TY  - JOUR
AU  - Abu el Rub, Anamarija
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Radoičić, Marija
AU  - Novaković, Mirjana
AU  - Žakula, Jelena
AU  - Ralić, Vanja
AU  - Petković, Marijana
AU  - Stepić, Milutin
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13214
AB  - The increasing incidence rate of pancreatic cancer worldwide, along with high resistance to existing chemotherapies and radiotherapy, demand new approaches for its treatment. Nanoparticle-guided cancer treatment modalities are promising in their anti-cancer efficiency and upgrading potential. We have synthesized and characterized Gd-doped titanium-dioxide nanoparticles (Gd-TiO 2 NPs), which showed good biocompatibility with MIA PaCa-2 and PANC-1 pancreatic cancer cell lines. To assess the effect that these NPs have on tumor cells, we used synchrotron radiation Fourier transform infrared spectroscopy and principal component analysis. We elucidated the biochemical changes in the two cell lines induced after their interaction with these NPs. Results demonstrated changes in nucleic acids and carbohydrate metabolism and alterations in the protein conformation in both cell lines. In addition, considerable changes in lipid area were observed in PANC-1 cell line, suggesting that Gd-TiO 2 NPs have a prospective to be further studied as systems for pancreatic cancer treatment.
T2  - Journal of Molecular Structure
T1  - Synchrotron radiation-FTIR biomolecular assessment in PANC-1 and MIA PaCa-2 pancreatic cancer cells after treatment with Gd-TiO2 nanoparticles
VL  - 1309
SP  - 138167
DO  - 10.1016/j.molstruc.2024.138167
ER  - 

@article{
author = "Abu el Rub, Anamarija and Nešić, Maja D. and Dučić, Tanja and Radoičić, Marija and Novaković, Mirjana and Žakula, Jelena and Ralić, Vanja and Petković, Marijana and Stepić, Milutin",
year = "2024",
abstract = "The increasing incidence rate of pancreatic cancer worldwide, along with high resistance to existing chemotherapies and radiotherapy, demand new approaches for its treatment. Nanoparticle-guided cancer treatment modalities are promising in their anti-cancer efficiency and upgrading potential. We have synthesized and characterized Gd-doped titanium-dioxide nanoparticles (Gd-TiO 2 NPs), which showed good biocompatibility with MIA PaCa-2 and PANC-1 pancreatic cancer cell lines. To assess the effect that these NPs have on tumor cells, we used synchrotron radiation Fourier transform infrared spectroscopy and principal component analysis. We elucidated the biochemical changes in the two cell lines induced after their interaction with these NPs. Results demonstrated changes in nucleic acids and carbohydrate metabolism and alterations in the protein conformation in both cell lines. In addition, considerable changes in lipid area were observed in PANC-1 cell line, suggesting that Gd-TiO 2 NPs have a prospective to be further studied as systems for pancreatic cancer treatment.",
journal = "Journal of Molecular Structure",
title = "Synchrotron radiation-FTIR biomolecular assessment in PANC-1 and MIA PaCa-2 pancreatic cancer cells after treatment with Gd-TiO2 nanoparticles",
volume = "1309",
pages = "138167",
doi = "10.1016/j.molstruc.2024.138167"
}

Abu el Rub, A., Nešić, M. D., Dučić, T., Radoičić, M., Novaković, M., Žakula, J., Ralić, V., Petković, M.,& Stepić, M.. (2024). Synchrotron radiation-FTIR biomolecular assessment in PANC-1 and MIA PaCa-2 pancreatic cancer cells after treatment with Gd-TiO2 nanoparticles. in Journal of Molecular Structure, 1309, 138167.
https://doi.org/10.1016/j.molstruc.2024.138167

Abu el Rub A, Nešić MD, Dučić T, Radoičić M, Novaković M, Žakula J, Ralić V, Petković M, Stepić M. Synchrotron radiation-FTIR biomolecular assessment in PANC-1 and MIA PaCa-2 pancreatic cancer cells after treatment with Gd-TiO2 nanoparticles. in Journal of Molecular Structure. 2024;1309:138167.
doi:10.1016/j.molstruc.2024.138167 .

Abu el Rub, Anamarija, Nešić, Maja D., Dučić, Tanja, Radoičić, Marija, Novaković, Mirjana, Žakula, Jelena, Ralić, Vanja, Petković, Marijana, Stepić, Milutin, "Synchrotron radiation-FTIR biomolecular assessment in PANC-1 and MIA PaCa-2 pancreatic cancer cells after treatment with Gd-TiO2 nanoparticles" in Journal of Molecular Structure, 1309 (2024):138167,
https://doi.org/10.1016/j.molstruc.2024.138167 . .

