TY  - JOUR
AU  - Todorović, Lidija
AU  - Stamenković, Gorana
AU  - Vučetić-Tadić, Biljana
AU  - Umezawa, Kazuo
AU  - Božović, Ana M.
AU  - Yamashita, Shunichi
AU  - Stanojević, Boban
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9413
AB  - The use of targeted inhibitors has shown promise as an effective approach in cancer therapy. However, targeted therapies based only on one drug, such as 17-allylamino-17-demethoxygeldanamycin (17-AAG), have limited success, partly because cancer cells engage alternate pathways for survival and proliferation. In the present study, we evaluated whether dehydroxymethylepoxyquinomicin (DHMEQ), a nuclear factor ?B (NF-?B) inhibitor, can enhance the antitumor activities of 17-AAG, a 90 kDa heat shock protein (Hsp90) inhibitor, in the anaplastic thyroid cancer cell line KTC2. We examined the effect of combined drug treatment vs single drug treatment on cell survival. Isobologram analysis was performed to distinguish the additive vs synergistic effects of the drug combination. Western blotting was performed to investigate apoptosis markers: caspase 3, poly(ADP-ribose) polymerase-one (PARP-1), B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis (XIAP) and cellular inhibitor of apoptosis 2 (cIAP-2). Compared to monotherapy, the combined treatment enhanced growth-inhibitory effects in a synergistic manner and strongly potentiated apoptosis. These results demonstrate the first in vitro evidence that a combination of Hsp90 and NF-?B inhibitors is a more effective modality for inhibiting cell proliferation and survival in anaplastic thyroid carcinoma cells than either agent alone, warranting further investigations.
T2  - Archives of Biological Sciences
T1  - Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2
VL  - 73
IS  - 1
SP  - 31
EP  - 38
DO  - 10.2298/ABS201010055T
ER  - 

@article{
author = "Todorović, Lidija and Stamenković, Gorana and Vučetić-Tadić, Biljana and Umezawa, Kazuo and Božović, Ana M. and Yamashita, Shunichi and Stanojević, Boban",
year = "2021",
abstract = "The use of targeted inhibitors has shown promise as an effective approach in cancer therapy. However, targeted therapies based only on one drug, such as 17-allylamino-17-demethoxygeldanamycin (17-AAG), have limited success, partly because cancer cells engage alternate pathways for survival and proliferation. In the present study, we evaluated whether dehydroxymethylepoxyquinomicin (DHMEQ), a nuclear factor ?B (NF-?B) inhibitor, can enhance the antitumor activities of 17-AAG, a 90 kDa heat shock protein (Hsp90) inhibitor, in the anaplastic thyroid cancer cell line KTC2. We examined the effect of combined drug treatment vs single drug treatment on cell survival. Isobologram analysis was performed to distinguish the additive vs synergistic effects of the drug combination. Western blotting was performed to investigate apoptosis markers: caspase 3, poly(ADP-ribose) polymerase-one (PARP-1), B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis (XIAP) and cellular inhibitor of apoptosis 2 (cIAP-2). Compared to monotherapy, the combined treatment enhanced growth-inhibitory effects in a synergistic manner and strongly potentiated apoptosis. These results demonstrate the first in vitro evidence that a combination of Hsp90 and NF-?B inhibitors is a more effective modality for inhibiting cell proliferation and survival in anaplastic thyroid carcinoma cells than either agent alone, warranting further investigations.",
journal = "Archives of Biological Sciences",
title = "Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2",
volume = "73",
number = "1",
pages = "31-38",
doi = "10.2298/ABS201010055T"
}

Todorović, L., Stamenković, G., Vučetić-Tadić, B., Umezawa, K., Božović, A. M., Yamashita, S.,& Stanojević, B.. (2021). Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2. in Archives of Biological Sciences, 73(1), 31-38.
https://doi.org/10.2298/ABS201010055T

Todorović L, Stamenković G, Vučetić-Tadić B, Umezawa K, Božović AM, Yamashita S, Stanojević B. Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2. in Archives of Biological Sciences. 2021;73(1):31-38.
doi:10.2298/ABS201010055T .

