TY  - JOUR
AU  - Todorović, Jasna
AU  - Dinčić, Marko
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Nešović-Ostojić, Jelena
AU  - Kovačević, Sanjin
AU  - Lopičić, Srđan
AU  - Spasić, Svetolik
AU  - Brkić, Predrag
AU  - Milovanović, Aleksandar
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10745
AB  - Background: Thyroid dysfunctions as well as sleep abnormalities are usually followed by neurological, psychiatric and/or behavioral disorders. On the other hand, changes in the brain adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) activities show significant importance in pathogenetic pathways in the evolution of numerous neuropsychiatric diseases. Methods: This study aimed to evaluate the in vivo simultaneous effects of hypothyroidism and paradoxical sleep deprivation for 72 h on synaptosomalATPases and AChE activities of whole rat brains. In order to induce hypothyroidism, 6-n-propyl-2-thiouracil was administrated in drinking water during 21 days. The modified multiple platform method was used to induce paradoxical sleep deprivation. The AChE and ATPases activities were measured using spectrophotometric methods. Results: Hypothyroidism significantly increased the activity of Na+/K+-ATPase compared to other groups, while at the same time significantly decreased AChE activity compared to the CT and SD groups. Paradoxical sleep deprivation significantly increased AChE activity compared to other groups. The simultaneous effect of hypothyroidism and sleep deprivation reduced the activity of all three enzymes (for Na+/K+-ATPase between HT/SD and HT group p < 0.0001, SD group p < 0.001,CT group p = 0.013; for ecto-ATPases between HT/SD and HT group p = 0.0034, SD group p = 0.0001, CT group p = 0.0007; for AChE between HT/SD and HT group p < 0.05, SD group p < 0.0001, CT group p < 0.0001). Conclusions: The effect of simultaneous existence of hypothyroidism and paradoxical sleep deprivation reduces the activity of the Na+/K+-ATPase, ecto-ATPases, and AChE, what is different from individual effect of hypothyroidism and paradoxical sleep deprivation itself. This knowledge could help in the choice of appropriate therapy in such condition. © 2023 Elsevier B.V.
T2  - Sleep Medicine
T1  - The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain
VL  - 105
SP  - 14
EP  - 20
DO  - 10.1016/j.sleep.2023.03.002
ER  - 

@article{
author = "Todorović, Jasna and Dinčić, Marko and Krstić, Danijela Z. and Čolović, Mirjana B. and Nešović-Ostojić, Jelena and Kovačević, Sanjin and Lopičić, Srđan and Spasić, Svetolik and Brkić, Predrag and Milovanović, Aleksandar",
year = "2023",
abstract = "Background: Thyroid dysfunctions as well as sleep abnormalities are usually followed by neurological, psychiatric and/or behavioral disorders. On the other hand, changes in the brain adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) activities show significant importance in pathogenetic pathways in the evolution of numerous neuropsychiatric diseases. Methods: This study aimed to evaluate the in vivo simultaneous effects of hypothyroidism and paradoxical sleep deprivation for 72 h on synaptosomalATPases and AChE activities of whole rat brains. In order to induce hypothyroidism, 6-n-propyl-2-thiouracil was administrated in drinking water during 21 days. The modified multiple platform method was used to induce paradoxical sleep deprivation. The AChE and ATPases activities were measured using spectrophotometric methods. Results: Hypothyroidism significantly increased the activity of Na+/K+-ATPase compared to other groups, while at the same time significantly decreased AChE activity compared to the CT and SD groups. Paradoxical sleep deprivation significantly increased AChE activity compared to other groups. The simultaneous effect of hypothyroidism and sleep deprivation reduced the activity of all three enzymes (for Na+/K+-ATPase between HT/SD and HT group p < 0.0001, SD group p < 0.001,CT group p = 0.013; for ecto-ATPases between HT/SD and HT group p = 0.0034, SD group p = 0.0001, CT group p = 0.0007; for AChE between HT/SD and HT group p < 0.05, SD group p < 0.0001, CT group p < 0.0001). Conclusions: The effect of simultaneous existence of hypothyroidism and paradoxical sleep deprivation reduces the activity of the Na+/K+-ATPase, ecto-ATPases, and AChE, what is different from individual effect of hypothyroidism and paradoxical sleep deprivation itself. This knowledge could help in the choice of appropriate therapy in such condition. © 2023 Elsevier B.V.",
journal = "Sleep Medicine",
title = "The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain",
volume = "105",
pages = "14-20",
doi = "10.1016/j.sleep.2023.03.002"
}

Todorović, J., Dinčić, M., Krstić, D. Z., Čolović, M. B., Nešović-Ostojić, J., Kovačević, S., Lopičić, S., Spasić, S., Brkić, P.,& Milovanović, A.. (2023). The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain. in Sleep Medicine, 105, 14-20.
https://doi.org/10.1016/j.sleep.2023.03.002

Todorović J, Dinčić M, Krstić DZ, Čolović MB, Nešović-Ostojić J, Kovačević S, Lopičić S, Spasić S, Brkić P, Milovanović A. The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain. in Sleep Medicine. 2023;105:14-20.
doi:10.1016/j.sleep.2023.03.002 .

Todorović, Jasna, Dinčić, Marko, Krstić, Danijela Z., Čolović, Mirjana B., Nešović-Ostojić, Jelena, Kovačević, Sanjin, Lopičić, Srđan, Spasić, Svetolik, Brkić, Predrag, Milovanović, Aleksandar, "The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain" in Sleep Medicine, 105 (2023):14-20,
https://doi.org/10.1016/j.sleep.2023.03.002 . .

TY  - JOUR
AU  - Stojanović, Marko
AU  - Lalatović, Jovana
AU  - Milosavljević, Aleksandra
AU  - Savić, Nada
AU  - Simms, Charlotte
AU  - Radosavljević, Branimir
AU  - Ćetković, Mila
AU  - Kravić Stevović, Tamara
AU  - Mrda, Davor
AU  - Čolović, Mirjana B.
AU  - Parac-Vogt, Tatjana N.
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11091
AB  - In this study, we demonstrate for the first time, that a discrete metal-oxo cluster α-/β-K 6 P 2 W 18 O 62 (WD-POM) exhibits superior performance as a computed tomography (CT) contrast agent, in comparison to the standard contrast agent iohexol. A toxicity evaluation of WD-POM was performed according to standard toxicological protocols using Wistar albino rats. The maximum tolerable dose (MTD) of 2000 mg/kg was initially determined after oral WD-POM application. The acute intravenous toxicity of single WD-POM doses (1/3, 1/5, and 1/10 MTD), which are at least fifty times higher than the typically used dose (0.015 mmol W kg −1 ) of tungsten-based contrast agents, was evaluated for 14 days. The results of arterial blood gas analysis, CO-oximetry status, electrolyte and lactate levels for 1/10 MTD group (80% survival rate) indicated the mixed respiratory and metabolic acidosis. The highest deposition of WD-POM (0.6 ppm tungsten) was found in the kidney, followed by liver (0.15 ppm tungsten), for which the histological analysis revealed morphological irregularities, although the renal function parameters (creatinine and BUN levels) were within the physiological range. This study is the first and important step in evaluating side effects of polyoxometalate nanoclusters, which in recent years have shown a large potential as therapeutics and contrast agents.
T2  - Scientific Reports
T1  - In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography
VL  - 13
IS  - 1
SP  - 9140
DO  - 10.1038/s41598-023-36317-8
ER  - 

@article{
author = "Stojanović, Marko and Lalatović, Jovana and Milosavljević, Aleksandra and Savić, Nada and Simms, Charlotte and Radosavljević, Branimir and Ćetković, Mila and Kravić Stevović, Tamara and Mrda, Davor and Čolović, Mirjana B. and Parac-Vogt, Tatjana N. and Krstić, Danijela",
year = "2023",
abstract = "In this study, we demonstrate for the first time, that a discrete metal-oxo cluster α-/β-K 6 P 2 W 18 O 62 (WD-POM) exhibits superior performance as a computed tomography (CT) contrast agent, in comparison to the standard contrast agent iohexol. A toxicity evaluation of WD-POM was performed according to standard toxicological protocols using Wistar albino rats. The maximum tolerable dose (MTD) of 2000 mg/kg was initially determined after oral WD-POM application. The acute intravenous toxicity of single WD-POM doses (1/3, 1/5, and 1/10 MTD), which are at least fifty times higher than the typically used dose (0.015 mmol W kg −1 ) of tungsten-based contrast agents, was evaluated for 14 days. The results of arterial blood gas analysis, CO-oximetry status, electrolyte and lactate levels for 1/10 MTD group (80% survival rate) indicated the mixed respiratory and metabolic acidosis. The highest deposition of WD-POM (0.6 ppm tungsten) was found in the kidney, followed by liver (0.15 ppm tungsten), for which the histological analysis revealed morphological irregularities, although the renal function parameters (creatinine and BUN levels) were within the physiological range. This study is the first and important step in evaluating side effects of polyoxometalate nanoclusters, which in recent years have shown a large potential as therapeutics and contrast agents.",
journal = "Scientific Reports",
title = "In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography",
volume = "13",
number = "1",
pages = "9140",
doi = "10.1038/s41598-023-36317-8"
}

Stojanović, M., Lalatović, J., Milosavljević, A., Savić, N., Simms, C., Radosavljević, B., Ćetković, M., Kravić Stevović, T., Mrda, D., Čolović, M. B., Parac-Vogt, T. N.,& Krstić, D.. (2023). In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography. in Scientific Reports, 13(1), 9140.
https://doi.org/10.1038/s41598-023-36317-8

Stojanović M, Lalatović J, Milosavljević A, Savić N, Simms C, Radosavljević B, Ćetković M, Kravić Stevović T, Mrda D, Čolović MB, Parac-Vogt TN, Krstić D. In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography. in Scientific Reports. 2023;13(1):9140.
doi:10.1038/s41598-023-36317-8 .

Stojanović, Marko, Lalatović, Jovana, Milosavljević, Aleksandra, Savić, Nada, Simms, Charlotte, Radosavljević, Branimir, Ćetković, Mila, Kravić Stevović, Tamara, Mrda, Davor, Čolović, Mirjana B., Parac-Vogt, Tatjana N., Krstić, Danijela, "In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography" in Scientific Reports, 13, no. 1 (2023):9140,
https://doi.org/10.1038/s41598-023-36317-8 . .