TY  - CONF
AU  - Nešić, Maja D.
AU  - Popović, Iva
AU  - Ralić, Vanja
AU  - Dučić, Tanja
AU  - Algarra, M.
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13564
PB  - Portugal : SPOF - Sociedade Portuguesa para a Investigação e Desenvolvimento em Óptica e Fotónica
C3  - AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts
T1  - Synchrotron Radiation Fourier Transform Infrared spectroscopical analysis of biomolecules in MRC-5 fibroblast cells treated with biomass-derived carbon dots
SP  - 49
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13564
ER  - 

@conference{
author = "Nešić, Maja D. and Popović, Iva and Ralić, Vanja and Dučić, Tanja and Algarra, M. and Stepić, Milutin and Petković, Marijana",
year = "2024",
publisher = "Portugal : SPOF - Sociedade Portuguesa para a Investigação e Desenvolvimento em Óptica e Fotónica",
journal = "AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts",
title = "Synchrotron Radiation Fourier Transform Infrared spectroscopical analysis of biomolecules in MRC-5 fibroblast cells treated with biomass-derived carbon dots",
pages = "49-49",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13564"
}

Nešić, M. D., Popović, I., Ralić, V., Dučić, T., Algarra, M., Stepić, M.,& Petković, M.. (2024). Synchrotron Radiation Fourier Transform Infrared spectroscopical analysis of biomolecules in MRC-5 fibroblast cells treated with biomass-derived carbon dots. in AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts
Portugal : SPOF - Sociedade Portuguesa para a Investigação e Desenvolvimento em Óptica e Fotónica., 49-49.
https://hdl.handle.net/21.15107/rcub_vinar_13564

Nešić MD, Popović I, Ralić V, Dučić T, Algarra M, Stepić M, Petković M. Synchrotron Radiation Fourier Transform Infrared spectroscopical analysis of biomolecules in MRC-5 fibroblast cells treated with biomass-derived carbon dots. in AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts. 2024;:49-49.
https://hdl.handle.net/21.15107/rcub_vinar_13564 .

Nešić, Maja D., Popović, Iva, Ralić, Vanja, Dučić, Tanja, Algarra, M., Stepić, Milutin, Petković, Marijana, "Synchrotron Radiation Fourier Transform Infrared spectroscopical analysis of biomolecules in MRC-5 fibroblast cells treated with biomass-derived carbon dots" in AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts (2024):49-49,
https://hdl.handle.net/21.15107/rcub_vinar_13564 .

TY  - CONF
AU  - Popović, Iva
AU  - Nišavić, Marija
AU  - Ralić, Vanja
AU  - Petković, Marijana
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Stepić, Milutin
AU  - Nešić, Maja D.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13567
AB  - Chemotherapy is one of the most effective approaches used to treat cancer, a leading
cause of death worldwide.[1] Ruthenium-based complexes have emerged as a powerful
alternative to state-of-the-art platinum chemotherapeutics due to their reduced toxicity,
higher selectivity and anti-metastatic potential.[2] Here, we employ mass spectrometry and
spectroscopic techniques to characterize a novel cis-[Ru(II)(dcbpy)2Cl2] complex and
investigate its interaction with cellular lipids. In addition, the cytotoxic effect and mode of
action on four standard cancer cell lines, namely melanoma A375, cervical cancer HeLa and
pancreas ductal adenocarcinoma MIA PaCa-2 and PANC-1, were elucidated. Our results
show a dose-dependent cytotoxicity of the complex on A375, MIA PaCa-2 and PANC-1.
Further, the Ru(II) complex reduces the clonogenic potential caused by G1 arrest and
increases ROS production in all tested cell lines. Lipid peroxidation is also observed in
HeLa and MIA PaCa-2 cells, possibly indicating ferroptosis induction. Synchrotron
radiation Fourier Transform Infrared Spectroscopy was applied to study the overall
interaction of a Ru-complex with cellular biomolecules, and our results demonstrated a shift
in bands in the lipid fingerprint area of most cell lines, confirming the lipid rearrangement
process induced by the interaction with Ru(II) complex. The complex significantly
increases the percentage of apoptotic A375, HeLa and MIA PaCa-2, while a significant
decrease of p62 protein, a marker for autophagy induction, is detected solely in A375 cells.
The wound healing assay revealed that the Ru(II) complex reduces the metastatic potential
of HeLa and MIA PaCa-2 cells by reducing their migration ability. Overall, our results
indicate the existence of a general mode of action of the tested Ru(II) complex that involves
growth arrest and ROS production, as well as cancer cell line-specific effects. These involve
dual role trough inducing programmed cell death - apoptosis and autophagy as seen in A375
cells, or apoptosis and ferroptosis as indicated by the results obtained on HeLa and MIA
PaCa-2 cells, where anti-metastatic potential was also observed. This work highlights the
multimodal action of Ru(II) complexes and the broadness of their application as potential
chemotherapeutic agents for treating different cancers.[3]
C3  - ICNI-III : 3rd International Conference on Noncovalent Interactions : Book of abstracts
T1  - The interaction of a novel Ru(II) complex with cellular lipids and induction of programed cell death in cancer cell lines
SP  - P12
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13567
ER  - 

