TY  - CONF
AU  - Žakula, Jelena
AU  - Popović, Nataša
AU  - Mićević, Mirjana
AU  - Aškrabić, Sonja
AU  - Stojadinović, Bojan
AU  - Korićanac, Lela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12640
AB  - Background: Cancer nanomedicine is a rapidly developing fi eld that uses nanoparƟ cles (NPs) for the diagnosis and treatment of cancer. Currently, many nanomaterials with diff erent shapes, sizes, structures, and composiƟ ons have been invesƟ gated to produce eff ecƟ ve anƟ cancer NPs. The interest in the biomedical applicaƟ ons of bismuth-containing nanoparƟ cles, such as bismuth ferrite (BFO-NP) is a result of their promising properƟ es such as cost-effecƟ veness, chemical inertness, high stability, and simplicity of funcƟ onalizaƟ on. Material and Methods: A375 human melanoma and HeLa cervical carcinoma cells were used to study the anƟ tumor acƟ vity of BFO-NP. Clonogenicity of treated cells was analyzed by colony forming assay, while cell death was examined using fl ow cytometry. DCF-DA fl uorescent assay was applied to measure ROS producƟ on. Protein expression of p62 and TfR1 was detected by Western blot. Cell migraƟ on was analyzed using a wound scratch assay, while an SRB assay was used to assess cell adhesion. Results: BFO-NP (200 ng/L) signifi cantly reduced the clonogenicity of A375 and HeLa cells by 46 and 60%, respecƟ vely. Detected ROS producƟ on was increased considerably, especially for A375 melanoma cells, and amounted to 400%. The number of late apoptoƟ c and/or necroƟ c cells increased by 10-12%, compared to the control. Signifi cantly increased expression of autophagy-related protein p62 was observed in both cell lines aŌ er BFO-NP treatment. Ferroptosis-related transferrin receptor (TfR1) expression was slightly increased in treated A375 end HeLa cells (~14%). The noƟ ced increase in cell adhesion ranged from 20-30% followed by a decrease in cell migraƟ on. Conclusion:BFO-NP is a promising anƟ tumor agent with a signifi cant inhibitory eff ect on A375 and HeLa cell growth and metastaƟ c potenƟ al. Molecular mechanisms involved in these processes include ROS producƟ on and increased p62 expression. Reduced metastaƟ c potenƟ al resulted from the inducƟ onof cell adhesion and decreased cell migraƟ on.
PB  - Belgrade : Serbian Association for Cancer Research
C3  - Oncology Insights
T1  - Bismuth ferrite nanoparticles increase ROS production and p62 expression in A375 melanoma and HeLa cells
IS  - 1
SP  - 100
EP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12640
ER  - 

@conference{
author = "Žakula, Jelena and Popović, Nataša and Mićević, Mirjana and Aškrabić, Sonja and Stojadinović, Bojan and Korićanac, Lela",
year = "2023",
abstract = "Background: Cancer nanomedicine is a rapidly developing fi eld that uses nanoparƟ cles (NPs) for the diagnosis and treatment of cancer. Currently, many nanomaterials with diff erent shapes, sizes, structures, and composiƟ ons have been invesƟ gated to produce eff ecƟ ve anƟ cancer NPs. The interest in the biomedical applicaƟ ons of bismuth-containing nanoparƟ cles, such as bismuth ferrite (BFO-NP) is a result of their promising properƟ es such as cost-effecƟ veness, chemical inertness, high stability, and simplicity of funcƟ onalizaƟ on. Material and Methods: A375 human melanoma and HeLa cervical carcinoma cells were used to study the anƟ tumor acƟ vity of BFO-NP. Clonogenicity of treated cells was analyzed by colony forming assay, while cell death was examined using fl ow cytometry. DCF-DA fl uorescent assay was applied to measure ROS producƟ on. Protein expression of p62 and TfR1 was detected by Western blot. Cell migraƟ on was analyzed using a wound scratch assay, while an SRB assay was used to assess cell adhesion. Results: BFO-NP (200 ng/L) signifi cantly reduced the clonogenicity of A375 and HeLa cells by 46 and 60%, respecƟ vely. Detected ROS producƟ on was increased considerably, especially for A375 melanoma cells, and amounted to 400%. The number of late apoptoƟ c and/or necroƟ c cells increased by 10-12%, compared to the control. Signifi cantly increased expression of autophagy-related protein p62 was observed in both cell lines aŌ er BFO-NP treatment. Ferroptosis-related transferrin receptor (TfR1) expression was slightly increased in treated A375 end HeLa cells (~14%). The noƟ ced increase in cell adhesion ranged from 20-30% followed by a decrease in cell migraƟ on. Conclusion:BFO-NP is a promising anƟ tumor agent with a signifi cant inhibitory eff ect on A375 and HeLa cell growth and metastaƟ c potenƟ al. Molecular mechanisms involved in these processes include ROS producƟ on and increased p62 expression. Reduced metastaƟ c potenƟ al resulted from the inducƟ onof cell adhesion and decreased cell migraƟ on.",
publisher = "Belgrade : Serbian Association for Cancer Research",
journal = "Oncology Insights",
title = "Bismuth ferrite nanoparticles increase ROS production and p62 expression in A375 melanoma and HeLa cells",
number = "1",
pages = "100-101",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12640"
}

Žakula, J., Popović, N., Mićević, M., Aškrabić, S., Stojadinović, B.,& Korićanac, L.. (2023). Bismuth ferrite nanoparticles increase ROS production and p62 expression in A375 melanoma and HeLa cells. in Oncology Insights
Belgrade : Serbian Association for Cancer Research.(1), 100-101.
https://hdl.handle.net/21.15107/rcub_vinar_12640

Žakula J, Popović N, Mićević M, Aškrabić S, Stojadinović B, Korićanac L. Bismuth ferrite nanoparticles increase ROS production and p62 expression in A375 melanoma and HeLa cells. in Oncology Insights. 2023;(1):100-101.
https://hdl.handle.net/21.15107/rcub_vinar_12640 .

Žakula, Jelena, Popović, Nataša, Mićević, Mirjana, Aškrabić, Sonja, Stojadinović, Bojan, Korićanac, Lela, "Bismuth ferrite nanoparticles increase ROS production and p62 expression in A375 melanoma and HeLa cells" in Oncology Insights, no. 1 (2023):100-101,
https://hdl.handle.net/21.15107/rcub_vinar_12640 .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Vujović, Predrag
AU  - Pajović, Snežana B.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10235
AB  - Background This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS). Methods The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities. Results We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals. Conclusions These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understanding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress.
T2  - PeerJ
T1  - Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium
VL  - 10
SP  - e13020
DO  - 10.7717/peerj.13020
ER  - 

@article{
author = "Gavrilović, Ljubica and Popović, Nataša and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Vujović, Predrag and Pajović, Snežana B.",
year = "2022",
abstract = "Background This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS). Methods The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities. Results We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals. Conclusions These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understanding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress.",
journal = "PeerJ",
title = "Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium",
volume = "10",
pages = "e13020",
doi = "10.7717/peerj.13020"
}

Gavrilović, L., Popović, N., Stojiljković, V., Pejić, S., Todorović, A., Vujović, P.,& Pajović, S. B.. (2022). Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium. in PeerJ, 10, e13020.
https://doi.org/10.7717/peerj.13020

Gavrilović L, Popović N, Stojiljković V, Pejić S, Todorović A, Vujović P, Pajović SB. Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium. in PeerJ. 2022;10:e13020.
doi:10.7717/peerj.13020 .

Gavrilović, Ljubica, Popović, Nataša, Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Vujović, Predrag, Pajović, Snežana B., "Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium" in PeerJ, 10 (2022):e13020,
https://doi.org/10.7717/peerj.13020 . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Pantelić, Marija
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9959
AB  - Data about the dynamics of noradrenaline (NA) transmission, storage and degradation may be very important for understanding the reduction of functional deficiency of NA and maintaining the stability of NA levels in animals with depressive-like behavior treated with lithium. This study aimed to investigate the effects of mood stabilizer lithium on concentrations of NA in the prefrontal cortex (PFC), as well as behavior rats exposed to chronic restraint stress (CRS). In addition, this study examined the effects of lithium on protein levels of noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and catechol-O-methyltransferase (COMT), as well as the enzyme activity of monoamine oxidase A (MOA) in the PFC of chronically stressed rats. METHODS: The investigated parameters were quantified by Western blot analysis, CAT Research ELISA kits, and an assay of enzyme activity. Also, the forced swim test (FST) was used to assess the behavior of animals. RESULTS: We found that lithium treatment decreased high protein levels of NET and VMAT2, as well as the enzyme activity of MOA in chronically stressed rats to the levels found in unstressed animals. In addition, lithium treatment decreased the concentration of NA (24%) and immobility in animals exposed to CRS. CONCLUSION: Our data confirm that lithium-induced modulation of prefrontal noradrenergic turnover and stabilized the behavior of chronically stressed rats.
T2  - Neuroendocrinology Letters
T1  - Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats
VL  - 42
IS  - 3
SP  - 171
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9959
ER  - 

@article{
author = "Gavrilović, Ljubica and Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pantelić, Marija and Pajović, Snežana B.",
year = "2021",
abstract = "Data about the dynamics of noradrenaline (NA) transmission, storage and degradation may be very important for understanding the reduction of functional deficiency of NA and maintaining the stability of NA levels in animals with depressive-like behavior treated with lithium. This study aimed to investigate the effects of mood stabilizer lithium on concentrations of NA in the prefrontal cortex (PFC), as well as behavior rats exposed to chronic restraint stress (CRS). In addition, this study examined the effects of lithium on protein levels of noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and catechol-O-methyltransferase (COMT), as well as the enzyme activity of monoamine oxidase A (MOA) in the PFC of chronically stressed rats. METHODS: The investigated parameters were quantified by Western blot analysis, CAT Research ELISA kits, and an assay of enzyme activity. Also, the forced swim test (FST) was used to assess the behavior of animals. RESULTS: We found that lithium treatment decreased high protein levels of NET and VMAT2, as well as the enzyme activity of MOA in chronically stressed rats to the levels found in unstressed animals. In addition, lithium treatment decreased the concentration of NA (24%) and immobility in animals exposed to CRS. CONCLUSION: Our data confirm that lithium-induced modulation of prefrontal noradrenergic turnover and stabilized the behavior of chronically stressed rats.",
journal = "Neuroendocrinology Letters",
title = "Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats",
volume = "42",
number = "3",
pages = "171-176",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9959"
}

Gavrilović, L., Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I., Pantelić, M.,& Pajović, S. B.. (2021). Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats. in Neuroendocrinology Letters, 42(3), 171-176.
https://hdl.handle.net/21.15107/rcub_vinar_9959

Gavrilović L, Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Pantelić M, Pajović SB. Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats. in Neuroendocrinology Letters. 2021;42(3):171-176.
https://hdl.handle.net/21.15107/rcub_vinar_9959 .

