Ajduković, Zorica

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  • Ajduković, Zorica (22)
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Author's Bibliography

Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues

Ignjatović, Nenad L.; Wu, Victoria; Ajduković, Zorica; Mihajilov Krstev, Tatjana; Uskoković, Vuk; Uskoković, Dragan

(2016)

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Wu, Victoria
AU  - Ajduković, Zorica
AU  - Mihajilov Krstev, Tatjana
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7573
AB  - Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.
T2  - Materials science & engineering. C, Materials for biological applications
T1  - Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues
VL  - 60
SP  - 357
EP  - 364
DO  - 10.1016/j.msec.2015.11.061
ER  - 
@article{
author = "Ignjatović, Nenad L. and Wu, Victoria and Ajduković, Zorica and Mihajilov Krstev, Tatjana and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7573",
abstract = "Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.",
journal = "Materials science & engineering. C, Materials for biological applications",
title = "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues",
volume = "60",
pages = "357-364",
doi = "10.1016/j.msec.2015.11.061"
}
Ignjatović, N. L., Wu, V., Ajduković, Z., Mihajilov Krstev, T., Uskoković, V.,& Uskoković, D. (2016). Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues.
Materials science & engineering. C, Materials for biological applications, 60, 357-364.
https://doi.org/10.1016/j.msec.2015.11.061
Ignjatović NL, Wu V, Ajduković Z, Mihajilov Krstev T, Uskoković V, Uskoković D. Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. Materials science & engineering. C, Materials for biological applications. 2016;60:357-364
Ignjatović Nenad L., Wu Victoria, Ajduković Zorica, Mihajilov Krstev Tatjana, Uskoković Vuk, Uskoković Dragan, "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues" Materials science & engineering. C, Materials for biological applications, 60 (2016):357-364,
https://doi.org/10.1016/j.msec.2015.11.061 .
57
49
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Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues

Ignjatović, Nenad L.; Wu, Victoria; Ajduković, Zorica; Mihajilov-Krstev, Tatjana; Uskoković, Vuk; Uskoković, Dragan

(Elsevier, 2016)

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Wu, Victoria
AU  - Ajduković, Zorica
AU  - Mihajilov-Krstev, Tatjana
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7570
AB  - Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues
VL  - 60
SP  - 357
EP  - 364
DO  - 10.1016/j.msec.2015.11.061
ER  - 
@article{
author = "Ignjatović, Nenad L. and Wu, Victoria and Ajduković, Zorica and Mihajilov-Krstev, Tatjana and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7570",
abstract = "Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues",
volume = "60",
pages = "357-364",
doi = "10.1016/j.msec.2015.11.061"
}
Ignjatović, N. L., Wu, V., Ajduković, Z., Mihajilov-Krstev, T., Uskoković, V.,& Uskoković, D. (2016). Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues.
Materials Science and Engineering C
Elsevier., 60, 357-364.
https://doi.org/10.1016/j.msec.2015.11.061
Ignjatović NL, Wu V, Ajduković Z, Mihajilov-Krstev T, Uskoković V, Uskoković D. Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. Materials Science and Engineering C. 2016;60:357-364
Ignjatović Nenad L., Wu Victoria, Ajduković Zorica, Mihajilov-Krstev Tatjana, Uskoković Vuk, Uskoković Dragan, "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues" Materials Science and Engineering C, 60 (2016):357-364,
https://doi.org/10.1016/j.msec.2015.11.061 .
57
49
53

Rapid bone regeneration with nano-hydroxyapatite coated with a chitosan-poly (D, L)-lactide-co-glycolide bone-filling material with osteocondactive and antimicrobial properties

Ignjatović, Nenad L.; Uskoković, Vuk; Ajduković, Zorica; Mihajlov Krstev, Tatjana; Uskoković, Dragan

(2015)

TY  - CONF
AU  - Ignjatović, Nenad L.
AU  - Uskoković, Vuk
AU  - Ajduković, Zorica
AU  - Mihajlov Krstev, Tatjana
AU  - Uskoković, Dragan
PY  - 2015
UR  - http://dais.sanu.ac.rs/123456789/824
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/1104
UR  - http://www.itn.sanu.ac.rs/opus4/files/1104/Ignjatovic_ITNANO2015.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7541
AB  - Composite biomaterials based on nano-hydroxyapatite have an enormous potential for natural bone tissue reparation, filling and augmentation. Multifunctional nanoparticulate systems based on HAp coated with biocompatible and bioresorbable polymers make a separate group of filler systems in bone tissue engineering [1,2]. Chitosan has many physicochemical (reactive OH and NH2 groups) and biological (biocompatible, biodegradable) properties that make it an attractive material for use in bone tissue engineering. However, chitosan may induce thrombosis and it is therefore unsuitable as blood – contacting biomaterial. One of the strategies to improve the biocompatibility of chitosan is combination of this biopolymer with compounds that exhibit complementary properties. In our studies, we present the synthesis, characterization, in vitro and in vivo research of a particulate form of nano HAp-coated polymer systems. We synthesized nanoparticulate HAp coated with chitosan (Ch) and a chitosan-poly-D,L-lactide-co-glycolide (Ch-PLGA) polymer blend obtained via the solvent/non-solvent method and freeze-drying processing. We also examined the possibility of using Thermo-Gravimetric Analysis/Differential-Thermal Analysis (DTA/TGA) coupled on-line with mass spectrometry (MS) as a finger print for identification purposes in coating processes. The quantitative antimicrobial test has shown that HAp/Ch-PLGA have some antibacterial properties (MIC (mg/mL): Pseudomonas aeruginosa – 6.40, Staphylococcus aureus – 6.40, Staphylococcus epidermidis – 3.20). MTT assay was used to test cytotoxicity and cell viability. By using HAp/Ch-PLGA in the form of a filler a high level of reparatory ability, with the presence Haversian canals and cement lines in reconstructed of bone defect, was achieved in vivo. [1] N. Ignjatovic, C. Liu, J. Czernuszka, D. Uskokovic, Micro and nano/injectable composite biomaterials containing calcium phosphate coated with poly(dl-lactide-co-glycolide), Acta Biomaterialia, 3 (2007) 927-935 [2] N. Ignjatović, V. Uskoković, Z. Ajduković, D. Uskoković, Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol, Materials Science and Engineering: C 33 (2013) 943–950
C3  - ITNANO2015: 3RD International Translational Nanomedicine Conference  21-26. June. 2015, Milocer, Hotel Maestral, Montenegro: Book of abstracts
T1  - Rapid bone regeneration with nano-hydroxyapatite coated with a chitosan-poly (D, L)-lactide-co-glycolide bone-filling material with osteocondactive and antimicrobial properties
SP  - 15
EP  - 16
ER  - 
@conference{
author = "Ignjatović, Nenad L. and Uskoković, Vuk and Ajduković, Zorica and Mihajlov Krstev, Tatjana and Uskoković, Dragan",
year = "2015",
url = "http://dais.sanu.ac.rs/123456789/824, http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/1104, http://www.itn.sanu.ac.rs/opus4/files/1104/Ignjatovic_ITNANO2015.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7541",
abstract = "Composite biomaterials based on nano-hydroxyapatite have an enormous potential for natural bone tissue reparation, filling and augmentation. Multifunctional nanoparticulate systems based on HAp coated with biocompatible and bioresorbable polymers make a separate group of filler systems in bone tissue engineering [1,2]. Chitosan has many physicochemical (reactive OH and NH2 groups) and biological (biocompatible, biodegradable) properties that make it an attractive material for use in bone tissue engineering. However, chitosan may induce thrombosis and it is therefore unsuitable as blood – contacting biomaterial. One of the strategies to improve the biocompatibility of chitosan is combination of this biopolymer with compounds that exhibit complementary properties. In our studies, we present the synthesis, characterization, in vitro and in vivo research of a particulate form of nano HAp-coated polymer systems. We synthesized nanoparticulate HAp coated with chitosan (Ch) and a chitosan-poly-D,L-lactide-co-glycolide (Ch-PLGA) polymer blend obtained via the solvent/non-solvent method and freeze-drying processing. We also examined the possibility of using Thermo-Gravimetric Analysis/Differential-Thermal Analysis (DTA/TGA) coupled on-line with mass spectrometry (MS) as a finger print for identification purposes in coating processes. The quantitative antimicrobial test has shown that HAp/Ch-PLGA have some antibacterial properties (MIC (mg/mL): Pseudomonas aeruginosa – 6.40, Staphylococcus aureus – 6.40, Staphylococcus epidermidis – 3.20). MTT assay was used to test cytotoxicity and cell viability. By using HAp/Ch-PLGA in the form of a filler a high level of reparatory ability, with the presence Haversian canals and cement lines in reconstructed of bone defect, was achieved in vivo. [1] N. Ignjatovic, C. Liu, J. Czernuszka, D. Uskokovic, Micro and nano/injectable composite biomaterials containing calcium phosphate coated with poly(dl-lactide-co-glycolide), Acta Biomaterialia, 3 (2007) 927-935 [2] N. Ignjatović, V. Uskoković, Z. Ajduković, D. Uskoković, Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol, Materials Science and Engineering: C 33 (2013) 943–950",
journal = "ITNANO2015: 3RD International Translational Nanomedicine Conference  21-26. June. 2015, Milocer, Hotel Maestral, Montenegro: Book of abstracts",
title = "Rapid bone regeneration with nano-hydroxyapatite coated with a chitosan-poly (D, L)-lactide-co-glycolide bone-filling material with osteocondactive and antimicrobial properties",
pages = "15-16"
}
Ignjatović, N. L., Uskoković, V., Ajduković, Z., Mihajlov Krstev, T.,& Uskoković, D. (2015). Rapid bone regeneration with nano-hydroxyapatite coated with a chitosan-poly (D, L)-lactide-co-glycolide bone-filling material with osteocondactive and antimicrobial properties.
ITNANO2015: 3RD International Translational Nanomedicine Conference  21-26. June. 2015, Milocer, Hotel Maestral, Montenegro: Book of abstracts, 15-16.
Ignjatović NL, Uskoković V, Ajduković Z, Mihajlov Krstev T, Uskoković D. Rapid bone regeneration with nano-hydroxyapatite coated with a chitosan-poly (D, L)-lactide-co-glycolide bone-filling material with osteocondactive and antimicrobial properties. ITNANO2015: 3RD International Translational Nanomedicine Conference  21-26. June. 2015, Milocer, Hotel Maestral, Montenegro: Book of abstracts. 2015;:15-16
Ignjatović Nenad L., Uskoković Vuk, Ajduković Zorica, Mihajlov Krstev Tatjana, Uskoković Dragan, "Rapid bone regeneration with nano-hydroxyapatite coated with a chitosan-poly (D, L)-lactide-co-glycolide bone-filling material with osteocondactive and antimicrobial properties" ITNANO2015: 3RD International Translational Nanomedicine Conference  21-26. June. 2015, Milocer, Hotel Maestral, Montenegro: Book of abstracts (2015):15-16