@conference{
author = "Popović, Iva and Nišavić, Marija and Ralić, Vanja and Petković, Marijana and Žakula, Jelena and Korićanac, Lela and Stepić, Milutin and Nešić, Maja D.",
year = "2024",
abstract = "Chemotherapy is one of the most effective approaches used to treat cancer, a leading
cause of death worldwide.[1] Ruthenium-based complexes have emerged as a powerful
alternative to state-of-the-art platinum chemotherapeutics due to their reduced toxicity,
higher selectivity and anti-metastatic potential.[2] Here, we employ mass spectrometry and
spectroscopic techniques to characterize a novel cis-[Ru(II)(dcbpy)2Cl2] complex and
investigate its interaction with cellular lipids. In addition, the cytotoxic effect and mode of
action on four standard cancer cell lines, namely melanoma A375, cervical cancer HeLa and
pancreas ductal adenocarcinoma MIA PaCa-2 and PANC-1, were elucidated. Our results
show a dose-dependent cytotoxicity of the complex on A375, MIA PaCa-2 and PANC-1.
Further, the Ru(II) complex reduces the clonogenic potential caused by G1 arrest and
increases ROS production in all tested cell lines. Lipid peroxidation is also observed in
HeLa and MIA PaCa-2 cells, possibly indicating ferroptosis induction. Synchrotron
radiation Fourier Transform Infrared Spectroscopy was applied to study the overall
interaction of a Ru-complex with cellular biomolecules, and our results demonstrated a shift
in bands in the lipid fingerprint area of most cell lines, confirming the lipid rearrangement
process induced by the interaction with Ru(II) complex. The complex significantly
increases the percentage of apoptotic A375, HeLa and MIA PaCa-2, while a significant
decrease of p62 protein, a marker for autophagy induction, is detected solely in A375 cells.
The wound healing assay revealed that the Ru(II) complex reduces the metastatic potential
of HeLa and MIA PaCa-2 cells by reducing their migration ability. Overall, our results
indicate the existence of a general mode of action of the tested Ru(II) complex that involves
growth arrest and ROS production, as well as cancer cell line-specific effects. These involve
dual role trough inducing programmed cell death - apoptosis and autophagy as seen in A375
cells, or apoptosis and ferroptosis as indicated by the results obtained on HeLa and MIA
PaCa-2 cells, where anti-metastatic potential was also observed. This work highlights the
multimodal action of Ru(II) complexes and the broadness of their application as potential
chemotherapeutic agents for treating different cancers.[3]",
journal = "ICNI-III : 3rd International Conference on Noncovalent Interactions : Book of abstracts",
title = "The interaction of a novel Ru(II) complex with cellular lipids and induction of programed cell death in cancer cell lines",
pages = "P12",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13567"
}

Popović, I., Nišavić, M., Ralić, V., Petković, M., Žakula, J., Korićanac, L., Stepić, M.,& Nešić, M. D.. (2024). The interaction of a novel Ru(II) complex with cellular lipids and induction of programed cell death in cancer cell lines. in ICNI-III : 3rd International Conference on Noncovalent Interactions : Book of abstracts, P12.
https://hdl.handle.net/21.15107/rcub_vinar_13567

Popović I, Nišavić M, Ralić V, Petković M, Žakula J, Korićanac L, Stepić M, Nešić MD. The interaction of a novel Ru(II) complex with cellular lipids and induction of programed cell death in cancer cell lines. in ICNI-III : 3rd International Conference on Noncovalent Interactions : Book of abstracts. 2024;:P12.
https://hdl.handle.net/21.15107/rcub_vinar_13567 .

