TY  - JOUR
AU  - Ranjani, Singaraj
AU  - Kowshik, Jaganathan
AU  - Sophia, Josephraj
AU  - Nivetha, Ramesh
AU  - Baba, Abdul B
AU  - Veeravarmal, Veeran
AU  - Joksić, Gordana
AU  - Rutqvist, Lars E
AU  - Nilsson, Robert
AU  - Nagini, Siddavaram
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8943
AB  - Background and Objectives: The present study was undertaken to ascertain whether the modulatory effects of blueberries on cell proliferation induced by Swedish snus in the rat forestomach epithelium is mediated via abrogation of the PI3K/Akt/NFκB signaling axis that regulates cell fate decision. Methods: The transcript and protein expression of genes involved in cell cycle progression and apoptosis, as well as canonical PI3K/Akt/NF-κB signaling pathways, were analyzed by qRT-PCR, immunoblotting and ELISA. Expression profiling of noncoding RNAs (ncRNAs) that influence PI3K/Akt/NF-κB signaling was undertaken. TUNEL assay was performed using flow cytometry. Results: Administration of snus induced basal cell hyperplasia in the rat forestomach with increased cell proliferation and inhibition of apoptosis. This was associated with the activation of PI3K/Akt/NFκB signaling. Coadministration of blueberries significantly suppressed snus-induced hyperplasia. Analysis of the molecular mechanisms revealed that blueberries suppress the phosphorylation of Akt, NF-κB and IKKβ, prevent nuclear translocation of NF-κB and modulate the expression of microRNAs that influence PI3K/Akt/NF-κB signaling. Conclusion: Taken together, the results of the current study provide compelling evidence that blueberries exert significant protective effects against snus-induced soft tissue changes in the rat forestomach epithelium mediated by inhibiting key molecular players in the PI3K/Akt/NF-κB signaling axis. Long-term studies on the impact of snus exposure on various cellular processes, signaling pathways, and the interplay between genetic and epigenetic mechanisms are however warranted. The results of this investigation may contribute to the development of protection against soft tissue changes induced by smokeless tobacco in the human oral cavity.
T2  - Anti-Cancer Agents in Medicinal Chemistry
T1  - Activation of PI3K/Akt/NF-kB Signaling Mediates Swedish Snus Induced Proliferation and Apoptosis Evasion in the Rat Forestomach: Modulation by Blueberry
VL  - 20
IS  - 1
SP  - 59
EP  - 69
DO  - 10.2174/1871520619666191024115738
ER  - 

@article{
author = "Ranjani, Singaraj and Kowshik, Jaganathan and Sophia, Josephraj and Nivetha, Ramesh and Baba, Abdul B and Veeravarmal, Veeran and Joksić, Gordana and Rutqvist, Lars E and Nilsson, Robert and Nagini, Siddavaram",
year = "2020",
abstract = "Background and Objectives: The present study was undertaken to ascertain whether the modulatory effects of blueberries on cell proliferation induced by Swedish snus in the rat forestomach epithelium is mediated via abrogation of the PI3K/Akt/NFκB signaling axis that regulates cell fate decision. Methods: The transcript and protein expression of genes involved in cell cycle progression and apoptosis, as well as canonical PI3K/Akt/NF-κB signaling pathways, were analyzed by qRT-PCR, immunoblotting and ELISA. Expression profiling of noncoding RNAs (ncRNAs) that influence PI3K/Akt/NF-κB signaling was undertaken. TUNEL assay was performed using flow cytometry. Results: Administration of snus induced basal cell hyperplasia in the rat forestomach with increased cell proliferation and inhibition of apoptosis. This was associated with the activation of PI3K/Akt/NFκB signaling. Coadministration of blueberries significantly suppressed snus-induced hyperplasia. Analysis of the molecular mechanisms revealed that blueberries suppress the phosphorylation of Akt, NF-κB and IKKβ, prevent nuclear translocation of NF-κB and modulate the expression of microRNAs that influence PI3K/Akt/NF-κB signaling. Conclusion: Taken together, the results of the current study provide compelling evidence that blueberries exert significant protective effects against snus-induced soft tissue changes in the rat forestomach epithelium mediated by inhibiting key molecular players in the PI3K/Akt/NF-κB signaling axis. Long-term studies on the impact of snus exposure on various cellular processes, signaling pathways, and the interplay between genetic and epigenetic mechanisms are however warranted. The results of this investigation may contribute to the development of protection against soft tissue changes induced by smokeless tobacco in the human oral cavity.",
journal = "Anti-Cancer Agents in Medicinal Chemistry",
title = "Activation of PI3K/Akt/NF-kB Signaling Mediates Swedish Snus Induced Proliferation and Apoptosis Evasion in the Rat Forestomach: Modulation by Blueberry",
volume = "20",
number = "1",
pages = "59-69",
doi = "10.2174/1871520619666191024115738"
}

