TY  - JOUR
AU  - Đurašević, Siniša
AU  - Ružičić, Aleksandra
AU  - Lakić, Iva
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Glumac, Sofija
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Drakulić, Dunja R.
AU  - Stanković, Sanja
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10173
AB  - A dysregulated and overwhelming response to an infection accompanied by the exaggerated pro-inflammatory state and metabolism disturbance leads to the fatal outcome in sepsis. Previously we showed that meldonium, an anti-ischemic drug clinically used to treat myocardial and cerebral ischemia, strongly increases mortality in faecal-induced peritonitis (FIP) in rats. We postulated that the same mechanism that is responsible for the otherwise strong anti-inflammatory effects of meldonium could be the culprit of the increased mortality. In the present study, we applied the LPS-induced model of sepsis to explore the presence of any differences from and/or similarities to the FIP model. When it comes to energy production, despite some shared similarities, it is evident that LPS and FIP models of sepsis differ greatly. A different profile of sympathoadrenal activation may account for this observation, as it was lacking in the FIP model, whereas in the LPS model it was strong enough to overcome the effects of meldonium. Therefore, choosing the appropriate model of sepsis induction is of great importance, especially if energy homeostasis is the main focus of the study. Even when differences in the experimental design of the two models are acknowledged, the role of different patterns of energy production cannot be excluded. On that account, our results draw attention to the importance of uninterrupted energy production in sepsis but also call for much-needed revisions of the current recommendations for its treatment.
T2  - International Journal of Molecular Sciences
T1  - The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats
VL  - 23
IS  - 4
SP  - 2395
DO  - 10.3390/ijms23042395
ER  - 

@article{
author = "Đurašević, Siniša and Ružičić, Aleksandra and Lakić, Iva and Tosti, Tomislav and Đurović, Saša and Glumac, Sofija and Pejić, Snežana and Todorović, Ana and Drakulić, Dunja R. and Stanković, Sanja and Jasnić, Nebojša and Đorđević, Jelena and Todorović, Zoran",
year = "2022",
abstract = "A dysregulated and overwhelming response to an infection accompanied by the exaggerated pro-inflammatory state and metabolism disturbance leads to the fatal outcome in sepsis. Previously we showed that meldonium, an anti-ischemic drug clinically used to treat myocardial and cerebral ischemia, strongly increases mortality in faecal-induced peritonitis (FIP) in rats. We postulated that the same mechanism that is responsible for the otherwise strong anti-inflammatory effects of meldonium could be the culprit of the increased mortality. In the present study, we applied the LPS-induced model of sepsis to explore the presence of any differences from and/or similarities to the FIP model. When it comes to energy production, despite some shared similarities, it is evident that LPS and FIP models of sepsis differ greatly. A different profile of sympathoadrenal activation may account for this observation, as it was lacking in the FIP model, whereas in the LPS model it was strong enough to overcome the effects of meldonium. Therefore, choosing the appropriate model of sepsis induction is of great importance, especially if energy homeostasis is the main focus of the study. Even when differences in the experimental design of the two models are acknowledged, the role of different patterns of energy production cannot be excluded. On that account, our results draw attention to the importance of uninterrupted energy production in sepsis but also call for much-needed revisions of the current recommendations for its treatment.",
journal = "International Journal of Molecular Sciences",
title = "The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats",
volume = "23",
number = "4",
pages = "2395",
doi = "10.3390/ijms23042395"
}

Đurašević, S., Ružičić, A., Lakić, I., Tosti, T., Đurović, S., Glumac, S., Pejić, S., Todorović, A., Drakulić, D. R., Stanković, S., Jasnić, N., Đorđević, J.,& Todorović, Z.. (2022). The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats. in International Journal of Molecular Sciences, 23(4), 2395.
https://doi.org/10.3390/ijms23042395

Đurašević S, Ružičić A, Lakić I, Tosti T, Đurović S, Glumac S, Pejić S, Todorović A, Drakulić DR, Stanković S, Jasnić N, Đorđević J, Todorović Z. The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats. in International Journal of Molecular Sciences. 2022;23(4):2395.
doi:10.3390/ijms23042395 .

Đurašević, Siniša, Ružičić, Aleksandra, Lakić, Iva, Tosti, Tomislav, Đurović, Saša, Glumac, Sofija, Pejić, Snežana, Todorović, Ana, Drakulić, Dunja R., Stanković, Sanja, Jasnić, Nebojša, Đorđević, Jelena, Todorović, Zoran, "The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats" in International Journal of Molecular Sciences, 23, no. 4 (2022):2395,
https://doi.org/10.3390/ijms23042395 . .

