TY  - JOUR
AU  - Keta, Otilija D.
AU  - Deljanin, Milena
AU  - Petković, Vladana
AU  - Zdunić, Gordana
AU  - Janković, Teodora
AU  - Živković, Jelena
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Šavikin, Katarina
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8996
AB  - The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.
T2  - Records of Natural Products
T1  - Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines
VL  - 14
IS  - 5
SP  - 326
EP  - 339
DO  - 10.25135/rnp.170.19.11.1477
ER  - 

@article{
author = "Keta, Otilija D. and Deljanin, Milena and Petković, Vladana and Zdunić, Gordana and Janković, Teodora and Živković, Jelena and Ristić-Fira, Aleksandra and Petrović, Ivan M. and Šavikin, Katarina",
year = "2020",
abstract = "The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.",
journal = "Records of Natural Products",
title = "Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines",
volume = "14",
number = "5",
pages = "326-339",
doi = "10.25135/rnp.170.19.11.1477"
}

Keta, O. D., Deljanin, M., Petković, V., Zdunić, G., Janković, T., Živković, J., Ristić-Fira, A., Petrović, I. M.,& Šavikin, K.. (2020). Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines. in Records of Natural Products, 14(5), 326-339.
https://doi.org/10.25135/rnp.170.19.11.1477

Keta OD, Deljanin M, Petković V, Zdunić G, Janković T, Živković J, Ristić-Fira A, Petrović IM, Šavikin K. Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines. in Records of Natural Products. 2020;14(5):326-339.
doi:10.25135/rnp.170.19.11.1477 .

Keta, Otilija D., Deljanin, Milena, Petković, Vladana, Zdunić, Gordana, Janković, Teodora, Živković, Jelena, Ristić-Fira, Aleksandra, Petrović, Ivan M., Šavikin, Katarina, "Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines" in Records of Natural Products, 14, no. 5 (2020):326-339,
https://doi.org/10.25135/rnp.170.19.11.1477 . .

TY  - JOUR
AU  - Janković, Teodora
AU  - Savikin, Katarina
AU  - Menkovic, Nebojsa
AU  - Aljancic, Ivana
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Joksić, Gordana
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3482
AB  - The aim of this study was to identify active principles of Gentianella austriaca responsible for the reduction of the incidence of micronuclei in irradiated lymphocytes in vitro. The radioprotective effects of ether (EF) and methanolic (MeF) fractions, water-soluble xanthones demethylbellidifolin (1), demethylbellidifolin 8-O-glucoside (2), bellidifolin 8-O-glucoside (3), and flavonoid swertisin (4) against chromosomal damage induced by gamma-rays were determined using the micronucleus test. EF and MeF showed better protection in treatment of human lymphocytes after gamma-irradiation than did isolated compounds. Among the isolated compounds, the effectivenessin reduction of the frequency of micronuclei followed the order 4 GT 3 GT 2 GT 1. The anti-lipoperoxidant activity was in the order 2 GT 4 GT 1, while 3 slightly increased the level of malondialdehyde. In addition, the effectiveness in induction of apoptosis followed the order, 3 GT 2 GT 4, while 1 had no proapoptotic effect. These results suggest that the antioxidative properties of the polyphenols tested may contribute to the radioprotective effects of G. austriaca.
T2  - Planta Medica
T1  - Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes
VL  - 74
IS  - 7
SP  - 736
EP  - 740
DO  - 10.1055/s-2008-1074524
ER  - 

