounds against Micrococcus flavus) seems not only to be compound but also bacterial species-specific.",
journal = "Helvetica Chimica Acta",
title = "Ultrasound-Assisted Synthesis of 3-(Arylamino)-1-ferrocenylpropan-1-ones",
volume = "95",
number = "8",
pages = "1425-1441",
doi = "10.1002/hlca.201200009"
}

Pejović, A., Stevanović, D. D., Damljanović, I. S., Vukićević, M., Novaković, S. B., Bogdanović, G. A., Mihajilov-Krstev, T., Radulovic, N.,& Vukićević, R. D.. (2012). Ultrasound-Assisted Synthesis of 3-(Arylamino)-1-ferrocenylpropan-1-ones. in Helvetica Chimica Acta, 95(8), 1425-1441.
https://doi.org/10.1002/hlca.201200009

Pejović A, Stevanović DD, Damljanović IS, Vukićević M, Novaković SB, Bogdanović GA, Mihajilov-Krstev T, Radulovic N, Vukićević RD. Ultrasound-Assisted Synthesis of 3-(Arylamino)-1-ferrocenylpropan-1-ones. in Helvetica Chimica Acta. 2012;95(8):1425-1441.
doi:10.1002/hlca.201200009 .

Pejović, Anka, Stevanović, Dragana D., Damljanović, Ivan S., Vukićević, Mirjana, Novaković, Slađana B., Bogdanović, Goran A., Mihajilov-Krstev, Tatjana, Radulovic, Niko, Vukićević, Rastko D., "Ultrasound-Assisted Synthesis of 3-(Arylamino)-1-ferrocenylpropan-1-ones" in Helvetica Chimica Acta, 95, no. 8 (2012):1425-1441,
https://doi.org/10.1002/hlca.201200009 . .