TY  - CONF
AU  - Čakar, Uroš
AU  - Čolović, Mirjana
AU  - Čebela, Maria
AU  - Petrović, Aleksandar
AU  - Krstić, Danijela
AU  - Stanković, Ivan
AU  - Đorđević, Brižita
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11491
AB  - Natural active principles are present in the fruit and during its processing pass into the final products. Among many natural active principles it is important to emphasize those which exhibit beneficial health effect on human organism. Beneficial health effect of those compounds is result of antioxidant properties. Wine is fruit derived product which is rich source of those compounds. The aim of this study was to investigate the influence of technological process on the phenolic profile, antioxidant properties and activity of antioxidant protection enzymes in vitro. Fruit wines were produced in controlled conditions during microvinifications. Phenolic profile of fruit wines were obtained by UPLC MS/MS, while antioxidant properties and enzymatic activity determined by spectrophotometric methods. The content of phenolic compounds in fruit wines was significant. It is important to highlight that different applied technology during the production, influenced on the content of above mentioned compounds. Flavonoids and phenolic acids were the most important. Also fruit wines showed ability to activate enzymes of antioxidant protection, which could be used in the protection against free radicals and prevention of oxidative stress.
PB  - Belgrade : University of Belgrade, Institute for Multidisciplinary Research
C3  - Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade
T1  - The Influence of Technological Process on the Content of Natural Active Principles From Fruit Wines and Its Beneficial Health Effects
SP  - 47
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11491
ER  - 

@conference{
author = "Čakar, Uroš and Čolović, Mirjana and Čebela, Maria and Petrović, Aleksandar and Krstić, Danijela and Stanković, Ivan and Đorđević, Brižita",
year = "2023",
abstract = "Natural active principles are present in the fruit and during its processing pass into the final products. Among many natural active principles it is important to emphasize those which exhibit beneficial health effect on human organism. Beneficial health effect of those compounds is result of antioxidant properties. Wine is fruit derived product which is rich source of those compounds. The aim of this study was to investigate the influence of technological process on the phenolic profile, antioxidant properties and activity of antioxidant protection enzymes in vitro. Fruit wines were produced in controlled conditions during microvinifications. Phenolic profile of fruit wines were obtained by UPLC MS/MS, while antioxidant properties and enzymatic activity determined by spectrophotometric methods. The content of phenolic compounds in fruit wines was significant. It is important to highlight that different applied technology during the production, influenced on the content of above mentioned compounds. Flavonoids and phenolic acids were the most important. Also fruit wines showed ability to activate enzymes of antioxidant protection, which could be used in the protection against free radicals and prevention of oxidative stress.",
publisher = "Belgrade : University of Belgrade, Institute for Multidisciplinary Research",
journal = "Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade",
title = "The Influence of Technological Process on the Content of Natural Active Principles From Fruit Wines and Its Beneficial Health Effects",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11491"
}

Čakar, U., Čolović, M., Čebela, M., Petrović, A., Krstić, D., Stanković, I.,& Đorđević, B.. (2023). The Influence of Technological Process on the Content of Natural Active Principles From Fruit Wines and Its Beneficial Health Effects. in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade
Belgrade : University of Belgrade, Institute for Multidisciplinary Research., 47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11491

Čakar U, Čolović M, Čebela M, Petrović A, Krstić D, Stanković I, Đorđević B. The Influence of Technological Process on the Content of Natural Active Principles From Fruit Wines and Its Beneficial Health Effects. in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade. 2023;:47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11491 .

Čakar, Uroš, Čolović, Mirjana, Čebela, Maria, Petrović, Aleksandar, Krstić, Danijela, Stanković, Ivan, Đorđević, Brižita, "The Influence of Technological Process on the Content of Natural Active Principles From Fruit Wines and Its Beneficial Health Effects" in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade (2023):47-47,
https://hdl.handle.net/21.15107/rcub_vinar_11491 .

TY  - CONF
AU  - Čakar, Uroš
AU  - Čolović, Mirjana
AU  - Čebela, Maria
AU  - Petrović, Aleksandar
AU  - Krstić, Danijela
AU  - Stanković, Ivan
AU  - Đorđević, Brižita
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11507
AB  - Fruit and derived product represent a rich source of biologically active compounds which exhibit beneficial health effect on human organism. Among fruit especially berries it is important to highlight blueberries. One of derived products with added value from this fruit is wine. The aim of this study was to investigate in vitro activity of blueberry wine by monitoring activities of antioxidant protection enzymes and lipid peroxidation (malondialdehyde level) in isolated rat synaptosomes. Fruit wines were produced in controlled conditions of different microvinifications in which pure culture of selected wine yeast was used. Synaptosomes were isolated from the brain of Wistar albino rats. Analyzed wine samples influenced on the activity of antioxidant protection enzymes. Wine samples also showed ability to decrease malondialdehyde level. Activity for superoxide dismutase in synaptosomes was in range (6.47–7.21 U/mg) while catalase activity was (0.045–0.061 U/mg). Glutathione peroxidase activity was in range (0.0212– 0.0232 U/mg), as well as malondialdehyde level (2.17–2.35 nmol/mg). Obtained results indicate that blueberry wines possess antioxidant properties and abilities to protect against free radicals generated during oxidative stress.
PB  - University of Belgrade : Institute for Multidisciplinary Research
C3  - 6CSCS : 6th Serbian Ceramic Society Conference : programme and the book of abstracts; June 28-29; Belgrade
T1  - Blueberry wine biologically active compounds protect against oxidative stress
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11507
ER  - 

@conference{
author = "Čakar, Uroš and Čolović, Mirjana and Čebela, Maria and Petrović, Aleksandar and Krstić, Danijela and Stanković, Ivan and Đorđević, Brižita",
year = "2022",
abstract = "Fruit and derived product represent a rich source of biologically active compounds which exhibit beneficial health effect on human organism. Among fruit especially berries it is important to highlight blueberries. One of derived products with added value from this fruit is wine. The aim of this study was to investigate in vitro activity of blueberry wine by monitoring activities of antioxidant protection enzymes and lipid peroxidation (malondialdehyde level) in isolated rat synaptosomes. Fruit wines were produced in controlled conditions of different microvinifications in which pure culture of selected wine yeast was used. Synaptosomes were isolated from the brain of Wistar albino rats. Analyzed wine samples influenced on the activity of antioxidant protection enzymes. Wine samples also showed ability to decrease malondialdehyde level. Activity for superoxide dismutase in synaptosomes was in range (6.47–7.21 U/mg) while catalase activity was (0.045–0.061 U/mg). Glutathione peroxidase activity was in range (0.0212– 0.0232 U/mg), as well as malondialdehyde level (2.17–2.35 nmol/mg). Obtained results indicate that blueberry wines possess antioxidant properties and abilities to protect against free radicals generated during oxidative stress.",
publisher = "University of Belgrade : Institute for Multidisciplinary Research",
journal = "6CSCS : 6th Serbian Ceramic Society Conference : programme and the book of abstracts; June 28-29; Belgrade",
title = "Blueberry wine biologically active compounds protect against oxidative stress",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11507"
}

Čakar, U., Čolović, M., Čebela, M., Petrović, A., Krstić, D., Stanković, I.,& Đorđević, B.. (2022). Blueberry wine biologically active compounds protect against oxidative stress. in 6CSCS : 6th Serbian Ceramic Society Conference : programme and the book of abstracts; June 28-29; Belgrade
University of Belgrade : Institute for Multidisciplinary Research., 65.
https://hdl.handle.net/21.15107/rcub_vinar_11507

Čakar U, Čolović M, Čebela M, Petrović A, Krstić D, Stanković I, Đorđević B. Blueberry wine biologically active compounds protect against oxidative stress. in 6CSCS : 6th Serbian Ceramic Society Conference : programme and the book of abstracts; June 28-29; Belgrade. 2022;:65.
https://hdl.handle.net/21.15107/rcub_vinar_11507 .

Čakar, Uroš, Čolović, Mirjana, Čebela, Maria, Petrović, Aleksandar, Krstić, Danijela, Stanković, Ivan, Đorđević, Brižita, "Blueberry wine biologically active compounds protect against oxidative stress" in 6CSCS : 6th Serbian Ceramic Society Conference : programme and the book of abstracts; June 28-29; Belgrade (2022):65,
https://hdl.handle.net/21.15107/rcub_vinar_11507 .

TY  - JOUR
AU  - Čakar, Uroš
AU  - Čolović, Mirjana B.
AU  - Milenković, Danijela
AU  - Medić, Branislava
AU  - Krstić, Danijela Z.
AU  - Petrović, Aleksandar
AU  - Ðoređvić, Brižita
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9889
AB  - This study aimed to evaluate, in vitro, the antioxidative potential of fruit wines produced from berry fruits (i.e., black chokeberry, blueberry, blackberry, and raspberry), cherry, and apple by different technological processes. For this purpose, the activities of antioxidant enzymes (catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD)) and malondialdehyde (MDA) content as a marker of membrane damage were determined in wine-treated synaptosomes with hydrogen peroxide-induced oxidative stress. All studied wines induced increased antioxidant enzyme activities and decreased MDA levels compared to hydrogen peroxide-treated synaptosomes (i.e., control). The highest SOD activity was observed in synaptosomes treated with blackberry wine (6.81 U/mg), whereas blueberry wine induced the highest catalase and glutathione peroxidase activities (0.058 U/mg and 0.017 U/mg, respectively). Black chokeberry proved to be the best in lipid peroxidation protection with the lowest MDA value (1.42 nmol/mg). Finally, principal component analysis and hierarchical cluster analysis additionally highlighted a higher antioxidant capacity of wines produced from dark-skinned fruits (i.e., blackberry, black chokeberry, and blueberry). The results suggest protective effects of the fruit wines against oxidative damage, and, accordingly, their promising application as functional food.
T2  - Agronomy
T1  - Protective effects of fruit wines against hydrogen peroxide—induced oxidative stress in rat synaptosomes
VL  - 11
IS  - 7
DO  - 10.3390/agronomy11071414
ER  - 