Interaction of nanoparticles and biological fluids

Ajduković, Zorica; Ignjatović, Nenad L.; Petrović, Nenad; Rajković, Jelena; Kenić Marinković, Dragana; Najman, Stevo; Mihailović, Dragan; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2014)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Petrović, Nenad
AU  - Rajković, Jelena
AU  - Kenić Marinković, Dragana
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Uskoković, Dragan
PY  - 2014
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/826
UR  - http://dais.sanu.ac.rs/123456789/558
UR  - http://www.itn.sanu.ac.rs/opus4/files/826/Ajdukovic_YUCOMAT2014.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7477
AB  - Recent studies have shown that the behavior of nanoparticles in in vivo conditions is not solely dependant on their physical properties, such are size and shape. The surface of nanoparticles in biological fluids interacts with biomolecules such are proteins, adsorbs these molecules, and leads to formation of nanoparticle-biomoelcular complex known as “protein corona”. This protein corona changes the properties of nanoparticles and their behavior in vivo. In this work three types of nanomaterial based on Hap and polymers were incubated in rats plasma, and the identification and quantification of proteins in protein corona that had formed around these nanoparticles, was done by means of electrophoresis and mass spectrophotometery.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
T1  - Interaction of nanoparticles and biological fluids
SP  - 113
EP  - 113
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad L. and Petrović, Nenad and Rajković, Jelena and Kenić Marinković, Dragana and Najman, Stevo and Mihailović, Dragan and Uskoković, Dragan",
year = "2014",
url = "http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/826, http://dais.sanu.ac.rs/123456789/558, http://www.itn.sanu.ac.rs/opus4/files/826/Ajdukovic_YUCOMAT2014.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7477",
abstract = "Recent studies have shown that the behavior of nanoparticles in in vivo conditions is not solely dependant on their physical properties, such are size and shape. The surface of nanoparticles in biological fluids interacts with biomolecules such are proteins, adsorbs these molecules, and leads to formation of nanoparticle-biomoelcular complex known as “protein corona”. This protein corona changes the properties of nanoparticles and their behavior in vivo. In this work three types of nanomaterial based on Hap and polymers were incubated in rats plasma, and the identification and quantification of proteins in protein corona that had formed around these nanoparticles, was done by means of electrophoresis and mass spectrophotometery.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts",
title = "Interaction of nanoparticles and biological fluids",
pages = "113-113"
}
Ajduković, Z., Ignjatović, N. L., Petrović, N., Rajković, J., Kenić Marinković, D., Najman, S., Mihailović, D.,& Uskoković, D. (2014). Interaction of nanoparticles and biological fluids.
The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 113-113.
Ajduković Z, Ignjatović NL, Petrović N, Rajković J, Kenić Marinković D, Najman S, Mihailović D, Uskoković D. Interaction of nanoparticles and biological fluids. The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts. 2014;:113-113
Ajduković Zorica, Ignjatović Nenad L., Petrović Nenad, Rajković Jelena, Kenić Marinković Dragana, Najman Stevo, Mihailović Dragan, Uskoković Dragan, "Interaction of nanoparticles and biological fluids" The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts (2014):113-113

Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period

Mitić, Žarko; Najman, Stevo; Cakić, Milorad; Ajduković, Zorica; Ignjatović, Nenad L.; Nikolić, Ružica; Nikolić, Goran; Stojanović, Sanja; Vukelić, Marija; Trajanović, Miroslav

(Elsevier, 2014)

TY  - JOUR
AU  - Mitić, Žarko
AU  - Najman, Stevo
AU  - Cakić, Milorad
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Nikolić, Ružica
AU  - Nikolić, Goran
AU  - Stojanović, Sanja
AU  - Vukelić, Marija
AU  - Trajanović, Miroslav
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/554
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/822
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7476
AB  - The influence of glucocorticoids on the composition and mineral/organic content of the mandible in tested animals after recovery and healing phase was investigated in this work. The results of FTIR analysis demonstrated that bone tissue composition was changed after glucocorticoid treatment. The increase of calcium, magnesium, phosphorus content and mineral part of bones was statistically significant in recovery phase and in treatment phase that included calcitonin and thymus extract. Some changes also happened in the organic part of the matrix, as indicated by intensity changes for already present IR bands and the appearance of new IR bands in the region 3500–1300 cm−1.
PB  - Elsevier
T2  - Journal of Molecular Structure
T1  - Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period
SP  - 315
EP  - 320
DO  - 10.1016/j.molstruc.2014.06.006
ER  - 
@article{
author = "Mitić, Žarko and Najman, Stevo and Cakić, Milorad and Ajduković, Zorica and Ignjatović, Nenad L. and Nikolić, Ružica and Nikolić, Goran and Stojanović, Sanja and Vukelić, Marija and Trajanović, Miroslav",
year = "2014",
url = "http://dais.sanu.ac.rs/123456789/554, http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/822, http://vinar.vin.bg.ac.rs/handle/123456789/7476",
abstract = "The influence of glucocorticoids on the composition and mineral/organic content of the mandible in tested animals after recovery and healing phase was investigated in this work. The results of FTIR analysis demonstrated that bone tissue composition was changed after glucocorticoid treatment. The increase of calcium, magnesium, phosphorus content and mineral part of bones was statistically significant in recovery phase and in treatment phase that included calcitonin and thymus extract. Some changes also happened in the organic part of the matrix, as indicated by intensity changes for already present IR bands and the appearance of new IR bands in the region 3500–1300 cm−1.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure",
title = "Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period",
pages = "315-320",
doi = "10.1016/j.molstruc.2014.06.006"
}
Mitić, Ž., Najman, S., Cakić, M., Ajduković, Z., Ignjatović, N. L., Nikolić, R., Nikolić, G., Stojanović, S., Vukelić, M.,& Trajanović, M. (2014). Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period.
Journal of Molecular Structure
Elsevier., 315-320.
https://doi.org/10.1016/j.molstruc.2014.06.006
Mitić Ž, Najman S, Cakić M, Ajduković Z, Ignjatović NL, Nikolić R, Nikolić G, Stojanović S, Vukelić M, Trajanović M. Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period. Journal of Molecular Structure. 2014;:315-320
Mitić Žarko, Najman Stevo, Cakić Milorad, Ajduković Zorica, Ignjatović Nenad L., Nikolić Ružica, Nikolić Goran, Stojanović Sanja, Vukelić Marija, Trajanović Miroslav, "Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period" Journal of Molecular Structure (2014):315-320,
https://doi.org/10.1016/j.molstruc.2014.06.006 .
5
2
5

The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment

Ignjatović, Nenad L.; Ajduković, Zorica; Vranješ-Đurić, Sanja; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2014)

