Popović, Nataša M.

Link to this page

Authority KeyName Variants
orcid::0000-0002-7749-8375
  • Popović, Nataša M. (27)

Author's Bibliography

The effect of antioxidant status on overall survival in renal cell carcinoma

Pavlović, Ivan; Pejić, Snežana; Radojević-Škodrić, Sanja; Todorović, Ana; Stojiljković, Vesna; Gavrilović, Ljubica; Popović, Nataša M.; Basta-Jovanović, Gordana; Džamić, Zoran; Pajović, Snežana B.

(2020)


                                            

                                            
Pavlović, I., Pejić, S., Radojević-Škodrić, S., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M., Basta-Jovanović, G., Džamić, Z.,& Pajović, S. B. (2020). The effect of antioxidant status on overall survival in renal cell carcinoma.
Archives of Medical Science, 16(1), 94-101.
https://doi.org/10.5114/aoms.2019.86818
Pavlović I, Pejić S, Radojević-Škodrić S, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Basta-Jovanović G, Džamić Z, Pajović SB. The effect of antioxidant status on overall survival in renal cell carcinoma. Archives of Medical Science. 2020;16(1):94-101
Pavlović Ivan, Pejić Snežana, Radojević-Škodrić Sanja, Todorović Ana, Stojiljković Vesna, Gavrilović Ljubica, Popović Nataša M., Basta-Jovanović Gordana, Džamić Zoran, Pajović Snežana B., "The effect of antioxidant status on overall survival in renal cell carcinoma" Archives of Medical Science, 16, no. 1 (2020):94-101,
https://doi.org/10.5114/aoms.2019.86818 .
1
1
1
1

Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium

Popović, Nataša M.; Stojiljković, Vesna; Pejić, Snežana; Todorović, Ana; Pavlović, Ivan; Gavrilović, Ljubica; Pajović, Snežana B.

(2019)

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
AU  - Pajović, Snežana B.
PY  - 2019
UR  - https://www.hindawi.com/journals/omcl/2019/8745376/
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8085
AB  - This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium
VL  - 2019
SP  - 1
EP  - 11
DO  - 10.1155/2019/8745376
ER  - 
@article{
author = "Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Gavrilović, Ljubica and Pajović, Snežana B.",
year = "2019",
url = "https://www.hindawi.com/journals/omcl/2019/8745376/, http://vinar.vin.bg.ac.rs/handle/123456789/8085",
abstract = "This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium",
volume = "2019",
pages = "1-11",
doi = "10.1155/2019/8745376"
}
Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I., Gavrilović, L.,& Pajović, S. B. (2019). Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium.
Oxidative Medicine and Cellular Longevity, 2019, 1-11.
https://doi.org/10.1155/2019/8745376
Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Gavrilović L, Pajović SB. Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium. Oxidative Medicine and Cellular Longevity. 2019;2019:1-11
Popović Nataša M., Stojiljković Vesna, Pejić Snežana, Todorović Ana, Pavlović Ivan, Gavrilović Ljubica, Pajović Snežana B., "Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium" Oxidative Medicine and Cellular Longevity, 2019 (2019):1-11,
https://doi.org/10.1155/2019/8745376 .
4
2
3

Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma

Todorović, Ana; Pejić, Snežana; Gavrilović, Ljubica; Pavlović, Ivan; Stojiljković, Vesna; Popović, Nataša M.; Pajović, Snežana B.

(2019)

TY  - JOUR
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Gavrilović, Ljubica
AU  - Pavlović, Ivan
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2019
UR  - https://www.mdpi.com/2076-3921/8/4/97
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8403
AB  - We previously found that compared to patients with benign uterine diseases (polyps, myomas), patients with premalignant (hyperplasia simplex and complex) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme (AOE) activities. To further elucidate the mechanism of the observed changes, we examined protein and mRNA levels of copper-zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and transcription factor Nrf2. We also examined correlations of AOE expression with AOE activity, lipid hydroperoxides (LOOH) level, and level of Nrf2. Our results showed decreased CuZnSOD, CAT, and Nrf2 levels, and increased GPx and GR levels in hyperplasias, while in patients with adenocarcinoma, the level of CAT was decreased and GR was increased, compared to benign groups. Similar changes in mRNA levels were also detected, indicating predominantly translational control of the AOE expression. The positive correlation of enzyme expression/activity was recorded for CuZnSOD, GPx, and GR, but only among groups with benign diseases. Only GR and GPx expressions were positively correlated with LOOH. Nrf2 protein was positively correlated with mRNA levels of CuZnSOD and GR. Observed results indicate involvement of diverse redox mechanisms in etiopathogenesis of different gynecological diseases, and may improve redox-based approaches in current clinical practice.
T2  - Antioxidants
T1  - Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma
VL  - 8
IS  - 4
SP  - 97
DO  - 10.3390/antiox8040097
ER  - 
@article{
author = "Todorović, Ana and Pejić, Snežana and Gavrilović, Ljubica and Pavlović, Ivan and Stojiljković, Vesna and Popović, Nataša M. and Pajović, Snežana B.",
year = "2019",
url = "https://www.mdpi.com/2076-3921/8/4/97, http://vinar.vin.bg.ac.rs/handle/123456789/8403",
abstract = "We previously found that compared to patients with benign uterine diseases (polyps, myomas), patients with premalignant (hyperplasia simplex and complex) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme (AOE) activities. To further elucidate the mechanism of the observed changes, we examined protein and mRNA levels of copper-zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and transcription factor Nrf2. We also examined correlations of AOE expression with AOE activity, lipid hydroperoxides (LOOH) level, and level of Nrf2. Our results showed decreased CuZnSOD, CAT, and Nrf2 levels, and increased GPx and GR levels in hyperplasias, while in patients with adenocarcinoma, the level of CAT was decreased and GR was increased, compared to benign groups. Similar changes in mRNA levels were also detected, indicating predominantly translational control of the AOE expression. The positive correlation of enzyme expression/activity was recorded for CuZnSOD, GPx, and GR, but only among groups with benign diseases. Only GR and GPx expressions were positively correlated with LOOH. Nrf2 protein was positively correlated with mRNA levels of CuZnSOD and GR. Observed results indicate involvement of diverse redox mechanisms in etiopathogenesis of different gynecological diseases, and may improve redox-based approaches in current clinical practice.",
journal = "Antioxidants",
title = "Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma",
volume = "8",
number = "4",
pages = "97",
doi = "10.3390/antiox8040097"
}
Todorović, A., Pejić, S., Gavrilović, L., Pavlović, I., Stojiljković, V., Popović, N. M.,& Pajović, S. B. (2019). Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma.
Antioxidants, 8(4), 97.
https://doi.org/10.3390/antiox8040097
Todorović A, Pejić S, Gavrilović L, Pavlović I, Stojiljković V, Popović NM, Pajović SB. Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma. Antioxidants. 2019;8(4):97
Todorović Ana, Pejić Snežana, Gavrilović Ljubica, Pavlović Ivan, Stojiljković Vesna, Popović Nataša M., Pajović Snežana B., "Expression of Antioxidant Enzymes in Patients with Uterine Polyp, Myoma, Hyperplasia, and Adenocarcinoma" Antioxidants, 8, no. 4 (2019):97,
https://doi.org/10.3390/antiox8040097 .
4
4
4

Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats

Popović, Nataša M.; Pajović, Snežana B.; Stojiljković, Vesna; Todorović, Ana; Pejić, Snežana; Pavlović, Ivan; Gavrilović, Ljubica

(2018)

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8404
AB  - The effects of chronic restraint stress (CRS, 2 h during 14 days) on gene expression of tyrosine hydroxylase (TH), catechol-O-methyltransferase (COMT), and glutathione peroxidase (GPx) were studied in the rat hippocampus. Changes in the dopamine (DA) concentration and activities of monoamine oxidases (MAO A and MAO B) and GPx in this cerebral structure of chronically stressed rats were also examined. The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assay of enzymatic activity. We found that CRS decreased the TH protein level and DA concentration, which probably confirms the statement that de novo synthesis of DA is suppressed under stress conditions. The increased activities of MAO B, as well as the increased level of COMT protein, are believed to be related to intensified DA catabolism conditions. Also, a decreased activity of GPx in the hippocampus of chronically stressed animals was found. The increased enzymatic activity of MAO B negatively correlated with the reduced activity of GPx under the above-mentioned stress conditions. These events in the hippocampus of chronically stressed animals could synergistically cause oxidative damage to the mitochondria. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Neurophysiology
T1  - Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats
VL  - 50
IS  - 5
SP  - 332
EP  - 338
DO  - 10.1007/s11062-019-09758-z
ER  - 
@article{
author = "Popović, Nataša M. and Pajović, Snežana B. and Stojiljković, Vesna and Todorović, Ana and Pejić, Snežana and Pavlović, Ivan and Gavrilović, Ljubica",
year = "2018",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8404",
abstract = "The effects of chronic restraint stress (CRS, 2 h during 14 days) on gene expression of tyrosine hydroxylase (TH), catechol-O-methyltransferase (COMT), and glutathione peroxidase (GPx) were studied in the rat hippocampus. Changes in the dopamine (DA) concentration and activities of monoamine oxidases (MAO A and MAO B) and GPx in this cerebral structure of chronically stressed rats were also examined. The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assay of enzymatic activity. We found that CRS decreased the TH protein level and DA concentration, which probably confirms the statement that de novo synthesis of DA is suppressed under stress conditions. The increased activities of MAO B, as well as the increased level of COMT protein, are believed to be related to intensified DA catabolism conditions. Also, a decreased activity of GPx in the hippocampus of chronically stressed animals was found. The increased enzymatic activity of MAO B negatively correlated with the reduced activity of GPx under the above-mentioned stress conditions. These events in the hippocampus of chronically stressed animals could synergistically cause oxidative damage to the mitochondria. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Neurophysiology",
title = "Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats",
volume = "50",
number = "5",
pages = "332-338",
doi = "10.1007/s11062-019-09758-z"
}
Popović, N. M., Pajović, S. B., Stojiljković, V., Todorović, A., Pejić, S., Pavlović, I.,& Gavrilović, L. (2018). Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats.
Neurophysiology, 50(5), 332-338.
https://doi.org/10.1007/s11062-019-09758-z
Popović NM, Pajović SB, Stojiljković V, Todorović A, Pejić S, Pavlović I, Gavrilović L. Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats. Neurophysiology. 2018;50(5):332-338
Popović Nataša M., Pajović Snežana B., Stojiljković Vesna, Todorović Ana, Pejić Snežana, Pavlović Ivan, Gavrilović Ljubica, "Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats" Neurophysiology, 50, no. 5 (2018):332-338,
https://doi.org/10.1007/s11062-019-09758-z .

Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System

Bartholomew, Ibeh; Gavrilović, Ljubica; Stojiljković, Vesna; Popović, Nataša M.; Pejić, Snežana; Todorović, Ana; Pavlović, Ivan; Pajović, Snežana B.