Gavrilović, Ljubica, Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pantelić, Marija, Pajović, Snežana B., "Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats" in Neuroendocrinology Letters, 42, no. 3 (2021):171-176,
https://hdl.handle.net/21.15107/rcub_vinar_9959 .

TY  - CONF
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Đorđević, Neda
AU  - Todorović Vukotić, Nevena
AU  - Miletić, J.
AU  - Pejić, Snežana
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12580
AB  - The literature emphasizes the involvement of oxidative stress in the etiopathogenesis of many uterine diseases. Antioxidant system (AOS) represents the protective mechanism used by cells to neutralize overproduced reactive oxygen species (ROS) and prevent oxidative stress. We have previously shown that in gynecological patients with various diagnoses, the reproductive and other factors may be associated with antioxidant capacity and the ability to defend against oxidative damage. In this study, we examined the changes in expression of antioxidant enzymes (AOE): superoxide dismutases (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the blood of women with endometrial hyperplasia. Our results indicate that hyperplasia induces perturbance in oxidative balance, particularly in glutathione redox cycle enzymes.
PB  - The Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24
T1  - Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia
SP  - 308
EP  - 311
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12580
ER  - 

@conference{
author = "Todorović, Ana and Drakulić, Dunja and Gavrilović, Ljubica and Stojiljković, Vesna and Popović, Nataša M. and Đorđević, Neda and Todorović Vukotić, Nevena and Miletić, J. and Pejić, Snežana and Pajović, Snežana B.",
year = "2021",
abstract = "The literature emphasizes the involvement of oxidative stress in the etiopathogenesis of many uterine diseases. Antioxidant system (AOS) represents the protective mechanism used by cells to neutralize overproduced reactive oxygen species (ROS) and prevent oxidative stress. We have previously shown that in gynecological patients with various diagnoses, the reproductive and other factors may be associated with antioxidant capacity and the ability to defend against oxidative damage. In this study, we examined the changes in expression of antioxidant enzymes (AOE): superoxide dismutases (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the blood of women with endometrial hyperplasia. Our results indicate that hyperplasia induces perturbance in oxidative balance, particularly in glutathione redox cycle enzymes.",
publisher = "The Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24",
title = "Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia",
pages = "308-311",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12580"
}

Todorović, A., Drakulić, D., Gavrilović, L., Stojiljković, V., Popović, N. M., Đorđević, N., Todorović Vukotić, N., Miletić, J., Pejić, S.,& Pajović, S. B.. (2021). Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24
The Society of Physical Chemists of Serbia., 308-311.
https://hdl.handle.net/21.15107/rcub_vinar_12580

Todorović A, Drakulić D, Gavrilović L, Stojiljković V, Popović NM, Đorđević N, Todorović Vukotić N, Miletić J, Pejić S, Pajović SB. Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24. 2021;:308-311.
https://hdl.handle.net/21.15107/rcub_vinar_12580 .

Todorović, Ana, Drakulić, Dunja, Gavrilović, Ljubica, Stojiljković, Vesna, Popović, Nataša M., Đorđević, Neda, Todorović Vukotić, Nevena, Miletić, J., Pejić, Snežana, Pajović, Snežana B., "Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24 (2021):308-311,
https://hdl.handle.net/21.15107/rcub_vinar_12580 .

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Pejić, Snežana
AU  - Radojević-Škodrić, Sanja
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Basta-Jovanović, Gordana
AU  - Džamić, Zoran
AU  - Pajović, Snežana B.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8405
AB  - Introduction The oxidative stress contributes to all three phases of carcinogenesis and represents a concomitant condition in renal cell carcinoma (RCC). RCC is the most common type of neoplasm of the kidney, and despite numerous studies the set of predictive and prognostic markers of survival are still unknown. The aim of our study was to examine the relation between antioxidant (AO) status and overall survival (OS) in RCC patients.  Material and methods Our study included 95 patients with RCC, who underwent radical nephrectomy. We analysed the prognostic role of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, glutathione, and malondialde-hyde) and other clinicopathological factors (size, grade, stage, and histological subtype) on the OS of RCC patients.  Results The 5-year OS was 54.6%. The survival analysis related to AO parameters showed no significant difference in survival of RCC patients. The concentration of malondialdehyde, an indicator of lipid peroxidation, also had no significant effect on the survival rate of RCC pa-tients. Univariate and multivariate analysis confirmed the significance of clinicopathological parameters (size, p < 0.001; Fuhrman grade, p = 0.001, and stage, p < 0.001) for patients’ survival.  Conclusions In our cohort of patients, different antioxidant parameters were not found to be predictors for OS of patients with RCC, who underwent radical nephrectomy.
T2  - Archives of Medical Science
T1  - The effect of antioxidant status on overall survival in renal cell carcinoma
VL  - 16
IS  - 1
SP  - 94
EP  - 101
DO  - 10.5114/aoms.2019.86818
ER  - 

@article{
author = "Pavlović, Ivan and Pejić, Snežana and Radojević-Škodrić, Sanja and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Basta-Jovanović, Gordana and Džamić, Zoran and Pajović, Snežana B.",
year = "2020",
abstract = "Introduction The oxidative stress contributes to all three phases of carcinogenesis and represents a concomitant condition in renal cell carcinoma (RCC). RCC is the most common type of neoplasm of the kidney, and despite numerous studies the set of predictive and prognostic markers of survival are still unknown. The aim of our study was to examine the relation between antioxidant (AO) status and overall survival (OS) in RCC patients.  Material and methods Our study included 95 patients with RCC, who underwent radical nephrectomy. We analysed the prognostic role of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, glutathione, and malondialde-hyde) and other clinicopathological factors (size, grade, stage, and histological subtype) on the OS of RCC patients.  Results The 5-year OS was 54.6%. The survival analysis related to AO parameters showed no significant difference in survival of RCC patients. The concentration of malondialdehyde, an indicator of lipid peroxidation, also had no significant effect on the survival rate of RCC pa-tients. Univariate and multivariate analysis confirmed the significance of clinicopathological parameters (size, p < 0.001; Fuhrman grade, p = 0.001, and stage, p < 0.001) for patients’ survival.  Conclusions In our cohort of patients, different antioxidant parameters were not found to be predictors for OS of patients with RCC, who underwent radical nephrectomy.",
journal = "Archives of Medical Science",
title = "The effect of antioxidant status on overall survival in renal cell carcinoma",
volume = "16",
number = "1",
pages = "94-101",
doi = "10.5114/aoms.2019.86818"
}

Pavlović, I., Pejić, S., Radojević-Škodrić, S., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M., Basta-Jovanović, G., Džamić, Z.,& Pajović, S. B.. (2020). The effect of antioxidant status on overall survival in renal cell carcinoma. in Archives of Medical Science, 16(1), 94-101.
https://doi.org/10.5114/aoms.2019.86818

Pavlović I, Pejić S, Radojević-Škodrić S, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Basta-Jovanović G, Džamić Z, Pajović SB. The effect of antioxidant status on overall survival in renal cell carcinoma. in Archives of Medical Science. 2020;16(1):94-101.
doi:10.5114/aoms.2019.86818 .

Pavlović, Ivan, Pejić, Snežana, Radojević-Škodrić, Sanja, Todorović, Ana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Basta-Jovanović, Gordana, Džamić, Zoran, Pajović, Snežana B., "The effect of antioxidant status on overall survival in renal cell carcinoma" in Archives of Medical Science, 16, no. 1 (2020):94-101,
https://doi.org/10.5114/aoms.2019.86818 . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9617
AB  - This study aimed to investigate the effects of chronic restraint stress (CRS) on the protein levels of dopamine-β-hydroxylase (DBH), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and brain-derived neurotrophic factor (BDNF), as well as the concentration of noradrenaline (NA) in the rat hippocampus. The investigated parameters were quantified by Western blot analyses and ELISA kits. We found that CRS increased the protein levels of DBH by 30 %, VMAT2 by 11 %, BDNF by 11 % and the concentration of NA by 104 %, but decreased the protein levels of NET by 16 % in the hippocampus of chronically stressed rats. The molecular mechanisms by which CRS increased the hippocampal NA level are an important adaptive phenomenon of the noradrenergic system in the stress condition. © 2020 Charles University. All rights reserved.
T2  - Folia Biologica (Czech Republic)
T1  - Changes of Hippocampal Noradrenergic Capacity in Stress Condition
VL  - 66
IS  - 2
SP  - 81
EP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9617
ER  - 

@article{
author = "Gavrilović, Ljubica and Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pajović, Snežana B.",
year = "2020",
abstract = "This study aimed to investigate the effects of chronic restraint stress (CRS) on the protein levels of dopamine-β-hydroxylase (DBH), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and brain-derived neurotrophic factor (BDNF), as well as the concentration of noradrenaline (NA) in the rat hippocampus. The investigated parameters were quantified by Western blot analyses and ELISA kits. We found that CRS increased the protein levels of DBH by 30 %, VMAT2 by 11 %, BDNF by 11 % and the concentration of NA by 104 %, but decreased the protein levels of NET by 16 % in the hippocampus of chronically stressed rats. The molecular mechanisms by which CRS increased the hippocampal NA level are an important adaptive phenomenon of the noradrenergic system in the stress condition. © 2020 Charles University. All rights reserved.",
journal = "Folia Biologica (Czech Republic)",
title = "Changes of Hippocampal Noradrenergic Capacity in Stress Condition",
volume = "66",
number = "2",
pages = "81-84",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9617"
}

Gavrilović, L., Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I.,& Pajović, S. B.. (2020). Changes of Hippocampal Noradrenergic Capacity in Stress Condition. in Folia Biologica (Czech Republic), 66(2), 81-84.
https://hdl.handle.net/21.15107/rcub_vinar_9617

Gavrilović L, Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Pajović SB. Changes of Hippocampal Noradrenergic Capacity in Stress Condition. in Folia Biologica (Czech Republic). 2020;66(2):81-84.
https://hdl.handle.net/21.15107/rcub_vinar_9617 .

Gavrilović, Ljubica, Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pajović, Snežana B., "Changes of Hippocampal Noradrenergic Capacity in Stress Condition" in Folia Biologica (Czech Republic), 66, no. 2 (2020):81-84,
https://hdl.handle.net/21.15107/rcub_vinar_9617 .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
AU  - Pajović, Snežana B.
PY  - 2019
UR  - https://www.hindawi.com/journals/omcl/2019/8745376/
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8085
AB  - This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium
VL  - 2019
SP  - 1
EP  - 11
DO  - 10.1155/2019/8745376
ER  - 

@article{
author = "Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Gavrilović, Ljubica and Pajović, Snežana B.",
year = "2019",
abstract = "This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium",
volume = "2019",
pages = "1-11",
doi = "10.1155/2019/8745376"
}

Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I., Gavrilović, L.,& Pajović, S. B.. (2019). Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium. in Oxidative Medicine and Cellular Longevity, 2019, 1-11.
https://doi.org/10.1155/2019/8745376

Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Gavrilović L, Pajović SB. Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium. in Oxidative Medicine and Cellular Longevity. 2019;2019:1-11.
doi:10.1155/2019/8745376 .

Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Gavrilović, Ljubica, Pajović, Snežana B., "Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium" in Oxidative Medicine and Cellular Longevity, 2019 (2019):1-11,
https://doi.org/10.1155/2019/8745376 . .

TY  - JOUR
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Gavrilović, Ljubica
AU  - Pavlović, Ivan
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2019
UR  - https://www.mdpi.com/2076-3921/8/4/97
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8403
AB  - We previously found that compared to patients with benign uterine diseases (polyps, myomas), patients with premalignant (hyperplasia simplex and complex) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme (AOE) activities. To further elucidate the mechanism of the observed changes, we examined protein and mRNA levels of copper-zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and transcription factor Nrf2. We also examined correlations of AOE expression with AOE activity, lipid hydroperoxides (LOOH) level, and level of Nrf2. Our results showed decreased CuZnSOD, CAT, and Nrf2 levels, and increased GPx and GR levels in hyperplasias, while in patients with adenocarcinoma, the level of CAT was decreased and GR was increased, compared to benign groups. Similar changes in mRNA levels were also detected, indicating predominantly translational control of the AOE expression. The positive correlation of enzyme expression/activity was recorded for CuZnSOD, GPx, and GR, but only among groups with benign diseases. Only GR and GPx expressions were positively correlated with LOOH. Nrf2 protein was positively correlated with mRNA levels of CuZnSOD and GR. Observed results indicate involvement of diverse redox mechanisms in etiopathogenesis of different gynecological diseases, and may improve redox-based approaches in current clinical practice.
T2  - Antioxidants
T1  - Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma
VL  - 8
IS  - 4
SP  - 97
DO  - 10.3390/antiox8040097
ER  - 

@article{
author = "Todorović, Ana and Pejić, Snežana and Gavrilović, Ljubica and Pavlović, Ivan and Stojiljković, Vesna and Popović, Nataša M. and Pajović, Snežana B.",
year = "2019",
abstract = "We previously found that compared to patients with benign uterine diseases (polyps, myomas), patients with premalignant (hyperplasia simplex and complex) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme (AOE) activities. To further elucidate the mechanism of the observed changes, we examined protein and mRNA levels of copper-zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and transcription factor Nrf2. We also examined correlations of AOE expression with AOE activity, lipid hydroperoxides (LOOH) level, and level of Nrf2. Our results showed decreased CuZnSOD, CAT, and Nrf2 levels, and increased GPx and GR levels in hyperplasias, while in patients with adenocarcinoma, the level of CAT was decreased and GR was increased, compared to benign groups. Similar changes in mRNA levels were also detected, indicating predominantly translational control of the AOE expression. The positive correlation of enzyme expression/activity was recorded for CuZnSOD, GPx, and GR, but only among groups with benign diseases. Only GR and GPx expressions were positively correlated with LOOH. Nrf2 protein was positively correlated with mRNA levels of CuZnSOD and GR. Observed results indicate involvement of diverse redox mechanisms in etiopathogenesis of different gynecological diseases, and may improve redox-based approaches in current clinical practice.",
journal = "Antioxidants",
title = "Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma",
volume = "8",
number = "4",
pages = "97",
doi = "10.3390/antiox8040097"
}

Todorović, A., Pejić, S., Gavrilović, L., Pavlović, I., Stojiljković, V., Popović, N. M.,& Pajović, S. B.. (2019). Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma. in Antioxidants, 8(4), 97.
https://doi.org/10.3390/antiox8040097

Todorović A, Pejić S, Gavrilović L, Pavlović I, Stojiljković V, Popović NM, Pajović SB. Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma. in Antioxidants. 2019;8(4):97.
doi:10.3390/antiox8040097 .

Todorović, Ana, Pejić, Snežana, Gavrilović, Ljubica, Pavlović, Ivan, Stojiljković, Vesna, Popović, Nataša M., Pajović, Snežana B., "Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma" in Antioxidants, 8, no. 4 (2019):97,
https://doi.org/10.3390/antiox8040097 . .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8404
AB  - The effects of chronic restraint stress (CRS, 2 h during 14 days) on gene expression of tyrosine hydroxylase (TH), catechol-O-methyltransferase (COMT), and glutathione peroxidase (GPx) were studied in the rat hippocampus. Changes in the dopamine (DA) concentration and activities of monoamine oxidases (MAO A and MAO B) and GPx in this cerebral structure of chronically stressed rats were also examined. The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assay of enzymatic activity. We found that CRS decreased the TH protein level and DA concentration, which probably confirms the statement that de novo synthesis of DA is suppressed under stress conditions. The increased activities of MAO B, as well as the increased level of COMT protein, are believed to be related to intensified DA catabolism conditions. Also, a decreased activity of GPx in the hippocampus of chronically stressed animals was found. The increased enzymatic activity of MAO B negatively correlated with the reduced activity of GPx under the above-mentioned stress conditions. These events in the hippocampus of chronically stressed animals could synergistically cause oxidative damage to the mitochondria. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Neurophysiology
T1  - Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats
VL  - 50
IS  - 5
SP  - 332
EP  - 338
DO  - 10.1007/s11062-019-09758-z
ER  - 

@article{
author = "Popović, Nataša M. and Pajović, Snežana B. and Stojiljković, Vesna and Todorović, Ana and Pejić, Snežana and Pavlović, Ivan and Gavrilović, Ljubica",
year = "2018",
abstract = "The effects of chronic restraint stress (CRS, 2 h during 14 days) on gene expression of tyrosine hydroxylase (TH), catechol-O-methyltransferase (COMT), and glutathione peroxidase (GPx) were studied in the rat hippocampus. Changes in the dopamine (DA) concentration and activities of monoamine oxidases (MAO A and MAO B) and GPx in this cerebral structure of chronically stressed rats were also examined. The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assay of enzymatic activity. We found that CRS decreased the TH protein level and DA concentration, which probably confirms the statement that de novo synthesis of DA is suppressed under stress conditions. The increased activities of MAO B, as well as the increased level of COMT protein, are believed to be related to intensified DA catabolism conditions. Also, a decreased activity of GPx in the hippocampus of chronically stressed animals was found. The increased enzymatic activity of MAO B negatively correlated with the reduced activity of GPx under the above-mentioned stress conditions. These events in the hippocampus of chronically stressed animals could synergistically cause oxidative damage to the mitochondria. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Neurophysiology",
title = "Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats",
volume = "50",
number = "5",
pages = "332-338",
doi = "10.1007/s11062-019-09758-z"
}

Popović, N. M., Pajović, S. B., Stojiljković, V., Todorović, A., Pejić, S., Pavlović, I.,& Gavrilović, L.. (2018). Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats. in Neurophysiology, 50(5), 332-338.
https://doi.org/10.1007/s11062-019-09758-z

Popović NM, Pajović SB, Stojiljković V, Todorović A, Pejić S, Pavlović I, Gavrilović L. Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats. in Neurophysiology. 2018;50(5):332-338.
doi:10.1007/s11062-019-09758-z .

Popović, Nataša M., Pajović, Snežana B., Stojiljković, Vesna, Todorović, Ana, Pejić, Snežana, Pavlović, Ivan, Gavrilović, Ljubica, "Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats" in Neurophysiology, 50, no. 5 (2018):332-338,
https://doi.org/10.1007/s11062-019-09758-z . .

TY  - CHAP
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8406
AB  - We examined the effects of daily exercise on the gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), and phenyl
ethanolamine N-methyltransferase (PNMT)), vesicular monoamine transporter 2 (VMAT 2),
antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), concentrations of catecholamines (noradrenaline (NA) and adrenaline (A))
and malondialdehyde (MDA), activities of monoamine oxidase (MAO), and antioxidant
enzymes in the spleen of chronically psychosocially stressed rats. Exposure of chronically
stressed rats to exercise increased the levels of PNMT protein by 19%, VMAT 2 mRNA by
100%, NA by 160%, and A by 140%; decreased/unchanged MAO enzyme activity; returned
concentrations of MDA to control level; and increased CAT and GPx mRNA levels (50%
and 150%, respectively). Exercise induced the accumulation of the catecholamines and a
decrease of stress-induced oxidative stress in the spleen, which may significantly affect the
immune-neuroendocrine interactions in stress conditions. Also, exercise induced the catecholaminergic system and antioxidant defense to become more ready to a novel stressor,
which indicates that exercise may induce potentially positive physiological adaptations.
Our combined model of chronic social isolation and long-term daily treadmill running in
rats may be a good animal model in the research of therapeutic role of exercise in human
disease caused by chronic stress.
PB  - IntechOpen
PB  - InTech
T2  - Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh
T1  - Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System
IS  - Ch. 14
SP  - 283
EP  - 310
DO  - 10.5772/intechopen.70008
ER  - 

@inbook{
editor = "Bartholomew, Ibeh",
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Popović, Nataša M. and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pajović, Snežana B.",
year = "2018",
abstract = "We examined the effects of daily exercise on the gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), and phenyl
ethanolamine N-methyltransferase (PNMT)), vesicular monoamine transporter 2 (VMAT 2),
antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), concentrations of catecholamines (noradrenaline (NA) and adrenaline (A))
and malondialdehyde (MDA), activities of monoamine oxidase (MAO), and antioxidant
enzymes in the spleen of chronically psychosocially stressed rats. Exposure of chronically
stressed rats to exercise increased the levels of PNMT protein by 19%, VMAT 2 mRNA by
100%, NA by 160%, and A by 140%; decreased/unchanged MAO enzyme activity; returned
concentrations of MDA to control level; and increased CAT and GPx mRNA levels (50%
and 150%, respectively). Exercise induced the accumulation of the catecholamines and a
decrease of stress-induced oxidative stress in the spleen, which may significantly affect the
immune-neuroendocrine interactions in stress conditions. Also, exercise induced the catecholaminergic system and antioxidant defense to become more ready to a novel stressor,
which indicates that exercise may induce potentially positive physiological adaptations.
Our combined model of chronic social isolation and long-term daily treadmill running in
rats may be a good animal model in the research of therapeutic role of exercise in human
disease caused by chronic stress.",
publisher = "IntechOpen, InTech",
journal = "Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh",
booktitle = "Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System",
number = "Ch. 14",
pages = "283-310",
doi = "10.5772/intechopen.70008"
}

Bartholomew, I., Gavrilović, L., Stojiljković, V., Popović, N. M., Pejić, S., Todorović, A., Pavlović, I.,& Pajović, S. B.. (2018). Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System. in Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh
IntechOpen.(Ch. 14), 283-310.
https://doi.org/10.5772/intechopen.70008

Bartholomew I, Gavrilović L, Stojiljković V, Popović NM, Pejić S, Todorović A, Pavlović I, Pajović SB. Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System. in Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh. 2018;(Ch. 14):283-310.
doi:10.5772/intechopen.70008 .