Todorović, Lidija, Stamenković, Gorana, Vučetić-Tadić, Biljana, Umezawa, Kazuo, Božović, Ana M., Yamashita, Shunichi, Stanojević, Boban, "Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2" in Archives of Biological Sciences, 73, no. 1 (2021):31-38,
https://doi.org/10.2298/ABS201010055T . .

TY  - JOUR
AU  - Stanojević, Boban
AU  - Osiowy, Carla
AU  - Schaefer, Stephan
AU  - Bojovic, Ksenija
AU  - Blagojevic, Jelena
AU  - Nesic, Milica
AU  - Yamashita, Shunichi
AU  - Stamenković, Gorana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4450
AB  - Hepatitis B virus (HBV) is classified into 8 genotypes with distinct geographical distribution. Genotype D (HBV/D) has the widest distribution area and is comprised of 7 subgenotypes. Subgenotypes D1, D2 and D3 appear worldwide, while D4-D7 have a more restricted distribution. Within the Mediterranean area, HBV/D and subgenotype D3 are the most prevalent. The purpose of this study was to characterize the full genome of Serbian HBV/D3 isolates by comparison and phylogenetic analysis with HBV/D3 sequences (66 samples) found in GeneBank/DDBJ databases from different parts of the world. Isolates were obtained from three patients diagnosed with chronic hepatitis B (HBsAg +). All three isolates have two very rare nucleotide substitutions, A929T and T150A, which indicate the same ancestor. Phylogenetic analysis of HBV/D3 genome sequences throughout the world follows an ethno-geographical origin of isolates with rare exceptions, which could be explained by human travelling and migration. The geographically close but ethnically different Serbian and Italian isolates clustered in the same subnode, and on a common branch with strains from Northern Canada. To test the apparently close HBV phylogenetic relationship between completely separated patients from Serbia and Northern Canada we analyzed in depth a 440 bp region of the HBsAg from Canadian (n = 73) and Serbian (n = 70) isolates. The constructed parsimony tree revealed that strains from Serbia and Northern Canada fell along the same branch which indicates independent evolution within regions of each country, Considering that HBsAg sequence has limited variability for phylogenetic analyses, our hypothesis needs further confirmation with more HBV complete genome sequences. (C) 2011 Elsevier B.V. All rights reserved,
T2  - Infection Genetics and Evolution
T1  - Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia
VL  - 11
IS  - 6
SP  - 1475
EP  - 1480
DO  - 10.1016/j.meegid.2011.05.004
ER  - 

@article{
author = "Stanojević, Boban and Osiowy, Carla and Schaefer, Stephan and Bojovic, Ksenija and Blagojevic, Jelena and Nesic, Milica and Yamashita, Shunichi and Stamenković, Gorana",
year = "2011",
abstract = "Hepatitis B virus (HBV) is classified into 8 genotypes with distinct geographical distribution. Genotype D (HBV/D) has the widest distribution area and is comprised of 7 subgenotypes. Subgenotypes D1, D2 and D3 appear worldwide, while D4-D7 have a more restricted distribution. Within the Mediterranean area, HBV/D and subgenotype D3 are the most prevalent. The purpose of this study was to characterize the full genome of Serbian HBV/D3 isolates by comparison and phylogenetic analysis with HBV/D3 sequences (66 samples) found in GeneBank/DDBJ databases from different parts of the world. Isolates were obtained from three patients diagnosed with chronic hepatitis B (HBsAg +). All three isolates have two very rare nucleotide substitutions, A929T and T150A, which indicate the same ancestor. Phylogenetic analysis of HBV/D3 genome sequences throughout the world follows an ethno-geographical origin of isolates with rare exceptions, which could be explained by human travelling and migration. The geographically close but ethnically different Serbian and Italian isolates clustered in the same subnode, and on a common branch with strains from Northern Canada. To test the apparently close HBV phylogenetic relationship between completely separated patients from Serbia and Northern Canada we analyzed in depth a 440 bp region of the HBsAg from Canadian (n = 73) and Serbian (n = 70) isolates. The constructed parsimony tree revealed that strains from Serbia and Northern Canada fell along the same branch which indicates independent evolution within regions of each country, Considering that HBsAg sequence has limited variability for phylogenetic analyses, our hypothesis needs further confirmation with more HBV complete genome sequences. (C) 2011 Elsevier B.V. All rights reserved,",
journal = "Infection Genetics and Evolution",
title = "Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia",
volume = "11",
number = "6",
pages = "1475-1480",
doi = "10.1016/j.meegid.2011.05.004"
}

