TY  - CONF
AU  - Ralić, Vanja
AU  - Gemović, Branislava
AU  - Nešić, Maja D.
AU  - Popović, Iva
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12390
AB  - In this work, we have applied the Informational Spectrum Method (ISM) to discover a potential protein target in the HeLa cervical cancer cell line for [Pd(dach)Cl2] complex. Since Pd complexes are considered an alternative to traditionally used Pt complexes in anti-cancer therapy, it is essential to elucidate the mechanism of their action. A detailed analysis that also involves screening the known protein databases revealed the proteins of the SOSS complex as the most probable [Pd(dach)Cl2] targets. Since this protein maintains genomic stability, this result shows the potential of the Pd(II) complex as an anti-cancer drug.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells
SP  - 296
EP  - 299
DO  - 10.46793/ICCBI23.296R
ER  - 

@conference{
author = "Ralić, Vanja and Gemović, Branislava and Nešić, Maja D. and Popović, Iva and Stepić, Milutin and Petković, Marijana",
year = "2023",
abstract = "In this work, we have applied the Informational Spectrum Method (ISM) to discover a potential protein target in the HeLa cervical cancer cell line for [Pd(dach)Cl2] complex. Since Pd complexes are considered an alternative to traditionally used Pt complexes in anti-cancer therapy, it is essential to elucidate the mechanism of their action. A detailed analysis that also involves screening the known protein databases revealed the proteins of the SOSS complex as the most probable [Pd(dach)Cl2] targets. Since this protein maintains genomic stability, this result shows the potential of the Pd(II) complex as an anti-cancer drug.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells",
pages = "296-299",
doi = "10.46793/ICCBI23.296R"
}

Ralić, V., Gemović, B., Nešić, M. D., Popović, I., Stepić, M.,& Petković, M.. (2023). Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 296-299.
https://doi.org/10.46793/ICCBI23.296R

Ralić V, Gemović B, Nešić MD, Popović I, Stepić M, Petković M. Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:296-299.
doi:10.46793/ICCBI23.296R .

Ralić, Vanja, Gemović, Branislava, Nešić, Maja D., Popović, Iva, Stepić, Milutin, Petković, Marijana, "Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):296-299,
https://doi.org/10.46793/ICCBI23.296R . .

TY  - JOUR
AU  - Houdova, Dominika
AU  - Popović, Iva A.
AU  - Dellinger, Thomas
AU  - Nešić, Maja D.
AU  - Petković, Marijana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10135
AB  - The endangered loggerhead sea turtles (Caretta caretta) are a relatively abundant sea turtle species in Madeiran waters, where they encounter various environmental stressors: from natural to anthropogenic. The physiological stress response is increased corticosterone (CS) level in the blood, achieving 2 ng/mL. Although there are various analytical methods for determining the CS concentration in turtle blood, most of them require tedious procedures for the preparation. In this work, we have tested the possibility of using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) for fast screening and detection of CS in blood from a turtle captured at the coast of Madeira Island. MALDI does not require sample derivatization and is suitable for low sample volume. The linearity, precision, and limit of detection and quantification with three organic MALDI matrices were investigated in this study. The signal-to-noise ratio of the CS-derived signal was used as a parameter for quantification. Isotopically labelled corticosterone was added as a correction factor at fixed concentration to achieve better linearity and precision. Our results demonstrate that this method has the potential for quantification of CS in turtle blood. However, the lowest concentration of CS extracted from plasma that was still detectable by MALDI TOF MS was about 0.04 mg/mL, which is about ten times higher than the expected CS concentration in blood.
T2  - International Journal of Mass Spectrometry
T1  - Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles
VL  - 473
SP  - 116796
DO  - 10.1016/j.ijms.2022.116796
ER  - 

@article{
author = "Houdova, Dominika and Popović, Iva A. and Dellinger, Thomas and Nešić, Maja D. and Petković, Marijana",
year = "2022",
abstract = "The endangered loggerhead sea turtles (Caretta caretta) are a relatively abundant sea turtle species in Madeiran waters, where they encounter various environmental stressors: from natural to anthropogenic. The physiological stress response is increased corticosterone (CS) level in the blood, achieving 2 ng/mL. Although there are various analytical methods for determining the CS concentration in turtle blood, most of them require tedious procedures for the preparation. In this work, we have tested the possibility of using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) for fast screening and detection of CS in blood from a turtle captured at the coast of Madeira Island. MALDI does not require sample derivatization and is suitable for low sample volume. The linearity, precision, and limit of detection and quantification with three organic MALDI matrices were investigated in this study. The signal-to-noise ratio of the CS-derived signal was used as a parameter for quantification. Isotopically labelled corticosterone was added as a correction factor at fixed concentration to achieve better linearity and precision. Our results demonstrate that this method has the potential for quantification of CS in turtle blood. However, the lowest concentration of CS extracted from plasma that was still detectable by MALDI TOF MS was about 0.04 mg/mL, which is about ten times higher than the expected CS concentration in blood.",
journal = "International Journal of Mass Spectrometry",
title = "Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles",
volume = "473",
pages = "116796",
doi = "10.1016/j.ijms.2022.116796"
}

Houdova, D., Popović, I. A., Dellinger, T., Nešić, M. D.,& Petković, M.. (2022). Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles. in International Journal of Mass Spectrometry, 473, 116796.
https://doi.org/10.1016/j.ijms.2022.116796

Houdova D, Popović IA, Dellinger T, Nešić MD, Petković M. Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles. in International Journal of Mass Spectrometry. 2022;473:116796.
doi:10.1016/j.ijms.2022.116796 .

Houdova, Dominika, Popović, Iva A., Dellinger, Thomas, Nešić, Maja D., Petković, Marijana, "Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles" in International Journal of Mass Spectrometry, 473 (2022):116796,
https://doi.org/10.1016/j.ijms.2022.116796 . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Algarra, Manuel
AU  - Popović, Iva A.
AU  - Stepić, Milutin
AU  - Gonçalves, Mara
AU  - Petković, Marijana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10184
AB  - In the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive understanding of the effect of the drug [Ru(η5-Cp)(PPh3)2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy. Techniques are used for screening the effect of potential metallodrug, RuCN, without and with drug carriers (carbon dots (CDs) and nitrogen-doped carbon dots (N-CDs)) on the lipids of the human ovarian cancer cell line A2780. MALDI TOF MS results revealed that the lysis of ovarian cancer membrane lipids is promoted by RuCN and not by drug carriers (CDs and N-CDs). Furthermore, SR-FTIR results strongly suggested that the phospholipids of cancer cells undergo oxidative stress after the treatment with RuCN that was accompanied by the disordering of the fatty acid chains. On the other hand, using (N-)CDs as RuCN nanocarriers prevented the oxidative stress caused by RuCN but did not prevent the disordering of the fatty acid chain packing. Finally, we demonstrated that RuCN and RuCN/(N-)CDs alter the hydration of the membrane surface in the membrane–water interface region.
T2  - Cancers
T1  - Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study
VL  - 14
IS  - 5
SP  - 1182
DO  - 10.3390/cancers14051182
ER  - 

@article{
author = "Nešić, Maja D. and Dučić, Tanja and Algarra, Manuel and Popović, Iva A. and Stepić, Milutin and Gonçalves, Mara and Petković, Marijana",
year = "2022",
abstract = "In the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive understanding of the effect of the drug [Ru(η5-Cp)(PPh3)2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy. Techniques are used for screening the effect of potential metallodrug, RuCN, without and with drug carriers (carbon dots (CDs) and nitrogen-doped carbon dots (N-CDs)) on the lipids of the human ovarian cancer cell line A2780. MALDI TOF MS results revealed that the lysis of ovarian cancer membrane lipids is promoted by RuCN and not by drug carriers (CDs and N-CDs). Furthermore, SR-FTIR results strongly suggested that the phospholipids of cancer cells undergo oxidative stress after the treatment with RuCN that was accompanied by the disordering of the fatty acid chains. On the other hand, using (N-)CDs as RuCN nanocarriers prevented the oxidative stress caused by RuCN but did not prevent the disordering of the fatty acid chain packing. Finally, we demonstrated that RuCN and RuCN/(N-)CDs alter the hydration of the membrane surface in the membrane–water interface region.",
journal = "Cancers",
title = "Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study",
volume = "14",
number = "5",
pages = "1182",
doi = "10.3390/cancers14051182"
}

Nešić, M. D., Dučić, T., Algarra, M., Popović, I. A., Stepić, M., Gonçalves, M.,& Petković, M.. (2022). Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study. in Cancers, 14(5), 1182.
https://doi.org/10.3390/cancers14051182

Nešić MD, Dučić T, Algarra M, Popović IA, Stepić M, Gonçalves M, Petković M. Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study. in Cancers. 2022;14(5):1182.
doi:10.3390/cancers14051182 .

Nešić, Maja D., Dučić, Tanja, Algarra, Manuel, Popović, Iva A., Stepić, Milutin, Gonçalves, Mara, Petković, Marijana, "Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study" in Cancers, 14, no. 5 (2022):1182,
https://doi.org/10.3390/cancers14051182 . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Algarra, Manuel
AU  - Soto, Juan
AU  - Nenadović, Miloš
AU  - Popović, Iva A.
AU  - Petković, Marijana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9930
AB  - Metal acetate complexes are highly interesting in designing and synthesizing metal–organic frameworks, which have potential applications in gas storage, molecular sieves, ion exchange, and catalysis. Cadmium is well recognized as an industrial pollutant and a food contaminant that severely affects human health. For these reasons, the detection and analysis of Cd are of great interest. A major challenge in studying cadmium compounds' structure is that Cd2+ can have a wide range of coordination numbers and diverse geometries. (MA)LDI is extremely useful for detecting Cd(OAc)2·2H2O and investigating Cd's complex and flexible nature. We applied LDI to detect and characterize the Cd-related clusters and MALDI approach to investigate the Cd(OAc)2x2H2O reactivity and detectability with the five different organic matrices. The results revealed that in the solid-state Cd(OAc)2x2H2O tend to form coordination polymers, and in the presence of coordinating ligands (matrix molecules) form smaller complexes. In addition, the correlation between Cd-related LOD and matrix features was observed and discussed, indicating that the LODs of this analyte strongly depend on the optical absorption of the matrix-analyte adduct.
T2  - Polyhedron
T1  - Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts
VL  - 209
SP  - 115463
DO  - 10.1016/j.poly.2021.115463
ER  - 

@article{
author = "Nešić, Maja D. and Algarra, Manuel and Soto, Juan and Nenadović, Miloš and Popović, Iva A. and Petković, Marijana",
year = "2021",
abstract = "Metal acetate complexes are highly interesting in designing and synthesizing metal–organic frameworks, which have potential applications in gas storage, molecular sieves, ion exchange, and catalysis. Cadmium is well recognized as an industrial pollutant and a food contaminant that severely affects human health. For these reasons, the detection and analysis of Cd are of great interest. A major challenge in studying cadmium compounds' structure is that Cd2+ can have a wide range of coordination numbers and diverse geometries. (MA)LDI is extremely useful for detecting Cd(OAc)2·2H2O and investigating Cd's complex and flexible nature. We applied LDI to detect and characterize the Cd-related clusters and MALDI approach to investigate the Cd(OAc)2x2H2O reactivity and detectability with the five different organic matrices. The results revealed that in the solid-state Cd(OAc)2x2H2O tend to form coordination polymers, and in the presence of coordinating ligands (matrix molecules) form smaller complexes. In addition, the correlation between Cd-related LOD and matrix features was observed and discussed, indicating that the LODs of this analyte strongly depend on the optical absorption of the matrix-analyte adduct.",
journal = "Polyhedron",
title = "Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts",
volume = "209",
pages = "115463",
doi = "10.1016/j.poly.2021.115463"
}

Nešić, M. D., Algarra, M., Soto, J., Nenadović, M., Popović, I. A.,& Petković, M.. (2021). Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts. in Polyhedron, 209, 115463.
https://doi.org/10.1016/j.poly.2021.115463

Nešić MD, Algarra M, Soto J, Nenadović M, Popović IA, Petković M. Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts. in Polyhedron. 2021;209:115463.
doi:10.1016/j.poly.2021.115463 .

Nešić, Maja D., Algarra, Manuel, Soto, Juan, Nenadović, Miloš, Popović, Iva A., Petković, Marijana, "Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts" in Polyhedron, 209 (2021):115463,
https://doi.org/10.1016/j.poly.2021.115463 . .