TY  - CONF
AU  - Ignjatović, Nenad L.
AU  - Ajduković, Zorica
AU  - Vranješ-Đurić, Sanja
AU  - Uskoković, Dragan
PY  - 2014
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/852
UR  - http://dais.sanu.ac.rs/123456789/583
UR  - http://www.itn.sanu.ac.rs/opus4/files/852/Ignjatovic_YUCOMAT2014.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7486
AB  - Hydroxyapatite (HAp) nanoparticles coated with bioresorbable polymers have been successfully used as filler, carriers of antibiotics, vitamins and stem cells in bone tissue engineering. Interactions in the microenvironment of nanoparticulate hydroxyapatites and tissues are of a particular interest and present a challenge in regenerative medicine. The phenomena that influence the success of targeting and controlled operation or therapeutic nanosystems are numerous and complex: size, shape, surface charge, functional groups of nanoparticles, electrical double-layer formation, zeta potential, partial molar free energy, sorption molar free energy, isoelectric point of solid–liquid interface; receptor–ligand binding interactions of nano-bio interface etc. In our studies we present the synthesis, characterization, in vitro and in vivo research of nanoparticulate form of HAp-coated polymers systems. Synthesized nanoparticulate HAp coated with different types of bioresorbable polymers: poly(D,L-lactide-co-glycolide), chitozan and other similar by the solvent/non-solvent method. The physical and chemical analyses have confirmed that HAp particles are coated with bioresorbable polymers. Results of spectroscopic analysis suggests formation of hydrogen bonds between the surface groups of HAp and =O and –H groups in the polymer chain from bioresorbable polymers. By adding nanoparticulate HAp in the polymer matrix was achieved the changes in the partial molar free energy and sorption molar free energy of the system. In vitro results showed satisfactory biocompatibility of nanoparticulate HAp-coated polymers systems. The use of these systems in the form of filler was achieved a high quality osteogenesis in reconstruction of bone tissue, in vivo.The biodistribution of 125I-labeled HAp particles after intravenous injection in rats showed the potential use of these materials as a organ-targeting system: uncoated-HAp mostly targeted the liver, coated-HAp spleen and lungs.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
T1  - The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment
SP  - 40
EP  - 40
ER  - 
@conference{
author = "Ignjatović, Nenad L. and Ajduković, Zorica and Vranješ-Đurić, Sanja and Uskoković, Dragan",
year = "2014",
url = "http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/852, http://dais.sanu.ac.rs/123456789/583, http://www.itn.sanu.ac.rs/opus4/files/852/Ignjatovic_YUCOMAT2014.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7486",
abstract = "Hydroxyapatite (HAp) nanoparticles coated with bioresorbable polymers have been successfully used as filler, carriers of antibiotics, vitamins and stem cells in bone tissue engineering. Interactions in the microenvironment of nanoparticulate hydroxyapatites and tissues are of a particular interest and present a challenge in regenerative medicine. The phenomena that influence the success of targeting and controlled operation or therapeutic nanosystems are numerous and complex: size, shape, surface charge, functional groups of nanoparticles, electrical double-layer formation, zeta potential, partial molar free energy, sorption molar free energy, isoelectric point of solid–liquid interface; receptor–ligand binding interactions of nano-bio interface etc. In our studies we present the synthesis, characterization, in vitro and in vivo research of nanoparticulate form of HAp-coated polymers systems. Synthesized nanoparticulate HAp coated with different types of bioresorbable polymers: poly(D,L-lactide-co-glycolide), chitozan and other similar by the solvent/non-solvent method. The physical and chemical analyses have confirmed that HAp particles are coated with bioresorbable polymers. Results of spectroscopic analysis suggests formation of hydrogen bonds between the surface groups of HAp and =O and –H groups in the polymer chain from bioresorbable polymers. By adding nanoparticulate HAp in the polymer matrix was achieved the changes in the partial molar free energy and sorption molar free energy of the system. In vitro results showed satisfactory biocompatibility of nanoparticulate HAp-coated polymers systems. The use of these systems in the form of filler was achieved a high quality osteogenesis in reconstruction of bone tissue, in vivo.The biodistribution of 125I-labeled HAp particles after intravenous injection in rats showed the potential use of these materials as a organ-targeting system: uncoated-HAp mostly targeted the liver, coated-HAp spleen and lungs.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts",
title = "The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment",
pages = "40-40"
}
Ignjatović, N. L., Ajduković, Z., Vranješ-Đurić, S.,& Uskoković, D. (2014). The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment.
The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 40-40.
Ignjatović NL, Ajduković Z, Vranješ-Đurić S, Uskoković D. The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment. The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts. 2014;:40-40
Ignjatović Nenad L., Ajduković Zorica, Vranješ-Đurić Sanja, Uskoković Dragan, "The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment" The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts (2014):40-40

Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta

Ajduković, Zorica; Ignjatović, Nenad L.; Petrović, N.; Rajković, J.; Kenić Marinković, D.; Najman, Stevo; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2013)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Petrović, N.
AU  - Rajković, J.
AU  - Kenić Marinković, D.
AU  - Najman, Stevo
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://dais.sanu.ac.rs/123456789/388
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/649
UR  - http://www.itn.sanu.ac.rs/opus4/files/649/Ajdukovic_YUCOMAT2013_147.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7413
AB  - The lack of bone in the jaw tegmenta inflicts major problem and leads to an inability to adequately treat stomatoprosthetic patients. If the bone tissue damage is minor, the balanced activities of osteoblasts and bone osteoclasts can repair it independently. If the damage is bigger it is necessary to support the biological potential to repair the bone, and for that reason nanoparticlebiomaterial Ca / Co-HAp was used in this study. The research was done on rats with uniform anatomical and physiological characteristics. Assessment of repair and consolidation of the jaw bone tegmenta was performed by istomorphometric and SEM analysis. The best results were obtained in the experimental group of animals where the Ca / Co-Hap was mixed with autologousplasma. Following the implementation of the above mentioned nanocomposites, a significant formation of new bone was evident on the SEM analysis, as well as the rising of histomorphometric parameters of bone formation, which indicates that the Ca / Co-HAp nanocomposite is the material of choice for the rapid regeneration and repair of bone jaw tegmenta.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
T1  - Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta
SP  - 147
EP  - 147
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad L. and Petrović, N. and Rajković, J. and Kenić Marinković, D. and Najman, Stevo and Uskoković, Dragan",
year = "2013",
url = "http://dais.sanu.ac.rs/123456789/388, http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/649, http://www.itn.sanu.ac.rs/opus4/files/649/Ajdukovic_YUCOMAT2013_147.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7413",
abstract = "The lack of bone in the jaw tegmenta inflicts major problem and leads to an inability to adequately treat stomatoprosthetic patients. If the bone tissue damage is minor, the balanced activities of osteoblasts and bone osteoclasts can repair it independently. If the damage is bigger it is necessary to support the biological potential to repair the bone, and for that reason nanoparticlebiomaterial Ca / Co-HAp was used in this study. The research was done on rats with uniform anatomical and physiological characteristics. Assessment of repair and consolidation of the jaw bone tegmenta was performed by istomorphometric and SEM analysis. The best results were obtained in the experimental group of animals where the Ca / Co-Hap was mixed with autologousplasma. Following the implementation of the above mentioned nanocomposites, a significant formation of new bone was evident on the SEM analysis, as well as the rising of histomorphometric parameters of bone formation, which indicates that the Ca / Co-HAp nanocomposite is the material of choice for the rapid regeneration and repair of bone jaw tegmenta.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts",
title = "Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta",
pages = "147-147"
}
Ajduković, Z., Ignjatović, N. L., Petrović, N., Rajković, J., Kenić Marinković, D., Najman, S.,& Uskoković, D. (2013). Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta.
The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 147-147.
Ajduković Z, Ignjatović NL, Petrović N, Rajković J, Kenić Marinković D, Najman S, Uskoković D. Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta. The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:147-147
Ajduković Zorica, Ignjatović Nenad L., Petrović N., Rajković J., Kenić Marinković D., Najman Stevo, Uskoković Dragan, "Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta" The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):147-147

Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol

Ignjatović, Nenad L.; Uskoković, Vuk; Ajduković, Zorica; Uskoković, Dragan

(2013)

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Uskoković, Vuk
AU  - Ajduković, Zorica
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7571
AB  - Cholecalciferol, vitamin D3, plays an important role in bone metabolism by regulating extracellular levels of calcium. Presented here is a study on the effects of the local delivery of cholecalciferol (D3) using nanoparticulate carriers composed of hydroxyapatite (HAp) and poly(D,L-lactide-co-glycolide) (PLGA). Multifunctional nanoparticulate HAp-based powders were prepared for the purpose of: (a) either fast or sustained, local delivery of cholecalciferol, and (b) the secondary, osteoconductive and defect-filling effect of the carrier itself. Two types of HAp-based powders with particles of narrowly dispersed sizes in the nano range were prepared and tested in this study: HAp nanoparticles as direct cholecalciferol delivery agents and HAp nanoparticles coated with cholecalciferol-loaded poly(D,L)-lactide-co-glycolide (HAp/D3/PLGA).Satisfying biocompatibility of particulate systems, when incubated in contact with MC3T3-E1 osteoblastic cells in vitro, was observed for HAp/D3/PLGA and pure HAp. In contrast, an extensively fast release of cholecalciferol from the system comprising HAp nanoparticles coated with cholecalciferol (HAp/D3) triggered necrosis of the osteoblastic cells in vitro. Artificial defects induced in the osteoporotic bone of the rat mandible were successfully reconstructed following implantation of cholecalciferol-coated HAp nanoparticles as well as those comprising HAp nanoparticles coated with cholecalciferol-loaded PLGA (HAp/D3/PLGA). The greatest levels of enhanced angiogenesis, vascularization, osteogenesis and bone structure differentiation were achieved upon the implementation of HAp/D3/PLGA systems.
T2  - Materials Science and Engineering: C
T1  - Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol
VL  - 33
IS  - 2
SP  - 943
EP  - 950
DO  - 10.1016/j.msec.2012.11.026
ER  - 
@article{
author = "Ignjatović, Nenad L. and Uskoković, Vuk and Ajduković, Zorica and Uskoković, Dragan",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7571",
abstract = "Cholecalciferol, vitamin D3, plays an important role in bone metabolism by regulating extracellular levels of calcium. Presented here is a study on the effects of the local delivery of cholecalciferol (D3) using nanoparticulate carriers composed of hydroxyapatite (HAp) and poly(D,L-lactide-co-glycolide) (PLGA). Multifunctional nanoparticulate HAp-based powders were prepared for the purpose of: (a) either fast or sustained, local delivery of cholecalciferol, and (b) the secondary, osteoconductive and defect-filling effect of the carrier itself. Two types of HAp-based powders with particles of narrowly dispersed sizes in the nano range were prepared and tested in this study: HAp nanoparticles as direct cholecalciferol delivery agents and HAp nanoparticles coated with cholecalciferol-loaded poly(D,L)-lactide-co-glycolide (HAp/D3/PLGA).Satisfying biocompatibility of particulate systems, when incubated in contact with MC3T3-E1 osteoblastic cells in vitro, was observed for HAp/D3/PLGA and pure HAp. In contrast, an extensively fast release of cholecalciferol from the system comprising HAp nanoparticles coated with cholecalciferol (HAp/D3) triggered necrosis of the osteoblastic cells in vitro. Artificial defects induced in the osteoporotic bone of the rat mandible were successfully reconstructed following implantation of cholecalciferol-coated HAp nanoparticles as well as those comprising HAp nanoparticles coated with cholecalciferol-loaded PLGA (HAp/D3/PLGA). The greatest levels of enhanced angiogenesis, vascularization, osteogenesis and bone structure differentiation were achieved upon the implementation of HAp/D3/PLGA systems.",
journal = "Materials Science and Engineering: C",
title = "Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol",
volume = "33",
number = "2",
pages = "943-950",
doi = "10.1016/j.msec.2012.11.026"
}
Ignjatović, N. L., Uskoković, V., Ajduković, Z.,& Uskoković, D. (2013). Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol.
Materials Science and Engineering: C, 33(2), 943-950.
https://doi.org/10.1016/j.msec.2012.11.026
Ignjatović NL, Uskoković V, Ajduković Z, Uskoković D. Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol. Materials Science and Engineering: C. 2013;33(2):943-950
Ignjatović Nenad L., Uskoković Vuk, Ajduković Zorica, Uskoković Dragan, "Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol" Materials Science and Engineering: C, 33, no. 2 (2013):943-950,
https://doi.org/10.1016/j.msec.2012.11.026 .
41
37
43

Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones

Ignjatović, Nenad L.; Ajduković, Zorica; Savić, Vojin; Najman, Stevo; Mihailović, Dragan; Vasiljević, Perica; Stojanović, Zoran S.; Uskoković, Vuk; Uskoković, Dragan

(2013)

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Ajduković, Zorica
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Vasiljević, Perica
AU  - Stojanović, Zoran S.
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7481
AB  - Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.
T2  - Journal of Materials Science: Materials in Medicine
T1  - Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones
SP  - 343
EP  - 354
DO  - 10.1007/s10856-012-4793-1
ER  - 
@article{
author = "Ignjatović, Nenad L. and Ajduković, Zorica and Savić, Vojin and Najman, Stevo and Mihailović, Dragan and Vasiljević, Perica and Stojanović, Zoran S. and Uskoković, Vuk and Uskoković, Dragan",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7481",
abstract = "Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.",
journal = "Journal of Materials Science: Materials in Medicine",
title = "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones",
pages = "343-354",
doi = "10.1007/s10856-012-4793-1"
}
Ignjatović, N. L., Ajduković, Z., Savić, V., Najman, S., Mihailović, D., Vasiljević, P., Stojanović, Z. S., Uskoković, V.,& Uskoković, D. (2013). Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones.
Journal of Materials Science: Materials in Medicine, 343-354.
https://doi.org/10.1007/s10856-012-4793-1
Ignjatović NL, Ajduković Z, Savić V, Najman S, Mihailović D, Vasiljević P, Stojanović ZS, Uskoković V, Uskoković D. Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. Journal of Materials Science: Materials in Medicine. 2013;:343-354
Ignjatović Nenad L., Ajduković Zorica, Savić Vojin, Najman Stevo, Mihailović Dragan, Vasiljević Perica, Stojanović Zoran S., Uskoković Vuk, Uskoković Dragan, "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones" Journal of Materials Science: Materials in Medicine (2013):343-354,
https://doi.org/10.1007/s10856-012-4793-1 .
65
54
64

Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol

Ignjatović, Nenad L.; Uskoković, Vuk; Ajduković, Zorica; Uskoković, Dragan

(Elsevier, 2013)

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Uskoković, Vuk
AU  - Ajduković, Zorica
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7411
AB  - Cholecalciferol, vitamin D3, plays an important role in bone metabolism by regulating extracellular levels of calcium. Presented here is a study on the effects of the local delivery of cholecalciferol (D3) using nanoparticulate carriers composed of hydroxyapatite (HAp) and poly(d,l-lactide-co-glycolide) (PLGA). Multifunctional nanoparticulate HAp-based powders were prepared for the purpose of: (a) either fast or sustained, local delivery of cholecalciferol, and (b) the secondary, osteoconductive and defect-filling effect of the carrier itself. Two types of HAp-based powders with particles of narrowly dispersed sizes in the nano range were prepared and tested in this study: HAp nanoparticles as direct cholecalciferol delivery agents and HAp nanoparticles coated with cholecalciferol-loaded poly(d,l)-lactide-co-glycolide (HAp/D3/PLGA).Satisfying biocompatibility of particulate systems, when incubated in contact with MC3T3-E1 osteoblastic cells in vitro, was observed for HAp/D3/PLGA and pure HAp. In contrast, an extensively fast release of cholecalciferol from the system comprising HAp nanoparticles coated with cholecalciferol (HAp/D3) triggered necrosis of the osteoblastic cells in vitro. Artificial defects induced in the osteoporotic bone of the rat mandible were successfully reconstructed following implantation of cholecalciferol-coated HAp nanoparticles as well as those comprising HAp nanoparticles coated with cholecalciferol-loaded PLGA (HAp/D3/PLGA). The greatest levels of enhanced angiogenesis, vascularization, osteogenesis and bone structure differentiation were achieved upon the implementation of HAp/D3/PLGA systems.
PB  - Elsevier
T2  - Materials Science and Engineering: C
T1  - Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol
SP  - 943
EP  - 950
DO  - 10.1016/j.msec.2012.11.026
ER  - 
@article{
author = "Ignjatović, Nenad L. and Uskoković, Vuk and Ajduković, Zorica and Uskoković, Dragan",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7411",
abstract = "Cholecalciferol, vitamin D3, plays an important role in bone metabolism by regulating extracellular levels of calcium. Presented here is a study on the effects of the local delivery of cholecalciferol (D3) using nanoparticulate carriers composed of hydroxyapatite (HAp) and poly(d,l-lactide-co-glycolide) (PLGA). Multifunctional nanoparticulate HAp-based powders were prepared for the purpose of: (a) either fast or sustained, local delivery of cholecalciferol, and (b) the secondary, osteoconductive and defect-filling effect of the carrier itself. Two types of HAp-based powders with particles of narrowly dispersed sizes in the nano range were prepared and tested in this study: HAp nanoparticles as direct cholecalciferol delivery agents and HAp nanoparticles coated with cholecalciferol-loaded poly(d,l)-lactide-co-glycolide (HAp/D3/PLGA).Satisfying biocompatibility of particulate systems, when incubated in contact with MC3T3-E1 osteoblastic cells in vitro, was observed for HAp/D3/PLGA and pure HAp. In contrast, an extensively fast release of cholecalciferol from the system comprising HAp nanoparticles coated with cholecalciferol (HAp/D3) triggered necrosis of the osteoblastic cells in vitro. Artificial defects induced in the osteoporotic bone of the rat mandible were successfully reconstructed following implantation of cholecalciferol-coated HAp nanoparticles as well as those comprising HAp nanoparticles coated with cholecalciferol-loaded PLGA (HAp/D3/PLGA). The greatest levels of enhanced angiogenesis, vascularization, osteogenesis and bone structure differentiation were achieved upon the implementation of HAp/D3/PLGA systems.",
publisher = "Elsevier",
journal = "Materials Science and Engineering: C",
title = "Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol",
pages = "943-950",
doi = "10.1016/j.msec.2012.11.026"
}
Ignjatović, N. L., Uskoković, V., Ajduković, Z.,& Uskoković, D. (2013). Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol.
Materials Science and Engineering: C
Elsevier., 943-950.
https://doi.org/10.1016/j.msec.2012.11.026
Ignjatović NL, Uskoković V, Ajduković Z, Uskoković D. Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol. Materials Science and Engineering: C. 2013;:943-950
Ignjatović Nenad L., Uskoković Vuk, Ajduković Zorica, Uskoković Dragan, "Multifunctional hydroxyapatite and poly(D,L-lactide-co-glycolide) nanoparticles for the local delivery of cholecalciferol" Materials Science and Engineering: C (2013):943-950,
https://doi.org/10.1016/j.msec.2012.11.026 .
41
37
43

Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones

Ignjatović, Nenad L.; Ajduković, Zorica; Savić, Vojin; Najman, Stevo; Mihailović, Dragan; Vasiljević, Perica; Stojanović, Zoran S.; Uskoković, Vuk; Uskoković, Dragan

(2013)

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Ajduković, Zorica
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Vasiljević, Perica
AU  - Stojanović, Zoran S.
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7410
AB  - Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.
T2  - Journal of Materials Science: Materials in Medicine
T1  - Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones
SP  - 343
EP  - 354
DO  - 10.1007/s10856-012-4793-1
ER  - 
@article{
author = "Ignjatović, Nenad L. and Ajduković, Zorica and Savić, Vojin and Najman, Stevo and Mihailović, Dragan and Vasiljević, Perica and Stojanović, Zoran S. and Uskoković, Vuk and Uskoković, Dragan",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7410",
abstract = "Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.",
journal = "Journal of Materials Science: Materials in Medicine",
title = "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones",
pages = "343-354",
doi = "10.1007/s10856-012-4793-1"
}
Ignjatović, N. L., Ajduković, Z., Savić, V., Najman, S., Mihailović, D., Vasiljević, P., Stojanović, Z. S., Uskoković, V.,& Uskoković, D. (2013). Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones.
Journal of Materials Science: Materials in Medicine, 343-354.
https://doi.org/10.1007/s10856-012-4793-1
Ignjatović NL, Ajduković Z, Savić V, Najman S, Mihailović D, Vasiljević P, Stojanović ZS, Uskoković V, Uskoković D. Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. Journal of Materials Science: Materials in Medicine. 2013;:343-354
Ignjatović Nenad L., Ajduković Zorica, Savić Vojin, Najman Stevo, Mihailović Dragan, Vasiljević Perica, Stojanović Zoran S., Uskoković Vuk, Uskoković Dragan, "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones" Journal of Materials Science: Materials in Medicine (2013):343-354,
https://doi.org/10.1007/s10856-012-4793-1 .
65
54
66

Biomaterials and their application in preprosthetic surgical procedure

Ajduković, Zorica; Đorđević, Nadica; Petrović, Nenad; Ignjatović, Nenad L.; Kenić Marinković, Dragana; Uskoković, Dragan