Stanojević, B., Osiowy, C., Schaefer, S., Bojovic, K., Blagojevic, J., Nesic, M., Yamashita, S.,& Stamenković, G.. (2011). Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia. in Infection Genetics and Evolution, 11(6), 1475-1480.
https://doi.org/10.1016/j.meegid.2011.05.004

Stanojević B, Osiowy C, Schaefer S, Bojovic K, Blagojevic J, Nesic M, Yamashita S, Stamenković G. Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia. in Infection Genetics and Evolution. 2011;11(6):1475-1480.
doi:10.1016/j.meegid.2011.05.004 .

Stanojević, Boban, Osiowy, Carla, Schaefer, Stephan, Bojovic, Ksenija, Blagojevic, Jelena, Nesic, Milica, Yamashita, Shunichi, Stamenković, Gorana, "Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia" in Infection Genetics and Evolution, 11, no. 6 (2011):1475-1480,
https://doi.org/10.1016/j.meegid.2011.05.004 . .

TY  - JOUR
AU  - Jovanović-Ćupić, Snežana P.
AU  - Simonovic-Babic, Jasmina
AU  - Blagojevic, Jelena
AU  - Božić, Milena
AU  - Ješić, Rada
AU  - Nozic, D.
AU  - Stamenković, Gorana
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3752
AB  - Different types of interferon are widely used to treat hepatitis C virus (HCV) infection. Results obtained in vitro suggest that interferon inhibits internal ribosome entry site (IRES)-mediated translation of the HCV genome. To elucidate the possible effect of the nucleotide sequence of IRES on therapy outcome, we compared HCV isolates from patients with sustained response and non-response to interferon/ribavirin combination therapy. In 56 analyzed HCV isolates, nucleotide changes appeared strictly in the stem-loop IIIb region, the stem part from 243 nt to 248 nt, and the polypyrimidine-II region. The natural sequence variability of IRES in isolates of genotype 3a was significantly higher than in isolates of genotype 1b (p LT 0.05). The average number of nucleotide changes in genotype 3a correlated with response to therapy (p LT 0.05).
T2  - Archives of Biological Sciences
T1  - Sequence Variability At the Internal Ribosome Entry Site of the Hcv Genome in Relation to Therapy Outcome
VL  - 61
IS  - 2
SP  - 205
EP  - 212
DO  - 10.2298/ABS0901205J
ER  - 

