TY  - JOUR
AU  - Kasapović, Jelena
AU  - Pejić, Snežana
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Radosevic-Jelic, Ljiljana
AU  - Saičić, Zorica S.
AU  - Pajović, Snežana B.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4107
AB  - Objectives: Breast carcinoma is related to the increase of lipid peroxidation in plasma with concomitant decrease of antioxidant (AO) defense capacity in blood cells, which becomes more pronounced during aging of the patients. This work evaluated the potential age-related effect of chemotherapy with 5-fluorouracil, doxorubicin and cyclophosphamide (FAC) on the level of lipid hydroperoxides (LP), glutathione (GSH), AO enzyme activities of copper, zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in breast cancer patients. The level of CuZnSOD protein was assessed after the FAC therapy and radiotherapy of breast cancer. Design and methods: AO parameters were measured in the blood of 58 breast cancer patients and 60 healthy age-matched healthy subjects by biochemical and Western blot analyses. Results: Increased oxidative stress (LP: p LT 0.05) and decreased AO enzyme activities (CuZnSOD: p LT 0.01, GPx: p LT 0.05, GR: p LT 0.01) and GSH level (p LT 0.01) in the blood of breast cancer patients in response to FAC chemotherapy seem not to be age-dependent. CuZnSOD enzyme expression decreased after the FAC chemotherapy (p LT 0.05), while it increased after the radiotherapy of breast cancer (p LT 0.05). Conclusion: FAC chemotherapy and radiotherapy promote further oxidative shift, which potentiate already existing chronic oxidative stress linked to breast cancer. In these effects, impaired capacity for H2O2 detoxification (CAT. GPX and GSH) seems to have major contribution. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
T2  - Clinical Biochemistry
T1  - Antioxidant status and lipid peroxidation in the blood of breast cancer patients of different ages after chemotherapy with 5-fluorouracil, doxorubicin and cyclophosphamide
VL  - 43
IS  - 16-17
SP  - 1287
EP  - 1293
DO  - 10.1016/j.clinbiochem.2010.08.009
ER  - 

@article{
author = "Kasapović, Jelena and Pejić, Snežana and Stojiljković, Vesna and Todorović, Ana and Radosevic-Jelic, Ljiljana and Saičić, Zorica S. and Pajović, Snežana B.",
year = "2010",
abstract = "Objectives: Breast carcinoma is related to the increase of lipid peroxidation in plasma with concomitant decrease of antioxidant (AO) defense capacity in blood cells, which becomes more pronounced during aging of the patients. This work evaluated the potential age-related effect of chemotherapy with 5-fluorouracil, doxorubicin and cyclophosphamide (FAC) on the level of lipid hydroperoxides (LP), glutathione (GSH), AO enzyme activities of copper, zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in breast cancer patients. The level of CuZnSOD protein was assessed after the FAC therapy and radiotherapy of breast cancer. Design and methods: AO parameters were measured in the blood of 58 breast cancer patients and 60 healthy age-matched healthy subjects by biochemical and Western blot analyses. Results: Increased oxidative stress (LP: p LT 0.05) and decreased AO enzyme activities (CuZnSOD: p LT 0.01, GPx: p LT 0.05, GR: p LT 0.01) and GSH level (p LT 0.01) in the blood of breast cancer patients in response to FAC chemotherapy seem not to be age-dependent. CuZnSOD enzyme expression decreased after the FAC chemotherapy (p LT 0.05), while it increased after the radiotherapy of breast cancer (p LT 0.05). Conclusion: FAC chemotherapy and radiotherapy promote further oxidative shift, which potentiate already existing chronic oxidative stress linked to breast cancer. In these effects, impaired capacity for H2O2 detoxification (CAT. GPX and GSH) seems to have major contribution. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.",
journal = "Clinical Biochemistry",
title = "Antioxidant status and lipid peroxidation in the blood of breast cancer patients of different ages after chemotherapy with 5-fluorouracil, doxorubicin and cyclophosphamide",
volume = "43",
number = "16-17",
pages = "1287-1293",
doi = "10.1016/j.clinbiochem.2010.08.009"
}

Kasapović, J., Pejić, S., Stojiljković, V., Todorović, A., Radosevic-Jelic, L., Saičić, Z. S.,& Pajović, S. B.. (2010). Antioxidant status and lipid peroxidation in the blood of breast cancer patients of different ages after chemotherapy with 5-fluorouracil, doxorubicin and cyclophosphamide. in Clinical Biochemistry, 43(16-17), 1287-1293.
https://doi.org/10.1016/j.clinbiochem.2010.08.009

Kasapović J, Pejić S, Stojiljković V, Todorović A, Radosevic-Jelic L, Saičić ZS, Pajović SB. Antioxidant status and lipid peroxidation in the blood of breast cancer patients of different ages after chemotherapy with 5-fluorouracil, doxorubicin and cyclophosphamide. in Clinical Biochemistry. 2010;43(16-17):1287-1293.
doi:10.1016/j.clinbiochem.2010.08.009 .

