TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Bajić, Vladan P.
AU  - Đorđević, Stefana
AU  - Hristov, Aleksandar
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9002
AB  - Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078-2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078-10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers.
AB  - Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) - skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.
T2  - Medicinski časopis
T1  - Evaluation of antioxidant potential of Cordyceps sinensis in vitro
T1  - Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro
VL  - 53
IS  - 4
SP  - 129
EP  - 134
DO  - 10.5937/mckg53-24450
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica Z. and Bajić, Vladan P. and Đorđević, Stefana and Hristov, Aleksandar and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078-2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078-10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers., Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) - skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.",
journal = "Medicinski časopis",
title = "Evaluation of antioxidant potential of Cordyceps sinensis in vitro, Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro",
volume = "53",
number = "4",
pages = "129-134",
doi = "10.5937/mckg53-24450"
}

Živković, L., Borozan, S. Z., Bajić, V. P., Đorđević, S., Hristov, A.,& Spremo-Potparević, B.. (2019). Evaluation of antioxidant potential of Cordyceps sinensis in vitro. in Medicinski časopis, 53(4), 129-134.
https://doi.org/10.5937/mckg53-24450

Živković L, Borozan SZ, Bajić VP, Đorđević S, Hristov A, Spremo-Potparević B. Evaluation of antioxidant potential of Cordyceps sinensis in vitro. in Medicinski časopis. 2019;53(4):129-134.
doi:10.5937/mckg53-24450 .

Živković, Lada, Borozan, Sunčica Z., Bajić, Vladan P., Đorđević, Stefana, Hristov, Aleksandar, Spremo-Potparević, Biljana, "Evaluation of antioxidant potential of Cordyceps sinensis in vitro" in Medicinski časopis, 53, no. 4 (2019):129-134,
https://doi.org/10.5937/mckg53-24450 . .

TY  - JOUR
AU  - Stojanović, Marija
AU  - Todorović, Dajana D.
AU  - Šćepanović, Ljiljana
AU  - Mitrović, Dušan M.
AU  - Borozan, Sunčica Z.
AU  - Dragutinović, Vesna V.
AU  - Labudović-Borović, Milica
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-018-3311-2
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8060
AB  - The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.
T2  - Molecular and Cellular Biochemistry
T1  - Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue
VL  - 448
IS  - 1-2
SP  - 43
EP  - 50
DO  - 10.1007/s11010-018-3311-2
ER  - 

@article{
author = "Stojanović, Marija and Todorović, Dajana D. and Šćepanović, Ljiljana and Mitrović, Dušan M. and Borozan, Sunčica Z. and Dragutinović, Vesna V. and Labudović-Borović, Milica and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.",
journal = "Molecular and Cellular Biochemistry",
title = "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue",
volume = "448",
number = "1-2",
pages = "43-50",
doi = "10.1007/s11010-018-3311-2"
}

Stojanović, M., Todorović, D. D., Šćepanović, L., Mitrović, D. M., Borozan, S. Z., Dragutinović, V. V., Labudović-Borović, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry, 448(1-2), 43-50.
https://doi.org/10.1007/s11010-018-3311-2

Stojanović M, Todorović DD, Šćepanović L, Mitrović DM, Borozan SZ, Dragutinović VV, Labudović-Borović M, Krstić DZ, Čolović MB, Đurić DM. Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry. 2018;448(1-2):43-50.
doi:10.1007/s11010-018-3311-2 .

Stojanović, Marija, Todorović, Dajana D., Šćepanović, Ljiljana, Mitrović, Dušan M., Borozan, Sunčica Z., Dragutinović, Vesna V., Labudović-Borović, Milica, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue" in Molecular and Cellular Biochemistry, 448, no. 1-2 (2018):43-50,
https://doi.org/10.1007/s11010-018-3311-2 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Čabarkapa, Andrea
AU  - Topalović, Dijana
AU  - Ciptasari, Ummi
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1458
AB  - The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells
DO  - 10.1155/2017/8759764
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica Z. and Čabarkapa, Andrea and Topalović, Dijana and Ciptasari, Ummi and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2017",
abstract = "The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells",
doi = "10.1155/2017/8759764"
}

Živković, L., Borozan, S. Z., Čabarkapa, A., Topalović, D., Ciptasari, U., Bajić, V. P.,& Spremo-Potparević, B.. (2017). Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity.
https://doi.org/10.1155/2017/8759764

Živković L, Borozan SZ, Čabarkapa A, Topalović D, Ciptasari U, Bajić VP, Spremo-Potparević B. Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity. 2017;.
doi:10.1155/2017/8759764 .