Bartholomew, Ibeh, Gavrilović, Ljubica, Stojiljković, Vesna, Popović, Nataša M., Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pajović, Snežana B., "Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System" in Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh, no. Ch. 14 (2018):283-310,
https://doi.org/10.5772/intechopen.70008 . .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1588
AB  - This study examined the effects of chronic restraint stress (CRS) on gene expression of tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH), dopamine transporter (DAT), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2), catechol-O-methyltransferase (COMT) and antioxidant enzymes such as superoxide dismutase (SOD1 and SOD2), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), as well as concentrations of dopamine (DA) and noradrenaline (NA), activities of monoamine oxidase (MAO A and MAOB) and activities of antioxidant enzymes (SOD1, SOD2, CAT, GPx and GR) in the rat prefrontal cortex (PFC). We found that CRS decreases gene expression of TH and DBH and concentrations of DA, which probably confirms the decrease of de novo synthesis of catecholamine. CRS increased protein levels of NET and VMAT2, which was followed by increased NA concentration. The increased activity of MAO A and MAO B, as well as increased protein levels of COMT probably indicate increased catecholamine degradation, which was followed by increased activity of SOD1, SOD2 and CAT, as well as decreased activity of GPx under stress conditions. Our findings confirm the increased prefrontal noradrenergic turnover in animals exposed to CRS. The molecular mechanisms by which CRS changes catecholaminergic turnover and the antioxidant defense system in the PFC may be very important for research on numerous psychiatric diseases caused by chronic stress.
T2  - Folia Biologica (Krakow)
T1  - Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats
VL  - 65
IS  - 1
SP  - 43
EP  - 54
DO  - 10.3409/fb65_1.43
ER  - 

@article{
author = "Popović, Nataša M. and Pajović, Snežana B. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Gavrilović, Ljubica",
year = "2017",
abstract = "This study examined the effects of chronic restraint stress (CRS) on gene expression of tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH), dopamine transporter (DAT), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2), catechol-O-methyltransferase (COMT) and antioxidant enzymes such as superoxide dismutase (SOD1 and SOD2), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), as well as concentrations of dopamine (DA) and noradrenaline (NA), activities of monoamine oxidase (MAO A and MAOB) and activities of antioxidant enzymes (SOD1, SOD2, CAT, GPx and GR) in the rat prefrontal cortex (PFC). We found that CRS decreases gene expression of TH and DBH and concentrations of DA, which probably confirms the decrease of de novo synthesis of catecholamine. CRS increased protein levels of NET and VMAT2, which was followed by increased NA concentration. The increased activity of MAO A and MAO B, as well as increased protein levels of COMT probably indicate increased catecholamine degradation, which was followed by increased activity of SOD1, SOD2 and CAT, as well as decreased activity of GPx under stress conditions. Our findings confirm the increased prefrontal noradrenergic turnover in animals exposed to CRS. The molecular mechanisms by which CRS changes catecholaminergic turnover and the antioxidant defense system in the PFC may be very important for research on numerous psychiatric diseases caused by chronic stress.",
journal = "Folia Biologica (Krakow)",
title = "Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats",
volume = "65",
number = "1",
pages = "43-54",
doi = "10.3409/fb65_1.43"
}

Popović, N. M., Pajović, S. B., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I.,& Gavrilović, L.. (2017). Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats. in Folia Biologica (Krakow), 65(1), 43-54.
https://doi.org/10.3409/fb65_1.43

Popović NM, Pajović SB, Stojiljković V, Pejić S, Todorović A, Pavlović I, Gavrilović L. Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats. in Folia Biologica (Krakow). 2017;65(1):43-54.
doi:10.3409/fb65_1.43 .

Popović, Nataša M., Pajović, Snežana B., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Gavrilović, Ljubica, "Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats" in Folia Biologica (Krakow), 65, no. 1 (2017):43-54,
https://doi.org/10.3409/fb65_1.43 . .

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Pejić, Snežana
AU  - Glumac, Sofija
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Gavrilović, Ljubica
AU  - Pajović, Snežana B.
AU  - Radojević-Škodrić, Sanja
AU  - Džamić, Zoran
AU  - Basta-Jovanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1857
AB  - Purpose: Indications of kidney cancer outcome in lower-income countries are based on an incidence/mortality ratio due to lack of survival information. This study was conducted to provide outcome data in Serbian patients with renal cell carcinoma (RCC) and to identify prognostic factors that could affect their overall survival (OS). Methods: This retrospective study included 185 patients who underwent nephrectomy. We assessed certain clinicopathological data including age, gender, tumor size, grade, stage and histological subtypes for their possible impact on OS. Results: The 5-year OS was 63.2%. Significant association was found between OS and age (log-rank 12.455, p=0.006), tumor size (log-rank 26.425, p=0.000), grade (log-rank 13.249, p=0.000) and stage (log-rank 43.235, p=0.000). Univariate analysis indicated size (p=0.000), grade (p=0.001) and stage (p=0.000) as prognostic factors for OS. In multivariate analysis, grade (p=0.014) and stage (p=0.000) remained significant predictors of OS. Conclusion: Tumor grade and stage were identified as independent prognostic factors of OS survival in Serbian patients with RCC.
T2  - Journal of BUON
T1  - Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis
VL  - 22
IS  - 6
SP  - 1434
EP  - 1440
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1857
ER  - 

@article{
author = "Pavlović, Ivan and Pejić, Snežana and Glumac, Sofija and Todorović, Ana and Stojiljković, Vesna and Popović, Nataša M. and Gavrilović, Ljubica and Pajović, Snežana B. and Radojević-Škodrić, Sanja and Džamić, Zoran and Basta-Jovanović, Gordana",
year = "2017",
abstract = "Purpose: Indications of kidney cancer outcome in lower-income countries are based on an incidence/mortality ratio due to lack of survival information. This study was conducted to provide outcome data in Serbian patients with renal cell carcinoma (RCC) and to identify prognostic factors that could affect their overall survival (OS). Methods: This retrospective study included 185 patients who underwent nephrectomy. We assessed certain clinicopathological data including age, gender, tumor size, grade, stage and histological subtypes for their possible impact on OS. Results: The 5-year OS was 63.2%. Significant association was found between OS and age (log-rank 12.455, p=0.006), tumor size (log-rank 26.425, p=0.000), grade (log-rank 13.249, p=0.000) and stage (log-rank 43.235, p=0.000). Univariate analysis indicated size (p=0.000), grade (p=0.001) and stage (p=0.000) as prognostic factors for OS. In multivariate analysis, grade (p=0.014) and stage (p=0.000) remained significant predictors of OS. Conclusion: Tumor grade and stage were identified as independent prognostic factors of OS survival in Serbian patients with RCC.",
journal = "Journal of BUON",
title = "Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis",
volume = "22",
number = "6",
pages = "1434-1440",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1857"
}

Pavlović, I., Pejić, S., Glumac, S., Todorović, A., Stojiljković, V., Popović, N. M., Gavrilović, L., Pajović, S. B., Radojević-Škodrić, S., Džamić, Z.,& Basta-Jovanović, G.. (2017). Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis. in Journal of BUON, 22(6), 1434-1440.
https://hdl.handle.net/21.15107/rcub_vinar_1857

Pavlović I, Pejić S, Glumac S, Todorović A, Stojiljković V, Popović NM, Gavrilović L, Pajović SB, Radojević-Škodrić S, Džamić Z, Basta-Jovanović G. Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis. in Journal of BUON. 2017;22(6):1434-1440.
https://hdl.handle.net/21.15107/rcub_vinar_1857 .

Pavlović, Ivan, Pejić, Snežana, Glumac, Sofija, Todorović, Ana, Stojiljković, Vesna, Popović, Nataša M., Gavrilović, Ljubica, Pajović, Snežana B., Radojević-Škodrić, Sanja, Džamić, Zoran, Basta-Jovanović, Gordana, "Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis" in Journal of BUON, 22, no. 6 (2017):1434-1440,
https://hdl.handle.net/21.15107/rcub_vinar_1857 .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1908
AB  - This study examined the effects of chronic restraint stress (CRS: 2 hours x 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantified by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our findings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.
T2  - Acta Veterinaria, Beograd
T1  - Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats
VL  - 67
IS  - 4
SP  - 540
EP  - 550
DO  - 10.1515/acve-2017-0043
ER  - 

@article{
author = "Popović, Nataša M. and Pajović, Snežana B. and Stojiljković, Vesna and Todorović, Ana and Pejić, Snežana and Pavlović, Ivan and Gavrilović, Ljubica",
year = "2017",
abstract = "This study examined the effects of chronic restraint stress (CRS: 2 hours x 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantified by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our findings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.",
journal = "Acta Veterinaria, Beograd",
title = "Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats",
volume = "67",
number = "4",
pages = "540-550",
doi = "10.1515/acve-2017-0043"
}

Popović, N. M., Pajović, S. B., Stojiljković, V., Todorović, A., Pejić, S., Pavlović, I.,& Gavrilović, L.. (2017). Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats. in Acta Veterinaria, Beograd, 67(4), 540-550.
https://doi.org/10.1515/acve-2017-0043

Popović NM, Pajović SB, Stojiljković V, Todorović A, Pejić S, Pavlović I, Gavrilović L. Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats. in Acta Veterinaria, Beograd. 2017;67(4):540-550.
doi:10.1515/acve-2017-0043 .

Popović, Nataša M., Pajović, Snežana B., Stojiljković, Vesna, Todorović, Ana, Pejić, Snežana, Pavlović, Ivan, Gavrilović, Ljubica, "Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats" in Acta Veterinaria, Beograd, 67, no. 4 (2017):540-550,
https://doi.org/10.1515/acve-2017-0043 . .