@article{
author = "Čakar, Uroš and Čolović, Mirjana B. and Milenković, Danijela and Medić, Branislava and Krstić, Danijela Z. and Petrović, Aleksandar and Ðoređvić, Brižita",
year = "2021",
abstract = "This study aimed to evaluate, in vitro, the antioxidative potential of fruit wines produced from berry fruits (i.e., black chokeberry, blueberry, blackberry, and raspberry), cherry, and apple by different technological processes. For this purpose, the activities of antioxidant enzymes (catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD)) and malondialdehyde (MDA) content as a marker of membrane damage were determined in wine-treated synaptosomes with hydrogen peroxide-induced oxidative stress. All studied wines induced increased antioxidant enzyme activities and decreased MDA levels compared to hydrogen peroxide-treated synaptosomes (i.e., control). The highest SOD activity was observed in synaptosomes treated with blackberry wine (6.81 U/mg), whereas blueberry wine induced the highest catalase and glutathione peroxidase activities (0.058 U/mg and 0.017 U/mg, respectively). Black chokeberry proved to be the best in lipid peroxidation protection with the lowest MDA value (1.42 nmol/mg). Finally, principal component analysis and hierarchical cluster analysis additionally highlighted a higher antioxidant capacity of wines produced from dark-skinned fruits (i.e., blackberry, black chokeberry, and blueberry). The results suggest protective effects of the fruit wines against oxidative damage, and, accordingly, their promising application as functional food.",
journal = "Agronomy",
title = "Protective effects of fruit wines against hydrogen peroxide—induced oxidative stress in rat synaptosomes",
volume = "11",
number = "7",
doi = "10.3390/agronomy11071414"
}

Čakar, U., Čolović, M. B., Milenković, D., Medić, B., Krstić, D. Z., Petrović, A.,& Ðoređvić, B.. (2021). Protective effects of fruit wines against hydrogen peroxide—induced oxidative stress in rat synaptosomes. in Agronomy, 11(7).
https://doi.org/10.3390/agronomy11071414

Čakar U, Čolović MB, Milenković D, Medić B, Krstić DZ, Petrović A, Ðoređvić B. Protective effects of fruit wines against hydrogen peroxide—induced oxidative stress in rat synaptosomes. in Agronomy. 2021;11(7).
doi:10.3390/agronomy11071414 .

Čakar, Uroš, Čolović, Mirjana B., Milenković, Danijela, Medić, Branislava, Krstić, Danijela Z., Petrović, Aleksandar, Ðoređvić, Brižita, "Protective effects of fruit wines against hydrogen peroxide—induced oxidative stress in rat synaptosomes" in Agronomy, 11, no. 7 (2021),
https://doi.org/10.3390/agronomy11071414 . .

TY  - JOUR
AU  - Isaković, Anđelka M.
AU  - Čolović, Mirjana B.
AU  - Ma, Tian
AU  - Ma, Xiang
AU  - Jeremić, Marija
AU  - Gerić, Marko
AU  - Gajski, Goran
AU  - Misirlić-Denčić, Sonja
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9952
AB  - Polyoxo-noble-metalates (PONMs), a class of molecular noble metal-oxo nanoclusters that combine features of both polyoxometalates and noble metals, are a promising platform for the development of next-generation antitumor metallodrugs. This study aimed to evaluate the antitumor potential against human neuroblastoma cells (SH-SY5Y), as well as toxicity towards healthy human peripheral blood cells (HPBCs), of five polyoxopalladates(II): (Na8[Pd13As8O34(OH)6]·42H2O (Pd13), Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3]·2NaOAc·32H2O (SrPd12), Na6[Pd13(AsPh)8O32]·23H2O (Pd13L), Na12[SnO8Pd12(PO4)8]·43H2O (SnPd12), and Na12[PbO8Pd12(PO4)8]·38H2O (PbPd12)), as the largest subset of PONMs. A pure inorganic, Pd13, was found as the most potent and selective antineuroblastoma agent with IC50 values (µM) of 7.2 ± 2.2 and 4.4 ± 1.2 for 24 and 48 h treatment, respectively, even lower than cisplatin (28.4 ± 7.4 and 11.6 ± 0.8). The obtained IC50 values (µM) for 24/48 h treatment with SrPd12 and Pd13L were 75.8 ± 6.7/76.7 ± 22.9 and 63.8 ± 3.6/21.4 ± 10.8, respectively, whereas SnPd12 and PbPd12 did not remarkably affect the SH-SY5Y viability (IC50 > > 100 µM). Pd13 caused depolarisation of inner mitochondrial membrane prior to superoxide ion hyperproduction, followed by caspase activation, DNA fragmentation and cell cycle arrest, all hallmarks of apoptotic cell death, and accompanied by an increase in acidic vesicles content, suggestive of autophagy induction. Importantly, Pd13 demonstrated the antitumor effect at concentrations not cytogenotoxic for normal HPBCs. On the contrary, SrPd12 and Pd13L at concentrations ≥ 1/3 IC50 (24 h) decreased HPBC viability and increased % tail DNA up to 42% and 3.05 times, respectively, related to control. SnPd12 and PbPd12, previously confirmed promising antileukemic agents, did not exhibit cytogenotoxicity to HPBCs, and thus could be regarded as tumor cell specific and selective drug candidates.
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - Selected polyoxopalladates as promising and selective antitumor drug candidates
VL  - 26
IS  - 8
SP  - 957
EP  - 971
DO  - 10.1007/s00775-021-01905-4
ER  - 

@article{
author = "Isaković, Anđelka M. and Čolović, Mirjana B. and Ma, Tian and Ma, Xiang and Jeremić, Marija and Gerić, Marko and Gajski, Goran and Misirlić-Denčić, Sonja and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2021",
abstract = "Polyoxo-noble-metalates (PONMs), a class of molecular noble metal-oxo nanoclusters that combine features of both polyoxometalates and noble metals, are a promising platform for the development of next-generation antitumor metallodrugs. This study aimed to evaluate the antitumor potential against human neuroblastoma cells (SH-SY5Y), as well as toxicity towards healthy human peripheral blood cells (HPBCs), of five polyoxopalladates(II): (Na8[Pd13As8O34(OH)6]·42H2O (Pd13), Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3]·2NaOAc·32H2O (SrPd12), Na6[Pd13(AsPh)8O32]·23H2O (Pd13L), Na12[SnO8Pd12(PO4)8]·43H2O (SnPd12), and Na12[PbO8Pd12(PO4)8]·38H2O (PbPd12)), as the largest subset of PONMs. A pure inorganic, Pd13, was found as the most potent and selective antineuroblastoma agent with IC50 values (µM) of 7.2 ± 2.2 and 4.4 ± 1.2 for 24 and 48 h treatment, respectively, even lower than cisplatin (28.4 ± 7.4 and 11.6 ± 0.8). The obtained IC50 values (µM) for 24/48 h treatment with SrPd12 and Pd13L were 75.8 ± 6.7/76.7 ± 22.9 and 63.8 ± 3.6/21.4 ± 10.8, respectively, whereas SnPd12 and PbPd12 did not remarkably affect the SH-SY5Y viability (IC50 > > 100 µM). Pd13 caused depolarisation of inner mitochondrial membrane prior to superoxide ion hyperproduction, followed by caspase activation, DNA fragmentation and cell cycle arrest, all hallmarks of apoptotic cell death, and accompanied by an increase in acidic vesicles content, suggestive of autophagy induction. Importantly, Pd13 demonstrated the antitumor effect at concentrations not cytogenotoxic for normal HPBCs. On the contrary, SrPd12 and Pd13L at concentrations ≥ 1/3 IC50 (24 h) decreased HPBC viability and increased % tail DNA up to 42% and 3.05 times, respectively, related to control. SnPd12 and PbPd12, previously confirmed promising antileukemic agents, did not exhibit cytogenotoxicity to HPBCs, and thus could be regarded as tumor cell specific and selective drug candidates.",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "Selected polyoxopalladates as promising and selective antitumor drug candidates",
volume = "26",
number = "8",
pages = "957-971",
doi = "10.1007/s00775-021-01905-4"
}

Isaković, A. M., Čolović, M. B., Ma, T., Ma, X., Jeremić, M., Gerić, M., Gajski, G., Misirlić-Denčić, S., Kortz, U.,& Krstić, D. Z.. (2021). Selected polyoxopalladates as promising and selective antitumor drug candidates. in JBIC Journal of Biological Inorganic Chemistry, 26(8), 957-971.
https://doi.org/10.1007/s00775-021-01905-4

Isaković AM, Čolović MB, Ma T, Ma X, Jeremić M, Gerić M, Gajski G, Misirlić-Denčić S, Kortz U, Krstić DZ. Selected polyoxopalladates as promising and selective antitumor drug candidates. in JBIC Journal of Biological Inorganic Chemistry. 2021;26(8):957-971.
doi:10.1007/s00775-021-01905-4 .

Isaković, Anđelka M., Čolović, Mirjana B., Ma, Tian, Ma, Xiang, Jeremić, Marija, Gerić, Marko, Gajski, Goran, Misirlić-Denčić, Sonja, Kortz, Ulrich, Krstić, Danijela Z., "Selected polyoxopalladates as promising and selective antitumor drug candidates" in JBIC Journal of Biological Inorganic Chemistry, 26, no. 8 (2021):957-971,
https://doi.org/10.1007/s00775-021-01905-4 . .

TY  - CONF
AU  - Čakar, Uroš
AU  - Čolović, Mirjana B.
AU  - Čebela, Maria
AU  - Lisov, Nikolina
AU  - Petrović, Aleksandar
AU  - Krstić, Danijela
AU  - Đorđević, Brižita
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10791
AB  - Significant amount of natural active compounds are present in the fruit. Those compounds exhibit beneficial effect on the human health. Antioxidant properties are very important for health prevention. The aim of this study was to investigate natural active compounds from fruit wines and its activity on enzymes of antioxidant protection in vitro. Fruit wines were produced in controlled conditions during microvinifications. Phenolic profile of fruit wines were obtained by UPLC MS/MS, while enzymatic activity determined by spectrophotometric methods. Fruit wines showed significant content of phenolic compounds among them it is possible to emphasize phenolic acids. Also fruit wines influenced on the activity of enzymes of antioxidant protection which could be used in the prevention of the oxidative stress.
PB  - Belgrade : Serbian Ceramic Society
C3  - Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade
T1  - Natural active compounds in the prevention of oxidative stress
SP  - 50
EP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10791
ER  - 

@conference{
author = "Čakar, Uroš and Čolović, Mirjana B. and Čebela, Maria and Lisov, Nikolina and Petrović, Aleksandar and Krstić, Danijela and Đorđević, Brižita",
year = "2021",
abstract = "Significant amount of natural active compounds are present in the fruit. Those compounds exhibit beneficial effect on the human health. Antioxidant properties are very important for health prevention. The aim of this study was to investigate natural active compounds from fruit wines and its activity on enzymes of antioxidant protection in vitro. Fruit wines were produced in controlled conditions during microvinifications. Phenolic profile of fruit wines were obtained by UPLC MS/MS, while enzymatic activity determined by spectrophotometric methods. Fruit wines showed significant content of phenolic compounds among them it is possible to emphasize phenolic acids. Also fruit wines influenced on the activity of enzymes of antioxidant protection which could be used in the prevention of the oxidative stress.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade",
title = "Natural active compounds in the prevention of oxidative stress",
pages = "50-51",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10791"
}

Čakar, U., Čolović, M. B., Čebela, M., Lisov, N., Petrović, A., Krstić, D.,& Đorđević, B.. (2021). Natural active compounds in the prevention of oxidative stress. in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade
Belgrade : Serbian Ceramic Society., 50-51.
https://hdl.handle.net/21.15107/rcub_vinar_10791

Čakar U, Čolović MB, Čebela M, Lisov N, Petrović A, Krstić D, Đorđević B. Natural active compounds in the prevention of oxidative stress. in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade. 2021;:50-51.
https://hdl.handle.net/21.15107/rcub_vinar_10791 .

