TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Albornoz, Karin
AU  - Belchi, Maria Dolores Lopez
AU  - Giordano, Ady
AU  - Bravo-Arrepol, Gaston
AU  - Moya-Elizondo, Ernesto
AU  - Martin, Juan San
AU  - Valdes, Oscar
AU  - Nešić, Aleksandra
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12870
AB  - In this work, chitosan films loaded with gallic acid and different content of chitin nanofibers were prepared and subjected to different characterization techniques. The results showed that the inclusion of gallic acid to chitosan films caused moderate decrease in water vapor permeability (by 29 %) and increased tensile strength of films (by 169 %) in comparison to the neat chitosan films. Furthermore, it was found that the addition of chitin nanofibers up to 30 % into chitosan/gallic acid films additionally improved tensile strength (by 474 %) and reduced plasticity of films (by 171 %), when compared to the chitosan/gallic acid films. Increased concentration of chitin nanofibers in films reduced the overall water vapor permeability of films by 51 %. In addition, gallic acid and chitin nanofibers had synergic effect on high chitosan film's antioxidant and antifungal activity toward Botrytis cinerea (both above 95 %). Finally, chitosan/gallic acid/chitin nanofibers films reduced decay incidence of strawberries, increased total soluble solid content, and promoted high production of some polyphenols during cold storage, in comparison to the control chitosan films and uncoated strawberry samples. Hence, these results suggest that chitosan/gallic acid/chitin nanofibers can present eco-sustainable approach for preservation of strawberries, giving them additional nutritional value.
T2  - International Journal of Biological Macromolecules
T1  - Influence of chitin nanofibers and gallic acid on physical-chemical and biological performances of chitosan-based films
VL  - 263, Part 1
SP  - 130159
DO  - 10.1016/j.ijbiomac.2024.130159
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Albornoz, Karin and Belchi, Maria Dolores Lopez and Giordano, Ady and Bravo-Arrepol, Gaston and Moya-Elizondo, Ernesto and Martin, Juan San and Valdes, Oscar and Nešić, Aleksandra",
year = "2024",
abstract = "In this work, chitosan films loaded with gallic acid and different content of chitin nanofibers were prepared and subjected to different characterization techniques. The results showed that the inclusion of gallic acid to chitosan films caused moderate decrease in water vapor permeability (by 29 %) and increased tensile strength of films (by 169 %) in comparison to the neat chitosan films. Furthermore, it was found that the addition of chitin nanofibers up to 30 % into chitosan/gallic acid films additionally improved tensile strength (by 474 %) and reduced plasticity of films (by 171 %), when compared to the chitosan/gallic acid films. Increased concentration of chitin nanofibers in films reduced the overall water vapor permeability of films by 51 %. In addition, gallic acid and chitin nanofibers had synergic effect on high chitosan film's antioxidant and antifungal activity toward Botrytis cinerea (both above 95 %). Finally, chitosan/gallic acid/chitin nanofibers films reduced decay incidence of strawberries, increased total soluble solid content, and promoted high production of some polyphenols during cold storage, in comparison to the control chitosan films and uncoated strawberry samples. Hence, these results suggest that chitosan/gallic acid/chitin nanofibers can present eco-sustainable approach for preservation of strawberries, giving them additional nutritional value.",
journal = "International Journal of Biological Macromolecules",
title = "Influence of chitin nanofibers and gallic acid on physical-chemical and biological performances of chitosan-based films",
volume = "263, Part 1",
pages = "130159",
doi = "10.1016/j.ijbiomac.2024.130159"
}

Cabrera-Barjas, G., Albornoz, K., Belchi, M. D. L., Giordano, A., Bravo-Arrepol, G., Moya-Elizondo, E., Martin, J. S., Valdes, O.,& Nešić, A.. (2024). Influence of chitin nanofibers and gallic acid on physical-chemical and biological performances of chitosan-based films. in International Journal of Biological Macromolecules, 263, Part 1, 130159.
https://doi.org/10.1016/j.ijbiomac.2024.130159

Cabrera-Barjas G, Albornoz K, Belchi MDL, Giordano A, Bravo-Arrepol G, Moya-Elizondo E, Martin JS, Valdes O, Nešić A. Influence of chitin nanofibers and gallic acid on physical-chemical and biological performances of chitosan-based films. in International Journal of Biological Macromolecules. 2024;263, Part 1:130159.
doi:10.1016/j.ijbiomac.2024.130159 .

Cabrera-Barjas, Gustavo, Albornoz, Karin, Belchi, Maria Dolores Lopez, Giordano, Ady, Bravo-Arrepol, Gaston, Moya-Elizondo, Ernesto, Martin, Juan San, Valdes, Oscar, Nešić, Aleksandra, "Influence of chitin nanofibers and gallic acid on physical-chemical and biological performances of chitosan-based films" in International Journal of Biological Macromolecules, 263, Part 1 (2024):130159,
https://doi.org/10.1016/j.ijbiomac.2024.130159 . .

TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Jimenez, Romel
AU  - Romero, Romina
AU  - Valdes, Oscar
AU  - Nešić, Aleksandra
AU  - Hernández-García, Ruber
AU  - Neira, Andrónico
AU  - Alejandro-Martín, Serguei
AU  - de la Torre, Alexander F.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10773
AB  - In this work, chitin, as a biobased polymer, is used as a precursor to obtain a phosphorylated derivatives. The influence of the different degree of phosphorylation in chitin on pyrolysis pattern was investigated. In order to understand the pyrolysis mechanism and the potential application of phosphorylated chitins, the samples were pyrolyzed at different temperatures and analyzed by FTIR, SEM, and Py-GC/MS analysis. Moreover, the thermal degradation and the evolved gases during chitin degradation and its derivatives were measured. The results showed that phosphorylation of chitin decreased the thermal stability of biopolymer and significantly changed the pattern of pyrolysis compared to neat chitin. The production of long-chain hydrocarbons was detected during pyrolysis of phosphorylated chitin, whereas this was not the case with raw chitin. Those two effects were more pronounced as the degree of phosphorylation increased. Chitin with the degree of phosphorylation (DS 1.35) exhibited the highest selectivity (91 %) towards production of long-chain hydrocarbons (C12-C17) at 500 °C. Moreover, the obtained results allowed to propose, for the first time, the mechanism of pyrolysis of phosphorylated chitin.
T2  - International Journal of Biological Macromolecules
T1  - Value-added long-chain aliphatic compounds obtained through pyrolysis of phosphorylated chitin
VL  - 238
SP  - 124130
DO  - 10.1016/j.ijbiomac.2023.124130
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Jimenez, Romel and Romero, Romina and Valdes, Oscar and Nešić, Aleksandra and Hernández-García, Ruber and Neira, Andrónico and Alejandro-Martín, Serguei and de la Torre, Alexander F.",
year = "2023",
abstract = "In this work, chitin, as a biobased polymer, is used as a precursor to obtain a phosphorylated derivatives. The influence of the different degree of phosphorylation in chitin on pyrolysis pattern was investigated. In order to understand the pyrolysis mechanism and the potential application of phosphorylated chitins, the samples were pyrolyzed at different temperatures and analyzed by FTIR, SEM, and Py-GC/MS analysis. Moreover, the thermal degradation and the evolved gases during chitin degradation and its derivatives were measured. The results showed that phosphorylation of chitin decreased the thermal stability of biopolymer and significantly changed the pattern of pyrolysis compared to neat chitin. The production of long-chain hydrocarbons was detected during pyrolysis of phosphorylated chitin, whereas this was not the case with raw chitin. Those two effects were more pronounced as the degree of phosphorylation increased. Chitin with the degree of phosphorylation (DS 1.35) exhibited the highest selectivity (91 %) towards production of long-chain hydrocarbons (C12-C17) at 500 °C. Moreover, the obtained results allowed to propose, for the first time, the mechanism of pyrolysis of phosphorylated chitin.",
journal = "International Journal of Biological Macromolecules",
title = "Value-added long-chain aliphatic compounds obtained through pyrolysis of phosphorylated chitin",
volume = "238",
pages = "124130",
doi = "10.1016/j.ijbiomac.2023.124130"
}

Cabrera-Barjas, G., Jimenez, R., Romero, R., Valdes, O., Nešić, A., Hernández-García, R., Neira, A., Alejandro-Martín, S.,& de la Torre, A. F.. (2023). Value-added long-chain aliphatic compounds obtained through pyrolysis of phosphorylated chitin. in International Journal of Biological Macromolecules, 238, 124130.
https://doi.org/10.1016/j.ijbiomac.2023.124130

Cabrera-Barjas G, Jimenez R, Romero R, Valdes O, Nešić A, Hernández-García R, Neira A, Alejandro-Martín S, de la Torre AF. Value-added long-chain aliphatic compounds obtained through pyrolysis of phosphorylated chitin. in International Journal of Biological Macromolecules. 2023;238:124130.
doi:10.1016/j.ijbiomac.2023.124130 .

Cabrera-Barjas, Gustavo, Jimenez, Romel, Romero, Romina, Valdes, Oscar, Nešić, Aleksandra, Hernández-García, Ruber, Neira, Andrónico, Alejandro-Martín, Serguei, de la Torre, Alexander F., "Value-added long-chain aliphatic compounds obtained through pyrolysis of phosphorylated chitin" in International Journal of Biological Macromolecules, 238 (2023):124130,
https://doi.org/10.1016/j.ijbiomac.2023.124130 . .

