Spremo-Potparević, Biljana

Link to this page

Authority KeyName Variants
orcid::0000-0001-5570-883X
  • Spremo-Potparević, Biljana (32)
Projects
Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell Hormonal regulation of expression and activity of the nitric oxide synthase and sodium-potassium pump in experimental models of insulin resistance, diabetes and cardiovascular disorders
COST Action [CA15132, ‘hCOMET’] Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion
KAUST Base Research Fund [BAS/1/1606-01-01] KAUST Office of Sponsored Research (OSR) [FCC/1/1976-17-01]
hCOMET COST action (No. 15132) Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation
Characterization and application of fungal metabolites and assessment of new biofungicides potential Molecular determinants for tumor marker design
Carotid disease in Serbia - pathologic dynamics, prevention, diagnostics and inovative therapeutic methods Evaluacija dejstva hormona i citostatika prmenom citogenetičkih analiza i Komet testa
Ecophysiological and genetic investigations of domestic animals and bees for the purpose of increasing reproductive traits and disease resistance KAUST [BAS/1/1059-01-01]
KAUST grant [OSR#4129] KAUST grant OSR [4129]
National Institutes of Health [AG028679, AG031364] National Science Foundation [CBET-1263994], Veterans Administration [5 I01 BX000418-06], Fulbright Program
Republic of Serbia [680-00-566/2013-09/02], Republic of Italy [680-00-566/2013-09/02] Serbian Ministry of Science and Technological Development [143022]

Author's Bibliography

The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”

Bajić, Vladan P.; Essack, Magbubah; Živković, Lada; Stewart, Alan J.; Zafirović, Sonja; Bajić, Vladimir B.; Gojobori, Takashi; Isenović, Esma R.; Spremo-Potparević, Biljana

(2020)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Essack, Magbubah
AU  - Živković, Lada
AU  - Stewart, Alan J.
AU  - Zafirović, Sonja
AU  - Bajić, Vladimir B.
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8825
AB  - Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics. © Copyright © 2020 Bajic, Essack, Zivkovic, Stewart, Zafirovic, Bajic, Gojobori, Isenovic and Spremo-Potparevic.
T2  - Frontiers in Genetics
T1  - The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”
VL  - 10
DO  - 10.3389/fgene.2019.01368
ER  - 
@article{
author = "Bajić, Vladan P. and Essack, Magbubah and Živković, Lada and Stewart, Alan J. and Zafirović, Sonja and Bajić, Vladimir B. and Gojobori, Takashi and Isenović, Esma R. and Spremo-Potparević, Biljana",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/8825",
abstract = "Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics. © Copyright © 2020 Bajic, Essack, Zivkovic, Stewart, Zafirovic, Bajic, Gojobori, Isenovic and Spremo-Potparevic.",
journal = "Frontiers in Genetics",
title = "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”",
volume = "10",
doi = "10.3389/fgene.2019.01368"
}
Bajić, V. P., Essack, M., Živković, L., Stewart, A. J., Zafirović, S., Bajić, V. B., Gojobori, T., Isenović, E. R.,& Spremo-Potparević, B. (2020). The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”.
Frontiers in Genetics, 10.
https://doi.org/10.3389/fgene.2019.01368
Bajić VP, Essack M, Živković L, Stewart AJ, Zafirović S, Bajić VB, Gojobori T, Isenović ER, Spremo-Potparević B. The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. Frontiers in Genetics. 2020;10
Bajić Vladan P., Essack Magbubah, Živković Lada, Stewart Alan J., Zafirović Sonja, Bajić Vladimir B., Gojobori Takashi, Isenović Esma R., Spremo-Potparević Biljana, "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”" Frontiers in Genetics, 10 (2020),
https://doi.org/10.3389/fgene.2019.01368 .
13
5
2
2

Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy

Obradović, Milan M.; Zafirović, Sonja; Essack, Magbubah; Dimitrov, Jelena; Živković, Lada; Spremo-Potparević, Biljana; Radak, Đorđe J.; Bajić, Vladimir B.; Isenović, Esma R.

(Churchill Livingstone, 2020)

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Essack, Magbubah
AU  - Dimitrov, Jelena
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Radak, Đorđe J.
AU  - Bajić, Vladimir B.
AU  - Isenović, Esma R.
PY  - 2020
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8487
AB  - To remedy carotid artery stenosis and prevent stroke surgical intervention is commonly used, and the gold standard being carotid endarterectomy (CEA). During CEA cerebrovascular hemoglobin oxygen saturation decreases and when this decrease reaches critical levels it leads to cerebral hypoxia that causes neuronal damage. One of the proposed mechanism that affects changes during CEA and contribute to acute brain ischemia (ABI) is oxidative stress. The increased production of reactive oxygen species and reactive nitrogen species during ABI may cause an unregulated inflammatory response and further lead to structural and functional injury of neurons. Antioxidant activity are involved in the protection against neuronal damage after cerebral ischemia. We hypothesized that neuronal injury and poor outcomes in patients undergoing CEA may be results of oxidative stress that disturbed function of antioxidant enzymes and contributed to the DNA damage in lymphocytes. © 2019 The Authors
PB  - Churchill Livingstone
T2  - Medical Hypotheses
T1  - Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy
VL  - 134
SP  - 109419
DO  - 10.1016/j.mehy.2019.109419
ER  - 
@article{
author = "Obradović, Milan M. and Zafirović, Sonja and Essack, Magbubah and Dimitrov, Jelena and Živković, Lada and Spremo-Potparević, Biljana and Radak, Đorđe J. and Bajić, Vladimir B. and Isenović, Esma R.",
year = "2020",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8487",
abstract = "To remedy carotid artery stenosis and prevent stroke surgical intervention is commonly used, and the gold standard being carotid endarterectomy (CEA). During CEA cerebrovascular hemoglobin oxygen saturation decreases and when this decrease reaches critical levels it leads to cerebral hypoxia that causes neuronal damage. One of the proposed mechanism that affects changes during CEA and contribute to acute brain ischemia (ABI) is oxidative stress. The increased production of reactive oxygen species and reactive nitrogen species during ABI may cause an unregulated inflammatory response and further lead to structural and functional injury of neurons. Antioxidant activity are involved in the protection against neuronal damage after cerebral ischemia. We hypothesized that neuronal injury and poor outcomes in patients undergoing CEA may be results of oxidative stress that disturbed function of antioxidant enzymes and contributed to the DNA damage in lymphocytes. © 2019 The Authors",
publisher = "Churchill Livingstone",
journal = "Medical Hypotheses",
title = "Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy",
volume = "134",
pages = "109419",
doi = "10.1016/j.mehy.2019.109419"
}
Obradović, M. M., Zafirović, S., Essack, M., Dimitrov, J., Živković, L., Spremo-Potparević, B., Radak, Đ. J., Bajić, V. B.,& Isenović, E. R. (2020). Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy.
Medical Hypotheses
Churchill Livingstone., 134, 109419.
https://doi.org/10.1016/j.mehy.2019.109419
Obradović MM, Zafirović S, Essack M, Dimitrov J, Živković L, Spremo-Potparević B, Radak ĐJ, Bajić VB, Isenović ER. Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy. Medical Hypotheses. 2020;134:109419
Obradović Milan M., Zafirović Sonja, Essack Magbubah, Dimitrov Jelena, Živković Lada, Spremo-Potparević Biljana, Radak Đorđe J., Bajić Vladimir B., Isenović Esma R., "Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy" Medical Hypotheses, 134 (2020):109419,
https://doi.org/10.1016/j.mehy.2019.109419 .
1