TY  - JOUR
AU  - Petković, Marijana
AU  - Leopold, Jenny
AU  - Popović, Iva A.
AU  - Dimić, Dušan
AU  - Ilić, Jelica
AU  - Nenadović, Miloš
AU  - Rakočević, Zlatko Lj.
AU  - Schiller, Jürgen
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8623
AB  - In this work, the performances of two ionic liquid matrices (ILMs) with the same ammonium counterpart for mass spectrometric analysis of the insoluble and soluble sucralfate were compared. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) was performed assisted by the butylammonium salts of α-cyano-hydroxycinnamic acid (CHCAB) and 2,5-dihydroxybenzoic acid (DHBB). CHCAB has a higher IE than DHBB, but better optical properties. CHCAB is more suitable for the analysis of sucralfate, although molecular ions of both compounds were detectable only with low intensities. Thus, optical properties of ILMs are crucial to enhance the sensitivity of MALDI MS detection of polysulfated oligosaccharides. © 2019, © 2019 Taylor & Francis Group, LLC.
T2  - Journal of Carbohydrate Chemistry
T1  - Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate
VL  - 39
IS  - 1
SP  - 1
EP  - 23
DO  - 10.1080/07328303.2019.1669633
ER  - 

@article{
author = "Petković, Marijana and Leopold, Jenny and Popović, Iva A. and Dimić, Dušan and Ilić, Jelica and Nenadović, Miloš and Rakočević, Zlatko Lj. and Schiller, Jürgen",
year = "2020",
abstract = "In this work, the performances of two ionic liquid matrices (ILMs) with the same ammonium counterpart for mass spectrometric analysis of the insoluble and soluble sucralfate were compared. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) was performed assisted by the butylammonium salts of α-cyano-hydroxycinnamic acid (CHCAB) and 2,5-dihydroxybenzoic acid (DHBB). CHCAB has a higher IE than DHBB, but better optical properties. CHCAB is more suitable for the analysis of sucralfate, although molecular ions of both compounds were detectable only with low intensities. Thus, optical properties of ILMs are crucial to enhance the sensitivity of MALDI MS detection of polysulfated oligosaccharides. © 2019, © 2019 Taylor & Francis Group, LLC.",
journal = "Journal of Carbohydrate Chemistry",
title = "Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate",
volume = "39",
number = "1",
pages = "1-23",
doi = "10.1080/07328303.2019.1669633"
}

Petković, M., Leopold, J., Popović, I. A., Dimić, D., Ilić, J., Nenadović, M., Rakočević, Z. Lj.,& Schiller, J.. (2020). Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate. in Journal of Carbohydrate Chemistry, 39(1), 1-23.
https://doi.org/10.1080/07328303.2019.1669633

Petković M, Leopold J, Popović IA, Dimić D, Ilić J, Nenadović M, Rakočević ZL, Schiller J. Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate. in Journal of Carbohydrate Chemistry. 2020;39(1):1-23.
doi:10.1080/07328303.2019.1669633 .

Petković, Marijana, Leopold, Jenny, Popović, Iva A., Dimić, Dušan, Ilić, Jelica, Nenadović, Miloš, Rakočević, Zlatko Lj., Schiller, Jürgen, "Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate" in Journal of Carbohydrate Chemistry, 39, no. 1 (2020):1-23,
https://doi.org/10.1080/07328303.2019.1669633 . .

TY  - CONF
AU  - Nešić, Maja D.
AU  - Stepić, Milutin
AU  - Korićanac, Lela
AU  - Radoičić, Marija
AU  - Šaponjić, Zoran
AU  - Popović, Iva
AU  - Petković, Marijana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11891
AB  - There has been a growing demand for development and improvement of cancer treatment in order to overcome disadvantages and limitations of traditional chemotherapy such as resistance, toxicity, and activity against a small number of tumor types. Photodynamic therapy is a rapidly developing cancer treatment that utilizes the combination of photoactive drug and/or carrier and light as an external stimulus to destroy tumors, achieve a maximum concentration of the drug on tumor site, reduce side effects of drug onto the healthy tissue, enable dosage control, and more effective treatment which could improve outcome of therapy [1]. We investigated possibility to use ТiО2 nanoparticles as a carrier for potential antitumor drug [Ru(II)(dcbpy)2Cl2], cis-dichlorobis (2, 2'-bipyridyl-4, 4'-dicarboxylicacid) ruthenium(II). Nanocomposite system has been formed by binding of ruthenium complex to the ТiО2 nanoparticles. Components of the system are selected according to their preferences, such as biocompatibility, photo activity, and easy surface modification for ТiО2, and less toxicity, significant activity against cancer metastases, and activity on tumors that were resistant to a variety of standard chemotherapeutic agents for Ru-complexes. Both components of the system are also photoactive, therefore potential for system manipulation and use in photodinamic therapy has been investigated by irradiation with UV and visible light. Additionally, the feasibility of using this system as a light-triggered drug delivery system was shown on amelanotic melanoma cancer line. The experimental results revealed a potential of nanocomposite system for long-term constant release of complex which is suitable for clinical practice. The further investigation of nanocomposite system indicated that it exhibited UV and red light susceptible drug release behavior [2]. More precisely, the system demonstrated slower complex release upon visible and increased release rate upon UV light illumination which was also in good correlation with the light–dependent cytotoxicity of the system demonstrated on amelanotic melanoma cancer line. The melanoma cancer cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system [3]. All obtained results suggested that nanocomposite system may have a potential use as a light sensitive drug delivery system in photodynamic therapy which could significantly contribute to development of more efficient and less invasive therapy of melanoma which is highly aggressive and deadliest form of skin cancer.
PB  - Belgrade : Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts
T1  - Development of photo-sensitive nanocomposite system for controlled metallo-drug delivery in skin cancer therapy
SP  - 39
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11891
ER  - 

@conference{
author = "Nešić, Maja D. and Stepić, Milutin and Korićanac, Lela and Radoičić, Marija and Šaponjić, Zoran and Popović, Iva and Petković, Marijana",
year = "2019",
abstract = "There has been a growing demand for development and improvement of cancer treatment in order to overcome disadvantages and limitations of traditional chemotherapy such as resistance, toxicity, and activity against a small number of tumor types. Photodynamic therapy is a rapidly developing cancer treatment that utilizes the combination of photoactive drug and/or carrier and light as an external stimulus to destroy tumors, achieve a maximum concentration of the drug on tumor site, reduce side effects of drug onto the healthy tissue, enable dosage control, and more effective treatment which could improve outcome of therapy [1]. We investigated possibility to use ТiО2 nanoparticles as a carrier for potential antitumor drug [Ru(II)(dcbpy)2Cl2], cis-dichlorobis (2, 2'-bipyridyl-4, 4'-dicarboxylicacid) ruthenium(II). Nanocomposite system has been formed by binding of ruthenium complex to the ТiО2 nanoparticles. Components of the system are selected according to their preferences, such as biocompatibility, photo activity, and easy surface modification for ТiО2, and less toxicity, significant activity against cancer metastases, and activity on tumors that were resistant to a variety of standard chemotherapeutic agents for Ru-complexes. Both components of the system are also photoactive, therefore potential for system manipulation and use in photodinamic therapy has been investigated by irradiation with UV and visible light. Additionally, the feasibility of using this system as a light-triggered drug delivery system was shown on amelanotic melanoma cancer line. The experimental results revealed a potential of nanocomposite system for long-term constant release of complex which is suitable for clinical practice. The further investigation of nanocomposite system indicated that it exhibited UV and red light susceptible drug release behavior [2]. More precisely, the system demonstrated slower complex release upon visible and increased release rate upon UV light illumination which was also in good correlation with the light–dependent cytotoxicity of the system demonstrated on amelanotic melanoma cancer line. The melanoma cancer cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system [3]. All obtained results suggested that nanocomposite system may have a potential use as a light sensitive drug delivery system in photodynamic therapy which could significantly contribute to development of more efficient and less invasive therapy of melanoma which is highly aggressive and deadliest form of skin cancer.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts",
title = "Development of photo-sensitive nanocomposite system for controlled metallo-drug delivery in skin cancer therapy",
pages = "39-39",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11891"
}

Nešić, M. D., Stepić, M., Korićanac, L., Radoičić, M., Šaponjić, Z., Popović, I.,& Petković, M.. (2019). Development of photo-sensitive nanocomposite system for controlled metallo-drug delivery in skin cancer therapy. in PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts
Belgrade : Vinča Institute of Nuclear Sciences, University of Belgrade., 39-39.
https://hdl.handle.net/21.15107/rcub_vinar_11891

Nešić MD, Stepić M, Korićanac L, Radoičić M, Šaponjić Z, Popović I, Petković M. Development of photo-sensitive nanocomposite system for controlled metallo-drug delivery in skin cancer therapy. in PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts. 2019;:39-39.
https://hdl.handle.net/21.15107/rcub_vinar_11891 .

Nešić, Maja D., Stepić, Milutin, Korićanac, Lela, Radoičić, Marija, Šaponjić, Zoran, Popović, Iva, Petković, Marijana, "Development of photo-sensitive nanocomposite system for controlled metallo-drug delivery in skin cancer therapy" in PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts (2019):39-39,
https://hdl.handle.net/21.15107/rcub_vinar_11891 .

TY  - CONF
AU  - Popović, Iva
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11862
AB  - MALDI TOF (Matrix-Assisted Laser Desorption and Ionization Time-Of-Flight) mass spectrometry is soft ionization technique. In order to prevent/reduce fragmentation of analyzed molecules, induced with direct excitation with laser, samples are mixed with matrix molecules. Matrix has high absorption coefficient in the range of the laser emission. In spite of numerous advantages, there are serious drawbacks of the matrices, especially for the analysis of molecules with low molecular mass (above 1000 Da). In this group of molecules there are various biologically active molecules, so there is a great need to overcome disadvantages of the application of organic matrices. Several alternative approaches have been developed: an organicmatrix-free approach in which the substrates, usually nanoparticles act as a matrix. The term SALDI (Surface-Assisted Laser Desorption and Ionization) was coined to designate the techniques that use nanostructured substrates. Nanoparticles absorb the laser energy and then rapidly transfer to analyzed molecule. Titanium (IV)-oxide (TiO2) is considered to be a good candidate for SALDI substrate since it is readily available, chemically stable, non-toxic and inexpensive material. TiO2 is a semiconductor with high absorptivity of UV light of nitrogen laser (have a large band gap 3.2 eV) which is used in MALDI TOF mass spectrometry, but the method of synthesis of TiO2 nanocrystal have a great impact on absorption because this phenomenon depends on size, shape and composition of nanoparticles. The applicability of TiO2 nanocrystals of different size and shape was tested. Colloidal TiO2 nanoparticles, TiO2 prolate nanospheroids and TiO2 nanotubes were used as substrates for potential SALDI TOF MS quantitative analysis of biologically active molecules with small molecule mass. First step to test efficiency of TiO2 nanocrystal for the mass spectrometry analysis of small molecules was to examine their qualitative characteristics as substrates. It is shown that they have high tolerance to increased concentration on inorganic salts and to high laser intensity. Next step was to examine qualitative characteristics of TiO2 nanocrystals substrates for mass spectrometric analysis based on following parameters: the homogeneity of the distribution of analyte over substrate surface in SALDI approach define reproducibility and accuracy of the measurement, within day precision to compare the accuracy of measurements with respect to signal intensity and potential use of tested systems for quantitative analysis, and day-to-day reproducibility. Based on all results, the great potential of TiO2 nanocrystals (especially for the TiO2 prolate nanospheroids) for the quantitative and qualitative analysis of biologicaly relevant small molecules (Mm less than 1000 Da) was demonstrated. [1-4]
PB  - Belgrade : Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts
T1  - Investigations of the application of inorganic substrates based on TiO2 nanocrystals for the detection and quantification of small molecules with SALDI TOF mass spectrometry
SP  - 38
EP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11862
ER  - 

@conference{
author = "Popović, Iva",
year = "2019",
abstract = "MALDI TOF (Matrix-Assisted Laser Desorption and Ionization Time-Of-Flight) mass spectrometry is soft ionization technique. In order to prevent/reduce fragmentation of analyzed molecules, induced with direct excitation with laser, samples are mixed with matrix molecules. Matrix has high absorption coefficient in the range of the laser emission. In spite of numerous advantages, there are serious drawbacks of the matrices, especially for the analysis of molecules with low molecular mass (above 1000 Da). In this group of molecules there are various biologically active molecules, so there is a great need to overcome disadvantages of the application of organic matrices. Several alternative approaches have been developed: an organicmatrix-free approach in which the substrates, usually nanoparticles act as a matrix. The term SALDI (Surface-Assisted Laser Desorption and Ionization) was coined to designate the techniques that use nanostructured substrates. Nanoparticles absorb the laser energy and then rapidly transfer to analyzed molecule. Titanium (IV)-oxide (TiO2) is considered to be a good candidate for SALDI substrate since it is readily available, chemically stable, non-toxic and inexpensive material. TiO2 is a semiconductor with high absorptivity of UV light of nitrogen laser (have a large band gap 3.2 eV) which is used in MALDI TOF mass spectrometry, but the method of synthesis of TiO2 nanocrystal have a great impact on absorption because this phenomenon depends on size, shape and composition of nanoparticles. The applicability of TiO2 nanocrystals of different size and shape was tested. Colloidal TiO2 nanoparticles, TiO2 prolate nanospheroids and TiO2 nanotubes were used as substrates for potential SALDI TOF MS quantitative analysis of biologically active molecules with small molecule mass. First step to test efficiency of TiO2 nanocrystal for the mass spectrometry analysis of small molecules was to examine their qualitative characteristics as substrates. It is shown that they have high tolerance to increased concentration on inorganic salts and to high laser intensity. Next step was to examine qualitative characteristics of TiO2 nanocrystals substrates for mass spectrometric analysis based on following parameters: the homogeneity of the distribution of analyte over substrate surface in SALDI approach define reproducibility and accuracy of the measurement, within day precision to compare the accuracy of measurements with respect to signal intensity and potential use of tested systems for quantitative analysis, and day-to-day reproducibility. Based on all results, the great potential of TiO2 nanocrystals (especially for the TiO2 prolate nanospheroids) for the quantitative and qualitative analysis of biologicaly relevant small molecules (Mm less than 1000 Da) was demonstrated. [1-4]",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts",
title = "Investigations of the application of inorganic substrates based on TiO2 nanocrystals for the detection and quantification of small molecules with SALDI TOF mass spectrometry",
pages = "38-38",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11862"
}

Popović, I.. (2019). Investigations of the application of inorganic substrates based on TiO2 nanocrystals for the detection and quantification of small molecules with SALDI TOF mass spectrometry. in PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts
Belgrade : Vinča Institute of Nuclear Sciences, University of Belgrade., 38-38.
https://hdl.handle.net/21.15107/rcub_vinar_11862

Popović I. Investigations of the application of inorganic substrates based on TiO2 nanocrystals for the detection and quantification of small molecules with SALDI TOF mass spectrometry. in PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts. 2019;:38-38.
https://hdl.handle.net/21.15107/rcub_vinar_11862 .