Ranjani, S., Kowshik, J., Sophia, J., Nivetha, R., Baba, A. B., Veeravarmal, V., Joksić, G., Rutqvist, L. E., Nilsson, R.,& Nagini, S.. (2020). Activation of PI3K/Akt/NF-kB Signaling Mediates Swedish Snus Induced Proliferation and Apoptosis Evasion in the Rat Forestomach: Modulation by Blueberry. in Anti-Cancer Agents in Medicinal Chemistry, 20(1), 59-69.
https://doi.org/10.2174/1871520619666191024115738

Ranjani S, Kowshik J, Sophia J, Nivetha R, Baba AB, Veeravarmal V, Joksić G, Rutqvist LE, Nilsson R, Nagini S. Activation of PI3K/Akt/NF-kB Signaling Mediates Swedish Snus Induced Proliferation and Apoptosis Evasion in the Rat Forestomach: Modulation by Blueberry. in Anti-Cancer Agents in Medicinal Chemistry. 2020;20(1):59-69.
doi:10.2174/1871520619666191024115738 .

Ranjani, Singaraj, Kowshik, Jaganathan, Sophia, Josephraj, Nivetha, Ramesh, Baba, Abdul B, Veeravarmal, Veeran, Joksić, Gordana, Rutqvist, Lars E, Nilsson, Robert, Nagini, Siddavaram, "Activation of PI3K/Akt/NF-kB Signaling Mediates Swedish Snus Induced Proliferation and Apoptosis Evasion in the Rat Forestomach: Modulation by Blueberry" in Anti-Cancer Agents in Medicinal Chemistry, 20, no. 1 (2020):59-69,
https://doi.org/10.2174/1871520619666191024115738 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Rutqvist, Lars Erik
AU  - Mićić, Mileva
AU  - Filipović Tričković, Jelena G.
AU  - Nilsson, Robert
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0273230019300601
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8080
AB  - Long term exposure to oral smokeless tobacco may induce lesions in the oral cavity characterized by a hyperplastic epithelium. The possible role of nicotine and the physical properties of oral tobacco for developing these lesions, as well as of dysplasia and neoplasia is unclear. Low nitrosamine Swedish snus as well as non-genotoxic butylated hydroxyanisole induces increased cellular proliferation in the rat forestomach epithelia. Using this model, we report here on the effects of nicotine, pH, and particle size. Snus with different properties had no impact on oxidative stress as determined by 8-oxo-7,8-dihydro-2′-deoxyguanosine, or on interleukin IL-1b. Whereas BHA boosted IL-6, probably due to the presence of nicotine. there was no significant enhancement of cell divisions with increasing particle size, although in individual samples the variations in proliferation rates increased greatly with increasing particle size. Conforming to human experience, the enhanced cell proliferation caused by snus was found to be completely reversible. A cacao bean extract had a protective action similar to that previously found for blueberries. The main cause of the observed tobacco induced cell proliferation could be mechanical irritation, possibly in combination with nicotine, whereas within the studied range, pH did not affect the rate of cell division. © 2019
T2  - Regulatory Toxicology and Pharmacology
T1  - Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus)
VL  - 104
SP  - 21
EP  - 28
DO  - 10.1016/j.yrtph.2019.02.015
ER  - 

