TY  - JOUR
AU  - Mitrović, Aleksandra Lj.
AU  - Simonović-Radosavljević, Jasna
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Kovačević, Jovana
AU  - Prodanović, Olivera
AU  - Todorović, Bratislav
AU  - Matović, Branko
AU  - Stanković, Mira
AU  - Maksimović, Vuk
AU  - Mutavdžić, Dragosav
AU  - Skočić, Miloš
AU  - Pešić, Mirjana
AU  - Prokić, Ljiljana
AU  - Radotić, Ksenija
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9129
AB  - The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day- UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C[dbnd]C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV–B), and ionic POD and covalent PO (UV–C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling. © 2021 Elsevier Masson SAS
T2  - Plant Physiology and Biochemistry
T1  - Cell wall response to UV radiation in needles of Picea omorika
VL  - 161
SP  - 176
EP  - 190
DO  - 10.1016/j.plaphy.2021.02.007
ER  - 

@article{
author = "Mitrović, Aleksandra Lj. and Simonović-Radosavljević, Jasna and Prokopijević, Miloš and Spasojević, Dragica and Kovačević, Jovana and Prodanović, Olivera and Todorović, Bratislav and Matović, Branko and Stanković, Mira and Maksimović, Vuk and Mutavdžić, Dragosav and Skočić, Miloš and Pešić, Mirjana and Prokić, Ljiljana and Radotić, Ksenija",
year = "2021",
abstract = "The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day- UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C[dbnd]C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV–B), and ionic POD and covalent PO (UV–C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling. © 2021 Elsevier Masson SAS",
journal = "Plant Physiology and Biochemistry",
title = "Cell wall response to UV radiation in needles of Picea omorika",
volume = "161",
pages = "176-190",
doi = "10.1016/j.plaphy.2021.02.007"
}

Mitrović, A. Lj., Simonović-Radosavljević, J., Prokopijević, M., Spasojević, D., Kovačević, J., Prodanović, O., Todorović, B., Matović, B., Stanković, M., Maksimović, V., Mutavdžić, D., Skočić, M., Pešić, M., Prokić, L.,& Radotić, K.. (2021). Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry, 161, 176-190.
https://doi.org/10.1016/j.plaphy.2021.02.007

Mitrović AL, Simonović-Radosavljević J, Prokopijević M, Spasojević D, Kovačević J, Prodanović O, Todorović B, Matović B, Stanković M, Maksimović V, Mutavdžić D, Skočić M, Pešić M, Prokić L, Radotić K. Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry. 2021;161:176-190.
doi:10.1016/j.plaphy.2021.02.007 .

Mitrović, Aleksandra Lj., Simonović-Radosavljević, Jasna, Prokopijević, Miloš, Spasojević, Dragica, Kovačević, Jovana, Prodanović, Olivera, Todorović, Bratislav, Matović, Branko, Stanković, Mira, Maksimović, Vuk, Mutavdžić, Dragosav, Skočić, Miloš, Pešić, Mirjana, Prokić, Ljiljana, Radotić, Ksenija, "Cell wall response to UV radiation in needles of Picea omorika" in Plant Physiology and Biochemistry, 161 (2021):176-190,
https://doi.org/10.1016/j.plaphy.2021.02.007 . .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Spasojević, Dragica
AU  - Orlovska, Irina V.
AU  - Kozyrovska, Natalia O.
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Dmitrović, Svetlana
AU  - Matović, Branko
AU  - Tasić, Nikola B.
AU  - Maksimović, Vuk M.
AU  - Sosnin, Mikhail
AU  - Radotić, Ksenija
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7769
AB  - Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.
T2  - International Journal of Biological Macromolecules
T1  - Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing
VL  - 118
SP  - 494
EP  - 503
DO  - 10.1016/j.ijbiomac.2018.06.067
ER  - 

@article{
author = "Zmejkoski, Danica and Spasojević, Dragica and Orlovska, Irina V. and Kozyrovska, Natalia O. and Soković, Marina and Glamočlija, Jasmina and Dmitrović, Svetlana and Matović, Branko and Tasić, Nikola B. and Maksimović, Vuk M. and Sosnin, Mikhail and Radotić, Ksenija",
year = "2018",
abstract = "Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.",
journal = "International Journal of Biological Macromolecules",
title = "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing",
volume = "118",
pages = "494-503",
doi = "10.1016/j.ijbiomac.2018.06.067"
}

Zmejkoski, D., Spasojević, D., Orlovska, I. V., Kozyrovska, N. O., Soković, M., Glamočlija, J., Dmitrović, S., Matović, B., Tasić, N. B., Maksimović, V. M., Sosnin, M.,& Radotić, K.. (2018). Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules, 118, 494-503.
https://doi.org/10.1016/j.ijbiomac.2018.06.067

Zmejkoski D, Spasojević D, Orlovska IV, Kozyrovska NO, Soković M, Glamočlija J, Dmitrović S, Matović B, Tasić NB, Maksimović VM, Sosnin M, Radotić K. Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules. 2018;118:494-503.
doi:10.1016/j.ijbiomac.2018.06.067 .

Zmejkoski, Danica, Spasojević, Dragica, Orlovska, Irina V., Kozyrovska, Natalia O., Soković, Marina, Glamočlija, Jasmina, Dmitrović, Svetlana, Matović, Branko, Tasić, Nikola B., Maksimović, Vuk M., Sosnin, Mikhail, Radotić, Ksenija, "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing" in International Journal of Biological Macromolecules, 118 (2018):494-503,
https://doi.org/10.1016/j.ijbiomac.2018.06.067 . .