TY  - CONF
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2017
UR  - http://www.rad-proceedings.org/paper.php?id=152
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7995
AB  - Celiac disease (CD) is an autoimmune disorder provoked by the wheat gluten and related proteins from other grains. The only treatment for the patients is a lifelong gluten free diet (GFD). Oxidative stress has been implicated in the pathogenesis of CD. The aim of this study was to examine the modulation of the biochemical response to oxidative stress in children affected by CD. The study involved peripheral blood samples and small intestinal biopsies from 69 children diagnosed with CD. According to the histological findings, patients were divided into following groups: Marsh 0: normal mucosa with no signs of inflammation (n=31); Marsh 1: mucosa was characterized by intraepithelial lymphocytosis (n=5); Marsh 2: intraepithelial lymphocytosis was accompanied by crypt hyperplasia (n=4); Marsh 3a: mucosa showed partial villous atrophy (n=20); Marsh 3b: subtotal villous atrophy was present (n=9). For the statistical purposes groups Marsh 1 and Marsh 2 were treated as one (Marsh 1+2, n=9). The activities and protein levels of copper, zinc superoxide dismutase (CuZnSOD) and manganese SOD (MnSOD), as well as the concentrations of lipid hydroperoxides (LOOH) were determined in intestinal biopsies, while in the peripheral blood, MnSOD activity was not measured, due to the methodological obstacles. CuZnSOD activity in the blood varied significantly between the analyzed groups. Marsh 3a and Marsh 3b had increased CuZnSOD activity comparing to the Marsh 0 (P < 0.05). LOOH concentration also varied significantly. LOOH level was higher in the blood of Marsh 3a (P < 0.001) and Marsh 3b (P < 0.05), than in Marsh 0. In the biopsy samples, MnSOD activity and LOOH concentration showed significant differences between the groups, while no significant difference was found for CuZnSOD activity. In comparison to Marsh 0, MnSOD activity was significantly elevated in Marsh 3a (P < 0.01). Significant increase in LOOH concentration was found in Marsh 3a (P < 0.001) and Marsh 3b (P < 0.01), comparing to Marsh 0. In addition, Marsh 3a group had higher LOOH concentration than Marsh 1+2. Relative MnSOD and CuZnSOD protein level in the peripheral blood and intestinal mucosa did not vary significantly between the analyzed groups. Positive correlations were found between the severity of mucosal lesion and CuZnSOD activity (P < 0.001), as well as LOOH concentration (P < 0.001) in the peripheral blood. Similar correlations were found also in intestinal mucosa: MnSOD: P < 0.05; CuZnSOD: P < 0.05; LOOH: P < 0.001. Our results show that oxidant/antioxidant balance is disturbed in CD patients with mucosal lesions. An increase in SOD activity as a consequence of oxidant pressure is not enough to maintain the normal level of free radicals, which leads to enhanced lipid peroxidation. These processes persist even in some patients on a long-term GFD.
PB  - RAD Association
C3  - RAD Conference Proceedings
T1  - Superoxide dismutase and lipid peroxidation in children affected by celiac disease
VL  - 2
SP  - 237
EP  - 242
DO  - 10.21175/RadProc.2017.49
ER  - 

@conference{
author = "Stojiljković, Vesna and Gavrilović, Ljubica and Pejić, Snežana and Todorović, Ana and Popović, Nataša M. and Pavlović, Ivan and Pajović, Snežana B.",
year = "2017",
abstract = "Celiac disease (CD) is an autoimmune disorder provoked by the wheat gluten and related proteins from other grains. The only treatment for the patients is a lifelong gluten free diet (GFD). Oxidative stress has been implicated in the pathogenesis of CD. The aim of this study was to examine the modulation of the biochemical response to oxidative stress in children affected by CD. The study involved peripheral blood samples and small intestinal biopsies from 69 children diagnosed with CD. According to the histological findings, patients were divided into following groups: Marsh 0: normal mucosa with no signs of inflammation (n=31); Marsh 1: mucosa was characterized by intraepithelial lymphocytosis (n=5); Marsh 2: intraepithelial lymphocytosis was accompanied by crypt hyperplasia (n=4); Marsh 3a: mucosa showed partial villous atrophy (n=20); Marsh 3b: subtotal villous atrophy was present (n=9). For the statistical purposes groups Marsh 1 and Marsh 2 were treated as one (Marsh 1+2, n=9). The activities and protein levels of copper, zinc superoxide dismutase (CuZnSOD) and manganese SOD (MnSOD), as well as the concentrations of lipid hydroperoxides (LOOH) were determined in intestinal biopsies, while in the peripheral blood, MnSOD activity was not measured, due to the methodological obstacles. CuZnSOD activity in the blood varied significantly between the analyzed groups. Marsh 3a and Marsh 3b had increased CuZnSOD activity comparing to the Marsh 0 (P < 0.05). LOOH concentration also varied significantly. LOOH level was higher in the blood of Marsh 3a (P < 0.001) and Marsh 3b (P < 0.05), than in Marsh 0. In the biopsy samples, MnSOD activity and LOOH concentration showed significant differences between the groups, while no significant difference was found for CuZnSOD activity. In comparison to Marsh 0, MnSOD activity was significantly elevated in Marsh 3a (P < 0.01). Significant increase in LOOH concentration was found in Marsh 3a (P < 0.001) and Marsh 3b (P < 0.01), comparing to Marsh 0. In addition, Marsh 3a group had higher LOOH concentration than Marsh 1+2. Relative MnSOD and CuZnSOD protein level in the peripheral blood and intestinal mucosa did not vary significantly between the analyzed groups. Positive correlations were found between the severity of mucosal lesion and CuZnSOD activity (P < 0.001), as well as LOOH concentration (P < 0.001) in the peripheral blood. Similar correlations were found also in intestinal mucosa: MnSOD: P < 0.05; CuZnSOD: P < 0.05; LOOH: P < 0.001. Our results show that oxidant/antioxidant balance is disturbed in CD patients with mucosal lesions. An increase in SOD activity as a consequence of oxidant pressure is not enough to maintain the normal level of free radicals, which leads to enhanced lipid peroxidation. These processes persist even in some patients on a long-term GFD.",
publisher = "RAD Association",
journal = "RAD Conference Proceedings",
title = "Superoxide dismutase and lipid peroxidation in children affected by celiac disease",
volume = "2",
pages = "237-242",
doi = "10.21175/RadProc.2017.49"
}

Stojiljković, V., Gavrilović, L., Pejić, S., Todorović, A., Popović, N. M., Pavlović, I.,& Pajović, S. B.. (2017). Superoxide dismutase and lipid peroxidation in children affected by celiac disease. in RAD Conference Proceedings
RAD Association., 2, 237-242.
https://doi.org/10.21175/RadProc.2017.49

Stojiljković V, Gavrilović L, Pejić S, Todorović A, Popović NM, Pavlović I, Pajović SB. Superoxide dismutase and lipid peroxidation in children affected by celiac disease. in RAD Conference Proceedings. 2017;2:237-242.
doi:10.21175/RadProc.2017.49 .

Stojiljković, Vesna, Gavrilović, Ljubica, Pejić, Snežana, Todorović, Ana, Popović, Nataša M., Pavlović, Ivan, Pajović, Snežana B., "Superoxide dismutase and lipid peroxidation in children affected by celiac disease" in RAD Conference Proceedings, 2 (2017):237-242,
https://doi.org/10.21175/RadProc.2017.49 . .

TY  - CONF
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
PY  - 2017
UR  - http://www.rad-proceedings.org/paper.php?id=155
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7996
AB  - Chronic stress induces over-activation and dysfunction of stress-activated systems, resulting in further brain damage and depressive-like behavior. Depression is a potentially life-threatening disorder that affects people and, therefore, it is one of the most important public health problems. This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on the anxiety-like and depression-like behaviors in rats, as well as on the possible changes in the concentrations of dopamine (DA) and noradrenaline (NA) in the prefrontal cortex and hippocampus. We observed a decrease in the number of entries into open arms and time spent in open arms during the elevated plus-maze test (anxiety-like behavior), as well as the increased immobility during the forced swimming test (depression-like behavior). In addition, we found that CRS increases concentration of NA and decreases concentration of DA in the prefrontal cortex and hippocampus. Also, we recorded a significant correlation between the animal behavior and levels of neurotransmitters in the prefrontal cortex and hippocampus in stress conditions provoked by CRS. The results presented here suggest that there is a relationship between the animal behavior and levels of neurotransmitters in the prefrontal cortex and hippocampus in stress conditions provoked by CRS, which may be important in the research of numerous psychiatric diseases caused by chronic stress. © RAD Conference Proceedings. All rights reserved.
PB  - RAD Association
C3  - RAD Conference Proceedings
T1  - Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats
VL  - 2
SP  - 255
EP  - 259
DO  - 10.21175/RadProc.2017.52
ER  - 

@conference{
author = "Popović, Nataša M. and Pajović, Snežana B. and Stojiljković, Vesna and Todorović, Ana and Pejić, Snežana and Pavlović, Ivan and Gavrilović, Ljubica",
year = "2017",
abstract = "Chronic stress induces over-activation and dysfunction of stress-activated systems, resulting in further brain damage and depressive-like behavior. Depression is a potentially life-threatening disorder that affects people and, therefore, it is one of the most important public health problems. This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on the anxiety-like and depression-like behaviors in rats, as well as on the possible changes in the concentrations of dopamine (DA) and noradrenaline (NA) in the prefrontal cortex and hippocampus. We observed a decrease in the number of entries into open arms and time spent in open arms during the elevated plus-maze test (anxiety-like behavior), as well as the increased immobility during the forced swimming test (depression-like behavior). In addition, we found that CRS increases concentration of NA and decreases concentration of DA in the prefrontal cortex and hippocampus. Also, we recorded a significant correlation between the animal behavior and levels of neurotransmitters in the prefrontal cortex and hippocampus in stress conditions provoked by CRS. The results presented here suggest that there is a relationship between the animal behavior and levels of neurotransmitters in the prefrontal cortex and hippocampus in stress conditions provoked by CRS, which may be important in the research of numerous psychiatric diseases caused by chronic stress. © RAD Conference Proceedings. All rights reserved.",
publisher = "RAD Association",
journal = "RAD Conference Proceedings",
title = "Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats",
volume = "2",
pages = "255-259",
doi = "10.21175/RadProc.2017.52"
}

Popović, N. M., Pajović, S. B., Stojiljković, V., Todorović, A., Pejić, S., Pavlović, I.,& Gavrilović, L.. (2017). Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats. in RAD Conference Proceedings
RAD Association., 2, 255-259.
https://doi.org/10.21175/RadProc.2017.52

Popović NM, Pajović SB, Stojiljković V, Todorović A, Pejić S, Pavlović I, Gavrilović L. Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats. in RAD Conference Proceedings. 2017;2:255-259.
doi:10.21175/RadProc.2017.52 .