@article{
author = "Jovanović-Ćupić, Snežana P. and Simonovic-Babic, Jasmina and Blagojevic, Jelena and Božić, Milena and Ješić, Rada and Nozic, D. and Stamenković, Gorana",
year = "2009",
abstract = "Different types of interferon are widely used to treat hepatitis C virus (HCV) infection. Results obtained in vitro suggest that interferon inhibits internal ribosome entry site (IRES)-mediated translation of the HCV genome. To elucidate the possible effect of the nucleotide sequence of IRES on therapy outcome, we compared HCV isolates from patients with sustained response and non-response to interferon/ribavirin combination therapy. In 56 analyzed HCV isolates, nucleotide changes appeared strictly in the stem-loop IIIb region, the stem part from 243 nt to 248 nt, and the polypyrimidine-II region. The natural sequence variability of IRES in isolates of genotype 3a was significantly higher than in isolates of genotype 1b (p LT 0.05). The average number of nucleotide changes in genotype 3a correlated with response to therapy (p LT 0.05).",
journal = "Archives of Biological Sciences",
title = "Sequence Variability At the Internal Ribosome Entry Site of the Hcv Genome in Relation to Therapy Outcome",
volume = "61",
number = "2",
pages = "205-212",
doi = "10.2298/ABS0901205J"
}

Jovanović-Ćupić, S. P., Simonovic-Babic, J., Blagojevic, J., Božić, M., Ješić, R., Nozic, D.,& Stamenković, G.. (2009). Sequence Variability At the Internal Ribosome Entry Site of the Hcv Genome in Relation to Therapy Outcome. in Archives of Biological Sciences, 61(2), 205-212.
https://doi.org/10.2298/ABS0901205J

Jovanović-Ćupić SP, Simonovic-Babic J, Blagojevic J, Božić M, Ješić R, Nozic D, Stamenković G. Sequence Variability At the Internal Ribosome Entry Site of the Hcv Genome in Relation to Therapy Outcome. in Archives of Biological Sciences. 2009;61(2):205-212.
doi:10.2298/ABS0901205J .

Jovanović-Ćupić, Snežana P., Simonovic-Babic, Jasmina, Blagojevic, Jelena, Božić, Milena, Ješić, Rada, Nozic, D., Stamenković, Gorana, "Sequence Variability At the Internal Ribosome Entry Site of the Hcv Genome in Relation to Therapy Outcome" in Archives of Biological Sciences, 61, no. 2 (2009):205-212,
https://doi.org/10.2298/ABS0901205J . .

TY  - JOUR
AU  - Nozic, Darko
AU  - Antic, Branislav
AU  - Begovic, Vesna
AU  - Stamenković, Gorana
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3650
AB  - Vasculitis is a common extrahepatic manifestation of chronic hepatitis C virus infection and could be either associated with cryoglobulinemia or not. The mechanism of peripheral neuropathy seems to be vasculitic in both cryoglobulinemic and non-cryoglobulinemic patients. Antiviral therapy with peginterferon and ribavirin usually improves symptoms of virus-induced neuropathy. Herein, we reported on a rare case of hard mononeuritis multiplex triggered by antiviral therapy in a patient without neurological symptoms before the treatment.
T2  - Hepatitis Monthly
T1  - Severe Mononeuritis Multiplex Associated with Peginterferon-Ribavirin Therapy of Chronic Hepatitis C
VL  - 9
IS  - 1
SP  - 70
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3650
ER  - 

@article{
author = "Nozic, Darko and Antic, Branislav and Begovic, Vesna and Stamenković, Gorana",
year = "2009",
abstract = "Vasculitis is a common extrahepatic manifestation of chronic hepatitis C virus infection and could be either associated with cryoglobulinemia or not. The mechanism of peripheral neuropathy seems to be vasculitic in both cryoglobulinemic and non-cryoglobulinemic patients. Antiviral therapy with peginterferon and ribavirin usually improves symptoms of virus-induced neuropathy. Herein, we reported on a rare case of hard mononeuritis multiplex triggered by antiviral therapy in a patient without neurological symptoms before the treatment.",
journal = "Hepatitis Monthly",
title = "Severe Mononeuritis Multiplex Associated with Peginterferon-Ribavirin Therapy of Chronic Hepatitis C",
volume = "9",
number = "1",
pages = "70-72",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3650"
}

Nozic, D., Antic, B., Begovic, V.,& Stamenković, G.. (2009). Severe Mononeuritis Multiplex Associated with Peginterferon-Ribavirin Therapy of Chronic Hepatitis C. in Hepatitis Monthly, 9(1), 70-72.
https://hdl.handle.net/21.15107/rcub_vinar_3650

Nozic D, Antic B, Begovic V, Stamenković G. Severe Mononeuritis Multiplex Associated with Peginterferon-Ribavirin Therapy of Chronic Hepatitis C. in Hepatitis Monthly. 2009;9(1):70-72.
https://hdl.handle.net/21.15107/rcub_vinar_3650 .