(IntechOpen, 2018)

TY  - CHAP
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8406
AB  - We examined the effects of daily exercise on the gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), and phenyl
ethanolamine N-methyltransferase (PNMT)), vesicular monoamine transporter 2 (VMAT 2),
antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), concentrations of catecholamines (noradrenaline (NA) and adrenaline (A))
and malondialdehyde (MDA), activities of monoamine oxidase (MAO), and antioxidant
enzymes in the spleen of chronically psychosocially stressed rats. Exposure of chronically
stressed rats to exercise increased the levels of PNMT protein by 19%, VMAT 2 mRNA by
100%, NA by 160%, and A by 140%; decreased/unchanged MAO enzyme activity; returned
concentrations of MDA to control level; and increased CAT and GPx mRNA levels (50%
and 150%, respectively). Exercise induced the accumulation of the catecholamines and a
decrease of stress-induced oxidative stress in the spleen, which may significantly affect the
immune-neuroendocrine interactions in stress conditions. Also, exercise induced the catecholaminergic system and antioxidant defense to become more ready to a novel stressor,
which indicates that exercise may induce potentially positive physiological adaptations.
Our combined model of chronic social isolation and long-term daily treadmill running in
rats may be a good animal model in the research of therapeutic role of exercise in human
disease caused by chronic stress.
PB  - IntechOpen
PB  - InTech
T2  - Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh
T1  - Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System
IS  - Ch. 14
SP  - 283
EP  - 310
DO  - 10.5772/intechopen.70008
ER  - 
@article{
editor = "Bartholomew, Ibeh",
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Popović, Nataša M. and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pajović, Snežana B.",
year = "2018",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8406",
abstract = "We examined the effects of daily exercise on the gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), and phenyl
ethanolamine N-methyltransferase (PNMT)), vesicular monoamine transporter 2 (VMAT 2),
antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), concentrations of catecholamines (noradrenaline (NA) and adrenaline (A))
and malondialdehyde (MDA), activities of monoamine oxidase (MAO), and antioxidant
enzymes in the spleen of chronically psychosocially stressed rats. Exposure of chronically
stressed rats to exercise increased the levels of PNMT protein by 19%, VMAT 2 mRNA by
100%, NA by 160%, and A by 140%; decreased/unchanged MAO enzyme activity; returned
concentrations of MDA to control level; and increased CAT and GPx mRNA levels (50%
and 150%, respectively). Exercise induced the accumulation of the catecholamines and a
decrease of stress-induced oxidative stress in the spleen, which may significantly affect the
immune-neuroendocrine interactions in stress conditions. Also, exercise induced the catecholaminergic system and antioxidant defense to become more ready to a novel stressor,
which indicates that exercise may induce potentially positive physiological adaptations.
Our combined model of chronic social isolation and long-term daily treadmill running in
rats may be a good animal model in the research of therapeutic role of exercise in human
disease caused by chronic stress.",
publisher = "IntechOpen, InTech",
journal = "Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh",
title = "Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System",
number = "Ch. 14",
pages = "283-310",
doi = "10.5772/intechopen.70008"
}
Bartholomew, I., Gavrilović, L., Stojiljković, V., Popović, N. M., Pejić, S., Todorović, A., Pavlović, I.,& Pajović, S. B. (2018). Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System.
Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh
InTech.(Ch. 14), 283-310.
https://doi.org/10.5772/intechopen.70008
Bartholomew I, Gavrilović L, Stojiljković V, Popović NM, Pejić S, Todorović A, Pavlović I, Pajović SB. Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System. Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh. 2018;(Ch. 14):283-310
Bartholomew Ibeh, Gavrilović Ljubica, Stojiljković Vesna, Popović Nataša M., Pejić Snežana, Todorović Ana, Pavlović Ivan, Pajović Snežana B., "Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System" Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh, no. Ch. 14 (2018):283-310,
https://doi.org/10.5772/intechopen.70008 .
2

Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats

Popović, Nataša M.; Pajović, Snežana B.; Stojiljković, Vesna; Pejić, Snežana; Todorović, Ana; Pavlović, Ivan; Gavrilović, Ljubica

(2017)

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1588
AB  - This study examined the effects of chronic restraint stress (CRS) on gene expression of tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH), dopamine transporter (DAT), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2), catechol-O-methyltransferase (COMT) and antioxidant enzymes such as superoxide dismutase (SOD1 and SOD2), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), as well as concentrations of dopamine (DA) and noradrenaline (NA), activities of monoamine oxidase (MAO A and MAOB) and activities of antioxidant enzymes (SOD1, SOD2, CAT, GPx and GR) in the rat prefrontal cortex (PFC). We found that CRS decreases gene expression of TH and DBH and concentrations of DA, which probably confirms the decrease of de novo synthesis of catecholamine. CRS increased protein levels of NET and VMAT2, which was followed by increased NA concentration. The increased activity of MAO A and MAO B, as well as increased protein levels of COMT probably indicate increased catecholamine degradation, which was followed by increased activity of SOD1, SOD2 and CAT, as well as decreased activity of GPx under stress conditions. Our findings confirm the increased prefrontal noradrenergic turnover in animals exposed to CRS. The molecular mechanisms by which CRS changes catecholaminergic turnover and the antioxidant defense system in the PFC may be very important for research on numerous psychiatric diseases caused by chronic stress.
T2  - Folia Biologica, Krakow
T1  - Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats
VL  - 65
IS  - 1
SP  - 43
EP  - 54
DO  - 10.3409/fb65_1.43
ER  - 
@article{
author = "Popović, Nataša M. and Pajović, Snežana B. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Gavrilović, Ljubica",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1588",
abstract = "This study examined the effects of chronic restraint stress (CRS) on gene expression of tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH), dopamine transporter (DAT), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2), catechol-O-methyltransferase (COMT) and antioxidant enzymes such as superoxide dismutase (SOD1 and SOD2), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), as well as concentrations of dopamine (DA) and noradrenaline (NA), activities of monoamine oxidase (MAO A and MAOB) and activities of antioxidant enzymes (SOD1, SOD2, CAT, GPx and GR) in the rat prefrontal cortex (PFC). We found that CRS decreases gene expression of TH and DBH and concentrations of DA, which probably confirms the decrease of de novo synthesis of catecholamine. CRS increased protein levels of NET and VMAT2, which was followed by increased NA concentration. The increased activity of MAO A and MAO B, as well as increased protein levels of COMT probably indicate increased catecholamine degradation, which was followed by increased activity of SOD1, SOD2 and CAT, as well as decreased activity of GPx under stress conditions. Our findings confirm the increased prefrontal noradrenergic turnover in animals exposed to CRS. The molecular mechanisms by which CRS changes catecholaminergic turnover and the antioxidant defense system in the PFC may be very important for research on numerous psychiatric diseases caused by chronic stress.",
journal = "Folia Biologica, Krakow",
title = "Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats",
volume = "65",
number = "1",
pages = "43-54",
doi = "10.3409/fb65_1.43"
}
Popović, N. M., Pajović, S. B., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I.,& Gavrilović, L. (2017). Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats.
Folia Biologica, Krakow, 65(1), 43-54.
https://doi.org/10.3409/fb65_1.43
Popović NM, Pajović SB, Stojiljković V, Pejić S, Todorović A, Pavlović I, Gavrilović L. Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats. Folia Biologica, Krakow. 2017;65(1):43-54
Popović Nataša M., Pajović Snežana B., Stojiljković Vesna, Pejić Snežana, Todorović Ana, Pavlović Ivan, Gavrilović Ljubica, "Prefrontal Catecholaminergic Turnover and Antioxidant Defense System of Chronically Stressed Rats" Folia Biologica, Krakow, 65, no. 1 (2017):43-54,
https://doi.org/10.3409/fb65_1.43 .
4
5
5

Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis

Pavlović, Ivan; Pejić, Snežana; Glumac, Sofija; Todorović, Ana; Stojiljković, Vesna; Popović, Nataša M.; Gavrilović, Ljubica; Pajović, Snežana B.; Radojevic-Skodric, Sanja; Dzamic, Zoran; Basta-Jovanović, Gordana

(2017)

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Pejić, Snežana
AU  - Glumac, Sofija
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Gavrilović, Ljubica
AU  - Pajović, Snežana B.
AU  - Radojevic-Skodric, Sanja
AU  - Dzamic, Zoran
AU  - Basta-Jovanović, Gordana
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1857
AB  - Purpose: Indications of kidney cancer outcome in lower-income countries are based on an incidence/mortality ratio due to lack of survival information. This study was conducted to provide outcome data in Serbian patients with renal cell carcinoma (RCC) and to identify prognostic factors that could affect their overall survival (OS). Methods: This retrospective study included 185 patients who underwent nephrectomy. We assessed certain clinicopathological data including age, gender, tumor size, grade, stage and histological subtypes for their possible impact on OS. Results: The 5-year OS was 63.2%. Significant association was found between OS and age (log-rank 12.455, p=0.006), tumor size (log-rank 26.425, p=0.000), grade (log-rank 13.249, p=0.000) and stage (log-rank 43.235, p=0.000). Univariate analysis indicated size (p=0.000), grade (p=0.001) and stage (p=0.000) as prognostic factors for OS. In multivariate analysis, grade (p=0.014) and stage (p=0.000) remained significant predictors of OS. Conclusion: Tumor grade and stage were identified as independent prognostic factors of OS survival in Serbian patients with RCC.
T2  - Journal of BUON
T1  - Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis
VL  - 22
IS  - 6
SP  - 1434
EP  - 1440
ER  - 
@article{
author = "Pavlović, Ivan and Pejić, Snežana and Glumac, Sofija and Todorović, Ana and Stojiljković, Vesna and Popović, Nataša M. and Gavrilović, Ljubica and Pajović, Snežana B. and Radojevic-Skodric, Sanja and Dzamic, Zoran and Basta-Jovanović, Gordana",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1857",
abstract = "Purpose: Indications of kidney cancer outcome in lower-income countries are based on an incidence/mortality ratio due to lack of survival information. This study was conducted to provide outcome data in Serbian patients with renal cell carcinoma (RCC) and to identify prognostic factors that could affect their overall survival (OS). Methods: This retrospective study included 185 patients who underwent nephrectomy. We assessed certain clinicopathological data including age, gender, tumor size, grade, stage and histological subtypes for their possible impact on OS. Results: The 5-year OS was 63.2%. Significant association was found between OS and age (log-rank 12.455, p=0.006), tumor size (log-rank 26.425, p=0.000), grade (log-rank 13.249, p=0.000) and stage (log-rank 43.235, p=0.000). Univariate analysis indicated size (p=0.000), grade (p=0.001) and stage (p=0.000) as prognostic factors for OS. In multivariate analysis, grade (p=0.014) and stage (p=0.000) remained significant predictors of OS. Conclusion: Tumor grade and stage were identified as independent prognostic factors of OS survival in Serbian patients with RCC.",
journal = "Journal of BUON",
title = "Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis",
volume = "22",
number = "6",
pages = "1434-1440"
}
Pavlović, I., Pejić, S., Glumac, S., Todorović, A., Stojiljković, V., Popović, N. M., Gavrilović, L., Pajović, S. B., Radojevic-Skodric, S., Dzamic, Z.,& Basta-Jovanović, G. (2017). Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis.
Journal of BUON, 22(6), 1434-1440.
Pavlović I, Pejić S, Glumac S, Todorović A, Stojiljković V, Popović NM, Gavrilović L, Pajović SB, Radojevic-Skodric S, Dzamic Z, Basta-Jovanović G. Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis. Journal of BUON. 2017;22(6):1434-1440
Pavlović Ivan, Pejić Snežana, Glumac Sofija, Todorović Ana, Stojiljković Vesna, Popović Nataša M., Gavrilović Ljubica, Pajović Snežana B., Radojevic-Skodric Sanja, Dzamic Zoran, Basta-Jovanović Gordana, "Clinicopathological characteristics and survival in Serbian patients with renal cell carcinoma: a retrospective analysis" Journal of BUON, 22, no. 6 (2017):1434-1440
2
2

Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats

Popović, Nataša M.; Pajović, Snežana B.; Stojiljković, Vesna; Todorović, Ana; Pejić, Snežana; Pavlović, Ivan; Gavrilović, Ljubica

(2017)

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1908
AB  - This study examined the effects of chronic restraint stress (CRS: 2 hours x 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantified by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our findings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.
T2  - Acta Veterinaria, Beograd
T1  - Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats
VL  - 67
IS  - 4
SP  - 540
EP  - 550
DO  - 10.1515/acve-2017-0043
ER  - 
@article{
author = "Popović, Nataša M. and Pajović, Snežana B. and Stojiljković, Vesna and Todorović, Ana and Pejić, Snežana and Pavlović, Ivan and Gavrilović, Ljubica",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1908",
abstract = "This study examined the effects of chronic restraint stress (CRS: 2 hours x 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantified by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our findings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.",
journal = "Acta Veterinaria, Beograd",
title = "Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats",
volume = "67",
number = "4",
pages = "540-550",
doi = "10.1515/acve-2017-0043"
}
Popović, N. M., Pajović, S. B., Stojiljković, V., Todorović, A., Pejić, S., Pavlović, I.,& Gavrilović, L. (2017). Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats.
Acta Veterinaria, Beograd, 67(4), 540-550.
https://doi.org/10.1515/acve-2017-0043
Popović NM, Pajović SB, Stojiljković V, Todorović A, Pejić S, Pavlović I, Gavrilović L. Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats. Acta Veterinaria, Beograd. 2017;67(4):540-550
Popović Nataša M., Pajović Snežana B., Stojiljković Vesna, Todorović Ana, Pejić Snežana, Pavlović Ivan, Gavrilović Ljubica, "Increased Activity of Hippocampal Antioxidant Enzymes as An Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats" Acta Veterinaria, Beograd, 67, no. 4 (2017):540-550,
https://doi.org/10.1515/acve-2017-0043 .
3
3
4

Superoxide dismutase and lipid peroxidation in children affected by celiac disease

Stojiljković, Vesna; Gavrilović, Ljubica; Pejić, Snežana; Todorović, Ana; Popović, Nataša M.; Pavlović, Ivan; Pajović, Snežana B.

(RAD Association, 2017)

TY  - CONF
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2017
UR  - http://www.rad-proceedings.org/paper.php?id=152
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7995
AB  - Celiac disease (CD) is an autoimmune disorder provoked by the wheat gluten and related proteins from other grains. The only treatment for the patients is a lifelong gluten free diet (GFD). Oxidative stress has been implicated in the pathogenesis of CD. The aim of this study was to examine the modulation of the biochemical response to oxidative stress in children affected by CD. The study involved peripheral blood samples and small intestinal biopsies from 69 children diagnosed with CD. According to the histological findings, patients were divided into following groups: Marsh 0: normal mucosa with no signs of inflammation (n=31); Marsh 1: mucosa was characterized by intraepithelial lymphocytosis (n=5); Marsh 2: intraepithelial lymphocytosis was accompanied by crypt hyperplasia (n=4); Marsh 3a: mucosa showed partial villous atrophy (n=20); Marsh 3b: subtotal villous atrophy was present (n=9). For the statistical purposes groups Marsh 1 and Marsh 2 were treated as one (Marsh 1+2, n=9). The activities and protein levels of copper, zinc superoxide dismutase (CuZnSOD) and manganese SOD (MnSOD), as well as the concentrations of lipid hydroperoxides (LOOH) were determined in intestinal biopsies, while in the peripheral blood, MnSOD activity was not measured, due to the methodological obstacles. CuZnSOD activity in the blood varied significantly between the analyzed groups. Marsh 3a and Marsh 3b had increased CuZnSOD activity comparing to the Marsh 0 (P < 0.05). LOOH concentration also varied significantly. LOOH level was higher in the blood of Marsh 3a (P < 0.001) and Marsh 3b (P < 0.05), than in Marsh 0. In the biopsy samples, MnSOD activity and LOOH concentration showed significant differences between the groups, while no significant difference was found for CuZnSOD activity. In comparison to Marsh 0, MnSOD activity was significantly elevated in Marsh 3a (P < 0.01). Significant increase in LOOH concentration was found in Marsh 3a (P < 0.001) and Marsh 3b (P < 0.01), comparing to Marsh 0. In addition, Marsh 3a group had higher LOOH concentration than Marsh 1+2. Relative MnSOD and CuZnSOD protein level in the peripheral blood and intestinal mucosa did not vary significantly between the analyzed groups. Positive correlations were found between the severity of mucosal lesion and CuZnSOD activity (P < 0.001), as well as LOOH concentration (P < 0.001) in the peripheral blood. Similar correlations were found also in intestinal mucosa: MnSOD: P < 0.05; CuZnSOD: P < 0.05; LOOH: P < 0.001. Our results show that oxidant/antioxidant balance is disturbed in CD patients with mucosal lesions. An increase in SOD activity as a consequence of oxidant pressure is not enough to maintain the normal level of free radicals, which leads to enhanced lipid peroxidation. These processes persist even in some patients on a long-term GFD.
PB  - RAD Association
C3  - RAD Conference Proceedings
T1  - Superoxide dismutase and lipid peroxidation in children affected by celiac disease
VL  - 2
SP  - 237
EP  - 242
DO  - 10.21175/RadProc.2017.49
ER  - 
@conference{
author = "Stojiljković, Vesna and Gavrilović, Ljubica and Pejić, Snežana and Todorović, Ana and Popović, Nataša M. and Pavlović, Ivan and Pajović, Snežana B.",
year = "2017",
url = "http://www.rad-proceedings.org/paper.php?id=152, http://vinar.vin.bg.ac.rs/handle/123456789/7995",
abstract = "Celiac disease (CD) is an autoimmune disorder provoked by the wheat gluten and related proteins from other grains. The only treatment for the patients is a lifelong gluten free diet (GFD). Oxidative stress has been implicated in the pathogenesis of CD. The aim of this study was to examine the modulation of the biochemical response to oxidative stress in children affected by CD. The study involved peripheral blood samples and small intestinal biopsies from 69 children diagnosed with CD. According to the histological findings, patients were divided into following groups: Marsh 0: normal mucosa with no signs of inflammation (n=31); Marsh 1: mucosa was characterized by intraepithelial lymphocytosis (n=5); Marsh 2: intraepithelial lymphocytosis was accompanied by crypt hyperplasia (n=4); Marsh 3a: mucosa showed partial villous atrophy (n=20); Marsh 3b: subtotal villous atrophy was present (n=9). For the statistical purposes groups Marsh 1 and Marsh 2 were treated as one (Marsh 1+2, n=9). The activities and protein levels of copper, zinc superoxide dismutase (CuZnSOD) and manganese SOD (MnSOD), as well as the concentrations of lipid hydroperoxides (LOOH) were determined in intestinal biopsies, while in the peripheral blood, MnSOD activity was not measured, due to the methodological obstacles. CuZnSOD activity in the blood varied significantly between the analyzed groups. Marsh 3a and Marsh 3b had increased CuZnSOD activity comparing to the Marsh 0 (P < 0.05). LOOH concentration also varied significantly. LOOH level was higher in the blood of Marsh 3a (P < 0.001) and Marsh 3b (P < 0.05), than in Marsh 0. In the biopsy samples, MnSOD activity and LOOH concentration showed significant differences between the groups, while no significant difference was found for CuZnSOD activity. In comparison to Marsh 0, MnSOD activity was significantly elevated in Marsh 3a (P < 0.01). Significant increase in LOOH concentration was found in Marsh 3a (P < 0.001) and Marsh 3b (P < 0.01), comparing to Marsh 0. In addition, Marsh 3a group had higher LOOH concentration than Marsh 1+2. Relative MnSOD and CuZnSOD protein level in the peripheral blood and intestinal mucosa did not vary significantly between the analyzed groups. Positive correlations were found between the severity of mucosal lesion and CuZnSOD activity (P < 0.001), as well as LOOH concentration (P < 0.001) in the peripheral blood. Similar correlations were found also in intestinal mucosa: MnSOD: P < 0.05; CuZnSOD: P < 0.05; LOOH: P < 0.001. Our results show that oxidant/antioxidant balance is disturbed in CD patients with mucosal lesions. An increase in SOD activity as a consequence of oxidant pressure is not enough to maintain the normal level of free radicals, which leads to enhanced lipid peroxidation. These processes persist even in some patients on a long-term GFD.",
publisher = "RAD Association",
journal = "RAD Conference Proceedings",
title = "Superoxide dismutase and lipid peroxidation in children affected by celiac disease",
volume = "2",
pages = "237-242",
doi = "10.21175/RadProc.2017.49"
}
Stojiljković, V., Gavrilović, L., Pejić, S., Todorović, A., Popović, N. M., Pavlović, I.,& Pajović, S. B. (2017). Superoxide dismutase and lipid peroxidation in children affected by celiac disease.
RAD Conference Proceedings
RAD Association., 2, 237-242.
https://doi.org/10.21175/RadProc.2017.49
Stojiljković V, Gavrilović L, Pejić S, Todorović A, Popović NM, Pavlović I, Pajović SB. Superoxide dismutase and lipid peroxidation in children affected by celiac disease. RAD Conference Proceedings. 2017;2:237-242
Stojiljković Vesna, Gavrilović Ljubica, Pejić Snežana, Todorović Ana, Popović Nataša M., Pavlović Ivan, Pajović Snežana B., "Superoxide dismutase and lipid peroxidation in children affected by celiac disease" RAD Conference Proceedings, 2 (2017):237-242,
https://doi.org/10.21175/RadProc.2017.49 .

Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats

Popović, Nataša M.; Pajović, Snežana B.; Stojiljković, Vesna; Todorović, Ana; Pejić, Snežana; Pavlović, Ivan; Gavrilović, Ljubica

(RAD Association, 2017)