@article{
author = "Joksić, Gordana and Rutqvist, Lars Erik and Mićić, Mileva and Filipović Tričković, Jelena G. and Nilsson, Robert",
year = "2019",
abstract = "Long term exposure to oral smokeless tobacco may induce lesions in the oral cavity characterized by a hyperplastic epithelium. The possible role of nicotine and the physical properties of oral tobacco for developing these lesions, as well as of dysplasia and neoplasia is unclear. Low nitrosamine Swedish snus as well as non-genotoxic butylated hydroxyanisole induces increased cellular proliferation in the rat forestomach epithelia. Using this model, we report here on the effects of nicotine, pH, and particle size. Snus with different properties had no impact on oxidative stress as determined by 8-oxo-7,8-dihydro-2′-deoxyguanosine, or on interleukin IL-1b. Whereas BHA boosted IL-6, probably due to the presence of nicotine. there was no significant enhancement of cell divisions with increasing particle size, although in individual samples the variations in proliferation rates increased greatly with increasing particle size. Conforming to human experience, the enhanced cell proliferation caused by snus was found to be completely reversible. A cacao bean extract had a protective action similar to that previously found for blueberries. The main cause of the observed tobacco induced cell proliferation could be mechanical irritation, possibly in combination with nicotine, whereas within the studied range, pH did not affect the rate of cell division. © 2019",
journal = "Regulatory Toxicology and Pharmacology",
title = "Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus)",
volume = "104",
pages = "21-28",
doi = "10.1016/j.yrtph.2019.02.015"
}

Joksić, G., Rutqvist, L. E., Mićić, M., Filipović Tričković, J. G.,& Nilsson, R.. (2019). Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus). in Regulatory Toxicology and Pharmacology, 104, 21-28.
https://doi.org/10.1016/j.yrtph.2019.02.015

Joksić G, Rutqvist LE, Mićić M, Filipović Tričković JG, Nilsson R. Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus). in Regulatory Toxicology and Pharmacology. 2019;104:21-28.
doi:10.1016/j.yrtph.2019.02.015 .

Joksić, Gordana, Rutqvist, Lars Erik, Mićić, Mileva, Filipović Tričković, Jelena G., Nilsson, Robert, "Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus)" in Regulatory Toxicology and Pharmacology, 104 (2019):21-28,
https://doi.org/10.1016/j.yrtph.2019.02.015 . .

TY  - JOUR
AU  - Nilsson, Robert
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1687
AB  - To support risk management decisions, information from different fields has been integrated in this presentation to provide a realistic quantitative cancer risk assessment of smokeless tobacco. Smoking among Swedish men is currently below 10%, while about 20% use a special smokeless tobacco (snus) as a substitute for cigarettes. Epidemiological data and molecular biomarkers demonstrate that rodent bioassays with tobacco specific nitrosamines (TSNA) overestimate cancer risk from snus by more than one order of magnitude. The underlying reasons are discussed. DNA damage constitutes a necessary, although not sufficient prerequisite for cancer initiation. Individuals who have not used tobacco exhibit DNA lesions identical with those induced by TSNA. No increase above this adduct background can be shown from snus, and extensive epidemiological studies in Sweden have failed to demonstrate elevated cancer risks even in long term users. A bench mark for acceptable risk of 1/10(6) derived from rodent data has been suggested when regulating snus. By relating similarly derived estimates for some food contaminants, the implementation even of a limit of 1/10(4) may be unrealistic. The management of smokeless tobacco products has rarely been based on a scientifically sound risk assessment, where attention is given to the outstandingly higher hazards associated with smoking. (C) 2017 Elsevier Inc. All rights reserved.
T2  - Regulatory Toxicology and Pharmacology
T1  - Use of rodent data for cancer risk assessment of smokeless tobacco in the regulatory context
VL  - 88
SP  - 338
EP  - 348
DO  - 10.1016/j.yrtph.2017.06.005
ER  - 