Popović, Iva, Nišavić, Marija, Ralić, Vanja, Petković, Marijana, Žakula, Jelena, Korićanac, Lela, Stepić, Milutin, Nešić, Maja D., "The interaction of a novel Ru(II) complex with cellular lipids and induction of programed cell death in cancer cell lines" in ICNI-III : 3rd International Conference on Noncovalent Interactions : Book of abstracts (2024):P12,
https://hdl.handle.net/21.15107/rcub_vinar_13567 .

TY  - CONF
AU  - Popović, Iva
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Ralić, Vanja
AU  - Abu el Rub, Anamarija
AU  - Algarra, Manuel
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Nešić, Maja D.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11808
AB  - In recent years researchers have developed new strategies to enhance the effectiveness of wound healing by combining nanoparticles and infra red (IR) light. For example, some studies have shown that nanoparticles can be used to enhance the absorption of near-infrared laser (NIR) light by tissues, leading to increased healing rates [1]. The influence of NIR light on proliferation, collagen production, and wound healing was tested on: keratocytes (HaCaT) and fibroblasts (MRC-5) cells that are used as model systems of human skin equivalents that comprise an epidermal and a dermal compartment of skin. Also, these cells were treated with carbon quantum dots/silver-based metal-organic framework composites (Ag-MoFs-NCDs and Ag-MoFs-SCDs), which previously showed high antibacterial activity [2], without and with laser light. Firstly, we have found the most convenient and effective CW laser intensity (16 mW/cm2) and illumination time (3 minutes), which is not too high and short enough to influence human cells' proliferation and metabolism positively. Additional chemical treatment with Ag-MoFs-NCDs and Ag-MoFs-SCDs results in a further increase in human cell viability. Our measurements showed that the proliferation index in laser-illuminated cells and cells treated with Ag-MoFs-SCDs was at the level of the untreated control. Furthermore, Ag-MoFs-SCDs treatment and laser illumination induced a mild, insignificant increase in cellular proliferation. On the other hand, Ag-MoFs-NCDs treatment led to a more pronounced, albeit not significant increase, in cellular proliferation, while Ag-MoFs-NCDs treatment combined with laser illumination significantly increased proliferation. Also, we have detected a mild change in collagen level estimated by hydroxyproline assay, which may indicate a positive outcome of combined laser illumination and treatment, taking into account that after 48 hours, a change in cell's response to the treatment could be noticed. Finally, based on migration assay, we observe a complete wound closure after 48 hours in fibroblast cells treated with Ag-MoFs-NCDs and near-infrared laser light, Fig. 1.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts
T1  - Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing
SP  - 78
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11808
ER  - 

@conference{
author = "Popović, Iva and Valenta Šobot, Ana and Filipović Tričković, Jelena and Korićanac, Lela and Žakula, Jelena and Ralić, Vanja and Abu el Rub, Anamarija and Algarra, Manuel and Petković, Marijana and Stepić, Milutin and Nešić, Maja D.",
year = "2023",
abstract = "In recent years researchers have developed new strategies to enhance the effectiveness of wound healing by combining nanoparticles and infra red (IR) light. For example, some studies have shown that nanoparticles can be used to enhance the absorption of near-infrared laser (NIR) light by tissues, leading to increased healing rates [1]. The influence of NIR light on proliferation, collagen production, and wound healing was tested on: keratocytes (HaCaT) and fibroblasts (MRC-5) cells that are used as model systems of human skin equivalents that comprise an epidermal and a dermal compartment of skin. Also, these cells were treated with carbon quantum dots/silver-based metal-organic framework composites (Ag-MoFs-NCDs and Ag-MoFs-SCDs), which previously showed high antibacterial activity [2], without and with laser light. Firstly, we have found the most convenient and effective CW laser intensity (16 mW/cm2) and illumination time (3 minutes), which is not too high and short enough to influence human cells' proliferation and metabolism positively. Additional chemical treatment with Ag-MoFs-NCDs and Ag-MoFs-SCDs results in a further increase in human cell viability. Our measurements showed that the proliferation index in laser-illuminated cells and cells treated with Ag-MoFs-SCDs was at the level of the untreated control. Furthermore, Ag-MoFs-SCDs treatment and laser illumination induced a mild, insignificant increase in cellular proliferation. On the other hand, Ag-MoFs-NCDs treatment led to a more pronounced, albeit not significant increase, in cellular proliferation, while Ag-MoFs-NCDs treatment combined with laser illumination significantly increased proliferation. Also, we have detected a mild change in collagen level estimated by hydroxyproline assay, which may indicate a positive outcome of combined laser illumination and treatment, taking into account that after 48 hours, a change in cell's response to the treatment could be noticed. Finally, based on migration assay, we observe a complete wound closure after 48 hours in fibroblast cells treated with Ag-MoFs-NCDs and near-infrared laser light, Fig. 1.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts",
title = "Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing",
pages = "78-78",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11808"
}