TY  - JOUR
AU  - Giordano, Ady
AU  - Morales-Tapia, Pablo
AU  - Moncada-Basualto, Mauricio
AU  - Pozo-Martínez, Josué
AU  - Olea-Azar, Claudio
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10130
AB  - Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.
T2  - Molecules
T1  - Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots
VL  - 27
IS  - 3
SP  - 610
DO  - 10.3390/molecules27030610
ER  - 

@article{
author = "Giordano, Ady and Morales-Tapia, Pablo and Moncada-Basualto, Mauricio and Pozo-Martínez, Josué and Olea-Azar, Claudio and Nešić, Aleksandra and Cabrera-Barjas, Gustavo",
year = "2022",
abstract = "Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.",
journal = "Molecules",
title = "Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots",
volume = "27",
number = "3",
pages = "610",
doi = "10.3390/molecules27030610"
}

Giordano, A., Morales-Tapia, P., Moncada-Basualto, M., Pozo-Martínez, J., Olea-Azar, C., Nešić, A.,& Cabrera-Barjas, G.. (2022). Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots. in Molecules, 27(3), 610.
https://doi.org/10.3390/molecules27030610

Giordano A, Morales-Tapia P, Moncada-Basualto M, Pozo-Martínez J, Olea-Azar C, Nešić A, Cabrera-Barjas G. Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots. in Molecules. 2022;27(3):610.
doi:10.3390/molecules27030610 .

Giordano, Ady, Morales-Tapia, Pablo, Moncada-Basualto, Mauricio, Pozo-Martínez, Josué, Olea-Azar, Claudio, Nešić, Aleksandra, Cabrera-Barjas, Gustavo, "Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots" in Molecules, 27, no. 3 (2022):610,
https://doi.org/10.3390/molecules27030610 . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Meseldžija, Slađana
AU  - Cabrera-Barjas, Gustavo
AU  - Onjia, Antonije E.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10149
AB  - Pectin is a natural biopolymer with broad applications in the food industry and it is suitable to prepare edible films to prolong food shelf-life. However, the main limitation of pectin-based films is their poor mechanical and barrier properties. Zeolite Y is a hydrophobic clay that can be used as film reinforcement material to improve its physicochemical and mechanical properties. In this work, the influence of high methoxyl citrus and apple pectin on physicochemical properties of biopolymer films modified with zeolite Y (0.05–0.2 wt%) was investigated. The films were characterized by FTIR, TGA, WAXD, mechanical analysis, and water vapor permeability analysis, and a potential film application is presented. The WAXD and FTIR analysis demonstrated that the strongest interaction between pectin chains and zeolite Y occurred when citrus high methylated pectin was used. Adding 0.2 wt% of zeolite Y into citrus high methylated pectin matrix enhanced the tensile strength by 66%, thermal stability by 13%, and water vapor barrier by 54%. In addition, fruit shelf-life test was performed, where strawberries were sealed in film. It was shown that sealed strawberries maintained a better color and healthy appearance than the control treatment after 7 days at 10◦ C. This study enabled the development of biocomposite films with improved properties for potential application in food packaging. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
T2  - Foods
T1  - Novel Biocomposite Films Based on High Methoxyl Pectin Reinforced with Zeolite Y for Food Packaging Applications
VL  - 11
IS  - 3
SP  - 360
DO  - 10.3390/foods11030360
ER  - 

@article{
author = "Nešić, Aleksandra and Meseldžija, Slađana and Cabrera-Barjas, Gustavo and Onjia, Antonije E.",
year = "2022",
abstract = "Pectin is a natural biopolymer with broad applications in the food industry and it is suitable to prepare edible films to prolong food shelf-life. However, the main limitation of pectin-based films is their poor mechanical and barrier properties. Zeolite Y is a hydrophobic clay that can be used as film reinforcement material to improve its physicochemical and mechanical properties. In this work, the influence of high methoxyl citrus and apple pectin on physicochemical properties of biopolymer films modified with zeolite Y (0.05–0.2 wt%) was investigated. The films were characterized by FTIR, TGA, WAXD, mechanical analysis, and water vapor permeability analysis, and a potential film application is presented. The WAXD and FTIR analysis demonstrated that the strongest interaction between pectin chains and zeolite Y occurred when citrus high methylated pectin was used. Adding 0.2 wt% of zeolite Y into citrus high methylated pectin matrix enhanced the tensile strength by 66%, thermal stability by 13%, and water vapor barrier by 54%. In addition, fruit shelf-life test was performed, where strawberries were sealed in film. It was shown that sealed strawberries maintained a better color and healthy appearance than the control treatment after 7 days at 10◦ C. This study enabled the development of biocomposite films with improved properties for potential application in food packaging. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
journal = "Foods",
title = "Novel Biocomposite Films Based on High Methoxyl Pectin Reinforced with Zeolite Y for Food Packaging Applications",
volume = "11",
number = "3",
pages = "360",
doi = "10.3390/foods11030360"
}

Nešić, A., Meseldžija, S., Cabrera-Barjas, G.,& Onjia, A. E.. (2022). Novel Biocomposite Films Based on High Methoxyl Pectin Reinforced with Zeolite Y for Food Packaging Applications. in Foods, 11(3), 360.
https://doi.org/10.3390/foods11030360

Nešić A, Meseldžija S, Cabrera-Barjas G, Onjia AE. Novel Biocomposite Films Based on High Methoxyl Pectin Reinforced with Zeolite Y for Food Packaging Applications. in Foods. 2022;11(3):360.
doi:10.3390/foods11030360 .

Nešić, Aleksandra, Meseldžija, Slađana, Cabrera-Barjas, Gustavo, Onjia, Antonije E., "Novel Biocomposite Films Based on High Methoxyl Pectin Reinforced with Zeolite Y for Food Packaging Applications" in Foods, 11, no. 3 (2022):360,
https://doi.org/10.3390/foods11030360 . .

TY  - JOUR
AU  - Ponce, Giovanni
AU  - Rodríguez-Llamazares, Saddys
AU  - Rivera, Patricia Castaño
AU  - Castaño, Johanna
AU  - Oporto-Velásquez, Gloria
AU  - Sabando, Constanza
AU  - Ide, Walter
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10378
AB  - Quinoa husk (QH) is often discarded without being utilized. This study investigates an alternative route for this agro-waste resource as a raw material in the preparation of bioplastic composites. The effect of QH on the mechanical and thermal properties of thermoplastic poly(lactic acid)/poly(butylene adipate-co-terephthalate) (PLA/PBAT) blends was evaluated. The composites were prepared by melting compounding. The content of quinoa husk in composite materials varied from 0-30 wt%. The results demonstrated that addition of quinoa husk in PLA/PBAT blend induced slight increase in crystalline phase of PLA, which was evidenced by FTIR and DSC analysis. However, it reduced the mechanical and thermal properties of materials. The 30 wt% load of quinoa husk in formulation led to decreased tensile strength (by 45.5%), elongation at break (by 85.8%), thermal stability (by 23.5%) and flexural strength (by 36%) of final biocomposite material, when compared to the control biopolymer blend. Although addition of quinoa husk in bioplastic material did not improve mechanical properties, load of 20 wt% QH in PLA/PBAT still provided satisfied tensile strength of 19 MPa and flexural strength of 27 MPa, showing that quinoa husk can add value to lignocellulosic by-product/agro waste, particularly decreasing the cost and increasing the sustainability of bioplastic materials for use in the agriculture industry.
T2  - Journal of Polymer Research
T1  - Biocomoposites of polylactic acid/ poly(butylene adipate-co-terephthalate) blends loaded with quinoa husk agro-waste: thermal and mechanical properties
VL  - 29
IS  - 8
SP  - 356
DO  - 10.1007/s10965-022-03196-y
ER  - 

@article{
author = "Ponce, Giovanni and Rodríguez-Llamazares, Saddys and Rivera, Patricia Castaño and Castaño, Johanna and Oporto-Velásquez, Gloria and Sabando, Constanza and Ide, Walter and Nešić, Aleksandra and Cabrera-Barjas, Gustavo",
year = "2022",
abstract = "Quinoa husk (QH) is often discarded without being utilized. This study investigates an alternative route for this agro-waste resource as a raw material in the preparation of bioplastic composites. The effect of QH on the mechanical and thermal properties of thermoplastic poly(lactic acid)/poly(butylene adipate-co-terephthalate) (PLA/PBAT) blends was evaluated. The composites were prepared by melting compounding. The content of quinoa husk in composite materials varied from 0-30 wt%. The results demonstrated that addition of quinoa husk in PLA/PBAT blend induced slight increase in crystalline phase of PLA, which was evidenced by FTIR and DSC analysis. However, it reduced the mechanical and thermal properties of materials. The 30 wt% load of quinoa husk in formulation led to decreased tensile strength (by 45.5%), elongation at break (by 85.8%), thermal stability (by 23.5%) and flexural strength (by 36%) of final biocomposite material, when compared to the control biopolymer blend. Although addition of quinoa husk in bioplastic material did not improve mechanical properties, load of 20 wt% QH in PLA/PBAT still provided satisfied tensile strength of 19 MPa and flexural strength of 27 MPa, showing that quinoa husk can add value to lignocellulosic by-product/agro waste, particularly decreasing the cost and increasing the sustainability of bioplastic materials for use in the agriculture industry.",
journal = "Journal of Polymer Research",
title = "Biocomoposites of polylactic acid/ poly(butylene adipate-co-terephthalate) blends loaded with quinoa husk agro-waste: thermal and mechanical properties",
volume = "29",
number = "8",
pages = "356",
doi = "10.1007/s10965-022-03196-y"
}

Ponce, G., Rodríguez-Llamazares, S., Rivera, P. C., Castaño, J., Oporto-Velásquez, G., Sabando, C., Ide, W., Nešić, A.,& Cabrera-Barjas, G.. (2022). Biocomoposites of polylactic acid/ poly(butylene adipate-co-terephthalate) blends loaded with quinoa husk agro-waste: thermal and mechanical properties. in Journal of Polymer Research, 29(8), 356.
https://doi.org/10.1007/s10965-022-03196-y

Ponce G, Rodríguez-Llamazares S, Rivera PC, Castaño J, Oporto-Velásquez G, Sabando C, Ide W, Nešić A, Cabrera-Barjas G. Biocomoposites of polylactic acid/ poly(butylene adipate-co-terephthalate) blends loaded with quinoa husk agro-waste: thermal and mechanical properties. in Journal of Polymer Research. 2022;29(8):356.
doi:10.1007/s10965-022-03196-y .

