TY  - JOUR
AU  - Jovanović, Željka
AU  - Radosavljević, Aleksandra
AU  - Stojkovska, Jasmina
AU  - Nikolić, Branislav Ž.
AU  - Obradović, Bojana
AU  - Kačarević-Popović, Zorica M.
AU  - Mišković-Stanković, Vesna B.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5834
AB  - Silver nanoparticles were produced inside a poly(N-vinyl-2-pyrrolidone) hydrogel (PVP) by an innovative method based on the electrochemical reduction of Ag+ ions within the swollen PVP hydrogel. UV-visible spectroscopy showed the highest value of the absorbance intensity and the lowest values of the wavelength of the absorbance maximum and the full width at the half-maximum absorbance for the Ag/PVP nanocomposite obtained at 200 V during 4 min. Cyclic voltammetry results suggested an adequate entrapment of the silver nanoparticles. The mechanical properties under bioreactor conditions of the Ag/PVP nanocomposite suggested the possibility of wound dressing application. Silver release from Ag/PVP nanocomposites was confirmed under static conditions as well as by their antimicrobial activity against Staphylococcus aureus. POLYM. COMPOS., 35:217-226, 2014. (c) 2013 Society of Plastics Engineers
T2  - Polymer Composites
T1  - Silver/Poly(N-vinyl-2-pyrrolidone) Hydrogel Nanocomposites Obtained by Electrochemical Synthesis of Silver Nanoparticles Inside the Polymer Hydrogel Aimed for Biomedical Applications
VL  - 35
IS  - 2
SP  - 217
EP  - 226
DO  - 10.1002/pc.22653
ER  - 

@article{
author = "Jovanović, Željka and Radosavljević, Aleksandra and Stojkovska, Jasmina and Nikolić, Branislav Ž. and Obradović, Bojana and Kačarević-Popović, Zorica M. and Mišković-Stanković, Vesna B.",
year = "2014",
abstract = "Silver nanoparticles were produced inside a poly(N-vinyl-2-pyrrolidone) hydrogel (PVP) by an innovative method based on the electrochemical reduction of Ag+ ions within the swollen PVP hydrogel. UV-visible spectroscopy showed the highest value of the absorbance intensity and the lowest values of the wavelength of the absorbance maximum and the full width at the half-maximum absorbance for the Ag/PVP nanocomposite obtained at 200 V during 4 min. Cyclic voltammetry results suggested an adequate entrapment of the silver nanoparticles. The mechanical properties under bioreactor conditions of the Ag/PVP nanocomposite suggested the possibility of wound dressing application. Silver release from Ag/PVP nanocomposites was confirmed under static conditions as well as by their antimicrobial activity against Staphylococcus aureus. POLYM. COMPOS., 35:217-226, 2014. (c) 2013 Society of Plastics Engineers",
journal = "Polymer Composites",
title = "Silver/Poly(N-vinyl-2-pyrrolidone) Hydrogel Nanocomposites Obtained by Electrochemical Synthesis of Silver Nanoparticles Inside the Polymer Hydrogel Aimed for Biomedical Applications",
volume = "35",
number = "2",
pages = "217-226",
doi = "10.1002/pc.22653"
}

Jovanović, Ž., Radosavljević, A., Stojkovska, J., Nikolić, B. Ž., Obradović, B., Kačarević-Popović, Z. M.,& Mišković-Stanković, V. B.. (2014). Silver/Poly(N-vinyl-2-pyrrolidone) Hydrogel Nanocomposites Obtained by Electrochemical Synthesis of Silver Nanoparticles Inside the Polymer Hydrogel Aimed for Biomedical Applications. in Polymer Composites, 35(2), 217-226.
https://doi.org/10.1002/pc.22653

Jovanović Ž, Radosavljević A, Stojkovska J, Nikolić BŽ, Obradović B, Kačarević-Popović ZM, Mišković-Stanković VB. Silver/Poly(N-vinyl-2-pyrrolidone) Hydrogel Nanocomposites Obtained by Electrochemical Synthesis of Silver Nanoparticles Inside the Polymer Hydrogel Aimed for Biomedical Applications. in Polymer Composites. 2014;35(2):217-226.
doi:10.1002/pc.22653 .