TY  - CONF
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
PY  - 2017
UR  - http://www.rad-proceedings.org/paper.php?id=155
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7996
AB  - Chronic stress induces over-activation and dysfunction of stress-activated systems, resulting in further brain damage and depressive-like behavior. Depression is a potentially life-threatening disorder that affects people and, therefore, it is one of the most important public health problems. This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on the anxiety-like and depression-like behaviors in rats, as well as on the possible changes in the concentrations of dopamine (DA) and noradrenaline (NA) in the prefrontal cortex and hippocampus. We observed a decrease in the number of entries into open arms and time spent in open arms during the elevated plus-maze test (anxiety-like behavior), as well as the increased immobility during the forced swimming test (depression-like behavior). In addition, we found that CRS increases concentration of NA and decreases concentration of DA in the prefrontal cortex and hippocampus. Also, we recorded a significant correlation between the animal behavior and levels of neurotransmitters in the prefrontal cortex and hippocampus in stress conditions provoked by CRS. The results presented here suggest that there is a relationship between the animal behavior and levels of neurotransmitters in the prefrontal cortex and hippocampus in stress conditions provoked by CRS, which may be important in the research of numerous psychiatric diseases caused by chronic stress. © RAD Conference Proceedings. All rights reserved.
PB  - RAD Association
C3  - RAD Conference Proceedings
T1  - Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats
VL  - 2
SP  - 255
EP  - 259
DO  - 10.21175/RadProc.2017.52
ER  - 
@conference{
author = "Popović, Nataša M. and Pajović, Snežana B. and Stojiljković, Vesna and Todorović, Ana and Pejić, Snežana and Pavlović, Ivan and Gavrilović, Ljubica",
year = "2017",
url = "http://www.rad-proceedings.org/paper.php?id=155, http://vinar.vin.bg.ac.rs/handle/123456789/7996",
abstract = "Chronic stress induces over-activation and dysfunction of stress-activated systems, resulting in further brain damage and depressive-like behavior. Depression is a potentially life-threatening disorder that affects people and, therefore, it is one of the most important public health problems. This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on the anxiety-like and depression-like behaviors in rats, as well as on the possible changes in the concentrations of dopamine (DA) and noradrenaline (NA) in the prefrontal cortex and hippocampus. We observed a decrease in the number of entries into open arms and time spent in open arms during the elevated plus-maze test (anxiety-like behavior), as well as the increased immobility during the forced swimming test (depression-like behavior). In addition, we found that CRS increases concentration of NA and decreases concentration of DA in the prefrontal cortex and hippocampus. Also, we recorded a significant correlation between the animal behavior and levels of neurotransmitters in the prefrontal cortex and hippocampus in stress conditions provoked by CRS. The results presented here suggest that there is a relationship between the animal behavior and levels of neurotransmitters in the prefrontal cortex and hippocampus in stress conditions provoked by CRS, which may be important in the research of numerous psychiatric diseases caused by chronic stress. © RAD Conference Proceedings. All rights reserved.",
publisher = "RAD Association",
journal = "RAD Conference Proceedings",
title = "Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats",
volume = "2",
pages = "255-259",
doi = "10.21175/RadProc.2017.52"
}
Popović, N. M., Pajović, S. B., Stojiljković, V., Todorović, A., Pejić, S., Pavlović, I.,& Gavrilović, L. (2017). Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats.
RAD Conference Proceedings
RAD Association., 2, 255-259.
https://doi.org/10.21175/RadProc.2017.52
Popović NM, Pajović SB, Stojiljković V, Todorović A, Pejić S, Pavlović I, Gavrilović L. Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats. RAD Conference Proceedings. 2017;2:255-259
Popović Nataša M., Pajović Snežana B., Stojiljković Vesna, Todorović Ana, Pejić Snežana, Pavlović Ivan, Gavrilović Ljubica, "Relationship between behaviors and catecholamine content in prefrontal cortex and hippocampus of chronically stressed rats" RAD Conference Proceedings, 2 (2017):255-259,
https://doi.org/10.21175/RadProc.2017.52 .
3
3

Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma

Pavlović, Ivan; Todorović, Ana; Stojiljković, Vesna; Gavrilović, Ljubica; Popović, Nataša M.; Pajović, Snežana B.; Pejić, Snežana

(2016)

TY  - CONF
AU  - Pavlović, Ivan
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Pejić, Snežana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7171
AB  - Renal cell carcinoma is the most common and the most lethal type of kidney cancer with the highest incidence in developed countries. At the moment of diagnosis, many patients already have developed metastases. Tobacco smoking, obesity, hypertension and occupational exposure are established risk factors. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) are antioxidant enzymes (AOE) that have a key role in protecting from reactive oxygen species (ROS) produced in pathological processes. We examined the antioxidant capacity of tumour tissue in patients with clear cell type of renal cell carcinoma. The results showed a significant decrease of AOE activities in the tumour tissue compared to the normal kidney tissue, indicating that the tumour tissue is under permanent oxidative stress. Renal cell carcinoma is highly resistant to radiation therapy, which is also associated with free radical production. Since the tumour tissue has impaired AO capacity, the findings of this study may contribute to the improvement of the potential therapeutic treatments.
C3  - RAD Conference Proceedings
T1  - Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma
SP  - 153
EP  - 155
DO  - 10.21175/RadProc.2016.36
ER  - 
@conference{
author = "Pavlović, Ivan and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B. and Pejić, Snežana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7171",
abstract = "Renal cell carcinoma is the most common and the most lethal type of kidney cancer with the highest incidence in developed countries. At the moment of diagnosis, many patients already have developed metastases. Tobacco smoking, obesity, hypertension and occupational exposure are established risk factors. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) are antioxidant enzymes (AOE) that have a key role in protecting from reactive oxygen species (ROS) produced in pathological processes. We examined the antioxidant capacity of tumour tissue in patients with clear cell type of renal cell carcinoma. The results showed a significant decrease of AOE activities in the tumour tissue compared to the normal kidney tissue, indicating that the tumour tissue is under permanent oxidative stress. Renal cell carcinoma is highly resistant to radiation therapy, which is also associated with free radical production. Since the tumour tissue has impaired AO capacity, the findings of this study may contribute to the improvement of the potential therapeutic treatments.",
journal = "RAD Conference Proceedings",
title = "Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma",
pages = "153-155",
doi = "10.21175/RadProc.2016.36"
}
Pavlović, I., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M., Pajović, S. B.,& Pejić, S. (2016). Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma.
RAD Conference Proceedings, 153-155.
https://doi.org/10.21175/RadProc.2016.36
Pavlović I, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Pajović SB, Pejić S. Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma. RAD Conference Proceedings. 2016;:153-155
Pavlović Ivan, Todorović Ana, Stojiljković Vesna, Gavrilović Ljubica, Popović Nataša M., Pajović Snežana B., Pejić Snežana, "Antioxidant Capacity of the Kidney Tissue in Patients with Renal Cell Carcinoma" RAD Conference Proceedings (2016):153-155,
https://doi.org/10.21175/RadProc.2016.36 .

Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress

Pejić, Snežana; Stojiljković, Vesna; Todorović, Ana; Gavrilović, Ljubica; Pavlović, Ivan; Popović, Nataša M.; Pajović, Snežana B.

(2016)

TY  - JOUR
AU  - Pejić, Snežana
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Gavrilović, Ljubica
AU  - Pavlović, Ivan
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1301
AB  - The study deals with manganese superoxide dismutase, copper, zinc superoxide dismutase, and catalase activities in brain cortex of Wistar rats exposed to acute stress (immobilization or cold for 2 h), chronic stress (long-term isolation or long-term forced swimming for 21 days), or to combined chronic/acute stress. We observed that i) single episodes of acute stress by immobilization increased activity of both superoxide dismutases; ii) both types of chronic stresses significantly elevated activities of all examined enzymes; iii) chronic social isolation was a much stronger stressor than physical stress by swimming; iv) in animals pre-exposed to chronic isolation, additional stress by immobilization or cold significantly decreased previously elevated activities of all enzymes, while after chronic swimming, acute immobilization lowered only catalase activity. The obtained results indicate that stress conditions most probably altered the cell redox equilibrium, thus influencing the antioxidant response in brain cortex. Further investigation of neuronal prooxidant/antioxidant cellular conditions is needed to improve the prevention and treatment of various stress induced diseases.
T2  - Folia Biologica, Krakow
T1  - Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress
VL  - 64
IS  - 3
SP  - 189
EP  - 195
DO  - 10.3409/fb64_3.189
ER  - 
@article{
author = "Pejić, Snežana and Stojiljković, Vesna and Todorović, Ana and Gavrilović, Ljubica and Pavlović, Ivan and Popović, Nataša M. and Pajović, Snežana B.",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1301",
abstract = "The study deals with manganese superoxide dismutase, copper, zinc superoxide dismutase, and catalase activities in brain cortex of Wistar rats exposed to acute stress (immobilization or cold for 2 h), chronic stress (long-term isolation or long-term forced swimming for 21 days), or to combined chronic/acute stress. We observed that i) single episodes of acute stress by immobilization increased activity of both superoxide dismutases; ii) both types of chronic stresses significantly elevated activities of all examined enzymes; iii) chronic social isolation was a much stronger stressor than physical stress by swimming; iv) in animals pre-exposed to chronic isolation, additional stress by immobilization or cold significantly decreased previously elevated activities of all enzymes, while after chronic swimming, acute immobilization lowered only catalase activity. The obtained results indicate that stress conditions most probably altered the cell redox equilibrium, thus influencing the antioxidant response in brain cortex. Further investigation of neuronal prooxidant/antioxidant cellular conditions is needed to improve the prevention and treatment of various stress induced diseases.",
journal = "Folia Biologica, Krakow",
title = "Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress",
volume = "64",
number = "3",
pages = "189-195",
doi = "10.3409/fb64_3.189"
}
Pejić, S., Stojiljković, V., Todorović, A., Gavrilović, L., Pavlović, I., Popović, N. M.,& Pajović, S. B. (2016). Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress.
Folia Biologica, Krakow, 64(3), 189-195.
https://doi.org/10.3409/fb64_3.189
Pejić S, Stojiljković V, Todorović A, Gavrilović L, Pavlović I, Popović NM, Pajović SB. Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress. Folia Biologica, Krakow. 2016;64(3):189-195
Pejić Snežana, Stojiljković Vesna, Todorović Ana, Gavrilović Ljubica, Pavlović Ivan, Popović Nataša M., Pajović Snežana B., "Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress" Folia Biologica, Krakow, 64, no. 3 (2016):189-195,
https://doi.org/10.3409/fb64_3.189 .
5
3
3

Antioxidant status and sex hormones in women with complex endometrial hyperplasia

Pejić, Snežana; Todorović, Ana; Stojiljković, Vesna; Pavlović, Ivan; Gavrilović, Ljubica; Popović, Nataša M.; Pajović, Snežana B.

(2016)

TY  - JOUR
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1455
AB  - Endometrial tissue is under a strong influence of sex hormones. These hormones are considered as developmental factors of endometrial hyperplasia and endometrial cancer. We examined the influence of gonadotropins (follicle-stimulating and luteinizing hormone) and sex hormones (estradiol, progesterone) on oxidant/antioxidant parameters in blood and endometrial tissue of women with complex endometrial hyperplasia. In blood, superoxide dismutase activity was significantly higher in luteal phase and postmenopause compared to the follicular phase. A significant phase-related difference of glutathione peroxidase and glutathione reductase activity was recorded in the endometrium. Both enzymes had lower activity in luteal phase and postmenopause compared to the follicular phase. The linear regression analysis of individual hormonal variables against antioxidant parameters showed negative correlation between glutathione peroxidase activity and gonadotropin concentrations in the endometrium. The regression of hyperplastic to normal endometrium is the purpose of conservative treatment based on administration of progestogens or gonadotropin-releasing hormone analogues. Our findings indicate that gonadotropins influence the antioxidant enzymes activity in women with complex endometrial hyperplasia, which may affect disease development. Further studies are needed to clarify the molecular basis of hormone action on antioxidant system that may potentially initiate a development of treatments based onredox-dependentmechanism.
T2  - Cellular and Molecular Biology
T1  - Antioxidant status and sex hormones in women with complex endometrial hyperplasia
VL  - 62
IS  - 11
SP  - 51
EP  - 56
DO  - 10.14715/cmb/2016.62.11.9
ER  - 
@article{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Pavlović, Ivan and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1455",
abstract = "Endometrial tissue is under a strong influence of sex hormones. These hormones are considered as developmental factors of endometrial hyperplasia and endometrial cancer. We examined the influence of gonadotropins (follicle-stimulating and luteinizing hormone) and sex hormones (estradiol, progesterone) on oxidant/antioxidant parameters in blood and endometrial tissue of women with complex endometrial hyperplasia. In blood, superoxide dismutase activity was significantly higher in luteal phase and postmenopause compared to the follicular phase. A significant phase-related difference of glutathione peroxidase and glutathione reductase activity was recorded in the endometrium. Both enzymes had lower activity in luteal phase and postmenopause compared to the follicular phase. The linear regression analysis of individual hormonal variables against antioxidant parameters showed negative correlation between glutathione peroxidase activity and gonadotropin concentrations in the endometrium. The regression of hyperplastic to normal endometrium is the purpose of conservative treatment based on administration of progestogens or gonadotropin-releasing hormone analogues. Our findings indicate that gonadotropins influence the antioxidant enzymes activity in women with complex endometrial hyperplasia, which may affect disease development. Further studies are needed to clarify the molecular basis of hormone action on antioxidant system that may potentially initiate a development of treatments based onredox-dependentmechanism.",
journal = "Cellular and Molecular Biology",
title = "Antioxidant status and sex hormones in women with complex endometrial hyperplasia",
volume = "62",
number = "11",
pages = "51-56",
doi = "10.14715/cmb/2016.62.11.9"
}
Pejić, S., Todorović, A., Stojiljković, V., Pavlović, I., Gavrilović, L., Popović, N. M.,& Pajović, S. B. (2016). Antioxidant status and sex hormones in women with complex endometrial hyperplasia.
Cellular and Molecular Biology, 62(11), 51-56.
https://doi.org/10.14715/cmb/2016.62.11.9
Pejić S, Todorović A, Stojiljković V, Pavlović I, Gavrilović L, Popović NM, Pajović SB. Antioxidant status and sex hormones in women with complex endometrial hyperplasia. Cellular and Molecular Biology. 2016;62(11):51-56
Pejić Snežana, Todorović Ana, Stojiljković Vesna, Pavlović Ivan, Gavrilović Ljubica, Popović Nataša M., Pajović Snežana B., "Antioxidant status and sex hormones in women with complex endometrial hyperplasia" Cellular and Molecular Biology, 62, no. 11 (2016):51-56,
https://doi.org/10.14715/cmb/2016.62.11.9 .
2
2

Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones

Pejić, Snežana; Todorović, Ana; Stojiljković, Vesna; Gavrilović, Ljubica; Popović, Nataša M.; Pavlović, Ivan; Pajović, Snežana B.

(2015)

TY  - CONF
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7131
AB  - Endometrial hyperplasia complex is gynecological disorder characterized by morphological irregularities of glands shape and size. Antioxidant enzymes (AOE), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), have an essential role in preventing oxidative damage in cell caused by reactive oxygen species (ROS). In this study, we examined the AO status in hyperplastic tissue of patients in menstrual cycle (follicular and luteal phase) and in postmenopause, as well as the relationship between sex hormones and AO parameters. The phase-related activity of GPx and GR in examined patients was significantly different than in healthy women. A significant negative correlation between FSH/LH level and GPx activiy was observed. Endometrial hyperplasias are considered as precancerous lesions and are treated either conservatively or surgicaly, and also by radiation therapy. Since the effects of these therapies are associated with AO and hormonal changes, our results may contribute to the prediction of potential therapeutic efficacy and to selection of the most effective treatment for hyperplasia complex.
C3  - RAD Conference Proceedings
T1  - Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones
SP  - 467
EP  - 470
ER  - 
@conference{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pavlović, Ivan and Pajović, Snežana B.",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7131",
abstract = "Endometrial hyperplasia complex is gynecological disorder characterized by morphological irregularities of glands shape and size. Antioxidant enzymes (AOE), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), have an essential role in preventing oxidative damage in cell caused by reactive oxygen species (ROS). In this study, we examined the AO status in hyperplastic tissue of patients in menstrual cycle (follicular and luteal phase) and in postmenopause, as well as the relationship between sex hormones and AO parameters. The phase-related activity of GPx and GR in examined patients was significantly different than in healthy women. A significant negative correlation between FSH/LH level and GPx activiy was observed. Endometrial hyperplasias are considered as precancerous lesions and are treated either conservatively or surgicaly, and also by radiation therapy. Since the effects of these therapies are associated with AO and hormonal changes, our results may contribute to the prediction of potential therapeutic efficacy and to selection of the most effective treatment for hyperplasia complex.",
journal = "RAD Conference Proceedings",
title = "Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones",
pages = "467-470"
}
Pejić, S., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M., Pavlović, I.,& Pajović, S. B. (2015). Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones.
RAD Conference Proceedings, 467-470.
Pejić S, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Pavlović I, Pajović SB. Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones. RAD Conference Proceedings. 2015;:467-470
Pejić Snežana, Todorović Ana, Stojiljković Vesna, Gavrilović Ljubica, Popović Nataša M., Pavlović Ivan, Pajović Snežana B., "Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones" RAD Conference Proceedings (2015):467-470

Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors

Pejić, Snežana; Stojiljković, Vesna; Todorović, Ana; Gavrilović, Ljubica; Popović, Nataša M.; Pavlović, Ivan; Pajović, Snežana B.

(2015)

TY  - JOUR
AU  - Pejić, Snežana
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/498
AB  - Cancer of the reproductive tract is an important cause of morbidity and mortality among women worldwide. In this study we evaluated the influence of diagnostic categories, age and reproductive factors on antioxidant enzymes and lipid hydroperoxides in the blood of gynaecological patients diagnosed with endometrial polyp, myoma, hyperplasia simplex, hyperplasia complex and endometrial adenocarcinoma. Multivariate regression analysis was used to assess the association of diagnosis, age, parity, abortions and abnormal uterine bleeding with the examined parameters. Diagnosis provided the best predictive model for superoxide dismutase, catalase and glutathione peroxidase activities, and also for the lipid hydroperoxide level. Abortions fitted the best predictive model for superoxide dismutase activity. A significant correlation was also found between the predictor variables themselves. This study showed that reproductive and other factors may be associated, at least partially, with antioxidant capacity and ability to defend against the oxidative damage in gynaecological patients with various diagnoses.
T2  - Folia Biologica
T1  - Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors
VL  - 61
IS  - 1
SP  - 26
EP  - 32
ER  - 
@article{
author = "Pejić, Snežana and Stojiljković, Vesna and Todorović, Ana and Gavrilović, Ljubica and Popović, Nataša M. and Pavlović, Ivan and Pajović, Snežana B.",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/498",
abstract = "Cancer of the reproductive tract is an important cause of morbidity and mortality among women worldwide. In this study we evaluated the influence of diagnostic categories, age and reproductive factors on antioxidant enzymes and lipid hydroperoxides in the blood of gynaecological patients diagnosed with endometrial polyp, myoma, hyperplasia simplex, hyperplasia complex and endometrial adenocarcinoma. Multivariate regression analysis was used to assess the association of diagnosis, age, parity, abortions and abnormal uterine bleeding with the examined parameters. Diagnosis provided the best predictive model for superoxide dismutase, catalase and glutathione peroxidase activities, and also for the lipid hydroperoxide level. Abortions fitted the best predictive model for superoxide dismutase activity. A significant correlation was also found between the predictor variables themselves. This study showed that reproductive and other factors may be associated, at least partially, with antioxidant capacity and ability to defend against the oxidative damage in gynaecological patients with various diagnoses.",
journal = "Folia Biologica",
title = "Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors",
volume = "61",
number = "1",
pages = "26-32"
}
Pejić, S., Stojiljković, V., Todorović, A., Gavrilović, L., Popović, N. M., Pavlović, I.,& Pajović, S. B. (2015). Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors.
Folia Biologica, 61(1), 26-32.
Pejić S, Stojiljković V, Todorović A, Gavrilović L, Popović NM, Pavlović I, Pajović SB. Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors. Folia Biologica. 2015;61(1):26-32
Pejić Snežana, Stojiljković Vesna, Todorović Ana, Gavrilović Ljubica, Popović Nataša M., Pavlović Ivan, Pajović Snežana B., "Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors" Folia Biologica, 61, no. 1 (2015):26-32
4

Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity

Todorović, Ana; Pejić, Snežana; Stojiljković, Vesna; Gavrilović, Ljubica; Popović, Nataša M.; Pavlović, Ivan; Saicic, Zorica S.; Pajović, Snežana B.

(2015)

TY  - JOUR
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Saicic, Zorica S.
AU  - Pajović, Snežana B.
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/815
AB  - Purpose Previously, we examined manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT) activities in rat brain irradiated with 2 or 3 Gy of gamma-rays. The results indicated that lower MnSOD activity and inducibility found in hippocampus might explain higher radiosensitivity of this brain region. Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD, and CAT activities after dose of 5 Gy and to find out if differences in MnSOD activity are caused by changes in its expression. Heads of 4-day-old female rats were irradiated with gamma-rays, using Co-60. Animals were sacrificed 1/24 h after exposure. Hippocampus and cortex tissues were prepared for enzyme activity measurements and Western blot analysis. One hour after exposure, gamma-rays significantly decreased MnSOD activity in both examined brain regions. Twenty-four hours later, MnSOD recovery showed dose and regional dependence. It was weaker at higher doses and in hippocampal region. MnSOD expression changed in the similar manner as MnSOD activity only at lower doses of gamma-rays. In both examined brain regions, gamma radiation significantly decreased CuZnSOD activity and did not change activity of CAT. Our results confirmed that MnSOD plays an important role in different regional radiosensitivity but also showed that depending on dose, radiation affects MnSOD level by utterly different mechanisms. Postradiation changes of CuZnSOD and CAT are not regionally specific and therefore, cannot account for the different radiosensitivity of the hippocampus and cortex.
T2  - Childs Nervous System
T1  - Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity
VL  - 31
IS  - 12
SP  - 2249
EP  - 2256
DO  - 10.1007/s00381-015-2807-2
ER  - 
@article{
author = "Todorović, Ana and Pejić, Snežana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pavlović, Ivan and Saicic, Zorica S. and Pajović, Snežana B.",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/815",
abstract = "Purpose Previously, we examined manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT) activities in rat brain irradiated with 2 or 3 Gy of gamma-rays. The results indicated that lower MnSOD activity and inducibility found in hippocampus might explain higher radiosensitivity of this brain region. Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD, and CAT activities after dose of 5 Gy and to find out if differences in MnSOD activity are caused by changes in its expression. Heads of 4-day-old female rats were irradiated with gamma-rays, using Co-60. Animals were sacrificed 1/24 h after exposure. Hippocampus and cortex tissues were prepared for enzyme activity measurements and Western blot analysis. One hour after exposure, gamma-rays significantly decreased MnSOD activity in both examined brain regions. Twenty-four hours later, MnSOD recovery showed dose and regional dependence. It was weaker at higher doses and in hippocampal region. MnSOD expression changed in the similar manner as MnSOD activity only at lower doses of gamma-rays. In both examined brain regions, gamma radiation significantly decreased CuZnSOD activity and did not change activity of CAT. Our results confirmed that MnSOD plays an important role in different regional radiosensitivity but also showed that depending on dose, radiation affects MnSOD level by utterly different mechanisms. Postradiation changes of CuZnSOD and CAT are not regionally specific and therefore, cannot account for the different radiosensitivity of the hippocampus and cortex.",
journal = "Childs Nervous System",
title = "Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity",
volume = "31",
number = "12",
pages = "2249-2256",
doi = "10.1007/s00381-015-2807-2"
}
Todorović, A., Pejić, S., Stojiljković, V., Gavrilović, L., Popović, N. M., Pavlović, I., Saicic, Z. S.,& Pajović, S. B. (2015). Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity.
Childs Nervous System, 31(12), 2249-2256.
https://doi.org/10.1007/s00381-015-2807-2
Todorović A, Pejić S, Stojiljković V, Gavrilović L, Popović NM, Pavlović I, Saicic ZS, Pajović SB. Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity. Childs Nervous System. 2015;31(12):2249-2256
Todorović Ana, Pejić Snežana, Stojiljković Vesna, Gavrilović Ljubica, Popović Nataša M., Pavlović Ivan, Saicic Zorica S., Pajović Snežana B., "Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity" Childs Nervous System, 31, no. 12 (2015):2249-2256,
https://doi.org/10.1007/s00381-015-2807-2 .
3
2
1

Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia

Gowder, Sivakumar Joghi Thatha; Pejić, Snežana; Todorović, Ana; Stojiljković, Vesna; Pavlović, Ivan; Gavrilović, Ljubica; Popović, Nataša M.; Pajović, Snežana B.