Ponce, Giovanni, Rodríguez-Llamazares, Saddys, Rivera, Patricia Castaño, Castaño, Johanna, Oporto-Velásquez, Gloria, Sabando, Constanza, Ide, Walter, Nešić, Aleksandra, Cabrera-Barjas, Gustavo, "Biocomoposites of polylactic acid/ poly(butylene adipate-co-terephthalate) blends loaded with quinoa husk agro-waste: thermal and mechanical properties" in Journal of Polymer Research, 29, no. 8 (2022):356,
https://doi.org/10.1007/s10965-022-03196-y . .

TY  - JOUR
AU  - Figueroa, Fabian A.
AU  - Abdala-Díaz, Roberto T.
AU  - Pérez, Claudia
AU  - Casas-Arrojo, Virginia
AU  - Nešić, Aleksandra
AU  - Tapia, Cecilia
AU  - Durán, Carla
AU  - Valdes, Oscar
AU  - Parra, Carolina
AU  - Bravo-Arrepol, Gastón
AU  - Soto, Luis
AU  - Becerra, José
AU  - Cabrera-Barjas, Gustavo
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10388
AB  - Codium bernabei is a green alga that grows on Chilean coasts. The composition of its structural polysaccharides is still unknown. Hence, the aim of this work is to isolate and characterize the hot water extracted polysaccharide fractions. For this purpose, the water extracts were further precipitated in alcohol (TPs) and acid media (APs), respectively. Both fractions were characterized using different physicochemical techniques such as GC-MS, GPC, FTIR, TGA, and SEM. It is confirmed that the extracted fractions are mainly made of sulfated galactan unit, with a degree of sulfation of 19.3% (TPs) and 17.4% (ATs) and a protein content of 3.5% in APs and 15.6% in TPs. Other neutral sugars such as xylose, glucose, galactose, fucose, mannose, and arabinose were found in a molar ratio (0.05:0.6:1.0:0.02:0.14:0.11) for TPs and (0.05:0.31:1.0:0.03:0.1:0.13) for ATs. The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset > 190 °C) and showed antioxidant activity according to the ABTS•+ and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. The PT and APTTS assays were used to measure the anticoagulant activity of the polysaccharide fractions. In general, the PT activity of the TPs and APs was not different from normal plasma values. The exception was the TPs treatment at 1000 µg mL−1 concentration. The APTTS test revealed that clotting time for both polysaccharides was prolonged regarding normal values at 1000 µg mL−1. Finally, the antitumor test in colorectal carcinoma (HTC-116) cell line, breast cancer (MCF-7) and human leukemia (HL-60) cell lines showed the cytotoxic effect of TPs and APs. Those results suggest the potential biotechnological application of sulfate galactan polysaccharides isolated from a Chilean marine resource.
T2  - Marine Drugs
T1  - Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity
VL  - 20
IS  - 7
SP  - 458
DO  - 10.3390/md20070458
ER  - 

@article{
author = "Figueroa, Fabian A. and Abdala-Díaz, Roberto T. and Pérez, Claudia and Casas-Arrojo, Virginia and Nešić, Aleksandra and Tapia, Cecilia and Durán, Carla and Valdes, Oscar and Parra, Carolina and Bravo-Arrepol, Gastón and Soto, Luis and Becerra, José and Cabrera-Barjas, Gustavo",
year = "2022",
abstract = "Codium bernabei is a green alga that grows on Chilean coasts. The composition of its structural polysaccharides is still unknown. Hence, the aim of this work is to isolate and characterize the hot water extracted polysaccharide fractions. For this purpose, the water extracts were further precipitated in alcohol (TPs) and acid media (APs), respectively. Both fractions were characterized using different physicochemical techniques such as GC-MS, GPC, FTIR, TGA, and SEM. It is confirmed that the extracted fractions are mainly made of sulfated galactan unit, with a degree of sulfation of 19.3% (TPs) and 17.4% (ATs) and a protein content of 3.5% in APs and 15.6% in TPs. Other neutral sugars such as xylose, glucose, galactose, fucose, mannose, and arabinose were found in a molar ratio (0.05:0.6:1.0:0.02:0.14:0.11) for TPs and (0.05:0.31:1.0:0.03:0.1:0.13) for ATs. The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset > 190 °C) and showed antioxidant activity according to the ABTS•+ and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. The PT and APTTS assays were used to measure the anticoagulant activity of the polysaccharide fractions. In general, the PT activity of the TPs and APs was not different from normal plasma values. The exception was the TPs treatment at 1000 µg mL−1 concentration. The APTTS test revealed that clotting time for both polysaccharides was prolonged regarding normal values at 1000 µg mL−1. Finally, the antitumor test in colorectal carcinoma (HTC-116) cell line, breast cancer (MCF-7) and human leukemia (HL-60) cell lines showed the cytotoxic effect of TPs and APs. Those results suggest the potential biotechnological application of sulfate galactan polysaccharides isolated from a Chilean marine resource.",
journal = "Marine Drugs",
title = "Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity",
volume = "20",
number = "7",
pages = "458",
doi = "10.3390/md20070458"
}

Figueroa, F. A., Abdala-Díaz, R. T., Pérez, C., Casas-Arrojo, V., Nešić, A., Tapia, C., Durán, C., Valdes, O., Parra, C., Bravo-Arrepol, G., Soto, L., Becerra, J.,& Cabrera-Barjas, G.. (2022). Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity. in Marine Drugs, 20(7), 458.
https://doi.org/10.3390/md20070458

Figueroa FA, Abdala-Díaz RT, Pérez C, Casas-Arrojo V, Nešić A, Tapia C, Durán C, Valdes O, Parra C, Bravo-Arrepol G, Soto L, Becerra J, Cabrera-Barjas G. Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity. in Marine Drugs. 2022;20(7):458.
doi:10.3390/md20070458 .

Figueroa, Fabian A., Abdala-Díaz, Roberto T., Pérez, Claudia, Casas-Arrojo, Virginia, Nešić, Aleksandra, Tapia, Cecilia, Durán, Carla, Valdes, Oscar, Parra, Carolina, Bravo-Arrepol, Gastón, Soto, Luis, Becerra, José, Cabrera-Barjas, Gustavo, "Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity" in Marine Drugs, 20, no. 7 (2022):458,
https://doi.org/10.3390/md20070458 . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Meseldžija, Slađana
AU  - Onjia, Antonije E.
AU  - Cabrera-Barjas, Gustavo
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10543
AB  - In this research, the pectin monoliths were prepared via the sol-gel process through different routes of crosslinking and additional freeze-drying. The crosslinking reaction was induced by the use of calcium ions in aqueous solutions and in alcohol/water solutions. The resulting pectin monoliths obtained by freeze-drying were macroporous with open cells, limited specific surface area, moderate mechanical stability and moderate biodegradation rate. The presence of alcohol in crosslinking solution significantly changed the morphology of final pectin monoliths, which was evidenced by the reduction of their pore size for one order. The specific surface area of pectin monoliths obtained through the calcium-water-alcohol route was 25.7 m2/g, the Young compressive modulus was 0.52 MPa, and the biodegradation rate was 45% after 30 days of immersion in compost media. Considering that pectin can be obtained from food waste, and its physical properties could be tailored by different crosslinking routes, the pectin monoliths could find wide application in the pharmaceutical, agricultural, medical and food industries, providing sustainable development concepts.
T2  - Polymers
T1  - Impact of Crosslinking on the Characteristics of Pectin Monolith Cryogels
VL  - 14
IS  - 23
SP  - 5252
DO  - 10.3390/polym14235252
ER  - 

@article{
author = "Nešić, Aleksandra and Meseldžija, Slađana and Onjia, Antonije E. and Cabrera-Barjas, Gustavo",
year = "2022",
abstract = "In this research, the pectin monoliths were prepared via the sol-gel process through different routes of crosslinking and additional freeze-drying. The crosslinking reaction was induced by the use of calcium ions in aqueous solutions and in alcohol/water solutions. The resulting pectin monoliths obtained by freeze-drying were macroporous with open cells, limited specific surface area, moderate mechanical stability and moderate biodegradation rate. The presence of alcohol in crosslinking solution significantly changed the morphology of final pectin monoliths, which was evidenced by the reduction of their pore size for one order. The specific surface area of pectin monoliths obtained through the calcium-water-alcohol route was 25.7 m2/g, the Young compressive modulus was 0.52 MPa, and the biodegradation rate was 45% after 30 days of immersion in compost media. Considering that pectin can be obtained from food waste, and its physical properties could be tailored by different crosslinking routes, the pectin monoliths could find wide application in the pharmaceutical, agricultural, medical and food industries, providing sustainable development concepts.",
journal = "Polymers",
title = "Impact of Crosslinking on the Characteristics of Pectin Monolith Cryogels",
volume = "14",
number = "23",
pages = "5252",
doi = "10.3390/polym14235252"
}

Nešić, A., Meseldžija, S., Onjia, A. E.,& Cabrera-Barjas, G.. (2022). Impact of Crosslinking on the Characteristics of Pectin Monolith Cryogels. in Polymers, 14(23), 5252.
https://doi.org/10.3390/polym14235252

Nešić A, Meseldžija S, Onjia AE, Cabrera-Barjas G. Impact of Crosslinking on the Characteristics of Pectin Monolith Cryogels. in Polymers. 2022;14(23):5252.
doi:10.3390/polym14235252 .

Nešić, Aleksandra, Meseldžija, Slađana, Onjia, Antonije E., Cabrera-Barjas, Gustavo, "Impact of Crosslinking on the Characteristics of Pectin Monolith Cryogels" in Polymers, 14, no. 23 (2022):5252,
https://doi.org/10.3390/polym14235252 . .

TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - González, Cristian
AU  - Nešić, Aleksandra
AU  - Marrugo, Kelly P
AU  - Gómez, Oscar
AU  - Delattre, Cédric
AU  - Valdes, Oscar
AU  - Yin, Heng
AU  - Bravo, Gaston
AU  - Cea, Juan
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9628
AB  - β-chitin was isolated from marine waste, giant Humboldt squid Dosidicus gigas, and further converted to nanofibers by use of a collider machine under acidic conditions (pH 3). The FTIR, TGA, and NMR analysis confirmed the efficient extraction of β-chitin. The SEM, TEM, and XRD characterization results verified that β-chitin crystalline structure were maintained after mechanical treatment. The mean particle size of β-chitin nanofibers was in the range between 10 and 15 nm, according to the TEM analysis. In addition, the β-chitin nanofibers were converted into films by the simple solvent-casting and drying process at 60 °C. The obtained films had high lightness, which was evidenced by the CIELAB color test. Moreover, the films showed the medium swelling degree (250–290%) in aqueous solutions of different pH and good mechanical resistance in the range between 4 and 17 MPa, depending on film thickness. The results obtained in this work show that marine waste can be efficiently converted to biomaterial by use of mild extractive conditions and simple mechanical treatment, offering great potential for the future development of sustainable multifunctional materials for various industrial applications such as food packaging, agriculture, and/or wound dressing.
T2  - Marine Drugs
T1  - Utilization of Marine Waste to Obtain β-Chitin Nanofibers and Films from Giant Humboldt Squid Dosidicus gigas
VL  - 19
IS  - 4
SP  - 184
DO  - 10.3390/md19040184
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and González, Cristian and Nešić, Aleksandra and Marrugo, Kelly P and Gómez, Oscar and Delattre, Cédric and Valdes, Oscar and Yin, Heng and Bravo, Gaston and Cea, Juan",
year = "2021",
abstract = "β-chitin was isolated from marine waste, giant Humboldt squid Dosidicus gigas, and further converted to nanofibers by use of a collider machine under acidic conditions (pH 3). The FTIR, TGA, and NMR analysis confirmed the efficient extraction of β-chitin. The SEM, TEM, and XRD characterization results verified that β-chitin crystalline structure were maintained after mechanical treatment. The mean particle size of β-chitin nanofibers was in the range between 10 and 15 nm, according to the TEM analysis. In addition, the β-chitin nanofibers were converted into films by the simple solvent-casting and drying process at 60 °C. The obtained films had high lightness, which was evidenced by the CIELAB color test. Moreover, the films showed the medium swelling degree (250–290%) in aqueous solutions of different pH and good mechanical resistance in the range between 4 and 17 MPa, depending on film thickness. The results obtained in this work show that marine waste can be efficiently converted to biomaterial by use of mild extractive conditions and simple mechanical treatment, offering great potential for the future development of sustainable multifunctional materials for various industrial applications such as food packaging, agriculture, and/or wound dressing.",
journal = "Marine Drugs",
title = "Utilization of Marine Waste to Obtain β-Chitin Nanofibers and Films from Giant Humboldt Squid Dosidicus gigas",
volume = "19",
number = "4",
pages = "184",
doi = "10.3390/md19040184"
}

Cabrera-Barjas, G., González, C., Nešić, A., Marrugo, K. P., Gómez, O., Delattre, C., Valdes, O., Yin, H., Bravo, G.,& Cea, J.. (2021). Utilization of Marine Waste to Obtain β-Chitin Nanofibers and Films from Giant Humboldt Squid Dosidicus gigas. in Marine Drugs, 19(4), 184.
https://doi.org/10.3390/md19040184

Cabrera-Barjas G, González C, Nešić A, Marrugo KP, Gómez O, Delattre C, Valdes O, Yin H, Bravo G, Cea J. Utilization of Marine Waste to Obtain β-Chitin Nanofibers and Films from Giant Humboldt Squid Dosidicus gigas. in Marine Drugs. 2021;19(4):184.
doi:10.3390/md19040184 .

Cabrera-Barjas, Gustavo, González, Cristian, Nešić, Aleksandra, Marrugo, Kelly P, Gómez, Oscar, Delattre, Cédric, Valdes, Oscar, Yin, Heng, Bravo, Gaston, Cea, Juan, "Utilization of Marine Waste to Obtain β-Chitin Nanofibers and Films from Giant Humboldt Squid Dosidicus gigas" in Marine Drugs, 19, no. 4 (2021):184,
https://doi.org/10.3390/md19040184 . .

TY  - JOUR
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Gordić, Milan V.
AU  - Cabrera-Barjas, Gustavo
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10068
AB  - The development of edible films and coatings in the food packaging industry presents one of the modern strategies for protecting food products and ensuring their freshness and quality during their shelf lives. The application of microbial polysaccharides to the development of food package materials, as an alternative option to the commonly used plastic materials, is both economic and environmentally favorable. New edible films were developed using dextran from lactic acid bacterium Leuconostoc mesenteroides T3, and additionally plasticized by different concentrations of polyglycerol. The best tensile strength of the films was obtained using a formulation that contained 10 wt% of polyglycerol, which corresponded to a value of 4.6 MPa. The most flexible formulation, with elongation at break of 602%, was obtained with 30 wt% of polyglycerol. Water vapor permeability values of the films synthesized in this study were in the range of (3.45–8.81) ∗ 10−12 g/m s Pa. Such low values indicated that they could be efficient in preventing fruit from drying out during storage. Thus, the film formulations were used to coat blueberries in order to assess their quality during a storage time of 21 days at 8 °C. The results showed that dextran/polyglycerol films could be efficient in extending the shelf life of blueberries, which was evidenced by lower weight loss and total sugar solids values, as well as a delay in titratable acidity, in comparison to the uncoated blueberries.
T2  - Polymers
T1  - Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries
VL  - 13
IS  - 23
SP  - 4252
DO  - 10.3390/polym13234252
ER  - 

@article{
author = "Davidović, Slađana and Miljković, Miona and Gordić, Milan V. and Cabrera-Barjas, Gustavo and Nešić, Aleksandra and Dimitrijević-Branković, Suzana I.",
year = "2021",
abstract = "The development of edible films and coatings in the food packaging industry presents one of the modern strategies for protecting food products and ensuring their freshness and quality during their shelf lives. The application of microbial polysaccharides to the development of food package materials, as an alternative option to the commonly used plastic materials, is both economic and environmentally favorable. New edible films were developed using dextran from lactic acid bacterium Leuconostoc mesenteroides T3, and additionally plasticized by different concentrations of polyglycerol. The best tensile strength of the films was obtained using a formulation that contained 10 wt% of polyglycerol, which corresponded to a value of 4.6 MPa. The most flexible formulation, with elongation at break of 602%, was obtained with 30 wt% of polyglycerol. Water vapor permeability values of the films synthesized in this study were in the range of (3.45–8.81) ∗ 10−12 g/m s Pa. Such low values indicated that they could be efficient in preventing fruit from drying out during storage. Thus, the film formulations were used to coat blueberries in order to assess their quality during a storage time of 21 days at 8 °C. The results showed that dextran/polyglycerol films could be efficient in extending the shelf life of blueberries, which was evidenced by lower weight loss and total sugar solids values, as well as a delay in titratable acidity, in comparison to the uncoated blueberries.",
journal = "Polymers",
title = "Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries",
volume = "13",
number = "23",
pages = "4252",
doi = "10.3390/polym13234252"
}

Davidović, S., Miljković, M., Gordić, M. V., Cabrera-Barjas, G., Nešić, A.,& Dimitrijević-Branković, S. I.. (2021). Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries. in Polymers, 13(23), 4252.
https://doi.org/10.3390/polym13234252

Davidović S, Miljković M, Gordić MV, Cabrera-Barjas G, Nešić A, Dimitrijević-Branković SI. Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries. in Polymers. 2021;13(23):4252.
doi:10.3390/polym13234252 .

Davidović, Slađana, Miljković, Miona, Gordić, Milan V., Cabrera-Barjas, Gustavo, Nešić, Aleksandra, Dimitrijević-Branković, Suzana I., "Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries" in Polymers, 13, no. 23 (2021):4252,
https://doi.org/10.3390/polym13234252 . .

TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Nešić, Aleksandra
AU  - Bravo-Arrepol, Gaston
AU  - Rodríguez-Llamazares, Saddys
AU  - Valdés, Oscar
AU  - Banerjee, Aparna
AU  - Castaño, Johanna
AU  - Delattre, Cédric
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10100
AB  - This study investigated the biocomposite pectin films enriched with murta (Ugni molinae T.) seed polyphenolic extract and reinforced by chitin nanofiber. The structural, morphological, mechanical, barrier, colorimetric, and antioxidant activity of films were evaluated. The obtained data clearly demonstrated that the addition of murta seed extract and the high load of chitin nanofibers (50%) provided more cohesive and dense morphology of films and improved the mechanical resistance and water vapor barrier in comparison to the control pectin film. The antioxidant activity ranged between 71% and 86%, depending on the film formulation and concentration of chitin nanofibers. The presented results highlight the potential use of chitin nanofibers and murta seed extract in the pectin matrix to be applied in functional food coatings and packaging, as a sustainable solution.
T2  - Molecules
T1  - Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers
VL  - 26
IS  - 24
SP  - 7477
DO  - 10.3390/molecules26247477
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Nešić, Aleksandra and Bravo-Arrepol, Gaston and Rodríguez-Llamazares, Saddys and Valdés, Oscar and Banerjee, Aparna and Castaño, Johanna and Delattre, Cédric",
year = "2021",
abstract = "This study investigated the biocomposite pectin films enriched with murta (Ugni molinae T.) seed polyphenolic extract and reinforced by chitin nanofiber. The structural, morphological, mechanical, barrier, colorimetric, and antioxidant activity of films were evaluated. The obtained data clearly demonstrated that the addition of murta seed extract and the high load of chitin nanofibers (50%) provided more cohesive and dense morphology of films and improved the mechanical resistance and water vapor barrier in comparison to the control pectin film. The antioxidant activity ranged between 71% and 86%, depending on the film formulation and concentration of chitin nanofibers. The presented results highlight the potential use of chitin nanofibers and murta seed extract in the pectin matrix to be applied in functional food coatings and packaging, as a sustainable solution.",
journal = "Molecules",
title = "Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers",
volume = "26",
number = "24",
pages = "7477",
doi = "10.3390/molecules26247477"
}

Cabrera-Barjas, G., Nešić, A., Bravo-Arrepol, G., Rodríguez-Llamazares, S., Valdés, O., Banerjee, A., Castaño, J.,& Delattre, C.. (2021). Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers. in Molecules, 26(24), 7477.
https://doi.org/10.3390/molecules26247477

Cabrera-Barjas G, Nešić A, Bravo-Arrepol G, Rodríguez-Llamazares S, Valdés O, Banerjee A, Castaño J, Delattre C. Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers. in Molecules. 2021;26(24):7477.
doi:10.3390/molecules26247477 .