@article{
author = "Nilsson, Robert",
year = "2017",
abstract = "To support risk management decisions, information from different fields has been integrated in this presentation to provide a realistic quantitative cancer risk assessment of smokeless tobacco. Smoking among Swedish men is currently below 10%, while about 20% use a special smokeless tobacco (snus) as a substitute for cigarettes. Epidemiological data and molecular biomarkers demonstrate that rodent bioassays with tobacco specific nitrosamines (TSNA) overestimate cancer risk from snus by more than one order of magnitude. The underlying reasons are discussed. DNA damage constitutes a necessary, although not sufficient prerequisite for cancer initiation. Individuals who have not used tobacco exhibit DNA lesions identical with those induced by TSNA. No increase above this adduct background can be shown from snus, and extensive epidemiological studies in Sweden have failed to demonstrate elevated cancer risks even in long term users. A bench mark for acceptable risk of 1/10(6) derived from rodent data has been suggested when regulating snus. By relating similarly derived estimates for some food contaminants, the implementation even of a limit of 1/10(4) may be unrealistic. The management of smokeless tobacco products has rarely been based on a scientifically sound risk assessment, where attention is given to the outstandingly higher hazards associated with smoking. (C) 2017 Elsevier Inc. All rights reserved.",
journal = "Regulatory Toxicology and Pharmacology",
title = "Use of rodent data for cancer risk assessment of smokeless tobacco in the regulatory context",
volume = "88",
pages = "338-348",
doi = "10.1016/j.yrtph.2017.06.005"
}

Nilsson, R.. (2017). Use of rodent data for cancer risk assessment of smokeless tobacco in the regulatory context. in Regulatory Toxicology and Pharmacology, 88, 338-348.
https://doi.org/10.1016/j.yrtph.2017.06.005

Nilsson R. Use of rodent data for cancer risk assessment of smokeless tobacco in the regulatory context. in Regulatory Toxicology and Pharmacology. 2017;88:338-348.
doi:10.1016/j.yrtph.2017.06.005 .

Nilsson, Robert, "Use of rodent data for cancer risk assessment of smokeless tobacco in the regulatory context" in Regulatory Toxicology and Pharmacology, 88 (2017):338-348,
https://doi.org/10.1016/j.yrtph.2017.06.005 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Mićić, Mileva
AU  - Filipović, Jelena G.
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Calija, Bojan
AU  - Valenta-Šobot, Ana
AU  - Demajo, Miroslav
AU  - Nilsson, Robert
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1584
AB  - The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
T2  - Acta Veterinaria, Beograd
T1  - Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach
VL  - 67
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1515/acve-2017-0001
ER  - 

@article{
author = "Joksić, Gordana and Mićić, Mileva and Filipović, Jelena G. and Drakulić, Dunja R. and Stanojlović, Miloš R. and Calija, Bojan and Valenta-Šobot, Ana and Demajo, Miroslav and Nilsson, Robert",
year = "2017",
abstract = "The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.",
journal = "Acta Veterinaria, Beograd",
title = "Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach",
volume = "67",
number = "1",
pages = "1-10",
doi = "10.1515/acve-2017-0001"
}

Joksić, G., Mićić, M., Filipović, J. G., Drakulić, D. R., Stanojlović, M. R., Calija, B., Valenta-Šobot, A., Demajo, M.,& Nilsson, R.. (2017). Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach. in Acta Veterinaria, Beograd, 67(1), 1-10.
https://doi.org/10.1515/acve-2017-0001

Joksić G, Mićić M, Filipović JG, Drakulić DR, Stanojlović MR, Calija B, Valenta-Šobot A, Demajo M, Nilsson R. Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach. in Acta Veterinaria, Beograd. 2017;67(1):1-10.
doi:10.1515/acve-2017-0001 .