(IntechOpen, 2015)

TY  - CHAP
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8408
AB  - Cancer of the reproductive tract is an important source of morbidity and mortality among women worldwide. Factors affecting endometrial cancer and endometrial hyperplasia are known to be similar. Endometrial hyperplasia is abnormal proliferation of the glands and the stroma resulting in architectural and cytological modifications. Due to hormonal changes, this condition is most common among women who are nearing the menopause or have reached the menopause. Antioxidant system has a role in preventing cancer initiation and promotion. Since the carcinogenesis occurs in several stages, it is likely that the antioxidant defense depends on the type of cell and tissue. The objective of this study was to investigate whether antioxidant enzymes activities and lipid hydroperoxides concentration in patients with endometrial hyperplasia are influenced by the changes in sex hormones level (estradiol, progesterone, FSH, and LH) during the menstrual cycle and in postmenopause. The material we used consisted of blood and endometrial tissue specimens of women diagnosed with endometrial hyperplasia simplex. Patients were divided in groups depending on the phase of the menstrual cycle: follicular phase, luteal phase and postmenopause. The activities of antioxidant enzymes and the lipid hydroperoxides level were compared among the phases to test the differences and a linear regression model was used to evaluate the associations between hormone levels and antioxidant/oxidant variables. In the blood of examined patients, we observed a phase-related changes of LOOH concentrations. Significant negative correlation between FSH concentration and GR activity (r= -0.42, p<0.05) and significant positive correlation between LH and LOOH concentrations (r= 0.038, p<0.05) was found. In hyperplasia simplex tissue we recorded significant phase-related changes of LOOH level as well as of AO enzyme activities. SOD and CAT had similar activity pattern, which was higher in luteal phase and in postmenopause, compared to follicular phase (p<0.05). GPx and GR activities did not show any statistical difference. Also, negative correlation between progesterone and GR activity (r=-0.036, p<0.05) was observed. Hormonal influence on AO system is of importance in gynecological diseases etiology since they may promote cell proliferation but are also used in conservative therapy, especially for hyperplasia simplex. However, the role of ROS production as a risk factor for endometrial hyperplasia still needs to be clarified as well as the role of AO status in response to gonadotropins and sex steroids.
PB  - IntechOpen
PB  - InTech
T2  - Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder
T1  - Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia
IS  - Ch. 9
SP  - 243
EP  - 279
DO  - 10.5772/60853
ER  - 
@article{
editor = "Gowder, Sivakumar Joghi Thatha",
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Pavlović, Ivan and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8408",
abstract = "Cancer of the reproductive tract is an important source of morbidity and mortality among women worldwide. Factors affecting endometrial cancer and endometrial hyperplasia are known to be similar. Endometrial hyperplasia is abnormal proliferation of the glands and the stroma resulting in architectural and cytological modifications. Due to hormonal changes, this condition is most common among women who are nearing the menopause or have reached the menopause. Antioxidant system has a role in preventing cancer initiation and promotion. Since the carcinogenesis occurs in several stages, it is likely that the antioxidant defense depends on the type of cell and tissue. The objective of this study was to investigate whether antioxidant enzymes activities and lipid hydroperoxides concentration in patients with endometrial hyperplasia are influenced by the changes in sex hormones level (estradiol, progesterone, FSH, and LH) during the menstrual cycle and in postmenopause. The material we used consisted of blood and endometrial tissue specimens of women diagnosed with endometrial hyperplasia simplex. Patients were divided in groups depending on the phase of the menstrual cycle: follicular phase, luteal phase and postmenopause. The activities of antioxidant enzymes and the lipid hydroperoxides level were compared among the phases to test the differences and a linear regression model was used to evaluate the associations between hormone levels and antioxidant/oxidant variables. In the blood of examined patients, we observed a phase-related changes of LOOH concentrations. Significant negative correlation between FSH concentration and GR activity (r= -0.42, p<0.05) and significant positive correlation between LH and LOOH concentrations (r= 0.038, p<0.05) was found. In hyperplasia simplex tissue we recorded significant phase-related changes of LOOH level as well as of AO enzyme activities. SOD and CAT had similar activity pattern, which was higher in luteal phase and in postmenopause, compared to follicular phase (p<0.05). GPx and GR activities did not show any statistical difference. Also, negative correlation between progesterone and GR activity (r=-0.036, p<0.05) was observed. Hormonal influence on AO system is of importance in gynecological diseases etiology since they may promote cell proliferation but are also used in conservative therapy, especially for hyperplasia simplex. However, the role of ROS production as a risk factor for endometrial hyperplasia still needs to be clarified as well as the role of AO status in response to gonadotropins and sex steroids.",
publisher = "IntechOpen, InTech",
journal = "Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder",
title = "Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia",
number = "Ch. 9",
pages = "243-279",
doi = "10.5772/60853"
}
Gowder, S. J. T., Pejić, S., Todorović, A., Stojiljković, V., Pavlović, I., Gavrilović, L., Popović, N. M.,& Pajović, S. B. (2015). Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia.
Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder
InTech.(Ch. 9), 243-279.
https://doi.org/10.5772/60853
Gowder SJT, Pejić S, Todorović A, Stojiljković V, Pavlović I, Gavrilović L, Popović NM, Pajović SB. Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia. Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder. 2015;(Ch. 9):243-279
Gowder Sivakumar Joghi Thatha, Pejić Snežana, Todorović Ana, Stojiljković Vesna, Pavlović Ivan, Gavrilović Ljubica, Popović Nataša M., Pajović Snežana B., "Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia" Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder, no. Ch. 9 (2015):243-279,
https://doi.org/10.5772/60853 .
4

Antioxidant status in women with uterine leiomyoma: relation with sex hormones

Pejić, Snežana; Todorović, Ana; Stojiljković, Vesna; Gavrilović, Ljubica; Popović, Nataša M.; Pajović, Snežana B.

(2015)

TY  - JOUR
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/751
AB  - Uterine leiomyomas are benign soft-tissues tumors that arise from uterine smooth muscle tissue. Etiopathogenesis of leiomyomas is not well understood. We aimed to examine whether antioxidant enzyme activities and lipid hydroperoxides level in patients with leiomyoma are influenced by changes in sex hormones and gonadotropins (estradiol (E2), progesterone, FSH, and LH) during menstrual cycle and in postmenopause. The material consisted of blood and uterine tissue specimens. Hormone concentrations were determined and assays for superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities and lipid hydroperoxides concentration were performed. In blood of examined women, a significant difference in catalase, glutathione peroxidase and glutathione reductase activity was recorded among the phases. There was also a positive correlation between the estradiol/progesterone concentration and the catalase activity. Progesterone negatively correlated with lipid hydroperoxides level. In myoma tissue, we recorded a phase-related difference in lipid hydroperoxides level and activities of superoxide dismutase, glutathione peroxidase activities, and glutathione reductase. Negative correlation was observed between FSH and glutathione peroxidase. The results suggest that antioxidant status in patients with uterine leiomyoma is influenced by the changes in sex hormones during the menstrual cycle and in postmenopause, indicating a role of the observed relationship in the leiomyoma etiology.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Antioxidant status in women with uterine leiomyoma: relation with sex hormones
VL  - 87
IS  - 3
SP  - 1771
EP  - 1782
DO  - 10.1590/0001-3765201520130416
ER  - 
@article{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/751",
abstract = "Uterine leiomyomas are benign soft-tissues tumors that arise from uterine smooth muscle tissue. Etiopathogenesis of leiomyomas is not well understood. We aimed to examine whether antioxidant enzyme activities and lipid hydroperoxides level in patients with leiomyoma are influenced by changes in sex hormones and gonadotropins (estradiol (E2), progesterone, FSH, and LH) during menstrual cycle and in postmenopause. The material consisted of blood and uterine tissue specimens. Hormone concentrations were determined and assays for superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities and lipid hydroperoxides concentration were performed. In blood of examined women, a significant difference in catalase, glutathione peroxidase and glutathione reductase activity was recorded among the phases. There was also a positive correlation between the estradiol/progesterone concentration and the catalase activity. Progesterone negatively correlated with lipid hydroperoxides level. In myoma tissue, we recorded a phase-related difference in lipid hydroperoxides level and activities of superoxide dismutase, glutathione peroxidase activities, and glutathione reductase. Negative correlation was observed between FSH and glutathione peroxidase. The results suggest that antioxidant status in patients with uterine leiomyoma is influenced by the changes in sex hormones during the menstrual cycle and in postmenopause, indicating a role of the observed relationship in the leiomyoma etiology.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Antioxidant status in women with uterine leiomyoma: relation with sex hormones",
volume = "87",
number = "3",
pages = "1771-1782",
doi = "10.1590/0001-3765201520130416"
}
Pejić, S., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M.,& Pajović, S. B. (2015). Antioxidant status in women with uterine leiomyoma: relation with sex hormones.
Anais de Academia Brasileira de Ciencias, 87(3), 1771-1782.
https://doi.org/10.1590/0001-3765201520130416
Pejić S, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Pajović SB. Antioxidant status in women with uterine leiomyoma: relation with sex hormones. Anais de Academia Brasileira de Ciencias. 2015;87(3):1771-1782
Pejić Snežana, Todorović Ana, Stojiljković Vesna, Gavrilović Ljubica, Popović Nataša M., Pajović Snežana B., "Antioxidant status in women with uterine leiomyoma: relation with sex hormones" Anais de Academia Brasileira de Ciencias, 87, no. 3 (2015):1771-1782,
https://doi.org/10.1590/0001-3765201520130416 .
5
3
3

Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons

Keta, Otilija D.; Todorović, Danijela V.; Popović, Nataša M.; Korićanac, Lela; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2014)

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Popović, Nataša M.
AU  - Korićanac, Lela
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5447
AB  - Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.
T2  - Archives of Medical Science
T1  - Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons
VL  - 10
IS  - 3
SP  - 578
EP  - 586
DO  - 10.5114/aoms.2014.43751
ER  - 
@article{
author = "Keta, Otilija D. and Todorović, Danijela V. and Popović, Nataša M. and Korićanac, Lela and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5447",
abstract = "Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.",
journal = "Archives of Medical Science",
title = "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons",
volume = "10",
number = "3",
pages = "578-586",
doi = "10.5114/aoms.2014.43751"
}
Keta, O. D., Todorović, D. V., Popović, N. M., Korićanac, L., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A. (2014). Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons.
Archives of Medical Science, 10(3), 578-586.
https://doi.org/10.5114/aoms.2014.43751
Keta OD, Todorović DV, Popović NM, Korićanac L, Cuttone G, Petrović IM, Ristić-Fira A. Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. Archives of Medical Science. 2014;10(3):578-586
Keta Otilija D., Todorović Danijela V., Popović Nataša M., Korićanac Lela, Cuttone Giacomo, Petrović Ivan M., Ristić-Fira Aleksandra, "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons" Archives of Medical Science, 10, no. 3 (2014):578-586,
https://doi.org/10.5114/aoms.2014.43751 .
8
8
10

Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats

Gavrilović, Ljubica; Stojiljković, Vesna; Kasapović, Jelena; Popović, Nataša M.; Pajović, Snežana B.; Dronjak, Slađana

(2013)

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Kasapović, Jelena
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Dronjak, Slađana
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5703
AB  - Chronic isolation of adult animals represents a form of psychological stress that produces sympatho-adrenomedullar activation. Exercise training acts as an important modulator of sympatho-adrenomedullary system. This study aimed to investigate physical exercise-related changes in gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase, dopamine-beta-hydroxylase and phenylethanolamine N-methyltransferase) and cyclic adenosine monophosphate response element-binding (CREB) in the adrenal medulla, concentrations of catecholamines and corticosterone (CORT) in the plasma and the weight of adrenal glands of chronically psychosocially stressed adult rats exposed daily to 20 min treadmill running for 12 weeks, Also, we examined how additional acute immobilization stress changes the mentioned parameters. Treadmill running did not result in modulation of gene expression of catecholamine synthesizing enzymes and it decreased the level of CREB mRNA in the adrenal medulla of chronically psychosocially stressed adult rats. The potentially negative physiological adaptations after treadmill running were recorded as increased concentrations of catecholamines and decreased morning CORT concentration in the plasma, as well as the adrenal gland hypertrophy of chronically psychosocially stressed rats. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. Treadmill exercise does not change the activity of sympatho-adrenomedullary system of chronically psychosocially stressed rats.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats
VL  - 85
IS  - 3
SP  - 999
EP  - 1012
DO  - 10.1590/S0001-37652013005000041
ER  - 
@article{
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Kasapović, Jelena and Popović, Nataša M. and Pajović, Snežana B. and Dronjak, Slađana",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5703",
abstract = "Chronic isolation of adult animals represents a form of psychological stress that produces sympatho-adrenomedullar activation. Exercise training acts as an important modulator of sympatho-adrenomedullary system. This study aimed to investigate physical exercise-related changes in gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase, dopamine-beta-hydroxylase and phenylethanolamine N-methyltransferase) and cyclic adenosine monophosphate response element-binding (CREB) in the adrenal medulla, concentrations of catecholamines and corticosterone (CORT) in the plasma and the weight of adrenal glands of chronically psychosocially stressed adult rats exposed daily to 20 min treadmill running for 12 weeks, Also, we examined how additional acute immobilization stress changes the mentioned parameters. Treadmill running did not result in modulation of gene expression of catecholamine synthesizing enzymes and it decreased the level of CREB mRNA in the adrenal medulla of chronically psychosocially stressed adult rats. The potentially negative physiological adaptations after treadmill running were recorded as increased concentrations of catecholamines and decreased morning CORT concentration in the plasma, as well as the adrenal gland hypertrophy of chronically psychosocially stressed rats. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. Treadmill exercise does not change the activity of sympatho-adrenomedullary system of chronically psychosocially stressed rats.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats",
volume = "85",
number = "3",
pages = "999-1012",
doi = "10.1590/S0001-37652013005000041"
}
Gavrilović, L., Stojiljković, V., Kasapović, J., Popović, N. M., Pajović, S. B.,& Dronjak, S. (2013). Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats.
Anais de Academia Brasileira de Ciencias, 85(3), 999-1012.
https://doi.org/10.1590/S0001-37652013005000041
Gavrilović L, Stojiljković V, Kasapović J, Popović NM, Pajović SB, Dronjak S. Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats. Anais de Academia Brasileira de Ciencias. 2013;85(3):999-1012
Gavrilović Ljubica, Stojiljković Vesna, Kasapović Jelena, Popović Nataša M., Pajović Snežana B., Dronjak Slađana, "Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats" Anais de Academia Brasileira de Ciencias, 85, no. 3 (2013):999-1012,
https://doi.org/10.1590/S0001-37652013005000041 .
8
8
9

Antioxidant enzymes in women with endometrial polyps: relation with sex hormones

Pejić, Snežana; Kasapović, Jelena; Todorović, Ana; Stojiljković, Vesna; Gavrilović, Ljubica; Popović, Nataša M.; Pajović, Snežana B.

(2013)

TY  - JOUR
AU  - Pejić, Snežana
AU  - Kasapović, Jelena
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5705
AB  - Objective: To investigate whether antioxidant enzyme activities (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase) and lipid hydroperoxide levels in patients with endometrial polyps are influenced by the changes in sex hormones (estradiol, progesterone, FSH, and LH) during the menstrual cycle and in postmenopause. Study design: The material consisted of blood and endometrial tissue specimens from women diagnosed with endometrial polyps. Patients were divided into groups depending on the phase of the menstrual cycle - follicular or luteal - and the postmenopause. The activities of antioxidant enzymes and the lipid hydroperoxide levels were compared among the phases and a linear regression model was used to evaluate the associations between hormones and antioxidant/oxidant variables. Results: In the blood of examined women, a significant difference in superoxide dismutase activity and lipid hydroperoxide levels was recorded among the phases. There was also a positive correlation between the estradiol concentration and superoxide dismutase. In polyp tissue, we recorded a phase-related difference in superoxide dismutase and glutathione peroxidase activities as well as in the lipid hydroperoxide levels. A negative correlation was observed between FSH/LH and glutathione peroxidase, and between LH and superoxide dismutase. Conclusion: Antioxidant enzymes and lipid hydroperoxide levels in patients with endometrial polyps are influenced by the changes in sex hormones during the menstrual cycle and after the menopause, pointing to a role of the observed relationship in polyp etiology. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
T2  - European Journal of Obstetrics Gynecology and Reproductive Biology
T1  - Antioxidant enzymes in women with endometrial polyps: relation with sex hormones
VL  - 170
IS  - 1
SP  - 241
EP  - 246
DO  - 10.1016/j.ejogrb.2013.06.024
ER  - 
@article{
author = "Pejić, Snežana and Kasapović, Jelena and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5705",
abstract = "Objective: To investigate whether antioxidant enzyme activities (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase) and lipid hydroperoxide levels in patients with endometrial polyps are influenced by the changes in sex hormones (estradiol, progesterone, FSH, and LH) during the menstrual cycle and in postmenopause. Study design: The material consisted of blood and endometrial tissue specimens from women diagnosed with endometrial polyps. Patients were divided into groups depending on the phase of the menstrual cycle - follicular or luteal - and the postmenopause. The activities of antioxidant enzymes and the lipid hydroperoxide levels were compared among the phases and a linear regression model was used to evaluate the associations between hormones and antioxidant/oxidant variables. Results: In the blood of examined women, a significant difference in superoxide dismutase activity and lipid hydroperoxide levels was recorded among the phases. There was also a positive correlation between the estradiol concentration and superoxide dismutase. In polyp tissue, we recorded a phase-related difference in superoxide dismutase and glutathione peroxidase activities as well as in the lipid hydroperoxide levels. A negative correlation was observed between FSH/LH and glutathione peroxidase, and between LH and superoxide dismutase. Conclusion: Antioxidant enzymes and lipid hydroperoxide levels in patients with endometrial polyps are influenced by the changes in sex hormones during the menstrual cycle and after the menopause, pointing to a role of the observed relationship in polyp etiology. (C) 2013 Elsevier Ireland Ltd. All rights reserved.",
journal = "European Journal of Obstetrics Gynecology and Reproductive Biology",
title = "Antioxidant enzymes in women with endometrial polyps: relation with sex hormones",
volume = "170",
number = "1",
pages = "241-246",
doi = "10.1016/j.ejogrb.2013.06.024"
}
Pejić, S., Kasapović, J., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M.,& Pajović, S. B. (2013). Antioxidant enzymes in women with endometrial polyps: relation with sex hormones.
European Journal of Obstetrics Gynecology and Reproductive Biology, 170(1), 241-246.
https://doi.org/10.1016/j.ejogrb.2013.06.024
Pejić S, Kasapović J, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Pajović SB. Antioxidant enzymes in women with endometrial polyps: relation with sex hormones. European Journal of Obstetrics Gynecology and Reproductive Biology. 2013;170(1):241-246
Pejić Snežana, Kasapović Jelena, Todorović Ana, Stojiljković Vesna, Gavrilović Ljubica, Popović Nataša M., Pajović Snežana B., "Antioxidant enzymes in women with endometrial polyps: relation with sex hormones" European Journal of Obstetrics Gynecology and Reproductive Biology, 170, no. 1 (2013):241-246,
https://doi.org/10.1016/j.ejogrb.2013.06.024 .
6
6
7

Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging

Kasapović, Jelena; Stojiljković, Vesna; Gavrilović, Ljubica; Popović, Nataša M.; Milićević, Zorka T.

(2012)

TY  - JOUR
AU  - Kasapović, Jelena
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Milićević, Zorka T.
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4959
AB  - Reactive oxygen species (ROS) are independently recognized to play a significant role in radiation-induced damage on healthy tissue and in aging process. However, an age-related alteration of antioxidant (AO) system in radiation response in humans is poorly investigated. The aim of this paper was to evaluate the irradiation effects on the activities and expression of AO system in the blood of healthy women during aging. Blood samples were irradiated with curative and palliative doses of 2Gy or 9Gy gamma-rays. AO capacity for detoxification of O-2 center dot(-) and H2O2 in response to 2Gy gamma-irradiation decreases in women above 58 years, while in response to 9Gy shows signs of weakening after 45 years of age. Due to reduction of AO capacity during aging, cytotoxic effects of curative and palliative doses of irradiation, mediated by ROS, may significantly increase in older subjects, while removal of H2O2 excess could reduce them.
T2  - Scientific World Journal
T1  - Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging
DO  - 10.1100/2012/982594
ER  - 
@article{
author = "Kasapović, Jelena and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Milićević, Zorka T.",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4959",
abstract = "Reactive oxygen species (ROS) are independently recognized to play a significant role in radiation-induced damage on healthy tissue and in aging process. However, an age-related alteration of antioxidant (AO) system in radiation response in humans is poorly investigated. The aim of this paper was to evaluate the irradiation effects on the activities and expression of AO system in the blood of healthy women during aging. Blood samples were irradiated with curative and palliative doses of 2Gy or 9Gy gamma-rays. AO capacity for detoxification of O-2 center dot(-) and H2O2 in response to 2Gy gamma-irradiation decreases in women above 58 years, while in response to 9Gy shows signs of weakening after 45 years of age. Due to reduction of AO capacity during aging, cytotoxic effects of curative and palliative doses of irradiation, mediated by ROS, may significantly increase in older subjects, while removal of H2O2 excess could reduce them.",
journal = "Scientific World Journal",
title = "Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging",
doi = "10.1100/2012/982594"
}
Kasapović, J., Stojiljković, V., Gavrilović, L., Popović, N. M.,& Milićević, Z. T. (2012). Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging.
Scientific World Journal.
https://doi.org/10.1100/2012/982594
Kasapović J, Stojiljković V, Gavrilović L, Popović NM, Milićević ZT. Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging. Scientific World Journal. 2012;
Kasapović Jelena, Stojiljković Vesna, Gavrilović Ljubica, Popović Nataša M., Milićević Zorka T., "Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging" Scientific World Journal (2012),
https://doi.org/10.1100/2012/982594 .
1
1

Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae

Popović, Nataša M.; Ruždijić, Sabera; Kanazir, Dusan T.; Niciforovic, Ana; Adžić, Miroslav; Paraskevopoulou, Elissavet; Pantelidou, Constantia; Radoičić, Marija B.; Demonacos, Constantinos; Krstic-Demonacos, Marija

