TY  - JOUR
AU  - Preljević, Kenan
AU  - Pašić, Ivana
AU  - Vlaović, Milorad
AU  - Matić, Ivana Z.
AU  - Krivokapić, Slađana
AU  - Petrović, Nina
AU  - Stanojković, Tatjana
AU  - Živković, Vladimir
AU  - Perović, Svetlana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12935
AB  - The essential oils of Thymus vulgaris (TVEO) and Thymus serpyllum (TSEO) show different biological activities. The aim of the study was to evaluate the biological activities of TVEO and TSEO from Montenegro. The main components of TVEO were p-cymene (29.52%), thymol (22.8%) and linalool (4.73%) while the main components of TSEO were p-cymene (19.04%), geraniol (11,09%), linalool (9.16%), geranyl acetate (6.49%) and borneol (5.24%). Antioxidant activity determined via DPPH for TVEO was 4.49 and FRAP 1130.27, while for TSEO it was estimated that DPPH was 4.88 μL/mL and FRAP was 701.25 μmol FRAP/L. Both essential oils were active against all tested bacteria, with the highest level of sensitivity of E. coli with MIC of 1.5625 μL/mL. Essential oils showed strong cytotoxic effects on human cancer cell lines, with IC50 values ranging from 0.20 to 0.24 μL/mL for TVEO and from 0.32 to 0.49 μL/mL for TSEO. TVEO caused apoptosis in cervical adenocarcinoma HeLa cells through activation of caspase-3 and caspase-8, while TSEO caused apoptosis through caspase-3. EOs decreased levels of oxidative stress in normal MRC-5 cells. HeLa cells treated with TVEO had reduced MMP2 expression levels, while cells treated with TSEO had lowered MMP2 and MMP9 levels. The treatment of HeLa cells with TVEO increased the levels of miR-16 and miR-34a, indicating potential tumor-suppressive properties. Our findings suggest that Thymus essential oils may be considered as good candidates for further investigation as cancer-chemopreventive and cancer-therapeutic agents.
T2  - Fitoterapia
T1  - Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro
VL  - 174
SP  - 105871
DO  - 10.1016/j.fitote.2024.105871
ER  - 

@article{
author = "Preljević, Kenan and Pašić, Ivana and Vlaović, Milorad and Matić, Ivana Z. and Krivokapić, Slađana and Petrović, Nina and Stanojković, Tatjana and Živković, Vladimir and Perović, Svetlana",
year = "2024",
abstract = "The essential oils of Thymus vulgaris (TVEO) and Thymus serpyllum (TSEO) show different biological activities. The aim of the study was to evaluate the biological activities of TVEO and TSEO from Montenegro. The main components of TVEO were p-cymene (29.52%), thymol (22.8%) and linalool (4.73%) while the main components of TSEO were p-cymene (19.04%), geraniol (11,09%), linalool (9.16%), geranyl acetate (6.49%) and borneol (5.24%). Antioxidant activity determined via DPPH for TVEO was 4.49 and FRAP 1130.27, while for TSEO it was estimated that DPPH was 4.88 μL/mL and FRAP was 701.25 μmol FRAP/L. Both essential oils were active against all tested bacteria, with the highest level of sensitivity of E. coli with MIC of 1.5625 μL/mL. Essential oils showed strong cytotoxic effects on human cancer cell lines, with IC50 values ranging from 0.20 to 0.24 μL/mL for TVEO and from 0.32 to 0.49 μL/mL for TSEO. TVEO caused apoptosis in cervical adenocarcinoma HeLa cells through activation of caspase-3 and caspase-8, while TSEO caused apoptosis through caspase-3. EOs decreased levels of oxidative stress in normal MRC-5 cells. HeLa cells treated with TVEO had reduced MMP2 expression levels, while cells treated with TSEO had lowered MMP2 and MMP9 levels. The treatment of HeLa cells with TVEO increased the levels of miR-16 and miR-34a, indicating potential tumor-suppressive properties. Our findings suggest that Thymus essential oils may be considered as good candidates for further investigation as cancer-chemopreventive and cancer-therapeutic agents.",
journal = "Fitoterapia",
title = "Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro",
volume = "174",
pages = "105871",
doi = "10.1016/j.fitote.2024.105871"
}

Preljević, K., Pašić, I., Vlaović, M., Matić, I. Z., Krivokapić, S., Petrović, N., Stanojković, T., Živković, V.,& Perović, S.. (2024). Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro. in Fitoterapia, 174, 105871.
https://doi.org/10.1016/j.fitote.2024.105871

Preljević K, Pašić I, Vlaović M, Matić IZ, Krivokapić S, Petrović N, Stanojković T, Živković V, Perović S. Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro. in Fitoterapia. 2024;174:105871.
doi:10.1016/j.fitote.2024.105871 .

Preljević, Kenan, Pašić, Ivana, Vlaović, Milorad, Matić, Ivana Z., Krivokapić, Slađana, Petrović, Nina, Stanojković, Tatjana, Živković, Vladimir, Perović, Svetlana, "Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro" in Fitoterapia, 174 (2024):105871,
https://doi.org/10.1016/j.fitote.2024.105871 . .

