TY  - JOUR
AU  - Nikezić, Gordana S.
AU  - Horvat, Anica
AU  - Nedeljković, N.
AU  - Todorović, S.
AU  - Nikolić, Vladimir M.
AU  - Kanazir, Dušan T.
AU  - Vujisić Lj.
AU  - Kopečni Miroslav
PY  - 1998
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2146
AB  - The neurotoxicity of pyridine and urea was investigated in respect to their ability to alter the activity of synaptosomal membrane Na+/K+-ATPase and Mg2+-ATPase. In vitro treatment with pyridine and urea stimulated Na+/K+-ATPase activity in a dose-dependent manner up to 40% and 60%, respectively. Mg2+-ATPase activity increased up to 40% after pyridine treatment, while urea had no effect at all. The neuroactive potencies of pyridine and urea were evaluated by estimating parameters K-m and Delta V-max for enzyme stimulation, as well as Hill coefficient to estimate the levels of cooperativity for pyridine and urea binding. The results suggest that pyridine stimulates both enzymes, probably by interacting with some neuronal membrane components, and altering the lipid micro-environment of the ATPases. In contrast, urea stimulates the Na+/K+-ATPase only, assumingly by acting on it directly or via some other regulatory mechanism. Stimulation of Na+/K+-ATPase and Mg2+-ATPase by the substances tested and subsequent alteration of neuronal cell functioning could contribute to the CNS dysfunction upon chronic exposure to pyridine and urea.
T2  - General Physiology and Biophysics
T1  - Influence of pyridine and urea on the rat brain ATPase activity
VL  - 17
IS  - 1
SP  - 15
EP  - 23
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2146
ER  - 

@article{
author = "Nikezić, Gordana S. and Horvat, Anica and Nedeljković, N. and Todorović, S. and Nikolić, Vladimir M. and Kanazir, Dušan T. and Vujisić Lj. and Kopečni Miroslav",
year = "1998",
abstract = "The neurotoxicity of pyridine and urea was investigated in respect to their ability to alter the activity of synaptosomal membrane Na+/K+-ATPase and Mg2+-ATPase. In vitro treatment with pyridine and urea stimulated Na+/K+-ATPase activity in a dose-dependent manner up to 40% and 60%, respectively. Mg2+-ATPase activity increased up to 40% after pyridine treatment, while urea had no effect at all. The neuroactive potencies of pyridine and urea were evaluated by estimating parameters K-m and Delta V-max for enzyme stimulation, as well as Hill coefficient to estimate the levels of cooperativity for pyridine and urea binding. The results suggest that pyridine stimulates both enzymes, probably by interacting with some neuronal membrane components, and altering the lipid micro-environment of the ATPases. In contrast, urea stimulates the Na+/K+-ATPase only, assumingly by acting on it directly or via some other regulatory mechanism. Stimulation of Na+/K+-ATPase and Mg2+-ATPase by the substances tested and subsequent alteration of neuronal cell functioning could contribute to the CNS dysfunction upon chronic exposure to pyridine and urea.",
journal = "General Physiology and Biophysics",
title = "Influence of pyridine and urea on the rat brain ATPase activity",
volume = "17",
number = "1",
pages = "15-23",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2146"
}

Nikezić, G. S., Horvat, A., Nedeljković, N., Todorović, S., Nikolić, V. M., Kanazir, D. T., Vujisić Lj.,& Kopečni Miroslav. (1998). Influence of pyridine and urea on the rat brain ATPase activity. in General Physiology and Biophysics, 17(1), 15-23.
https://hdl.handle.net/21.15107/rcub_vinar_2146

Nikezić GS, Horvat A, Nedeljković N, Todorović S, Nikolić VM, Kanazir DT, Vujisić Lj., Kopečni Miroslav. Influence of pyridine and urea on the rat brain ATPase activity. in General Physiology and Biophysics. 1998;17(1):15-23.
https://hdl.handle.net/21.15107/rcub_vinar_2146 .

Nikezić, Gordana S., Horvat, Anica, Nedeljković, N., Todorović, S., Nikolić, Vladimir M., Kanazir, Dušan T., Vujisić Lj., Kopečni Miroslav, "Influence of pyridine and urea on the rat brain ATPase activity" in General Physiology and Biophysics, 17, no. 1 (1998):15-23,
https://hdl.handle.net/21.15107/rcub_vinar_2146 .