Popović, I., Valenta Šobot, A., Filipović Tričković, J., Korićanac, L., Žakula, J., Ralić, V., Abu el Rub, A., Algarra, M., Petković, M., Stepić, M.,& Nešić, M. D.. (2023). Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing. in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts
Belgrade : Vinča Institute of Nuclear Sciences., 78-78.
https://hdl.handle.net/21.15107/rcub_vinar_11808

Popović I, Valenta Šobot A, Filipović Tričković J, Korićanac L, Žakula J, Ralić V, Abu el Rub A, Algarra M, Petković M, Stepić M, Nešić MD. Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing. in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts. 2023;:78-78.
https://hdl.handle.net/21.15107/rcub_vinar_11808 .

Popović, Iva, Valenta Šobot, Ana, Filipović Tričković, Jelena, Korićanac, Lela, Žakula, Jelena, Ralić, Vanja, Abu el Rub, Anamarija, Algarra, Manuel, Petković, Marijana, Stepić, Milutin, Nešić, Maja D., "Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing" in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts (2023):78-78,
https://hdl.handle.net/21.15107/rcub_vinar_11808 .

TY  - CONF
AU  - Ralić, Vanja
AU  - Gemović, Branislava
AU  - Nešić, Maja D.
AU  - Popović, Iva
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12390
AB  - In this work, we have applied the Informational Spectrum Method (ISM) to discover a potential protein target in the HeLa cervical cancer cell line for [Pd(dach)Cl2] complex. Since Pd complexes are considered an alternative to traditionally used Pt complexes in anti-cancer therapy, it is essential to elucidate the mechanism of their action. A detailed analysis that also involves screening the known protein databases revealed the proteins of the SOSS complex as the most probable [Pd(dach)Cl2] targets. Since this protein maintains genomic stability, this result shows the potential of the Pd(II) complex as an anti-cancer drug.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells
SP  - 296
EP  - 299
DO  - 10.46793/ICCBI23.296R
ER  - 

@conference{
author = "Ralić, Vanja and Gemović, Branislava and Nešić, Maja D. and Popović, Iva and Stepić, Milutin and Petković, Marijana",
year = "2023",
abstract = "In this work, we have applied the Informational Spectrum Method (ISM) to discover a potential protein target in the HeLa cervical cancer cell line for [Pd(dach)Cl2] complex. Since Pd complexes are considered an alternative to traditionally used Pt complexes in anti-cancer therapy, it is essential to elucidate the mechanism of their action. A detailed analysis that also involves screening the known protein databases revealed the proteins of the SOSS complex as the most probable [Pd(dach)Cl2] targets. Since this protein maintains genomic stability, this result shows the potential of the Pd(II) complex as an anti-cancer drug.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells",
pages = "296-299",
doi = "10.46793/ICCBI23.296R"
}

Ralić, V., Gemović, B., Nešić, M. D., Popović, I., Stepić, M.,& Petković, M.. (2023). Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 296-299.
https://doi.org/10.46793/ICCBI23.296R

Ralić V, Gemović B, Nešić MD, Popović I, Stepić M, Petković M. Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:296-299.
doi:10.46793/ICCBI23.296R .

Ralić, Vanja, Gemović, Branislava, Nešić, Maja D., Popović, Iva, Stepić, Milutin, Petković, Marijana, "Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):296-299,
https://doi.org/10.46793/ICCBI23.296R . .

TY  - CONF
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Popović, Iva
AU  - Algarra, Manuel
AU  - Dučić, Tanja
AU  - Abu el Rub, Anamarija
AU  - Ralić, Vanja
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Nešić, Maja D.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13568
AB  - Small, carbon-based nanoparticles, carbon dots (CDs), are investigated as photosensitizers (PSs) for photodynamic therapy of cancers. The results presented here show biochemical changes in cancer cells treated with surface-modified CDs, which are augmented by illumination, indicating a high potential of CDs as selective drug carriers and potent PSs. In addition, we will show that CDs produced from black carrot extract (biomass) can be exploited for image-guided therapy and be cytotoxic for cancer cells, thanks to their fluorescence properties. Finally, since biomass-fabricated CDs interact strongly with nucleic acids, they can be further developed for cell cycle sensing purposes.
PB  - Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - Application of Carbon-Based Nanocomposite Systems as Photosensitizers for Photodynamic Therapy
SP  - 61
EP  - 66
DO  - 10.46793/ICCBI23.061P
ER  - 