Čakar, Uroš, Čolović, Mirjana B., Čebela, Maria, Lisov, Nikolina, Petrović, Aleksandar, Krstić, Danijela, Đorđević, Brižita, "Natural active compounds in the prevention of oxidative stress" in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade (2021):50-51,
https://hdl.handle.net/21.15107/rcub_vinar_10791 .

TY  - CONF
AU  - Čakar, Uroš
AU  - Čolović, Mirjana B.
AU  - Čebela, Maria
AU  - Lisov, Nikolina
AU  - Petrović, Aleksandar
AU  - Krstić, Danijela
AU  - Đorđević, Brižita
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10832
AB  - Drupe fruit like peach are rich source of compounds which possess beneficial effect on human organism. One of many derived products is fruit wine which is also good source of above mentioned compounds. The aim of this study was to investigate in vitro activity of peach wines by monitoring activities of antioxidant protection enzymes and lipid peroxidation (malondialdehyde level) both in vitro. Fruit wines were produced in controlled conditions during microvinifications. Enzymatic activity determined by spectrophotometric methods. Analyzed wine samples showed significant activity on antioxidant protection enzymes and decreased malondialdehyde level. Results indicate that peach wines showed protective activity against free radicals.
PB  - Belgrade : Serbian Ceramic Society
C3  - Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade
T1  - Drupe fruit and derived products as a promising source of natural active compounds against oxidative stress
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10832
ER  - 

@conference{
author = "Čakar, Uroš and Čolović, Mirjana B. and Čebela, Maria and Lisov, Nikolina and Petrović, Aleksandar and Krstić, Danijela and Đorđević, Brižita",
year = "2021",
abstract = "Drupe fruit like peach are rich source of compounds which possess beneficial effect on human organism. One of many derived products is fruit wine which is also good source of above mentioned compounds. The aim of this study was to investigate in vitro activity of peach wines by monitoring activities of antioxidant protection enzymes and lipid peroxidation (malondialdehyde level) both in vitro. Fruit wines were produced in controlled conditions during microvinifications. Enzymatic activity determined by spectrophotometric methods. Analyzed wine samples showed significant activity on antioxidant protection enzymes and decreased malondialdehyde level. Results indicate that peach wines showed protective activity against free radicals.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade",
title = "Drupe fruit and derived products as a promising source of natural active compounds against oxidative stress",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10832"
}

Čakar, U., Čolović, M. B., Čebela, M., Lisov, N., Petrović, A., Krstić, D.,& Đorđević, B.. (2021). Drupe fruit and derived products as a promising source of natural active compounds against oxidative stress. in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade
Belgrade : Serbian Ceramic Society., 72.
https://hdl.handle.net/21.15107/rcub_vinar_10832

Čakar U, Čolović MB, Čebela M, Lisov N, Petrović A, Krstić D, Đorđević B. Drupe fruit and derived products as a promising source of natural active compounds against oxidative stress. in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade. 2021;:72.
https://hdl.handle.net/21.15107/rcub_vinar_10832 .

Čakar, Uroš, Čolović, Mirjana B., Čebela, Maria, Lisov, Nikolina, Petrović, Aleksandar, Krstić, Danijela, Đorđević, Brižita, "Drupe fruit and derived products as a promising source of natural active compounds against oxidative stress" in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade (2021):72,
https://hdl.handle.net/21.15107/rcub_vinar_10832 .

TY  - CONF
AU  - Leskovac, Andreja
AU  - Čolović, Mirjana
AU  - Bondžić, Aleksandra
AU  - Petrović, Sandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11686
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
T1  - N-Acetylcysteine as Regulator of the Cellular Homeostasis
SP  - 192
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11686
ER  - 

@conference{
author = "Leskovac, Andreja and Čolović, Mirjana and Bondžić, Aleksandra and Petrović, Sandra",
year = "2021",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry",
title = "N-Acetylcysteine as Regulator of the Cellular Homeostasis",
pages = "192-195",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11686"
}

Leskovac, A., Čolović, M., Bondžić, A.,& Petrović, S.. (2021). N-Acetylcysteine as Regulator of the Cellular Homeostasis. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
Belgrade : Vinča Institute of Nuclear Sciences., 192-195.
https://hdl.handle.net/21.15107/rcub_vinar_11686

Leskovac A, Čolović M, Bondžić A, Petrović S. N-Acetylcysteine as Regulator of the Cellular Homeostasis. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry. 2021;:192-195.
https://hdl.handle.net/21.15107/rcub_vinar_11686 .

Leskovac, Andreja, Čolović, Mirjana, Bondžić, Aleksandra, Petrović, Sandra, "N-Acetylcysteine as Regulator of the Cellular Homeostasis" in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry (2021):192-195,
https://hdl.handle.net/21.15107/rcub_vinar_11686 .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Leskovac, Andreja
AU  - Vujačić Nikezić, Ana V.
AU  - Krstić, Danijela
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11688
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
T1  - In Vitro Evaluation of Chlorpyrifos Cytotoxic Effects
SP  - 135
EP  - 138
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11688
ER  - 

@conference{
author = "Čolović, Mirjana and Leskovac, Andreja and Vujačić Nikezić, Ana V. and Krstić, Danijela",
year = "2021",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry",
title = "In Vitro Evaluation of Chlorpyrifos Cytotoxic Effects",
pages = "135-138",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11688"
}

Čolović, M., Leskovac, A., Vujačić Nikezić, A. V.,& Krstić, D.. (2021). In Vitro Evaluation of Chlorpyrifos Cytotoxic Effects. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
Belgrade : Vinča Institute of Nuclear Sciences., 135-138.
https://hdl.handle.net/21.15107/rcub_vinar_11688

Čolović M, Leskovac A, Vujačić Nikezić AV, Krstić D. In Vitro Evaluation of Chlorpyrifos Cytotoxic Effects. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry. 2021;:135-138.
https://hdl.handle.net/21.15107/rcub_vinar_11688 .

Čolović, Mirjana, Leskovac, Andreja, Vujačić Nikezić, Ana V., Krstić, Danijela, "In Vitro Evaluation of Chlorpyrifos Cytotoxic Effects" in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry (2021):135-138,
https://hdl.handle.net/21.15107/rcub_vinar_11688 .

TY  - CONF
AU  - Petrović, Sandra
AU  - Vujačić Nikezić, Ana V.
AU  - Čolović, Mirjana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11689
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
T1  - Effect of Chlorpyrifos-oxon on Membrane Damage and Cell Viability
SP  - 139
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11689
ER  - 

@conference{
author = "Petrović, Sandra and Vujačić Nikezić, Ana V. and Čolović, Mirjana",
year = "2021",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry",
title = "Effect of Chlorpyrifos-oxon on Membrane Damage and Cell Viability",
pages = "139-142",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11689"
}

Petrović, S., Vujačić Nikezić, A. V.,& Čolović, M.. (2021). Effect of Chlorpyrifos-oxon on Membrane Damage and Cell Viability. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
Belgrade : Vinča Institute of Nuclear Sciences., 139-142.
https://hdl.handle.net/21.15107/rcub_vinar_11689

Petrović S, Vujačić Nikezić AV, Čolović M. Effect of Chlorpyrifos-oxon on Membrane Damage and Cell Viability. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry. 2021;:139-142.
https://hdl.handle.net/21.15107/rcub_vinar_11689 .

Petrović, Sandra, Vujačić Nikezić, Ana V., Čolović, Mirjana, "Effect of Chlorpyrifos-oxon on Membrane Damage and Cell Viability" in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry (2021):139-142,
https://hdl.handle.net/21.15107/rcub_vinar_11689 .

TY  - JOUR
AU  - Dinčić, Marko
AU  - Čolović, Mirjana B.
AU  - Sarić Matutinović, Marija
AU  - Ćetković, Mila
AU  - Kravić-Stevović, Tamara K.
AU  - Mougharbel, Ali S.
AU  - Todorović, Jasna
AU  - Ignjatović, Svetlana
AU  - Radosavljević, Branimir
AU  - Milisavljević, Milan
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8479
AB  - In this study, thein vivohypoglycemic effect of a donut-shaped polyanion salt (NH4)14[Na@P5W30O110]$31H2O{NaP5W30} and its Ag-containing derivative K14[Ag@P5W30O110]$22H2O$6KCl {AgP5W30}, as wellas their hepatotoxicity and nephrotoxicity, was evaluated. In the screening hypoglycemic study,Wistaralbinorats with streptozotocin induced diabetes were treated intraperitoneally with three single doses (5,10, and 20 mg per kg per b.w.) of both investigated polyoxotungstates. The blood glucose levels,measured before and after 2, 4 and 6 h polyoxotungstate application, showed that both studiedcompounds induced the most pronounced and time dependent glucose lowering effects at the doses of20 mg kg1. Thus, daily doses of 20 mg kg1were administered toWistar albinorats orally for 14 days infurther toxicity examinations. The serum glucose concentration and biochemical parameters of kidneyand liver function, as well as a histopathological analysis of kidney and liver tissues were evaluated 14days after the polyoxotungstate administration. Both investigated compounds did not induce statisticallysignificant alterations of the serum glucose and uric acid concentrations, as well as some of the liverfunction markers (serum alanine and aspartate aminotransferases, and alkaline phosphatase activities).However, the significant decrease in serum total protein and albumin concentrations and the increase inbiochemical parameters of renal function–serum urea (up to 63.1%) and creatinine concentrations (upto 23.3%) were observed for both polyoxotungstates. In addition, the detected biochemical changeswere in accordance with kidney and liver histhopathological analysis. Accordingly, the hepatotoxic andnephrotoxic effects of these potential antidiabetic polyoxotungstates could be considered as mild.
T2  - RSC Advances
T1  - In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system
VL  - 10
IS  - 5
SP  - 2846
EP  - 2855
DO  - 10.1039/C9RA09790B
ER  - 