(Belgrade : Institute of Technical Sciences of SASA, 2013)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Đorđević, Nadica
AU  - Petrović, Nenad
AU  - Ignjatović, Nenad L.
AU  - Kenić Marinković, Dragana
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://dais.sanu.ac.rs/123456789/432
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/694
UR  - http://www.itn.sanu.ac.rs/opus4/files/694/Ajdukovic-12YRC-5.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7434
AB  - The loss of teeth and damage to the jaw bone occur under different circumstances. The deficit may be the result of wearing inadequate dentures, residual ridge resorption, osteoporosis, periodontal disease, trauma, tumors, radiation, etc. The deficit of bone tissue can be a substantial problem because it prevents adequate prosthetic patient care and restoration of disturbed functions of stomatognathic system. With such patients, there is often a need for preprosthetic surgical procedure through the application of various techniques and materials in order to resolve aftermentioned bone shortcomings. The most appropriate biological materials for the reconstruction are autogenous bone graft and artificial bone. With the development of technology and specificbiomaterials it is tried to avoid situations that require autogenous bone grafting, since it involvs long surgical procedure with the possibility of postoperative complications. The application of synthetic biomaterials, whose properties are similar to natural hydroxyapatite, promotes the biological potential for bone tissue repair. Synthetic biomaterials have great biocompatibility and sterility and do not act antigenicaly. The disadvantages of this material are overcome by the addition of organic polymers. Thanks to the stability and flexibility of hydroxyapatite structure, a large number of ionic changes are possible, both cationic and anionic, which improve the characteristics of synthetic hydroxyapatite. Biomaterials that include different drugs, such as antibiotics, vitamins and other preparations, could be used for the treatment of complications after surgical procedures and infection of bone tissue. Our experimental studies indicate that biomaterials based on hydroxyapatite are valuable materials that can be used for preprosthetic surgical preparation.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Twelfth Young Researchers' Conference Materials Sciences and Engineering December 11-13, 2013, Belgrade, Serbia
T1  - Biomaterials and their application in preprosthetic surgical procedure
SP  - 5
EP  - 5
ER  - 
@conference{
author = "Ajduković, Zorica and Đorđević, Nadica and Petrović, Nenad and Ignjatović, Nenad L. and Kenić Marinković, Dragana and Uskoković, Dragan",
year = "2013",
url = "http://dais.sanu.ac.rs/123456789/432, http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/694, http://www.itn.sanu.ac.rs/opus4/files/694/Ajdukovic-12YRC-5.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7434",
abstract = "The loss of teeth and damage to the jaw bone occur under different circumstances. The deficit may be the result of wearing inadequate dentures, residual ridge resorption, osteoporosis, periodontal disease, trauma, tumors, radiation, etc. The deficit of bone tissue can be a substantial problem because it prevents adequate prosthetic patient care and restoration of disturbed functions of stomatognathic system. With such patients, there is often a need for preprosthetic surgical procedure through the application of various techniques and materials in order to resolve aftermentioned bone shortcomings. The most appropriate biological materials for the reconstruction are autogenous bone graft and artificial bone. With the development of technology and specificbiomaterials it is tried to avoid situations that require autogenous bone grafting, since it involvs long surgical procedure with the possibility of postoperative complications. The application of synthetic biomaterials, whose properties are similar to natural hydroxyapatite, promotes the biological potential for bone tissue repair. Synthetic biomaterials have great biocompatibility and sterility and do not act antigenicaly. The disadvantages of this material are overcome by the addition of organic polymers. Thanks to the stability and flexibility of hydroxyapatite structure, a large number of ionic changes are possible, both cationic and anionic, which improve the characteristics of synthetic hydroxyapatite. Biomaterials that include different drugs, such as antibiotics, vitamins and other preparations, could be used for the treatment of complications after surgical procedures and infection of bone tissue. Our experimental studies indicate that biomaterials based on hydroxyapatite are valuable materials that can be used for preprosthetic surgical preparation.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Twelfth Young Researchers' Conference Materials Sciences and Engineering December 11-13, 2013, Belgrade, Serbia",
title = "Biomaterials and their application in preprosthetic surgical procedure",
pages = "5-5"
}
Ajduković, Z., Đorđević, N., Petrović, N., Ignjatović, N. L., Kenić Marinković, D.,& Uskoković, D. (2013). Biomaterials and their application in preprosthetic surgical procedure.
Program and the Book of Abstracts / Twelfth Young Researchers' Conference Materials Sciences and Engineering December 11-13, 2013, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 5-5.
Ajduković Z, Đorđević N, Petrović N, Ignjatović NL, Kenić Marinković D, Uskoković D. Biomaterials and their application in preprosthetic surgical procedure. Program and the Book of Abstracts / Twelfth Young Researchers' Conference Materials Sciences and Engineering December 11-13, 2013, Belgrade, Serbia. 2013;:5-5
Ajduković Zorica, Đorđević Nadica, Petrović Nenad, Ignjatović Nenad L., Kenić Marinković Dragana, Uskoković Dragan, "Biomaterials and their application in preprosthetic surgical procedure" Program and the Book of Abstracts / Twelfth Young Researchers' Conference Materials Sciences and Engineering December 11-13, 2013, Belgrade, Serbia (2013):5-5

Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta

Ajduković, Zorica; Ignjatović, Nenad L.; Savić, Vojin; Najman, Stevo; Mihailović, D.; Rajković, J.; Petrović, N.; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2012)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Mihailović, D.
AU  - Rajković, J.
AU  - Petrović, N.
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/455
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/717
UR  - http://www.itn.sanu.ac.rs/opus4/files/717/Ajdukovic-Yucomat2012-121b.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7444
AB  - In recent years, calcium hydroxyapatite (HAp) and its substituents are increasingly used in dentistry and medicine. The influence of nanoparticles Ca / Co-HAp in strengthening weak osteoporotic bone jaw tegmenta was tested in an experimental model. The study was conducted on Wistar soy rats, aged 6-8 weeks. The biomaterial was implanted in the osteoporoticly weakened mandible of these animals. The best results in the strengthening of the lower jaw bone tegmenta were achieved 24 weeks after implantation of hydroxyapatite nanoparticles in which the calcium ion was substituted with 12% of cobalt ions. Histochemical parameters of bone synthesis were in a statistically significant increase. SEM analysis showed a high degree of osteogenetic ability of nano-particulate material implanted in the bone defect.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1  - Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta
SP  - 121
EP  - 121
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad L. and Savić, Vojin and Najman, Stevo and Mihailović, D. and Rajković, J. and Petrović, N. and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/455, http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/717, http://www.itn.sanu.ac.rs/opus4/files/717/Ajdukovic-Yucomat2012-121b.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7444",
abstract = "In recent years, calcium hydroxyapatite (HAp) and its substituents are increasingly used in dentistry and medicine. The influence of nanoparticles Ca / Co-HAp in strengthening weak osteoporotic bone jaw tegmenta was tested in an experimental model. The study was conducted on Wistar soy rats, aged 6-8 weeks. The biomaterial was implanted in the osteoporoticly weakened mandible of these animals. The best results in the strengthening of the lower jaw bone tegmenta were achieved 24 weeks after implantation of hydroxyapatite nanoparticles in which the calcium ion was substituted with 12% of cobalt ions. Histochemical parameters of bone synthesis were in a statistically significant increase. SEM analysis showed a high degree of osteogenetic ability of nano-particulate material implanted in the bone defect.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta",
pages = "121-121"
}
Ajduković, Z., Ignjatović, N. L., Savić, V., Najman, S., Mihailović, D., Rajković, J., Petrović, N.,& Uskoković, D. (2012). Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta.
The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 121-121.
Ajduković Z, Ignjatović NL, Savić V, Najman S, Mihailović D, Rajković J, Petrović N, Uskoković D. Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta. The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:121-121
Ajduković Zorica, Ignjatović Nenad L., Savić Vojin, Najman Stevo, Mihailović D., Rajković J., Petrović N., Uskoković Dragan, "Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta" The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):121-121

Hemolytic activity of bioactive nanocomposites

Ajduković, Zorica; Ignjatović, Nenad L.; Petrović, N.; Najman, Stevo; Rajković, J.; Kenić Marinković, D.; Krstić, V.; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade, 2012)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Petrović, N.
AU  - Najman, Stevo
AU  - Rajković, J.
AU  - Kenić Marinković, D.
AU  - Krstić, V.
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/497
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/762
UR  - http://www.itn.sanu.ac.rs/opus4/files/762/Ajdukovic_11KMI.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7453
AB  - Huge range of tested biomaterials in recent decades has emerged as an ideal scaffold for cell growth, but few have demonstrated clinical efficacy. Among them, synthetic hydroxyapatite (HAp, Ca10(PO4)6(OH)2) is the most promising because of its biocompatibility, bioactivity, and osteoconductivity. Biocompatibility represents the primary concern for any material to be used as a substitute for natural tissue. Hydroxyapatite particles interact with numerous cellular systems in vivo, and some of these interactions may lead to cell damage and to stimulate platelet activation, coagulation and thrombus formation. The aim of this work was to examine the hemocompatibility of nanocalcium hydroxyapatite substituted with 5% and 12% cobalt (Ca /CoHAp) and hydroxyapatite/polylactidcoglicolid (HAp / PLGA) in relation to pure HAp by testing their hemolytic activities. The results show the discrepancy in hemolytic activity of implanted matherials. The degree of crystallinity of samples had a more dominant influence on hemolysis than the percentage of substituted cobalt. Hemolysis ratios of the nanocalcium hydroxyapatite substituted with cobalt samples were below 3%, indicating good blood compatibility and that they are promising for medical application.
PB  - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1  - Hemolytic activity of bioactive nanocomposites
SP  - 49
EP  - 49
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad L. and Petrović, N. and Najman, Stevo and Rajković, J. and Kenić Marinković, D. and Krstić, V. and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/497, http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/762, http://www.itn.sanu.ac.rs/opus4/files/762/Ajdukovic_11KMI.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7453",
abstract = "Huge range of tested biomaterials in recent decades has emerged as an ideal scaffold for cell growth, but few have demonstrated clinical efficacy. Among them, synthetic hydroxyapatite (HAp, Ca10(PO4)6(OH)2) is the most promising because of its biocompatibility, bioactivity, and osteoconductivity. Biocompatibility represents the primary concern for any material to be used as a substitute for natural tissue. Hydroxyapatite particles interact with numerous cellular systems in vivo, and some of these interactions may lead to cell damage and to stimulate platelet activation, coagulation and thrombus formation. The aim of this work was to examine the hemocompatibility of nanocalcium hydroxyapatite substituted with 5% and 12% cobalt (Ca /CoHAp) and hydroxyapatite/polylactidcoglicolid (HAp / PLGA) in relation to pure HAp by testing their hemolytic activities. The results show the discrepancy in hemolytic activity of implanted matherials. The degree of crystallinity of samples had a more dominant influence on hemolysis than the percentage of substituted cobalt. Hemolysis ratios of the nanocalcium hydroxyapatite substituted with cobalt samples were below 3%, indicating good blood compatibility and that they are promising for medical application.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Hemolytic activity of bioactive nanocomposites",
pages = "49-49"
}
Ajduković, Z., Ignjatović, N. L., Petrović, N., Najman, S., Rajković, J., Kenić Marinković, D., Krstić, V.,& Uskoković, D. (2012). Hemolytic activity of bioactive nanocomposites.
Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., 49-49.
Ajduković Z, Ignjatović NL, Petrović N, Najman S, Rajković J, Kenić Marinković D, Krstić V, Uskoković D. Hemolytic activity of bioactive nanocomposites. Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:49-49
Ajduković Zorica, Ignjatović Nenad L., Petrović N., Najman Stevo, Rajković J., Kenić Marinković D., Krstić V., Uskoković Dragan, "Hemolytic activity of bioactive nanocomposites" Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts (2012):49-49

Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges

Ajduković, Zorica; Ignjatović, Nenad L.; Rajković, Jelena; Najman, Stevo; Mihailović, D.; Petrović, N.; Kenić Marinković, D.; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade, 2012)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Rajković, Jelena
AU  - Najman, Stevo
AU  - Mihailović, D.
AU  - Petrović, N.
AU  - Kenić Marinković, D.
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/498
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/763
UR  - http://www.itn.sanu.ac.rs/opus4/files/763/Ajdukovic_11KMI-Y15.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7454
AB  - Bone loss during the systemic osteoporosis has an important role in dentistry and medicine. The aim of the study was the application of bioactive micro and nanocomposite materials, alone, and in combination with autologous plasma in osteoporotic jaw bones of rats with artificially induced osteoporosis. The effect of these composites was measured by histomorphometric and atomic absorption spectrophotometric analysis. According to the best obtained results in regeneration and recovery of the resorbed alveolar bone, it can be concluded that nanocomposite combined with autologous plasma may be the material of choice to replace the osteoporotic damaged jaw bone.
PB  - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1  - Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges
SP  - 103
EP  - 103
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad L. and Rajković, Jelena and Najman, Stevo and Mihailović, D. and Petrović, N. and Kenić Marinković, D. and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/498, http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/763, http://www.itn.sanu.ac.rs/opus4/files/763/Ajdukovic_11KMI-Y15.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7454",
abstract = "Bone loss during the systemic osteoporosis has an important role in dentistry and medicine. The aim of the study was the application of bioactive micro and nanocomposite materials, alone, and in combination with autologous plasma in osteoporotic jaw bones of rats with artificially induced osteoporosis. The effect of these composites was measured by histomorphometric and atomic absorption spectrophotometric analysis. According to the best obtained results in regeneration and recovery of the resorbed alveolar bone, it can be concluded that nanocomposite combined with autologous plasma may be the material of choice to replace the osteoporotic damaged jaw bone.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges",
pages = "103-103"
}
Ajduković, Z., Ignjatović, N. L., Rajković, J., Najman, S., Mihailović, D., Petrović, N., Kenić Marinković, D.,& Uskoković, D. (2012). Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges.
Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., 103-103.
Ajduković Z, Ignjatović NL, Rajković J, Najman S, Mihailović D, Petrović N, Kenić Marinković D, Uskoković D. Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges. Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:103-103
Ajduković Zorica, Ignjatović Nenad L., Rajković Jelena, Najman Stevo, Mihailović D., Petrović N., Kenić Marinković D., Uskoković Dragan, "Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges" Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts (2012):103-103

Regeneration bone tissue by new nanoparticules system based on hydroxiapatite as systems for local delivery of vitamin D3

Ajduković, Zorica; Petrović, M.; Ignjatović, Nenad L.; Savić, Vojin; Mihailović, D.; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2012)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Petrović, M.
AU  - Ignjatović, Nenad L.
AU  - Savić, Vojin
AU  - Mihailović, D.
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/716
UR  - http://dais.sanu.ac.rs/123456789/454
UR  - http://www.itn.sanu.ac.rs/opus4/files/716/Ajdukovic-Yucomat2012-121a.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7443
AB  - Increased life expectancy in developed countries leads to an increase in the number of musculoskeletal disorders, such as osteoporosis, oasteoratritis thus compromising good dental treatment. There are many drug delivery systems based on hydroxyapatite used in bone tissue regeneration. Vitamin D3 is osteodiferentiation factor which regulates bone formation by increasing osteoblast differentiation and bone mineralization. The aim of this study is to examine new multifunctional nanoparticulate system for local delivery of active form of vitamin D3 by biochemical blood marker and histochemical analysis. The research was carried out on female Westar rats, aged 6-8 weeks, which have been implanted biomaterials in the artificial bone defect. Biochemical markers of osteogenesis were statistically significant after only 6 weeks of implantation. ALP activity in bone tissue was showed by histochemical analysis as well as high level reparatory skills. Local realized Vitamin D3 contribute to bone formation by increasing osteoblast differentiation and bone mineralization.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1  - Regeneration bone tissue by new nanoparticules system based on hydroxiapatite as systems for local delivery of vitamin D3
SP  - 121
EP  - 121
ER  - 
@conference{
author = "Ajduković, Zorica and Petrović, M. and Ignjatović, Nenad L. and Savić, Vojin and Mihailović, D. and Uskoković, Dragan",
year = "2012",
url = "http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/716, http://dais.sanu.ac.rs/123456789/454, http://www.itn.sanu.ac.rs/opus4/files/716/Ajdukovic-Yucomat2012-121a.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7443",
abstract = "Increased life expectancy in developed countries leads to an increase in the number of musculoskeletal disorders, such as osteoporosis, oasteoratritis thus compromising good dental treatment. There are many drug delivery systems based on hydroxyapatite used in bone tissue regeneration. Vitamin D3 is osteodiferentiation factor which regulates bone formation by increasing osteoblast differentiation and bone mineralization. The aim of this study is to examine new multifunctional nanoparticulate system for local delivery of active form of vitamin D3 by biochemical blood marker and histochemical analysis. The research was carried out on female Westar rats, aged 6-8 weeks, which have been implanted biomaterials in the artificial bone defect. Biochemical markers of osteogenesis were statistically significant after only 6 weeks of implantation. ALP activity in bone tissue was showed by histochemical analysis as well as high level reparatory skills. Local realized Vitamin D3 contribute to bone formation by increasing osteoblast differentiation and bone mineralization.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Regeneration bone tissue by new nanoparticules system based on hydroxiapatite as systems for local delivery of vitamin D3",
pages = "121-121"
}
Ajduković, Z., Petrović, M., Ignjatović, N. L., Savić, V., Mihailović, D.,& Uskoković, D. (2012). Regeneration bone tissue by new nanoparticules system based on hydroxiapatite as systems for local delivery of vitamin D3.
The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 121-121.
Ajduković Z, Petrović M, Ignjatović NL, Savić V, Mihailović D, Uskoković D. Regeneration bone tissue by new nanoparticules system based on hydroxiapatite as systems for local delivery of vitamin D3. The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:121-121
Ajduković Zorica, Petrović M., Ignjatović Nenad L., Savić Vojin, Mihailović D., Uskoković Dragan, "Regeneration bone tissue by new nanoparticules system based on hydroxiapatite as systems for local delivery of vitamin D3" The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):121-121

Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells

Stojanović, S.; Najman, Stevo; Ajduković, Zorica; Ignjatović, Nenad L.; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2012)

TY  - CONF
AU  - Stojanović, S.
AU  - Najman, Stevo
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/720
UR  - http://dais.sanu.ac.rs/123456789/458
UR  - http://www.itn.sanu.ac.rs/opus4/files/720/Stojanovic-Yucomat2012-126.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7445
AB  - The aim of this study was to compare the effects of two types of nanomaterials, cobalt-exchanged hydroxyapatite (CoHAp) and calcium phosphate/poly-(DL-lactide-co-glycolide) (CP/PLGA), on the viability of Saos-2 osteoblast-like cells, using MTT test. We have examined the suspension of this materials in the following concentrations: 1.6, 8, 40, 200, 1000 and 2500 μg/ml, as well as extracts in concentration range from 2,5% to 100%. Both materials showed cytotoxic effect at higher concentrations of suspension and extract, respectively, but they were not cytotoxic at lower concentrations. CP/PLGA acted stronger cytotoxic compared to CoHAp, regardless of whether it is examined suspension or extract. CoHAp in small concentrations of suspension and extract acted slightly stimulatory on cells. This suggests that CoHAp may have advantage for use in the in vivo systems.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1  - Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells
SP  - 126
EP  - 126
ER  - 
@conference{
author = "Stojanović, S. and Najman, Stevo and Ajduković, Zorica and Ignjatović, Nenad L. and Uskoković, Dragan",
year = "2012",
url = "http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/720, http://dais.sanu.ac.rs/123456789/458, http://www.itn.sanu.ac.rs/opus4/files/720/Stojanovic-Yucomat2012-126.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7445",
abstract = "The aim of this study was to compare the effects of two types of nanomaterials, cobalt-exchanged hydroxyapatite (CoHAp) and calcium phosphate/poly-(DL-lactide-co-glycolide) (CP/PLGA), on the viability of Saos-2 osteoblast-like cells, using MTT test. We have examined the suspension of this materials in the following concentrations: 1.6, 8, 40, 200, 1000 and 2500 μg/ml, as well as extracts in concentration range from 2,5% to 100%. Both materials showed cytotoxic effect at higher concentrations of suspension and extract, respectively, but they were not cytotoxic at lower concentrations. CP/PLGA acted stronger cytotoxic compared to CoHAp, regardless of whether it is examined suspension or extract. CoHAp in small concentrations of suspension and extract acted slightly stimulatory on cells. This suggests that CoHAp may have advantage for use in the in vivo systems.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells",
pages = "126-126"
}
Stojanović, S., Najman, S., Ajduković, Z., Ignjatović, N. L.,& Uskoković, D. (2012). Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells.
The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 126-126.
Stojanović S, Najman S, Ajduković Z, Ignjatović NL, Uskoković D. Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells. The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:126-126
Stojanović S., Najman Stevo, Ajduković Zorica, Ignjatović Nenad L., Uskoković Dragan, "Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells" The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):126-126

Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells

Najman, Stevo; Stojanović, Sanja; Mitrić, Žarko; Ajduković, Zorica; Ignjatović, Nenad L.; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade, 2012)

TY  - CONF
AU  - Najman, Stevo
AU  - Stojanović, Sanja
AU  - Mitrić, Žarko
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/519
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/786
UR  - http://www.itn.sanu.ac.rs/opus4/files/786/Najman_11KMI.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7466
AB  - We have examined the effect of extracts of the calcium phosphate/poly(DL-lactide-co-glycolide) (CP/PLGA) and cobalt-exchanged hydroxyapatite (CoHAp), on the viability of Saos2 osteoblastlike cells. Extracts were prepared by incubation for 3 days at 37°C in the cultivation medium. Conductivity and pH value of extracts were measured before viability assay. Cell viability was estimated by MTT test performed after 24 h incubation of cells with various concentrations of extracts. Extract of CP/PLGA acted more cytotoxic on osteoblasts than the extract of CoHAp. This difference in the effect of examined extracts can be explained by their different pH value and conductivity.
PB  - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1  - Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells
SP  - 110
EP  - 110
ER  - 
@conference{
author = "Najman, Stevo and Stojanović, Sanja and Mitrić, Žarko and Ajduković, Zorica and Ignjatović, Nenad L. and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/519, http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/786, http://www.itn.sanu.ac.rs/opus4/files/786/Najman_11KMI.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7466",
abstract = "We have examined the effect of extracts of the calcium phosphate/poly(DL-lactide-co-glycolide) (CP/PLGA) and cobalt-exchanged hydroxyapatite (CoHAp), on the viability of Saos2 osteoblastlike cells. Extracts were prepared by incubation for 3 days at 37°C in the cultivation medium. Conductivity and pH value of extracts were measured before viability assay. Cell viability was estimated by MTT test performed after 24 h incubation of cells with various concentrations of extracts. Extract of CP/PLGA acted more cytotoxic on osteoblasts than the extract of CoHAp. This difference in the effect of examined extracts can be explained by their different pH value and conductivity.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells",
pages = "110-110"
}
Najman, S., Stojanović, S., Mitrić, Ž., Ajduković, Z., Ignjatović, N. L.,& Uskoković, D. (2012). Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells.
Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., 110-110.
Najman S, Stojanović S, Mitrić Ž, Ajduković Z, Ignjatović NL, Uskoković D. Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells. Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:110-110
Najman Stevo, Stojanović Sanja, Mitrić Žarko, Ajduković Zorica, Ignjatović Nenad L., Uskoković Dragan, "Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells" Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts (2012):110-110

Multifunctional nano scale drug delivery particles based on vitamin D3-loaded hydroxyapatite in bone tissue engineering

Ignjatović, Nenad L.; Ajduković, Zorica; Uskoković, Vuk; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2012)

TY  - CONF
AU  - Ignjatović, Nenad L.
AU  - Ajduković, Zorica
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/696
UR  - http://dais.sanu.ac.rs/123456789/434
UR  - http://www.itn.sanu.ac.rs/opus4/files/696/Ignjatovic-Yucomat2012-32.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7436
AB  - Specific group of controlled drug delivery systems in bone tissue engineering are multifunctional nanoparticle systems (MNPs) based on hydroxyapatite coated with drug loadedbioresorbable polymer. This study illustrates the possibility for controlled synthesis of multifunctional nanoparticulate forms based on hydroxyapatite as a system for local delivery of vitamin D3 and secondary delivery of defect filler hydroxyapatite. The results were two type of nanoparticle powder of controlled shapes, sizes and properties: hydroxyapatite nano particles as vitamin D3 carriers (HAp/D3) and vitamin D3-loaded hydroxyapatite coated with poly-D,Llactide-co-glycolide (HAp/D3/PLGA) with particle sizes of d50=68 nm and d50=71 nm, respectively. Simultaneously, biocompatibility of the materials was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. Biocompatibility tests carried out on cell cultures have shown intact monolayers of epithelial cells in contact with both materials and no negative effects on the cell viability. The aim of this work was application in living/human systems, and it has been tested in vivo; artificially made bone defects of a mandible damaged by osteoporosis have been reconstructed with both types of materials. The best results were achieved 24 weeks after implantation of vitamin D3-loaded hydroxyapatite coated with poly-D,L-lactide-co-glycolide. Accelerated angiogenesis, vascularization, osteogenesis and bone structure differentiation has been achieved in the presence of specific islet-like forms of ossification centers.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1  - Multifunctional nano scale drug delivery particles based on vitamin D3-loaded hydroxyapatite in bone tissue engineering
SP  - 32
EP  - 32
ER  - 
@conference{
author = "Ignjatović, Nenad L. and Ajduković, Zorica and Uskoković, Vuk and Uskoković, Dragan",
year = "2012",
url = "http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/696, http://dais.sanu.ac.rs/123456789/434, http://www.itn.sanu.ac.rs/opus4/files/696/Ignjatovic-Yucomat2012-32.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7436",
abstract = "Specific group of controlled drug delivery systems in bone tissue engineering are multifunctional nanoparticle systems (MNPs) based on hydroxyapatite coated with drug loadedbioresorbable polymer. This study illustrates the possibility for controlled synthesis of multifunctional nanoparticulate forms based on hydroxyapatite as a system for local delivery of vitamin D3 and secondary delivery of defect filler hydroxyapatite. The results were two type of nanoparticle powder of controlled shapes, sizes and properties: hydroxyapatite nano particles as vitamin D3 carriers (HAp/D3) and vitamin D3-loaded hydroxyapatite coated with poly-D,Llactide-co-glycolide (HAp/D3/PLGA) with particle sizes of d50=68 nm and d50=71 nm, respectively. Simultaneously, biocompatibility of the materials was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. Biocompatibility tests carried out on cell cultures have shown intact monolayers of epithelial cells in contact with both materials and no negative effects on the cell viability. The aim of this work was application in living/human systems, and it has been tested in vivo; artificially made bone defects of a mandible damaged by osteoporosis have been reconstructed with both types of materials. The best results were achieved 24 weeks after implantation of vitamin D3-loaded hydroxyapatite coated with poly-D,L-lactide-co-glycolide. Accelerated angiogenesis, vascularization, osteogenesis and bone structure differentiation has been achieved in the presence of specific islet-like forms of ossification centers.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Multifunctional nano scale drug delivery particles based on vitamin D3-loaded hydroxyapatite in bone tissue engineering",
pages = "32-32"
}
Ignjatović, N. L., Ajduković, Z., Uskoković, V.,& Uskoković, D. (2012). Multifunctional nano scale drug delivery particles based on vitamin D3-loaded hydroxyapatite in bone tissue engineering.
The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 32-32.
Ignjatović NL, Ajduković Z, Uskoković V, Uskoković D. Multifunctional nano scale drug delivery particles based on vitamin D3-loaded hydroxyapatite in bone tissue engineering. The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:32-32
Ignjatović Nenad L., Ajduković Zorica, Uskoković Vuk, Uskoković Dragan, "Multifunctional nano scale drug delivery particles based on vitamin D3-loaded hydroxyapatite in bone tissue engineering" The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):32-32

Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density

Ajduković, Zorica; Petrović, Milica B.; Milićević, J.; Đorđević, N.; Ignjatović, Nenad L.; Savić, Vojin; Najman, Stevo; Mihailović, Dragan; Vasiljević, P.; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2011)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Petrović, Milica B.
AU  - Milićević, J.
AU  - Đorđević, N.
AU  - Ignjatović, Nenad L.
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Vasiljević, P.
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/945
UR  - http://dais.sanu.ac.rs/123456789/674
UR  - http://www.itn.sanu.ac.rs/opus4/files/945/Ajdukovic_YUCOMAT2011.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7505
AB  - One of the trends in the treatment of reduced bone density is in oral implantology through the use of new biomaterials with paramagnetic properties. In this in vivo study the effect of Ca /Co-HAp nanoparticles (calcium/cobalt-hydroxiapatite) in the regeneration of the reduced alveolar bone density in experimental animals was examined by analyzing both the biochemical and histochemical markers and through analysis of alveolar bone density. The research was carried out on female Westar rats, aged 6-8 weeks, whom have been implanted nanobiomaterial in reduced alveolar bone density. Good results in regeneration of reduced bone density were achieved in six weeks after implantation of the nanoparticles with paramagnetic properties. Biochemical markers of osteogenesis showed statistically significant rise. Histological analysis revealed high level reparatory skills of the biocomposite implanted in the bone defect, while X-ray analyses evidenced rise of bone density. Implantation of Ca/Co-Hap enables rapid new formation, thus becoming the material of choice for accelerated reduced bone density regeneration.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts
T1  - Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density
SP  - 161
EP  - 161
ER  - 
@conference{
author = "Ajduković, Zorica and Petrović, Milica B. and Milićević, J. and Đorđević, N. and Ignjatović, Nenad L. and Savić, Vojin and Najman, Stevo and Mihailović, Dragan and Vasiljević, P. and Uskoković, Dragan",
year = "2011",
url = "http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/945, http://dais.sanu.ac.rs/123456789/674, http://www.itn.sanu.ac.rs/opus4/files/945/Ajdukovic_YUCOMAT2011.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7505",
abstract = "One of the trends in the treatment of reduced bone density is in oral implantology through the use of new biomaterials with paramagnetic properties. In this in vivo study the effect of Ca /Co-HAp nanoparticles (calcium/cobalt-hydroxiapatite) in the regeneration of the reduced alveolar bone density in experimental animals was examined by analyzing both the biochemical and histochemical markers and through analysis of alveolar bone density. The research was carried out on female Westar rats, aged 6-8 weeks, whom have been implanted nanobiomaterial in reduced alveolar bone density. Good results in regeneration of reduced bone density were achieved in six weeks after implantation of the nanoparticles with paramagnetic properties. Biochemical markers of osteogenesis showed statistically significant rise. Histological analysis revealed high level reparatory skills of the biocomposite implanted in the bone defect, while X-ray analyses evidenced rise of bone density. Implantation of Ca/Co-Hap enables rapid new formation, thus becoming the material of choice for accelerated reduced bone density regeneration.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts",
title = "Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density",
pages = "161-161"
}
Ajduković, Z., Petrović, M. B., Milićević, J., Đorđević, N., Ignjatović, N. L., Savić, V., Najman, S., Mihailović, D., Vasiljević, P.,& Uskoković, D. (2011). Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density.
The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 161-161.
Ajduković Z, Petrović MB, Milićević J, Đorđević N, Ignjatović NL, Savić V, Najman S, Mihailović D, Vasiljević P, Uskoković D. Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density. The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts. 2011;:161-161
Ajduković Zorica, Petrović Milica B., Milićević J., Đorđević N., Ignjatović Nenad L., Savić Vojin, Najman Stevo, Mihailović Dragan, Vasiljević P., Uskoković Dragan, "Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density" The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts (2011):161-161

Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration

Ajduković, Zorica; Ignjatović, Nenad L.; Petrović, Nenad; Savić, Vojin; Mihailović, Dragan; Uskoković, Dragan

(Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, 2011)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Petrović, Nenad
AU  - Savić, Vojin
AU  - Mihailović, Dragan
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/690
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/962
UR  - http://www.itn.sanu.ac.rs/opus4/files/962/Ajdukovic_10KMI.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7512
AB  - Systems for targeted delivery of drugs with high affinity for specific organs, tissues, and cells were introduced at the beginning of the twentieth century. Recently, this concept is attracting much attention. These systems have shown most effective in reducing adverse effects of drugs. In recent years, scientists have created a system of nanoparticles for delivery of vitamin D. Special attention is paid to the role of vitamin D in the division and differentiation of osteoblasts and thus, in its role in osteogenesis. In this regard it was interesting to observe the local effects of the active form of vitamin D3 in osteogenesis, as a part of the system for local delivery. These effects were observed on an experimental model. Three types of nanobiomaterial were implanted in the artificially made defects in rats’ mandibular bone. Six weeks after implantation of hydroxyapatite, hydroxyapatite-coated vitamin D3 and hydroxyapatite nanoparticles coated with vitamin D3 mixed with the polymer, the animals were sacrificed, and the samples of mandibular alveolar bone were taken. The samples were adequately prepared and subjected to histopathological analysis. The best results in regeneration of osteoporotic jaw bone were obtained in the experimental group of animals where the implant was hydroxyapatite-coated vitamin D3 mixed with the polymer. The results show that nanobiomaterials with local delivery of active form of vitamin D3, used in this study, may be materials of the future in the area of weakened jaw bone tissue regeneration.
PB  - Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts
C3  - Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia
T1  - Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration
SP  - 9
EP  - 9
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad L. and Petrović, Nenad and Savić, Vojin and Mihailović, Dragan and Uskoković, Dragan",
year = "2011",
url = "http://dais.sanu.ac.rs/123456789/690, http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/962, http://www.itn.sanu.ac.rs/opus4/files/962/Ajdukovic_10KMI.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7512",
abstract = "Systems for targeted delivery of drugs with high affinity for specific organs, tissues, and cells were introduced at the beginning of the twentieth century. Recently, this concept is attracting much attention. These systems have shown most effective in reducing adverse effects of drugs. In recent years, scientists have created a system of nanoparticles for delivery of vitamin D. Special attention is paid to the role of vitamin D in the division and differentiation of osteoblasts and thus, in its role in osteogenesis. In this regard it was interesting to observe the local effects of the active form of vitamin D3 in osteogenesis, as a part of the system for local delivery. These effects were observed on an experimental model. Three types of nanobiomaterial were implanted in the artificially made defects in rats’ mandibular bone. Six weeks after implantation of hydroxyapatite, hydroxyapatite-coated vitamin D3 and hydroxyapatite nanoparticles coated with vitamin D3 mixed with the polymer, the animals were sacrificed, and the samples of mandibular alveolar bone were taken. The samples were adequately prepared and subjected to histopathological analysis. The best results in regeneration of osteoporotic jaw bone were obtained in the experimental group of animals where the implant was hydroxyapatite-coated vitamin D3 mixed with the polymer. The results show that nanobiomaterials with local delivery of active form of vitamin D3, used in this study, may be materials of the future in the area of weakened jaw bone tissue regeneration.",
publisher = "Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts",
journal = "Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia",
title = "Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration",
pages = "9-9"
}
Ajduković, Z., Ignjatović, N. L., Petrović, N., Savić, V., Mihailović, D.,& Uskoković, D. (2011). Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration.
Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts., 9-9.
Ajduković Z, Ignjatović NL, Petrović N, Savić V, Mihailović D, Uskoković D. Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration. Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia. 2011;:9-9
Ajduković Zorica, Ignjatović Nenad L., Petrović Nenad, Savić Vojin, Mihailović Dragan, Uskoković Dragan, "Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration" Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia (2011):9-9

Multifunctional nano particulate systems based on hydroxyapatite as systems for local delivery of vitamin D3

Ignjatović, Nenad L.; Ajduković, Zorica; Drmanić, S.; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2011)

TY  - CONF
AU  - Ignjatović, Nenad L.
AU  - Ajduković, Zorica
AU  - Drmanić, S.
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/951
UR  - http://dais.sanu.ac.rs/123456789/679
UR  - http://www.itn.sanu.ac.rs/opus4/files/951/Ignjatovic_YUCOMAT2011.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7507
AB  - Multifunctional drug delivery system based on hydroxyapatite may be a research challenge in the treatment and reconstruction of bone tissue. Vitamin D3 has a positive effect on osteogenesis as it increases osteoblast differentiation and mineralization of bone tissue. The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new multifunctional nanoparticulate system for local delivery of vitamin D3 suitable for applications in bone engineering. The synthesis, characterization and application of three nano particle systems was shown: hydroxyapatite, hydroxyapatite/vitamin D3 and hydroxyapatite/PLGA/vitamin D3. The obtained vitamin D3-loaded nanoparticles based on hydroxyapatite. Characterization was performed with wide-angle X-ray structural analysis (XRD), infrared spectroscopy (FT-IR), field-emission scanning electron microscopy (FESEM), zeta potential (ZP) and particle sized distribution (PSD) methods. Basic in vitro studies were performed implantation of materials in rats. XRD and FT-IR analyses confirmed that the vitamin D3 is loaded hydroxyapatite and PLGA. Different values of zeta potential may refer to different phenomenological processes during in vivo studies.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts
T1  - Multifunctional nano particulate systems based on hydroxyapatite as systems for local delivery of vitamin D3
SP  - 68
EP  - 68
ER  - 
@conference{
author = "Ignjatović, Nenad L. and Ajduković, Zorica and Drmanić, S. and Uskoković, Dragan",
year = "2011",
url = "http://www.itn.sanu.ac.rs/opus4/frontdoor/index/index/docId/951, http://dais.sanu.ac.rs/123456789/679, http://www.itn.sanu.ac.rs/opus4/files/951/Ignjatovic_YUCOMAT2011.pdf, http://vinar.vin.bg.ac.rs/handle/123456789/7507",
abstract = "Multifunctional drug delivery system based on hydroxyapatite may be a research challenge in the treatment and reconstruction of bone tissue. Vitamin D3 has a positive effect on osteogenesis as it increases osteoblast differentiation and mineralization of bone tissue. The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new multifunctional nanoparticulate system for local delivery of vitamin D3 suitable for applications in bone engineering. The synthesis, characterization and application of three nano particle systems was shown: hydroxyapatite, hydroxyapatite/vitamin D3 and hydroxyapatite/PLGA/vitamin D3. The obtained vitamin D3-loaded nanoparticles based on hydroxyapatite. Characterization was performed with wide-angle X-ray structural analysis (XRD), infrared spectroscopy (FT-IR), field-emission scanning electron microscopy (FESEM), zeta potential (ZP) and particle sized distribution (PSD) methods. Basic in vitro studies were performed implantation of materials in rats. XRD and FT-IR analyses confirmed that the vitamin D3 is loaded hydroxyapatite and PLGA. Different values of zeta potential may refer to different phenomenological processes during in vivo studies.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts",
title = "Multifunctional nano particulate systems based on hydroxyapatite as systems for local delivery of vitamin D3",
pages = "68-68"
}
Ignjatović, N. L., Ajduković, Z., Drmanić, S.,& Uskoković, D. (2011). Multifunctional nano particulate systems based on hydroxyapatite as systems for local delivery of vitamin D3.
The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 68-68.
Ignjatović NL, Ajduković Z, Drmanić S, Uskoković D. Multifunctional nano particulate systems based on hydroxyapatite as systems for local delivery of vitamin D3. The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts. 2011;:68-68
Ignjatović Nenad L., Ajduković Zorica, Drmanić S., Uskoković Dragan, "Multifunctional nano particulate systems based on hydroxyapatite as systems for local delivery of vitamin D3" The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts (2011):68-68