Nozic, Darko, Antic, Branislav, Begovic, Vesna, Stamenković, Gorana, "Severe Mononeuritis Multiplex Associated with Peginterferon-Ribavirin Therapy of Chronic Hepatitis C" in Hepatitis Monthly, 9, no. 1 (2009):70-72,
https://hdl.handle.net/21.15107/rcub_vinar_3650 .

TY  - JOUR
AU  - Jovanović-Ćupić, Snežana P.
AU  - Stamenković, Gorana
AU  - Blagojevic, Jelena
AU  - Vanis, N.
AU  - Stanojević, Boban
AU  - Berberovic, Lj.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3242
AB  - The distribution of ABO blood groups and the Rhesus factor was analyzed in 279 patients who suffered from malignant tumors of the digestive system. Patients were registered retrospectively in the Gastroenterohepatology Clinic, Clinical Center, University of Sarajevo over a discontinuous period of 88 months. From the results obtained, it was concluded that: (a) men became ill from gastric cancer significantly more frequently than women; (b) the frequency of liver carcinoma was three times higher than the global frequency and the frequency neighboring ethnic groups; and (c) patients with blood group B and patients with RhD(-) exhibited a significantly higher proportion of disease.
T2  - Archives of Biological Sciences
T1  - Abo Histo-Blood Groups and Rh Systems in Relation to Malignant Tumors of the Digestive Tract in Bosnia and Herzegovina
VL  - 60
IS  - 4
SP  - 593
EP  - 599
DO  - 10.2298/ABS0804593J
ER  - 

@article{
author = "Jovanović-Ćupić, Snežana P. and Stamenković, Gorana and Blagojevic, Jelena and Vanis, N. and Stanojević, Boban and Berberovic, Lj.",
year = "2008",
abstract = "The distribution of ABO blood groups and the Rhesus factor was analyzed in 279 patients who suffered from malignant tumors of the digestive system. Patients were registered retrospectively in the Gastroenterohepatology Clinic, Clinical Center, University of Sarajevo over a discontinuous period of 88 months. From the results obtained, it was concluded that: (a) men became ill from gastric cancer significantly more frequently than women; (b) the frequency of liver carcinoma was three times higher than the global frequency and the frequency neighboring ethnic groups; and (c) patients with blood group B and patients with RhD(-) exhibited a significantly higher proportion of disease.",
journal = "Archives of Biological Sciences",
title = "Abo Histo-Blood Groups and Rh Systems in Relation to Malignant Tumors of the Digestive Tract in Bosnia and Herzegovina",
volume = "60",
number = "4",
pages = "593-599",
doi = "10.2298/ABS0804593J"
}

Jovanović-Ćupić, S. P., Stamenković, G., Blagojevic, J., Vanis, N., Stanojević, B.,& Berberovic, Lj.. (2008). Abo Histo-Blood Groups and Rh Systems in Relation to Malignant Tumors of the Digestive Tract in Bosnia and Herzegovina. in Archives of Biological Sciences, 60(4), 593-599.
https://doi.org/10.2298/ABS0804593J

Jovanović-Ćupić SP, Stamenković G, Blagojevic J, Vanis N, Stanojević B, Berberovic L. Abo Histo-Blood Groups and Rh Systems in Relation to Malignant Tumors of the Digestive Tract in Bosnia and Herzegovina. in Archives of Biological Sciences. 2008;60(4):593-599.
doi:10.2298/ABS0804593J .