Joksić, Gordana, Mićić, Mileva, Filipović, Jelena G., Drakulić, Dunja R., Stanojlović, Miloš R., Calija, Bojan, Valenta-Šobot, Ana, Demajo, Miroslav, Nilsson, Robert, "Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach" in Acta Veterinaria, Beograd, 67, no. 1 (2017):1-10,
https://doi.org/10.1515/acve-2017-0001 . .

TY  - JOUR
AU  - Nilsson, Robert
AU  - Mićić, Mileva
AU  - Filipović, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Joksić, Gordana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1058
AB  - The aim of this study was to identify palatable additives which have a significant protective action against soft tissue changes in the oral cavity caused by Swedish smokeless tobacco (snus), and that satisfy existing legal requirements. Although the cancer risk from snus is extremely low, long term use may result in highly undesirable keratotic lesions and associated epithelial abnormalities in the oral cavity. The rat forestomach, which is vulnerable to the irritative action of non-genotoxic compounds like butylated hydroxyanisole, propionic acid as well as snus, was chosen as an experimental model. Studied toxicological endpoints included histopathology and cellular proliferation based on DNA incorporation of bromodeoxyuridine. After 6 weeks exposure, blueberries (bilberries) and an extract from the common milk thistle were found to exert a highly significant inhibition of cell proliferation induced by snus in the rat forestomach epithelium, indicating a potential protection with respect soft tissue changes in the human oral cavity. (C) 2016 Elsevier Inc. All rights reserved.
PB  - Elsevier
T2  - Regulatory Toxicology and Pharmacology
T1  - Inhibition by blueberries (bilberries) and extract from milk thistle of rat forestomach hyperplasia induced by oral smokeless tobacco (Swedish snus)
VL  - 76
SP  - 94
EP  - 101
DO  - 10.1016/j.yrtph.2016.01.017
ER  - 

@article{
author = "Nilsson, Robert and Mićić, Mileva and Filipović, Jelena G. and Valenta-Šobot, Ana and Drakulić, Dunja R. and Stanojlović, Miloš R. and Joksić, Gordana",
year = "2016",
abstract = "The aim of this study was to identify palatable additives which have a significant protective action against soft tissue changes in the oral cavity caused by Swedish smokeless tobacco (snus), and that satisfy existing legal requirements. Although the cancer risk from snus is extremely low, long term use may result in highly undesirable keratotic lesions and associated epithelial abnormalities in the oral cavity. The rat forestomach, which is vulnerable to the irritative action of non-genotoxic compounds like butylated hydroxyanisole, propionic acid as well as snus, was chosen as an experimental model. Studied toxicological endpoints included histopathology and cellular proliferation based on DNA incorporation of bromodeoxyuridine. After 6 weeks exposure, blueberries (bilberries) and an extract from the common milk thistle were found to exert a highly significant inhibition of cell proliferation induced by snus in the rat forestomach epithelium, indicating a potential protection with respect soft tissue changes in the human oral cavity. (C) 2016 Elsevier Inc. All rights reserved.",
publisher = "Elsevier",
journal = "Regulatory Toxicology and Pharmacology",
title = "Inhibition by blueberries (bilberries) and extract from milk thistle of rat forestomach hyperplasia induced by oral smokeless tobacco (Swedish snus)",
volume = "76",
pages = "94-101",
doi = "10.1016/j.yrtph.2016.01.017"
}

Nilsson, R., Mićić, M., Filipović, J. G., Valenta-Šobot, A., Drakulić, D. R., Stanojlović, M. R.,& Joksić, G.. (2016). Inhibition by blueberries (bilberries) and extract from milk thistle of rat forestomach hyperplasia induced by oral smokeless tobacco (Swedish snus). in Regulatory Toxicology and Pharmacology
Elsevier., 76, 94-101.
https://doi.org/10.1016/j.yrtph.2016.01.017

Nilsson R, Mićić M, Filipović JG, Valenta-Šobot A, Drakulić DR, Stanojlović MR, Joksić G. Inhibition by blueberries (bilberries) and extract from milk thistle of rat forestomach hyperplasia induced by oral smokeless tobacco (Swedish snus). in Regulatory Toxicology and Pharmacology. 2016;76:94-101.
doi:10.1016/j.yrtph.2016.01.017 .