@conference{
author = "Petković, Marijana and Stepić, Milutin and Popović, Iva and Algarra, Manuel and Dučić, Tanja and Abu el Rub, Anamarija and Ralić, Vanja and Korićanac, Lela and Žakula, Jelena and Filipović Tričković, Jelena and Valenta Šobot, Ana and Nešić, Maja D.",
year = "2023",
abstract = "Small, carbon-based nanoparticles, carbon dots (CDs), are investigated as photosensitizers (PSs) for photodynamic therapy of cancers. The results presented here show biochemical changes in cancer cells treated with surface-modified CDs, which are augmented by illumination, indicating a high potential of CDs as selective drug carriers and potent PSs. In addition, we will show that CDs produced from black carrot extract (biomass) can be exploited for image-guided therapy and be cytotoxic for cancer cells, thanks to their fluorescence properties. Finally, since biomass-fabricated CDs interact strongly with nucleic acids, they can be further developed for cell cycle sensing purposes.",
publisher = "Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "Application of Carbon-Based Nanocomposite Systems as Photosensitizers for Photodynamic Therapy",
pages = "61-66",
doi = "10.46793/ICCBI23.061P"
}

Petković, M., Stepić, M., Popović, I., Algarra, M., Dučić, T., Abu el Rub, A., Ralić, V., Korićanac, L., Žakula, J., Filipović Tričković, J., Valenta Šobot, A.,& Nešić, M. D.. (2023). Application of Carbon-Based Nanocomposite Systems as Photosensitizers for Photodynamic Therapy. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Institute for Information Technologies, University of Kragujevac., 61-66.
https://doi.org/10.46793/ICCBI23.061P

Petković M, Stepić M, Popović I, Algarra M, Dučić T, Abu el Rub A, Ralić V, Korićanac L, Žakula J, Filipović Tričković J, Valenta Šobot A, Nešić MD. Application of Carbon-Based Nanocomposite Systems as Photosensitizers for Photodynamic Therapy. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:61-66.
doi:10.46793/ICCBI23.061P .

Petković, Marijana, Stepić, Milutin, Popović, Iva, Algarra, Manuel, Dučić, Tanja, Abu el Rub, Anamarija, Ralić, Vanja, Korićanac, Lela, Žakula, Jelena, Filipović Tričković, Jelena, Valenta Šobot, Ana, Nešić, Maja D., "Application of Carbon-Based Nanocomposite Systems as Photosensitizers for Photodynamic Therapy" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):61-66,
https://doi.org/10.46793/ICCBI23.061P . .

TY  - CONF
AU  - Nešić, M. D.
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Popović, Iva
AU  - Soto, J.
AU  - Algarra, Manuel
AU  - Ralić, Vanja
AU  - Abu el Rub, Anamarija
AU  - Matijević, Milica
AU  - Stepić, Milutin
AU  - Petković, M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11809
AB  - Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts
T1  - Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11809
ER  - 

@conference{
author = "Nešić, M. D. and Filipović Tričković, Jelena and Valenta Šobot, Ana and Žakula, Jelena and Korićanac, Lela and Popović, Iva and Soto, J. and Algarra, Manuel and Ralić, Vanja and Abu el Rub, Anamarija and Matijević, Milica and Stepić, Milutin and Petković, M.",
year = "2023",
abstract = "Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts",
title = "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11809"
}

Nešić, M. D., Filipović Tričković, J., Valenta Šobot, A., Žakula, J., Korićanac, L., Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, A., Matijević, M., Stepić, M.,& Petković, M.. (2023). Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts
Belgrade : Vinča Institute of Nuclear Sciences., 79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809

Nešić MD, Filipović Tričković J, Valenta Šobot A, Žakula J, Korićanac L, Popović I, Soto J, Algarra M, Ralić V, Abu el Rub A, Matijević M, Stepić M, Petković M. Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts. 2023;:79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

Nešić, M. D., Filipović Tričković, Jelena, Valenta Šobot, Ana, Žakula, Jelena, Korićanac, Lela, Popović, Iva, Soto, J., Algarra, Manuel, Ralić, Vanja, Abu el Rub, Anamarija, Matijević, Milica, Stepić, Milutin, Petković, M., "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles" in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts (2023):79-79,
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