Jovanović, Željka, Radosavljević, Aleksandra, Stojkovska, Jasmina, Nikolić, Branislav Ž., Obradović, Bojana, Kačarević-Popović, Zorica M., Mišković-Stanković, Vesna B., "Silver/Poly(N-vinyl-2-pyrrolidone) Hydrogel Nanocomposites Obtained by Electrochemical Synthesis of Silver Nanoparticles Inside the Polymer Hydrogel Aimed for Biomedical Applications" in Polymer Composites, 35, no. 2 (2014):217-226,
https://doi.org/10.1002/pc.22653 . .

TY  - JOUR
AU  - Jovanović, Željka
AU  - Radosavljević, Aleksandra
AU  - Kačarević-Popović, Zorica M.
AU  - Stojkovska, Jasmina
AU  - Perić-Grujić, Aleksandra A.
AU  - Ristić, Mirjana
AU  - Matić, Ivana Z.
AU  - Juranić, Zorica D.
AU  - Obradović, Bojana
AU  - Mišković-Stanković, Vesna B.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5379
AB  - Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites
VL  - 105
SP  - 230
EP  - 235
DO  - 10.1016/j.colsurfb.2012.12.055
ER  - 

@article{
author = "Jovanović, Željka and Radosavljević, Aleksandra and Kačarević-Popović, Zorica M. and Stojkovska, Jasmina and Perić-Grujić, Aleksandra A. and Ristić, Mirjana and Matić, Ivana Z. and Juranić, Zorica D. and Obradović, Bojana and Mišković-Stanković, Vesna B.",
year = "2013",
abstract = "Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites",
volume = "105",
pages = "230-235",
doi = "10.1016/j.colsurfb.2012.12.055"
}

Jovanović, Ž., Radosavljević, A., Kačarević-Popović, Z. M., Stojkovska, J., Perić-Grujić, A. A., Ristić, M., Matić, I. Z., Juranić, Z. D., Obradović, B.,& Mišković-Stanković, V. B.. (2013). Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites. in Colloids and Surfaces. B: Biointerfaces, 105, 230-235.
https://doi.org/10.1016/j.colsurfb.2012.12.055

Jovanović Ž, Radosavljević A, Kačarević-Popović ZM, Stojkovska J, Perić-Grujić AA, Ristić M, Matić IZ, Juranić ZD, Obradović B, Mišković-Stanković VB. Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites. in Colloids and Surfaces. B: Biointerfaces. 2013;105:230-235.
doi:10.1016/j.colsurfb.2012.12.055 .

Jovanović, Željka, Radosavljević, Aleksandra, Kačarević-Popović, Zorica M., Stojkovska, Jasmina, Perić-Grujić, Aleksandra A., Ristić, Mirjana, Matić, Ivana Z., Juranić, Zorica D., Obradović, Bojana, Mišković-Stanković, Vesna B., "Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites" in Colloids and Surfaces. B: Biointerfaces, 105 (2013):230-235,
https://doi.org/10.1016/j.colsurfb.2012.12.055 . .