Popović, Iva, "Investigations of the application of inorganic substrates based on TiO2 nanocrystals for the detection and quantification of small molecules with SALDI TOF mass spectrometry" in PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts (2019):38-38,
https://hdl.handle.net/21.15107/rcub_vinar_11862 .

TY  - JOUR
AU  - Matijević, Milica
AU  - Popović, Iva A.
AU  - Stepić, Milutin
AU  - Nešić, Maja D.
AU  - Radoičić, Marija B.
AU  - Stanković, Maja
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9062
AB  - Among various nano-scaled materials composed from a spectrum of chemical compounds, inorganic nanoparticles are very attractive due to their physico-chemical properties, as well as their availability, simplicity, possibility of modifications, stability and biocompatibility. They are, on the one hand, an useful tool in advanced analytical chemistry, in particular for studying of biologically-relevant processes, but also important as functional parts of the systems designed for controlled and targeted delivery of medicaments for treatment of a variety of diseases and for imaging. So far, thousands of compounds and systems have been developed for the above-mentioned purposes, but there are only a few reviews dealing with these topics. The aim of this review is, thus, to summarize recent applications of nano-structured inorganic materials in the field of drug delivery, bioimaging and bioanalytics, and to give a prospective from the standpoint of biology-related applications.
T2  - Biologica Nyssana
T1  - Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics
VL  - 9
IS  - 1
SP  - 1
EP  - 19
DO  - 10.5281/zenodo.1470841
ER  - 

@article{
author = "Matijević, Milica and Popović, Iva A. and Stepić, Milutin and Nešić, Maja D. and Radoičić, Marija B. and Stanković, Maja and Šaponjić, Zoran and Petković, Marijana",
year = "2018",
abstract = "Among various nano-scaled materials composed from a spectrum of chemical compounds, inorganic nanoparticles are very attractive due to their physico-chemical properties, as well as their availability, simplicity, possibility of modifications, stability and biocompatibility. They are, on the one hand, an useful tool in advanced analytical chemistry, in particular for studying of biologically-relevant processes, but also important as functional parts of the systems designed for controlled and targeted delivery of medicaments for treatment of a variety of diseases and for imaging. So far, thousands of compounds and systems have been developed for the above-mentioned purposes, but there are only a few reviews dealing with these topics. The aim of this review is, thus, to summarize recent applications of nano-structured inorganic materials in the field of drug delivery, bioimaging and bioanalytics, and to give a prospective from the standpoint of biology-related applications.",
journal = "Biologica Nyssana",
title = "Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics",
volume = "9",
number = "1",
pages = "1-19",
doi = "10.5281/zenodo.1470841"
}

Matijević, M., Popović, I. A., Stepić, M., Nešić, M. D., Radoičić, M. B., Stanković, M., Šaponjić, Z.,& Petković, M.. (2018). Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics. in Biologica Nyssana, 9(1), 1-19.
https://doi.org/10.5281/zenodo.1470841

Matijević M, Popović IA, Stepić M, Nešić MD, Radoičić MB, Stanković M, Šaponjić Z, Petković M. Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics. in Biologica Nyssana. 2018;9(1):1-19.
doi:10.5281/zenodo.1470841 .

Matijević, Milica, Popović, Iva A., Stepić, Milutin, Nešić, Maja D., Radoičić, Marija B., Stanković, Maja, Šaponjić, Zoran, Petković, Marijana, "Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics" in Biologica Nyssana, 9, no. 1 (2018):1-19,
https://doi.org/10.5281/zenodo.1470841 . .

TY  - JOUR
AU  - Rakić-Kostić, Tijana M.
AU  - Bogojeski, Jovana V.
AU  - Popović, Iva A.
AU  - Nešić, Maja D.
AU  - Rajčić, Boris
AU  - Nišavić, Marija
AU  - Petković, Marijana
AU  - Veličković, Suzana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1362
AB  - This paper presents optimization of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer (MS) instrumental parameters for the analysis of chloro(2,2 , 2 -terpyridine) palladium(II) chloride dihydrate complex applying design of experiments methodology (DoE). This complex is of interest for potential use in the cancer therapy. DoE methodology was proved to succeed in optimization of many complex analytical problems. However, it has been poorly used for MALDI-TOF-MS optimization up to now. The theoretical mathematical relationships which explain the influence of important experimental factors (laser energy, grid voltage and number of laser shots) on the selected responses (signal to noise - S/N ratio and the resolution - R of the leading peak) is established. The optimal instrumental settings providing maximal S/N and R are identified and experimentally verified.
T2  - Hemijska industrija
T1  - Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes
VL  - 71
IS  - 4
SP  - 281
EP  - 288
DO  - 10.2298/HEMIND160614038R
ER  - 

@article{
author = "Rakić-Kostić, Tijana M. and Bogojeski, Jovana V. and Popović, Iva A. and Nešić, Maja D. and Rajčić, Boris and Nišavić, Marija and Petković, Marijana and Veličković, Suzana",
year = "2017",
abstract = "This paper presents optimization of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer (MS) instrumental parameters for the analysis of chloro(2,2 , 2 -terpyridine) palladium(II) chloride dihydrate complex applying design of experiments methodology (DoE). This complex is of interest for potential use in the cancer therapy. DoE methodology was proved to succeed in optimization of many complex analytical problems. However, it has been poorly used for MALDI-TOF-MS optimization up to now. The theoretical mathematical relationships which explain the influence of important experimental factors (laser energy, grid voltage and number of laser shots) on the selected responses (signal to noise - S/N ratio and the resolution - R of the leading peak) is established. The optimal instrumental settings providing maximal S/N and R are identified and experimentally verified.",
journal = "Hemijska industrija",
title = "Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes",
volume = "71",
number = "4",
pages = "281-288",
doi = "10.2298/HEMIND160614038R"
}

Rakić-Kostić, T. M., Bogojeski, J. V., Popović, I. A., Nešić, M. D., Rajčić, B., Nišavić, M., Petković, M.,& Veličković, S.. (2017). Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes. in Hemijska industrija, 71(4), 281-288.
https://doi.org/10.2298/HEMIND160614038R

Rakić-Kostić TM, Bogojeski JV, Popović IA, Nešić MD, Rajčić B, Nišavić M, Petković M, Veličković S. Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes. in Hemijska industrija. 2017;71(4):281-288.
doi:10.2298/HEMIND160614038R .

Rakić-Kostić, Tijana M., Bogojeski, Jovana V., Popović, Iva A., Nešić, Maja D., Rajčić, Boris, Nišavić, Marija, Petković, Marijana, Veličković, Suzana, "Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes" in Hemijska industrija, 71, no. 4 (2017):281-288,
https://doi.org/10.2298/HEMIND160614038R . .

TY  - JOUR
AU  - Kamčeva, Tina T.
AU  - Nešić, Maja D.
AU  - Stoiljković, Milovan
AU  - Popović, Iva A.
AU  - Miletić, Jadranka
AU  - Rajčić, Boris
AU  - Petković, Marijana
AU  - Veličković, Suzana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1471
AB  - In this work it has been shown that both the laser desorption/ionization mass spectrometry (LDI MS) and the matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) are the simple and quick methods for determination of relative natural isotopic distribution of lead. The analysis of metal salts with these approaches does not require any time-consuming preparation of samples: a single run can take only a minute, and numerous information can be obtained. Results obtained in this work show that chosen matrix has no negative effect on quantitative determination of lead isotopes and support once more the applicability of MALDI TOF MS for lead isotope distribution determination in the sample and accurate data are obtained. Additionally, the generation of PbnOn and PbnOn-1 (n: 2-6) clusters have been successfully achieved in the positive mode, using the both LDI and MALDI methods. All stoichiometries were confirmed using isotopic pattern modelling.
T2  - Hemijska industrija
T1  - Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry
VL  - 71
IS  - 1
SP  - 19
EP  - 26
DO  - 10.2298/HEMIND151218013K
ER  - 

@article{
author = "Kamčeva, Tina T. and Nešić, Maja D. and Stoiljković, Milovan and Popović, Iva A. and Miletić, Jadranka and Rajčić, Boris and Petković, Marijana and Veličković, Suzana",
year = "2017",
abstract = "In this work it has been shown that both the laser desorption/ionization mass spectrometry (LDI MS) and the matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) are the simple and quick methods for determination of relative natural isotopic distribution of lead. The analysis of metal salts with these approaches does not require any time-consuming preparation of samples: a single run can take only a minute, and numerous information can be obtained. Results obtained in this work show that chosen matrix has no negative effect on quantitative determination of lead isotopes and support once more the applicability of MALDI TOF MS for lead isotope distribution determination in the sample and accurate data are obtained. Additionally, the generation of PbnOn and PbnOn-1 (n: 2-6) clusters have been successfully achieved in the positive mode, using the both LDI and MALDI methods. All stoichiometries were confirmed using isotopic pattern modelling.",
journal = "Hemijska industrija",
title = "Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry",
volume = "71",
number = "1",
pages = "19-26",
doi = "10.2298/HEMIND151218013K"
}

Kamčeva, T. T., Nešić, M. D., Stoiljković, M., Popović, I. A., Miletić, J., Rajčić, B., Petković, M.,& Veličković, S.. (2017). Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry. in Hemijska industrija, 71(1), 19-26.
https://doi.org/10.2298/HEMIND151218013K

Kamčeva TT, Nešić MD, Stoiljković M, Popović IA, Miletić J, Rajčić B, Petković M, Veličković S. Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry. in Hemijska industrija. 2017;71(1):19-26.
doi:10.2298/HEMIND151218013K .

Kamčeva, Tina T., Nešić, Maja D., Stoiljković, Milovan, Popović, Iva A., Miletić, Jadranka, Rajčić, Boris, Petković, Marijana, Veličković, Suzana, "Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry" in Hemijska industrija, 71, no. 1 (2017):19-26,
https://doi.org/10.2298/HEMIND151218013K . .

TY  - JOUR
AU  - Nešić, Maja A.
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Stepić, Milutin
AU  - Radoičić, Marija B.
AU  - Popović, Iva A.
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1747
AB  - We studied the colloidal TiO2 nanoparticles as a carrier for controlled delivery of the ruthenium complex to the melanoma cell line. The system demonstrated slower complex release upon visible and increased release rate upon UV light illumination. Accordingly, the light-dependent cytotoxicity of the system was demonstrated on amelanotic melanoma cancer line. The cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system. Both components of the system may act as photosensitizers, by generating reactive oxygen species, which promote cell death. Thus, the system might act dually, as photodynamic therapeutic agent and as the light tunable system for metallo-drug delivery and it might be of interest for development of new more efficient drug delivery approaches by using a light as external stimulus. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Journal of Photochemistry and Photobiology. A: Chemistry
T1  - Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex
VL  - 347
SP  - 55
EP  - 66
DO  - 10.1016/jjphotochem.2017.06.045
ER  - 

@article{
author = "Nešić, Maja A. and Žakula, Jelena and Korićanac, Lela and Stepić, Milutin and Radoičić, Marija B. and Popović, Iva A. and Šaponjić, Zoran and Petković, Marijana",
year = "2017",
abstract = "We studied the colloidal TiO2 nanoparticles as a carrier for controlled delivery of the ruthenium complex to the melanoma cell line. The system demonstrated slower complex release upon visible and increased release rate upon UV light illumination. Accordingly, the light-dependent cytotoxicity of the system was demonstrated on amelanotic melanoma cancer line. The cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system. Both components of the system may act as photosensitizers, by generating reactive oxygen species, which promote cell death. Thus, the system might act dually, as photodynamic therapeutic agent and as the light tunable system for metallo-drug delivery and it might be of interest for development of new more efficient drug delivery approaches by using a light as external stimulus. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Journal of Photochemistry and Photobiology. A: Chemistry",
title = "Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex",
volume = "347",
pages = "55-66",
doi = "10.1016/jjphotochem.2017.06.045"
}

Nešić, M. A., Žakula, J., Korićanac, L., Stepić, M., Radoičić, M. B., Popović, I. A., Šaponjić, Z.,& Petković, M.. (2017). Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex. in Journal of Photochemistry and Photobiology. A: Chemistry, 347, 55-66.
https://doi.org/10.1016/jjphotochem.2017.06.045

Nešić MA, Žakula J, Korićanac L, Stepić M, Radoičić MB, Popović IA, Šaponjić Z, Petković M. Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex. in Journal of Photochemistry and Photobiology. A: Chemistry. 2017;347:55-66.
doi:10.1016/jjphotochem.2017.06.045 .

