TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Mančić, Lidija
AU  - Vuković, Marina
AU  - Stojanović, Zoran S.
AU  - Nikolić, Marko G.
AU  - Škapin, Srečo Davor
AU  - Jovanović, Sonja
AU  - Veselinović, Ljiljana M.
AU  - Uskoković, Vuk
AU  - Lazić, Snežana
AU  - Marković, Smilja
AU  - Lazarević, Miloš M.
AU  - Uskoković, Dragan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8625
AB  - Taking advantage of the flexibility of the apatite structure, nano- and micro-particles of hydroxyapatite (HAp) were doped with different combinations of rare earth ions (RE3+ = Gd, Eu, Yb, Tm) to achieve a synergy among their magnetic and optical properties and to enable their application in preventive medicine, particularly diagnostics based on multimodal imaging. All powders were synthesized through hydrothermal processing at T ≤ 200 °C. An X-ray powder diffraction analysis showed that all powders crystallized in P63/m space group of the hexagonal crystal structure. The refined unit-cell parameters reflected a decrease in the unit cell volume as a result of the partial substitution of Ca2+ with smaller RE3+ ions at both cation positions. The FTIR analysis additionally suggested that a synergy may exist solely in the triply doped system, where the lattice symmetry and vibration modes become more coherent than in the singly or doubly doped systems. HAp:RE3+ optical characterization revealed a change in the energy band gap and the appearance of a weak blue luminescence (λex = 370 nm) due to an increased concentration of defects. The “up”- and the “down”-conversion spectra of HAp:Gd/Yb/Tm and HAp:Gd/Eu powders showed characteristic transitions of Tm3+ and Eu3+, respectively. Furthermore, in contrast to diamagnetic HAp, all HAp:RE3+ powders exhibited paramagnetic behavior. Cell viability tests of HAp:Gd/Yb/Tm and HAp:Gd/Eu powders in human dental pulp stem cell cultures indicated their good biocompatibility. © 2019, The Author(s).
T2  - Scientific Reports
T1  - Rare-earth (Gd3+,Yb3+/Tm3+, Eu3+) co-doped hydroxyapatite as magnetic, up-conversion and down-conversion materials for multimodal imaging
VL  - 9
IS  - 1
SP  - 16305
DO  - 10.1038/s41598-019-52885-0
ER  - 

@article{
author = "Ignjatović, Nenad L. and Mančić, Lidija and Vuković, Marina and Stojanović, Zoran S. and Nikolić, Marko G. and Škapin, Srečo Davor and Jovanović, Sonja and Veselinović, Ljiljana M. and Uskoković, Vuk and Lazić, Snežana and Marković, Smilja and Lazarević, Miloš M. and Uskoković, Dragan",
year = "2019",
abstract = "Taking advantage of the flexibility of the apatite structure, nano- and micro-particles of hydroxyapatite (HAp) were doped with different combinations of rare earth ions (RE3+ = Gd, Eu, Yb, Tm) to achieve a synergy among their magnetic and optical properties and to enable their application in preventive medicine, particularly diagnostics based on multimodal imaging. All powders were synthesized through hydrothermal processing at T ≤ 200 °C. An X-ray powder diffraction analysis showed that all powders crystallized in P63/m space group of the hexagonal crystal structure. The refined unit-cell parameters reflected a decrease in the unit cell volume as a result of the partial substitution of Ca2+ with smaller RE3+ ions at both cation positions. The FTIR analysis additionally suggested that a synergy may exist solely in the triply doped system, where the lattice symmetry and vibration modes become more coherent than in the singly or doubly doped systems. HAp:RE3+ optical characterization revealed a change in the energy band gap and the appearance of a weak blue luminescence (λex = 370 nm) due to an increased concentration of defects. The “up”- and the “down”-conversion spectra of HAp:Gd/Yb/Tm and HAp:Gd/Eu powders showed characteristic transitions of Tm3+ and Eu3+, respectively. Furthermore, in contrast to diamagnetic HAp, all HAp:RE3+ powders exhibited paramagnetic behavior. Cell viability tests of HAp:Gd/Yb/Tm and HAp:Gd/Eu powders in human dental pulp stem cell cultures indicated their good biocompatibility. © 2019, The Author(s).",
journal = "Scientific Reports",
title = "Rare-earth (Gd3+,Yb3+/Tm3+, Eu3+) co-doped hydroxyapatite as magnetic, up-conversion and down-conversion materials for multimodal imaging",
volume = "9",
number = "1",
pages = "16305",
doi = "10.1038/s41598-019-52885-0"
}

Ignjatović, N. L., Mančić, L., Vuković, M., Stojanović, Z. S., Nikolić, M. G., Škapin, S. D., Jovanović, S., Veselinović, L. M., Uskoković, V., Lazić, S., Marković, S., Lazarević, M. M.,& Uskoković, D.. (2019). Rare-earth (Gd3+,Yb3+/Tm3+, Eu3+) co-doped hydroxyapatite as magnetic, up-conversion and down-conversion materials for multimodal imaging. in Scientific Reports, 9(1), 16305.
https://doi.org/10.1038/s41598-019-52885-0