Cabrera-Barjas, Gustavo, Nešić, Aleksandra, Bravo-Arrepol, Gaston, Rodríguez-Llamazares, Saddys, Valdés, Oscar, Banerjee, Aparna, Castaño, Johanna, Delattre, Cédric, "Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers" in Molecules, 26, no. 24 (2021):7477,
https://doi.org/10.3390/molecules26247477 . .

TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Radovanović, Neda
AU  - Arrepol, Gaston Bravo
AU  - de la Torre, Alexander F.
AU  - Valdes, Oscar
AU  - Nešić, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10700
AB  - The efficient use of waste from food processing industry is one of the innovative approaches within sustainable development, because it can be transferred into added value products, which could improve economic, energetic and environmental sectors. In this context, the squid pen waste from seafood industry was used as raw material to obtain nanofibrous beta-chitin films. In order to extend functionality of obtained films, elderberry extract obtained from biomass was added at different concentrations. The tensile strength of chitin-elderberry extract films was improved by 52%, elongation at break by 153% and water vapor barrier by 65%. The obtained material showed distinct color change when subjected to acidic or basic solutions. It was proven by CIELab color analysis that all color changes could be easily perceived visually. In addition, the obtained nanofibrous film was successfully used to monitor the freshness of Hake fish. Namely, when the film was introduced in a package that contained fresh fish, its color was efficiently changed within the time during the storage at 4 degrees C. The obtained results demonstrated that food processing waste could be efficiently valorized, and could give sustainable food package design as a spoilage indicator of high protein food.
T2  - International Journal of Biological Macromolecules
T1  - Valorization of food waste to produce intelligent nanofibrous beta-chitin films
VL  - 186
SP  - 92
EP  - 99
DO  - 10.1016/j.ijbiomac.2021.07.045
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Radovanović, Neda and Arrepol, Gaston Bravo and de la Torre, Alexander F. and Valdes, Oscar and Nešić, Aleksandra",
year = "2021",
abstract = "The efficient use of waste from food processing industry is one of the innovative approaches within sustainable development, because it can be transferred into added value products, which could improve economic, energetic and environmental sectors. In this context, the squid pen waste from seafood industry was used as raw material to obtain nanofibrous beta-chitin films. In order to extend functionality of obtained films, elderberry extract obtained from biomass was added at different concentrations. The tensile strength of chitin-elderberry extract films was improved by 52%, elongation at break by 153% and water vapor barrier by 65%. The obtained material showed distinct color change when subjected to acidic or basic solutions. It was proven by CIELab color analysis that all color changes could be easily perceived visually. In addition, the obtained nanofibrous film was successfully used to monitor the freshness of Hake fish. Namely, when the film was introduced in a package that contained fresh fish, its color was efficiently changed within the time during the storage at 4 degrees C. The obtained results demonstrated that food processing waste could be efficiently valorized, and could give sustainable food package design as a spoilage indicator of high protein food.",
journal = "International Journal of Biological Macromolecules",
title = "Valorization of food waste to produce intelligent nanofibrous beta-chitin films",
volume = "186",
pages = "92-99",
doi = "10.1016/j.ijbiomac.2021.07.045"
}

Cabrera-Barjas, G., Radovanović, N., Arrepol, G. B., de la Torre, A. F., Valdes, O.,& Nešić, A.. (2021). Valorization of food waste to produce intelligent nanofibrous beta-chitin films. in International Journal of Biological Macromolecules, 186, 92-99.
https://doi.org/10.1016/j.ijbiomac.2021.07.045

Cabrera-Barjas G, Radovanović N, Arrepol GB, de la Torre AF, Valdes O, Nešić A. Valorization of food waste to produce intelligent nanofibrous beta-chitin films. in International Journal of Biological Macromolecules. 2021;186:92-99.
doi:10.1016/j.ijbiomac.2021.07.045 .

Cabrera-Barjas, Gustavo, Radovanović, Neda, Arrepol, Gaston Bravo, de la Torre, Alexander F., Valdes, Oscar, Nešić, Aleksandra, "Valorization of food waste to produce intelligent nanofibrous beta-chitin films" in International Journal of Biological Macromolecules, 186 (2021):92-99,
https://doi.org/10.1016/j.ijbiomac.2021.07.045 . .

TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Quezada, Adriana
AU  - Bernardo, Yamilé
AU  - Moncada, Mauricio
AU  - Zúñiga, Elisa
AU  - Wilkens, Marcela
AU  - Giordano, Ady
AU  - Nešić, Aleksandra
AU  - Delgado, Nacarid
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8845
AB  - Murta (Ugni molinae Turcz) is an endemic Chilean specie mostly used in medical, cosmetic, and food industries. However, during the industrial processing of murta fruits, the biomass containing the seeds is discarded as an industrial byproduct that does not find significant uses yet. This work is a first approach to valorize murta biomass through the identification and quantification of principal chemical constituents and exploring their antibacterial properties. The proximal analysis revealed that murta seeds exhibited significant content of raw fiber (64%), crude fat (14%), crude protein (12%), and low levels of ashes (1.5%) and minerals (0.04–0.23%). Dietary fiber was mainly composed of lignin, cellulose, pectin, and hemicellulose. Polyunsaturated fatty acids (89.0%), monounsaturated fatty acids (7.7%), and saturated fatty acids (3.3%) were the main constituents of seed oils. The arginine, asparagine, glutamic acid, and glycine were the primary protein constituent amino acids. Tannin fractions, total polyphenolic content, and oxygen radical absorbance capacity as antioxidant activity were measured. The chromatographic and mass spectrometric analysis (HPLC–MS/MS) confirmed the presence of several phenolic compounds like phenolic acids, flavonols, flavones, proanthocyanidins, and high molecular weight polyphenols. The murta seed extract showed high antibacterial activity against both Gram positive (Staphylococcus aureus, Bacillus cereus, and Streptococcus pyogenes) and Gram negative (Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa) bacterial strains. Murta seeds could be considered as a new source of nutritional components and bioactive compounds for different nutraceutical and food applications. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Journal of Food Measurement and Characterization
T1  - Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds: an undervalued Chilean resource
VL  - 14
IS  - 4
SP  - 1810
EP  - 1821
DO  - 10.1007/s11694-020-00428-x
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Quezada, Adriana and Bernardo, Yamilé and Moncada, Mauricio and Zúñiga, Elisa and Wilkens, Marcela and Giordano, Ady and Nešić, Aleksandra and Delgado, Nacarid",
year = "2020",
abstract = "Murta (Ugni molinae Turcz) is an endemic Chilean specie mostly used in medical, cosmetic, and food industries. However, during the industrial processing of murta fruits, the biomass containing the seeds is discarded as an industrial byproduct that does not find significant uses yet. This work is a first approach to valorize murta biomass through the identification and quantification of principal chemical constituents and exploring their antibacterial properties. The proximal analysis revealed that murta seeds exhibited significant content of raw fiber (64%), crude fat (14%), crude protein (12%), and low levels of ashes (1.5%) and minerals (0.04–0.23%). Dietary fiber was mainly composed of lignin, cellulose, pectin, and hemicellulose. Polyunsaturated fatty acids (89.0%), monounsaturated fatty acids (7.7%), and saturated fatty acids (3.3%) were the main constituents of seed oils. The arginine, asparagine, glutamic acid, and glycine were the primary protein constituent amino acids. Tannin fractions, total polyphenolic content, and oxygen radical absorbance capacity as antioxidant activity were measured. The chromatographic and mass spectrometric analysis (HPLC–MS/MS) confirmed the presence of several phenolic compounds like phenolic acids, flavonols, flavones, proanthocyanidins, and high molecular weight polyphenols. The murta seed extract showed high antibacterial activity against both Gram positive (Staphylococcus aureus, Bacillus cereus, and Streptococcus pyogenes) and Gram negative (Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa) bacterial strains. Murta seeds could be considered as a new source of nutritional components and bioactive compounds for different nutraceutical and food applications. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Journal of Food Measurement and Characterization",
title = "Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds: an undervalued Chilean resource",
volume = "14",
number = "4",
pages = "1810-1821",
doi = "10.1007/s11694-020-00428-x"
}

Cabrera-Barjas, G., Quezada, A., Bernardo, Y., Moncada, M., Zúñiga, E., Wilkens, M., Giordano, A., Nešić, A.,& Delgado, N.. (2020). Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds: an undervalued Chilean resource. in Journal of Food Measurement and Characterization, 14(4), 1810-1821.
https://doi.org/10.1007/s11694-020-00428-x

Cabrera-Barjas G, Quezada A, Bernardo Y, Moncada M, Zúñiga E, Wilkens M, Giordano A, Nešić A, Delgado N. Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds: an undervalued Chilean resource. in Journal of Food Measurement and Characterization. 2020;14(4):1810-1821.
doi:10.1007/s11694-020-00428-x .