Kasapović, Jelena, Pejić, Snežana, Stojiljković, Vesna, Todorović, Ana, Radosevic-Jelic, Ljiljana, Saičić, Zorica S., Pajović, Snežana B., "Antioxidant status and lipid peroxidation in the blood of breast cancer patients of different ages after chemotherapy with 5-fluorouracil, doxorubicin and cyclophosphamide" in Clinical Biochemistry, 43, no. 16-17 (2010):1287-1293,
https://doi.org/10.1016/j.clinbiochem.2010.08.009 . .

TY  - JOUR
AU  - Kasapović, Jelena
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Radosevic-Jelic, Ljiljana
AU  - Pajović, Snežana B.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3689
AB  - In this study we investigated the effects of breast cancer radiotherapy on the antioxidant (AO) enzyme activities of copper, zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), as well as on the concentration of reduced glutathione (GSH) and lipid peroxides (LP) in blood of patients aged 45-58 years and older than 60 years. The results show that in blood of patients aged 45-58 years, radiotherapy increased the activities of CuZnSOD, CAT, and GR, as well as the concentration of GSH, without affecting the activity of GPx and concentration of LP. In patients older than 60 years, radiotherapy increased the activities of CuZnSOD and CAT, lowered the activity of GPx and concentration of GSH, and increased the concentration of LP. Our results indicate that the response to radiotherapy involves age-related impairment of AO capacity for elimination of H2O2, causing oxidative damage to blood cells. This suggests that cytotoxic effects of radiation on healthy tissues might be more pronounced during the aging of breast cancer patients, and should be considered in the further development of individualization protocols in cancer radiotherapy.
T2  - Archives of Biological Sciences
T1  - Antioxidant Status in Breast Cancer Patients of Different Ages After Radiotherapy
VL  - 61
IS  - 1
SP  - 23
EP  - 28
DO  - 10.2298/ABS0901023K
ER  - 

@article{
author = "Kasapović, Jelena and Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Radosevic-Jelic, Ljiljana and Pajović, Snežana B.",
year = "2009",
abstract = "In this study we investigated the effects of breast cancer radiotherapy on the antioxidant (AO) enzyme activities of copper, zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), as well as on the concentration of reduced glutathione (GSH) and lipid peroxides (LP) in blood of patients aged 45-58 years and older than 60 years. The results show that in blood of patients aged 45-58 years, radiotherapy increased the activities of CuZnSOD, CAT, and GR, as well as the concentration of GSH, without affecting the activity of GPx and concentration of LP. In patients older than 60 years, radiotherapy increased the activities of CuZnSOD and CAT, lowered the activity of GPx and concentration of GSH, and increased the concentration of LP. Our results indicate that the response to radiotherapy involves age-related impairment of AO capacity for elimination of H2O2, causing oxidative damage to blood cells. This suggests that cytotoxic effects of radiation on healthy tissues might be more pronounced during the aging of breast cancer patients, and should be considered in the further development of individualization protocols in cancer radiotherapy.",
journal = "Archives of Biological Sciences",
title = "Antioxidant Status in Breast Cancer Patients of Different Ages After Radiotherapy",
volume = "61",
number = "1",
pages = "23-28",
doi = "10.2298/ABS0901023K"
}

Kasapović, J., Pejić, S., Todorović, A., Stojiljković, V., Radosevic-Jelic, L.,& Pajović, S. B.. (2009). Antioxidant Status in Breast Cancer Patients of Different Ages After Radiotherapy. in Archives of Biological Sciences, 61(1), 23-28.
https://doi.org/10.2298/ABS0901023K

Kasapović J, Pejić S, Todorović A, Stojiljković V, Radosevic-Jelic L, Pajović SB. Antioxidant Status in Breast Cancer Patients of Different Ages After Radiotherapy. in Archives of Biological Sciences. 2009;61(1):23-28.
doi:10.2298/ABS0901023K .

Kasapović, Jelena, Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Radosevic-Jelic, Ljiljana, Pajović, Snežana B., "Antioxidant Status in Breast Cancer Patients of Different Ages After Radiotherapy" in Archives of Biological Sciences, 61, no. 1 (2009):23-28,
https://doi.org/10.2298/ABS0901023K . .