TY  - CONF
AU  - Pašić, Ivana
AU  - Preljević, Kenan
AU  - Matić, Ivana
AU  - Petrović, Nina
AU  - Stanojković, Tatjana
AU  - Perović, Svetlana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12566
AB  - Background: It has been demonstrated that aromaƟ c plant essenƟ al oils (EO) contain phytochemicals with anƟ - infl ammatory, anƟ oxidant, and anƟ cancer acƟ viƟ es. The goal of this study was to examine the cytotoxicity and the anƟ cancer acƟ on mechanisms of an EO extracted from Satureja montana plant species, originaƟ ng from Montenegro. Material and methods: The cytotoxic acƟ vity was assessed against human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, lung carcinoma A549, as well as against normal human lung fi broblasts MRC-5 by MTT assay. Using fl ow cytometry, it was examined how HeLa cells were distributed throughout the cell cycle aŌ er treatment with EO and whether caspase-3, caspase-8, and caspase-9 were acƟ vated. RT-qPCR was used to assess the expression levels of genes and miRNA in HeLa cells. Results: Satureja montana EO exerted strong cytotoxicity against human cancer cell lines, with IC50 values ranging from 0,12 to 0,18 µL/mL, The strongest cytotoxic acƟ vity of EO was observed against lung carcinoma A549 cells with an IC50 value of 0.12 µL/mL and LS 174T colorectal adenocarcinoma cells with IC50 value of 0.13 µL/mL. The lowest cytotoxicity was determined against normal lung fi broblasts MRC-5 (IC50 value of 0.19 µL/mL). AŌ er 24 hours of treatment with Satureja montana EO, a striking increase in the proporƟ on of HeLa cells in the subG1 phase was seen in comparison with control cells. Pretreatment of HeLa cells with caspase inhibitors showed that Satureja montana EO acƟ vated all three invesƟ gated caspases to trigger apoptosis. In comparison to control cells, Satureja montana EO treatment of HeLa cells decreased MMP2 gene expression levels, and elevated MMP9 and VEGFA gene expression levels. Satureja montana EO treatment increased the expression levels of the tumor supressive miR-16 and miR-34ain HeLa cells, and increased levels of miR21, with oncogenic role in cervical cancer. Conclusion: Satureja montana EO showed strong cytotoxic eff ects on tested cancer cells. EO exerted prominent apoptoƟ c acƟ vity in HeLa cells via intrinsic and extrinsic pathways. Decrease in the expression levels of MMP2 gene, involved in invasion and metastasis, and cancer suppressive miRNAs, further support its potenƟ al to be uƟ lized as a therapeuƟ c agent in cancer treatment.
PB  - Belgrade : Serbian Association for Cancer Research
C3  - Oncology Insights
T1  - Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro
IS  - 1
SP  - 67
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12566
ER  - 

@conference{
author = "Pašić, Ivana and Preljević, Kenan and Matić, Ivana and Petrović, Nina and Stanojković, Tatjana and Perović, Svetlana",
year = "2023",
abstract = "Background: It has been demonstrated that aromaƟ c plant essenƟ al oils (EO) contain phytochemicals with anƟ - infl ammatory, anƟ oxidant, and anƟ cancer acƟ viƟ es. The goal of this study was to examine the cytotoxicity and the anƟ cancer acƟ on mechanisms of an EO extracted from Satureja montana plant species, originaƟ ng from Montenegro. Material and methods: The cytotoxic acƟ vity was assessed against human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, lung carcinoma A549, as well as against normal human lung fi broblasts MRC-5 by MTT assay. Using fl ow cytometry, it was examined how HeLa cells were distributed throughout the cell cycle aŌ er treatment with EO and whether caspase-3, caspase-8, and caspase-9 were acƟ vated. RT-qPCR was used to assess the expression levels of genes and miRNA in HeLa cells. Results: Satureja montana EO exerted strong cytotoxicity against human cancer cell lines, with IC50 values ranging from 0,12 to 0,18 µL/mL, The strongest cytotoxic acƟ vity of EO was observed against lung carcinoma A549 cells with an IC50 value of 0.12 µL/mL and LS 174T colorectal adenocarcinoma cells with IC50 value of 0.13 µL/mL. The lowest cytotoxicity was determined against normal lung fi broblasts MRC-5 (IC50 value of 0.19 µL/mL). AŌ er 24 hours of treatment with Satureja montana EO, a striking increase in the proporƟ on of HeLa cells in the subG1 phase was seen in comparison with control cells. Pretreatment of HeLa cells with caspase inhibitors showed that Satureja montana EO acƟ vated all three invesƟ gated caspases to trigger apoptosis. In comparison to control cells, Satureja montana EO treatment of HeLa cells decreased MMP2 gene expression levels, and elevated MMP9 and VEGFA gene expression levels. Satureja montana EO treatment increased the expression levels of the tumor supressive miR-16 and miR-34ain HeLa cells, and increased levels of miR21, with oncogenic role in cervical cancer. Conclusion: Satureja montana EO showed strong cytotoxic eff ects on tested cancer cells. EO exerted prominent apoptoƟ c acƟ vity in HeLa cells via intrinsic and extrinsic pathways. Decrease in the expression levels of MMP2 gene, involved in invasion and metastasis, and cancer suppressive miRNAs, further support its potenƟ al to be uƟ lized as a therapeuƟ c agent in cancer treatment.",
publisher = "Belgrade : Serbian Association for Cancer Research",
journal = "Oncology Insights",
title = "Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro",
number = "1",
pages = "67-68",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12566"
}

Pašić, I., Preljević, K., Matić, I., Petrović, N., Stanojković, T.,& Perović, S.. (2023). Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro. in Oncology Insights
Belgrade : Serbian Association for Cancer Research.(1), 67-68.
https://hdl.handle.net/21.15107/rcub_vinar_12566

Pašić I, Preljević K, Matić I, Petrović N, Stanojković T, Perović S. Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro. in Oncology Insights. 2023;(1):67-68.
https://hdl.handle.net/21.15107/rcub_vinar_12566 .

Pašić, Ivana, Preljević, Kenan, Matić, Ivana, Petrović, Nina, Stanojković, Tatjana, Perović, Svetlana, "Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro" in Oncology Insights, no. 1 (2023):67-68,
https://hdl.handle.net/21.15107/rcub_vinar_12566 .