Evaluation of antioxidant potential of Cordyceps sinensis in vitro

Živković, Lada; Borozan, Sunčica; Bajić, Vladan P.; Đorđević, Stefana; Hristov, Aleksandar; Spremo-Potparević, Biljana

(2019)

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Bajić, Vladan P.
AU  - Đorđević, Stefana
AU  - Hristov, Aleksandar
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9002
AB  - Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078-2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078-10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers.
AB  - Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) - skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.
T2  - Medicinski časopis
T1  - Evaluation of antioxidant potential of Cordyceps sinensis in vitro
T1  - Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro
VL  - 53
IS  - 4
SP  - 129
EP  - 134
DO  - 10.5937/mckg53-24450
ER  - 
@article{
author = "Živković, Lada and Borozan, Sunčica and Bajić, Vladan P. and Đorđević, Stefana and Hristov, Aleksandar and Spremo-Potparević, Biljana",
year = "2019",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/9002",
abstract = "Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078-2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078-10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers., Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) - skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.",
journal = "Medicinski časopis",
title = "Evaluation of antioxidant potential of Cordyceps sinensis in vitro, Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro",
volume = "53",
number = "4",
pages = "129-134",
doi = "10.5937/mckg53-24450"
}
Živković, L., Borozan, S., Bajić, V. P., Đorđević, S., Hristov, A.,& Spremo-Potparević, B. (2019). Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro.
Medicinski časopis, 53(4), 129-134.
https://doi.org/10.5937/mckg53-24450
Živković L, Borozan S, Bajić VP, Đorđević S, Hristov A, Spremo-Potparević B. Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro. Medicinski časopis. 2019;53(4):129-134
Živković Lada, Borozan Sunčica, Bajić Vladan P., Đorđević Stefana, Hristov Aleksandar, Spremo-Potparević Biljana, "Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro" Medicinski časopis, 53, no. 4 (2019):129-134,
https://doi.org/10.5937/mckg53-24450 .

Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells

Živković, Lada; Bajić, Vladan P.; Topalović, Dijana; Bruić, Marija; Spremo-Potparević, Biljana

(2019)

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan P.
AU  - Topalović, Dijana
AU  - Bruić, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8666
AB  - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μ g/mL, 500 μ g/mL and 1000 μ g/mL) and dihydroquercetin (100 μ g/mL, 250 μ g/mL and 500 μ g/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H 2 O 2 -) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H 2 O 2 -induced damage on DNA in cells at all tested concentrations, with a statistical significance ( p < 0.05 ), whereas Biochaga at the dose of 500 μ g/mL in combination with dihydroquercetin 500 μ g/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro .
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells
VL  - 2019
SP  - 5039372
DO  - 10.1155/2019/5039372
ER  - 
@article{
author = "Živković, Lada and Bajić, Vladan P. and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8666",
abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μ g/mL, 500 μ g/mL and 1000 μ g/mL) and dihydroquercetin (100 μ g/mL, 250 μ g/mL and 500 μ g/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H 2 O 2 -) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H 2 O 2 -induced damage on DNA in cells at all tested concentrations, with a statistical significance ( p < 0.05 ), whereas Biochaga at the dose of 500 μ g/mL in combination with dihydroquercetin 500 μ g/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro .",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells",
volume = "2019",
pages = "5039372",
doi = "10.1155/2019/5039372"
}
Živković, L., Bajić, V. P., Topalović, D., Bruić, M.,& Spremo-Potparević, B. (2019). Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells.
Oxidative Medicine and Cellular Longevity, 2019, 5039372.
https://doi.org/10.1155/2019/5039372
Živković L, Bajić VP, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. Oxidative Medicine and Cellular Longevity. 2019;2019:5039372
Živković Lada, Bajić Vladan P., Topalović Dijana, Bruić Marija, Spremo-Potparević Biljana, "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells" Oxidative Medicine and Cellular Longevity, 2019 (2019):5039372,
https://doi.org/10.1155/2019/5039372 .
3
1
2

Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro

Živković, Lada; Bajić, Vladan P.; Dekanski, Dragana; Čabarkapa-Pirković, Andrea; Giampieri, Francesca; Gasparrini, Massimiliano; Mazzoni, Luca; Spremo-Potparević, Biljana

(2018)

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan P.
AU  - Dekanski, Dragana
AU  - Čabarkapa-Pirković, Andrea
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Mazzoni, Luca
AU  - Spremo-Potparević, Biljana
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S027869151830334X
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7819
AB  - Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.
T2  - Food and Chemical Toxicology
T1  - Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro
VL  - 119
SP  - 61
EP  - 65
DO  - 10.1016/j.fct.2018.05.034
ER  - 
@article{
author = "Živković, Lada and Bajić, Vladan P. and Dekanski, Dragana and Čabarkapa-Pirković, Andrea and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Spremo-Potparević, Biljana",
year = "2018",
url = "https://linkinghub.elsevier.com/retrieve/pii/S027869151830334X, http://vinar.vin.bg.ac.rs/handle/123456789/7819",
abstract = "Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.",
journal = "Food and Chemical Toxicology",
title = "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro",
volume = "119",
pages = "61-65",
doi = "10.1016/j.fct.2018.05.034"
}
Živković, L., Bajić, V. P., Dekanski, D., Čabarkapa-Pirković, A., Giampieri, F., Gasparrini, M., Mazzoni, L.,& Spremo-Potparević, B. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro.
Food and Chemical Toxicology, 119, 61-65.
https://doi.org/10.1016/j.fct.2018.05.034
Živković L, Bajić VP, Dekanski D, Čabarkapa-Pirković A, Giampieri F, Gasparrini M, Mazzoni L, Spremo-Potparević B. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. Food and Chemical Toxicology. 2018;119:61-65
Živković Lada, Bajić Vladan P., Dekanski Dragana, Čabarkapa-Pirković Andrea, Giampieri Francesca, Gasparrini Massimiliano, Mazzoni Luca, Spremo-Potparević Biljana, "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro" Food and Chemical Toxicology, 119 (2018):61-65,
https://doi.org/10.1016/j.fct.2018.05.034 .
8
5
6

Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid

Dekanski, Dragana; Spremo-Potparević, Biljana; Bajić, Vladan P.; Živković, Lada; Topalović, Dijana; Sredojević, Dušan; Lazić, Vesna M.; Nedeljković, Jovan

(2018)

TY  - JOUR
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan P.
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Sredojević, Dušan
AU  - Lazić, Vesna M.
AU  - Nedeljković, Jovan
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0278691518301388
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7790
AB  - The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.
T2  - Food and Chemical Toxicology
T1  - Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid
VL  - 115
SP  - 42
EP  - 48
DO  - 10.1016/j.fct.2018.02.064
ER  - 
@article{
author = "Dekanski, Dragana and Spremo-Potparević, Biljana and Bajić, Vladan P. and Živković, Lada and Topalović, Dijana and Sredojević, Dušan and Lazić, Vesna M. and Nedeljković, Jovan",
year = "2018",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0278691518301388, http://vinar.vin.bg.ac.rs/handle/123456789/7790",
abstract = "The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.",
journal = "Food and Chemical Toxicology",
title = "Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid",
volume = "115",
pages = "42-48",
doi = "10.1016/j.fct.2018.02.064"
}
Dekanski, D., Spremo-Potparević, B., Bajić, V. P., Živković, L., Topalović, D., Sredojević, D., Lazić, V. M.,& Nedeljković, J. (2018). Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid.
Food and Chemical Toxicology, 115, 42-48.
https://doi.org/10.1016/j.fct.2018.02.064
Dekanski D, Spremo-Potparević B, Bajić VP, Živković L, Topalović D, Sredojević D, Lazić VM, Nedeljković J. Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid. Food and Chemical Toxicology. 2018;115:42-48
Dekanski Dragana, Spremo-Potparević Biljana, Bajić Vladan P., Živković Lada, Topalović Dijana, Sredojević Dušan, Lazić Vesna M., Nedeljković Jovan, "Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid" Food and Chemical Toxicology, 115 (2018):42-48,
https://doi.org/10.1016/j.fct.2018.02.064 .
11
11
13

Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres

Živković, Lada; Akar, Banu; Roux, Brianna M.; Spremo-Potparević, Biljana; Bajić, Vladan P.; Brey, Eric M.