@article{
author = "Janković, Teodora and Savikin, Katarina and Menkovic, Nebojsa and Aljancic, Ivana and Leskovac, Andreja and Petrović, Sandra and Joksić, Gordana",
year = "2008",
abstract = "The aim of this study was to identify active principles of Gentianella austriaca responsible for the reduction of the incidence of micronuclei in irradiated lymphocytes in vitro. The radioprotective effects of ether (EF) and methanolic (MeF) fractions, water-soluble xanthones demethylbellidifolin (1), demethylbellidifolin 8-O-glucoside (2), bellidifolin 8-O-glucoside (3), and flavonoid swertisin (4) against chromosomal damage induced by gamma-rays were determined using the micronucleus test. EF and MeF showed better protection in treatment of human lymphocytes after gamma-irradiation than did isolated compounds. Among the isolated compounds, the effectivenessin reduction of the frequency of micronuclei followed the order 4 GT 3 GT 2 GT 1. The anti-lipoperoxidant activity was in the order 2 GT 4 GT 1, while 3 slightly increased the level of malondialdehyde. In addition, the effectiveness in induction of apoptosis followed the order, 3 GT 2 GT 4, while 1 had no proapoptotic effect. These results suggest that the antioxidative properties of the polyphenols tested may contribute to the radioprotective effects of G. austriaca.",
journal = "Planta Medica",
title = "Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes",
volume = "74",
number = "7",
pages = "736-740",
doi = "10.1055/s-2008-1074524"
}

Janković, T., Savikin, K., Menkovic, N., Aljancic, I., Leskovac, A., Petrović, S.,& Joksić, G.. (2008). Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes. in Planta Medica, 74(7), 736-740.
https://doi.org/10.1055/s-2008-1074524

Janković T, Savikin K, Menkovic N, Aljancic I, Leskovac A, Petrović S, Joksić G. Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes. in Planta Medica. 2008;74(7):736-740.
doi:10.1055/s-2008-1074524 .

Janković, Teodora, Savikin, Katarina, Menkovic, Nebojsa, Aljancic, Ivana, Leskovac, Andreja, Petrović, Sandra, Joksić, Gordana, "Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes" in Planta Medica, 74, no. 7 (2008):736-740,
https://doi.org/10.1055/s-2008-1074524 . .

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Joksić, Gordana
AU  - Janković, Teodora
AU  - Savildn, Katarina
AU  - Menkovic, Nebojsa
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3302
AB  - The objective of the present study is to evaluate the radioprotective properties of extracts of Crataegus monogyna Jacq. (Rosaceae) fruit, Cornus mas L. (Cornaceae) leaves and Gentianella austriaca (A. Kern. and Jos. Kern.) Holub (Gentianaceae) aerial parts on cultured human peripheral blood lymphocytes in vitro after irradiation with 2 Gy of Co-60 gamma-rays. Plants were collected at Mt. Maljen in Serbia, air-dried and powdered, and the total phenolic content was analyzed. In C. mas leaves, ellagic and gallic acid were found to be the dominant compounds, whereas C. monogyna fruit was rich in procyanidins and flavonoids. The main constituents of G. austriaca aerial parts were gamma-pyrones and secoiridoids. Cell cultures were treated with four different doses of plant extracts (0.1 mg/mL to 0.4 mg/mL). Treatment with the lowest dose gave the best protective effect. Significant radiorecovery potentials of C. mas and C. monogyna were observed, seen as a reduced incidence of radiation-induced micronuclei (19.23 % and 13.24 %, respectively), reduced levels of lipid peroxidation products (50.04% and 13.18%, respectively) and two-fold enhanced apoptosis. Both extracts slowed down cell proliferation gradually, enabling more time for repair. G. austriaca possesses strong antioxidant properties, significantly reducing lipid peroxidation and the incidence of micronuclei (for 30.88 % and 35.56 %, respectively) while enhancing apoptosis with no perturbation of the cell cycle. This study may contribute to the search for novel radioprotective agents.
T2  - Planta Medica
T1  - Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro
VL  - 73
IS  - 11
SP  - 1169
EP  - 1175
DO  - 10.1055/s-2007-981586
ER  - 