TY  - JOUR
AU  - Pantić, Igor
AU  - Paunović, Jovana
AU  - Pejić, Snežana
AU  - Drakulić, Dunja R.
AU  - Todorović, Ana
AU  - Stanković, Sanja
AU  - Vučević, Danijela
AU  - Cumic, Jelena
AU  - Radosavljević, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10212
AB  - Artificial intelligence (AI) and machine learning models are today frequently used for classification and prediction of various biochemical processes and phenomena. In recent years, numerous research efforts have been focused on developing such models for assessment, categorization, and prediction of oxidative stress. Supervised machine learning can successfully automate the process of evaluation and quantification of oxidative damage in biological samples, as well as extract useful data from the abundance of experimental results. In this concise review, we cover the possible applications of neural networks, decision trees and regression analysis as three common strategies in machine learning. We also review recent works on the various weaknesses and limitations of artificial intelligence in biochemistry and related scientific areas. Finally, we discuss future innovative approaches on the ways how AI can contribute to the automation of oxidative stress measurement and diagnosis of diseases associated with oxidative damage.
T2  - Chemico-Biological Interactions
T2  - Chemico-Biological InteractionsChemico-Biological Interactions
T1  - Artificial intelligence approaches to the biochemistry of oxidative stress: Current state of the art
VL  - 358
SP  - 109888
DO  - 10.1016/j.cbi.2022.109888
ER  - 

@article{
author = "Pantić, Igor and Paunović, Jovana and Pejić, Snežana and Drakulić, Dunja R. and Todorović, Ana and Stanković, Sanja and Vučević, Danijela and Cumic, Jelena and Radosavljević, Tatjana",
year = "2022",
abstract = "Artificial intelligence (AI) and machine learning models are today frequently used for classification and prediction of various biochemical processes and phenomena. In recent years, numerous research efforts have been focused on developing such models for assessment, categorization, and prediction of oxidative stress. Supervised machine learning can successfully automate the process of evaluation and quantification of oxidative damage in biological samples, as well as extract useful data from the abundance of experimental results. In this concise review, we cover the possible applications of neural networks, decision trees and regression analysis as three common strategies in machine learning. We also review recent works on the various weaknesses and limitations of artificial intelligence in biochemistry and related scientific areas. Finally, we discuss future innovative approaches on the ways how AI can contribute to the automation of oxidative stress measurement and diagnosis of diseases associated with oxidative damage.",
journal = "Chemico-Biological Interactions, Chemico-Biological InteractionsChemico-Biological Interactions",
title = "Artificial intelligence approaches to the biochemistry of oxidative stress: Current state of the art",
volume = "358",
pages = "109888",
doi = "10.1016/j.cbi.2022.109888"
}

Pantić, I., Paunović, J., Pejić, S., Drakulić, D. R., Todorović, A., Stanković, S., Vučević, D., Cumic, J.,& Radosavljević, T.. (2022). Artificial intelligence approaches to the biochemistry of oxidative stress: Current state of the art. in Chemico-Biological Interactions, 358, 109888.
https://doi.org/10.1016/j.cbi.2022.109888

Pantić I, Paunović J, Pejić S, Drakulić DR, Todorović A, Stanković S, Vučević D, Cumic J, Radosavljević T. Artificial intelligence approaches to the biochemistry of oxidative stress: Current state of the art. in Chemico-Biological Interactions. 2022;358:109888.
doi:10.1016/j.cbi.2022.109888 .

Pantić, Igor, Paunović, Jovana, Pejić, Snežana, Drakulić, Dunja R., Todorović, Ana, Stanković, Sanja, Vučević, Danijela, Cumic, Jelena, Radosavljević, Tatjana, "Artificial intelligence approaches to the biochemistry of oxidative stress: Current state of the art" in Chemico-Biological Interactions, 358 (2022):109888,
https://doi.org/10.1016/j.cbi.2022.109888 . .