Živković, Lada, Borozan, Sunčica Z., Čabarkapa, Andrea, Topalović, Dijana, Ciptasari, Ummi, Bajić, Vladan P., Spremo-Potparević, Biljana, "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells" in Oxidative Medicine and Cellular Longevity (2017),
https://doi.org/10.1155/2017/8759764 . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Zlatković-Svenda, Mirjana
AU  - Dekanski, Dragana
AU  - Jancic, Ivan
AU  - Radak-Perović, Marija
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1282
AB  - The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright (c) 2016 John Wiley and Sons, Ltd.
T2  - Phytotherapy Research
T1  - Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study
VL  - 30
IS  - 10
SP  - 1615
EP  - 1623
DO  - 10.1002/ptr.5662
ER  - 

@article{
author = "Čabarkapa, Andrea and Živković, Lada and Borozan, Sunčica Z. and Zlatković-Svenda, Mirjana and Dekanski, Dragana and Jancic, Ivan and Radak-Perović, Marija and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2016",
abstract = "The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright (c) 2016 John Wiley and Sons, Ltd.",
journal = "Phytotherapy Research",
title = "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study",
volume = "30",
number = "10",
pages = "1615-1623",
doi = "10.1002/ptr.5662"
}

Čabarkapa, A., Živković, L., Borozan, S. Z., Zlatković-Svenda, M., Dekanski, D., Jancic, I., Radak-Perović, M., Bajić, V. P.,& Spremo-Potparević, B.. (2016). Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research, 30(10), 1615-1623.
https://doi.org/10.1002/ptr.5662

Čabarkapa A, Živković L, Borozan SZ, Zlatković-Svenda M, Dekanski D, Jancic I, Radak-Perović M, Bajić VP, Spremo-Potparević B. Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research. 2016;30(10):1615-1623.
doi:10.1002/ptr.5662 .

Čabarkapa, Andrea, Živković, Lada, Borozan, Sunčica Z., Zlatković-Svenda, Mirjana, Dekanski, Dragana, Jancic, Ivan, Radak-Perović, Marija, Bajić, Vladan P., Spremo-Potparević, Biljana, "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study" in Phytotherapy Research, 30, no. 10 (2016):1615-1623,
https://doi.org/10.1002/ptr.5662 . .

TY  - JOUR
AU  - Čabarkapa-Pirković, Andrea 
AU  - Borozan, Sunčica Z.
AU  - Živković, Lada
AU  - Stojanovic, Srdan D.
AU  - Milanović-Čabarkapa, Mirjana
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/873
AB  - Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study
VL  - 242
SP  - 171
EP  - 178
DO  - 10.1016/j.cbi.2015.10.002
ER  - 

@article{
author = "Čabarkapa-Pirković, Andrea  and Borozan, Sunčica Z. and Živković, Lada and Stojanovic, Srdan D. and Milanović-Čabarkapa, Mirjana and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2015",
abstract = "Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study",
volume = "242",
pages = "171-178",
doi = "10.1016/j.cbi.2015.10.002"
}

Čabarkapa-Pirković, A., Borozan, S. Z., Živković, L., Stojanovic, S. D., Milanović-Čabarkapa, M., Bajić, V. P.,& Spremo-Potparević, B.. (2015). CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions
Elsevier., 242, 171-178.
https://doi.org/10.1016/j.cbi.2015.10.002

Čabarkapa-Pirković A, Borozan SZ, Živković L, Stojanovic SD, Milanović-Čabarkapa M, Bajić VP, Spremo-Potparević B. CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions. 2015;242:171-178.
doi:10.1016/j.cbi.2015.10.002 .