Nešić, Maja A., Žakula, Jelena, Korićanac, Lela, Stepić, Milutin, Radoičić, Marija B., Popović, Iva A., Šaponjić, Zoran, Petković, Marijana, "Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex" in Journal of Photochemistry and Photobiology. A: Chemistry, 347 (2017):55-66,
https://doi.org/10.1016/jjphotochem.2017.06.045 . .

TY  - THES
AU  - Popović, Iva A.
PY  - 2017
UR  - http://eteze.kg.ac.rs/application/showtheses?thesesId=4617
UR  - https://fedorakg.kg.ac.rs/fedora/get/o:752/bdef:Content/download
UR  - http://nardus.mpn.gov.rs/123456789/7630
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7310
AB  - Izvod: MALDI TOF (Matrix Assisted Laser Desorption and Ionization Time-Of-Flight) masenaspektrometrija je meka jonizaciona tehnika koja se prvenstveno koristi za analizubiomolekula (proteina i nukleinskih kiselina) i omogućava detekciju molekula uzveoma nizak stepen fragmentacije. Kako bi se sprečila/ublažila fragmentacijaizazvana direktnom ekscitacijom laserom, uzorak se meša sa matricama koje imajuvisok apsorpcioni koeficijent u oblasti u kojoj laser emituje. Primarno su sekoristile organske, „tradicionalne“ matrice. Pored brojnih prednosti, organskematrice pokazuju i značajne mane koje onemogućavaju primenu MALDI masenespektrometrije za kvantitativnu analizu molekula i sprečavaju detekciju molekulamalih masa manjih od 1000 Da. Kako se u ovoj grupi molekula nalaze i različitibiološki značajni molekuli (metaboliti) postoji potreba za prevazilaženjemnedostataka organskih matrica.Jedan od najranije primenjenih načina je zamena organskih matrica neorganskimjedinjenjima i/ili nanočesticama (supstratima). Koriste se različiti supstrati nabazi ugljeničnih polimera i sol-gel polimerne strukture na bazi silicijum dioksida.Upotreba nanostrukturnih supstrata u metodi koja je nazvana SALDI (Surface AssistedLaser Desorption and Ionization) u poslednjoj deceniji je predmet velikog brojapublikovanih radova. Međutim, nanočestice od metala i oksida metala, kao što su Au,Pt, Ag, ZnO, Fe i MnO2/MnO3, nude prednost u odnosu na ostale materijale - stabilnijesu u vazduhu i poseduju visoku provodljivost. Prednost nanočestica je apsorpcijaenergije lasera i efikasan prenos energije do uzorka. Istakle su se nanočestice odtitan(IV)-oksida zbog svoje dostupnosti, netoksičnosti i niske cene, što je takođepredmet mnogih publikovanih radova. Osim navedenih osobina, titan(IV)-oksid jepoluprovodnik sa dobrom UV apsorpcijom. Generalno, TiO2 snažno apsorbuje UVsvetlost (ima znatnu širinu energetske barijere, 3,2 eV) azotnog lasera koji se koristiu MALDI TOF masenoj spektrometriji, ali proces pripreme nanočestica TiO2verovatno ima snažan uticaj na apsorpciju jer ona zavisi od veličine, oblika i sastavačestica. Površina, a posredno i veličina i oblik kristala utiču na analitičkeperformanse i jonizacionu efikasnost u SALDI masenoj spektrometriji.U ovoj doktorskoj disertaciji korišćeni su nanokristali TiO2 različite veličinei oblika: koloidne nanočestice TiO2 (TiO2 NČ) prosečnog dijametra 5 nm, elipsoidnenanočestice TiO2 (TiO2 ENČ) dužine 40-50 nm, lateralne dimenzije 14-16 nm inanotube TiO2 (TiO2 NT) dužine od 100 do 150 nm i prosečnog dijametra od 11 nm.Molekuli na kojima je bila testirana potencijalna primena nanokristala su biološkiaktivni molekuli malih masa: amino-kiseline (L-cistein, L-alanin, DL-metionin),tripeptid glutation, polni steroidni hormoni (estradiol, testosteron, progesteron),ugljeni hidrati (D-(+)-glukoza, D-(+)-maltoza, rafinoza, arabinoza, β-ciklodekstrin),limunska kiselina, deksametazon (deksazon), vitamini (vitamin A i vitamin E).
AB  - Summary:MALDI TOF (Matrix-Assisted Laser Desorption and Ionization Time-Of-Flight) massspectrometry is soft ionization technique. Primarily, this technique was used for the analysis ofbiomolecules such as proteins and nucleic acids with low level of their fragmentation. In orderto prevent/reduce fragmentation of analyzed molecules, induced with direct excitation withlaser, samples are mixed with matrix molecules. Matrix has high absorption coefficient in therange of the laser emission. In spite of numerous advantages, there are serious drawbacks ofthe matrices, and because of that they cannot be used for quantitative MALDI massspectrometric analysis and for the detection of small molecules (molecular mass less than 1000Da). In this group of molecules (mass less than 1000 Da) there are various biologically activemolecules (metabolites), so there is a great need to overcome disadvantages of the applicationof organic matrices.Considerable efforts have been made to overcome the above mention problems andseveral alternative approaches have been developed: an organic-matrix-free approach in whichthe substrates, usually nanoparticles act as a matrix. Many are in use: graphite, silica gel, carbonpowder, activated carbon, graphene, porous silicon, and many more.The term SALDI (Surface-Assisted Laser Desorption and Ionization) was coined todesignate the techniques that use nanostructured substrates. The use of various nanoparticlesas substrates in SALDI MS has attracted a lot of attention in the last decade. Nanoparticlesabsorb the laser energy and then rapidly transfer to analyzed molecule. However, the substratesprepared from materials based on metal-oxides (Аu, Pt, Ag, ZnO, Fe and MnO2/MnO3) aremore stable in the air and have a high conductivity. Titanium(IV)-oxide (TiO2) is consideredto be a good candidate for SALDI substrate since it is readily available, chemically stable, nontoxicand inexpensive material. Titanium(IV)-oxide is a semiconductor with high absorptivityof UV light of nitrogen laser (have a large band gap 3.2 eV) which is used in MALDI TOFmass spectrometry, but the method of synthesis of TiO2 nanocrystal have a great impact onabsorption because this phenomena depends on size, shape and composition of nanoparticles.In this doctoral thesis, the applicability of TiO2 nanocrystals of different size and shape wastested. Colloidal TiO2 nanoparticles (NPs, average diameter ~ 5 nm), TiO2 prolatenanospheroids (PNSs, length: 40–50 nm, the lateral dimension: 14–16 nm) and TiO2 nanotubes(NTs, length: 100-150 nm, average diameter 11 nm) were used as substrates for potentialSALDI TOF MS quantitative analysis of low mass molecules. The analyzed molecules werebiologically active molecules with small molecule mass (Mm less than 1000 Da): amino acids(L-cysteine, L-alanine, DL-methionine), tripeptide glutathione, sex steroid hormones (estradiol,testosterone, progesterone), carbohydrates (D-(+)-glucose, D-(+)-maltose, raffinose, arabinose,β-cyclodextrin), citric acid, dexamethasone (dexasone), vitamins (vitamin А and vitamin Е).
PB  - Универзитет у Крагујевцу, Природно-математички факултет
T2  - Универзитет у Крагујевцу
T1  - Ispitivanje primene neorganskih supstrata na bazi nanokristala TiO2 za detekciju i kvantifikaciju malih molekula SALDI TOF masenom spektrometrijom
UR  - https://hdl.handle.net/21.15107/rcub_nardus_7630
ER  - 

@phdthesis{
author = "Popović, Iva A.",
year = "2017",
abstract = "Izvod: MALDI TOF (Matrix Assisted Laser Desorption and Ionization Time-Of-Flight) masenaspektrometrija je meka jonizaciona tehnika koja se prvenstveno koristi za analizubiomolekula (proteina i nukleinskih kiselina) i omogućava detekciju molekula uzveoma nizak stepen fragmentacije. Kako bi se sprečila/ublažila fragmentacijaizazvana direktnom ekscitacijom laserom, uzorak se meša sa matricama koje imajuvisok apsorpcioni koeficijent u oblasti u kojoj laser emituje. Primarno su sekoristile organske, „tradicionalne“ matrice. Pored brojnih prednosti, organskematrice pokazuju i značajne mane koje onemogućavaju primenu MALDI masenespektrometrije za kvantitativnu analizu molekula i sprečavaju detekciju molekulamalih masa manjih od 1000 Da. Kako se u ovoj grupi molekula nalaze i različitibiološki značajni molekuli (metaboliti) postoji potreba za prevazilaženjemnedostataka organskih matrica.Jedan od najranije primenjenih načina je zamena organskih matrica neorganskimjedinjenjima i/ili nanočesticama (supstratima). Koriste se različiti supstrati nabazi ugljeničnih polimera i sol-gel polimerne strukture na bazi silicijum dioksida.Upotreba nanostrukturnih supstrata u metodi koja je nazvana SALDI (Surface AssistedLaser Desorption and Ionization) u poslednjoj deceniji je predmet velikog brojapublikovanih radova. Međutim, nanočestice od metala i oksida metala, kao što su Au,Pt, Ag, ZnO, Fe i MnO2/MnO3, nude prednost u odnosu na ostale materijale - stabilnijesu u vazduhu i poseduju visoku provodljivost. Prednost nanočestica je apsorpcijaenergije lasera i efikasan prenos energije do uzorka. Istakle su se nanočestice odtitan(IV)-oksida zbog svoje dostupnosti, netoksičnosti i niske cene, što je takođepredmet mnogih publikovanih radova. Osim navedenih osobina, titan(IV)-oksid jepoluprovodnik sa dobrom UV apsorpcijom. Generalno, TiO2 snažno apsorbuje UVsvetlost (ima znatnu širinu energetske barijere, 3,2 eV) azotnog lasera koji se koristiu MALDI TOF masenoj spektrometriji, ali proces pripreme nanočestica TiO2verovatno ima snažan uticaj na apsorpciju jer ona zavisi od veličine, oblika i sastavačestica. Površina, a posredno i veličina i oblik kristala utiču na analitičkeperformanse i jonizacionu efikasnost u SALDI masenoj spektrometriji.U ovoj doktorskoj disertaciji korišćeni su nanokristali TiO2 različite veličinei oblika: koloidne nanočestice TiO2 (TiO2 NČ) prosečnog dijametra 5 nm, elipsoidnenanočestice TiO2 (TiO2 ENČ) dužine 40-50 nm, lateralne dimenzije 14-16 nm inanotube TiO2 (TiO2 NT) dužine od 100 do 150 nm i prosečnog dijametra od 11 nm.Molekuli na kojima je bila testirana potencijalna primena nanokristala su biološkiaktivni molekuli malih masa: amino-kiseline (L-cistein, L-alanin, DL-metionin),tripeptid glutation, polni steroidni hormoni (estradiol, testosteron, progesteron),ugljeni hidrati (D-(+)-glukoza, D-(+)-maltoza, rafinoza, arabinoza, β-ciklodekstrin),limunska kiselina, deksametazon (deksazon), vitamini (vitamin A i vitamin E)., Summary:MALDI TOF (Matrix-Assisted Laser Desorption and Ionization Time-Of-Flight) massspectrometry is soft ionization technique. Primarily, this technique was used for the analysis ofbiomolecules such as proteins and nucleic acids with low level of their fragmentation. In orderto prevent/reduce fragmentation of analyzed molecules, induced with direct excitation withlaser, samples are mixed with matrix molecules. Matrix has high absorption coefficient in therange of the laser emission. In spite of numerous advantages, there are serious drawbacks ofthe matrices, and because of that they cannot be used for quantitative MALDI massspectrometric analysis and for the detection of small molecules (molecular mass less than 1000Da). In this group of molecules (mass less than 1000 Da) there are various biologically activemolecules (metabolites), so there is a great need to overcome disadvantages of the applicationof organic matrices.Considerable efforts have been made to overcome the above mention problems andseveral alternative approaches have been developed: an organic-matrix-free approach in whichthe substrates, usually nanoparticles act as a matrix. Many are in use: graphite, silica gel, carbonpowder, activated carbon, graphene, porous silicon, and many more.The term SALDI (Surface-Assisted Laser Desorption and Ionization) was coined todesignate the techniques that use nanostructured substrates. The use of various nanoparticlesas substrates in SALDI MS has attracted a lot of attention in the last decade. Nanoparticlesabsorb the laser energy and then rapidly transfer to analyzed molecule. However, the substratesprepared from materials based on metal-oxides (Аu, Pt, Ag, ZnO, Fe and MnO2/MnO3) aremore stable in the air and have a high conductivity. Titanium(IV)-oxide (TiO2) is consideredto be a good candidate for SALDI substrate since it is readily available, chemically stable, nontoxicand inexpensive material. Titanium(IV)-oxide is a semiconductor with high absorptivityof UV light of nitrogen laser (have a large band gap 3.2 eV) which is used in MALDI TOFmass spectrometry, but the method of synthesis of TiO2 nanocrystal have a great impact onabsorption because this phenomena depends on size, shape and composition of nanoparticles.In this doctoral thesis, the applicability of TiO2 nanocrystals of different size and shape wastested. Colloidal TiO2 nanoparticles (NPs, average diameter ~ 5 nm), TiO2 prolatenanospheroids (PNSs, length: 40–50 nm, the lateral dimension: 14–16 nm) and TiO2 nanotubes(NTs, length: 100-150 nm, average diameter 11 nm) were used as substrates for potentialSALDI TOF MS quantitative analysis of low mass molecules. The analyzed molecules werebiologically active molecules with small molecule mass (Mm less than 1000 Da): amino acids(L-cysteine, L-alanine, DL-methionine), tripeptide glutathione, sex steroid hormones (estradiol,testosterone, progesterone), carbohydrates (D-(+)-glucose, D-(+)-maltose, raffinose, arabinose,β-cyclodextrin), citric acid, dexamethasone (dexasone), vitamins (vitamin А and vitamin Е).",
publisher = "Универзитет у Крагујевцу, Природно-математички факултет",
journal = "Универзитет у Крагујевцу",
title = "Ispitivanje primene neorganskih supstrata na bazi nanokristala TiO2 za detekciju i kvantifikaciju malih molekula SALDI TOF masenom spektrometrijom",
url = "https://hdl.handle.net/21.15107/rcub_nardus_7630"
}

Popović, I. A.. (2017). Ispitivanje primene neorganskih supstrata na bazi nanokristala TiO2 za detekciju i kvantifikaciju malih molekula SALDI TOF masenom spektrometrijom. in Универзитет у Крагујевцу
Универзитет у Крагујевцу, Природно-математички факултет..
https://hdl.handle.net/21.15107/rcub_nardus_7630

Popović IA. Ispitivanje primene neorganskih supstrata na bazi nanokristala TiO2 za detekciju i kvantifikaciju malih molekula SALDI TOF masenom spektrometrijom. in Универзитет у Крагујевцу. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_7630 .

