TY  - JOUR
AU  - Nunes, Nadia
AU  - Popović, Iva
AU  - Abreu, Elder
AU  - Maciel, Dina
AU  - Rodrigues, João
AU  - Soto, Juan
AU  - Algarra, Manuel
AU  - Petković, Marijana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13317
AB  - We studied the possibility of detection of [Ru(η5-C5H5)(PPh3)2Cl] (abbreviated by RuCp) complex as a model system for Ru-based metallodrugs in human urine by using matrix-assisted laser desorption/ionization time-of- flight mass spectrometry (MALDI-TOF MS) without previous purification or removal of inorganic salts. Inorganic salts might prevent the detection of RuCp by MALDI-TOF MS, most likely through the increased number and intensity of background/organic matrix signals. This problem might be overcome by the acquisition of matrix- free spectra and the addition of nanoparticles, such as carbon dots, to the urine solution. Our results suggest that RuCp is easily detectable by MALDI-TOF MS in all acquisition conditions, with the CHCA matrix being the best for acquisition in phosphate-containing solutions, whereas in urine, DHB and matrix-free approach demonstrated the highest sensitivity, precision, and reproducibility. The sensitivity of matrix-free MALDI detection of RuCp could be increased by the addition of carbon dots to the urine. Based on theoretical calculations for all matrix/analyte combinations, the model for the interaction of RuCp with carbon dots was established, and higher sensitivity explained.
T2  - Talanta
T1  - Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity
VL  - 222
SP  - 121551
DO  - 10.1016/j.talanta.2020.121551
ER  - 

@article{
author = "Nunes, Nadia and Popović, Iva and Abreu, Elder and Maciel, Dina and Rodrigues, João and Soto, Juan and Algarra, Manuel and Petković, Marijana",
year = "2021",
abstract = "We studied the possibility of detection of [Ru(η5-C5H5)(PPh3)2Cl] (abbreviated by RuCp) complex as a model system for Ru-based metallodrugs in human urine by using matrix-assisted laser desorption/ionization time-of- flight mass spectrometry (MALDI-TOF MS) without previous purification or removal of inorganic salts. Inorganic salts might prevent the detection of RuCp by MALDI-TOF MS, most likely through the increased number and intensity of background/organic matrix signals. This problem might be overcome by the acquisition of matrix- free spectra and the addition of nanoparticles, such as carbon dots, to the urine solution. Our results suggest that RuCp is easily detectable by MALDI-TOF MS in all acquisition conditions, with the CHCA matrix being the best for acquisition in phosphate-containing solutions, whereas in urine, DHB and matrix-free approach demonstrated the highest sensitivity, precision, and reproducibility. The sensitivity of matrix-free MALDI detection of RuCp could be increased by the addition of carbon dots to the urine. Based on theoretical calculations for all matrix/analyte combinations, the model for the interaction of RuCp with carbon dots was established, and higher sensitivity explained.",
journal = "Talanta",
title = "Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity",
volume = "222",
pages = "121551",
doi = "10.1016/j.talanta.2020.121551"
}

Nunes, N., Popović, I., Abreu, E., Maciel, D., Rodrigues, J., Soto, J., Algarra, M.,& Petković, M.. (2021). Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity. in Talanta, 222, 121551.
https://doi.org/10.1016/j.talanta.2020.121551

Nunes N, Popović I, Abreu E, Maciel D, Rodrigues J, Soto J, Algarra M, Petković M. Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity. in Talanta. 2021;222:121551.
doi:10.1016/j.talanta.2020.121551 .

Nunes, Nadia, Popović, Iva, Abreu, Elder, Maciel, Dina, Rodrigues, João, Soto, Juan, Algarra, Manuel, Petković, Marijana, "Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity" in Talanta, 222 (2021):121551,
https://doi.org/10.1016/j.talanta.2020.121551 . .

TY  - JOUR
AU  - Houdová, Dominika
AU  - Soto, Juan
AU  - Castro, Rita
AU  - Rodrigues, João
AU  - Soledad Pino-González, Ma
AU  - Petković, Marijana
AU  - Bandosz, Teresa J
AU  - Algarra, Manuel
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9127
AB  - A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.
T2  - Journal of Colloid and Interface Science
T1  - Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry
VL  - 591
SP  - 373
EP  - 383
DO  - 10.1016/j.jcis.2021.02.004
ER  - 

@article{
author = "Houdová, Dominika and Soto, Juan and Castro, Rita and Rodrigues, João and Soledad Pino-González, Ma and Petković, Marijana and Bandosz, Teresa J and Algarra, Manuel",
year = "2021",
abstract = "A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.",
journal = "Journal of Colloid and Interface Science",
title = "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry",
volume = "591",
pages = "373-383",
doi = "10.1016/j.jcis.2021.02.004"
}

Houdová, D., Soto, J., Castro, R., Rodrigues, J., Soledad Pino-González, M., Petković, M., Bandosz, T. J.,& Algarra, M.. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science, 591, 373-383.
https://doi.org/10.1016/j.jcis.2021.02.004

Houdová D, Soto J, Castro R, Rodrigues J, Soledad Pino-González M, Petković M, Bandosz TJ, Algarra M. Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science. 2021;591:373-383.
doi:10.1016/j.jcis.2021.02.004 .

Houdová, Dominika, Soto, Juan, Castro, Rita, Rodrigues, João, Soledad Pino-González, Ma, Petković, Marijana, Bandosz, Teresa J, Algarra, Manuel, "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry" in Journal of Colloid and Interface Science, 591 (2021):373-383,
https://doi.org/10.1016/j.jcis.2021.02.004 . .

TY  - JOUR
AU  - Houdová, Dominika
AU  - Soto, Juan
AU  - Castro, Rita
AU  - Rodrigues, João
AU  - Soledad Pino-González, Ma
AU  - Petković, Marijana
AU  - Bandosz, Teresa J
AU  - Algarra, Manuel
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9145
AB  - A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.
T2  - Journal of Colloid and Interface Science
T1  - Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry
VL  - 591
SP  - 373
EP  - 383
DO  - 10.1016/j.jcis.2021.02.004
ER  - 

@article{
author = "Houdová, Dominika and Soto, Juan and Castro, Rita and Rodrigues, João and Soledad Pino-González, Ma and Petković, Marijana and Bandosz, Teresa J and Algarra, Manuel",
year = "2021",
abstract = "A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.",
journal = "Journal of Colloid and Interface Science",
title = "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry",
volume = "591",
pages = "373-383",
doi = "10.1016/j.jcis.2021.02.004"
}

Houdová, D., Soto, J., Castro, R., Rodrigues, J., Soledad Pino-González, M., Petković, M., Bandosz, T. J.,& Algarra, M.. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science, 591, 373-383.
https://doi.org/10.1016/j.jcis.2021.02.004

Houdová D, Soto J, Castro R, Rodrigues J, Soledad Pino-González M, Petković M, Bandosz TJ, Algarra M. Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science. 2021;591:373-383.
doi:10.1016/j.jcis.2021.02.004 .

Houdová, Dominika, Soto, Juan, Castro, Rita, Rodrigues, João, Soledad Pino-González, Ma, Petković, Marijana, Bandosz, Teresa J, Algarra, Manuel, "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry" in Journal of Colloid and Interface Science, 591 (2021):373-383,
https://doi.org/10.1016/j.jcis.2021.02.004 . .