@article{
author = "Dinčić, Marko and Čolović, Mirjana B. and Sarić Matutinović, Marija and Ćetković, Mila and Kravić-Stevović, Tamara K. and Mougharbel, Ali S. and Todorović, Jasna and Ignjatović, Svetlana and Radosavljević, Branimir and Milisavljević, Milan and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2020",
abstract = "In this study, thein vivohypoglycemic effect of a donut-shaped polyanion salt (NH4)14[Na@P5W30O110]$31H2O{NaP5W30} and its Ag-containing derivative K14[Ag@P5W30O110]$22H2O$6KCl {AgP5W30}, as wellas their hepatotoxicity and nephrotoxicity, was evaluated. In the screening hypoglycemic study,Wistaralbinorats with streptozotocin induced diabetes were treated intraperitoneally with three single doses (5,10, and 20 mg per kg per b.w.) of both investigated polyoxotungstates. The blood glucose levels,measured before and after 2, 4 and 6 h polyoxotungstate application, showed that both studiedcompounds induced the most pronounced and time dependent glucose lowering effects at the doses of20 mg kg1. Thus, daily doses of 20 mg kg1were administered toWistar albinorats orally for 14 days infurther toxicity examinations. The serum glucose concentration and biochemical parameters of kidneyand liver function, as well as a histopathological analysis of kidney and liver tissues were evaluated 14days after the polyoxotungstate administration. Both investigated compounds did not induce statisticallysignificant alterations of the serum glucose and uric acid concentrations, as well as some of the liverfunction markers (serum alanine and aspartate aminotransferases, and alkaline phosphatase activities).However, the significant decrease in serum total protein and albumin concentrations and the increase inbiochemical parameters of renal function–serum urea (up to 63.1%) and creatinine concentrations (upto 23.3%) were observed for both polyoxotungstates. In addition, the detected biochemical changeswere in accordance with kidney and liver histhopathological analysis. Accordingly, the hepatotoxic andnephrotoxic effects of these potential antidiabetic polyoxotungstates could be considered as mild.",
journal = "RSC Advances",
title = "In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system",
volume = "10",
number = "5",
pages = "2846-2855",
doi = "10.1039/C9RA09790B"
}

Dinčić, M., Čolović, M. B., Sarić Matutinović, M., Ćetković, M., Kravić-Stevović, T. K., Mougharbel, A. S., Todorović, J., Ignjatović, S., Radosavljević, B., Milisavljević, M., Kortz, U.,& Krstić, D. Z.. (2020). In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system. in RSC Advances, 10(5), 2846-2855.
https://doi.org/10.1039/C9RA09790B

Dinčić M, Čolović MB, Sarić Matutinović M, Ćetković M, Kravić-Stevović TK, Mougharbel AS, Todorović J, Ignjatović S, Radosavljević B, Milisavljević M, Kortz U, Krstić DZ. In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system. in RSC Advances. 2020;10(5):2846-2855.
doi:10.1039/C9RA09790B .

Dinčić, Marko, Čolović, Mirjana B., Sarić Matutinović, Marija, Ćetković, Mila, Kravić-Stevović, Tamara K., Mougharbel, Ali S., Todorović, Jasna, Ignjatović, Svetlana, Radosavljević, Branimir, Milisavljević, Milan, Kortz, Ulrich, Krstić, Danijela Z., "In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system" in RSC Advances, 10, no. 5 (2020):2846-2855,
https://doi.org/10.1039/C9RA09790B . .

TY  - JOUR
AU  - Medić, Branislava
AU  - Stojanović, Marko
AU  - Stimec, Bojan V.
AU  - Divac, Nevena
AU  - Savić Vujović, Katarina
AU  - Stojanović, Radan
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Prostran, Milica
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8838
AB  - Lithium is the smallest monovalent cation with many different biological effects. Although lithium is present in the pharmacotherapy of psychiatric illnesses for decades, its precise mechanism of action is still not clarified. Today lithium represents first-line therapy for bipolar disorders (because it possesses both antimanic and antidepressant properties) and the adjunctive treatment for major depression (due to its antisuicidal effects). Beside, lithium showed some protective effects in neurological diseases including acute neural injury, chronic degenerative conditions, Alzheimer's disease as well as in treating leucopenia, hepatitis and some renal diseases. Recent evidence suggested that lithium also possesses some anticancer properties due to its inhibition of Glycogen Synthase Kinase 3 beta (GSK3β) which is included in the regulation of a lot of important cellular processes such as: glycogen metabolism, inflammation, immunomodulation, apoptosis, tissue injury, regeneration etc. Although recent evidence suggested a potential utility of lithium in different conditions, its broader use in clinical practice still trails. The reason for this is a narrow therapeutic index of lithium, numerous toxic effects in various organ systems and some clinically relevant interactions with other drugs. Additionally, it is necessary to perform more preclinical as well as clinical studies in order to a precise therapeutic range of lithium, as well as its detailed mechanism of action. The aim of this review is to summarize the current knowledge concerning the pharmacological and toxicological effects of lithium. © 2020 Bentham Science Publishers.
T2  - Current Medicinal Chemistry
T1  - Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art
VL  - 27
IS  - 3
SP  - 337
EP  - 351
DO  - 10.2174/0929867325666180904124733
ER  - 

@article{
author = "Medić, Branislava and Stojanović, Marko and Stimec, Bojan V. and Divac, Nevena and Savić Vujović, Katarina and Stojanović, Radan and Čolović, Mirjana B. and Krstić, Danijela Z. and Prostran, Milica",
year = "2020",
abstract = "Lithium is the smallest monovalent cation with many different biological effects. Although lithium is present in the pharmacotherapy of psychiatric illnesses for decades, its precise mechanism of action is still not clarified. Today lithium represents first-line therapy for bipolar disorders (because it possesses both antimanic and antidepressant properties) and the adjunctive treatment for major depression (due to its antisuicidal effects). Beside, lithium showed some protective effects in neurological diseases including acute neural injury, chronic degenerative conditions, Alzheimer's disease as well as in treating leucopenia, hepatitis and some renal diseases. Recent evidence suggested that lithium also possesses some anticancer properties due to its inhibition of Glycogen Synthase Kinase 3 beta (GSK3β) which is included in the regulation of a lot of important cellular processes such as: glycogen metabolism, inflammation, immunomodulation, apoptosis, tissue injury, regeneration etc. Although recent evidence suggested a potential utility of lithium in different conditions, its broader use in clinical practice still trails. The reason for this is a narrow therapeutic index of lithium, numerous toxic effects in various organ systems and some clinically relevant interactions with other drugs. Additionally, it is necessary to perform more preclinical as well as clinical studies in order to a precise therapeutic range of lithium, as well as its detailed mechanism of action. The aim of this review is to summarize the current knowledge concerning the pharmacological and toxicological effects of lithium. © 2020 Bentham Science Publishers.",
journal = "Current Medicinal Chemistry",
title = "Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art",
volume = "27",
number = "3",
pages = "337-351",
doi = "10.2174/0929867325666180904124733"
}

Medić, B., Stojanović, M., Stimec, B. V., Divac, N., Savić Vujović, K., Stojanović, R., Čolović, M. B., Krstić, D. Z.,& Prostran, M.. (2020). Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art. in Current Medicinal Chemistry, 27(3), 337-351.
https://doi.org/10.2174/0929867325666180904124733

Medić B, Stojanović M, Stimec BV, Divac N, Savić Vujović K, Stojanović R, Čolović MB, Krstić DZ, Prostran M. Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art. in Current Medicinal Chemistry. 2020;27(3):337-351.
doi:10.2174/0929867325666180904124733 .

Medić, Branislava, Stojanović, Marko, Stimec, Bojan V., Divac, Nevena, Savić Vujović, Katarina, Stojanović, Radan, Čolović, Mirjana B., Krstić, Danijela Z., Prostran, Milica, "Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art" in Current Medicinal Chemistry, 27, no. 3 (2020):337-351,
https://doi.org/10.2174/0929867325666180904124733 . .

TY  - JOUR
AU  - Van Gool, Alain
AU  - Corrales, Fernado
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Oliver-Martos, Begona
AU  - Martínez-Cáceres, Eva
AU  - Jakasa, Ivone
AU  - Gajski, Goran
AU  - Brun, Virginie
AU  - Kyriacou, Kyriacos
AU  - Burzynska-Pedziwiatr, Izabela
AU  - Wozniak, Lucyna Alicja
AU  - Nierkens, Stephan
AU  - Pascual García, César
AU  - Katrlik, Jaroslav
AU  - Bojić-Trbojević, Žanka
AU  - Vacek, Jan
AU  - Llorente, Alicia
AU  - Antohe, Felicia
AU  - Suica, Viorel
AU  - Suarez, Guillaume
AU  - T’Kindt, Ruben
AU  - Martin, Petra
AU  - Penque, Deborah
AU  - Martins, Ines Lanca
AU  - Bodoki, Ede
AU  - Iacob, Bogdan-Cezar
AU  - Aydindogan, Eda
AU  - Timur, Suna
AU  - Allinson, John
AU  - Sutton, Christopher
AU  - Luider, Theo
AU  - Wittfooth, Saara
AU  - Sammar, Marei
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9018
AB  - Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.
T2  - Expert Review of Proteomics
T1  - Analytical techniques for multiplex analysis of protein biomarkers
VL  - 17
IS  - 4
SP  - 257
EP  - 273
DO  - 10.1080/14789450.2020.1763174
ER  - 