Jovanović-Ćupić, Snežana P., Stamenković, Gorana, Blagojevic, Jelena, Vanis, N., Stanojević, Boban, Berberovic, Lj., "Abo Histo-Blood Groups and Rh Systems in Relation to Malignant Tumors of the Digestive Tract in Bosnia and Herzegovina" in Archives of Biological Sciences, 60, no. 4 (2008):593-599,
https://doi.org/10.2298/ABS0804593J . .

TY  - JOUR
AU  - Stamenković, Gorana
AU  - Božić, Milena
AU  - Jovanović-Ćupić, Snežana P.
AU  - Bojovic, Ksenija
AU  - Simonovic, Jasmina
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3417
T2  - Archives of Biological Sciences
T1  - Hepatitis C virus as cause of fulminant hepatitis-sequence analysis of the 5nontranslated region
VL  - 59
IS  - 3
SP  - 37P
EP  - 38P
DO  - 10.2298/ABS070337PS
ER  - 

@article{
author = "Stamenković, Gorana and Božić, Milena and Jovanović-Ćupić, Snežana P. and Bojovic, Ksenija and Simonovic, Jasmina",
year = "2007",
journal = "Archives of Biological Sciences",
title = "Hepatitis C virus as cause of fulminant hepatitis-sequence analysis of the 5nontranslated region",
volume = "59",
number = "3",
pages = "37P-38P",
doi = "10.2298/ABS070337PS"
}

Stamenković, G., Božić, M., Jovanović-Ćupić, S. P., Bojovic, K.,& Simonovic, J.. (2007). Hepatitis C virus as cause of fulminant hepatitis-sequence analysis of the 5nontranslated region. in Archives of Biological Sciences, 59(3), 37P-38P.
https://doi.org/10.2298/ABS070337PS

Stamenković G, Božić M, Jovanović-Ćupić SP, Bojovic K, Simonovic J. Hepatitis C virus as cause of fulminant hepatitis-sequence analysis of the 5nontranslated region. in Archives of Biological Sciences. 2007;59(3):37P-38P.
doi:10.2298/ABS070337PS .

Stamenković, Gorana, Božić, Milena, Jovanović-Ćupić, Snežana P., Bojovic, Ksenija, Simonovic, Jasmina, "Hepatitis C virus as cause of fulminant hepatitis-sequence analysis of the 5nontranslated region" in Archives of Biological Sciences, 59, no. 3 (2007):37P-38P,
https://doi.org/10.2298/ABS070337PS . .

TY  - JOUR
AU  - Stamenković, Gorana
AU  - Guduric, J
AU  - Veličković, Zlate S.
AU  - Skerl, Vesna
AU  - Krtolica-Žikić, Koviljka
AU  - Velikovic, E
AU  - Dimitrijević, Bogomir B.
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2483
AB  - Single-strand conformation polymorphism (SSCP) and low-stringency single specific primer (LSSP)-PCR in hepatitis C virus (HCV) genotyping were examined for informativeness and reliability. The analysis of HCV isolates included seven type 1 isolates, two type 2 isolates, and two type 3 isolates. We also analyzed five isolates that presented as mixed infections determined by type-specific PCR. Among mixed isolates, one isolate was 1a/1b and four isolates were 1b/3a. SSCP and LSSP-PCR were applied to the analysis of 5 non-coding region of HCV (-289 to -5) that contains genotype-specific sequences. Direct cycle sequencing of this region determined sequence divergences that define genotype and sequence alterations within the same genotype. Optimized conditions for the SSCP analysis clearly distinguished between genotypes 1, 2 and 3. In addition, the SSCP analysis detected sequence variants within the same genotype. However, the SSCP analysis and DNA sequencing did not confirm the presence of mixed infections. LSSP analysis, not previously employed in HCV genotyping, enabled clear distinction between genotypes 1, 2 and 3, however, this method did not differentiate between sequence variants within a genotype. Importantly, the LSSP profile demonstrated distinction between mixed infection isolates.
T2  - Clinical Chemistry and Laboratory Medicine
T1  - Analysis of 5 non-coding region in hepatitis C virus by single-strand conformation polymorphism and low-stringency single specific primer PCR
VL  - 39
IS  - 10
SP  - 948
EP  - 952
DO  - 10.1515/CCLM.2001.152
ER  - 