Cabrera-Barjas, Gustavo, Quezada, Adriana, Bernardo, Yamilé, Moncada, Mauricio, Zúñiga, Elisa, Wilkens, Marcela, Giordano, Ady, Nešić, Aleksandra, Delgado, Nacarid, "Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds: an undervalued Chilean resource" in Journal of Food Measurement and Characterization, 14, no. 4 (2020):1810-1821,
https://doi.org/10.1007/s11694-020-00428-x . .

TY  - JOUR
AU  - Díaz-Galindo, Edaena Pamela
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Mardones, Claudia
AU  - von Baer, Dietrich
AU  - Bautista-Baños, Silvia
AU  - Dublan Garcia, Octavio
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8891
AB  - The aim of this paper is to evaluate the physicochemical and microbiological properties of active thermoplastic starch-based materials. The extract obtained from grape cane waste was used as a source of stilbene bioactive components to enhance the functional properties of thermoplastic starch (TPS). The biomaterials were prepared by the compression molding technique and subjected to mechanical, thermal, antioxidant, and microbiological tests. The results showed that the addition of grape cane extract up to 15 wt% (TPS/WE15) did not significantly influence the thermal stability of obtained biomaterials, whereas mechanical resistance decreased. On the other side, among all tested pathogens, thermoplastic starch based materials showed antifungal activity toward Botrytis cinerea and antimicrobial activity toward Staphylococcus aureus, suggesting potential application in food packaging as an active biomaterial layer.
T2  - Molecules
T1  - Physical-Chemical Evaluation of Active Food Packaging Material Based on Thermoplastic Starch Loaded with Grape cane Extract
VL  - 25
IS  - 6
SP  - 1306
DO  - 10.3390/molecules25061306
ER  - 

@article{
author = "Díaz-Galindo, Edaena Pamela and Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Mardones, Claudia and von Baer, Dietrich and Bautista-Baños, Silvia and Dublan Garcia, Octavio",
year = "2020",
abstract = "The aim of this paper is to evaluate the physicochemical and microbiological properties of active thermoplastic starch-based materials. The extract obtained from grape cane waste was used as a source of stilbene bioactive components to enhance the functional properties of thermoplastic starch (TPS). The biomaterials were prepared by the compression molding technique and subjected to mechanical, thermal, antioxidant, and microbiological tests. The results showed that the addition of grape cane extract up to 15 wt% (TPS/WE15) did not significantly influence the thermal stability of obtained biomaterials, whereas mechanical resistance decreased. On the other side, among all tested pathogens, thermoplastic starch based materials showed antifungal activity toward Botrytis cinerea and antimicrobial activity toward Staphylococcus aureus, suggesting potential application in food packaging as an active biomaterial layer.",
journal = "Molecules",
title = "Physical-Chemical Evaluation of Active Food Packaging Material Based on Thermoplastic Starch Loaded with Grape cane Extract",
volume = "25",
number = "6",
pages = "1306",
doi = "10.3390/molecules25061306"
}

Díaz-Galindo, E. P., Nešić, A., Cabrera-Barjas, G., Mardones, C., von Baer, D., Bautista-Baños, S.,& Dublan Garcia, O.. (2020). Physical-Chemical Evaluation of Active Food Packaging Material Based on Thermoplastic Starch Loaded with Grape cane Extract. in Molecules, 25(6), 1306.
https://doi.org/10.3390/molecules25061306

Díaz-Galindo EP, Nešić A, Cabrera-Barjas G, Mardones C, von Baer D, Bautista-Baños S, Dublan Garcia O. Physical-Chemical Evaluation of Active Food Packaging Material Based on Thermoplastic Starch Loaded with Grape cane Extract. in Molecules. 2020;25(6):1306.
doi:10.3390/molecules25061306 .

Díaz-Galindo, Edaena Pamela, Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Mardones, Claudia, von Baer, Dietrich, Bautista-Baños, Silvia, Dublan Garcia, Octavio, "Physical-Chemical Evaluation of Active Food Packaging Material Based on Thermoplastic Starch Loaded with Grape cane Extract" in Molecules, 25, no. 6 (2020):1306,
https://doi.org/10.3390/molecules25061306 . .

TY  - JOUR
AU  - Diaz-Galindo, Edaena Pamela
AU  - Nešić, Aleksandra
AU  - Bautista-Baños, Silvia
AU  - Dublan García, Octavio
AU  - Cabrera-Barjas, Gustavo
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8944
AB  - Active packaging represents a large and diverse group of materials, with its main role being to prolong the shelf-life of food products. In this work, active biomaterials based on thermoplastic starch-containing cinnamon oil emulsions were prepared by the compression molding technique. The thermal, mechanical, and antifungal properties of obtained materials were evaluated. The results showed that the encapsulation of cinnamon oil emulsions did not influence the thermal stability of materials. Mechanical resistance to break was reduced by 27.4%, while elongation at break was increased by 44.0% by the addition of cinnamon oil emulsion. Moreover, the novel material provided a decrease in the growth rate of Botrytis cinerea by 66%, suggesting potential application in food packaging as an active biomaterial layer to hinder further contamination of fruits during the storage and transport period.
T2  - Foods
T1  - Corn-Starch-Based Materials Incorporated with Cinnamon Oil Emulsion: Physico-Chemical Characterization and Biological Activity
VL  - 9
IS  - 4
SP  - 475
DO  - 10.3390/foods9040475
ER  - 

@article{
author = "Diaz-Galindo, Edaena Pamela and Nešić, Aleksandra and Bautista-Baños, Silvia and Dublan García, Octavio and Cabrera-Barjas, Gustavo",
year = "2020",
abstract = "Active packaging represents a large and diverse group of materials, with its main role being to prolong the shelf-life of food products. In this work, active biomaterials based on thermoplastic starch-containing cinnamon oil emulsions were prepared by the compression molding technique. The thermal, mechanical, and antifungal properties of obtained materials were evaluated. The results showed that the encapsulation of cinnamon oil emulsions did not influence the thermal stability of materials. Mechanical resistance to break was reduced by 27.4%, while elongation at break was increased by 44.0% by the addition of cinnamon oil emulsion. Moreover, the novel material provided a decrease in the growth rate of Botrytis cinerea by 66%, suggesting potential application in food packaging as an active biomaterial layer to hinder further contamination of fruits during the storage and transport period.",
journal = "Foods",
title = "Corn-Starch-Based Materials Incorporated with Cinnamon Oil Emulsion: Physico-Chemical Characterization and Biological Activity",
volume = "9",
number = "4",
pages = "475",
doi = "10.3390/foods9040475"
}

Diaz-Galindo, E. P., Nešić, A., Bautista-Baños, S., Dublan García, O.,& Cabrera-Barjas, G.. (2020). Corn-Starch-Based Materials Incorporated with Cinnamon Oil Emulsion: Physico-Chemical Characterization and Biological Activity. in Foods, 9(4), 475.
https://doi.org/10.3390/foods9040475

Diaz-Galindo EP, Nešić A, Bautista-Baños S, Dublan García O, Cabrera-Barjas G. Corn-Starch-Based Materials Incorporated with Cinnamon Oil Emulsion: Physico-Chemical Characterization and Biological Activity. in Foods. 2020;9(4):475.
doi:10.3390/foods9040475 .

Diaz-Galindo, Edaena Pamela, Nešić, Aleksandra, Bautista-Baños, Silvia, Dublan García, Octavio, Cabrera-Barjas, Gustavo, "Corn-Starch-Based Materials Incorporated with Cinnamon Oil Emulsion: Physico-Chemical Characterization and Biological Activity" in Foods, 9, no. 4 (2020):475,
https://doi.org/10.3390/foods9040475 . .

TY  - JOUR
AU  - Segura, Rodrigo
AU  - Vásquez, Gustavo
AU  - Colson, Emmanuel
AU  - Gerbaux, Pascal
AU  - Frischmon, Caroline
AU  - Nešić, Aleksandra
AU  - García, Danni E.
AU  - Cabrera‐Barjas, Gustavo
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9069
AB  - BACKGROUND: Quinoa (Chenopodium quinoa Willd) is an Andean original pseudocereal with high nutritional value. During quinoa processing, large amounts of saponin-rich husks byproducts are obtained. Quinoa saponins, which are biologically active, could be used for various agriculture purposes. Silver nanoparticles have increasingly attracted attention for the management of crop diseases in agriculture. In this work, silver nanoparticles are synthesized by a sustainable and green method, using quinoa husk saponin extract (QE) to evaluate their potential for application in agriculture as biostimulants. RESULTS: Quinoa extract was obtained and characterized by liquid chromatography with tandem mass spectrometry (LC–MS/MS). Sixteen saponin congeners were successfully identified and quantified. The QE obtained was used as a reducing agent for silver ions to synthesize silver nanoparticles (QEAgNPs) under mild conditions. The morphology, particle size, and stability of Ag nanoparticles were investigated by transmission electron microscopy (TEM), ultraviolet–visible spectroscopy (UV-visible), energy-dispersive X-ray (EDS), zeta potential, and Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR). Ultraviolet–visible spectroscopy measurements confirmed the formation of silver nanoparticles in the presence of QE, with estimated particle sizes in a range between 5 and 50 nm. According to the zeta potential values, highly stable nanoparticles were formed. The QE and QEAgNPs (200–1000 μg/mL) were also tested in radish seed bioassay to evaluate their phytotoxicity. The seed germination assays revealed that QEAgNPs possessed a phytostimulant effect on radish seeds in a dose-dependent manner, and no phytotoxicity was observed for both QE and QEAgNPs. CONCLUSION: Silver nanoparticles obtained by a so-called ‘green’ method could be considered as good candidates for application in the agricultural sector for seed treatment, or as foliar sprays and plant-growth-promoters.
T2  - Journal of the Science of Food and Agriculture
T1  - Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa
VL  - 100
IS  - 13
SP  - 4987
EP  - 4994
DO  - 10.1002/jsfa.10529
ER  - 