@article{
author = "Van Gool, Alain and Corrales, Fernado and Čolović, Mirjana B. and Krstić, Danijela Z. and Oliver-Martos, Begona and Martínez-Cáceres, Eva and Jakasa, Ivone and Gajski, Goran and Brun, Virginie and Kyriacou, Kyriacos and Burzynska-Pedziwiatr, Izabela and Wozniak, Lucyna Alicja and Nierkens, Stephan and Pascual García, César and Katrlik, Jaroslav and Bojić-Trbojević, Žanka and Vacek, Jan and Llorente, Alicia and Antohe, Felicia and Suica, Viorel and Suarez, Guillaume and T’Kindt, Ruben and Martin, Petra and Penque, Deborah and Martins, Ines Lanca and Bodoki, Ede and Iacob, Bogdan-Cezar and Aydindogan, Eda and Timur, Suna and Allinson, John and Sutton, Christopher and Luider, Theo and Wittfooth, Saara and Sammar, Marei",
year = "2020",
abstract = "Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.",
journal = "Expert Review of Proteomics",
title = "Analytical techniques for multiplex analysis of protein biomarkers",
volume = "17",
number = "4",
pages = "257-273",
doi = "10.1080/14789450.2020.1763174"
}

Van Gool, A., Corrales, F., Čolović, M. B., Krstić, D. Z., Oliver-Martos, B., Martínez-Cáceres, E., Jakasa, I., Gajski, G., Brun, V., Kyriacou, K., Burzynska-Pedziwiatr, I., Wozniak, L. A., Nierkens, S., Pascual García, C., Katrlik, J., Bojić-Trbojević, Ž., Vacek, J., Llorente, A., Antohe, F., Suica, V., Suarez, G., T’Kindt, R., Martin, P., Penque, D., Martins, I. L., Bodoki, E., Iacob, B., Aydindogan, E., Timur, S., Allinson, J., Sutton, C., Luider, T., Wittfooth, S.,& Sammar, M.. (2020). Analytical techniques for multiplex analysis of protein biomarkers. in Expert Review of Proteomics, 17(4), 257-273.
https://doi.org/10.1080/14789450.2020.1763174

Van Gool A, Corrales F, Čolović MB, Krstić DZ, Oliver-Martos B, Martínez-Cáceres E, Jakasa I, Gajski G, Brun V, Kyriacou K, Burzynska-Pedziwiatr I, Wozniak LA, Nierkens S, Pascual García C, Katrlik J, Bojić-Trbojević Ž, Vacek J, Llorente A, Antohe F, Suica V, Suarez G, T’Kindt R, Martin P, Penque D, Martins IL, Bodoki E, Iacob B, Aydindogan E, Timur S, Allinson J, Sutton C, Luider T, Wittfooth S, Sammar M. Analytical techniques for multiplex analysis of protein biomarkers. in Expert Review of Proteomics. 2020;17(4):257-273.
doi:10.1080/14789450.2020.1763174 .

Van Gool, Alain, Corrales, Fernado, Čolović, Mirjana B., Krstić, Danijela Z., Oliver-Martos, Begona, Martínez-Cáceres, Eva, Jakasa, Ivone, Gajski, Goran, Brun, Virginie, Kyriacou, Kyriacos, Burzynska-Pedziwiatr, Izabela, Wozniak, Lucyna Alicja, Nierkens, Stephan, Pascual García, César, Katrlik, Jaroslav, Bojić-Trbojević, Žanka, Vacek, Jan, Llorente, Alicia, Antohe, Felicia, Suica, Viorel, Suarez, Guillaume, T’Kindt, Ruben, Martin, Petra, Penque, Deborah, Martins, Ines Lanca, Bodoki, Ede, Iacob, Bogdan-Cezar, Aydindogan, Eda, Timur, Suna, Allinson, John, Sutton, Christopher, Luider, Theo, Wittfooth, Saara, Sammar, Marei, "Analytical techniques for multiplex analysis of protein biomarkers" in Expert Review of Proteomics, 17, no. 4 (2020):257-273,
https://doi.org/10.1080/14789450.2020.1763174 . .

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Lazarević-Pašti, Tamara
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Čolović, Mirjana B.
AU  - Parac-Vogt, Tatjana N.
AU  - Janjić, Goran V.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9028
AB  - Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors,12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate(POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism andtryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significantchanges in the secondary structure of the enzyme. The molecular docking approach has revealed a newallosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChEby POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is consideredresponsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selectedPOMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. Itwas shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, whichindicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable ofbinding to an allosteric site that so far has not been considered responsible for enzyme inhibition could befundamental for the development of new drug design strategies and the discovery of more efficient AChEmodulators.
T2  - European Journal of Pharmaceutical Sciences
T1  - A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition
VL  - 151
SP  - 105376
DO  - 10.1016/j.ejps.2020.105376
ER  - 

@article{
author = "Bondžić, Aleksandra M. and Lazarević-Pašti, Tamara and Leskovac, Andreja and Petrović, Sandra and Čolović, Mirjana B. and Parac-Vogt, Tatjana N. and Janjić, Goran V.",
year = "2020",
abstract = "Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors,12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate(POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism andtryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significantchanges in the secondary structure of the enzyme. The molecular docking approach has revealed a newallosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChEby POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is consideredresponsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selectedPOMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. Itwas shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, whichindicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable ofbinding to an allosteric site that so far has not been considered responsible for enzyme inhibition could befundamental for the development of new drug design strategies and the discovery of more efficient AChEmodulators.",
journal = "European Journal of Pharmaceutical Sciences",
title = "A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition",
volume = "151",
pages = "105376",
doi = "10.1016/j.ejps.2020.105376"
}

Bondžić, A. M., Lazarević-Pašti, T., Leskovac, A., Petrović, S., Čolović, M. B., Parac-Vogt, T. N.,& Janjić, G. V.. (2020). A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition. in European Journal of Pharmaceutical Sciences, 151, 105376.
https://doi.org/10.1016/j.ejps.2020.105376

Bondžić AM, Lazarević-Pašti T, Leskovac A, Petrović S, Čolović MB, Parac-Vogt TN, Janjić GV. A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition. in European Journal of Pharmaceutical Sciences. 2020;151:105376.
doi:10.1016/j.ejps.2020.105376 .

Bondžić, Aleksandra M., Lazarević-Pašti, Tamara, Leskovac, Andreja, Petrović, Sandra, Čolović, Mirjana B., Parac-Vogt, Tatjana N., Janjić, Goran V., "A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition" in European Journal of Pharmaceutical Sciences, 151 (2020):105376,
https://doi.org/10.1016/j.ejps.2020.105376 . .

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Lacković, Milan
AU  - Lalatović, Jovana
AU  - Mougharbel, Ali S.
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10139
AB  - Background: Polyoxometalates (POMs) are negatively charged metal-oxo clusters of early transition metal ions in high oxidation states (e.g., WVI, MoVI, VV). POMs are of interest in the fields of catalysis, electronics, magnetic materials and nanotechnology. Moreover, POMs were shown to exhibit biological activities in vitro and in vivo, such as antitumor, antimicrobial, and antidiabetic. Methods: The literature search for this peer-reviewed article was performed using PubMed and Scopus databases with the help of appropriate keywords. Results: This review gives a comprehensive overview of recent studies regarding biological activities of polyoxometalates, and their biomedical applications as promising anti-viral, anti-bacterial, anti-tumor, and anti-diabetic agents. Additionally, their putative mechanisms of action and molecular targets are particularly considered. Conclusion: Although a wide range of biological activities of Polyoxometalates (POMs) has been reported, they are to the best of our knowledge not close to a clinical trial or a final application in the treatment of diabetes or infectious and malignant diseases. Accordingly, further studies should be directed towards determining the mechanism of POM biological actions, which would enable fine-tuning at the molecular level, and consequently efficient action towards biological targets and as low toxicity as possible. Furthermore, biomedical studies should be performed on solution-stable POMs employing physiological conditions and concentrations.
T2  - Current Medicinal Chemistry
T1  - Polyoxometalates in Biomedicine: Update and Overview
VL  - 27
IS  - 3
SP  - 362
EP  - 379
DO  - 10.2174/0929867326666190827153532
ER  - 

@article{
author = "Čolović, Mirjana B. and Lacković, Milan and Lalatović, Jovana and Mougharbel, Ali S. and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2020",
abstract = "Background: Polyoxometalates (POMs) are negatively charged metal-oxo clusters of early transition metal ions in high oxidation states (e.g., WVI, MoVI, VV). POMs are of interest in the fields of catalysis, electronics, magnetic materials and nanotechnology. Moreover, POMs were shown to exhibit biological activities in vitro and in vivo, such as antitumor, antimicrobial, and antidiabetic. Methods: The literature search for this peer-reviewed article was performed using PubMed and Scopus databases with the help of appropriate keywords. Results: This review gives a comprehensive overview of recent studies regarding biological activities of polyoxometalates, and their biomedical applications as promising anti-viral, anti-bacterial, anti-tumor, and anti-diabetic agents. Additionally, their putative mechanisms of action and molecular targets are particularly considered. Conclusion: Although a wide range of biological activities of Polyoxometalates (POMs) has been reported, they are to the best of our knowledge not close to a clinical trial or a final application in the treatment of diabetes or infectious and malignant diseases. Accordingly, further studies should be directed towards determining the mechanism of POM biological actions, which would enable fine-tuning at the molecular level, and consequently efficient action towards biological targets and as low toxicity as possible. Furthermore, biomedical studies should be performed on solution-stable POMs employing physiological conditions and concentrations.",
journal = "Current Medicinal Chemistry",
title = "Polyoxometalates in Biomedicine: Update and Overview",
volume = "27",
number = "3",
pages = "362-379",
doi = "10.2174/0929867326666190827153532"
}

Čolović, M. B., Lacković, M., Lalatović, J., Mougharbel, A. S., Kortz, U.,& Krstić, D. Z.. (2020). Polyoxometalates in Biomedicine: Update and Overview. in Current Medicinal Chemistry, 27(3), 362-379.
https://doi.org/10.2174/0929867326666190827153532

Čolović MB, Lacković M, Lalatović J, Mougharbel AS, Kortz U, Krstić DZ. Polyoxometalates in Biomedicine: Update and Overview. in Current Medicinal Chemistry. 2020;27(3):362-379.
doi:10.2174/0929867326666190827153532 .

Čolović, Mirjana B., Lacković, Milan, Lalatović, Jovana, Mougharbel, Ali S., Kortz, Ulrich, Krstić, Danijela Z., "Polyoxometalates in Biomedicine: Update and Overview" in Current Medicinal Chemistry, 27, no. 3 (2020):362-379,
https://doi.org/10.2174/0929867326666190827153532 . .