(2017)

TY  - JOUR
AU  - Živković, Lada
AU  - Akar, Banu
AU  - Roux, Brianna M.
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan P.
AU  - Brey, Eric M.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1324
AB  - Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 284-291, 2017.
T2  - Journal of Biomedical Materials Research. Part A
T1  - Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres
VL  - 105
IS  - 1
SP  - 284
EP  - 291
DO  - 10.1002/jbm.a.35849
ER  - 
@article{
author = "Živković, Lada and Akar, Banu and Roux, Brianna M. and Spremo-Potparević, Biljana and Bajić, Vladan P. and Brey, Eric M.",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1324",
abstract = "Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 284-291, 2017.",
journal = "Journal of Biomedical Materials Research. Part A",
title = "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres",
volume = "105",
number = "1",
pages = "284-291",
doi = "10.1002/jbm.a.35849"
}
Živković, L., Akar, B., Roux, B. M., Spremo-Potparević, B., Bajić, V. P.,& Brey, E. M. (2017). Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres.
Journal of Biomedical Materials Research. Part A, 105(1), 284-291.
https://doi.org/10.1002/jbm.a.35849
Živković L, Akar B, Roux BM, Spremo-Potparević B, Bajić VP, Brey EM. Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. Journal of Biomedical Materials Research. Part A. 2017;105(1):284-291
Živković Lada, Akar Banu, Roux Brianna M., Spremo-Potparević Biljana, Bajić Vladan P., Brey Eric M., "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres" Journal of Biomedical Materials Research. Part A, 105, no. 1 (2017):284-291,
https://doi.org/10.1002/jbm.a.35849 .
1
2
3
4

Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro

Bajić, Vladan P.; Spremo-Potparević, Biljana; Živković, Lada; Cabarkapa, Andrea; Kotur-Stevuljevic, Jelena; Isenović, Esma R.; Sredojević, Dušan; Vukoje, Ivana D.; Lazić, Vesna M.; Ahrenkiel, Scott Phillip; Nedeljković, Jovan

(2017)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Cabarkapa, Andrea
AU  - Kotur-Stevuljevic, Jelena
AU  - Isenović, Esma R.
AU  - Sredojević, Dušan
AU  - Vukoje, Ivana D.
AU  - Lazić, Vesna M.
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1607
AB  - The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Jobs method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro
VL  - 155
SP  - 323
EP  - 331
DO  - 10.1016/j.colsurfb.2017.04.032
ER  - 
@article{
author = "Bajić, Vladan P. and Spremo-Potparević, Biljana and Živković, Lada and Cabarkapa, Andrea and Kotur-Stevuljevic, Jelena and Isenović, Esma R. and Sredojević, Dušan and Vukoje, Ivana D. and Lazić, Vesna M. and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1607",
abstract = "The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Jobs method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro",
volume = "155",
pages = "323-331",
doi = "10.1016/j.colsurfb.2017.04.032"
}
Bajić, V. P., Spremo-Potparević, B., Živković, L., Cabarkapa, A., Kotur-Stevuljevic, J., Isenović, E. R., Sredojević, D., Vukoje, I. D., Lazić, V. M., Ahrenkiel, S. P.,& Nedeljković, J. (2017). Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro.
Colloids and Surfaces. B: Biointerfaces, 155, 323-331.
https://doi.org/10.1016/j.colsurfb.2017.04.032
Bajić VP, Spremo-Potparević B, Živković L, Cabarkapa A, Kotur-Stevuljevic J, Isenović ER, Sredojević D, Vukoje ID, Lazić VM, Ahrenkiel SP, Nedeljković J. Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. Colloids and Surfaces. B: Biointerfaces. 2017;155:323-331
Bajić Vladan P., Spremo-Potparević Biljana, Živković Lada, Cabarkapa Andrea, Kotur-Stevuljevic Jelena, Isenović Esma R., Sredojević Dušan, Vukoje Ivana D., Lazić Vesna M., Ahrenkiel Scott Phillip, Nedeljković Jovan, "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro" Colloids and Surfaces. B: Biointerfaces, 155 (2017):323-331,
https://doi.org/10.1016/j.colsurfb.2017.04.032 .
14
15
17

Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells

Živković, Lada; Borozan, Sunčica Z.; Cabarkapa, Andrea; Topalović, Dijana; Ciptasari, Ummi; Bajić, Vladan P.; Spremo-Potparević, Biljana

(2017)

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Cabarkapa, Andrea
AU  - Topalović, Dijana
AU  - Ciptasari, Ummi
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1458
AB  - The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells
DO  - 10.1155/2017/8759764
ER  - 
@article{
author = "Živković, Lada and Borozan, Sunčica Z. and Cabarkapa, Andrea and Topalović, Dijana and Ciptasari, Ummi and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1458",
abstract = "The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells",
doi = "10.1155/2017/8759764"
}
Živković, L., Borozan, S. Z., Cabarkapa, A., Topalović, D., Ciptasari, U., Bajić, V. P.,& Spremo-Potparević, B. (2017). Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells.
Oxidative Medicine and Cellular Longevity.
https://doi.org/10.1155/2017/8759764
Živković L, Borozan SZ, Cabarkapa A, Topalović D, Ciptasari U, Bajić VP, Spremo-Potparević B. Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. Oxidative Medicine and Cellular Longevity. 2017;
Živković Lada, Borozan Sunčica Z., Cabarkapa Andrea, Topalović Dijana, Ciptasari Ummi, Bajić Vladan P., Spremo-Potparević Biljana, "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells" Oxidative Medicine and Cellular Longevity (2017),
https://doi.org/10.1155/2017/8759764 .
12
11
15

Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy

Cabarkapa, Andrea; Dekanski, Dragana; Živković, Lada; Milanovic-Cabarkapa, Mirjana; Bajić, Vladan P.; Topalović, Dijana; Giampieri, Francesca; Gasparrini, Massimiliano; Battino, Maurizio; Spremo-Potparević, Biljana

(2017)