Popović, Iva A., "Ispitivanje primene neorganskih supstrata na bazi nanokristala TiO2 za detekciju i kvantifikaciju malih molekula SALDI TOF masenom spektrometrijom" in Универзитет у Крагујевцу (2017),
https://hdl.handle.net/21.15107/rcub_nardus_7630 .

TY  - JOUR
AU  - Popović, Iva A.
AU  - Nešić, Maja D.
AU  - Vranješ, Mila
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/886
AB  - In the present study, the possibility for the application of TiO2 nanocrystals of various shapes and sizes, for substrate-assisted laser desorption and ionization time-of-flight mass spectrometric (SALDI TOF MS) quantitative analysis of small molecules (steroid hormones, amino acids and saccharides) was investigated. Parameters, such as homogeneity of the substrate/analyte distribution, reproducibility of the measurements, within-day, and day-to-day repeatability, were determined. The homogeneity of different nanocrystal/analyte combinations on the target plate were compared based on the signal-to-noise values of several analyte signals. Obtained results show that all TiO2 nanocrystals, regardless of their shape, have great potential for the detection and determination of steroid hormones, amino acids and saccharides with good analytical parameters and detection limits. On the other hand, the reproducibility of the S/N ratio and detectability of the analytes recorded in various modes differ depending on the substrate. All examined molecules were detectable in negative ion mode with TiO2 NTs, in contrast to all other organic matrices and substrates, and the best reproducibility was obtained with the larger nanocrystals, TiO2 PNSs and TiO2 NTs, making them good candidates for the quantitative determination of small molecules.
T2  - RSC Advances
T1  - TiO2 nanocrystals - assisted laser desorption and ionization time-of-flight mass spectrometric analysis of steroid hormones, amino acids and saccharides. Validation and comparison of methods
VL  - 6
IS  - 2
SP  - 1027
EP  - 1036
DO  - 10.1039/c5ra20042c
ER  - 

@article{
author = "Popović, Iva A. and Nešić, Maja D. and Vranješ, Mila and Šaponjić, Zoran and Petković, Marijana",
year = "2016",
abstract = "In the present study, the possibility for the application of TiO2 nanocrystals of various shapes and sizes, for substrate-assisted laser desorption and ionization time-of-flight mass spectrometric (SALDI TOF MS) quantitative analysis of small molecules (steroid hormones, amino acids and saccharides) was investigated. Parameters, such as homogeneity of the substrate/analyte distribution, reproducibility of the measurements, within-day, and day-to-day repeatability, were determined. The homogeneity of different nanocrystal/analyte combinations on the target plate were compared based on the signal-to-noise values of several analyte signals. Obtained results show that all TiO2 nanocrystals, regardless of their shape, have great potential for the detection and determination of steroid hormones, amino acids and saccharides with good analytical parameters and detection limits. On the other hand, the reproducibility of the S/N ratio and detectability of the analytes recorded in various modes differ depending on the substrate. All examined molecules were detectable in negative ion mode with TiO2 NTs, in contrast to all other organic matrices and substrates, and the best reproducibility was obtained with the larger nanocrystals, TiO2 PNSs and TiO2 NTs, making them good candidates for the quantitative determination of small molecules.",
journal = "RSC Advances",
title = "TiO2 nanocrystals - assisted laser desorption and ionization time-of-flight mass spectrometric analysis of steroid hormones, amino acids and saccharides. Validation and comparison of methods",
volume = "6",
number = "2",
pages = "1027-1036",
doi = "10.1039/c5ra20042c"
}

Popović, I. A., Nešić, M. D., Vranješ, M., Šaponjić, Z.,& Petković, M.. (2016). TiO2 nanocrystals - assisted laser desorption and ionization time-of-flight mass spectrometric analysis of steroid hormones, amino acids and saccharides. Validation and comparison of methods. in RSC Advances, 6(2), 1027-1036.
https://doi.org/10.1039/c5ra20042c

Popović IA, Nešić MD, Vranješ M, Šaponjić Z, Petković M. TiO2 nanocrystals - assisted laser desorption and ionization time-of-flight mass spectrometric analysis of steroid hormones, amino acids and saccharides. Validation and comparison of methods. in RSC Advances. 2016;6(2):1027-1036.
doi:10.1039/c5ra20042c .

Popović, Iva A., Nešić, Maja D., Vranješ, Mila, Šaponjić, Zoran, Petković, Marijana, "TiO2 nanocrystals - assisted laser desorption and ionization time-of-flight mass spectrometric analysis of steroid hormones, amino acids and saccharides. Validation and comparison of methods" in RSC Advances, 6, no. 2 (2016):1027-1036,
https://doi.org/10.1039/c5ra20042c . .

TY  - JOUR
AU  - Popović, Iva A.
AU  - Milovanović, Dubravka S.
AU  - Miletić, Jadranka
AU  - Nešić, Maja D.
AU  - Vranješ, Mila
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/902
AB  - Titanium dioxide is widely used substrate for laser desorption and ionization [(SA)LDI] mass spectrometry. As the semiconductor with high UV absorbance and rapid energy transfer to analytes, TiO2 provides soft and efficient desorption/ionization of analytes with relatively low degrees of fragmentation in the gas phase. TiO2 nanocrystals are convenient for small molecules (molecular mass LT 1000 Da) analysis due to low number of background signals. Desorption/ionization processes on TiO2 nanocrystals are related to the physical properties of the substrate, such as the ability to absorb and dissipate energy from the irradiating laser light source. In addition, size and shape of nanocrystals also affect these abilities. The aim of this study is to investigate the influence of shape and size dependant TiO2 nanocrystals/UV laser interaction on the quality of the mass spectra, detectability of analyzed molecules, intensities of the signals and homogeneity of co-crystals onto the MALDI plate, of carbohydrates. It is shown that the size and shape of nanocrystals influenced the way of packing carbohydrates onto plate, and thus affects homogeneity and reproducibility of mass spectrometry analysis.
PB  - Springer
T2  - Optical and Quantum Electronics
T1  - Dependence of the quality of SALDI TOF MS analysis on the TiO2 nanocrystals size and shape
VL  - 48
IS  - 2
DO  - 10.1007/s11082-016-0413-5
ER  - 

@article{
author = "Popović, Iva A. and Milovanović, Dubravka S. and Miletić, Jadranka and Nešić, Maja D. and Vranješ, Mila and Šaponjić, Zoran and Petković, Marijana",
year = "2016",
abstract = "Titanium dioxide is widely used substrate for laser desorption and ionization [(SA)LDI] mass spectrometry. As the semiconductor with high UV absorbance and rapid energy transfer to analytes, TiO2 provides soft and efficient desorption/ionization of analytes with relatively low degrees of fragmentation in the gas phase. TiO2 nanocrystals are convenient for small molecules (molecular mass LT 1000 Da) analysis due to low number of background signals. Desorption/ionization processes on TiO2 nanocrystals are related to the physical properties of the substrate, such as the ability to absorb and dissipate energy from the irradiating laser light source. In addition, size and shape of nanocrystals also affect these abilities. The aim of this study is to investigate the influence of shape and size dependant TiO2 nanocrystals/UV laser interaction on the quality of the mass spectra, detectability of analyzed molecules, intensities of the signals and homogeneity of co-crystals onto the MALDI plate, of carbohydrates. It is shown that the size and shape of nanocrystals influenced the way of packing carbohydrates onto plate, and thus affects homogeneity and reproducibility of mass spectrometry analysis.",
publisher = "Springer",
journal = "Optical and Quantum Electronics",
title = "Dependence of the quality of SALDI TOF MS analysis on the TiO2 nanocrystals size and shape",
volume = "48",
number = "2",
doi = "10.1007/s11082-016-0413-5"
}

Popović, I. A., Milovanović, D. S., Miletić, J., Nešić, M. D., Vranješ, M., Šaponjić, Z.,& Petković, M.. (2016). Dependence of the quality of SALDI TOF MS analysis on the TiO2 nanocrystals size and shape. in Optical and Quantum Electronics
Springer., 48(2).
https://doi.org/10.1007/s11082-016-0413-5

Popović IA, Milovanović DS, Miletić J, Nešić MD, Vranješ M, Šaponjić Z, Petković M. Dependence of the quality of SALDI TOF MS analysis on the TiO2 nanocrystals size and shape. in Optical and Quantum Electronics. 2016;48(2).
doi:10.1007/s11082-016-0413-5 .

Popović, Iva A., Milovanović, Dubravka S., Miletić, Jadranka, Nešić, Maja D., Vranješ, Mila, Šaponjić, Zoran, Petković, Marijana, "Dependence of the quality of SALDI TOF MS analysis on the TiO2 nanocrystals size and shape" in Optical and Quantum Electronics, 48, no. 2 (2016),
https://doi.org/10.1007/s11082-016-0413-5 . .

TY  - JOUR
AU  - Nešić, Maja
AU  - Popović, Iva A.
AU  - Leskovac, Andreja
AU  - Šaponjić, Zoran
AU  - Radoičić, Marija B.
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/904
AB  - Photo-responsive drug release systems are promising for drug delivery applications due to many benefits compared to conventional chemotherapy such as targeted, controlled release of a drug and reduced toxicity to healthy tissues. In this work, we report synthesis of the nanocomposite system based on carrier TiO2 nanoparticles and potential anticancer ruthenium complex, with light controllable release properties. Nanocomposite system showed biological activity and induced the generation of free radicals, which are implied in the efficient cell killing. The drug release tests demonstrated sustained release of the transition metal complex from the surface implying the potency for the controlled drug delivery system. Taking into account photoactivity of the Ru-complex, in the next step we have investigated the influence of green light on the rate of the complex release, and the results showed dependence of the Ru-complex release from the surface of TiO2 nanoparticles on the applied laser energy. Therefore, these characteristics make this nanocomposite system promising for the photo-responsive chemotherapy.
PB  - Springer
T2  - Optical and Quantum Electronics
T1  - Testing the photo-sensitive nanocomposite system for potential controlled metallo-drug delivery
VL  - 48
IS  - 2
DO  - 10.1007/s11082-016-0421-5
ER  - 

@article{
author = "Nešić, Maja and Popović, Iva A. and Leskovac, Andreja and Šaponjić, Zoran and Radoičić, Marija B. and Stepić, Milutin and Petković, Marijana",
year = "2016",
abstract = "Photo-responsive drug release systems are promising for drug delivery applications due to many benefits compared to conventional chemotherapy such as targeted, controlled release of a drug and reduced toxicity to healthy tissues. In this work, we report synthesis of the nanocomposite system based on carrier TiO2 nanoparticles and potential anticancer ruthenium complex, with light controllable release properties. Nanocomposite system showed biological activity and induced the generation of free radicals, which are implied in the efficient cell killing. The drug release tests demonstrated sustained release of the transition metal complex from the surface implying the potency for the controlled drug delivery system. Taking into account photoactivity of the Ru-complex, in the next step we have investigated the influence of green light on the rate of the complex release, and the results showed dependence of the Ru-complex release from the surface of TiO2 nanoparticles on the applied laser energy. Therefore, these characteristics make this nanocomposite system promising for the photo-responsive chemotherapy.",
publisher = "Springer",
journal = "Optical and Quantum Electronics",
title = "Testing the photo-sensitive nanocomposite system for potential controlled metallo-drug delivery",
volume = "48",
number = "2",
doi = "10.1007/s11082-016-0421-5"
}

Nešić, M., Popović, I. A., Leskovac, A., Šaponjić, Z., Radoičić, M. B., Stepić, M.,& Petković, M.. (2016). Testing the photo-sensitive nanocomposite system for potential controlled metallo-drug delivery. in Optical and Quantum Electronics
Springer., 48(2).
https://doi.org/10.1007/s11082-016-0421-5

Nešić M, Popović IA, Leskovac A, Šaponjić Z, Radoičić MB, Stepić M, Petković M. Testing the photo-sensitive nanocomposite system for potential controlled metallo-drug delivery. in Optical and Quantum Electronics. 2016;48(2).
doi:10.1007/s11082-016-0421-5 .