Popović, Nataša M., Pajović, Snežana B., Stojiljković, Vesna, Todorović, Ana, Pejić, Snežana, Pavlović, Ivan, Gavrilović, Ljubica, "Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats" in RAD Conference Proceedings, 2 (2017):255-259,
https://doi.org/10.21175/RadProc.2017.52 . .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Gavrilović, Ljubica
AU  - Pavlović, Ivan
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1301
AB  - The study deals with manganese superoxide dismutase, copper, zinc superoxide dismutase, and catalase activities in brain cortex of Wistar rats exposed to acute stress (immobilization or cold for 2 h), chronic stress (long-term isolation or long-term forced swimming for 21 days), or to combined chronic/acute stress. We observed that i) single episodes of acute stress by immobilization increased activity of both superoxide dismutases; ii) both types of chronic stresses significantly elevated activities of all examined enzymes; iii) chronic social isolation was a much stronger stressor than physical stress by swimming; iv) in animals pre-exposed to chronic isolation, additional stress by immobilization or cold significantly decreased previously elevated activities of all enzymes, while after chronic swimming, acute immobilization lowered only catalase activity. The obtained results indicate that stress conditions most probably altered the cell redox equilibrium, thus influencing the antioxidant response in brain cortex. Further investigation of neuronal prooxidant/antioxidant cellular conditions is needed to improve the prevention and treatment of various stress induced diseases.
T2  - Folia Biologica (Krakow)
T1  - Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress
VL  - 64
IS  - 3
SP  - 189
EP  - 195
DO  - 10.3409/fb64_3.189
ER  - 

@article{
author = "Pejić, Snežana and Stojiljković, Vesna and Todorović, Ana and Gavrilović, Ljubica and Pavlović, Ivan and Popović, Nataša M. and Pajović, Snežana B.",
year = "2016",
abstract = "The study deals with manganese superoxide dismutase, copper, zinc superoxide dismutase, and catalase activities in brain cortex of Wistar rats exposed to acute stress (immobilization or cold for 2 h), chronic stress (long-term isolation or long-term forced swimming for 21 days), or to combined chronic/acute stress. We observed that i) single episodes of acute stress by immobilization increased activity of both superoxide dismutases; ii) both types of chronic stresses significantly elevated activities of all examined enzymes; iii) chronic social isolation was a much stronger stressor than physical stress by swimming; iv) in animals pre-exposed to chronic isolation, additional stress by immobilization or cold significantly decreased previously elevated activities of all enzymes, while after chronic swimming, acute immobilization lowered only catalase activity. The obtained results indicate that stress conditions most probably altered the cell redox equilibrium, thus influencing the antioxidant response in brain cortex. Further investigation of neuronal prooxidant/antioxidant cellular conditions is needed to improve the prevention and treatment of various stress induced diseases.",
journal = "Folia Biologica (Krakow)",
title = "Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress",
volume = "64",
number = "3",
pages = "189-195",
doi = "10.3409/fb64_3.189"
}

Pejić, S., Stojiljković, V., Todorović, A., Gavrilović, L., Pavlović, I., Popović, N. M.,& Pajović, S. B.. (2016). Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress. in Folia Biologica (Krakow), 64(3), 189-195.
https://doi.org/10.3409/fb64_3.189

Pejić S, Stojiljković V, Todorović A, Gavrilović L, Pavlović I, Popović NM, Pajović SB. Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress. in Folia Biologica (Krakow). 2016;64(3):189-195.
doi:10.3409/fb64_3.189 .

Pejić, Snežana, Stojiljković, Vesna, Todorović, Ana, Gavrilović, Ljubica, Pavlović, Ivan, Popović, Nataša M., Pajović, Snežana B., "Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress" in Folia Biologica (Krakow), 64, no. 3 (2016):189-195,
https://doi.org/10.3409/fb64_3.189 . .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1455
AB  - Endometrial tissue is under a strong influence of sex hormones. These hormones are considered as developmental factors of endometrial hyperplasia and endometrial cancer. We examined the influence of gonadotropins (follicle-stimulating and luteinizing hormone) and sex hormones (estradiol, progesterone) on oxidant/antioxidant parameters in blood and endometrial tissue of women with complex endometrial hyperplasia. In blood, superoxide dismutase activity was significantly higher in luteal phase and postmenopause compared to the follicular phase. A significant phase-related difference of glutathione peroxidase and glutathione reductase activity was recorded in the endometrium. Both enzymes had lower activity in luteal phase and postmenopause compared to the follicular phase. The linear regression analysis of individual hormonal variables against antioxidant parameters showed negative correlation between glutathione peroxidase activity and gonadotropin concentrations in the endometrium. The regression of hyperplastic to normal endometrium is the purpose of conservative treatment based on administration of progestogens or gonadotropin-releasing hormone analogues. Our findings indicate that gonadotropins influence the antioxidant enzymes activity in women with complex endometrial hyperplasia, which may affect disease development. Further studies are needed to clarify the molecular basis of hormone action on antioxidant system that may potentially initiate a development of treatments based onredox-dependentmechanism.
T2  - Cellular and Molecular Biology
T1  - Antioxidant status and sex hormones in women with complex endometrial hyperplasia
VL  - 62
IS  - 11
SP  - 51
EP  - 56
DO  - 10.14715/cmb/2016.62.11.9
ER  - 

@article{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Pavlović, Ivan and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2016",
abstract = "Endometrial tissue is under a strong influence of sex hormones. These hormones are considered as developmental factors of endometrial hyperplasia and endometrial cancer. We examined the influence of gonadotropins (follicle-stimulating and luteinizing hormone) and sex hormones (estradiol, progesterone) on oxidant/antioxidant parameters in blood and endometrial tissue of women with complex endometrial hyperplasia. In blood, superoxide dismutase activity was significantly higher in luteal phase and postmenopause compared to the follicular phase. A significant phase-related difference of glutathione peroxidase and glutathione reductase activity was recorded in the endometrium. Both enzymes had lower activity in luteal phase and postmenopause compared to the follicular phase. The linear regression analysis of individual hormonal variables against antioxidant parameters showed negative correlation between glutathione peroxidase activity and gonadotropin concentrations in the endometrium. The regression of hyperplastic to normal endometrium is the purpose of conservative treatment based on administration of progestogens or gonadotropin-releasing hormone analogues. Our findings indicate that gonadotropins influence the antioxidant enzymes activity in women with complex endometrial hyperplasia, which may affect disease development. Further studies are needed to clarify the molecular basis of hormone action on antioxidant system that may potentially initiate a development of treatments based onredox-dependentmechanism.",
journal = "Cellular and Molecular Biology",
title = "Antioxidant status and sex hormones in women with complex endometrial hyperplasia",
volume = "62",
number = "11",
pages = "51-56",
doi = "10.14715/cmb/2016.62.11.9"
}

Pejić, S., Todorović, A., Stojiljković, V., Pavlović, I., Gavrilović, L., Popović, N. M.,& Pajović, S. B.. (2016). Antioxidant status and sex hormones in women with complex endometrial hyperplasia. in Cellular and Molecular Biology, 62(11), 51-56.
https://doi.org/10.14715/cmb/2016.62.11.9

Pejić S, Todorović A, Stojiljković V, Pavlović I, Gavrilović L, Popović NM, Pajović SB. Antioxidant status and sex hormones in women with complex endometrial hyperplasia. in Cellular and Molecular Biology. 2016;62(11):51-56.
doi:10.14715/cmb/2016.62.11.9 .

Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Pavlović, Ivan, Gavrilović, Ljubica, Popović, Nataša M., Pajović, Snežana B., "Antioxidant status and sex hormones in women with complex endometrial hyperplasia" in Cellular and Molecular Biology, 62, no. 11 (2016):51-56,
https://doi.org/10.14715/cmb/2016.62.11.9 . .

TY  - CONF
AU  - Pavlović, Ivan
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Pejić, Snežana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7171
AB  - Renal cell carcinoma is the most common and the most lethal type of kidney cancer with the highest incidence in developed countries. At the moment of diagnosis, many patients already have developed metastases. Tobacco smoking, obesity, hypertension and occupational exposure are established risk factors. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) are antioxidant enzymes (AOE) that have a key role in protecting from reactive oxygen species (ROS) produced in pathological processes. We examined the antioxidant capacity of tumour tissue in patients with clear cell type of renal cell carcinoma. The results showed a significant decrease of AOE activities in the tumour tissue compared to the normal kidney tissue, indicating that the tumour tissue is under permanent oxidative stress. Renal cell carcinoma is highly resistant to radiation therapy, which is also associated with free radical production. Since the tumour tissue has impaired AO capacity, the findings of this study may contribute to the improvement of the potential therapeutic treatments.
C3  - RAD Conference Proceedings
T1  - Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma
SP  - 153
EP  - 155
DO  - 10.21175/RadProc.2016.36
ER  - 

@conference{
author = "Pavlović, Ivan and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B. and Pejić, Snežana",
year = "2016",
abstract = "Renal cell carcinoma is the most common and the most lethal type of kidney cancer with the highest incidence in developed countries. At the moment of diagnosis, many patients already have developed metastases. Tobacco smoking, obesity, hypertension and occupational exposure are established risk factors. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) are antioxidant enzymes (AOE) that have a key role in protecting from reactive oxygen species (ROS) produced in pathological processes. We examined the antioxidant capacity of tumour tissue in patients with clear cell type of renal cell carcinoma. The results showed a significant decrease of AOE activities in the tumour tissue compared to the normal kidney tissue, indicating that the tumour tissue is under permanent oxidative stress. Renal cell carcinoma is highly resistant to radiation therapy, which is also associated with free radical production. Since the tumour tissue has impaired AO capacity, the findings of this study may contribute to the improvement of the potential therapeutic treatments.",
journal = "RAD Conference Proceedings",
title = "Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma",
pages = "153-155",
doi = "10.21175/RadProc.2016.36"
}

Pavlović, I., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M., Pajović, S. B.,& Pejić, S.. (2016). Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma. in RAD Conference Proceedings, 153-155.
https://doi.org/10.21175/RadProc.2016.36

Pavlović I, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Pajović SB, Pejić S. Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma. in RAD Conference Proceedings. 2016;:153-155.
doi:10.21175/RadProc.2016.36 .

Pavlović, Ivan, Todorović, Ana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Pajović, Snežana B., Pejić, Snežana, "Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma" in RAD Conference Proceedings (2016):153-155,
https://doi.org/10.21175/RadProc.2016.36 . .

TY  - CONF
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Dronjak Čučaković, Slađana
AU  - Popović, Nataša
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11403
PB  - Belgrade : Serbian Neurocardiological Society
C3  - NEUROCARD 2015 : 7th Congress of Serbian neuroscience society with international participation, 6th International Symposium on Noninvasive Electrocardiology :  Program and the book of abstracts
T1  - Daily treadmill running maintains the sythesis of catecholamines on the basal level and decreases oxidative stress in the right and left heart auricles of chronically stressed rats
SP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11403
ER  - 

@conference{
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Dronjak Čučaković, Slađana and Popović, Nataša and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pajović, Snežana B.",
year = "2015",
publisher = "Belgrade : Serbian Neurocardiological Society",
journal = "NEUROCARD 2015 : 7th Congress of Serbian neuroscience society with international participation, 6th International Symposium on Noninvasive Electrocardiology :  Program and the book of abstracts",
title = "Daily treadmill running maintains the sythesis of catecholamines on the basal level and decreases oxidative stress in the right and left heart auricles of chronically stressed rats",
pages = "92",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11403"
}

Gavrilović, L., Stojiljković, V., Dronjak Čučaković, S., Popović, N., Pejić, S., Todorović, A., Pavlović, I.,& Pajović, S. B.. (2015). Daily treadmill running maintains the sythesis of catecholamines on the basal level and decreases oxidative stress in the right and left heart auricles of chronically stressed rats. in NEUROCARD 2015 : 7th Congress of Serbian neuroscience society with international participation, 6th International Symposium on Noninvasive Electrocardiology :  Program and the book of abstracts
Belgrade : Serbian Neurocardiological Society., 92.
https://hdl.handle.net/21.15107/rcub_vinar_11403

Gavrilović L, Stojiljković V, Dronjak Čučaković S, Popović N, Pejić S, Todorović A, Pavlović I, Pajović SB. Daily treadmill running maintains the sythesis of catecholamines on the basal level and decreases oxidative stress in the right and left heart auricles of chronically stressed rats. in NEUROCARD 2015 : 7th Congress of Serbian neuroscience society with international participation, 6th International Symposium on Noninvasive Electrocardiology :  Program and the book of abstracts. 2015;:92.
https://hdl.handle.net/21.15107/rcub_vinar_11403 .