TY  - CONF
AU  - Abu el Rub, Anamarija
AU  - Nešić, M. D.
AU  - Žakula, Jelena
AU  - Ralić, Vanja
AU  - Petković, M.
AU  - Popović, Iva
AU  - Matijević, Milica
AU  - Radoičić, Marija
AU  - Stepić, Milutin
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11810
AB  - With difficulty in early diagnosis, inaccessibility for the surgical approach, and high resistance to radio and chemotherapies, the resulting low treatment success rates are calling for new approaches in treating pancreatic cancer [1]. Photodynamic therapy (PDT), with the use of light or X-rays, is a method that has the potential to help overcome the problems that existing approaches meet [2,3]. Through activation of photo-sensitive particles with irradiation, PDT helps the production of reactive oxygen species, consequently stimulating cell death. We have synthesized and characterized Gadolinium-doped titanium dioxide nanoparticles (Gd-TiO2 NPs) and tested them as photosensitizers on two pancreatic cancer cell lines, MIA PaCa-2 and PANC-1. Different concentrations of NP treatment, irradiation powers, and times of irradiation were trialed as parameters of activation. Cell viability was measured 48h after treatments, and although some results implied a slight decrease in the viability of treated cells, we have met difficulties in obtaining consistency in results. Statistical significance in the decrease of the viability of treated cells in most cases was not attained, suggesting that higher concentrations or irradiation power and longer illumination time might be needed to achieve a positive PDT effect with this NP system.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts
T1  - In search of conditions for Gd-TiO2 activation by light irradiation in photodynamic treatment of pancreatic cancer cells
SP  - 80
EP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11810
ER  - 

@conference{
author = "Abu el Rub, Anamarija and Nešić, M. D. and Žakula, Jelena and Ralić, Vanja and Petković, M. and Popović, Iva and Matijević, Milica and Radoičić, Marija and Stepić, Milutin",
year = "2023",
abstract = "With difficulty in early diagnosis, inaccessibility for the surgical approach, and high resistance to radio and chemotherapies, the resulting low treatment success rates are calling for new approaches in treating pancreatic cancer [1]. Photodynamic therapy (PDT), with the use of light or X-rays, is a method that has the potential to help overcome the problems that existing approaches meet [2,3]. Through activation of photo-sensitive particles with irradiation, PDT helps the production of reactive oxygen species, consequently stimulating cell death. We have synthesized and characterized Gadolinium-doped titanium dioxide nanoparticles (Gd-TiO2 NPs) and tested them as photosensitizers on two pancreatic cancer cell lines, MIA PaCa-2 and PANC-1. Different concentrations of NP treatment, irradiation powers, and times of irradiation were trialed as parameters of activation. Cell viability was measured 48h after treatments, and although some results implied a slight decrease in the viability of treated cells, we have met difficulties in obtaining consistency in results. Statistical significance in the decrease of the viability of treated cells in most cases was not attained, suggesting that higher concentrations or irradiation power and longer illumination time might be needed to achieve a positive PDT effect with this NP system.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts",
title = "In search of conditions for Gd-TiO2 activation by light irradiation in photodynamic treatment of pancreatic cancer cells",
pages = "80-80",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11810"
}

Abu el Rub, A., Nešić, M. D., Žakula, J., Ralić, V., Petković, M., Popović, I., Matijević, M., Radoičić, M.,& Stepić, M.. (2023). In search of conditions for Gd-TiO2 activation by light irradiation in photodynamic treatment of pancreatic cancer cells. in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts
Belgrade : Vinča Institute of Nuclear Sciences., 80-80.
https://hdl.handle.net/21.15107/rcub_vinar_11810

Abu el Rub A, Nešić MD, Žakula J, Ralić V, Petković M, Popović I, Matijević M, Radoičić M, Stepić M. In search of conditions for Gd-TiO2 activation by light irradiation in photodynamic treatment of pancreatic cancer cells. in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts. 2023;:80-80.
https://hdl.handle.net/21.15107/rcub_vinar_11810 .

Abu el Rub, Anamarija, Nešić, M. D., Žakula, Jelena, Ralić, Vanja, Petković, M., Popović, Iva, Matijević, Milica, Radoičić, Marija, Stepić, Milutin, "In search of conditions for Gd-TiO2 activation by light irradiation in photodynamic treatment of pancreatic cancer cells" in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts (2023):80-80,
https://hdl.handle.net/21.15107/rcub_vinar_11810 .