Nešić, Maja, Popović, Iva A., Leskovac, Andreja, Šaponjić, Zoran, Radoičić, Marija B., Stepić, Milutin, Petković, Marijana, "Testing the photo-sensitive nanocomposite system for potential controlled metallo-drug delivery" in Optical and Quantum Electronics, 48, no. 2 (2016),
https://doi.org/10.1007/s11082-016-0421-5 . .

TY  - JOUR
AU  - Popović, Iva A.
AU  - Nešić, Maja D.
AU  - Vranješ, Mila
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1267
AB  - Surface-assisted laser desorption/ionisation time-of-flight mass spectrometry (SALDI-TOF-MS) might be the method of choice for the analysis of low mass molecules (less than m/z 500). Titanium dioxide (TiO2) nanocrystals as a substrate for SALDI-TOF-MS improve the reproducibility of the signal intensities and prevent the fragmentation of some molecules upon laser irradiation, as we have previously shown. In addition, variously shaped and sized TiO2 nanocrystals/substrates for SALDI-MS could be used for quantification of small molecules, which are otherwise difficult to detect with the assistance of organic matrices. TiO2-assisted LDI-MS spectra could be acquired with excellent reproducibility and repeatability and with low detection limit. In the current study, we analysed the spectra of dexasone, citric acid, vitamin E and vitamin A acquired with TiO2 nanocrystals of various shapes and dimensions, i.e. the colloidal TiO2 nanoparticles (TiO2 NPs), TiO2 prolate nanospheroids (TiO2 PNSs) and TiO2 nanotubes (TiO2 NTs). Various shapes and dimensions of substrates were used since these factors determine desorption and ionisation processes. The homogeneity on the target plate was compared based on signal-to-noise values of peaks of interest of analysed molecules as well as the within-day and day-to-day repeatability. In summary, the obtained results show that the applicability of individual TiO2 nanocrystals depends on the analyte. Signals which are acquired with the assistance of TiO2 PNSs have the highest sensitivity and reproducibility (the smallest standard deviation), even compared with those in the LDI mode. This implies that TiO2 PNSs could also be suitable for quantitative analyses of small molecules.
T2  - Analytical and Bioanalytical Chemistry
T1  - SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates
VL  - 408
IS  - 26
SP  - 7481
EP  - 7490
DO  - 10.1007/s00216-016-9846-8
ER  - 

@article{
author = "Popović, Iva A. and Nešić, Maja D. and Vranješ, Mila and Šaponjić, Zoran and Petković, Marijana",
year = "2016",
abstract = "Surface-assisted laser desorption/ionisation time-of-flight mass spectrometry (SALDI-TOF-MS) might be the method of choice for the analysis of low mass molecules (less than m/z 500). Titanium dioxide (TiO2) nanocrystals as a substrate for SALDI-TOF-MS improve the reproducibility of the signal intensities and prevent the fragmentation of some molecules upon laser irradiation, as we have previously shown. In addition, variously shaped and sized TiO2 nanocrystals/substrates for SALDI-MS could be used for quantification of small molecules, which are otherwise difficult to detect with the assistance of organic matrices. TiO2-assisted LDI-MS spectra could be acquired with excellent reproducibility and repeatability and with low detection limit. In the current study, we analysed the spectra of dexasone, citric acid, vitamin E and vitamin A acquired with TiO2 nanocrystals of various shapes and dimensions, i.e. the colloidal TiO2 nanoparticles (TiO2 NPs), TiO2 prolate nanospheroids (TiO2 PNSs) and TiO2 nanotubes (TiO2 NTs). Various shapes and dimensions of substrates were used since these factors determine desorption and ionisation processes. The homogeneity on the target plate was compared based on signal-to-noise values of peaks of interest of analysed molecules as well as the within-day and day-to-day repeatability. In summary, the obtained results show that the applicability of individual TiO2 nanocrystals depends on the analyte. Signals which are acquired with the assistance of TiO2 PNSs have the highest sensitivity and reproducibility (the smallest standard deviation), even compared with those in the LDI mode. This implies that TiO2 PNSs could also be suitable for quantitative analyses of small molecules.",
journal = "Analytical and Bioanalytical Chemistry",
title = "SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates",
volume = "408",
number = "26",
pages = "7481-7490",
doi = "10.1007/s00216-016-9846-8"
}

Popović, I. A., Nešić, M. D., Vranješ, M., Šaponjić, Z.,& Petković, M.. (2016). SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates. in Analytical and Bioanalytical Chemistry, 408(26), 7481-7490.
https://doi.org/10.1007/s00216-016-9846-8

Popović IA, Nešić MD, Vranješ M, Šaponjić Z, Petković M. SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates. in Analytical and Bioanalytical Chemistry. 2016;408(26):7481-7490.
doi:10.1007/s00216-016-9846-8 .

Popović, Iva A., Nešić, Maja D., Vranješ, Mila, Šaponjić, Zoran, Petković, Marijana, "SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates" in Analytical and Bioanalytical Chemistry, 408, no. 26 (2016):7481-7490,
https://doi.org/10.1007/s00216-016-9846-8 . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Popović, Iva A.
AU  - Leskovac, Andreja
AU  - Petković, Marijana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1271
AB  - Ruthenium compounds are highly regarded as metallo-drug candidates. Many studies have focused their attention on the interaction between ruthenium complexes with their possible biological targets. The interaction of ruthenium complexes with transport proteins, enzymes and peptides is of great importance for understanding their biodistribution and mechanism of action, therefore, the development of an anti-cancer therapy involving ruthenium complexes has recently shifted from DNA targeting towards protein targeting. With the aim of gaining insight into possible interactions between ruthenium complexes with biologically relevant proteins, we have studied the interaction of cis-dichlorobis(2,2-bipyridyl-4,4-dicarboxylic acid)ruthenium(II) complex [Ru(II)(dcbpy)(2)Cl-2], which previously showed good potency in photo-dynamic chemotherapy, with bovine serum albumin (BSA), phospholipase A(2) (PLA(2)) and glutathione (GSH). Binding constants and possible number of binding sites to mentioned proteins and peptide are investigated by ultraviolet-visible spectroscopy and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI TOF MS). The complex binding affinities were in the following order: PLA(2) GT BSA GT GSH. Moreover, genotoxic profile of the complex, tested on peripheral blood lymphocytes as a model system, was also promising.
T2  - Biometals
T1  - Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione
VL  - 29
IS  - 5
SP  - 921
EP  - 933
DO  - 10.1007/s10534-016-9964-y
ER  - 

@article{
author = "Nešić, Maja D. and Popović, Iva A. and Leskovac, Andreja and Petković, Marijana",
year = "2016",
abstract = "Ruthenium compounds are highly regarded as metallo-drug candidates. Many studies have focused their attention on the interaction between ruthenium complexes with their possible biological targets. The interaction of ruthenium complexes with transport proteins, enzymes and peptides is of great importance for understanding their biodistribution and mechanism of action, therefore, the development of an anti-cancer therapy involving ruthenium complexes has recently shifted from DNA targeting towards protein targeting. With the aim of gaining insight into possible interactions between ruthenium complexes with biologically relevant proteins, we have studied the interaction of cis-dichlorobis(2,2-bipyridyl-4,4-dicarboxylic acid)ruthenium(II) complex [Ru(II)(dcbpy)(2)Cl-2], which previously showed good potency in photo-dynamic chemotherapy, with bovine serum albumin (BSA), phospholipase A(2) (PLA(2)) and glutathione (GSH). Binding constants and possible number of binding sites to mentioned proteins and peptide are investigated by ultraviolet-visible spectroscopy and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI TOF MS). The complex binding affinities were in the following order: PLA(2) GT BSA GT GSH. Moreover, genotoxic profile of the complex, tested on peripheral blood lymphocytes as a model system, was also promising.",
journal = "Biometals",
title = "Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione",
volume = "29",
number = "5",
pages = "921-933",
doi = "10.1007/s10534-016-9964-y"
}

Nešić, M. D., Popović, I. A., Leskovac, A.,& Petković, M.. (2016). Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione. in Biometals, 29(5), 921-933.
https://doi.org/10.1007/s10534-016-9964-y

Nešić MD, Popović IA, Leskovac A, Petković M. Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione. in Biometals. 2016;29(5):921-933.
doi:10.1007/s10534-016-9964-y .

Nešić, Maja D., Popović, Iva A., Leskovac, Andreja, Petković, Marijana, "Biological activity and binding properties of [Ru(II)(dcbpy)(2)Cl-2] complex to bovine serum albumin, phospholipase A(2) and glutathione" in Biometals, 29, no. 5 (2016):921-933,
https://doi.org/10.1007/s10534-016-9964-y . .

TY  - JOUR
AU  - Jovanović, Snežana
AU  - Obrencevic, Katarina
AU  - Bugarčić, Živadin D.
AU  - Popović, Iva A.
AU  - Žakula, Jelena
AU  - Petrović, Biljana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1208
AB  - Two new dinuclear bimetallic complexes, [{PdCl(bipy)}{mu-(pyrazine)}{PtCl(bipy)}]Cl(ClO4) (1) (bipy is 2,2-=bipyridine) and [{PdCl(en)}{mu-(pyrazine)}{PtCl(en)}]Cl(ClO4) (2) (en is ethylenediamine), have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR spectroscopy and MALDI-TOF mass spectrometry. The pK(a) values of the coordinated water molecules of the diaqua species were determined as well. Substitution reactions of complexes (1) and (2) with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP) were studied under the pseudo-first order conditions as a function of nucleophile concentration and temperature. The order of reactivity of nucleophiles was: Tu GT L-Met GT L-Cys GT L-His GT 5-GMP. Substitution reactions with Tu, L-Cys and L-His were followed by decomposition of bimetallic complexes to the corresponding substituted mononuclear complexes [Pd(N-N)(Nu)(2)] and [Pt(N-N)(Nu)(2)] (N-N = bipy, en), releasing the bridging ligand. However, the structures of starting bimetallic complexes were preserved during the reactions with L-Met and 5-GMP. The absorption spectroscopic study of interactions of calf-thymus DNA (CT-DNA) with complexes (1), (2) and [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)} {PtCl(bipy)}] Cl(ClO4) (3), has shown that all the complexes exhibit high intrinsic binding constants (K-b = 10(4)-10(5) M-1). DNA-ethidium bromide (DNA-EB) fluorescence was quenched after addition of complexes (1), (2) or (3), indicating displacement of intercalating EB by complexes. All complexes have shown good binding affinity to bovine serum albumin protein (BSA). Chemosensitivity of A375 (human melanoma) and HeLa (human cervical cancer) cell lines toward complexes (1), (2) and (3) was analyzed by SRB assay. Complex (1) displayed significant inhibitory effect on the growth of both cell lines.
T2  - Dalton Transactions
T1  - New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity
VL  - 45
IS  - 31
SP  - 12444
EP  - 12457
DO  - 10.1039/c6dt02226j
ER  - 

@article{
author = "Jovanović, Snežana and Obrencevic, Katarina and Bugarčić, Živadin D. and Popović, Iva A. and Žakula, Jelena and Petrović, Biljana",
year = "2016",
abstract = "Two new dinuclear bimetallic complexes, [{PdCl(bipy)}{mu-(pyrazine)}{PtCl(bipy)}]Cl(ClO4) (1) (bipy is 2,2-=bipyridine) and [{PdCl(en)}{mu-(pyrazine)}{PtCl(en)}]Cl(ClO4) (2) (en is ethylenediamine), have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR spectroscopy and MALDI-TOF mass spectrometry. The pK(a) values of the coordinated water molecules of the diaqua species were determined as well. Substitution reactions of complexes (1) and (2) with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP) were studied under the pseudo-first order conditions as a function of nucleophile concentration and temperature. The order of reactivity of nucleophiles was: Tu GT L-Met GT L-Cys GT L-His GT 5-GMP. Substitution reactions with Tu, L-Cys and L-His were followed by decomposition of bimetallic complexes to the corresponding substituted mononuclear complexes [Pd(N-N)(Nu)(2)] and [Pt(N-N)(Nu)(2)] (N-N = bipy, en), releasing the bridging ligand. However, the structures of starting bimetallic complexes were preserved during the reactions with L-Met and 5-GMP. The absorption spectroscopic study of interactions of calf-thymus DNA (CT-DNA) with complexes (1), (2) and [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)} {PtCl(bipy)}] Cl(ClO4) (3), has shown that all the complexes exhibit high intrinsic binding constants (K-b = 10(4)-10(5) M-1). DNA-ethidium bromide (DNA-EB) fluorescence was quenched after addition of complexes (1), (2) or (3), indicating displacement of intercalating EB by complexes. All complexes have shown good binding affinity to bovine serum albumin protein (BSA). Chemosensitivity of A375 (human melanoma) and HeLa (human cervical cancer) cell lines toward complexes (1), (2) and (3) was analyzed by SRB assay. Complex (1) displayed significant inhibitory effect on the growth of both cell lines.",
journal = "Dalton Transactions",
title = "New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity",
volume = "45",
number = "31",
pages = "12444-12457",
doi = "10.1039/c6dt02226j"
}

Jovanović, S., Obrencevic, K., Bugarčić, Ž. D., Popović, I. A., Žakula, J.,& Petrović, B.. (2016). New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity. in Dalton Transactions, 45(31), 12444-12457.
https://doi.org/10.1039/c6dt02226j

Jovanović S, Obrencevic K, Bugarčić ŽD, Popović IA, Žakula J, Petrović B. New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity. in Dalton Transactions. 2016;45(31):12444-12457.
doi:10.1039/c6dt02226j .