TY  - JOUR
AU  - Bošnjaković-Pavlović, Nada
AU  - Xu, Xiao
AU  - Krstić, Danijela Z.
AU  - Gillet, Jean-Michel
AU  - Wei, Yongge
AU  - Wu, Pingfan
AU  - Čolović, Mirjana B.
AU  - Spasojević-de Bire, Anne
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0162013418306998
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8355
AB  - The influence of three functionalized hexavanadates (V6): Na2 [V6O13{(OCH2)3CCH3}2], [H2]2 [V6O13{(OCH2)3CCH2OCOCH2CH3}2] and [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2 on Na+/K+-ATPase activity, was investigated in vitro. Including compounds already tested by Xu et al. (Journal of Inorganic Biochemistry 161 (2016) 27–36), all functionalized hexavanadates inhibit the activity of Na+/K+-ATPase in a dose-dependent manner but with different inhibitory potencies. Na2 [V6O13{(OCH2)3CCH3}2] was found to have the best inhibition properties - showing 50% inhibition IC50 = 5.50 × 10−5 M, while [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2] showed the lowest inhibitory power, IC50 = 1.31 × 10−4 M. In order to understand the bioactivity of functionalized hexavanadates series, we have also used a combined theoretical approach: determination of electrostatic potential from ab initio theoretical calculations and computation of the molecular interaction field (MIF) surface. © 2019
T2  - Journal of Inorganic Biochemistry
T1  - Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays
VL  - 198
SP  - 110720
DO  - 10.1016/j.jinorgbio.2019.110720
ER  - 

@article{
author = "Bošnjaković-Pavlović, Nada and Xu, Xiao and Krstić, Danijela Z. and Gillet, Jean-Michel and Wei, Yongge and Wu, Pingfan and Čolović, Mirjana B. and Spasojević-de Bire, Anne",
year = "2019",
abstract = "The influence of three functionalized hexavanadates (V6): Na2 [V6O13{(OCH2)3CCH3}2], [H2]2 [V6O13{(OCH2)3CCH2OCOCH2CH3}2] and [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2 on Na+/K+-ATPase activity, was investigated in vitro. Including compounds already tested by Xu et al. (Journal of Inorganic Biochemistry 161 (2016) 27–36), all functionalized hexavanadates inhibit the activity of Na+/K+-ATPase in a dose-dependent manner but with different inhibitory potencies. Na2 [V6O13{(OCH2)3CCH3}2] was found to have the best inhibition properties - showing 50% inhibition IC50 = 5.50 × 10−5 M, while [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2] showed the lowest inhibitory power, IC50 = 1.31 × 10−4 M. In order to understand the bioactivity of functionalized hexavanadates series, we have also used a combined theoretical approach: determination of electrostatic potential from ab initio theoretical calculations and computation of the molecular interaction field (MIF) surface. © 2019",
journal = "Journal of Inorganic Biochemistry",
title = "Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays",
volume = "198",
pages = "110720",
doi = "10.1016/j.jinorgbio.2019.110720"
}

Bošnjaković-Pavlović, N., Xu, X., Krstić, D. Z., Gillet, J., Wei, Y., Wu, P., Čolović, M. B.,& Spasojević-de Bire, A.. (2019). Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays. in Journal of Inorganic Biochemistry, 198, 110720.
https://doi.org/10.1016/j.jinorgbio.2019.110720

Bošnjaković-Pavlović N, Xu X, Krstić DZ, Gillet J, Wei Y, Wu P, Čolović MB, Spasojević-de Bire A. Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays. in Journal of Inorganic Biochemistry. 2019;198:110720.
doi:10.1016/j.jinorgbio.2019.110720 .

Bošnjaković-Pavlović, Nada, Xu, Xiao, Krstić, Danijela Z., Gillet, Jean-Michel, Wei, Yongge, Wu, Pingfan, Čolović, Mirjana B., Spasojević-de Bire, Anne, "Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays" in Journal of Inorganic Biochemistry, 198 (2019):110720,
https://doi.org/10.1016/j.jinorgbio.2019.110720 . .

TY  - JOUR
AU  - Yang, Peng
AU  - Ma, Tian
AU  - Lang, Zhongling
AU  - Misirlić-Denčić, Sonja
AU  - Isaković, Anđelka
AU  - Benyei, Attila
AU  - Čolović, Mirjana B.
AU  - Marković, Ivanka
AU  - Krstić, Danijela Z.
AU  - Poblet, Josep M.
AU  - Lin, Zhengguo
AU  - Kortz, Ulrich
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8499
AB  - The first two examples of polyoxopalladates(II) (POPs) containing tetravalent metal ion guests, [MO8Pd12(PO4)8]12- (M = SnIV, PbIV), have been prepared and structurally characterized in the solid state, solution, and gas phase. The interactions of the metal ion guests and the palladium-oxo shell were studied by theoretical calculations. The POPs were shown to possess anticancer activity by causing oxidative stress inducing caspase activation and consecutive apoptosis of leukemic cells.
T2  - Inorganic Chemistry
T1  - Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV )
VL  - 58
IS  - 17
SP  - 11294
EP  - 11299
DO  - 10.1021/acs.inorgchem.9b01129
ER  - 

@article{
author = "Yang, Peng and Ma, Tian and Lang, Zhongling and Misirlić-Denčić, Sonja and Isaković, Anđelka and Benyei, Attila and Čolović, Mirjana B. and Marković, Ivanka and Krstić, Danijela Z. and Poblet, Josep M. and Lin, Zhengguo and Kortz, Ulrich",
year = "2019",
abstract = "The first two examples of polyoxopalladates(II) (POPs) containing tetravalent metal ion guests, [MO8Pd12(PO4)8]12- (M = SnIV, PbIV), have been prepared and structurally characterized in the solid state, solution, and gas phase. The interactions of the metal ion guests and the palladium-oxo shell were studied by theoretical calculations. The POPs were shown to possess anticancer activity by causing oxidative stress inducing caspase activation and consecutive apoptosis of leukemic cells.",
journal = "Inorganic Chemistry",
title = "Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV )",
volume = "58",
number = "17",
pages = "11294-11299",
doi = "10.1021/acs.inorgchem.9b01129"
}

Yang, P., Ma, T., Lang, Z., Misirlić-Denčić, S., Isaković, A., Benyei, A., Čolović, M. B., Marković, I., Krstić, D. Z., Poblet, J. M., Lin, Z.,& Kortz, U.. (2019). Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV ). in Inorganic Chemistry, 58(17), 11294-11299.
https://doi.org/10.1021/acs.inorgchem.9b01129

Yang P, Ma T, Lang Z, Misirlić-Denčić S, Isaković A, Benyei A, Čolović MB, Marković I, Krstić DZ, Poblet JM, Lin Z, Kortz U. Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV ). in Inorganic Chemistry. 2019;58(17):11294-11299.
doi:10.1021/acs.inorgchem.9b01129 .

Yang, Peng, Ma, Tian, Lang, Zhongling, Misirlić-Denčić, Sonja, Isaković, Anđelka, Benyei, Attila, Čolović, Mirjana B., Marković, Ivanka, Krstić, Danijela Z., Poblet, Josep M., Lin, Zhengguo, Kortz, Ulrich, "Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV )" in Inorganic Chemistry, 58, no. 17 (2019):11294-11299,
https://doi.org/10.1021/acs.inorgchem.9b01129 . .

TY  - JOUR
AU  - Kornjača, Duško
AU  - Živković, Vladimir I.
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Marko
AU  - Stanković, Sanja
AU  - Mutavdžin, Slavica
AU  - Jakovljević, Vladimir Lj.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700041K
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7714
AB  - The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.
T2  - Archives of Biological Sciences
T1  - The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat
VL  - 70
IS  - 2
SP  - 241
EP  - 248
DO  - 10.2298/ABS170731041K
ER  - 

@article{
author = "Kornjača, Duško and Živković, Vladimir I. and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Marko and Stanković, Sanja and Mutavdžin, Slavica and Jakovljević, Vladimir Lj. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.",
journal = "Archives of Biological Sciences",
title = "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat",
volume = "70",
number = "2",
pages = "241-248",
doi = "10.2298/ABS170731041K"
}

Kornjača, D., Živković, V. I., Krstić, D. Z., Čolović, M. B., Đurić, M., Stanković, S., Mutavdžin, S., Jakovljević, V. Lj.,& Đurić, D. M.. (2018). The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences, 70(2), 241-248.
https://doi.org/10.2298/ABS170731041K

Kornjača D, Živković VI, Krstić DZ, Čolović MB, Đurić M, Stanković S, Mutavdžin S, Jakovljević VL, Đurić DM. The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences. 2018;70(2):241-248.
doi:10.2298/ABS170731041K .

Kornjača, Duško, Živković, Vladimir I., Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Marko, Stanković, Sanja, Mutavdžin, Slavica, Jakovljević, Vladimir Lj., Đurić, Dragan M., "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat" in Archives of Biological Sciences, 70, no. 2 (2018):241-248,
https://doi.org/10.2298/ABS170731041K . .

TY  - JOUR
AU  - Jakovljević-Uzelac, Jovana
AU  - Stanić, Marina
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-017-3238-z
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7788
AB  - The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.
T2  - Molecular and Cellular Biochemistry
T1  - Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters
VL  - 444
IS  - 1-2
SP  - 143
EP  - 148
DO  - 10.1007/s11010-017-3238-z
ER  - 

@article{
author = "Jakovljević-Uzelac, Jovana and Stanić, Marina and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.",
journal = "Molecular and Cellular Biochemistry",
title = "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters",
volume = "444",
number = "1-2",
pages = "143-148",
doi = "10.1007/s11010-017-3238-z"
}

Jakovljević-Uzelac, J., Stanić, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry, 444(1-2), 143-148.
https://doi.org/10.1007/s11010-017-3238-z

Jakovljević-Uzelac J, Stanić M, Krstić DZ, Čolović MB, Đurić DM. Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry. 2018;444(1-2):143-148.
doi:10.1007/s11010-017-3238-z .

Jakovljević-Uzelac, Jovana, Stanić, Marina, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters" in Molecular and Cellular Biochemistry, 444, no. 1-2 (2018):143-148,
https://doi.org/10.1007/s11010-017-3238-z . .