TY  - CONF
AU  - Ralić, Vanja
AU  - Nešić, Maja
AU  - Abu el Rub, Anamarija
AU  - Dučić, Tanja
AU  - Gemović, Branislava
AU  - Algarra, Manuel
AU  - Stepić, Milutin
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petković, Marijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12657
AB  - Nanocomposite system formulated from surface-modified S-doped carbon dot (S-CD) nanoparticle with a potential metallodrug, palladium(II) complex, dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl2] (Pd@S-CD), was investigated as a model system for the treatment of cervical carcinoma (HeLa) cells. To examine the intracellular biochemical effects induced by the Pd@S-CD, we used Synchrotron Radiation-based Fourier-transform infrared spectroscopy (SR FTIR). SR FTIR spectroscopy was employed to investigate the alterations in cellular components’ biochemical composition and secondary structure upon exposure to Pd@S-CD. Spectral analysis, complemented by statistical techniques, revealed changes in biomolecules, lipids, proteins, nucleic acids, and carbohydrates caused by the treatment with Pd@CDs. These results and the increased cytotoxicity of the system demonstrate its high anti-cervical cancer therapeutic potential.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells
SP  - 467
EP  - 470
DO  - 10.46793/ICCBI23.467R
ER  - 

@conference{
author = "Ralić, Vanja and Nešić, Maja and Abu el Rub, Anamarija and Dučić, Tanja and Gemović, Branislava and Algarra, Manuel and Stepić, Milutin and Korićanac, Lela and Žakula, Jelena and Petković, Marijana",
year = "2023",
abstract = "Nanocomposite system formulated from surface-modified S-doped carbon dot (S-CD) nanoparticle with a potential metallodrug, palladium(II) complex, dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl2] (Pd@S-CD), was investigated as a model system for the treatment of cervical carcinoma (HeLa) cells. To examine the intracellular biochemical effects induced by the Pd@S-CD, we used Synchrotron Radiation-based Fourier-transform infrared spectroscopy (SR FTIR). SR FTIR spectroscopy was employed to investigate the alterations in cellular components’ biochemical composition and secondary structure upon exposure to Pd@S-CD. Spectral analysis, complemented by statistical techniques, revealed changes in biomolecules, lipids, proteins, nucleic acids, and carbohydrates caused by the treatment with Pd@CDs. These results and the increased cytotoxicity of the system demonstrate its high anti-cervical cancer therapeutic potential.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells",
pages = "467-470",
doi = "10.46793/ICCBI23.467R"
}

Ralić, V., Nešić, M., Abu el Rub, A., Dučić, T., Gemović, B., Algarra, M., Stepić, M., Korićanac, L., Žakula, J.,& Petković, M.. (2023). SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 467-470.
https://doi.org/10.46793/ICCBI23.467R

Ralić V, Nešić M, Abu el Rub A, Dučić T, Gemović B, Algarra M, Stepić M, Korićanac L, Žakula J, Petković M. SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:467-470.
doi:10.46793/ICCBI23.467R .

Ralić, Vanja, Nešić, Maja, Abu el Rub, Anamarija, Dučić, Tanja, Gemović, Branislava, Algarra, Manuel, Stepić, Milutin, Korićanac, Lela, Žakula, Jelena, Petković, Marijana, "SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):467-470,
https://doi.org/10.46793/ICCBI23.467R . .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Ralić, Vanja
AU  - Nastasijević, Branislav
AU  - Matijević, Milica
AU  - Vujčić, Zoran
AU  - Margetić, Aleksandra
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11988
AB  - Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone.
AB  - Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.
T2  - Journal of the Serbian Chemical Society
T1  - A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain
T1  - Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B
VL  - 88
IS  - 10
SP  - 985
EP  - 997
DO  - 10.2298/JSC221116011S
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Ralić, Vanja and Nastasijević, Branislav and Matijević, Milica and Vujčić, Zoran and Margetić, Aleksandra",
year = "2023",
abstract = "Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone., Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.",
journal = "Journal of the Serbian Chemical Society",
title = "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain, Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B",
volume = "88",
number = "10",
pages = "985-997",
doi = "10.2298/JSC221116011S"
}

Šokarda Slavić, M., Ralić, V., Nastasijević, B., Matijević, M., Vujčić, Z.,& Margetić, A.. (2023). A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society, 88(10), 985-997.
https://doi.org/10.2298/JSC221116011S

Šokarda Slavić M, Ralić V, Nastasijević B, Matijević M, Vujčić Z, Margetić A. A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society. 2023;88(10):985-997.
doi:10.2298/JSC221116011S .

Šokarda Slavić, Marinela, Ralić, Vanja, Nastasijević, Branislav, Matijević, Milica, Vujčić, Zoran, Margetić, Aleksandra, "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain" in Journal of the Serbian Chemical Society, 88, no. 10 (2023):985-997,
https://doi.org/10.2298/JSC221116011S . .