Jovanović, Snežana, Obrencevic, Katarina, Bugarčić, Živadin D., Popović, Iva A., Žakula, Jelena, Petrović, Biljana, "New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity" in Dalton Transactions, 45, no. 31 (2016):12444-12457,
https://doi.org/10.1039/c6dt02226j . .

TY  - CONF
AU  - Popović, Iva A.
AU  - Nešić, Mioljub V.
AU  - Nišavić, Marija
AU  - Petković, Marijana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7113
C3  - FEBS Journal
T1  - Testing the best matrix/analyte combination for MALDI TOF mass spectrometric detection of steroid hormones, amino acids, vitamins and carbohydrates
VL  - 283
SP  - 166
EP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7113
ER  - 

@conference{
author = "Popović, Iva A. and Nešić, Mioljub V. and Nišavić, Marija and Petković, Marijana",
year = "2016",
journal = "FEBS Journal",
title = "Testing the best matrix/analyte combination for MALDI TOF mass spectrometric detection of steroid hormones, amino acids, vitamins and carbohydrates",
volume = "283",
pages = "166-166",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7113"
}

Popović, I. A., Nešić, M. V., Nišavić, M.,& Petković, M.. (2016). Testing the best matrix/analyte combination for MALDI TOF mass spectrometric detection of steroid hormones, amino acids, vitamins and carbohydrates. in FEBS Journal, 283, 166-166.
https://hdl.handle.net/21.15107/rcub_vinar_7113

Popović IA, Nešić MV, Nišavić M, Petković M. Testing the best matrix/analyte combination for MALDI TOF mass spectrometric detection of steroid hormones, amino acids, vitamins and carbohydrates. in FEBS Journal. 2016;283:166-166.
https://hdl.handle.net/21.15107/rcub_vinar_7113 .

Popović, Iva A., Nešić, Mioljub V., Nišavić, Marija, Petković, Marijana, "Testing the best matrix/analyte combination for MALDI TOF mass spectrometric detection of steroid hormones, amino acids, vitamins and carbohydrates" in FEBS Journal, 283 (2016):166-166,
https://hdl.handle.net/21.15107/rcub_vinar_7113 .

TY  - JOUR
AU  - Popović, Iva A.
AU  - Nešić, Maja D.
AU  - Nišavić, Marija
AU  - Vranješ, Mila
AU  - Radetić, Tamara
AU  - Šaponjić, Zoran
AU  - Masnikosa, Romana
AU  - Petković, Marijana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/544
AB  - Nanostructured materials as substrates for surface-assisted laser desorption and ionization time-of-flight mass spectrometry have two main advantages such as small number of signals in the low mass range and salt tolerance. We have also previously observed decreased extent of undesired fragmentation of bipyridine-containing transition metal complexes in the presence of TiO2 nanoparticles (NPs). In this work, we extended our previous study and tested whether both TiO2 NPs and TiO2 prolate nanospheroids (PNSs) can be used as substrates for SALDI-MS of bipyridine-containing transition metal complexes and whether they tolerate intense laser and the presence of inorganic salts. TiO2 NPs (an average diameter of 5 nm) and PNSs (length: 40-50 nm, the lateral dimension: 14-16 nm) were characterized microscopically and their mass spectra were analyzed, as well as the spectra of bipyridine-containing Pt(II) and Ru(III) complexes. Our results show that seven times higher analyte signals can be obtained in the presence of PNSs compared to the signals acquired with NPs and three times higher compared to spectra acquired without a substrate. The presence of inorganic salt did not affect the spectra, which makes both TiO2 nanocrystals suitable for MS analysis of transition metal complexes in biological fluids. (C) 2015 Elsevier B.V. All rights reserved.
T2  - Materials Letters
T1  - Suitability of TiO2 nanoparticles and prolate nanospheroids for laser desorption/ionization mass spectrometric characterization of bipyridine-containing complexes
VL  - 150
SP  - 84
EP  - 88
DO  - 10.1016/j.matlet.2015.03.004
ER  - 

@article{
author = "Popović, Iva A. and Nešić, Maja D. and Nišavić, Marija and Vranješ, Mila and Radetić, Tamara and Šaponjić, Zoran and Masnikosa, Romana and Petković, Marijana",
year = "2015",
abstract = "Nanostructured materials as substrates for surface-assisted laser desorption and ionization time-of-flight mass spectrometry have two main advantages such as small number of signals in the low mass range and salt tolerance. We have also previously observed decreased extent of undesired fragmentation of bipyridine-containing transition metal complexes in the presence of TiO2 nanoparticles (NPs). In this work, we extended our previous study and tested whether both TiO2 NPs and TiO2 prolate nanospheroids (PNSs) can be used as substrates for SALDI-MS of bipyridine-containing transition metal complexes and whether they tolerate intense laser and the presence of inorganic salts. TiO2 NPs (an average diameter of 5 nm) and PNSs (length: 40-50 nm, the lateral dimension: 14-16 nm) were characterized microscopically and their mass spectra were analyzed, as well as the spectra of bipyridine-containing Pt(II) and Ru(III) complexes. Our results show that seven times higher analyte signals can be obtained in the presence of PNSs compared to the signals acquired with NPs and three times higher compared to spectra acquired without a substrate. The presence of inorganic salt did not affect the spectra, which makes both TiO2 nanocrystals suitable for MS analysis of transition metal complexes in biological fluids. (C) 2015 Elsevier B.V. All rights reserved.",
journal = "Materials Letters",
title = "Suitability of TiO2 nanoparticles and prolate nanospheroids for laser desorption/ionization mass spectrometric characterization of bipyridine-containing complexes",
volume = "150",
pages = "84-88",
doi = "10.1016/j.matlet.2015.03.004"
}

Popović, I. A., Nešić, M. D., Nišavić, M., Vranješ, M., Radetić, T., Šaponjić, Z., Masnikosa, R.,& Petković, M.. (2015). Suitability of TiO2 nanoparticles and prolate nanospheroids for laser desorption/ionization mass spectrometric characterization of bipyridine-containing complexes. in Materials Letters, 150, 84-88.
https://doi.org/10.1016/j.matlet.2015.03.004

Popović IA, Nešić MD, Nišavić M, Vranješ M, Radetić T, Šaponjić Z, Masnikosa R, Petković M. Suitability of TiO2 nanoparticles and prolate nanospheroids for laser desorption/ionization mass spectrometric characterization of bipyridine-containing complexes. in Materials Letters. 2015;150:84-88.
doi:10.1016/j.matlet.2015.03.004 .

Popović, Iva A., Nešić, Maja D., Nišavić, Marija, Vranješ, Mila, Radetić, Tamara, Šaponjić, Zoran, Masnikosa, Romana, Petković, Marijana, "Suitability of TiO2 nanoparticles and prolate nanospheroids for laser desorption/ionization mass spectrometric characterization of bipyridine-containing complexes" in Materials Letters, 150 (2015):84-88,
https://doi.org/10.1016/j.matlet.2015.03.004 . .

TY  - CONF
AU  - Popović, Iva
AU  - Nešić, Maja
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12008
AB  - The important factor that determines the process of the laser-induced desorption and ionization of molecules for the mass spectrometric analysis is the interaction between the laser light and the substrate. It is of importance that substrate efficiently absorbs laser energy, which will be further rapidly transferred to the analyte. The overall goal in this process is to obtain good quality mass spectrum with low degree of fragmentation. Whereas the application of organic matrices instead of substrates results in the numerous undesired polymerization reactions, which complicate the spectra, and in some cases, even prevent the detection of the ions of interests, nanoparticles as substrates are convenient due to a lower number of background signals and low onset of fragmentation reactions in the gas phase. Titanium dioxide (TiO2) is semiconductor-based and widely used substrate for laser desorption and ionization ((SA)LDI), and when it is in the excited state it transfers an electron or energy into the ground state molecule. This process is called sensitized photoreaction [1]. The desorption/ionization processes on TiO2 nanoparticles are related to the physical properties of the substrate such as ability to absorb and dissipate energy from the irradiating laser light source [2]. TiO2 has a large band gap (bulk anatase: 3.2 eV), and can therefore be used as a SALDI matrix with the N2 laser (337 nm) [3]. The aim of our study is to investigate the influence, which the interaction of the UV laser with TiO2 nanoparticles of various shapes and size has on the quality of the mass spectra of carbohydrates: D-(+)-glucose, D-(+)-maltose, raffinose, arabinose, β-ciclodextrine, substances which are otherwise difficult for mass spectrometric analysis. For this purpose, we used small, nearly spherically shaped colloidal TiO2 nanoparticles (average diameter ~ 5 nm), prolate nanospheroids (length: 40–50 nm, the lateral dimension: 14–16 nm) and nanotubes (length: 100-150 nm, average diameter 11 nm). For comparison, the spectra are acquired also with traditionally used organic matrices. The spectra of carbohydrates with organic matrices are overloaded with matrix signals, in which case the signals arising from the analyte of interest are suppressed, or it is possible to detect only adducts with matrix. Laser-induced ionization on nanostructures offers alternative ionization pathways through the formation of Na+ and K+ adducts with appreciable yield [4]. TiO2 nanotubes showed extraordinary properties for detection of carbohydrates. Arabinose was detectable only with TiO2, and D-(+)-glucose and D-(+)-maltose were detected in negative ion mode, which was not the case with other organic matrices and substrates. Not only the highest intensities of mass peaks but also the smallest coefficient of variation was achieved with TiO2 nanotubes. Taken toghether, TiO2 nanotubes, due to their size and the shape, have the most suitable physical properties for the substrate in the SALDI technique. The laser intensity was kept as low as possible in order to prevent fragmentaion. Higher laser intensity is required for the process of desorption/ionization when TiO2 NPs are used (2400 i.u.), but for TiO2 PNSs and TiO2 nanotubes were lower and nearly the same 1950 and 2000 i.u., respectively. In conclusion, larger, tube-shaped TiO2 substrates more efficiently absorb the laser energy and transfer it to the carbohydrates, enabling their desorption/ionization and preventing their fragmentation.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2015 : 5th International School and Conference on Photonics and COST actions: MP1204, BM1205 and MP1205 : book of abstracts; August 24-28, 2015; Belgrade
T1  - The interaction between variously shaped TiO2 nanoparticles with UV laser determines the quality of the mass spectra of carbohydrates
SP  - 101
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12008
ER  - 

@conference{
author = "Popović, Iva and Nešić, Maja and Šaponjić, Zoran and Petković, Marijana",
year = "2015",
abstract = "The important factor that determines the process of the laser-induced desorption and ionization of molecules for the mass spectrometric analysis is the interaction between the laser light and the substrate. It is of importance that substrate efficiently absorbs laser energy, which will be further rapidly transferred to the analyte. The overall goal in this process is to obtain good quality mass spectrum with low degree of fragmentation. Whereas the application of organic matrices instead of substrates results in the numerous undesired polymerization reactions, which complicate the spectra, and in some cases, even prevent the detection of the ions of interests, nanoparticles as substrates are convenient due to a lower number of background signals and low onset of fragmentation reactions in the gas phase. Titanium dioxide (TiO2) is semiconductor-based and widely used substrate for laser desorption and ionization ((SA)LDI), and when it is in the excited state it transfers an electron or energy into the ground state molecule. This process is called sensitized photoreaction [1]. The desorption/ionization processes on TiO2 nanoparticles are related to the physical properties of the substrate such as ability to absorb and dissipate energy from the irradiating laser light source [2]. TiO2 has a large band gap (bulk anatase: 3.2 eV), and can therefore be used as a SALDI matrix with the N2 laser (337 nm) [3]. The aim of our study is to investigate the influence, which the interaction of the UV laser with TiO2 nanoparticles of various shapes and size has on the quality of the mass spectra of carbohydrates: D-(+)-glucose, D-(+)-maltose, raffinose, arabinose, β-ciclodextrine, substances which are otherwise difficult for mass spectrometric analysis. For this purpose, we used small, nearly spherically shaped colloidal TiO2 nanoparticles (average diameter ~ 5 nm), prolate nanospheroids (length: 40–50 nm, the lateral dimension: 14–16 nm) and nanotubes (length: 100-150 nm, average diameter 11 nm). For comparison, the spectra are acquired also with traditionally used organic matrices. The spectra of carbohydrates with organic matrices are overloaded with matrix signals, in which case the signals arising from the analyte of interest are suppressed, or it is possible to detect only adducts with matrix. Laser-induced ionization on nanostructures offers alternative ionization pathways through the formation of Na+ and K+ adducts with appreciable yield [4]. TiO2 nanotubes showed extraordinary properties for detection of carbohydrates. Arabinose was detectable only with TiO2, and D-(+)-glucose and D-(+)-maltose were detected in negative ion mode, which was not the case with other organic matrices and substrates. Not only the highest intensities of mass peaks but also the smallest coefficient of variation was achieved with TiO2 nanotubes. Taken toghether, TiO2 nanotubes, due to their size and the shape, have the most suitable physical properties for the substrate in the SALDI technique. The laser intensity was kept as low as possible in order to prevent fragmentaion. Higher laser intensity is required for the process of desorption/ionization when TiO2 NPs are used (2400 i.u.), but for TiO2 PNSs and TiO2 nanotubes were lower and nearly the same 1950 and 2000 i.u., respectively. In conclusion, larger, tube-shaped TiO2 substrates more efficiently absorb the laser energy and transfer it to the carbohydrates, enabling their desorption/ionization and preventing their fragmentation.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2015 : 5th International School and Conference on Photonics and COST actions: MP1204, BM1205 and MP1205 : book of abstracts; August 24-28, 2015; Belgrade",
title = "The interaction between variously shaped TiO2 nanoparticles with UV laser determines the quality of the mass spectra of carbohydrates",
pages = "101-102",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12008"
}

Popović, I., Nešić, M., Šaponjić, Z.,& Petković, M.. (2015). The interaction between variously shaped TiO2 nanoparticles with UV laser determines the quality of the mass spectra of carbohydrates. in PHOTONICA2015 : 5th International School and Conference on Photonics and COST actions: MP1204, BM1205 and MP1205 : book of abstracts; August 24-28, 2015; Belgrade
Belgrade : Vinča Institute of Nuclear Sciences., 101-102.
https://hdl.handle.net/21.15107/rcub_vinar_12008

Popović I, Nešić M, Šaponjić Z, Petković M. The interaction between variously shaped TiO2 nanoparticles with UV laser determines the quality of the mass spectra of carbohydrates. in PHOTONICA2015 : 5th International School and Conference on Photonics and COST actions: MP1204, BM1205 and MP1205 : book of abstracts; August 24-28, 2015; Belgrade. 2015;:101-102.
https://hdl.handle.net/21.15107/rcub_vinar_12008 .