Nilsson, Robert, Mićić, Mileva, Filipović, Jelena G., Valenta-Šobot, Ana, Drakulić, Dunja R., Stanojlović, Miloš R., Joksić, Gordana, "Inhibition by blueberries (bilberries) and extract from milk thistle of rat forestomach hyperplasia induced by oral smokeless tobacco (Swedish snus)" in Regulatory Toxicology and Pharmacology, 76 (2016):94-101,
https://doi.org/10.1016/j.yrtph.2016.01.017 . .

TY  - JOUR
AU  - Nilsson, Robert
AU  - Antic, Ruza
AU  - Berni, Andrea
AU  - Dallner, Gustav
AU  - Dettbarn, Gerhard
AU  - Gromadzinska, Jolanta
AU  - Joksić, Gordana
AU  - Lundin, Cecilia
AU  - Palitti, Fabrizio
AU  - Prochazka, Gabriela
AU  - Rydzynski, Konrad
AU  - Segerback, Dan
AU  - Soucek, Pavel
AU  - Tekle, Michael
AU  - Seidel, Albrecht
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5310
AB  - Exposure of the general population to polycyclic aromatic hydrocarbons (PAH) is ubiquitous. The aim of this study was to analyze biomarkers associated with the uptake of PAH in 428 non-smoking women from Lodz (Poland), Viterbo (Italy), Belgrade (Serbia) and from the Pancevo area, where the petrochemical complex was destroyed by the air raids in 1999. Urinary excretion of PAH metabolites was lowest in Italian women, intermediary for Serbian and highest in Polish women, who predominantly excreted hydroxy phenanthrenes as metabolites of phenanthrene. Bulky DNA adduct levels were highest in Italian and Polish women. Genotype or PAH ambient air levels could not explain the dissimilarities between the study groups with respect to biomarker patterns, which probably reflected differences in life style-associated factors.
T2  - Biomarkers
T1  - Exposure to polycyclic aromatic hydrocarbons in women from Poland, Serbia and Italy - relation between PAH metabolite excretion, DNA damage, diet and genotype (the EU DIEPHY project)
VL  - 18
IS  - 2
SP  - 165
EP  - 173
DO  - 10.3109/1354750X.2012.762807
ER  - 

@article{
author = "Nilsson, Robert and Antic, Ruza and Berni, Andrea and Dallner, Gustav and Dettbarn, Gerhard and Gromadzinska, Jolanta and Joksić, Gordana and Lundin, Cecilia and Palitti, Fabrizio and Prochazka, Gabriela and Rydzynski, Konrad and Segerback, Dan and Soucek, Pavel and Tekle, Michael and Seidel, Albrecht",
year = "2013",
abstract = "Exposure of the general population to polycyclic aromatic hydrocarbons (PAH) is ubiquitous. The aim of this study was to analyze biomarkers associated with the uptake of PAH in 428 non-smoking women from Lodz (Poland), Viterbo (Italy), Belgrade (Serbia) and from the Pancevo area, where the petrochemical complex was destroyed by the air raids in 1999. Urinary excretion of PAH metabolites was lowest in Italian women, intermediary for Serbian and highest in Polish women, who predominantly excreted hydroxy phenanthrenes as metabolites of phenanthrene. Bulky DNA adduct levels were highest in Italian and Polish women. Genotype or PAH ambient air levels could not explain the dissimilarities between the study groups with respect to biomarker patterns, which probably reflected differences in life style-associated factors.",
journal = "Biomarkers",
title = "Exposure to polycyclic aromatic hydrocarbons in women from Poland, Serbia and Italy - relation between PAH metabolite excretion, DNA damage, diet and genotype (the EU DIEPHY project)",
volume = "18",
number = "2",
pages = "165-173",
doi = "10.3109/1354750X.2012.762807"
}

