TY  - JOUR
AU  - Fenech, M
AU  - Bonassi, S
AU  - Turner, J
AU  - Lando, C
AU  - Ceppi, M
AU  - Chang, WP
AU  - Holland, N
AU  - Kirsch-Volders, M
AU  - Zeiger, E
AU  - Bigatti, MP
AU  - Bolognesi, C
AU  - Cao, J
AU  - De Luca, G
AU  - Di Giorgio, M
AU  - Ferguson, LR
AU  - Fucic, A
AU  - Lima, OG
AU  - Hadjidekova, VV
AU  - Hrelia, P
AU  - Jaworska, A
AU  - Joksić, Gordana
AU  - Krishnaja, AP
AU  - Lee, TK
AU  - Martelli, A
AU  - McKay, MJ
AU  - Migliore, L
AU  - Mirkova, E
AU  - Muller, WU
AU  - Odagiri, Y
AU  - Orsiere, T
AU  - Scarfi, MR
AU  - Silva, MJ
AU  - Sofuni, T
AU  - Surralles, J
AU  - Trenta, G
AU  - Vorobtsova, I
AU  - Vral, A
AU  - Zijno, A
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2647
T2  - Mutation Research: Genetic Toxicology and Environmental Mutagenesis
T1  - Erratum: Intra and inter-laboratory variation in the scoring of micronucici and nucleoplasmic bridges in binucleated human lymphocytes - Results of an international slide-scoring exercise by the HUMN project (vol 534, pg 45, 2002)
VL  - 538
IS  - 1-2
SP  - 185
EP  - 186
DO  - 10.1016/S1383-5718(03)00088-3
ER  - 

@article{
author = "Fenech, M and Bonassi, S and Turner, J and Lando, C and Ceppi, M and Chang, WP and Holland, N and Kirsch-Volders, M and Zeiger, E and Bigatti, MP and Bolognesi, C and Cao, J and De Luca, G and Di Giorgio, M and Ferguson, LR and Fucic, A and Lima, OG and Hadjidekova, VV and Hrelia, P and Jaworska, A and Joksić, Gordana and Krishnaja, AP and Lee, TK and Martelli, A and McKay, MJ and Migliore, L and Mirkova, E and Muller, WU and Odagiri, Y and Orsiere, T and Scarfi, MR and Silva, MJ and Sofuni, T and Surralles, J and Trenta, G and Vorobtsova, I and Vral, A and Zijno, A",
year = "2003",
journal = "Mutation Research: Genetic Toxicology and Environmental Mutagenesis",
title = "Erratum: Intra and inter-laboratory variation in the scoring of micronucici and nucleoplasmic bridges in binucleated human lymphocytes - Results of an international slide-scoring exercise by the HUMN project (vol 534, pg 45, 2002)",
volume = "538",
number = "1-2",
pages = "185-186",
doi = "10.1016/S1383-5718(03)00088-3"
}

Fenech, M., Bonassi, S., Turner, J., Lando, C., Ceppi, M., Chang, W., Holland, N., Kirsch-Volders, M., Zeiger, E., Bigatti, M., Bolognesi, C., Cao, J., De Luca, G., Di Giorgio, M., Ferguson, L., Fucic, A., Lima, O., Hadjidekova, V., Hrelia, P., Jaworska, A., Joksić, G., Krishnaja, A., Lee, T., Martelli, A., McKay, M., Migliore, L., Mirkova, E., Muller, W., Odagiri, Y., Orsiere, T., Scarfi, M., Silva, M., Sofuni, T., Surralles, J., Trenta, G., Vorobtsova, I., Vral, A.,& Zijno, A.. (2003). Erratum: Intra and inter-laboratory variation in the scoring of micronucici and nucleoplasmic bridges in binucleated human lymphocytes - Results of an international slide-scoring exercise by the HUMN project (vol 534, pg 45, 2002). in Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 538(1-2), 185-186.
https://doi.org/10.1016/S1383-5718(03)00088-3

Fenech M, Bonassi S, Turner J, Lando C, Ceppi M, Chang W, Holland N, Kirsch-Volders M, Zeiger E, Bigatti M, Bolognesi C, Cao J, De Luca G, Di Giorgio M, Ferguson L, Fucic A, Lima O, Hadjidekova V, Hrelia P, Jaworska A, Joksić G, Krishnaja A, Lee T, Martelli A, McKay M, Migliore L, Mirkova E, Muller W, Odagiri Y, Orsiere T, Scarfi M, Silva M, Sofuni T, Surralles J, Trenta G, Vorobtsova I, Vral A, Zijno A. Erratum: Intra and inter-laboratory variation in the scoring of micronucici and nucleoplasmic bridges in binucleated human lymphocytes - Results of an international slide-scoring exercise by the HUMN project (vol 534, pg 45, 2002). in Mutation Research: Genetic Toxicology and Environmental Mutagenesis. 2003;538(1-2):185-186.
doi:10.1016/S1383-5718(03)00088-3 .