@article{
author = "Stamenković, Gorana and Guduric, J and Veličković, Zlate S. and Skerl, Vesna and Krtolica-Žikić, Koviljka and Velikovic, E and Dimitrijević, Bogomir B.",
year = "2001",
abstract = "Single-strand conformation polymorphism (SSCP) and low-stringency single specific primer (LSSP)-PCR in hepatitis C virus (HCV) genotyping were examined for informativeness and reliability. The analysis of HCV isolates included seven type 1 isolates, two type 2 isolates, and two type 3 isolates. We also analyzed five isolates that presented as mixed infections determined by type-specific PCR. Among mixed isolates, one isolate was 1a/1b and four isolates were 1b/3a. SSCP and LSSP-PCR were applied to the analysis of 5 non-coding region of HCV (-289 to -5) that contains genotype-specific sequences. Direct cycle sequencing of this region determined sequence divergences that define genotype and sequence alterations within the same genotype. Optimized conditions for the SSCP analysis clearly distinguished between genotypes 1, 2 and 3. In addition, the SSCP analysis detected sequence variants within the same genotype. However, the SSCP analysis and DNA sequencing did not confirm the presence of mixed infections. LSSP analysis, not previously employed in HCV genotyping, enabled clear distinction between genotypes 1, 2 and 3, however, this method did not differentiate between sequence variants within a genotype. Importantly, the LSSP profile demonstrated distinction between mixed infection isolates.",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Analysis of 5 non-coding region in hepatitis C virus by single-strand conformation polymorphism and low-stringency single specific primer PCR",
volume = "39",
number = "10",
pages = "948-952",
doi = "10.1515/CCLM.2001.152"
}

Stamenković, G., Guduric, J., Veličković, Z. S., Skerl, V., Krtolica-Žikić, K., Velikovic, E.,& Dimitrijević, B. B.. (2001). Analysis of 5 non-coding region in hepatitis C virus by single-strand conformation polymorphism and low-stringency single specific primer PCR. in Clinical Chemistry and Laboratory Medicine, 39(10), 948-952.
https://doi.org/10.1515/CCLM.2001.152

Stamenković G, Guduric J, Veličković ZS, Skerl V, Krtolica-Žikić K, Velikovic E, Dimitrijević BB. Analysis of 5 non-coding region in hepatitis C virus by single-strand conformation polymorphism and low-stringency single specific primer PCR. in Clinical Chemistry and Laboratory Medicine. 2001;39(10):948-952.
doi:10.1515/CCLM.2001.152 .

Stamenković, Gorana, Guduric, J, Veličković, Zlate S., Skerl, Vesna, Krtolica-Žikić, Koviljka, Velikovic, E, Dimitrijević, Bogomir B., "Analysis of 5 non-coding region in hepatitis C virus by single-strand conformation polymorphism and low-stringency single specific primer PCR" in Clinical Chemistry and Laboratory Medicine, 39, no. 10 (2001):948-952,
https://doi.org/10.1515/CCLM.2001.152 . .

