TY  - JOUR
AU  - Barać, Milena
AU  - Petrović, Milan
AU  - Petrović, Nina
AU  - Nikolić-Jakoba, Nataša
AU  - Aleksić, Zoran
AU  - Todorović, Lidija
AU  - Petrović-Stanojević, Nataša
AU  - Anđelić-Jelić, Marina
AU  - Davidović, Aleksandar
AU  - Milašin, Jelena
AU  - Roganović, Jelena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11499
AB  - Type 2 diabetes mellitus (T2DM) is associated with functional deterioration of the salivary gland and dental pulp, related to oxidative stress. The aim was to integrate experimental and bioinformatic findings to analyze the cellular mechanism of melatonin (MEL) action in the human parotid gland and dental pulp in diabetes. Human parotid gland tissue was obtained from 16 non-diabetic and 16 diabetic participants, as well as human dental pulp from 15 non-diabetic and 15 diabetic participants. In human non-diabetic and diabetic parotid gland cells (hPGCs) as well as in dental pulp cells (hDPCs), cultured in hyper- and normoglycemic conditions, glial cell linederived neurotrophic factor (GDNF), MEL, inducible nitric oxide synthase (iNOS) protein expression, and superoxide dismutase (SOD) activity were measured by enzyme-linked immunosorbent assay (ELISA) and spectrophotometrically. Bioinformatic analysis was performed using ShinyGO (v.0.75) application. Diabetic participants had increased GDNF and decreased MEL in parotid (p < 0.01) and dental pulp (p < 0.05) tissues, associated with increased iNOS and SOD activity. Normoglycemic hDPCs and non-diabetic hPGCs treated with 0.1 mM MEL had increased GDNF (p < 0.05), while hyperglycemic hDPCs treated with 1 mM MEL showed a decrease in up-regulated GDNF (p < 0.05). Enrichment analyses showed interference with stress and ATF/CREB signaling. MEL induced the stress-protective mechanism in hyperglycemic hDPCs and diabetic hPGCs, suggesting MEL could be beneficial for diabetes-associated disturbances in oral tissues.
T2  - International Journal of Environmental Research and Public Health
T1  - Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings
VL  - 20
IS  - 18
SP  - 6727
DO  - 10.3390/ijerph20186727
ER  - 

@article{
author = "Barać, Milena and Petrović, Milan and Petrović, Nina and Nikolić-Jakoba, Nataša and Aleksić, Zoran and Todorović, Lidija and Petrović-Stanojević, Nataša and Anđelić-Jelić, Marina and Davidović, Aleksandar and Milašin, Jelena and Roganović, Jelena",
year = "2023",
abstract = "Type 2 diabetes mellitus (T2DM) is associated with functional deterioration of the salivary gland and dental pulp, related to oxidative stress. The aim was to integrate experimental and bioinformatic findings to analyze the cellular mechanism of melatonin (MEL) action in the human parotid gland and dental pulp in diabetes. Human parotid gland tissue was obtained from 16 non-diabetic and 16 diabetic participants, as well as human dental pulp from 15 non-diabetic and 15 diabetic participants. In human non-diabetic and diabetic parotid gland cells (hPGCs) as well as in dental pulp cells (hDPCs), cultured in hyper- and normoglycemic conditions, glial cell linederived neurotrophic factor (GDNF), MEL, inducible nitric oxide synthase (iNOS) protein expression, and superoxide dismutase (SOD) activity were measured by enzyme-linked immunosorbent assay (ELISA) and spectrophotometrically. Bioinformatic analysis was performed using ShinyGO (v.0.75) application. Diabetic participants had increased GDNF and decreased MEL in parotid (p < 0.01) and dental pulp (p < 0.05) tissues, associated with increased iNOS and SOD activity. Normoglycemic hDPCs and non-diabetic hPGCs treated with 0.1 mM MEL had increased GDNF (p < 0.05), while hyperglycemic hDPCs treated with 1 mM MEL showed a decrease in up-regulated GDNF (p < 0.05). Enrichment analyses showed interference with stress and ATF/CREB signaling. MEL induced the stress-protective mechanism in hyperglycemic hDPCs and diabetic hPGCs, suggesting MEL could be beneficial for diabetes-associated disturbances in oral tissues.",
journal = "International Journal of Environmental Research and Public Health",
title = "Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings",
volume = "20",
number = "18",
pages = "6727",
doi = "10.3390/ijerph20186727"
}

Barać, M., Petrović, M., Petrović, N., Nikolić-Jakoba, N., Aleksić, Z., Todorović, L., Petrović-Stanojević, N., Anđelić-Jelić, M., Davidović, A., Milašin, J.,& Roganović, J.. (2023). Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings. in International Journal of Environmental Research and Public Health, 20(18), 6727.
https://doi.org/10.3390/ijerph20186727

Barać M, Petrović M, Petrović N, Nikolić-Jakoba N, Aleksić Z, Todorović L, Petrović-Stanojević N, Anđelić-Jelić M, Davidović A, Milašin J, Roganović J. Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings. in International Journal of Environmental Research and Public Health. 2023;20(18):6727.
doi:10.3390/ijerph20186727 .

Barać, Milena, Petrović, Milan, Petrović, Nina, Nikolić-Jakoba, Nataša, Aleksić, Zoran, Todorović, Lidija, Petrović-Stanojević, Nataša, Anđelić-Jelić, Marina, Davidović, Aleksandar, Milašin, Jelena, Roganović, Jelena, "Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings" in International Journal of Environmental Research and Public Health, 20, no. 18 (2023):6727,
https://doi.org/10.3390/ijerph20186727 . .

TY  - JOUR
AU  - Radović, Nikola
AU  - Nikolić Jakoba, Nataša
AU  - Petrović, Nina
AU  - Milosavljević, Aleksandra
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jcpe.12888
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7753
AB  - Aim: Recent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.
T2  - Journal of Clinical Periodontology
T1  - MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients
VL  - 45
IS  - 6
SP  - 663
EP  - 671
DO  - 10.1111/jcpe.12888
ER  - 

@article{
author = "Radović, Nikola and Nikolić Jakoba, Nataša and Petrović, Nina and Milosavljević, Aleksandra and Brković, Božidar and Roganović, Jelena",
year = "2018",
abstract = "Aim: Recent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.",
journal = "Journal of Clinical Periodontology",
title = "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients",
volume = "45",
number = "6",
pages = "663-671",
doi = "10.1111/jcpe.12888"
}

Radović, N., Nikolić Jakoba, N., Petrović, N., Milosavljević, A., Brković, B.,& Roganović, J.. (2018). MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology, 45(6), 663-671.
https://doi.org/10.1111/jcpe.12888

Radović N, Nikolić Jakoba N, Petrović N, Milosavljević A, Brković B, Roganović J. MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology. 2018;45(6):663-671.
doi:10.1111/jcpe.12888 .

Radović, Nikola, Nikolić Jakoba, Nataša, Petrović, Nina, Milosavljević, Aleksandra, Brković, Božidar, Roganović, Jelena, "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients" in Journal of Clinical Periodontology, 45, no. 6 (2018):663-671,
https://doi.org/10.1111/jcpe.12888 . .