TY  - JOUR
AU  - Cabarkapa, Andrea
AU  - Dekanski, Dragana
AU  - Živković, Lada
AU  - Milanovic-Cabarkapa, Mirjana
AU  - Bajić, Vladan P.
AU  - Topalović, Dijana
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Battino, Maurizio
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1679
AB  - The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy
VL  - 106
SP  - 616
EP  - 623
DO  - 10.1016/j.fct.2016.12.023
ER  - 
@article{
author = "Cabarkapa, Andrea and Dekanski, Dragana and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Bajić, Vladan P. and Topalović, Dijana and Giampieri, Francesca and Gasparrini, Massimiliano and Battino, Maurizio and Spremo-Potparević, Biljana",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1679",
abstract = "The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy",
volume = "106",
pages = "616-623",
doi = "10.1016/j.fct.2016.12.023"
}
Cabarkapa, A., Dekanski, D., Živković, L., Milanovic-Cabarkapa, M., Bajić, V. P., Topalović, D., Giampieri, F., Gasparrini, M., Battino, M.,& Spremo-Potparević, B. (2017). Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy.
Food and Chemical Toxicology, 106, 616-623.
https://doi.org/10.1016/j.fct.2016.12.023
Cabarkapa A, Dekanski D, Živković L, Milanovic-Cabarkapa M, Bajić VP, Topalović D, Giampieri F, Gasparrini M, Battino M, Spremo-Potparević B. Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. Food and Chemical Toxicology. 2017;106:616-623
Cabarkapa Andrea, Dekanski Dragana, Živković Lada, Milanovic-Cabarkapa Mirjana, Bajić Vladan P., Topalović Dijana, Giampieri Francesca, Gasparrini Massimiliano, Battino Maurizio, Spremo-Potparević Biljana, "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy" Food and Chemical Toxicology, 106 (2017):616-623,
https://doi.org/10.1016/j.fct.2016.12.023 .
11
7
7

Treatment of Alzheimers Disease: Classical Therapeutic Approach

Bajić, Vladan P.; Sudar, Emina; Spremo-Potparević, Biljana; Živković, Lada; Milićević, Zorka T.; Stanimirović, Julijana; Bogdanović, Nikola; Isenović, Esma R.

(2016)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Sudar, Emina
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Milićević, Zorka T.
AU  - Stanimirović, Julijana
AU  - Bogdanović, Nikola
AU  - Isenović, Esma R.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1039
AB  - Alzheimers disease (AD) is a complex and progressive neurodegenerative disorder, and represents the most common form of dementia. The number of people affected by AD is estimated to be doubled by the year of 2050, and more than 100 million people worldwide will be affected by this disease. Still, there is no reliable diagnostic test which would indicate pre-symptomatic conditions or an increased risk of developing AD. The only drugs approved by the FDA belong to the cholinesterase inhibitors (ChEI) group, such as donepezil, rivastigmine, galantamine and memantine that belongs to a class of drugs named receptor NMDA antagonists. Most mainstream pharmacotherapeutic approaches act by slowing the progression of the condition rather than to treat or prevent the cause of AD. In this review we are presenting literature data from recent research related to new avenues in the classical approach to prevention and treatment of AD.
T2  - Current Pharmaceutical Analysis
T1  - Treatment of Alzheimers Disease: Classical Therapeutic Approach
VL  - 12
IS  - 2
SP  - 82
EP  - 90
DO  - 10.2174/1573412911666150611184740
ER  - 
@article{
author = "Bajić, Vladan P. and Sudar, Emina and Spremo-Potparević, Biljana and Živković, Lada and Milićević, Zorka T. and Stanimirović, Julijana and Bogdanović, Nikola and Isenović, Esma R.",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1039",
abstract = "Alzheimers disease (AD) is a complex and progressive neurodegenerative disorder, and represents the most common form of dementia. The number of people affected by AD is estimated to be doubled by the year of 2050, and more than 100 million people worldwide will be affected by this disease. Still, there is no reliable diagnostic test which would indicate pre-symptomatic conditions or an increased risk of developing AD. The only drugs approved by the FDA belong to the cholinesterase inhibitors (ChEI) group, such as donepezil, rivastigmine, galantamine and memantine that belongs to a class of drugs named receptor NMDA antagonists. Most mainstream pharmacotherapeutic approaches act by slowing the progression of the condition rather than to treat or prevent the cause of AD. In this review we are presenting literature data from recent research related to new avenues in the classical approach to prevention and treatment of AD.",
journal = "Current Pharmaceutical Analysis",
title = "Treatment of Alzheimers Disease: Classical Therapeutic Approach",
volume = "12",
number = "2",
pages = "82-90",
doi = "10.2174/1573412911666150611184740"
}
Bajić, V. P., Sudar, E., Spremo-Potparević, B., Živković, L., Milićević, Z. T., Stanimirović, J., Bogdanović, N.,& Isenović, E. R. (2016). Treatment of Alzheimers Disease: Classical Therapeutic Approach.
Current Pharmaceutical Analysis, 12(2), 82-90.
https://doi.org/10.2174/1573412911666150611184740
Bajić VP, Sudar E, Spremo-Potparević B, Živković L, Milićević ZT, Stanimirović J, Bogdanović N, Isenović ER. Treatment of Alzheimers Disease: Classical Therapeutic Approach. Current Pharmaceutical Analysis. 2016;12(2):82-90
Bajić Vladan P., Sudar Emina, Spremo-Potparević Biljana, Živković Lada, Milićević Zorka T., Stanimirović Julijana, Bogdanović Nikola, Isenović Esma R., "Treatment of Alzheimers Disease: Classical Therapeutic Approach" Current Pharmaceutical Analysis, 12, no. 2 (2016):82-90,
https://doi.org/10.2174/1573412911666150611184740 .
15
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12

Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)

Živković, Lada; Cabarkapa, Andrea; Marcetic, Mirjana; Kovacevic, Nada; Bajić, Vladan P.; Jovicic, Snezana; Spremo-Potparević, Biljana

(2016)

TY  - JOUR
AU  - Živković, Lada
AU  - Cabarkapa, Andrea
AU  - Marcetic, Mirjana
AU  - Kovacevic, Nada
AU  - Bajić, Vladan P.
AU  - Jovicic, Snezana
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1121
AB  - The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.
T2  - Archives of biological sciences
T1  - Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)
VL  - 68
IS  - 1
SP  - 135
EP  - 144
DO  - 10.2298/ABS150512135Z
ER  - 
@article{
author = "Živković, Lada and Cabarkapa, Andrea and Marcetic, Mirjana and Kovacevic, Nada and Bajić, Vladan P. and Jovicic, Snezana and Spremo-Potparević, Biljana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1121",
abstract = "The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.",
journal = "Archives of biological sciences",
title = "Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)",
volume = "68",
number = "1",
pages = "135-144",
doi = "10.2298/ABS150512135Z"
}
Živković, L., Cabarkapa, A., Marcetic, M., Kovacevic, N., Bajić, V. P., Jovicic, S.,& Spremo-Potparević, B. (2016). Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae).
Archives of biological sciences, 68(1), 135-144.
https://doi.org/10.2298/ABS150512135Z
Živković L, Cabarkapa A, Marcetic M, Kovacevic N, Bajić VP, Jovicic S, Spremo-Potparević B. Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae). Archives of biological sciences. 2016;68(1):135-144
Živković Lada, Cabarkapa Andrea, Marcetic Mirjana, Kovacevic Nada, Bajić Vladan P., Jovicic Snezana, Spremo-Potparević Biljana, "Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)" Archives of biological sciences, 68, no. 1 (2016):135-144,
https://doi.org/10.2298/ABS150512135Z .
2
1
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Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study

Cabarkapa, Andrea; Živković, Lada; Borozan, Sunčica Z.; Zlatkovic-Svenda, Mirjana; Dekanski, Dragana; Jancic, Ivan; Radak-Perovic, Marija; Bajić, Vladan P.; Spremo-Potparević, Biljana

(2016)

