TY  - CONF
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Jovanović, N.
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9321
AB  - In this study the Ca2+ ion flux modulation in the synaptosomal mitochondria
isolated from caudate nucleus (CN) of the ovariectomised rats was examined.
17 beta-estradiol (E2), E2-conjugated to bovine serum albumin (E-BSA), estradiol
receptor α (ERα) agonist 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol
(PPT), ERβ agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DNP) and ERα/β
antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-
triene-3,17-diol (ICI 182,780) were used. The Ca2+ efflux inhibition of about 27%
was detected in the presence of 0.5 nmol/l E2, and of about 20% in the case of
E-BSA. DNP (10 nmol/l) was as much potent Ca2+ efflux inhibitor as E2, while
PPT (10 nmol/l) hardly had any inhibitory effect (9% efflux decrease). When E2
binding to ERα and ERβ was prevented by 1 μmol/l ICI 182,780, the Ca2+ efflux
inhibition of about 15% was detected. Our results suggest that E2 prevents Ca2+
efflux from synaptosomal mitochondria due to ERβ activation rather than ERα.
The involvement of the external E2 binding site on the mitochondrial membrane
probably different from ERα/β should not be excluded because of Ca2+ efflux
inhibition detected in the presence of E-BSA and ICI 182,780. The Ca2+ efflux
modulation could be the mechanism through which E2 exerts its neuromodulatory
role in specific brain structures.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9321
ER  - 

@conference{
author = "Petrović, Snježana and Milošević, Maja and Stanojević, Ivana and Drakulić, Dunja R. and Jovanović, N. and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "In this study the Ca2+ ion flux modulation in the synaptosomal mitochondria
isolated from caudate nucleus (CN) of the ovariectomised rats was examined.
17 beta-estradiol (E2), E2-conjugated to bovine serum albumin (E-BSA), estradiol
receptor α (ERα) agonist 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol
(PPT), ERβ agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DNP) and ERα/β
antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-
triene-3,17-diol (ICI 182,780) were used. The Ca2+ efflux inhibition of about 27%
was detected in the presence of 0.5 nmol/l E2, and of about 20% in the case of
E-BSA. DNP (10 nmol/l) was as much potent Ca2+ efflux inhibitor as E2, while
PPT (10 nmol/l) hardly had any inhibitory effect (9% efflux decrease). When E2
binding to ERα and ERβ was prevented by 1 μmol/l ICI 182,780, the Ca2+ efflux
inhibition of about 15% was detected. Our results suggest that E2 prevents Ca2+
efflux from synaptosomal mitochondria due to ERβ activation rather than ERα.
The involvement of the external E2 binding site on the mitochondrial membrane
probably different from ERα/β should not be excluded because of Ca2+ efflux
inhibition detected in the presence of E-BSA and ICI 182,780. The Ca2+ efflux
modulation could be the mechanism through which E2 exerts its neuromodulatory
role in specific brain structures.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9321"
}

Petrović, S., Milošević, M., Stanojević, I., Drakulić, D. R., Jovanović, N., Veličković, N.,& Horvat, A.. (2010). Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9321

Petrović S, Milošević M, Stanojević I, Drakulić DR, Jovanović N, Veličković N, Horvat A. Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9321 .

Petrović, Snježana, Milošević, Maja, Stanojević, Ivana, Drakulić, Dunja R., Jovanović, N., Veličković, Nataša, Horvat, Anica, "Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9321 .