Gavrilović, Ljubica, Stojiljković, Vesna, Dronjak Čučaković, Slađana, Popović, Nataša, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pajović, Snežana B., "Daily treadmill running maintains the sythesis of catecholamines on the basal level and decreases oxidative stress in the right and left heart auricles of chronically stressed rats" in NEUROCARD 2015 : 7th Congress of Serbian neuroscience society with international participation, 6th International Symposium on Noninvasive Electrocardiology :  Program and the book of abstracts (2015):92,
https://hdl.handle.net/21.15107/rcub_vinar_11403 .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/498
AB  - Cancer of the reproductive tract is an important cause of morbidity and mortality among women worldwide. In this study we evaluated the influence of diagnostic categories, age and reproductive factors on antioxidant enzymes and lipid hydroperoxides in the blood of gynaecological patients diagnosed with endometrial polyp, myoma, hyperplasia simplex, hyperplasia complex and endometrial adenocarcinoma. Multivariate regression analysis was used to assess the association of diagnosis, age, parity, abortions and abnormal uterine bleeding with the examined parameters. Diagnosis provided the best predictive model for superoxide dismutase, catalase and glutathione peroxidase activities, and also for the lipid hydroperoxide level. Abortions fitted the best predictive model for superoxide dismutase activity. A significant correlation was also found between the predictor variables themselves. This study showed that reproductive and other factors may be associated, at least partially, with antioxidant capacity and ability to defend against the oxidative damage in gynaecological patients with various diagnoses.
T2  - Folia Biologica
T1  - Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors
VL  - 61
IS  - 1
SP  - 26
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_vinar_498
ER  - 

@article{
author = "Pejić, Snežana and Stojiljković, Vesna and Todorović, Ana and Gavrilović, Ljubica and Popović, Nataša M. and Pavlović, Ivan and Pajović, Snežana B.",
year = "2015",
abstract = "Cancer of the reproductive tract is an important cause of morbidity and mortality among women worldwide. In this study we evaluated the influence of diagnostic categories, age and reproductive factors on antioxidant enzymes and lipid hydroperoxides in the blood of gynaecological patients diagnosed with endometrial polyp, myoma, hyperplasia simplex, hyperplasia complex and endometrial adenocarcinoma. Multivariate regression analysis was used to assess the association of diagnosis, age, parity, abortions and abnormal uterine bleeding with the examined parameters. Diagnosis provided the best predictive model for superoxide dismutase, catalase and glutathione peroxidase activities, and also for the lipid hydroperoxide level. Abortions fitted the best predictive model for superoxide dismutase activity. A significant correlation was also found between the predictor variables themselves. This study showed that reproductive and other factors may be associated, at least partially, with antioxidant capacity and ability to defend against the oxidative damage in gynaecological patients with various diagnoses.",
journal = "Folia Biologica",
title = "Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors",
volume = "61",
number = "1",
pages = "26-32",
url = "https://hdl.handle.net/21.15107/rcub_vinar_498"
}

Pejić, S., Stojiljković, V., Todorović, A., Gavrilović, L., Popović, N. M., Pavlović, I.,& Pajović, S. B.. (2015). Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors. in Folia Biologica, 61(1), 26-32.
https://hdl.handle.net/21.15107/rcub_vinar_498

Pejić S, Stojiljković V, Todorović A, Gavrilović L, Popović NM, Pavlović I, Pajović SB. Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors. in Folia Biologica. 2015;61(1):26-32.
https://hdl.handle.net/21.15107/rcub_vinar_498 .

Pejić, Snežana, Stojiljković, Vesna, Todorović, Ana, Gavrilović, Ljubica, Popović, Nataša M., Pavlović, Ivan, Pajović, Snežana B., "Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors" in Folia Biologica, 61, no. 1 (2015):26-32,
https://hdl.handle.net/21.15107/rcub_vinar_498 .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/751
AB  - Uterine leiomyomas are benign soft-tissues tumors that arise from uterine smooth muscle tissue. Etiopathogenesis of leiomyomas is not well understood. We aimed to examine whether antioxidant enzyme activities and lipid hydroperoxides level in patients with leiomyoma are influenced by changes in sex hormones and gonadotropins (estradiol (E2), progesterone, FSH, and LH) during menstrual cycle and in postmenopause. The material consisted of blood and uterine tissue specimens. Hormone concentrations were determined and assays for superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities and lipid hydroperoxides concentration were performed. In blood of examined women, a significant difference in catalase, glutathione peroxidase and glutathione reductase activity was recorded among the phases. There was also a positive correlation between the estradiol/progesterone concentration and the catalase activity. Progesterone negatively correlated with lipid hydroperoxides level. In myoma tissue, we recorded a phase-related difference in lipid hydroperoxides level and activities of superoxide dismutase, glutathione peroxidase activities, and glutathione reductase. Negative correlation was observed between FSH and glutathione peroxidase. The results suggest that antioxidant status in patients with uterine leiomyoma is influenced by the changes in sex hormones during the menstrual cycle and in postmenopause, indicating a role of the observed relationship in the leiomyoma etiology.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Antioxidant status in women with uterine leiomyoma: relation with sex hormones
VL  - 87
IS  - 3
SP  - 1771
EP  - 1782
DO  - 10.1590/0001-3765201520130416
ER  - 

@article{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2015",
abstract = "Uterine leiomyomas are benign soft-tissues tumors that arise from uterine smooth muscle tissue. Etiopathogenesis of leiomyomas is not well understood. We aimed to examine whether antioxidant enzyme activities and lipid hydroperoxides level in patients with leiomyoma are influenced by changes in sex hormones and gonadotropins (estradiol (E2), progesterone, FSH, and LH) during menstrual cycle and in postmenopause. The material consisted of blood and uterine tissue specimens. Hormone concentrations were determined and assays for superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities and lipid hydroperoxides concentration were performed. In blood of examined women, a significant difference in catalase, glutathione peroxidase and glutathione reductase activity was recorded among the phases. There was also a positive correlation between the estradiol/progesterone concentration and the catalase activity. Progesterone negatively correlated with lipid hydroperoxides level. In myoma tissue, we recorded a phase-related difference in lipid hydroperoxides level and activities of superoxide dismutase, glutathione peroxidase activities, and glutathione reductase. Negative correlation was observed between FSH and glutathione peroxidase. The results suggest that antioxidant status in patients with uterine leiomyoma is influenced by the changes in sex hormones during the menstrual cycle and in postmenopause, indicating a role of the observed relationship in the leiomyoma etiology.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Antioxidant status in women with uterine leiomyoma: relation with sex hormones",
volume = "87",
number = "3",
pages = "1771-1782",
doi = "10.1590/0001-3765201520130416"
}

Pejić, S., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M.,& Pajović, S. B.. (2015). Antioxidant status in women with uterine leiomyoma: relation with sex hormones. in Anais de Academia Brasileira de Ciencias, 87(3), 1771-1782.
https://doi.org/10.1590/0001-3765201520130416

Pejić S, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Pajović SB. Antioxidant status in women with uterine leiomyoma: relation with sex hormones. in Anais de Academia Brasileira de Ciencias. 2015;87(3):1771-1782.
doi:10.1590/0001-3765201520130416 .

Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Pajović, Snežana B., "Antioxidant status in women with uterine leiomyoma: relation with sex hormones" in Anais de Academia Brasileira de Ciencias, 87, no. 3 (2015):1771-1782,
https://doi.org/10.1590/0001-3765201520130416 . .

TY  - JOUR
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Saičić, Zorica S.
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/815
AB  - Purpose Previously, we examined manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT) activities in rat brain irradiated with 2 or 3 Gy of gamma-rays. The results indicated that lower MnSOD activity and inducibility found in hippocampus might explain higher radiosensitivity of this brain region. Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD, and CAT activities after dose of 5 Gy and to find out if differences in MnSOD activity are caused by changes in its expression. Heads of 4-day-old female rats were irradiated with gamma-rays, using Co-60. Animals were sacrificed 1/24 h after exposure. Hippocampus and cortex tissues were prepared for enzyme activity measurements and Western blot analysis. One hour after exposure, gamma-rays significantly decreased MnSOD activity in both examined brain regions. Twenty-four hours later, MnSOD recovery showed dose and regional dependence. It was weaker at higher doses and in hippocampal region. MnSOD expression changed in the similar manner as MnSOD activity only at lower doses of gamma-rays. In both examined brain regions, gamma radiation significantly decreased CuZnSOD activity and did not change activity of CAT. Our results confirmed that MnSOD plays an important role in different regional radiosensitivity but also showed that depending on dose, radiation affects MnSOD level by utterly different mechanisms. Postradiation changes of CuZnSOD and CAT are not regionally specific and therefore, cannot account for the different radiosensitivity of the hippocampus and cortex.
PB  - Springer Nature
T2  - Childs Nervous System
T1  - Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity
VL  - 31
IS  - 12
SP  - 2249
EP  - 2256
DO  - 10.1007/s00381-015-2807-2
ER  - 

@article{
author = "Todorović, Ana and Pejić, Snežana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pavlović, Ivan and Saičić, Zorica S. and Pajović, Snežana B.",
year = "2015",
abstract = "Purpose Previously, we examined manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT) activities in rat brain irradiated with 2 or 3 Gy of gamma-rays. The results indicated that lower MnSOD activity and inducibility found in hippocampus might explain higher radiosensitivity of this brain region. Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD, and CAT activities after dose of 5 Gy and to find out if differences in MnSOD activity are caused by changes in its expression. Heads of 4-day-old female rats were irradiated with gamma-rays, using Co-60. Animals were sacrificed 1/24 h after exposure. Hippocampus and cortex tissues were prepared for enzyme activity measurements and Western blot analysis. One hour after exposure, gamma-rays significantly decreased MnSOD activity in both examined brain regions. Twenty-four hours later, MnSOD recovery showed dose and regional dependence. It was weaker at higher doses and in hippocampal region. MnSOD expression changed in the similar manner as MnSOD activity only at lower doses of gamma-rays. In both examined brain regions, gamma radiation significantly decreased CuZnSOD activity and did not change activity of CAT. Our results confirmed that MnSOD plays an important role in different regional radiosensitivity but also showed that depending on dose, radiation affects MnSOD level by utterly different mechanisms. Postradiation changes of CuZnSOD and CAT are not regionally specific and therefore, cannot account for the different radiosensitivity of the hippocampus and cortex.",
publisher = "Springer Nature",
journal = "Childs Nervous System",
title = "Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity",
volume = "31",
number = "12",
pages = "2249-2256",
doi = "10.1007/s00381-015-2807-2"
}

Todorović, A., Pejić, S., Stojiljković, V., Gavrilović, L., Popović, N. M., Pavlović, I., Saičić, Z. S.,& Pajović, S. B.. (2015). Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity. in Childs Nervous System
Springer Nature., 31(12), 2249-2256.
https://doi.org/10.1007/s00381-015-2807-2

Todorović A, Pejić S, Stojiljković V, Gavrilović L, Popović NM, Pavlović I, Saičić ZS, Pajović SB. Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity. in Childs Nervous System. 2015;31(12):2249-2256.
doi:10.1007/s00381-015-2807-2 .