Fenech, M, Bonassi, S, Turner, J, Lando, C, Ceppi, M, Chang, WP, Holland, N, Kirsch-Volders, M, Zeiger, E, Bigatti, MP, Bolognesi, C, Cao, J, De Luca, G, Di Giorgio, M, Ferguson, LR, Fucic, A, Lima, OG, Hadjidekova, VV, Hrelia, P, Jaworska, A, Joksić, Gordana, Krishnaja, AP, Lee, TK, Martelli, A, McKay, MJ, Migliore, L, Mirkova, E, Muller, WU, Odagiri, Y, Orsiere, T, Scarfi, MR, Silva, MJ, Sofuni, T, Surralles, J, Trenta, G, Vorobtsova, I, Vral, A, Zijno, A, "Erratum: Intra and inter-laboratory variation in the scoring of micronucici and nucleoplasmic bridges in binucleated human lymphocytes - Results of an international slide-scoring exercise by the HUMN project (vol 534, pg 45, 2002)" in Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 538, no. 1-2 (2003):185-186,
https://doi.org/10.1016/S1383-5718(03)00088-3 . .

TY  - JOUR
AU  - Fenech, M
AU  - Bonassi, S
AU  - Turner, J
AU  - Lando, C
AU  - Ceppi, M
AU  - Chang, WP
AU  - Holland, N
AU  - Kirsch-Volders, M
AU  - Zeiger, E
AU  - Bigatti, MP
AU  - Bolognesi, C
AU  - Cao, J
AU  - De Luca, G
AU  - Di Giorgio, M
AU  - Ferguson, LR
AU  - Fucic, A
AU  - Lima, OG
AU  - Hadjidekova, VV
AU  - Hrelia, P
AU  - Jaworska, A
AU  - Joksić, Gordana
AU  - Krishnaja, AP
AU  - Lee, TK
AU  - Martelli, A
AU  - McKay, MJ
AU  - Migliore, L
AU  - Mirkova, E
AU  - Muller, WU
AU  - Odagiri, Y
AU  - Orsiere, T
AU  - Scarfi, MR
AU  - Silva, MJ
AU  - Sofuni, T
AU  - Suralles, J
AU  - Trenta, G
AU  - Vorobtsova, I
AU  - Vral, A
AU  - Zijno, A
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2603
AB  - One of the objectives of the HUman MicroNucleus (HUMN) project is to identify the methodological variables that have an important impact on micronucleus (MN) or micronucleated (MNed) cell frequencies measured in human lymphocytes using the cytokinesis-block micronucleus assay, In a previous study we had shown that the scoring criteria used were likely to be an important variable. To determine the extent of residual variation when laboratories scored cells from the same cultures using the same set of standard scoring criteria, an inter-laboratory slide-scoring exercise was performed among 34 laboratories from 21 countries with a total of 51 slide scorers involved. The results of this StUdy show that even under these optimized conditions there is a great variation in the MN frequency or MNed cell frequency obtained by individual laboratories and scorers. All laboratories ranked correctly the MNed cell frequency in cells from cultures that were unirradiated, or exposed to 1 or 2 Gy of gamma rays. The study also estimated that the intra-scorer median coefficient of variation for duplicate MNed cell frequency scores is 29% for unexposed cultures and 14 and 11% for cells exposed to I and 2 Gy, respectively, These values can be used as a standard for quality or acceptability of data in future studies. Using a Poisson regression model it was estimated that radiation dose explained 67% of the variance, while staining method. cell sample, laboratory, and covariance explained 0.6, 0.3, 6.5. and 25.6% of the variance, respectively, leaving only 3.1% of the variance unexplained. As part of this exercise. nucleoplasmic bridges were also estimated by the laboratories however, inexperience in the use of this biomarker of chromosome rearrangement was reflected in the much greater heterogeneity in the data and the unexplained variation estimated by the Poisson model. The results of these studies indicate clearly that even after standardizing culture and scoring conditions it will be necessary to calibrate scorers and laboratories if MN, MNed cell and nucleoplasmic bridge frequencies are to be reliably compared among laboratories and among populations. (C) 2002 Elsevier Science B.V. All rights reserved.
T2  - Mutation Research: Genetic Toxicology and Environmental Mutagenesis
T1  - Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes Results of an international slide-scoring exercise by the HUMN project
VL  - 534
IS  - 1-2
SP  - 45
EP  - 64
DO  - 10.1016/S1383-5718(02)00248-6
ER  - 