TY  - JOUR
AU  - Kornjača, Duško
AU  - Živković, Vladimir I.
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Marko
AU  - Stanković, Sanja
AU  - Mutavdžin, Slavica
AU  - Jakovljević, Vladimir Lj.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700041K
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7714
AB  - The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.
T2  - Archives of Biological Sciences
T1  - The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat
VL  - 70
IS  - 2
SP  - 241
EP  - 248
DO  - 10.2298/ABS170731041K
ER  - 

@article{
author = "Kornjača, Duško and Živković, Vladimir I. and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Marko and Stanković, Sanja and Mutavdžin, Slavica and Jakovljević, Vladimir Lj. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.",
journal = "Archives of Biological Sciences",
title = "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat",
volume = "70",
number = "2",
pages = "241-248",
doi = "10.2298/ABS170731041K"
}

Kornjača, D., Živković, V. I., Krstić, D. Z., Čolović, M. B., Đurić, M., Stanković, S., Mutavdžin, S., Jakovljević, V. Lj.,& Đurić, D. M.. (2018). The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences, 70(2), 241-248.
https://doi.org/10.2298/ABS170731041K

Kornjača D, Živković VI, Krstić DZ, Čolović MB, Đurić M, Stanković S, Mutavdžin S, Jakovljević VL, Đurić DM. The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences. 2018;70(2):241-248.
doi:10.2298/ABS170731041K .

Kornjača, Duško, Živković, Vladimir I., Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Marko, Stanković, Sanja, Mutavdžin, Slavica, Jakovljević, Vladimir Lj., Đurić, Dragan M., "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat" in Archives of Biological Sciences, 70, no. 2 (2018):241-248,
https://doi.org/10.2298/ABS170731041K . .

TY  - JOUR
AU  - Stanković, Sanja
AU  - Alavantić, Dragan
AU  - Majkic-Singh, Nada
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6839
AB  - Microplate array diagonal gel electrophoresis (MADGE) was invented for molecular genetic epidemiological studies. MADGE is a highly flexible, cost effective, microplate compatible solution to high throughput electrophoresis needs. It enables several thousands to million gel lines per day for direct assay of single-base variation in different capacity laboratories. Variants of the standard 96-well MADGE include: 96-well stretch-MADGE, 192-well MADGE, 384-well MADGE, and 768-well MADGE. Melt-MADGE combines the temporal thermal ramp apparatus to achieve similar throughput for de novo mutation scanning. Basic MADGE principles and procedures, preparation of MADGE gels, electrophoresis, visualization and analysis of these gels, as well as modifications of the basic 96-well MADGE will be discussed in detail. For the first time in our country, this revolutionary polyacrylamid electrophoresis was done in 1998. We shortly review our studies which used MADGE for high throughput genotyping of the apolipoprotein E. MADGE and melt-MADGE will have an important role in the future genetic research of complex diseases and especially in pharmacogenomics.
T2  - Journal of Medical Biochemistry
T1  - Madge - Microplate Array Diagonal Gel Electrophoresis
VL  - 28
IS  - 4
SP  - 235
EP  - 240
DO  - 10.2478/v10011-009-0030-y
ER  - 

@article{
author = "Stanković, Sanja and Alavantić, Dragan and Majkic-Singh, Nada",
year = "2009",
abstract = "Microplate array diagonal gel electrophoresis (MADGE) was invented for molecular genetic epidemiological studies. MADGE is a highly flexible, cost effective, microplate compatible solution to high throughput electrophoresis needs. It enables several thousands to million gel lines per day for direct assay of single-base variation in different capacity laboratories. Variants of the standard 96-well MADGE include: 96-well stretch-MADGE, 192-well MADGE, 384-well MADGE, and 768-well MADGE. Melt-MADGE combines the temporal thermal ramp apparatus to achieve similar throughput for de novo mutation scanning. Basic MADGE principles and procedures, preparation of MADGE gels, electrophoresis, visualization and analysis of these gels, as well as modifications of the basic 96-well MADGE will be discussed in detail. For the first time in our country, this revolutionary polyacrylamid electrophoresis was done in 1998. We shortly review our studies which used MADGE for high throughput genotyping of the apolipoprotein E. MADGE and melt-MADGE will have an important role in the future genetic research of complex diseases and especially in pharmacogenomics.",
journal = "Journal of Medical Biochemistry",
title = "Madge - Microplate Array Diagonal Gel Electrophoresis",
volume = "28",
number = "4",
pages = "235-240",
doi = "10.2478/v10011-009-0030-y"
}

Stanković, S., Alavantić, D.,& Majkic-Singh, N.. (2009). Madge - Microplate Array Diagonal Gel Electrophoresis. in Journal of Medical Biochemistry, 28(4), 235-240.
https://doi.org/10.2478/v10011-009-0030-y

Stanković S, Alavantić D, Majkic-Singh N. Madge - Microplate Array Diagonal Gel Electrophoresis. in Journal of Medical Biochemistry. 2009;28(4):235-240.
doi:10.2478/v10011-009-0030-y .