Todorović, Ana, Pejić, Snežana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Pavlović, Ivan, Saičić, Zorica S., Pajović, Snežana B., "Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity" in Childs Nervous System, 31, no. 12 (2015):2249-2256,
https://doi.org/10.1007/s00381-015-2807-2 . .

TY  - CONF
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7131
AB  - Endometrial hyperplasia complex is gynecological disorder characterized by morphological irregularities of glands shape and size. Antioxidant enzymes (AOE), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), have an essential role in preventing oxidative damage in cell caused by reactive oxygen species (ROS). In this study, we examined the AO status in hyperplastic tissue of patients in menstrual cycle (follicular and luteal phase) and in postmenopause, as well as the relationship between sex hormones and AO parameters. The phase-related activity of GPx and GR in examined patients was significantly different than in healthy women. A significant negative correlation between FSH/LH level and GPx activiy was observed. Endometrial hyperplasias are considered as precancerous lesions and are treated either conservatively or surgicaly, and also by radiation therapy. Since the effects of these therapies are associated with AO and hormonal changes, our results may contribute to the prediction of potential therapeutic efficacy and to selection of the most effective treatment for hyperplasia complex.
C3  - RAD Conference Proceedings
T1  - Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones
SP  - 467
EP  - 470
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7131
ER  - 

@conference{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pavlović, Ivan and Pajović, Snežana B.",
year = "2015",
abstract = "Endometrial hyperplasia complex is gynecological disorder characterized by morphological irregularities of glands shape and size. Antioxidant enzymes (AOE), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), have an essential role in preventing oxidative damage in cell caused by reactive oxygen species (ROS). In this study, we examined the AO status in hyperplastic tissue of patients in menstrual cycle (follicular and luteal phase) and in postmenopause, as well as the relationship between sex hormones and AO parameters. The phase-related activity of GPx and GR in examined patients was significantly different than in healthy women. A significant negative correlation between FSH/LH level and GPx activiy was observed. Endometrial hyperplasias are considered as precancerous lesions and are treated either conservatively or surgicaly, and also by radiation therapy. Since the effects of these therapies are associated with AO and hormonal changes, our results may contribute to the prediction of potential therapeutic efficacy and to selection of the most effective treatment for hyperplasia complex.",
journal = "RAD Conference Proceedings",
title = "Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones",
pages = "467-470",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7131"
}

Pejić, S., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M., Pavlović, I.,& Pajović, S. B.. (2015). Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones. in RAD Conference Proceedings, 467-470.
https://hdl.handle.net/21.15107/rcub_vinar_7131

Pejić S, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Pavlović I, Pajović SB. Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones. in RAD Conference Proceedings. 2015;:467-470.
https://hdl.handle.net/21.15107/rcub_vinar_7131 .

Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Pavlović, Ivan, Pajović, Snežana B., "Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones" in RAD Conference Proceedings (2015):467-470,
https://hdl.handle.net/21.15107/rcub_vinar_7131 .

TY  - CHAP
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8408
AB  - Cancer of the reproductive tract is an important source of morbidity and mortality among women worldwide. Factors affecting endometrial cancer and endometrial hyperplasia are known to be similar. Endometrial hyperplasia is abnormal proliferation of the glands and the stroma resulting in architectural and cytological modifications. Due to hormonal changes, this condition is most common among women who are nearing the menopause or have reached the menopause. Antioxidant system has a role in preventing cancer initiation and promotion. Since the carcinogenesis occurs in several stages, it is likely that the antioxidant defense depends on the type of cell and tissue. The objective of this study was to investigate whether antioxidant enzymes activities and lipid hydroperoxides concentration in patients with endometrial hyperplasia are influenced by the changes in sex hormones level (estradiol, progesterone, FSH, and LH) during the menstrual cycle and in postmenopause. The material we used consisted of blood and endometrial tissue specimens of women diagnosed with endometrial hyperplasia simplex. Patients were divided in groups depending on the phase of the menstrual cycle: follicular phase, luteal phase and postmenopause. The activities of antioxidant enzymes and the lipid hydroperoxides level were compared among the phases to test the differences and a linear regression model was used to evaluate the associations between hormone levels and antioxidant/oxidant variables. In the blood of examined patients, we observed a phase-related changes of LOOH concentrations. Significant negative correlation between FSH concentration and GR activity (r= -0.42, p<0.05) and significant positive correlation between LH and LOOH concentrations (r= 0.038, p<0.05) was found. In hyperplasia simplex tissue we recorded significant phase-related changes of LOOH level as well as of AO enzyme activities. SOD and CAT had similar activity pattern, which was higher in luteal phase and in postmenopause, compared to follicular phase (p<0.05). GPx and GR activities did not show any statistical difference. Also, negative correlation between progesterone and GR activity (r=-0.036, p<0.05) was observed. Hormonal influence on AO system is of importance in gynecological diseases etiology since they may promote cell proliferation but are also used in conservative therapy, especially for hyperplasia simplex. However, the role of ROS production as a risk factor for endometrial hyperplasia still needs to be clarified as well as the role of AO status in response to gonadotropins and sex steroids.
T2  - Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder
T1  - Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia
IS  - Ch. 9
SP  - 243
EP  - 279
DO  - 10.5772/60853
ER  - 

@inbook{
editor = "Gowder, Sivakumar Joghi Thatha",
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Pavlović, Ivan and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2015",
abstract = "Cancer of the reproductive tract is an important source of morbidity and mortality among women worldwide. Factors affecting endometrial cancer and endometrial hyperplasia are known to be similar. Endometrial hyperplasia is abnormal proliferation of the glands and the stroma resulting in architectural and cytological modifications. Due to hormonal changes, this condition is most common among women who are nearing the menopause or have reached the menopause. Antioxidant system has a role in preventing cancer initiation and promotion. Since the carcinogenesis occurs in several stages, it is likely that the antioxidant defense depends on the type of cell and tissue. The objective of this study was to investigate whether antioxidant enzymes activities and lipid hydroperoxides concentration in patients with endometrial hyperplasia are influenced by the changes in sex hormones level (estradiol, progesterone, FSH, and LH) during the menstrual cycle and in postmenopause. The material we used consisted of blood and endometrial tissue specimens of women diagnosed with endometrial hyperplasia simplex. Patients were divided in groups depending on the phase of the menstrual cycle: follicular phase, luteal phase and postmenopause. The activities of antioxidant enzymes and the lipid hydroperoxides level were compared among the phases to test the differences and a linear regression model was used to evaluate the associations between hormone levels and antioxidant/oxidant variables. In the blood of examined patients, we observed a phase-related changes of LOOH concentrations. Significant negative correlation between FSH concentration and GR activity (r= -0.42, p<0.05) and significant positive correlation between LH and LOOH concentrations (r= 0.038, p<0.05) was found. In hyperplasia simplex tissue we recorded significant phase-related changes of LOOH level as well as of AO enzyme activities. SOD and CAT had similar activity pattern, which was higher in luteal phase and in postmenopause, compared to follicular phase (p<0.05). GPx and GR activities did not show any statistical difference. Also, negative correlation between progesterone and GR activity (r=-0.036, p<0.05) was observed. Hormonal influence on AO system is of importance in gynecological diseases etiology since they may promote cell proliferation but are also used in conservative therapy, especially for hyperplasia simplex. However, the role of ROS production as a risk factor for endometrial hyperplasia still needs to be clarified as well as the role of AO status in response to gonadotropins and sex steroids.",
journal = "Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder",
booktitle = "Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia",
number = "Ch. 9",
pages = "243-279",
doi = "10.5772/60853"
}

Gowder, S. J. T., Pejić, S., Todorović, A., Stojiljković, V., Pavlović, I., Gavrilović, L., Popović, N. M.,& Pajović, S. B.. (2015). Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia. in Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder(Ch. 9), 243-279.
https://doi.org/10.5772/60853

Gowder SJT, Pejić S, Todorović A, Stojiljković V, Pavlović I, Gavrilović L, Popović NM, Pajović SB. Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia. in Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder. 2015;(Ch. 9):243-279.
doi:10.5772/60853 .

Gowder, Sivakumar Joghi Thatha, Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Pavlović, Ivan, Gavrilović, Ljubica, Popović, Nataša M., Pajović, Snežana B., "Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia" in Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder, no. Ch. 9 (2015):243-279,
https://doi.org/10.5772/60853 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Popović, Nataša M.
AU  - Korićanac, Lela
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5447
AB  - Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.
T2  - Archives of Medical Science
T1  - Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons
VL  - 10
IS  - 3
SP  - 578
EP  - 586
DO  - 10.5114/aoms.2014.43751
ER  - 

@article{
author = "Keta, Otilija D. and Todorović, Danijela V. and Popović, Nataša M. and Korićanac, Lela and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2014",
abstract = "Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.",
journal = "Archives of Medical Science",
title = "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons",
volume = "10",
number = "3",
pages = "578-586",
doi = "10.5114/aoms.2014.43751"
}

Keta, O. D., Todorović, D. V., Popović, N. M., Korićanac, L., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2014). Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. in Archives of Medical Science, 10(3), 578-586.
https://doi.org/10.5114/aoms.2014.43751

Keta OD, Todorović DV, Popović NM, Korićanac L, Cuttone G, Petrović IM, Ristić-Fira A. Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. in Archives of Medical Science. 2014;10(3):578-586.
doi:10.5114/aoms.2014.43751 .

Keta, Otilija D., Todorović, Danijela V., Popović, Nataša M., Korićanac, Lela, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons" in Archives of Medical Science, 10, no. 3 (2014):578-586,
https://doi.org/10.5114/aoms.2014.43751 . .