@article{
author = "Segura, Rodrigo and Vásquez, Gustavo and Colson, Emmanuel and Gerbaux, Pascal and Frischmon, Caroline and Nešić, Aleksandra and García, Danni E. and Cabrera‐Barjas, Gustavo",
year = "2020",
abstract = "BACKGROUND: Quinoa (Chenopodium quinoa Willd) is an Andean original pseudocereal with high nutritional value. During quinoa processing, large amounts of saponin-rich husks byproducts are obtained. Quinoa saponins, which are biologically active, could be used for various agriculture purposes. Silver nanoparticles have increasingly attracted attention for the management of crop diseases in agriculture. In this work, silver nanoparticles are synthesized by a sustainable and green method, using quinoa husk saponin extract (QE) to evaluate their potential for application in agriculture as biostimulants. RESULTS: Quinoa extract was obtained and characterized by liquid chromatography with tandem mass spectrometry (LC–MS/MS). Sixteen saponin congeners were successfully identified and quantified. The QE obtained was used as a reducing agent for silver ions to synthesize silver nanoparticles (QEAgNPs) under mild conditions. The morphology, particle size, and stability of Ag nanoparticles were investigated by transmission electron microscopy (TEM), ultraviolet–visible spectroscopy (UV-visible), energy-dispersive X-ray (EDS), zeta potential, and Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR). Ultraviolet–visible spectroscopy measurements confirmed the formation of silver nanoparticles in the presence of QE, with estimated particle sizes in a range between 5 and 50 nm. According to the zeta potential values, highly stable nanoparticles were formed. The QE and QEAgNPs (200–1000 μg/mL) were also tested in radish seed bioassay to evaluate their phytotoxicity. The seed germination assays revealed that QEAgNPs possessed a phytostimulant effect on radish seeds in a dose-dependent manner, and no phytotoxicity was observed for both QE and QEAgNPs. CONCLUSION: Silver nanoparticles obtained by a so-called ‘green’ method could be considered as good candidates for application in the agricultural sector for seed treatment, or as foliar sprays and plant-growth-promoters.",
journal = "Journal of the Science of Food and Agriculture",
title = "Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa",
volume = "100",
number = "13",
pages = "4987-4994",
doi = "10.1002/jsfa.10529"
}

Segura, R., Vásquez, G., Colson, E., Gerbaux, P., Frischmon, C., Nešić, A., García, D. E.,& Cabrera‐Barjas, G.. (2020). Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa. in Journal of the Science of Food and Agriculture, 100(13), 4987-4994.
https://doi.org/10.1002/jsfa.10529

Segura R, Vásquez G, Colson E, Gerbaux P, Frischmon C, Nešić A, García DE, Cabrera‐Barjas G. Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa. in Journal of the Science of Food and Agriculture. 2020;100(13):4987-4994.
doi:10.1002/jsfa.10529 .

Segura, Rodrigo, Vásquez, Gustavo, Colson, Emmanuel, Gerbaux, Pascal, Frischmon, Caroline, Nešić, Aleksandra, García, Danni E., Cabrera‐Barjas, Gustavo, "Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa" in Journal of the Science of Food and Agriculture, 100, no. 13 (2020):4987-4994,
https://doi.org/10.1002/jsfa.10529 . .

TY  - JOUR
AU  - Díaz-Galindo, E.P.
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Dublan-García, Octavio
AU  - Ventura-Aguilar, Rosa Isela
AU  - Vázquez-Armenta, Francisco Javier
AU  - Aguilar-Montes de Oca, Saúl 
AU  - Mardones, Claudia
AU  - Ayala-Zavala, Jesus Fernando
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9786
AB  - The aim of this study was evaluation of the physico-chemical properties and adhesion of microorganisms on poly(lactic acid) (PLA)-based films loaded with grapevine cane extract (5–15 wt%). The films were processed in a compression molding machine and characterized by mechanical, thermal, water vapor barrier and microbiological tests. The best physical-chemical properties for PLA film containing 10 wt% of extract were obtained. The addition of 10 wt% of extract into PLA films led to decrease of tensile strength for 52% and increase in elongation at break for 30%. The water vapor barrier of this film formulation was enhanced for 55%. All films showed thermal stability up to 300◦C. The low release of the active compounds from films negatively influenced their antimicrobial and antifungal activity. Botrytis cinerea growth inhibition onto PLA containing extracts (PLA-E) films was in the range between 15 and 35%. On the other side, PLA/extract films exhibited the antiadhesive properties against Pseudomonas aeruginosa, Pectobacterium carotovorum, Saccharomyces pastorianus, and Listeria monocytogenes, which could imply their potential to be used as sustainable food packaging materials for preventing microbial contamination of food. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
T2  - Polymers
T2  - Polymers
T1  - Physico-chemical and antiadhesive properties of poly(Lactic acid)/grapevine cane extract films against food pathogenic microorganisms
VL  - 12
IS  - 12
SP  - 1
EP  - 15
DO  - 10.3390/polym12122967
ER  - 

@article{
author = "Díaz-Galindo, E.P. and Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Dublan-García, Octavio and Ventura-Aguilar, Rosa Isela and Vázquez-Armenta, Francisco Javier and Aguilar-Montes de Oca, Saúl  and Mardones, Claudia and Ayala-Zavala, Jesus Fernando",
year = "2020",
abstract = "The aim of this study was evaluation of the physico-chemical properties and adhesion of microorganisms on poly(lactic acid) (PLA)-based films loaded with grapevine cane extract (5–15 wt%). The films were processed in a compression molding machine and characterized by mechanical, thermal, water vapor barrier and microbiological tests. The best physical-chemical properties for PLA film containing 10 wt% of extract were obtained. The addition of 10 wt% of extract into PLA films led to decrease of tensile strength for 52% and increase in elongation at break for 30%. The water vapor barrier of this film formulation was enhanced for 55%. All films showed thermal stability up to 300◦C. The low release of the active compounds from films negatively influenced their antimicrobial and antifungal activity. Botrytis cinerea growth inhibition onto PLA containing extracts (PLA-E) films was in the range between 15 and 35%. On the other side, PLA/extract films exhibited the antiadhesive properties against Pseudomonas aeruginosa, Pectobacterium carotovorum, Saccharomyces pastorianus, and Listeria monocytogenes, which could imply their potential to be used as sustainable food packaging materials for preventing microbial contamination of food. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
journal = "Polymers, Polymers",
title = "Physico-chemical and antiadhesive properties of poly(Lactic acid)/grapevine cane extract films against food pathogenic microorganisms",
volume = "12",
number = "12",
pages = "1-15",
doi = "10.3390/polym12122967"
}

Díaz-Galindo, E.P., Nešić, A., Cabrera-Barjas, G., Dublan-García, O., Ventura-Aguilar, R. I., Vázquez-Armenta, F. J., Aguilar-Montes de Oca, S., Mardones, C.,& Ayala-Zavala, J. F.. (2020). Physico-chemical and antiadhesive properties of poly(Lactic acid)/grapevine cane extract films against food pathogenic microorganisms. in Polymers, 12(12), 1-15.
https://doi.org/10.3390/polym12122967

Díaz-Galindo E, Nešić A, Cabrera-Barjas G, Dublan-García O, Ventura-Aguilar RI, Vázquez-Armenta FJ, Aguilar-Montes de Oca S, Mardones C, Ayala-Zavala JF. Physico-chemical and antiadhesive properties of poly(Lactic acid)/grapevine cane extract films against food pathogenic microorganisms. in Polymers. 2020;12(12):1-15.
doi:10.3390/polym12122967 .

Díaz-Galindo, E.P., Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Dublan-García, Octavio, Ventura-Aguilar, Rosa Isela, Vázquez-Armenta, Francisco Javier, Aguilar-Montes de Oca, Saúl , Mardones, Claudia, Ayala-Zavala, Jesus Fernando, "Physico-chemical and antiadhesive properties of poly(Lactic acid)/grapevine cane extract films against food pathogenic microorganisms" in Polymers, 12, no. 12 (2020):1-15,
https://doi.org/10.3390/polym12122967 . .

TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Gallardo, Francisco
AU  - Nešić, Aleksandra
AU  - Taboada, Edelio
AU  - Marican, Adolfo
AU  - Mirabal-Gallardo, Yaneris
AU  - Avila-Salas, Fabian
AU  - Delgado, Nacarid
AU  - de Armas-Ricard, Merly
AU  - Valdes, Oscar
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10701
AB  - In this work, Aspergillus niger and Fusarium culmorum cell wall by-products were chosen as microbial sources for chitin and chitosan production. Both polysaccharides were characterized by FTIR and 13C-CPMAS NMR, but GPC analysis was only performed for chitosan. SEM-EDX analysis was performed to fungal chitosan loaded with metal ions. Chitosan extracted from both fungus had low to medium molecular weight (Mw) and degree of deacetylations (DD) ranging from 65.7-83.3%. Fungal chitosan samples were intended to be used for bioremediation applications. For this purpose, two independent absorption experiments regarding pesticide Dimethoate (DM) and heavy metal ions (Al(III), As(III), Cd(II), Cu(II), Mg(II), Mn(II), Pb(II), Zn(II), Fe(II)) in a complex mixture were carried out. An experimental design considering the solution pH, contact time and chitosan physicochemical properties (DD) were performed. The highest percentage of dimethoate pesticide and selected metal ions absorption was obtained with highest DD chitosan, the contact time of 24 h, pH 6 for metals and pH 4 for pesticide, repectively. Molecular dynamics simulation studies allowed to analyze at the molecular level the chitosan-DM interaction. A higher number of h-bonds were identified as the main interactions that stabilize the affinity of the chitosan-DM complexes. Based on our results, we suggest the use of a multipurpose fungal chitosan system for water bioremediation.
T2  - Journal of Environmental Chemical Engineering
T1  - Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions
VL  - 8
IS  - 5
SP  - 104355
DO  - 10.1016/j.jece.2020.104355
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Gallardo, Francisco and Nešić, Aleksandra and Taboada, Edelio and Marican, Adolfo and Mirabal-Gallardo, Yaneris and Avila-Salas, Fabian and Delgado, Nacarid and de Armas-Ricard, Merly and Valdes, Oscar",
year = "2020",
abstract = "In this work, Aspergillus niger and Fusarium culmorum cell wall by-products were chosen as microbial sources for chitin and chitosan production. Both polysaccharides were characterized by FTIR and 13C-CPMAS NMR, but GPC analysis was only performed for chitosan. SEM-EDX analysis was performed to fungal chitosan loaded with metal ions. Chitosan extracted from both fungus had low to medium molecular weight (Mw) and degree of deacetylations (DD) ranging from 65.7-83.3%. Fungal chitosan samples were intended to be used for bioremediation applications. For this purpose, two independent absorption experiments regarding pesticide Dimethoate (DM) and heavy metal ions (Al(III), As(III), Cd(II), Cu(II), Mg(II), Mn(II), Pb(II), Zn(II), Fe(II)) in a complex mixture were carried out. An experimental design considering the solution pH, contact time and chitosan physicochemical properties (DD) were performed. The highest percentage of dimethoate pesticide and selected metal ions absorption was obtained with highest DD chitosan, the contact time of 24 h, pH 6 for metals and pH 4 for pesticide, repectively. Molecular dynamics simulation studies allowed to analyze at the molecular level the chitosan-DM interaction. A higher number of h-bonds were identified as the main interactions that stabilize the affinity of the chitosan-DM complexes. Based on our results, we suggest the use of a multipurpose fungal chitosan system for water bioremediation.",
journal = "Journal of Environmental Chemical Engineering",
title = "Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions",
volume = "8",
number = "5",
pages = "104355",
doi = "10.1016/j.jece.2020.104355"
}

Cabrera-Barjas, G., Gallardo, F., Nešić, A., Taboada, E., Marican, A., Mirabal-Gallardo, Y., Avila-Salas, F., Delgado, N., de Armas-Ricard, M.,& Valdes, O.. (2020). Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions. in Journal of Environmental Chemical Engineering, 8(5), 104355.
https://doi.org/10.1016/j.jece.2020.104355

Cabrera-Barjas G, Gallardo F, Nešić A, Taboada E, Marican A, Mirabal-Gallardo Y, Avila-Salas F, Delgado N, de Armas-Ricard M, Valdes O. Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions. in Journal of Environmental Chemical Engineering. 2020;8(5):104355.
doi:10.1016/j.jece.2020.104355 .

Cabrera-Barjas, Gustavo, Gallardo, Francisco, Nešić, Aleksandra, Taboada, Edelio, Marican, Adolfo, Mirabal-Gallardo, Yaneris, Avila-Salas, Fabian, Delgado, Nacarid, de Armas-Ricard, Merly, Valdes, Oscar, "Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions" in Journal of Environmental Chemical Engineering, 8, no. 5 (2020):104355,
https://doi.org/10.1016/j.jece.2020.104355 . .

TY  - JOUR
AU  - Bernabé, Patricia
AU  - Becherán, Liliam
AU  - Cabrera-Barjas, Gustavo
AU  - Nešić, Aleksandra
AU  - Alburquenque, Claudio
AU  - Tapia, Cecilia V.
AU  - Taboada, Edelio
AU  - Alderete, Joel
AU  - De Los Ríos, Patricio
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10702
AB  - In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time. α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0–10.4% (w/w). Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes. Aegla cholchol exoskeleton, chitin and chitosans were characterized by FTIR, TGA, and SEM. Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy. Chitosan molecular weight determinations were carried out by GPC. The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65–201 kDa. The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast. Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis. Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
T2  - International Journal of Biological Macromolecules
T1  - Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp
VL  - 149
SP  - 962
EP  - 975
DO  - 10.1016/j.ijbiomac.2020.01.126
ER  - 

@article{
author = "Bernabé, Patricia and Becherán, Liliam and Cabrera-Barjas, Gustavo and Nešić, Aleksandra and Alburquenque, Claudio and Tapia, Cecilia V. and Taboada, Edelio and Alderete, Joel and De Los Ríos, Patricio",
year = "2020",
abstract = "In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time. α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0–10.4% (w/w). Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes. Aegla cholchol exoskeleton, chitin and chitosans were characterized by FTIR, TGA, and SEM. Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy. Chitosan molecular weight determinations were carried out by GPC. The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65–201 kDa. The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast. Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis. Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.",
journal = "International Journal of Biological Macromolecules",
title = "Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp",
volume = "149",
pages = "962-975",
doi = "10.1016/j.ijbiomac.2020.01.126"
}

Bernabé, P., Becherán, L., Cabrera-Barjas, G., Nešić, A., Alburquenque, C., Tapia, C. V., Taboada, E., Alderete, J.,& De Los Ríos, P.. (2020). Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp. in International Journal of Biological Macromolecules, 149, 962-975.
https://doi.org/10.1016/j.ijbiomac.2020.01.126

Bernabé P, Becherán L, Cabrera-Barjas G, Nešić A, Alburquenque C, Tapia CV, Taboada E, Alderete J, De Los Ríos P. Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp. in International Journal of Biological Macromolecules. 2020;149:962-975.
doi:10.1016/j.ijbiomac.2020.01.126 .

Bernabé, Patricia, Becherán, Liliam, Cabrera-Barjas, Gustavo, Nešić, Aleksandra, Alburquenque, Claudio, Tapia, Cecilia V., Taboada, Edelio, Alderete, Joel, De Los Ríos, Patricio, "Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp" in International Journal of Biological Macromolecules, 149 (2020):962-975,
https://doi.org/10.1016/j.ijbiomac.2020.01.126 . .

TY  - CHAP
AU  - Nešić, Aleksandra
AU  - Castillo, Catalina
AU  - Castaño, Patricia
AU  - Cabrera-Barjas, Gustavo
AU  - Serrano, Jesus
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10718
AB  - This chapter summarizes the current state of bio-based materials in food packaging sector. Driven by the increasing needs of sustainability, huge effort has been given to development of new materials that are obtained from renewable sources and can potentially replace petroleum-derived materials on market. A range of main bio-based polymers targeted for food packaging industry are presented focusing on structural properties of bio-based polymers, general processing techniques, main limitations of these materials, and current state on market. Moreover, the chapter examines the new technological approaches in upgrading the properties of bio-based packaging materials to a commercial application, together with potential solutions, as well as discusses the major industry players who are bringing these materials to the market.
T2  - Biobased Products and Industries
T1  - Bio-based packaging materials
SP  - 279
EP  - 309
DO  - 10.1016/B978-0-12-818493-6.00008-7
ER  - 

@inbook{
author = "Nešić, Aleksandra and Castillo, Catalina and Castaño, Patricia and Cabrera-Barjas, Gustavo and Serrano, Jesus",
year = "2020",
abstract = "This chapter summarizes the current state of bio-based materials in food packaging sector. Driven by the increasing needs of sustainability, huge effort has been given to development of new materials that are obtained from renewable sources and can potentially replace petroleum-derived materials on market. A range of main bio-based polymers targeted for food packaging industry are presented focusing on structural properties of bio-based polymers, general processing techniques, main limitations of these materials, and current state on market. Moreover, the chapter examines the new technological approaches in upgrading the properties of bio-based packaging materials to a commercial application, together with potential solutions, as well as discusses the major industry players who are bringing these materials to the market.",
journal = "Biobased Products and Industries",
booktitle = "Bio-based packaging materials",
pages = "279-309",
doi = "10.1016/B978-0-12-818493-6.00008-7"
}

Nešić, A., Castillo, C., Castaño, P., Cabrera-Barjas, G.,& Serrano, J.. (2020). Bio-based packaging materials. in Biobased Products and Industries, 279-309.
https://doi.org/10.1016/B978-0-12-818493-6.00008-7

Nešić A, Castillo C, Castaño P, Cabrera-Barjas G, Serrano J. Bio-based packaging materials. in Biobased Products and Industries. 2020;:279-309.
doi:10.1016/B978-0-12-818493-6.00008-7 .

Nešić, Aleksandra, Castillo, Catalina, Castaño, Patricia, Cabrera-Barjas, Gustavo, Serrano, Jesus, "Bio-based packaging materials" in Biobased Products and Industries (2020):279-309,
https://doi.org/10.1016/B978-0-12-818493-6.00008-7 . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Dimitrijević-Branković, Suzana I.
AU  - Davidović, Slađana Z.
AU  - Radovanović, Neda
AU  - Delattre, Cédric
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8800
AB  - The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product’s carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.
T2  - Molecules
T1  - Prospect of Polysaccharide-Based Materials as Advanced Food Packaging
VL  - 25
IS  - 1
SP  - 135
DO  - 10.3390/molecules25010135
ER  - 

@article{
author = "Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Dimitrijević-Branković, Suzana I. and Davidović, Slađana Z. and Radovanović, Neda and Delattre, Cédric",
year = "2019",
abstract = "The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product’s carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.",
journal = "Molecules",
title = "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging",
volume = "25",
number = "1",
pages = "135",
doi = "10.3390/molecules25010135"
}

Nešić, A., Cabrera-Barjas, G., Dimitrijević-Branković, S. I., Davidović, S. Z., Radovanović, N.,& Delattre, C.. (2019). Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules, 25(1), 135.
https://doi.org/10.3390/molecules25010135

Nešić A, Cabrera-Barjas G, Dimitrijević-Branković SI, Davidović SZ, Radovanović N, Delattre C. Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules. 2019;25(1):135.
doi:10.3390/molecules25010135 .

Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Dimitrijević-Branković, Suzana I., Davidović, Slađana Z., Radovanović, Neda, Delattre, Cédric, "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging" in Molecules, 25, no. 1 (2019):135,
https://doi.org/10.3390/molecules25010135 . .