TY  - JOUR
AU  - Cabarkapa, Andrea
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Zlatkovic-Svenda, Mirjana
AU  - Dekanski, Dragana
AU  - Jancic, Ivan
AU  - Radak-Perovic, Marija
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1282
AB  - The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright (c) 2016 John Wiley and Sons, Ltd.
T2  - Phytotherapy Research
T1  - Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study
VL  - 30
IS  - 10
SP  - 1615
EP  - 1623
DO  - 10.1002/ptr.5662
ER  - 
@article{
author = "Cabarkapa, Andrea and Živković, Lada and Borozan, Sunčica Z. and Zlatkovic-Svenda, Mirjana and Dekanski, Dragana and Jancic, Ivan and Radak-Perovic, Marija and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1282",
abstract = "The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright (c) 2016 John Wiley and Sons, Ltd.",
journal = "Phytotherapy Research",
title = "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study",
volume = "30",
number = "10",
pages = "1615-1623",
doi = "10.1002/ptr.5662"
}
Cabarkapa, A., Živković, L., Borozan, S. Z., Zlatkovic-Svenda, M., Dekanski, D., Jancic, I., Radak-Perovic, M., Bajić, V. P.,& Spremo-Potparević, B. (2016). Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study.
Phytotherapy Research, 30(10), 1615-1623.
https://doi.org/10.1002/ptr.5662
Cabarkapa A, Živković L, Borozan SZ, Zlatkovic-Svenda M, Dekanski D, Jancic I, Radak-Perovic M, Bajić VP, Spremo-Potparević B. Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. Phytotherapy Research. 2016;30(10):1615-1623
Cabarkapa Andrea, Živković Lada, Borozan Sunčica Z., Zlatkovic-Svenda Mirjana, Dekanski Dragana, Jancic Ivan, Radak-Perovic Marija, Bajić Vladan P., Spremo-Potparević Biljana, "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study" Phytotherapy Research, 30, no. 10 (2016):1615-1623,
https://doi.org/10.1002/ptr.5662 .
1
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Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay

Vasiljevic, Jovana; Živković, Lada; Cabarkapa, Andrea; Bajić, Vladan P.; Djelic, Ninoslav; Spremo-Potparević, Biljana

(2016)

TY  - JOUR
AU  - Vasiljevic, Jovana
AU  - Živković, Lada
AU  - Cabarkapa, Andrea
AU  - Bajić, Vladan P.
AU  - Djelic, Ninoslav
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1311
AB  - Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( LT 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction ( GT 95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.
T2  - Alternative Therapies in Health and Medicine
T1  - Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay
VL  - 22
SP  - 24
EP  - 31
ER  - 
@article{
author = "Vasiljevic, Jovana and Živković, Lada and Cabarkapa, Andrea and Bajić, Vladan P. and Djelic, Ninoslav and Spremo-Potparević, Biljana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1311",
abstract = "Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( LT 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction ( GT 95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.",
journal = "Alternative Therapies in Health and Medicine",
title = "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay",
volume = "22",
pages = "24-31"
}
Vasiljevic, J., Živković, L., Cabarkapa, A., Bajić, V. P., Djelic, N.,& Spremo-Potparević, B. (2016). Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay.
Alternative Therapies in Health and Medicine, 22, 24-31.
Vasiljevic J, Živković L, Cabarkapa A, Bajić VP, Djelic N, Spremo-Potparević B. Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay. Alternative Therapies in Health and Medicine. 2016;22:24-31
Vasiljevic Jovana, Živković Lada, Cabarkapa Andrea, Bajić Vladan P., Djelic Ninoslav, Spremo-Potparević Biljana, "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay" Alternative Therapies in Health and Medicine, 22 (2016):24-31
5

Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps

Cilerdzic, Jasmina; Stajic, Mirjana; Živković, Lada; Vukojevic, Jelena; Bajić, Vladan P.; Spremo-Potparević, Biljana

(2016)

TY  - JOUR
AU  - Cilerdzic, Jasmina
AU  - Stajic, Mirjana
AU  - Živković, Lada
AU  - Vukojevic, Jelena
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1448
AB  - Ganoderma lucidum is traditionally used in Eastern medicine to preserve vitality, promote longevity, and treat disease. It possesses immunomodulatory, antitumor, antimicrobial, and antiaging activities, among others, but one of the most important is its antioxidant property, which is the basis for other effects, because free radicals trigger many diseases. The substrate commonly used for commercial cultivation of G. lucidum is not environmenwas to analyze the effect of substrate composition on the bioactivity of G. lucidum basidiocarps. G. lucidum was cultivated on 2 different substrates: (1) a mixture of wheat straw, grapevine branches, and wheat bran, and (2) wheat straw. Commercial fruiting bodies, cultivated on oak sawdust, were used as the control. 1,1- diphenyl- 2- picrylhydrazyl (DPPH) The comet test was performed to detect the degree of DNA damage in the cells that were exposed to G. lucidum extracts before and after the effect of oxidants. Higher antioxidative potential was observed for the extract of G. lucidum basidiocarps cultivated on wheat straw compared with that from the mixed substrate and especially with commercial ones. The alternatively cultivated basidiocarps also showed stronger antigenotoxic potential compared with commercial ones. The study showed that fruiting bodies produced on wheat straw, one of the most accessible and cheapest crop residues, are more potent antioxidant and antigenotoxic agents than commercially cultivated ones.
T2  - International Journal of Medicinal Mushrooms
T1  - Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps
VL  - 18
IS  - 12
SP  - 1061
EP  - 1069
DO  - 10.1615/IntJMedMushrooms.v18.i12.10
ER  - 
@article{
author = "Cilerdzic, Jasmina and Stajic, Mirjana and Živković, Lada and Vukojevic, Jelena and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1448",
abstract = "Ganoderma lucidum is traditionally used in Eastern medicine to preserve vitality, promote longevity, and treat disease. It possesses immunomodulatory, antitumor, antimicrobial, and antiaging activities, among others, but one of the most important is its antioxidant property, which is the basis for other effects, because free radicals trigger many diseases. The substrate commonly used for commercial cultivation of G. lucidum is not environmenwas to analyze the effect of substrate composition on the bioactivity of G. lucidum basidiocarps. G. lucidum was cultivated on 2 different substrates: (1) a mixture of wheat straw, grapevine branches, and wheat bran, and (2) wheat straw. Commercial fruiting bodies, cultivated on oak sawdust, were used as the control. 1,1- diphenyl- 2- picrylhydrazyl (DPPH) The comet test was performed to detect the degree of DNA damage in the cells that were exposed to G. lucidum extracts before and after the effect of oxidants. Higher antioxidative potential was observed for the extract of G. lucidum basidiocarps cultivated on wheat straw compared with that from the mixed substrate and especially with commercial ones. The alternatively cultivated basidiocarps also showed stronger antigenotoxic potential compared with commercial ones. The study showed that fruiting bodies produced on wheat straw, one of the most accessible and cheapest crop residues, are more potent antioxidant and antigenotoxic agents than commercially cultivated ones.",
journal = "International Journal of Medicinal Mushrooms",
title = "Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps",
volume = "18",
number = "12",
pages = "1061-1069",
doi = "10.1615/IntJMedMushrooms.v18.i12.10"
}
Cilerdzic, J., Stajic, M., Živković, L., Vukojevic, J., Bajić, V. P.,& Spremo-Potparević, B. (2016). Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps.
International Journal of Medicinal Mushrooms, 18(12), 1061-1069.
https://doi.org/10.1615/IntJMedMushrooms.v18.i12.10
Cilerdzic J, Stajic M, Živković L, Vukojevic J, Bajić VP, Spremo-Potparević B. Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps. International Journal of Medicinal Mushrooms. 2016;18(12):1061-1069
Cilerdzic Jasmina, Stajic Mirjana, Živković Lada, Vukojevic Jelena, Bajić Vladan P., Spremo-Potparević Biljana, "Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps" International Journal of Medicinal Mushrooms, 18, no. 12 (2016):1061-1069,
https://doi.org/10.1615/IntJMedMushrooms.v18.i12.10 .
1
3
3
4

Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review

Bajić, Vladan P.; Spremo-Potparević, Biljana; Živković, Lada; Sudar, Emina; Zafirović, Sonja; Obradović, Milan M.; Isenović, Esma R.