Nilsson, R., Antic, R., Berni, A., Dallner, G., Dettbarn, G., Gromadzinska, J., Joksić, G., Lundin, C., Palitti, F., Prochazka, G., Rydzynski, K., Segerback, D., Soucek, P., Tekle, M.,& Seidel, A.. (2013). Exposure to polycyclic aromatic hydrocarbons in women from Poland, Serbia and Italy - relation between PAH metabolite excretion, DNA damage, diet and genotype (the EU DIEPHY project). in Biomarkers, 18(2), 165-173.
https://doi.org/10.3109/1354750X.2012.762807

Nilsson R, Antic R, Berni A, Dallner G, Dettbarn G, Gromadzinska J, Joksić G, Lundin C, Palitti F, Prochazka G, Rydzynski K, Segerback D, Soucek P, Tekle M, Seidel A. Exposure to polycyclic aromatic hydrocarbons in women from Poland, Serbia and Italy - relation between PAH metabolite excretion, DNA damage, diet and genotype (the EU DIEPHY project). in Biomarkers. 2013;18(2):165-173.
doi:10.3109/1354750X.2012.762807 .

Nilsson, Robert, Antic, Ruza, Berni, Andrea, Dallner, Gustav, Dettbarn, Gerhard, Gromadzinska, Jolanta, Joksić, Gordana, Lundin, Cecilia, Palitti, Fabrizio, Prochazka, Gabriela, Rydzynski, Konrad, Segerback, Dan, Soucek, Pavel, Tekle, Michael, Seidel, Albrecht, "Exposure to polycyclic aromatic hydrocarbons in women from Poland, Serbia and Italy - relation between PAH metabolite excretion, DNA damage, diet and genotype (the EU DIEPHY project)" in Biomarkers, 18, no. 2 (2013):165-173,
https://doi.org/10.3109/1354750X.2012.762807 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Spasojević-Tišma, Vera D.
AU  - Antic, Ruza
AU  - Nilsson, Robert
AU  - Rutqvist, Lars Erik
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4346
AB  - Background: Epidemiological studies suggest that smokeless tobacco in the form of Swedish snus has been used by many smokers in Scandinavia to quit smoking, but the efficacy of snus has so far not been evaluated in controlled clinical trials. Methods: We conducted a randomized, double-blind, placebo-controlled, clinical trial aimed at assessing the efficacy of snus to help adult cigarette smokers in Serbia to substantially reduce, and, eventually, completely stop smoking. The study enrolled 319 healthy smokers aged 20-65 years at two occupational health centers in Belgrade, Serbia. Most of them (81%) expressed an interest to quit rather than just reduce their smoking. Study products were used ad libitum throughout the 48-week study period. The main study objective during the first 24 weeks was smoking reduction. The primary end-point was defined as a biologically verified reduction of GT = 50% in the average number of smoked cigarettes per day during week 21-24 compared to baseline. During week 25-48 participants were actively instructed to stop smoking completely. Outcome measures of biologically verified, complete smoking cessation included 1-week point prevalence rates at clinical visits after 12, 24, 36, and 48 weeks, as well as 4-, 12- and 24-week continued cessation rates at the week 36 and 48 visits. Results: At the week 24 visit, the proportion of participants who achieved the protocol definition of a GT = 50% smoking reduction was similar in the two treatment groups. However, the proportion that reported more extreme reductions ( GT = 75%) was statistically significantly higher in the snus group than in the placebo group (p LT 0.01). The results for biologically verified complete cessation suggested that participants in the snus group were more likely to quit smoking completely than the controls; the odds ratio (snus versus placebo) for the protocol estimates of cessation varied between 1.9 to 3.4, but these ratios were of borderline significance with p-values ranging from 0.04-0.10. Snus was well tolerated and only 2/158 (1.3%) participants in the snus group discontinued treatment due to an adverse event (in both cases unrelated to snus). Conclusions: Swedish snus could promote smoking cessation among smokers in Serbia, that is, in a cultural setting without traditional use of oral, smokeless tobacco.
T2  - Harm Reduction Journal
T1  - Randomized, placebo-controlled, double-blind trial of Swedish snus for smoking reduction and cessation
VL  - 8
DO  - 10.1186/1477-7517-8-25
ER  - 