@article{
author = "Fenech, M and Bonassi, S and Turner, J and Lando, C and Ceppi, M and Chang, WP and Holland, N and Kirsch-Volders, M and Zeiger, E and Bigatti, MP and Bolognesi, C and Cao, J and De Luca, G and Di Giorgio, M and Ferguson, LR and Fucic, A and Lima, OG and Hadjidekova, VV and Hrelia, P and Jaworska, A and Joksić, Gordana and Krishnaja, AP and Lee, TK and Martelli, A and McKay, MJ and Migliore, L and Mirkova, E and Muller, WU and Odagiri, Y and Orsiere, T and Scarfi, MR and Silva, MJ and Sofuni, T and Suralles, J and Trenta, G and Vorobtsova, I and Vral, A and Zijno, A",
year = "2003",
abstract = "One of the objectives of the HUman MicroNucleus (HUMN) project is to identify the methodological variables that have an important impact on micronucleus (MN) or micronucleated (MNed) cell frequencies measured in human lymphocytes using the cytokinesis-block micronucleus assay, In a previous study we had shown that the scoring criteria used were likely to be an important variable. To determine the extent of residual variation when laboratories scored cells from the same cultures using the same set of standard scoring criteria, an inter-laboratory slide-scoring exercise was performed among 34 laboratories from 21 countries with a total of 51 slide scorers involved. The results of this StUdy show that even under these optimized conditions there is a great variation in the MN frequency or MNed cell frequency obtained by individual laboratories and scorers. All laboratories ranked correctly the MNed cell frequency in cells from cultures that were unirradiated, or exposed to 1 or 2 Gy of gamma rays. The study also estimated that the intra-scorer median coefficient of variation for duplicate MNed cell frequency scores is 29% for unexposed cultures and 14 and 11% for cells exposed to I and 2 Gy, respectively, These values can be used as a standard for quality or acceptability of data in future studies. Using a Poisson regression model it was estimated that radiation dose explained 67% of the variance, while staining method. cell sample, laboratory, and covariance explained 0.6, 0.3, 6.5. and 25.6% of the variance, respectively, leaving only 3.1% of the variance unexplained. As part of this exercise. nucleoplasmic bridges were also estimated by the laboratories however, inexperience in the use of this biomarker of chromosome rearrangement was reflected in the much greater heterogeneity in the data and the unexplained variation estimated by the Poisson model. The results of these studies indicate clearly that even after standardizing culture and scoring conditions it will be necessary to calibrate scorers and laboratories if MN, MNed cell and nucleoplasmic bridge frequencies are to be reliably compared among laboratories and among populations. (C) 2002 Elsevier Science B.V. All rights reserved.",
journal = "Mutation Research: Genetic Toxicology and Environmental Mutagenesis",
title = "Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes Results of an international slide-scoring exercise by the HUMN project",
volume = "534",
number = "1-2",
pages = "45-64",
doi = "10.1016/S1383-5718(02)00248-6"
}

Fenech, M., Bonassi, S., Turner, J., Lando, C., Ceppi, M., Chang, W., Holland, N., Kirsch-Volders, M., Zeiger, E., Bigatti, M., Bolognesi, C., Cao, J., De Luca, G., Di Giorgio, M., Ferguson, L., Fucic, A., Lima, O., Hadjidekova, V., Hrelia, P., Jaworska, A., Joksić, G., Krishnaja, A., Lee, T., Martelli, A., McKay, M., Migliore, L., Mirkova, E., Muller, W., Odagiri, Y., Orsiere, T., Scarfi, M., Silva, M., Sofuni, T., Suralles, J., Trenta, G., Vorobtsova, I., Vral, A.,& Zijno, A.. (2003). Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes Results of an international slide-scoring exercise by the HUMN project. in Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 534(1-2), 45-64.
https://doi.org/10.1016/S1383-5718(02)00248-6

Fenech M, Bonassi S, Turner J, Lando C, Ceppi M, Chang W, Holland N, Kirsch-Volders M, Zeiger E, Bigatti M, Bolognesi C, Cao J, De Luca G, Di Giorgio M, Ferguson L, Fucic A, Lima O, Hadjidekova V, Hrelia P, Jaworska A, Joksić G, Krishnaja A, Lee T, Martelli A, McKay M, Migliore L, Mirkova E, Muller W, Odagiri Y, Orsiere T, Scarfi M, Silva M, Sofuni T, Suralles J, Trenta G, Vorobtsova I, Vral A, Zijno A. Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes Results of an international slide-scoring exercise by the HUMN project. in Mutation Research: Genetic Toxicology and Environmental Mutagenesis. 2003;534(1-2):45-64.
doi:10.1016/S1383-5718(02)00248-6 .

Fenech, M, Bonassi, S, Turner, J, Lando, C, Ceppi, M, Chang, WP, Holland, N, Kirsch-Volders, M, Zeiger, E, Bigatti, MP, Bolognesi, C, Cao, J, De Luca, G, Di Giorgio, M, Ferguson, LR, Fucic, A, Lima, OG, Hadjidekova, VV, Hrelia, P, Jaworska, A, Joksić, Gordana, Krishnaja, AP, Lee, TK, Martelli, A, McKay, MJ, Migliore, L, Mirkova, E, Muller, WU, Odagiri, Y, Orsiere, T, Scarfi, MR, Silva, MJ, Sofuni, T, Suralles, J, Trenta, G, Vorobtsova, I, Vral, A, Zijno, A, "Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes Results of an international slide-scoring exercise by the HUMN project" in Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 534, no. 1-2 (2003):45-64,
https://doi.org/10.1016/S1383-5718(02)00248-6 . .