TY  - JOUR
AU  - Stamenković, Gorana
AU  - Zerjav, S
AU  - Velickovic, ZM
AU  - Krtolica-Žikić, Koviljka
AU  - Samardzija, VL
AU  - Jemuovic, L
AU  - Nozic, D
AU  - Dimitrijević, Bogomir B.
PY  - 2000
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2424
AB  - Blood samples from 190 patients that were anti-hepatitis C virus (HCV) positive were genotyped and 165 were found to contain HCV-RNA. Genotyping was performed by PCR based on type-specific primers (117 isolates) and LiPA test (48 isolates) and verifying by sequencing. In Serbia, the most frequent genotype was 1b (49.1%), followed by genotype 3 (21.2%) and genotype 1a (8.5%). The frequency of genotypes 2 and 4 was below 5% and mixed infections were encountered in 9.1% of cases. Distribution of genotypes was monitored in different risk groups: intravenous drug abusers, patients under blood transfusion, patients with previous history of surgery, patients undergoing hemodialysis and those with unknown risk factors. Genotype distribution is essentially the same in all the groups, except for the patients undergoing hemodialysis and those with previous history of surgery where significant difference exists compared with the group with unknown route of transmission (p LT 0.001 and p LT 0.05, respectively). There exists significant age-dependent genotype 3 distribution in Serbian population (p LT 0.01).
T2  - European Journal of Epidemiology
T1  - Distribution of HCV genotypes among risk groups in Serbia
VL  - 16
IS  - 10
SP  - 949
EP  - 954
DO  - 10.1023/A:1011060505152
ER  - 

@article{
author = "Stamenković, Gorana and Zerjav, S and Velickovic, ZM and Krtolica-Žikić, Koviljka and Samardzija, VL and Jemuovic, L and Nozic, D and Dimitrijević, Bogomir B.",
year = "2000",
abstract = "Blood samples from 190 patients that were anti-hepatitis C virus (HCV) positive were genotyped and 165 were found to contain HCV-RNA. Genotyping was performed by PCR based on type-specific primers (117 isolates) and LiPA test (48 isolates) and verifying by sequencing. In Serbia, the most frequent genotype was 1b (49.1%), followed by genotype 3 (21.2%) and genotype 1a (8.5%). The frequency of genotypes 2 and 4 was below 5% and mixed infections were encountered in 9.1% of cases. Distribution of genotypes was monitored in different risk groups: intravenous drug abusers, patients under blood transfusion, patients with previous history of surgery, patients undergoing hemodialysis and those with unknown risk factors. Genotype distribution is essentially the same in all the groups, except for the patients undergoing hemodialysis and those with previous history of surgery where significant difference exists compared with the group with unknown route of transmission (p LT 0.001 and p LT 0.05, respectively). There exists significant age-dependent genotype 3 distribution in Serbian population (p LT 0.01).",
journal = "European Journal of Epidemiology",
title = "Distribution of HCV genotypes among risk groups in Serbia",
volume = "16",
number = "10",
pages = "949-954",
doi = "10.1023/A:1011060505152"
}

Stamenković, G., Zerjav, S., Velickovic, Z., Krtolica-Žikić, K., Samardzija, V., Jemuovic, L., Nozic, D.,& Dimitrijević, B. B.. (2000). Distribution of HCV genotypes among risk groups in Serbia. in European Journal of Epidemiology, 16(10), 949-954.
https://doi.org/10.1023/A:1011060505152

Stamenković G, Zerjav S, Velickovic Z, Krtolica-Žikić K, Samardzija V, Jemuovic L, Nozic D, Dimitrijević BB. Distribution of HCV genotypes among risk groups in Serbia. in European Journal of Epidemiology. 2000;16(10):949-954.
doi:10.1023/A:1011060505152 .

Stamenković, Gorana, Zerjav, S, Velickovic, ZM, Krtolica-Žikić, Koviljka, Samardzija, VL, Jemuovic, L, Nozic, D, Dimitrijević, Bogomir B., "Distribution of HCV genotypes among risk groups in Serbia" in European Journal of Epidemiology, 16, no. 10 (2000):949-954,
https://doi.org/10.1023/A:1011060505152 . .