Stanković, Sanja, Alavantić, Dragan, Majkic-Singh, Nada, "Madge - Microplate Array Diagonal Gel Electrophoresis" in Journal of Medical Biochemistry, 28, no. 4 (2009):235-240,
https://doi.org/10.2478/v10011-009-0030-y . .

TY  - JOUR
AU  - Stanković, Aleksandra
AU  - Stanković, Sanja
AU  - Jovanovic-Markovic, Zagorka
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Glišić, Sanja
AU  - Alavantić, Dragan
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3419
AB  - The plasma concentration of apoB has recently been reported to be the best lipid predictor of coronary heart disease. The possible associations of genetic markers in the apolipoprotein B gene (Xbal, EcoRI, MspI, Ins/Del, and 4311 A/G polymorphisms) were evaluated in patients with ischemic cerebrovascular disease (ICVD) and controls of equivalent BMI. The odds ratio for ICVD in the X+X+ genotype was 2.22, 95% CI 1.24-3.96 (P LT 0.05), while that for ICVD in the Ins/Ins genotype was 2.82, 95% CI 1.57-5.06 (P LT 0.05). The patients had significantly higher frequency of the 4311 A allele compared to the controls (P LT 0.01). Our results support the assumption that apoB gene polymorphisms may contribute to the extent of cerebrovascular disease risk.
T2  - Archives of biological sciences
T1  - Apolipoprotein B gene polymorphisms in patients from Serbia with ischemic cerebrovascular disease
VL  - 59
IS  - 4
SP  - 303
EP  - 309
DO  - 10.2298/ABS0704303S
ER  - 

@article{
author = "Stanković, Aleksandra and Stanković, Sanja and Jovanovic-Markovic, Zagorka and Živković, Maja and Đurić, Tamara and Glišić, Sanja and Alavantić, Dragan",
year = "2007",
abstract = "The plasma concentration of apoB has recently been reported to be the best lipid predictor of coronary heart disease. The possible associations of genetic markers in the apolipoprotein B gene (Xbal, EcoRI, MspI, Ins/Del, and 4311 A/G polymorphisms) were evaluated in patients with ischemic cerebrovascular disease (ICVD) and controls of equivalent BMI. The odds ratio for ICVD in the X+X+ genotype was 2.22, 95% CI 1.24-3.96 (P LT 0.05), while that for ICVD in the Ins/Ins genotype was 2.82, 95% CI 1.57-5.06 (P LT 0.05). The patients had significantly higher frequency of the 4311 A allele compared to the controls (P LT 0.01). Our results support the assumption that apoB gene polymorphisms may contribute to the extent of cerebrovascular disease risk.",
journal = "Archives of biological sciences",
title = "Apolipoprotein B gene polymorphisms in patients from Serbia with ischemic cerebrovascular disease",
volume = "59",
number = "4",
pages = "303-309",
doi = "10.2298/ABS0704303S"
}

Stanković, A., Stanković, S., Jovanovic-Markovic, Z., Živković, M., Đurić, T., Glišić, S.,& Alavantić, D.. (2007). Apolipoprotein B gene polymorphisms in patients from Serbia with ischemic cerebrovascular disease. in Archives of biological sciences, 59(4), 303-309.
https://doi.org/10.2298/ABS0704303S

Stanković A, Stanković S, Jovanovic-Markovic Z, Živković M, Đurić T, Glišić S, Alavantić D. Apolipoprotein B gene polymorphisms in patients from Serbia with ischemic cerebrovascular disease. in Archives of biological sciences. 2007;59(4):303-309.
doi:10.2298/ABS0704303S .

Stanković, Aleksandra, Stanković, Sanja, Jovanovic-Markovic, Zagorka, Živković, Maja, Đurić, Tamara, Glišić, Sanja, Alavantić, Dragan, "Apolipoprotein B gene polymorphisms in patients from Serbia with ischemic cerebrovascular disease" in Archives of biological sciences, 59, no. 4 (2007):303-309,
https://doi.org/10.2298/ABS0704303S . .