Popović, Iva, Nešić, Maja, Šaponjić, Zoran, Petković, Marijana, "The interaction between variously shaped TiO2 nanoparticles with UV laser determines the quality of the mass spectra of carbohydrates" in PHOTONICA2015 : 5th International School and Conference on Photonics and COST actions: MP1204, BM1205 and MP1205 : book of abstracts; August 24-28, 2015; Belgrade (2015):101-102,
https://hdl.handle.net/21.15107/rcub_vinar_12008 .

TY  - JOUR
AU  - Radisavljevic, Maja
AU  - Kamceva, Tina
AU  - Vukićević, Iva A.
AU  - Nišavić, Marija
AU  - Milovanović, Milan
AU  - Petković, Marijana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5605
AB  - We compare the quality and reliability of laser desorption and ionisation mass spectra of FeCl3 acquired without the assistance of the matrix with the spectra acquired with different organic matrix molecules. Generally, inorganic salts tend to form clusters upon laser irradiation, the signals of which can be easily distinguished from ions arising from the matrix. In the presence of a matrix, cluster ions are, however, mostly suppressed. We have compared the number of analyte signals, accuracy of determination of isotope composition of the analyte and the sensitivity of FeCl3 detection between different approaches. The results obtained imply that the sensitivity of mass spectrometric analysis of FeCl3 is somewhat higher when matrices are applied than in the matrix-free approach. Among all matrices tested in this work, F20TPP seems to be the most promising for further applications as a matrix for mass spectrometry of inorganic salts.
T2  - European Journal of Mass Spectrometry
T1  - Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach
VL  - 19
IS  - 2
SP  - 77
EP  - 89
DO  - 10.1255/ejms.1217
ER  - 

@article{
author = "Radisavljevic, Maja and Kamceva, Tina and Vukićević, Iva A. and Nišavić, Marija and Milovanović, Milan and Petković, Marijana",
year = "2013",
abstract = "We compare the quality and reliability of laser desorption and ionisation mass spectra of FeCl3 acquired without the assistance of the matrix with the spectra acquired with different organic matrix molecules. Generally, inorganic salts tend to form clusters upon laser irradiation, the signals of which can be easily distinguished from ions arising from the matrix. In the presence of a matrix, cluster ions are, however, mostly suppressed. We have compared the number of analyte signals, accuracy of determination of isotope composition of the analyte and the sensitivity of FeCl3 detection between different approaches. The results obtained imply that the sensitivity of mass spectrometric analysis of FeCl3 is somewhat higher when matrices are applied than in the matrix-free approach. Among all matrices tested in this work, F20TPP seems to be the most promising for further applications as a matrix for mass spectrometry of inorganic salts.",
journal = "European Journal of Mass Spectrometry",
title = "Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach",
volume = "19",
number = "2",
pages = "77-89",
doi = "10.1255/ejms.1217"
}

Radisavljevic, M., Kamceva, T., Vukićević, I. A., Nišavić, M., Milovanović, M.,& Petković, M.. (2013). Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach. in European Journal of Mass Spectrometry, 19(2), 77-89.
https://doi.org/10.1255/ejms.1217

Radisavljevic M, Kamceva T, Vukićević IA, Nišavić M, Milovanović M, Petković M. Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach. in European Journal of Mass Spectrometry. 2013;19(2):77-89.
doi:10.1255/ejms.1217 .

Radisavljevic, Maja, Kamceva, Tina, Vukićević, Iva A., Nišavić, Marija, Milovanović, Milan, Petković, Marijana, "Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach" in European Journal of Mass Spectrometry, 19, no. 2 (2013):77-89,
https://doi.org/10.1255/ejms.1217 . .

TY  - JOUR
AU  - Kamceva, Tina
AU  - Radisavljević, Maja
AU  - Vukićević, Iva A.
AU  - Arnhold, Juergen
AU  - Petković, Marijana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5790
AB  - Phospholipase A(2) is involved in propagation of inflammatory processes and carcinogenesis through its role in phospholipid metabolism, and release of arachidonic acid and lysophospholipids. Recent findings on correlation between elevated PLA(2) activity and metastatic cancer render this enzyme an attractive target for cancer therapy. On the other hand, due to a broad range of oxidation states under physiological conditions and a high affinity for protein binding, platinum and ruthenium coordination complexes are promising candidates for PLA(2) inhibitors. In this article, we discuss the interactions of Pt and Ru coordination complexes with PLA(2) and phospholipids, as well as the application of MALDI-TOF mass spectrometry for screening PLA(2) inhibitors. Owing to the ability of this technique to simultaneously detect and monitor changes in substrate and product concentrations, the inhibitor mechanisms of both Pt and Ru complexes with various ligands were determined.
T2  - Chemistry and Biodiversity
T1  - Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry
VL  - 10
IS  - 11
SP  - 1972
EP  - 1986
DO  - 10.1002/cbdv.201300141
ER  - 

@article{
author = "Kamceva, Tina and Radisavljević, Maja and Vukićević, Iva A. and Arnhold, Juergen and Petković, Marijana",
year = "2013",
abstract = "Phospholipase A(2) is involved in propagation of inflammatory processes and carcinogenesis through its role in phospholipid metabolism, and release of arachidonic acid and lysophospholipids. Recent findings on correlation between elevated PLA(2) activity and metastatic cancer render this enzyme an attractive target for cancer therapy. On the other hand, due to a broad range of oxidation states under physiological conditions and a high affinity for protein binding, platinum and ruthenium coordination complexes are promising candidates for PLA(2) inhibitors. In this article, we discuss the interactions of Pt and Ru coordination complexes with PLA(2) and phospholipids, as well as the application of MALDI-TOF mass spectrometry for screening PLA(2) inhibitors. Owing to the ability of this technique to simultaneously detect and monitor changes in substrate and product concentrations, the inhibitor mechanisms of both Pt and Ru complexes with various ligands were determined.",
journal = "Chemistry and Biodiversity",
title = "Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry",
volume = "10",
number = "11",
pages = "1972-1986",
doi = "10.1002/cbdv.201300141"
}

Kamceva, T., Radisavljević, M., Vukićević, I. A., Arnhold, J.,& Petković, M.. (2013). Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry. in Chemistry and Biodiversity, 10(11), 1972-1986.
https://doi.org/10.1002/cbdv.201300141

Kamceva T, Radisavljević M, Vukićević IA, Arnhold J, Petković M. Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry. in Chemistry and Biodiversity. 2013;10(11):1972-1986.
doi:10.1002/cbdv.201300141 .

Kamceva, Tina, Radisavljević, Maja, Vukićević, Iva A., Arnhold, Juergen, Petković, Marijana, "Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry" in Chemistry and Biodiversity, 10, no. 11 (2013):1972-1986,
https://doi.org/10.1002/cbdv.201300141 . .

TY  - JOUR
AU  - Radisavljević, Maja
AU  - Kamceva, Tina
AU  - Vukićević, Iva A.
AU  - Radoičić, Marija B.
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4972
AB  - RATIONALE Nanoparticles as substrates for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) have advantages over organic matrices, since they enable acquisition of spectra in the low-mass range. It has been previously shown that TiO2 nanoparticles can be used as substrate for MALDI-TOF MS analysis of phospholipids and for other types of molecules, but none of them was applied to the analysis of transition metal complexes. METHODS The MALDI-TOF mass spectra of potential anti-tumor drugs [AuCl2(bipy)]Cl, [PtCl4(bipy)], and [RuCl2(bipy)2]Cl acquired with organic matrices have been compared with spectra acquired with colloidal titanium dioxide nanoparticles. Colloidal TiO2 nanoparticles (NPs) with average diameter of 5 nm were synthesized and characterized by microscopy. For some experiments, the TiO2 NPs were treated at 60 degrees C. Suspensions of matrix and the analyte were premixed, applied to the MALDI target and left at room temperature. Mass spectra were acquired with a 50-Hz pulsed nitrogen laser emitting at a wavelength of 337 nm. RESULTS The MALDI spectra of transition metal complexes acquired with TiO2 NPs exhibited somewhat lower sensitivity than those with organic matrices; on the other hand, they are characterized by significantly lower number of signals arising from the tested complexes than the organic matrices. Whereas adducts between organic matrices and the analytes were detectable in the spectra, this was not the case for the TiO2-assisted mass spectra. CONCLUSIONS We have shown that colloidal TiO2 NPs can be used as substrates for MALDI-TOF MS of transition metal complexes. Although the sensitivity of this approach in comparison with the use of organic matrices might still be a problem, the potential of the applications of NPs for the mass spectrometric characterization of transition metal complexes is clearly demonstrated. Copyright (C) 2012 John Wiley and Sons, Ltd.
T2  - Rapid Communications in Mass Spectrometry
T1  - Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes
VL  - 26
IS  - 17
SP  - 2041
EP  - 2050
DO  - 10.1002/rcm.6320
ER  - 

@article{
author = "Radisavljević, Maja and Kamceva, Tina and Vukićević, Iva A. and Radoičić, Marija B. and Šaponjić, Zoran and Petković, Marijana",
year = "2012",
abstract = "RATIONALE Nanoparticles as substrates for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) have advantages over organic matrices, since they enable acquisition of spectra in the low-mass range. It has been previously shown that TiO2 nanoparticles can be used as substrate for MALDI-TOF MS analysis of phospholipids and for other types of molecules, but none of them was applied to the analysis of transition metal complexes. METHODS The MALDI-TOF mass spectra of potential anti-tumor drugs [AuCl2(bipy)]Cl, [PtCl4(bipy)], and [RuCl2(bipy)2]Cl acquired with organic matrices have been compared with spectra acquired with colloidal titanium dioxide nanoparticles. Colloidal TiO2 nanoparticles (NPs) with average diameter of 5 nm were synthesized and characterized by microscopy. For some experiments, the TiO2 NPs were treated at 60 degrees C. Suspensions of matrix and the analyte were premixed, applied to the MALDI target and left at room temperature. Mass spectra were acquired with a 50-Hz pulsed nitrogen laser emitting at a wavelength of 337 nm. RESULTS The MALDI spectra of transition metal complexes acquired with TiO2 NPs exhibited somewhat lower sensitivity than those with organic matrices; on the other hand, they are characterized by significantly lower number of signals arising from the tested complexes than the organic matrices. Whereas adducts between organic matrices and the analytes were detectable in the spectra, this was not the case for the TiO2-assisted mass spectra. CONCLUSIONS We have shown that colloidal TiO2 NPs can be used as substrates for MALDI-TOF MS of transition metal complexes. Although the sensitivity of this approach in comparison with the use of organic matrices might still be a problem, the potential of the applications of NPs for the mass spectrometric characterization of transition metal complexes is clearly demonstrated. Copyright (C) 2012 John Wiley and Sons, Ltd.",
journal = "Rapid Communications in Mass Spectrometry",
title = "Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes",
volume = "26",
number = "17",
pages = "2041-2050",
doi = "10.1002/rcm.6320"
}

Radisavljević, M., Kamceva, T., Vukićević, I. A., Radoičić, M. B., Šaponjić, Z.,& Petković, M.. (2012). Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes. in Rapid Communications in Mass Spectrometry, 26(17), 2041-2050.
https://doi.org/10.1002/rcm.6320

Radisavljević M, Kamceva T, Vukićević IA, Radoičić MB, Šaponjić Z, Petković M. Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes. in Rapid Communications in Mass Spectrometry. 2012;26(17):2041-2050.
doi:10.1002/rcm.6320 .

Radisavljević, Maja, Kamceva, Tina, Vukićević, Iva A., Radoičić, Marija B., Šaponjić, Zoran, Petković, Marijana, "Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes" in Rapid Communications in Mass Spectrometry, 26, no. 17 (2012):2041-2050,
https://doi.org/10.1002/rcm.6320 . .