@article{
author = "Leskovac, Andreja and Joksić, Gordana and Janković, Teodora and Savildn, Katarina and Menkovic, Nebojsa",
year = "2007",
abstract = "The objective of the present study is to evaluate the radioprotective properties of extracts of Crataegus monogyna Jacq. (Rosaceae) fruit, Cornus mas L. (Cornaceae) leaves and Gentianella austriaca (A. Kern. and Jos. Kern.) Holub (Gentianaceae) aerial parts on cultured human peripheral blood lymphocytes in vitro after irradiation with 2 Gy of Co-60 gamma-rays. Plants were collected at Mt. Maljen in Serbia, air-dried and powdered, and the total phenolic content was analyzed. In C. mas leaves, ellagic and gallic acid were found to be the dominant compounds, whereas C. monogyna fruit was rich in procyanidins and flavonoids. The main constituents of G. austriaca aerial parts were gamma-pyrones and secoiridoids. Cell cultures were treated with four different doses of plant extracts (0.1 mg/mL to 0.4 mg/mL). Treatment with the lowest dose gave the best protective effect. Significant radiorecovery potentials of C. mas and C. monogyna were observed, seen as a reduced incidence of radiation-induced micronuclei (19.23 % and 13.24 %, respectively), reduced levels of lipid peroxidation products (50.04% and 13.18%, respectively) and two-fold enhanced apoptosis. Both extracts slowed down cell proliferation gradually, enabling more time for repair. G. austriaca possesses strong antioxidant properties, significantly reducing lipid peroxidation and the incidence of micronuclei (for 30.88 % and 35.56 %, respectively) while enhancing apoptosis with no perturbation of the cell cycle. This study may contribute to the search for novel radioprotective agents.",
journal = "Planta Medica",
title = "Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro",
volume = "73",
number = "11",
pages = "1169-1175",
doi = "10.1055/s-2007-981586"
}

Leskovac, A., Joksić, G., Janković, T., Savildn, K.,& Menkovic, N.. (2007). Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro. in Planta Medica, 73(11), 1169-1175.
https://doi.org/10.1055/s-2007-981586

Leskovac A, Joksić G, Janković T, Savildn K, Menkovic N. Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro. in Planta Medica. 2007;73(11):1169-1175.
doi:10.1055/s-2007-981586 .

Leskovac, Andreja, Joksić, Gordana, Janković, Teodora, Savildn, Katarina, Menkovic, Nebojsa, "Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro" in Planta Medica, 73, no. 11 (2007):1169-1175,
https://doi.org/10.1055/s-2007-981586 . .

TY  - CONF
AU  - Leskovac, Andreja
AU  - Momić, Tatjana
AU  - Janković, Teodora
AU  - Joksić, Gordana
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9504
AB  - The aim of this study was to evaluate radioprotective properties of medical plants Gentianella austriaca and Gentianella dinarica. For this purpose human lymphocytes were irradiated using 60Co γ rays and treated with different fractions of plant extracts, afterwards micronuclei (MN) and malondialdehyde (MDA) were measured. Polyphenols, fractions isolated from those plants have shown the protective effects, seen as significantly reduced incidence of micronuclei which was followed with reduced level of malondialdehyde. The results obtained in this study indicate that polyphenols isolated from Gentianella austriaca and Gentianella dinarica posses radioprotective properties possibly trough reduction of the lipid peroxidation.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - The radioprotective properties of polyphenols on human lymphocytes
VL  - 1
SP  - 410
EP  - 412
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9504
ER  - 

@conference{
author = "Leskovac, Andreja and Momić, Tatjana and Janković, Teodora and Joksić, Gordana",
year = "2004",
abstract = "The aim of this study was to evaluate radioprotective properties of medical plants Gentianella austriaca and Gentianella dinarica. For this purpose human lymphocytes were irradiated using 60Co γ rays and treated with different fractions of plant extracts, afterwards micronuclei (MN) and malondialdehyde (MDA) were measured. Polyphenols, fractions isolated from those plants have shown the protective effects, seen as significantly reduced incidence of micronuclei which was followed with reduced level of malondialdehyde. The results obtained in this study indicate that polyphenols isolated from Gentianella austriaca and Gentianella dinarica posses radioprotective properties possibly trough reduction of the lipid peroxidation.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "The radioprotective properties of polyphenols on human lymphocytes",
volume = "1",
pages = "410-412",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9504"
}