TY  - JOUR
AU  - Jovanović, Željka
AU  - Krklješ, Aleksandra N.
AU  - Stojkovska, Jasmina
AU  - Tomić, Simonida Lj.
AU  - Obradović, Bojana
AU  - Mišković-Stanković, Vesna B.
AU  - Kačarević-Popović, Zorica M.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4471
AB  - This work describes radiolytic synthesis of silver nanoparticles (Ag NPs) within the poly(N-vinyl-2-pyrrolidone) (PVP) hydrogel. The hydrogel matrix was obtained by gamma irradiation-induced cross-linking, while the in situ reduction of Ag(+) ions was performed using strong reducing species formed under water radiolysis. Absorption spectrum of the Ag/PVP nanocomposite confirmed the formation of Ag NPs, showing the surface plasmon band maxima at 405 nm. Ag/PVP nanocomposites were characterized by XRD and TEM analysis, accompanied with investigations of swelling and diffusion properties in the simulated body fluid at 37 degrees C, and mechanical properties in bioreactor conditions. It was shown that Ag/PVP nanocomposite exhibited higher values of equilibrium swelling degree, Youngs modulus, and molar mass between crosslinks, while lower values of the diffusion coefficient and effective crosslink density were obtained, as compared to the pure PVP. (C) 2011 Elsevier Ltd. All rights reserved.
T2  - Radiation Physics and Chemistry
T1  - Synthesis and characterization of silver/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposite obtained by in situ radiolytic method
VL  - 80
IS  - 11
SP  - 1208
EP  - 1215
DO  - 10.1016/j.radphyschem.2011.06.005
ER  - 

@article{
author = "Jovanović, Željka and Krklješ, Aleksandra N. and Stojkovska, Jasmina and Tomić, Simonida Lj. and Obradović, Bojana and Mišković-Stanković, Vesna B. and Kačarević-Popović, Zorica M.",
year = "2011",
abstract = "This work describes radiolytic synthesis of silver nanoparticles (Ag NPs) within the poly(N-vinyl-2-pyrrolidone) (PVP) hydrogel. The hydrogel matrix was obtained by gamma irradiation-induced cross-linking, while the in situ reduction of Ag(+) ions was performed using strong reducing species formed under water radiolysis. Absorption spectrum of the Ag/PVP nanocomposite confirmed the formation of Ag NPs, showing the surface plasmon band maxima at 405 nm. Ag/PVP nanocomposites were characterized by XRD and TEM analysis, accompanied with investigations of swelling and diffusion properties in the simulated body fluid at 37 degrees C, and mechanical properties in bioreactor conditions. It was shown that Ag/PVP nanocomposite exhibited higher values of equilibrium swelling degree, Youngs modulus, and molar mass between crosslinks, while lower values of the diffusion coefficient and effective crosslink density were obtained, as compared to the pure PVP. (C) 2011 Elsevier Ltd. All rights reserved.",
journal = "Radiation Physics and Chemistry",
title = "Synthesis and characterization of silver/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposite obtained by in situ radiolytic method",
volume = "80",
number = "11",
pages = "1208-1215",
doi = "10.1016/j.radphyschem.2011.06.005"
}

Jovanović, Ž., Krklješ, A. N., Stojkovska, J., Tomić, S. Lj., Obradović, B., Mišković-Stanković, V. B.,& Kačarević-Popović, Z. M.. (2011). Synthesis and characterization of silver/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposite obtained by in situ radiolytic method. in Radiation Physics and Chemistry, 80(11), 1208-1215.
https://doi.org/10.1016/j.radphyschem.2011.06.005

Jovanović Ž, Krklješ AN, Stojkovska J, Tomić SL, Obradović B, Mišković-Stanković VB, Kačarević-Popović ZM. Synthesis and characterization of silver/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposite obtained by in situ radiolytic method. in Radiation Physics and Chemistry. 2011;80(11):1208-1215.
doi:10.1016/j.radphyschem.2011.06.005 .

Jovanović, Željka, Krklješ, Aleksandra N., Stojkovska, Jasmina, Tomić, Simonida Lj., Obradović, Bojana, Mišković-Stanković, Vesna B., Kačarević-Popović, Zorica M., "Synthesis and characterization of silver/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposite obtained by in situ radiolytic method" in Radiation Physics and Chemistry, 80, no. 11 (2011):1208-1215,
https://doi.org/10.1016/j.radphyschem.2011.06.005 . .