(2015)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Sudar, Emina
AU  - Zafirović, Sonja
AU  - Obradović, Milan M.
AU  - Isenović, Esma R.
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/398
AB  - Alzheimers disease (AD) is a multi factorial disease, related to the loss of neurons and synapses in cerebral cortex and subcortical structures, leading to degenerative changes and atrophy. Despite abundance of facts related to AD and its pathology, the only drugs used in the prevention and treatment are those from the cholinesterase inhibitors group. However, there is growing evidence that a non-classical therapeutic approach in the treatment of AD has beneficial effects. In this review we summarized recent literature data related to the non-classical drugs for the treatment of AD predominantly used in clinical testing, such as amyloid aggregation inhibitors, beta-sheet breakers, antioxidants, estrogens and immunotherapeutics.
T2  - Letters in Drug Design and Discovery
T1  - Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review
VL  - 12
IS  - 2
SP  - 158
EP  - 164
ER  - 
@article{
author = "Bajić, Vladan P. and Spremo-Potparević, Biljana and Živković, Lada and Sudar, Emina and Zafirović, Sonja and Obradović, Milan M. and Isenović, Esma R.",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/398",
abstract = "Alzheimers disease (AD) is a multi factorial disease, related to the loss of neurons and synapses in cerebral cortex and subcortical structures, leading to degenerative changes and atrophy. Despite abundance of facts related to AD and its pathology, the only drugs used in the prevention and treatment are those from the cholinesterase inhibitors group. However, there is growing evidence that a non-classical therapeutic approach in the treatment of AD has beneficial effects. In this review we summarized recent literature data related to the non-classical drugs for the treatment of AD predominantly used in clinical testing, such as amyloid aggregation inhibitors, beta-sheet breakers, antioxidants, estrogens and immunotherapeutics.",
journal = "Letters in Drug Design and Discovery",
title = "Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review",
volume = "12",
number = "2",
pages = "158-164"
}
Bajić, V. P., Spremo-Potparević, B., Živković, L., Sudar, E., Zafirović, S., Obradović, M. M.,& Isenović, E. R. (2015). Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review.
Letters in Drug Design and Discovery, 12(2), 158-164.
Bajić VP, Spremo-Potparević B, Živković L, Sudar E, Zafirović S, Obradović MM, Isenović ER. Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review. Letters in Drug Design and Discovery. 2015;12(2):158-164
Bajić Vladan P., Spremo-Potparević Biljana, Živković Lada, Sudar Emina, Zafirović Sonja, Obradović Milan M., Isenović Esma R., "Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review" Letters in Drug Design and Discovery, 12, no. 2 (2015):158-164
3

Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease

Bajić, Vladan P.; Mandušić, Vesna; Stefanova, Elka; Božović, Ana M.; Davidović, Radoslav S.; Živković, Lada; Cabarkapa, Andrea; Spremo-Potparević, Biljana

(2015)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Mandušić, Vesna
AU  - Stefanova, Elka
AU  - Božović, Ana M.
AU  - Davidović, Radoslav S.
AU  - Živković, Lada
AU  - Cabarkapa, Andrea
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/314
AB  - X-chromosome instability has been a long established feature in Alzheimers disease ( AD). Premature centromere division and aneuploidy of the X-chromosome has been found in peripheral blood lymphocytes and neuronal tissue in female AD patients. Interestingly, only one chromosome of the X pair has been affected. These results raised a question, Is the X-chromosome inactivation pattern altered in peripheral blood lymphocytes ofwomen affected by AD? To address this question, we analyzed the methylation status of androgen receptor promoter which may show us any deviation from the 50 : 50% X inactivation status in peripheral blood lymphocytes ofwomen with AD. Our results showed skewed inactivation patterns ( GT 90%). These findings suggest that an epigenetic alteration on the inactivation centers of the X-chromosome (or skewing) relates not only to aging, by might be a novel property that could account for the higher incidence of AD in women.
T2  - Journal of Alzheimers Disease
T1  - Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease
VL  - 43
IS  - 4
SP  - 1251
EP  - 1259
DO  - 10.3233/JAD-141674
ER  - 
@article{
author = "Bajić, Vladan P. and Mandušić, Vesna and Stefanova, Elka and Božović, Ana M. and Davidović, Radoslav S. and Živković, Lada and Cabarkapa, Andrea and Spremo-Potparević, Biljana",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/314",
abstract = "X-chromosome instability has been a long established feature in Alzheimers disease ( AD). Premature centromere division and aneuploidy of the X-chromosome has been found in peripheral blood lymphocytes and neuronal tissue in female AD patients. Interestingly, only one chromosome of the X pair has been affected. These results raised a question, Is the X-chromosome inactivation pattern altered in peripheral blood lymphocytes ofwomen affected by AD? To address this question, we analyzed the methylation status of androgen receptor promoter which may show us any deviation from the 50 : 50% X inactivation status in peripheral blood lymphocytes ofwomen with AD. Our results showed skewed inactivation patterns ( GT 90%). These findings suggest that an epigenetic alteration on the inactivation centers of the X-chromosome (or skewing) relates not only to aging, by might be a novel property that could account for the higher incidence of AD in women.",
journal = "Journal of Alzheimers Disease",
title = "Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease",
volume = "43",
number = "4",
pages = "1251-1259",
doi = "10.3233/JAD-141674"
}
Bajić, V. P., Mandušić, V., Stefanova, E., Božović, A. M., Davidović, R. S., Živković, L., Cabarkapa, A.,& Spremo-Potparević, B. (2015). Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease.
Journal of Alzheimers Disease, 43(4), 1251-1259.
https://doi.org/10.3233/JAD-141674
Bajić VP, Mandušić V, Stefanova E, Božović AM, Davidović RS, Živković L, Cabarkapa A, Spremo-Potparević B. Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease. Journal of Alzheimers Disease. 2015;43(4):1251-1259
Bajić Vladan P., Mandušić Vesna, Stefanova Elka, Božović Ana M., Davidović Radoslav S., Živković Lada, Cabarkapa Andrea, Spremo-Potparević Biljana, "Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease" Journal of Alzheimers Disease, 43, no. 4 (2015):1251-1259,
https://doi.org/10.3233/JAD-141674 .
1
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11

Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro

Topalović Žukovec, Dijana; Živković, Lada; Cabarkapa, Andrea; Djelic, Ninoslav; Bajić, Vladan P.; Dekanski, Dragana; Spremo-Potparević, Biljana

(2015)