(2010)

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Ruždijić, Sabera
AU  - Kanazir, Dusan T.
AU  - Niciforovic, Ana
AU  - Adžić, Miroslav
AU  - Paraskevopoulou, Elissavet
AU  - Pantelidou, Constantia
AU  - Radoičić, Marija B.
AU  - Demonacos, Constantinos
AU  - Krstic-Demonacos, Marija
PY  - 2010
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3999
AB  - The glucocorticoid receptor (GR) signal transduction and transcriptional regulation are efficiently recapitulated when GR is expressed in Saccharomyces cerevisiae. In this report we demonstrate that the in vivo GR phosphorylation pattern, hormone dependency and interdependency of phosphorylation events were similar in yeast and mammalian cells. GR phosphorylation at S246 exhibited inhibitory effect on S224 and S232 phosphorylation, suggesting the conservation of molecular mechanisms that control this interdependence between yeast and mammalian cells. To assess the effects of GR phosphorylation the mutated GR derivatives T171A, S224A, S232A, S246A were overexpressed and their transcriptional activity was analysed. These receptor derivatives displayed significant hormone inducible transcription when overexpressed in S. cerevisiae. We have established an inducible methionine expression system, which allows the close regulation of the receptor protein levels to analyse the dependence of GR function on its phosphorylation and protein abundance. Using this system we observed that GR S246A mutation increased its activity across all of the GR concentrations tested. The activity of the S224A and S246A mutants was mostly independent of GR protein levels, whereas the WT, T171A and S232A mediated transcription diminished with declining GR protein levels. Our results suggest that GR phosphorylation at specific residues affects its transcriptional functions in a site selective manner and these effects were directly linked to GR dosage. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
T2  - Steroids
T1  - Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae
VL  - 75
IS  - 6
SP  - 457
EP  - 465
DO  - 10.1016/j.steroids.2010.03.001
ER  - 
@article{
author = "Popović, Nataša M. and Ruždijić, Sabera and Kanazir, Dusan T. and Niciforovic, Ana and Adžić, Miroslav and Paraskevopoulou, Elissavet and Pantelidou, Constantia and Radoičić, Marija B. and Demonacos, Constantinos and Krstic-Demonacos, Marija",
year = "2010",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3999",
abstract = "The glucocorticoid receptor (GR) signal transduction and transcriptional regulation are efficiently recapitulated when GR is expressed in Saccharomyces cerevisiae. In this report we demonstrate that the in vivo GR phosphorylation pattern, hormone dependency and interdependency of phosphorylation events were similar in yeast and mammalian cells. GR phosphorylation at S246 exhibited inhibitory effect on S224 and S232 phosphorylation, suggesting the conservation of molecular mechanisms that control this interdependence between yeast and mammalian cells. To assess the effects of GR phosphorylation the mutated GR derivatives T171A, S224A, S232A, S246A were overexpressed and their transcriptional activity was analysed. These receptor derivatives displayed significant hormone inducible transcription when overexpressed in S. cerevisiae. We have established an inducible methionine expression system, which allows the close regulation of the receptor protein levels to analyse the dependence of GR function on its phosphorylation and protein abundance. Using this system we observed that GR S246A mutation increased its activity across all of the GR concentrations tested. The activity of the S224A and S246A mutants was mostly independent of GR protein levels, whereas the WT, T171A and S232A mediated transcription diminished with declining GR protein levels. Our results suggest that GR phosphorylation at specific residues affects its transcriptional functions in a site selective manner and these effects were directly linked to GR dosage. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.",
journal = "Steroids",
title = "Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae",
volume = "75",
number = "6",
pages = "457-465",
doi = "10.1016/j.steroids.2010.03.001"
}
Popović, N. M., Ruždijić, S., Kanazir, D. T., Niciforovic, A., Adžić, M., Paraskevopoulou, E., Pantelidou, C., Radoičić, M. B., Demonacos, C.,& Krstic-Demonacos, M. (2010). Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae.
Steroids, 75(6), 457-465.
https://doi.org/10.1016/j.steroids.2010.03.001
Popović NM, Ruždijić S, Kanazir DT, Niciforovic A, Adžić M, Paraskevopoulou E, Pantelidou C, Radoičić MB, Demonacos C, Krstic-Demonacos M. Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae. Steroids. 2010;75(6):457-465
Popović Nataša M., Ruždijić Sabera, Kanazir Dusan T., Niciforovic Ana, Adžić Miroslav, Paraskevopoulou Elissavet, Pantelidou Constantia, Radoičić Marija B., Demonacos Constantinos, Krstic-Demonacos Marija, "Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae" Steroids, 75, no. 6 (2010):457-465,
https://doi.org/10.1016/j.steroids.2010.03.001 .
3
3
3

Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats

Popović, Nataša M.; Pajović, Snežana B.

(2010)

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2010
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4048
AB  - In the present study we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex, as well as the serum corticosterone and glucose level of mature male Wistar rats subjected to three different treatments: animals subjected to chronic restraint-stress, animals injected with lithium (Li) and chronically stressed rats treated with Li. Under all three conditions we observed hypertrophy of whole adrenals, as well as the adrenal cortices. Chronic restraint stress, solely or in combination with Li treatment, significantly elevated the corticosterone level, but did not change the blood glucose level. Animals treated only with Li exhibited an elevated serum corticosterone level and blood glucose level. The aim of our study was to investigate the modulation of the chronic stress-induced effect on the blood glucose level by lithium, as a possible mechanism of avoiding the damage caused by chronic stress. Our results showed that lithium is an agent of choice which may help to reduce stress-elevated corticosterone and replenish exhausted glucose storages in an organism.
T2  - Archives of biological sciences
T1  - Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats
VL  - 62
IS  - 2
SP  - 289
EP  - 295
DO  - 10.2298/ABS1002289P
ER  - 
@article{
author = "Popović, Nataša M. and Pajović, Snežana B.",
year = "2010",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4048",
abstract = "In the present study we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex, as well as the serum corticosterone and glucose level of mature male Wistar rats subjected to three different treatments: animals subjected to chronic restraint-stress, animals injected with lithium (Li) and chronically stressed rats treated with Li. Under all three conditions we observed hypertrophy of whole adrenals, as well as the adrenal cortices. Chronic restraint stress, solely or in combination with Li treatment, significantly elevated the corticosterone level, but did not change the blood glucose level. Animals treated only with Li exhibited an elevated serum corticosterone level and blood glucose level. The aim of our study was to investigate the modulation of the chronic stress-induced effect on the blood glucose level by lithium, as a possible mechanism of avoiding the damage caused by chronic stress. Our results showed that lithium is an agent of choice which may help to reduce stress-elevated corticosterone and replenish exhausted glucose storages in an organism.",
journal = "Archives of biological sciences",
title = "Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats",
volume = "62",
number = "2",
pages = "289-295",
doi = "10.2298/ABS1002289P"
}
Popović, N. M.,& Pajović, S. B. (2010). Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats.
Archives of biological sciences, 62(2), 289-295.
https://doi.org/10.2298/ABS1002289P
Popović NM, Pajović SB. Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats. Archives of biological sciences. 2010;62(2):289-295
Popović Nataša M., Pajović Snežana B., "Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats" Archives of biological sciences, 62, no. 2 (2010):289-295,
https://doi.org/10.2298/ABS1002289P .
3
3
5

Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays

Popović, Nataša M.; Niciforovic, Ana; Adžić, Miroslav; Radoičić, Marija B.; Demonacos, Constantinos; Krstic-Demonacos, Marija

(2009)

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Niciforovic, Ana
AU  - Adžić, Miroslav
AU  - Radoičić, Marija B.
AU  - Demonacos, Constantinos
AU  - Krstic-Demonacos, Marija
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3667
AB  - The glucocorticoid receptor (GR) protein is a cytosolic ligand-dependent transcription factor with numerous functions regulated by post-translational modifications, including phosphorylation/dephosphorylation. Among the functions most extensively affected by GR phosphorylation are the modulation of its transcriptional activity, alterations in its interaction pattern with cofactors, nuclear translocation and selective gene transactivation. Intensive analysis of the intracellular distribution of GR phosphoisoforms and their interaction with proteins of other cellular signalling networks required the use of [gamma-(32)P]ATP as a phosphate donor, and special laboratory protection measures to avoid external irradiation and contamination. In the present study, simple and easy-to-use non-radioactive protein mobility shift assays (NMS assays) were developed using one- and/or two-dimensional gel electrophoresis based on differences in the pI and molecular mass of GR phosphoisoforms. The GR isoforms were immunodetected with specific monoclonal or polyclonal anti-GR antibodies by Western blot in three diverse systems, namely yeast BJ2168 cells expressing wild-type rat GR, rat hepatoma GRH2 cells grown in culture and brain tissue from Wistar rat experimental animals. The results obtained using the NMS assay were similar to previous results obtained with the [gamma-(32)P] ATP standard assay.
T2  - Journal of the Serbian Chemical Society
T1  - Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays
VL  - 74
IS  - 3
SP  - 237
EP  - 244
DO  - 10.2298/JSC0903237P
ER  - 
@article{
author = "Popović, Nataša M. and Niciforovic, Ana and Adžić, Miroslav and Radoičić, Marija B. and Demonacos, Constantinos and Krstic-Demonacos, Marija",
year = "2009",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3667",
abstract = "The glucocorticoid receptor (GR) protein is a cytosolic ligand-dependent transcription factor with numerous functions regulated by post-translational modifications, including phosphorylation/dephosphorylation. Among the functions most extensively affected by GR phosphorylation are the modulation of its transcriptional activity, alterations in its interaction pattern with cofactors, nuclear translocation and selective gene transactivation. Intensive analysis of the intracellular distribution of GR phosphoisoforms and their interaction with proteins of other cellular signalling networks required the use of [gamma-(32)P]ATP as a phosphate donor, and special laboratory protection measures to avoid external irradiation and contamination. In the present study, simple and easy-to-use non-radioactive protein mobility shift assays (NMS assays) were developed using one- and/or two-dimensional gel electrophoresis based on differences in the pI and molecular mass of GR phosphoisoforms. The GR isoforms were immunodetected with specific monoclonal or polyclonal anti-GR antibodies by Western blot in three diverse systems, namely yeast BJ2168 cells expressing wild-type rat GR, rat hepatoma GRH2 cells grown in culture and brain tissue from Wistar rat experimental animals. The results obtained using the NMS assay were similar to previous results obtained with the [gamma-(32)P] ATP standard assay.",
journal = "Journal of the Serbian Chemical Society",
title = "Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays",
volume = "74",
number = "3",
pages = "237-244",
doi = "10.2298/JSC0903237P"
}
Popović, N. M., Niciforovic, A., Adžić, M., Radoičić, M. B., Demonacos, C.,& Krstic-Demonacos, M. (2009). Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays.
Journal of the Serbian Chemical Society, 74(3), 237-244.
https://doi.org/10.2298/JSC0903237P
Popović NM, Niciforovic A, Adžić M, Radoičić MB, Demonacos C, Krstic-Demonacos M. Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays. Journal of the Serbian Chemical Society. 2009;74(3):237-244
Popović Nataša M., Niciforovic Ana, Adžić Miroslav, Radoičić Marija B., Demonacos Constantinos, Krstic-Demonacos Marija, "Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays" Journal of the Serbian Chemical Society, 74, no. 3 (2009):237-244,
https://doi.org/10.2298/JSC0903237P .