TY  - CONF
AU  - Đurić, Aleksandar
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Obrenović, Radmila
AU  - Micovic, Z
AU  - Đurić, Marija
AU  - Đurić, Dragan M.
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018309547
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7954
C3  - Atherosclerosis
T1  - The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood
VL  - 275
SP  - e206
EP  - e207
DO  - 10.1016/j.atherosclerosis.2018.06.642
ER  - 

@conference{
author = "Đurić, Aleksandar and Čolović, Mirjana B. and Krstić, Danijela Z. and Obrenović, Radmila and Micovic, Z and Đurić, Marija and Đurić, Dragan M.",
year = "2018",
journal = "Atherosclerosis",
title = "The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood",
volume = "275",
pages = "e206-e207",
doi = "10.1016/j.atherosclerosis.2018.06.642"
}

Đurić, A., Čolović, M. B., Krstić, D. Z., Obrenović, R., Micovic, Z., Đurić, M.,& Đurić, D. M.. (2018). The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood. in Atherosclerosis, 275, e206-e207.
https://doi.org/10.1016/j.atherosclerosis.2018.06.642

Đurić A, Čolović MB, Krstić DZ, Obrenović R, Micovic Z, Đurić M, Đurić DM. The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood. in Atherosclerosis. 2018;275:e206-e207.
doi:10.1016/j.atherosclerosis.2018.06.642 .

Đurić, Aleksandar, Čolović, Mirjana B., Krstić, Danijela Z., Obrenović, Radmila, Micovic, Z, Đurić, Marija, Đurić, Dragan M., "The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood" in Atherosclerosis, 275 (2018):e206-e207,
https://doi.org/10.1016/j.atherosclerosis.2018.06.642 . .

TY  - JOUR
AU  - Dinčić, Marko
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Nešović Ostojić, Jelena
AU  - Kovačević, Sanjin
AU  - De Luka, Silvio R.
AU  - Đorđević, Drago M.
AU  - Ćirković, Saša
AU  - Brkić, Predrag
AU  - Todorović, Jasna
PY  - 2018
UR  - https://www.tandfonline.com/doi/full/10.1080/09553002.2018.1518611
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7957
AB  - Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.
T2  - International Journal of Radiation Biology
T1  - Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field
VL  - 94
IS  - 11
SP  - 1062
EP  - 1071
DO  - 10.1080/09553002.2018.1518611
ER  - 

@article{
author = "Dinčić, Marko and Krstić, Danijela Z. and Čolović, Mirjana B. and Nešović Ostojić, Jelena and Kovačević, Sanjin and De Luka, Silvio R. and Đorđević, Drago M. and Ćirković, Saša and Brkić, Predrag and Todorović, Jasna",
year = "2018",
abstract = "Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.",
journal = "International Journal of Radiation Biology",
title = "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field",
volume = "94",
number = "11",
pages = "1062-1071",
doi = "10.1080/09553002.2018.1518611"
}

Dinčić, M., Krstić, D. Z., Čolović, M. B., Nešović Ostojić, J., Kovačević, S., De Luka, S. R., Đorđević, D. M., Ćirković, S., Brkić, P.,& Todorović, J.. (2018). Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. in International Journal of Radiation Biology, 94(11), 1062-1071.
https://doi.org/10.1080/09553002.2018.1518611

Dinčić M, Krstić DZ, Čolović MB, Nešović Ostojić J, Kovačević S, De Luka SR, Đorđević DM, Ćirković S, Brkić P, Todorović J. Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. in International Journal of Radiation Biology. 2018;94(11):1062-1071.
doi:10.1080/09553002.2018.1518611 .

Dinčić, Marko, Krstić, Danijela Z., Čolović, Mirjana B., Nešović Ostojić, Jelena, Kovačević, Sanjin, De Luka, Silvio R., Đorđević, Drago M., Ćirković, Saša, Brkić, Predrag, Todorović, Jasna, "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field" in International Journal of Radiation Biology, 94, no. 11 (2018):1062-1071,
https://doi.org/10.1080/09553002.2018.1518611 . .

TY  - JOUR
AU  - Stojanović, Marija
AU  - Todorović, Dajana D.
AU  - Šćepanović, Ljiljana
AU  - Mitrović, Dušan M.
AU  - Borozan, Sunčica Z.
AU  - Dragutinović, Vesna V.
AU  - Labudović-Borović, Milica
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-018-3311-2
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8060
AB  - The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.
T2  - Molecular and Cellular Biochemistry
T1  - Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue
VL  - 448
IS  - 1-2
SP  - 43
EP  - 50
DO  - 10.1007/s11010-018-3311-2
ER  - 

@article{
author = "Stojanović, Marija and Todorović, Dajana D. and Šćepanović, Ljiljana and Mitrović, Dušan M. and Borozan, Sunčica Z. and Dragutinović, Vesna V. and Labudović-Borović, Milica and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.",
journal = "Molecular and Cellular Biochemistry",
title = "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue",
volume = "448",
number = "1-2",
pages = "43-50",
doi = "10.1007/s11010-018-3311-2"
}

Stojanović, M., Todorović, D. D., Šćepanović, L., Mitrović, D. M., Borozan, S. Z., Dragutinović, V. V., Labudović-Borović, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry, 448(1-2), 43-50.
https://doi.org/10.1007/s11010-018-3311-2

Stojanović M, Todorović DD, Šćepanović L, Mitrović DM, Borozan SZ, Dragutinović VV, Labudović-Borović M, Krstić DZ, Čolović MB, Đurić DM. Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry. 2018;448(1-2):43-50.
doi:10.1007/s11010-018-3311-2 .

Stojanović, Marija, Todorović, Dajana D., Šćepanović, Ljiljana, Mitrović, Dušan M., Borozan, Sunčica Z., Dragutinović, Vesna V., Labudović-Borović, Milica, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue" in Molecular and Cellular Biochemistry, 448, no. 1-2 (2018):43-50,
https://doi.org/10.1007/s11010-018-3311-2 . .

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1926
AB  - Background: Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in protein structure. Sulphur amino acids are involved in the synthesis of intracellular antioxidants such as glutathione and N-acetyl cysteine. Moreover, naturally occurring sulphur-containing ligands are effective and safe detoxifying agents, often used in order to prevent toxic metal ions effects and their accumulation in human body. Methods: Literature search for peer-reviewed articles was performed using PubMed and Scopus databases, and utilizing appropriate keywords. Results: This review is focused on sulphur-containing amino acids - methionine, cysteine, taurine, and their derivatives - glutathione and N-acetylcysteine, and their defense effects as antioxidant agents against free radicals. Additionally, the protective effects of sulphur-containing ligands against the toxic effects of heavy and transition metal ions, and their reactivation role towards the enzyme inhibition are described. Conclusion: Sulphur-containing amino acids represent a powerful part of cell antioxidant system. Thus, they are essential in the maintenance of normal cellular functions and health. In addition to their worthy antioxidant action, sulphur-containing amino acids may offer a chelating site for heavy metals. Accordingly, they may be supplemented during chelating therapy, providing beneficial effects in eliminating toxic metals.
T2  - Current Medicinal Chemistry
T1  - Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals
VL  - 25
IS  - 3
SP  - 324
EP  - 335
DO  - 10.2174/0929867324666170609075434
ER  - 

@article{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2018",
abstract = "Background: Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in protein structure. Sulphur amino acids are involved in the synthesis of intracellular antioxidants such as glutathione and N-acetyl cysteine. Moreover, naturally occurring sulphur-containing ligands are effective and safe detoxifying agents, often used in order to prevent toxic metal ions effects and their accumulation in human body. Methods: Literature search for peer-reviewed articles was performed using PubMed and Scopus databases, and utilizing appropriate keywords. Results: This review is focused on sulphur-containing amino acids - methionine, cysteine, taurine, and their derivatives - glutathione and N-acetylcysteine, and their defense effects as antioxidant agents against free radicals. Additionally, the protective effects of sulphur-containing ligands against the toxic effects of heavy and transition metal ions, and their reactivation role towards the enzyme inhibition are described. Conclusion: Sulphur-containing amino acids represent a powerful part of cell antioxidant system. Thus, they are essential in the maintenance of normal cellular functions and health. In addition to their worthy antioxidant action, sulphur-containing amino acids may offer a chelating site for heavy metals. Accordingly, they may be supplemented during chelating therapy, providing beneficial effects in eliminating toxic metals.",
journal = "Current Medicinal Chemistry",
title = "Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals",
volume = "25",
number = "3",
pages = "324-335",
doi = "10.2174/0929867324666170609075434"
}

Čolović, M. B., Vasić, V. M., Đurić, D. M.,& Krstić, D. Z.. (2018). Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals. in Current Medicinal Chemistry, 25(3), 324-335.
https://doi.org/10.2174/0929867324666170609075434

Čolović MB, Vasić VM, Đurić DM, Krstić DZ. Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals. in Current Medicinal Chemistry. 2018;25(3):324-335.
doi:10.2174/0929867324666170609075434 .

Čolović, Mirjana B., Vasić, Vesna M., Đurić, Dragan M., Krstić, Danijela Z., "Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals" in Current Medicinal Chemistry, 25, no. 3 (2018):324-335,
https://doi.org/10.2174/0929867324666170609075434 . .

TY  - CONF
AU  - Jakovljević-Uzelac, Jovana
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Đurić, Marko
AU  - Đurić, Dragan M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7175
C3  - Atherosclerosis
T1  - The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma
VL  - 263
SP  - E137
EP  - E138
DO  - 10.1016/j.atherosclerosis.2017.06.440
ER  - 

@conference{
author = "Jakovljević-Uzelac, Jovana and Čolović, Mirjana B. and Krstić, Danijela Z. and Đurić, Marko and Đurić, Dragan M.",
year = "2017",
journal = "Atherosclerosis",
title = "The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma",
volume = "263",
pages = "E137-E138",
doi = "10.1016/j.atherosclerosis.2017.06.440"
}

Jakovljević-Uzelac, J., Čolović, M. B., Krstić, D. Z., Đurić, M.,& Đurić, D. M.. (2017). The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma. in Atherosclerosis, 263, E137-E138.
https://doi.org/10.1016/j.atherosclerosis.2017.06.440

Jakovljević-Uzelac J, Čolović MB, Krstić DZ, Đurić M, Đurić DM. The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma. in Atherosclerosis. 2017;263:E137-E138.
doi:10.1016/j.atherosclerosis.2017.06.440 .

Jakovljević-Uzelac, Jovana, Čolović, Mirjana B., Krstić, Danijela Z., Đurić, Marko, Đurić, Dragan M., "The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma" in Atherosclerosis, 263 (2017):E137-E138,
https://doi.org/10.1016/j.atherosclerosis.2017.06.440 . .