TY  - JOUR
AU  - Topalović Žukovec, Dijana
AU  - Živković, Lada
AU  - Cabarkapa, Andrea
AU  - Djelic, Ninoslav
AU  - Bajić, Vladan P.
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/432
AB  - The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P LT 0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro
DO  - 10.1155/2015/762192
ER  - 
@article{
author = "Topalović Žukovec, Dijana and Živković, Lada and Cabarkapa, Andrea and Djelic, Ninoslav and Bajić, Vladan P. and Dekanski, Dragana and Spremo-Potparević, Biljana",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/432",
abstract = "The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P LT 0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro",
doi = "10.1155/2015/762192"
}
Topalović Žukovec, D., Živković, L., Cabarkapa, A., Djelic, N., Bajić, V. P., Dekanski, D.,& Spremo-Potparević, B. (2015). Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro.
Oxidative Medicine and Cellular Longevity.
https://doi.org/10.1155/2015/762192
Topalović Žukovec D, Živković L, Cabarkapa A, Djelic N, Bajić VP, Dekanski D, Spremo-Potparević B. Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. Oxidative Medicine and Cellular Longevity. 2015;
Topalović Žukovec Dijana, Živković Lada, Cabarkapa Andrea, Djelic Ninoslav, Bajić Vladan P., Dekanski Dragana, Spremo-Potparević Biljana, "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro" Oxidative Medicine and Cellular Longevity (2015),
https://doi.org/10.1155/2015/762192 .
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Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability

Bajić, Vladan P.; Spremo-Potparević, Biljana; Živković, Lada; Isenović, Esma R.; Arendt, Thomas

(2015)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Isenović, Esma R.
AU  - Arendt, Thomas
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/664
AB  - Neurons are postmitotic cells that are in permanent cell cycle arrest. However, components of the cell cycle machinery that are expressed in Alzheimers disease (AD) neurons are showing features of a cycling cell and those attributed to a postmitotic cell as well. Furthermore, the unique physiological operations taking place in neurons, ascribed to core cell cycle regulators are also key regulators in cell division. Functions of these cell cycle regulators include neuronal migration, axonal elongation, axon pruning, dendrite morphogenesis and synaptic maturation and plasticity. In this review, we focus on cohesion and cohesion related proteins in reference to their neuronal functions and how impaired centromere/cohesion dynamics may connect cell cycle dysfunction to aneuploidy in AD. (C) 2015 Elsevier Ltd. All rights reserved.
T2  - Neuroscience and Biobehavioral Reviews
T1  - Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability
VL  - 55
SP  - 365
EP  - 374
DO  - 10.1016/j.neubiorev.2015.05.010
ER  - 
@article{
author = "Bajić, Vladan P. and Spremo-Potparević, Biljana and Živković, Lada and Isenović, Esma R. and Arendt, Thomas",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/664",
abstract = "Neurons are postmitotic cells that are in permanent cell cycle arrest. However, components of the cell cycle machinery that are expressed in Alzheimers disease (AD) neurons are showing features of a cycling cell and those attributed to a postmitotic cell as well. Furthermore, the unique physiological operations taking place in neurons, ascribed to core cell cycle regulators are also key regulators in cell division. Functions of these cell cycle regulators include neuronal migration, axonal elongation, axon pruning, dendrite morphogenesis and synaptic maturation and plasticity. In this review, we focus on cohesion and cohesion related proteins in reference to their neuronal functions and how impaired centromere/cohesion dynamics may connect cell cycle dysfunction to aneuploidy in AD. (C) 2015 Elsevier Ltd. All rights reserved.",
journal = "Neuroscience and Biobehavioral Reviews",
title = "Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability",
volume = "55",
pages = "365-374",
doi = "10.1016/j.neubiorev.2015.05.010"
}
Bajić, V. P., Spremo-Potparević, B., Živković, L., Isenović, E. R.,& Arendt, T. (2015). Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability.
Neuroscience and Biobehavioral Reviews, 55, 365-374.
https://doi.org/10.1016/j.neubiorev.2015.05.010
Bajić VP, Spremo-Potparević B, Živković L, Isenović ER, Arendt T. Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability. Neuroscience and Biobehavioral Reviews. 2015;55:365-374
Bajić Vladan P., Spremo-Potparević Biljana, Živković Lada, Isenović Esma R., Arendt Thomas, "Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability" Neuroscience and Biobehavioral Reviews, 55 (2015):365-374,
https://doi.org/10.1016/j.neubiorev.2015.05.010 .
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21

Implications of oxidative stress in occupational exposure to lead on a cellular level

Cabarkapa, Andrea; Borozan, Sunčica Z.; Živković, Lada; Milanovic-Cabarkapa, Mirjana; Stojanovic, Srdan; Bajić, Vladan P.; Spremo-Potparević, Biljana

(2015)

TY  - JOUR
AU  - Cabarkapa, Andrea
AU  - Borozan, Sunčica Z.
AU  - Živković, Lada
AU  - Milanovic-Cabarkapa, Mirjana
AU  - Stojanovic, Srdan
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/703
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
T2  - Toxicological and Environmental Chemistry
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 
@article{
author = "Cabarkapa, Andrea and Borozan, Sunčica Z. and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Stojanovic, Srdan and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/703",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
journal = "Toxicological and Environmental Chemistry",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}
Cabarkapa, A., Borozan, S. Z., Živković, L., Milanovic-Cabarkapa, M., Stojanovic, S., Bajić, V. P.,& Spremo-Potparević, B. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level.
Toxicological and Environmental Chemistry, 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973
Cabarkapa A, Borozan SZ, Živković L, Milanovic-Cabarkapa M, Stojanovic S, Bajić VP, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. Toxicological and Environmental Chemistry. 2015;97(6):799-813
Cabarkapa Andrea, Borozan Sunčica Z., Živković Lada, Milanovic-Cabarkapa Mirjana, Stojanovic Srdan, Bajić Vladan P., Spremo-Potparević Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" Toxicological and Environmental Chemistry, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 .
5
3
4

Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients

Spremo-Potparević, Biljana; Bajić, Vladan P.; Perry, George; Živković, Lada

(2015)

TY  - JOUR
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan P.
AU  - Perry, George
AU  - Živković, Lada
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/844
AB  - Chromosomal alterations as a sign of genetic instability are a feature of Alzheimers disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p LT 0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p LT 0.001). In addition, a strong (R2=0.97, n=20) and significant (p LT 0.001) correlation was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.
T2  - Current Alzheimer Research
T1  - Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients
VL  - 12
IS  - 10
SP  - 990
EP  - 996
DO  - 10.2174/1567205012666151027124154
ER  - 
@article{
author = "Spremo-Potparević, Biljana and Bajić, Vladan P. and Perry, George and Živković, Lada",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/844",
abstract = "Chromosomal alterations as a sign of genetic instability are a feature of Alzheimers disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p LT 0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p LT 0.001). In addition, a strong (R2=0.97, n=20) and significant (p LT 0.001) correlation was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.",
journal = "Current Alzheimer Research",
title = "Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients",
volume = "12",
number = "10",
pages = "990-996",
doi = "10.2174/1567205012666151027124154"
}
Spremo-Potparević, B., Bajić, V. P., Perry, G.,& Živković, L. (2015). Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients.
Current Alzheimer Research, 12(10), 990-996.
https://doi.org/10.2174/1567205012666151027124154
Spremo-Potparević B, Bajić VP, Perry G, Živković L. Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients. Current Alzheimer Research. 2015;12(10):990-996
Spremo-Potparević Biljana, Bajić Vladan P., Perry George, Živković Lada, "Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients" Current Alzheimer Research, 12, no. 10 (2015):990-996,
https://doi.org/10.2174/1567205012666151027124154 .
5
4
3

CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study

Cabarkapa, A.; Borozan, Sunčica Z.; Zivkovic, L.; Stojanovic, S.; Milanovic-Cabarkapa, M.; Bajić, Vladan P.; Spremo-Potparević, Biljana

(2015)