Leskovac, A., Momić, T., Janković, T.,& Joksić, G.. (2004). The radioprotective properties of polyphenols on human lymphocytes. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 410-412.
https://hdl.handle.net/21.15107/rcub_vinar_9504

Leskovac A, Momić T, Janković T, Joksić G. The radioprotective properties of polyphenols on human lymphocytes. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:410-412.
https://hdl.handle.net/21.15107/rcub_vinar_9504 .

Leskovac, Andreja, Momić, Tatjana, Janković, Teodora, Joksić, Gordana, "The radioprotective properties of polyphenols on human lymphocytes" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):410-412,
https://hdl.handle.net/21.15107/rcub_vinar_9504 .

TY  - JOUR
AU  - Todorović, Vera
AU  - Janić, B.
AU  - Koko, Vesna
AU  - Micev, Marjan T.
AU  - Nikolić, Judith Anna
AU  - Ratković, Marija
AU  - Leposavić, Gordana
AU  - Janković, Teodora
AU  - Knežević-Ušaj, Slavica
AU  - Milićević, Zorka T.
PY  - 1996
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1990
AB  - Background/Aims: In this study, we present radioimmunoassay data describing the concentration of Vasoactive Intestinal Polypeptide (VIP) in both plasma and colonic biopsies, as well as immunostaining of VIPergic innervation in mucosal biopsies of normal subjects and patients with ulcerative colitis (UC). Patients and Methods: Thirty three patients with UC and 17 healthy subjects were investigated. All UC patients suffered from active disease. Fasting circulating levels of VIP in plasma as well as tissue concentrations were measured by radioimmunoassay. For the immunohistochemistry, polyclonal antibody against VIP and the streptavidin-biotin peroxidase complex technique were carried out. Results: Overall plasma VIP concentrations in the UC patients were similar to those in the controls. Significantly decreased concentrations of VIP were found in UC of rectum compared to the normal tissue. However, both plasma VIP values and tissue concentrations were found to be significantly lower in patients expressing minimal or mild active disease according to clinical activity index (AI) and histological activity index (HAI), but marked increase of plasma VIP was clear in UC patients with moderate or severe AI and HAI. There was a trend towards increased tissue concentrations of VIP in the group of patients with moderate or severe AI and HAI. Our immunohistochemical analysis of VIP fibers and nerve cell bodies revealed consistently weaker VIP-immunoreactivity in the rectum in UC patients with minimal or mild HAI. Simultaneously, in the rectal biopsies from UC patients with moderate and severe disease, the fibers in the lamina propria and ganglion cells in the submucous plexus were markedly increased in density and in degree of immunostaining. Very strong immunoreactivity was also found in inflammatory cells of the lamina propria as well as in the epithelial layer of the biopsies from UC patients with obvious disease. Conclusions: Our study shows clearly the heterogeneity in the response of VIP plasma level as well as rectum concentration and distribution in UC patients at different stages of the active disease. The possible role of VIP in the VIP in the colon suggests that further studies of the alterations of this gut peptide may be useful in the understanding of UC pathophysiology.
T2  - Hepato-gastroenterology
T1  - Colonic Vasoactive Intestinal Polypeptide (VIP) in ulcerative colitis - A radioimmunoassay and immunohistochemical study
VL  - 43
IS  - 9
SP  - 483
EP  - 488
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1990
ER  - 