Čabarkapa-Pirković, Andrea , Borozan, Sunčica Z., Živković, Lada, Stojanovic, Srdan D., Milanović-Čabarkapa, Mirjana, Bajić, Vladan P., Spremo-Potparević, Biljana, "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study" in Chemico-Biological Interactions, 242 (2015):171-178,
https://doi.org/10.1016/j.cbi.2015.10.002 . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Borozan, Sunčica Z.
AU  - Živković, Lada
AU  - Milanovic-Cabarkapa, Mirjana
AU  - Stojanovic, Srdan
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/703
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
T2  - Toxicological and Environmental Chemistry
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 

@article{
author = "Čabarkapa, Andrea and Borozan, Sunčica Z. and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Stojanovic, Srdan and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
journal = "Toxicological and Environmental Chemistry",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}

Čabarkapa, A., Borozan, S. Z., Živković, L., Milanovic-Cabarkapa, M., Stojanovic, S., Bajić, V. P.,& Spremo-Potparević, B.. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicological and Environmental Chemistry, 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973

Čabarkapa A, Borozan SZ, Živković L, Milanovic-Cabarkapa M, Stojanovic S, Bajić VP, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicological and Environmental Chemistry. 2015;97(6):799-813.
doi:10.1080/02772248.2015.1060973 .

Čabarkapa, Andrea, Borozan, Sunčica Z., Živković, Lada, Milanovic-Cabarkapa, Mirjana, Stojanovic, Srdan, Bajić, Vladan P., Spremo-Potparević, Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" in Toxicological and Environmental Chemistry, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 . .

TY  - JOUR
AU  - Božić, Tatjana P.
AU  - Stevanović, Jelka Ž.
AU  - Kovačević-Filipović, Milica M.
AU  - Borozan, Sunčica Z.
AU  - Popović, Dragana Lj.
AU  - Todorović, Dragana
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8767
AB  - The paper presents preliminary results of the study on environmental and health effects due to the use of depleted uranium (DU) ammunition during NATO bombing of Serbia and Montenegro in 1999. Samples of animal blood (sheep, cows) were randomly collected in the region of Bujanovac (Novo Selo, Borovac) in 2003. Biochemical and hematological parameters of peripheral blood (oxidase activity of ceruloplasmin, malondialdehide concentration of red blood cell, activity of erythrocytic superoxide dismutase, concentration of hemoglobin, red blood cells count, etc.) indicating possible effects of oxidative stress due to the impact of DU on the environment were analyzed. The results have been evaluated in respect to the effect of the environmental DU.
T2  - Central European Journal of Occupational and Environmetal Medicine
T1  - Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas
VL  - 9
IS  - 4
SP  - 267
EP  - 272
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8767
ER  - 

@article{
author = "Božić, Tatjana P. and Stevanović, Jelka Ž. and Kovačević-Filipović, Milica M. and Borozan, Sunčica Z. and Popović, Dragana Lj. and Todorović, Dragana",
year = "2003",
abstract = "The paper presents preliminary results of the study on environmental and health effects due to the use of depleted uranium (DU) ammunition during NATO bombing of Serbia and Montenegro in 1999. Samples of animal blood (sheep, cows) were randomly collected in the region of Bujanovac (Novo Selo, Borovac) in 2003. Biochemical and hematological parameters of peripheral blood (oxidase activity of ceruloplasmin, malondialdehide concentration of red blood cell, activity of erythrocytic superoxide dismutase, concentration of hemoglobin, red blood cells count, etc.) indicating possible effects of oxidative stress due to the impact of DU on the environment were analyzed. The results have been evaluated in respect to the effect of the environmental DU.",
journal = "Central European Journal of Occupational and Environmetal Medicine",
title = "Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas",
volume = "9",
number = "4",
pages = "267-272",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8767"
}

Božić, T. P., Stevanović, J. Ž., Kovačević-Filipović, M. M., Borozan, S. Z., Popović, D. Lj.,& Todorović, D.. (2003). Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas. in Central European Journal of Occupational and Environmetal Medicine, 9(4), 267-272.
https://hdl.handle.net/21.15107/rcub_vinar_8767

Božić TP, Stevanović JŽ, Kovačević-Filipović MM, Borozan SZ, Popović DL, Todorović D. Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas. in Central European Journal of Occupational and Environmetal Medicine. 2003;9(4):267-272.
https://hdl.handle.net/21.15107/rcub_vinar_8767 .

Božić, Tatjana P., Stevanović, Jelka Ž., Kovačević-Filipović, Milica M., Borozan, Sunčica Z., Popović, Dragana Lj., Todorović, Dragana, "Possible Effects of Depleted Uranium (DU): I. Changes in Cellular and Biochemical Values in Peripheral Blood of Ruminants in Exposed Areas" in Central European Journal of Occupational and Environmetal Medicine, 9, no. 4 (2003):267-272,
https://hdl.handle.net/21.15107/rcub_vinar_8767 .