TY  - JOUR
AU  - Cabarkapa, A.
AU  - Borozan, Sunčica Z.
AU  - Zivkovic, L.
AU  - Stojanovic, S.
AU  - Milanovic-Cabarkapa, M.
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/873
AB  - Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Chemico-biological Interactions
T1  - CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study
VL  - 242
SP  - 171
EP  - 178
DO  - 10.1016/j.cbi.2015.10.002
ER  - 
@article{
author = "Cabarkapa, A. and Borozan, Sunčica Z. and Zivkovic, L. and Stojanovic, S. and Milanovic-Cabarkapa, M. and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/873",
abstract = "Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Chemico-biological Interactions",
title = "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study",
volume = "242",
pages = "171-178",
doi = "10.1016/j.cbi.2015.10.002"
}
Cabarkapa, A., Borozan, S. Z., Zivkovic, L., Stojanovic, S., Milanovic-Cabarkapa, M., Bajić, V. P.,& Spremo-Potparević, B. (2015). CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study.
Chemico-biological Interactions, 242, 171-178.
https://doi.org/10.1016/j.cbi.2015.10.002
Cabarkapa A, Borozan SZ, Zivkovic L, Stojanovic S, Milanovic-Cabarkapa M, Bajić VP, Spremo-Potparević B. CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. Chemico-biological Interactions. 2015;242:171-178
Cabarkapa A., Borozan Sunčica Z., Zivkovic L., Stojanovic S., Milanovic-Cabarkapa M., Bajić Vladan P., Spremo-Potparević Biljana, "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study" Chemico-biological Interactions, 242 (2015):171-178,
https://doi.org/10.1016/j.cbi.2015.10.002 .
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Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer

Milićević, Zorka T.; Kasapović, Jelena; Gavrilović, Ljubica; Milovanović, Zorka M.; Bajić, Vladan P.; Spremo-Potparević, Biljana

(2014)

TY  - JOUR
AU  - Milićević, Zorka T.
AU  - Kasapović, Jelena
AU  - Gavrilović, Ljubica
AU  - Milovanović, Zorka M.
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/113
AB  - It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p LT 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p LT 0.001; catalase, CAT, p LT 0.01; glutathione reductase, GR, p LT 0.001; glutathione, GSH, p LT 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p LT 0.05) and lowered antioxidant activity of CAT (- 0.437, p LT 0.01) in the breast cancer patients.
T2  - EXCLI Journal
T1  - Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer
VL  - 13
SP  - 691
EP  - 708
ER  - 
@article{
author = "Milićević, Zorka T. and Kasapović, Jelena and Gavrilović, Ljubica and Milovanović, Zorka M. and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/113",
abstract = "It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p LT 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p LT 0.001; catalase, CAT, p LT 0.01; glutathione reductase, GR, p LT 0.001; glutathione, GSH, p LT 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p LT 0.05) and lowered antioxidant activity of CAT (- 0.437, p LT 0.01) in the breast cancer patients.",
journal = "EXCLI Journal",
title = "Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer",
volume = "13",
pages = "691-708"
}
Milićević, Z. T., Kasapović, J., Gavrilović, L., Milovanović, Z. M., Bajić, V. P.,& Spremo-Potparević, B. (2014). Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer.
EXCLI Journal, 13, 691-708.
Milićević ZT, Kasapović J, Gavrilović L, Milovanović ZM, Bajić VP, Spremo-Potparević B. Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer. EXCLI Journal. 2014;13:691-708
Milićević Zorka T., Kasapović Jelena, Gavrilović Ljubica, Milovanović Zorka M., Bajić Vladan P., Spremo-Potparević Biljana, "Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer" EXCLI Journal, 13 (2014):691-708
5

Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells

Milićević, Zorka T.; Bajić, Vladan P.; Živković, Lada; Kasapović, Jelena; Anđelković, Uros; Spremo-Potparević, Biljana

(2014)

TY  - JOUR
AU  - Milićević, Zorka T.
AU  - Bajić, Vladan P.
AU  - Živković, Lada
AU  - Kasapović, Jelena
AU  - Anđelković, Uros
AU  - Spremo-Potparević, Biljana
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5855
AB  - In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.
T2  - Scientific World Journal
T1  - Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells
DO  - 10.1155/2014/618698
ER  - 
@article{
author = "Milićević, Zorka T. and Bajić, Vladan P. and Živković, Lada and Kasapović, Jelena and Anđelković, Uros and Spremo-Potparević, Biljana",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5855",
abstract = "In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.",
journal = "Scientific World Journal",
title = "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells",
doi = "10.1155/2014/618698"
}
Milićević, Z. T., Bajić, V. P., Živković, L., Kasapović, J., Anđelković, U.,& Spremo-Potparević, B. (2014). Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells.
Scientific World Journal.
https://doi.org/10.1155/2014/618698
Milićević ZT, Bajić VP, Živković L, Kasapović J, Anđelković U, Spremo-Potparević B. Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells. Scientific World Journal. 2014;
Milićević Zorka T., Bajić Vladan P., Živković Lada, Kasapović Jelena, Anđelković Uros, Spremo-Potparević Biljana, "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells" Scientific World Journal (2014),
https://doi.org/10.1155/2014/618698 .
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13

DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division

Živković, Lada; Spremo-Potparević, Biljana; Siedlak, Sandra L.; Perry, George; Plećaš-Solarović, Bosiljka; Milicevic, Zorana; Bajić, Vladan P.

(2013)

TY  - JOUR
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Siedlak, Sandra L.
AU  - Perry, George
AU  - Plećaš-Solarović, Bosiljka
AU  - Milicevic, Zorana
AU  - Bajić, Vladan P.
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5681
AB  - While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p LT 0.01 and 38%, p LT 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p LT 0.01) as well as a higher frequency of DNA damage (37%, p LT 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p LT 0.01 and 2.8 times, p LT 0.01, respectively) and DNA damage (63%, p LT 0.01 and 50%, p LT 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p LT 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright (C) 2013 S. Karger AG, Basel
T2  - Neurodegenerative diseases
T1  - DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division
VL  - 12
IS  - 3
SP  - 156
EP  - 163
DO  - 10.1159/000346114
ER  - 
@article{
author = "Živković, Lada and Spremo-Potparević, Biljana and Siedlak, Sandra L. and Perry, George and Plećaš-Solarović, Bosiljka and Milicevic, Zorana and Bajić, Vladan P.",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5681",
abstract = "While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p LT 0.01 and 38%, p LT 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p LT 0.01) as well as a higher frequency of DNA damage (37%, p LT 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p LT 0.01 and 2.8 times, p LT 0.01, respectively) and DNA damage (63%, p LT 0.01 and 50%, p LT 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p LT 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright (C) 2013 S. Karger AG, Basel",
journal = "Neurodegenerative diseases",
title = "DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division",
volume = "12",
number = "3",
pages = "156-163",
doi = "10.1159/000346114"
}
Živković, L., Spremo-Potparević, B., Siedlak, S. L., Perry, G., Plećaš-Solarović, B., Milicevic, Z.,& Bajić, V. P. (2013). DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division.
Neurodegenerative diseases, 12(3), 156-163.
https://doi.org/10.1159/000346114
Živković L, Spremo-Potparević B, Siedlak SL, Perry G, Plećaš-Solarović B, Milicevic Z, Bajić VP. DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division. Neurodegenerative diseases. 2013;12(3):156-163
Živković Lada, Spremo-Potparević Biljana, Siedlak Sandra L., Perry George, Plećaš-Solarović Bosiljka, Milicevic Zorana, Bajić Vladan P., "DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division" Neurodegenerative diseases, 12, no. 3 (2013):156-163,
https://doi.org/10.1159/000346114 .
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