@article{
author = "Joksić, Gordana and Spasojević-Tišma, Vera D. and Antic, Ruza and Nilsson, Robert and Rutqvist, Lars Erik",
year = "2011",
abstract = "Background: Epidemiological studies suggest that smokeless tobacco in the form of Swedish snus has been used by many smokers in Scandinavia to quit smoking, but the efficacy of snus has so far not been evaluated in controlled clinical trials. Methods: We conducted a randomized, double-blind, placebo-controlled, clinical trial aimed at assessing the efficacy of snus to help adult cigarette smokers in Serbia to substantially reduce, and, eventually, completely stop smoking. The study enrolled 319 healthy smokers aged 20-65 years at two occupational health centers in Belgrade, Serbia. Most of them (81%) expressed an interest to quit rather than just reduce their smoking. Study products were used ad libitum throughout the 48-week study period. The main study objective during the first 24 weeks was smoking reduction. The primary end-point was defined as a biologically verified reduction of GT = 50% in the average number of smoked cigarettes per day during week 21-24 compared to baseline. During week 25-48 participants were actively instructed to stop smoking completely. Outcome measures of biologically verified, complete smoking cessation included 1-week point prevalence rates at clinical visits after 12, 24, 36, and 48 weeks, as well as 4-, 12- and 24-week continued cessation rates at the week 36 and 48 visits. Results: At the week 24 visit, the proportion of participants who achieved the protocol definition of a GT = 50% smoking reduction was similar in the two treatment groups. However, the proportion that reported more extreme reductions ( GT = 75%) was statistically significantly higher in the snus group than in the placebo group (p LT 0.01). The results for biologically verified complete cessation suggested that participants in the snus group were more likely to quit smoking completely than the controls; the odds ratio (snus versus placebo) for the protocol estimates of cessation varied between 1.9 to 3.4, but these ratios were of borderline significance with p-values ranging from 0.04-0.10. Snus was well tolerated and only 2/158 (1.3%) participants in the snus group discontinued treatment due to an adverse event (in both cases unrelated to snus). Conclusions: Swedish snus could promote smoking cessation among smokers in Serbia, that is, in a cultural setting without traditional use of oral, smokeless tobacco.",
journal = "Harm Reduction Journal",
title = "Randomized, placebo-controlled, double-blind trial of Swedish snus for smoking reduction and cessation",
volume = "8",
doi = "10.1186/1477-7517-8-25"
}

Joksić, G., Spasojević-Tišma, V. D., Antic, R., Nilsson, R.,& Rutqvist, L. E.. (2011). Randomized, placebo-controlled, double-blind trial of Swedish snus for smoking reduction and cessation. in Harm Reduction Journal, 8.
https://doi.org/10.1186/1477-7517-8-25

Joksić G, Spasojević-Tišma VD, Antic R, Nilsson R, Rutqvist LE. Randomized, placebo-controlled, double-blind trial of Swedish snus for smoking reduction and cessation. in Harm Reduction Journal. 2011;8.
doi:10.1186/1477-7517-8-25 .

Joksić, Gordana, Spasojević-Tišma, Vera D., Antic, Ruza, Nilsson, Robert, Rutqvist, Lars Erik, "Randomized, placebo-controlled, double-blind trial of Swedish snus for smoking reduction and cessation" in Harm Reduction Journal, 8 (2011),
https://doi.org/10.1186/1477-7517-8-25 . .