@article{
author = "Todorović, Vera and Janić, B. and Koko, Vesna and Micev, Marjan T. and Nikolić, Judith Anna and Ratković, Marija and Leposavić, Gordana and Janković, Teodora and Knežević-Ušaj, Slavica and Milićević, Zorka T.",
year = "1996",
abstract = "Background/Aims: In this study, we present radioimmunoassay data describing the concentration of Vasoactive Intestinal Polypeptide (VIP) in both plasma and colonic biopsies, as well as immunostaining of VIPergic innervation in mucosal biopsies of normal subjects and patients with ulcerative colitis (UC). Patients and Methods: Thirty three patients with UC and 17 healthy subjects were investigated. All UC patients suffered from active disease. Fasting circulating levels of VIP in plasma as well as tissue concentrations were measured by radioimmunoassay. For the immunohistochemistry, polyclonal antibody against VIP and the streptavidin-biotin peroxidase complex technique were carried out. Results: Overall plasma VIP concentrations in the UC patients were similar to those in the controls. Significantly decreased concentrations of VIP were found in UC of rectum compared to the normal tissue. However, both plasma VIP values and tissue concentrations were found to be significantly lower in patients expressing minimal or mild active disease according to clinical activity index (AI) and histological activity index (HAI), but marked increase of plasma VIP was clear in UC patients with moderate or severe AI and HAI. There was a trend towards increased tissue concentrations of VIP in the group of patients with moderate or severe AI and HAI. Our immunohistochemical analysis of VIP fibers and nerve cell bodies revealed consistently weaker VIP-immunoreactivity in the rectum in UC patients with minimal or mild HAI. Simultaneously, in the rectal biopsies from UC patients with moderate and severe disease, the fibers in the lamina propria and ganglion cells in the submucous plexus were markedly increased in density and in degree of immunostaining. Very strong immunoreactivity was also found in inflammatory cells of the lamina propria as well as in the epithelial layer of the biopsies from UC patients with obvious disease. Conclusions: Our study shows clearly the heterogeneity in the response of VIP plasma level as well as rectum concentration and distribution in UC patients at different stages of the active disease. The possible role of VIP in the VIP in the colon suggests that further studies of the alterations of this gut peptide may be useful in the understanding of UC pathophysiology.",
journal = "Hepato-gastroenterology",
title = "Colonic Vasoactive Intestinal Polypeptide (VIP) in ulcerative colitis - A radioimmunoassay and immunohistochemical study",
volume = "43",
number = "9",
pages = "483-488",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1990"
}

Todorović, V., Janić, B., Koko, V., Micev, M. T., Nikolić, J. A., Ratković, M., Leposavić, G., Janković, T., Knežević-Ušaj, S.,& Milićević, Z. T.. (1996). Colonic Vasoactive Intestinal Polypeptide (VIP) in ulcerative colitis - A radioimmunoassay and immunohistochemical study. in Hepato-gastroenterology, 43(9), 483-488.
https://hdl.handle.net/21.15107/rcub_vinar_1990

Todorović V, Janić B, Koko V, Micev MT, Nikolić JA, Ratković M, Leposavić G, Janković T, Knežević-Ušaj S, Milićević ZT. Colonic Vasoactive Intestinal Polypeptide (VIP) in ulcerative colitis - A radioimmunoassay and immunohistochemical study. in Hepato-gastroenterology. 1996;43(9):483-488.
https://hdl.handle.net/21.15107/rcub_vinar_1990 .

Todorović, Vera, Janić, B., Koko, Vesna, Micev, Marjan T., Nikolić, Judith Anna, Ratković, Marija, Leposavić, Gordana, Janković, Teodora, Knežević-Ušaj, Slavica, Milićević, Zorka T., "Colonic Vasoactive Intestinal Polypeptide (VIP) in ulcerative colitis - A radioimmunoassay and immunohistochemical study" in Hepato-gastroenterology, 43, no. 9 (1996):483-488,
https://hdl.handle.net/21.15107/rcub_vinar_1990 .