Ignjatović NL, Mančić L, Vuković M, Stojanović ZS, Nikolić MG, Škapin SD, Jovanović S, Veselinović LM, Uskoković V, Lazić S, Marković S, Lazarević MM, Uskoković D. Rare-earth (Gd3+,Yb3+/Tm3+, Eu3+) co-doped hydroxyapatite as magnetic, up-conversion and down-conversion materials for multimodal imaging. in Scientific Reports. 2019;9(1):16305.
doi:10.1038/s41598-019-52885-0 .

Ignjatović, Nenad L., Mančić, Lidija, Vuković, Marina, Stojanović, Zoran S., Nikolić, Marko G., Škapin, Srečo Davor, Jovanović, Sonja, Veselinović, Ljiljana M., Uskoković, Vuk, Lazić, Snežana, Marković, Smilja, Lazarević, Miloš M., Uskoković, Dragan, "Rare-earth (Gd3+,Yb3+/Tm3+, Eu3+) co-doped hydroxyapatite as magnetic, up-conversion and down-conversion materials for multimodal imaging" in Scientific Reports, 9, no. 1 (2019):16305,
https://doi.org/10.1038/s41598-019-52885-0 . .

TY  - JOUR
AU  - Eljabo, Najib
AU  - Nikolić, Nađa
AU  - Čarkić, Jelena
AU  - Jelovac, Drago B.
AU  - Lazarević, Miloš M.
AU  - Tanić, Nasta
AU  - Milašin, Jelena
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0901502718300389
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7924
AB  - Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers. c-Myc and HER2 genes were quantified by means of real-time PCR (qPCR), and p14 and p16 methylation status was determined by methylation-specific PCR (MSP PCR). All tissues examined exhibited molecular alterations in various proportions. Tumour tissues, as expected, showed the highest prevalence of alterations, while oral mucosa showed the lowest. Multiple alterations (co-alterations) in tumours and tumour margins were significantly more frequent than in unaffected oral mucosa (P < 0.001 and P = 0.027, respectively). HER2 amplification in margin tissue (P < 0.001) and swabs (P = 0.013), as well as the existence of three co-alterations in margins (P = 0.001) and macroscopically unaffected oral mucosa (P < 0.001) were correlated with shorter disease-specific survival.
T2  - International Journal of Oral and Maxillofacial Surgery
T1  - Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer
VL  - 47
IS  - 8
SP  - 976
EP  - 982
DO  - 10.1016/j.ijom.2018.01.020
ER  - 

@article{
author = "Eljabo, Najib and Nikolić, Nađa and Čarkić, Jelena and Jelovac, Drago B. and Lazarević, Miloš M. and Tanić, Nasta and Milašin, Jelena",
year = "2018",
abstract = "Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers. c-Myc and HER2 genes were quantified by means of real-time PCR (qPCR), and p14 and p16 methylation status was determined by methylation-specific PCR (MSP PCR). All tissues examined exhibited molecular alterations in various proportions. Tumour tissues, as expected, showed the highest prevalence of alterations, while oral mucosa showed the lowest. Multiple alterations (co-alterations) in tumours and tumour margins were significantly more frequent than in unaffected oral mucosa (P < 0.001 and P = 0.027, respectively). HER2 amplification in margin tissue (P < 0.001) and swabs (P = 0.013), as well as the existence of three co-alterations in margins (P = 0.001) and macroscopically unaffected oral mucosa (P < 0.001) were correlated with shorter disease-specific survival.",
journal = "International Journal of Oral and Maxillofacial Surgery",
title = "Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer",
volume = "47",
number = "8",
pages = "976-982",
doi = "10.1016/j.ijom.2018.01.020"
}

Eljabo, N., Nikolić, N., Čarkić, J., Jelovac, D. B., Lazarević, M. M., Tanić, N.,& Milašin, J.. (2018). Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer. in International Journal of Oral and Maxillofacial Surgery, 47(8), 976-982.
https://doi.org/10.1016/j.ijom.2018.01.020

Eljabo N, Nikolić N, Čarkić J, Jelovac DB, Lazarević MM, Tanić N, Milašin J. Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer. in International Journal of Oral and Maxillofacial Surgery. 2018;47(8):976-982.
doi:10.1016/j.ijom.2018.01.020 .

Eljabo, Najib, Nikolić, Nađa, Čarkić, Jelena, Jelovac, Drago B., Lazarević, Miloš M., Tanić, Nasta, Milašin, Jelena, "Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer" in International Journal of Oral and Maxillofacial Surgery, 47, no. 8 (2018):976-982,
https://doi.org/10.1016/j.ijom.2018.01.020 . .